=================
Copreciptitation
=================

  *** xTp53[Ab] copptby[WB] xPin1[Ab] is detectable
    *** cells: SAOS2<xTp53><xPin1> in BMS
    *** source: 12397362-Fig-S1b

requires some sort of phos

  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with UV"
    *** comment: snagged Tp53 is phosed on S15 [phosAb]
    *** comment: inhibited by treatment of lysates with phosphatase"
    *** source: 12397362-Fig-1c
  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with ActinomycinD"
    *** comment: enhanced by treatment of lysates with Ptpase inhibitors
    *** source: 12397361-Fig-1d
  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with Adriamycin"
    *** comment: enhanced by treatment of lysates with Ptpase inhibitors
    *** source: 12397361-Fig-1d
  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with Camptothecin"
    *** comment: enhanced by treatment of lysates with Ptpase inhibitors
    *** source: 12397361-Fig-1d

requires Tp53 S315 and T81 and S33 phos

  *** xTp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS<xTp53> in BMS "treated with UV"
    *** unaffected by: xTp53(S46A) [substitution]
    *** partially inhibited by: xTp53(T81A) [substitution]
    *** partially inhibited by: xTp53(S33A) [substitution]
    *** partially inhibited by: xTp53(S315A) [substitution]
    *** inhibited by: xTp53(T81A/S315A) [substitution]
    *** inhibited by: xTp53(S33A/S315A) [substitution]
    *** inhibited by: xTp53(S33A/T81A/S315A) [substitution]
    *** source: 12397362-Fig-2a
  *** xTp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS<xTp53> in BMS "treated with UV"
    *** partially inhibited by: xTp53(S33A/S315A) [substitution]
    *** inhibited by: xTp53(S33A/S315A/T81A) [substitution]
    *** source: 12397361-Fig-1e
  *** xTp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: SAOS2<Tp53~null><xTp53> in BMS "treated with Adriamycin"
    *** partially inhibited by: xTp53(S33A) [substitution]
    *** unaffected by: xTp53(S46A) [substitution]
    *** unaffected by: xTp53(T81A) [substitution]
    *** partially inhibited by: xTp53(S315A) [substitution]
    *** inhibited by: xTp53(S33A/S315A) [substitution]
    *** source: 12397361-Fig-S1b
  *** xTp53[Ab] copptby[WB] Pin1[Ab] is detectable
    *** cells: U2OS<xTp53> in BMS "treated with UV"
    *** inhibited by: xTp53(S33A/S315A/T81A) [substitution]
    *** source: 12397361-Fig-1e
  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with UV"
    *** comment: snagged Tp53 is phosed on T81
    *** source: 12397362-Fig-S1c
  *** xTp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: SAOS2<Tp53~null><xTp53> in BMS "treated with Etoposide for 12 hr"
    *** partially inhibited by: xTp53(S33A/S46A/T81A/S315A) [substitution]
    *** inhibited by: xTp53(S33A/S46A/T81A/S127A/T150A/S315A) [substitution]
    *** source: 17906639-Fig-3a
  *** xTp53[Ab]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** partially inhibited by: xTp53(S33A/S46A/T81A/S315A) [substitution]
    *** inhibited by: xTp53(S33A/S46A/T81A/S127A/T150A/S315A) [substitution]
    *** source: 17906639-Fig-4b
  *** xTp53(S33A/S46A/T81A/S127A/T150A)"S315"[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(S46A/T81A/S127A/T150A/S315A)"S33" [substitution]
    *** unaffected by: xTp53(S33A/S46A/S127A/T150A/S315A)"T81" [substitution]
    *** inhibited by: xTp53(S33A/S46A/T81A/S127A/T150A/S315A) [substitution]
    *** partially inhibited by: xTp53(S33A/T81A/S127A/T150A/S315A)"S46" [substitution]
    *** source: 17906639-Fig-S2b

does not require Tp53 acetyl sites

  *** xTp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(K319R/K320R/K321R/K370R/K372R/K373R/K381R/K382R/K386R)"9KR" [substitution] 
    *** source: 17906639-Fig-S5a

enhanced by Tp53(P27R)

  *** xTp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: H1299<Tp53~null><xTp53> in BMS "treated with UV for 12 hr"
    *** enhanced by: xTp53(P72R) [substitution]
    *** source: 17906639-Fig-S6c

requires Pin1 WWW-domain(1-38), Y23, W34  but not Isomerase activity

  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with UV"
    *** unaffected by: rPin1(1-38)"WW-domain" [substitution]
    *** inhibited by: rPin1(38-163)"PpiC-domain" [substitution]
    *** inhibited by: rPin1(Y23A) [substitution]
    *** unaffected by: rPin1(C109A)"CIA" [substitution]
    *** source: 12397362-Fig-S1a
  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with Adriamycin"
    *** partially inhibited by: rPin1(mnr)"WWW-domain" [substitution]
    *** inhibited by: rPin1(mnr)"PPIC-domain" [substitution]
    *** inhibited by: rPin1(W34A) [substitution]
    *** source: 12397361-Fig-1b


no extras--

  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with Adriamycin"
    *** source: 12397361-Fig-1c
  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with ALLN"
    *** source: 12397361-Fig-1c
  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with Camptothecin"
    *** source: 12397361-Fig-1c
  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is detectable
    *** cells: U2OS in BMS "treated with UV"
    *** source: 12397361-Fig-1c
  *** Tp53[Ab] copptby[WB] Pin1[Ab] is detectable
    *** cells: U2OS in BMS "treated with Adriamycin"
    *** source: 12397361-Fig-1b











