===========================
Semi-direct Phosphorylation
===========================

on S183 S212? S221

  *** rPras40[32P] phos[32P-ATP] is increased by Raptor[Ab]IP
    *** cells: none
    *** IPfrom: 3T3L1 in BMLS "differentiated into adipocytes"
    *** partially inhibited by: rPras40(S183A) [substitution]
    *** unaffected by: rPras40(T246A) [substitution]
    *** source: 18372248-Fig-1a
  *** rPras40[32P] phos[32P-ATP] is increased by Raptor[Ab]IP
    *** cells: none
    *** IPfrom: 3T3L1 in BMLS "differentiated into adipocytes"
    *** partially inhibited by: rPras40(S212A) [substitution]
    *** partially inhibited by: rPras40(S221A) [substitution]
    *** inhibited by: rPras40(S183A/S212A/S221A) [substitution]
    *** source: 18372248-Fig-1d
  *** rPras40[32P] phos[32P-ATP] is increased by Raptor[Ab]IP
    *** cells: none
    *** IPfrom: HEK293E in BMS
    *** unaffected by: rPras40(S212A) [substitution]
    *** partially inhibited by: rPras40(S183A/S212A/S221A) [substitution]
    *** source: 18372248-Fig-1e


  *** rPras40[32P] phos[32P-ATP] is increased by Raptor[Ab]IP
    *** cells: none
    *** IPfrom: 3T3L1 in BMLS "differentiated into adipocytes"
    *** comment: inhibited by disruption of Mtor/Raptor complex with Triton X100
    *** source: 18372248-Fig-3a
  *** rPras40[32P] phos[32P-ATP] is increased by Raptor[Ab]IP
    *** cells: none
    *** IPfrom: 3T3L1 in BMLS "differentiated into adipocytes"
    *** unaffected by: rPras40(F129A) [substitution]
    *** enhanced by: rPras40(P185A) [substitution]
    *** inhibited by: rPras40(F129A/P185A) [substitution]
    *** source: 18372248-Fig-3b
  *** rPras40[32P] phos[32P-ATP] is increased by Raptor[Ab]IP
    *** cells: none
    *** IPfrom: HEK293E in BMS
    *** comment: inhibited by treatment of IP with LY294002
    *** comment: unaffected by treatment of IP with Rapamycin
    *** comment: unaffected by treatment of IP with rFkbp12
    *** comment: inhibited by treatment of IP with (Rapamycin + rFkbp12)
    *** source: 18372248-Fig-1e

