=================
Direct Activation
=================

  *** rPak1 IVKA(HistH4)[32P-ATP] is increased by rCdc42{GTPgS}
    *** cells: none
    *** source: 16129686-Fig-1a
  *** rPak1 IVKA(auto)[32P-ATP] is increased by rCdc42{GTPgS}
    *** cells: none
    *** source: 16129686-Fig-1a
  *** xPak1[tAb]IP IVKA(MBP)[32P-ATP] is increased by rCdc42{GTPgS}
    *** cells: none
    *** IPfrom: COS7 in BMS
    *** source: 9032240-Fig-2c

===========================
Semi-direct Phosphorylation
===========================

  *** xPak1[tAb]IP phos(T423)[phosAb] is increased by rCdc42{GTPgS}
    *** cells: none
    *** comment: source of xPak1-IP was not reported
    *** inhibited by: xPak1(T423A) [substitution]
    *** inhibited by: xPak1(T423E) [substitution]
    *** source: 10995762-Fig-2a
  *** xPak1[tAb]IP phos(T423)[phosAb] is increased by rCdc42{GTPgS}
    *** cells: none
    *** comment: source of xPak1-IP was not reported
    *** comment: xPak-IP can also phos rPak1(K299A) on T423
    *** source: 10995762-Fig-2b

==============================================
activation by cotransfection of Cdc42
==============================================

  *** xPak1[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xCdc42(G12V)"CA"
    *** cells: COS7 in BMS
    *** source: 9032240-Fig-2a
  *** xPak1[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xCdc42(G12V)"CA"
    *** cells: COS7 in BMS
    *** source: 8805275-Fig-3
  *** xPak1[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xCdc42(Q61L)"CA"
    *** cells: COS7 in BMS
    *** source: 8910292(D)
  *** xPak1[tAb]IP IVKA(HistH4)[32P-ATP] is increased itpo xCdc42
    *** cells: COS7 in BMS
    *** enhanced by: xArhGef6 [addition]
    *** source: 12887923-Fig-1c
  *** xPak1[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xCdc42(G12V)
    *** cells: COS7<xPak1> in BMS
    *** partially inhibited by: xCdc42(G12V/Y40C) [substitution]
    *** partially inhibited by: xCdc42(G12V/T35A) [substitution]
    *** source: 9659915-Fig-7c
  *** xPak1[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xCdc42(G12V/Y40C)
    *** cells: COS7<xPak1> in BMS
    *** enhanced by: xArhGef7 [addition]
    *** unaffected by: rArhGef7(W43P/W44G) [addition]
    *** source: 9659915-Fig-7c

======================================
physical interaction with active Cdc42
======================================

  *** xPak1[Ab] copptby[WB] xCdc42(Q61L)"CA"[tAb] is detectable
    *** cells: COS7 in BMS
    *** source: 8910292(D)
  *** xPak1[tAb] snaggedby[WB] rCdc42(Q61L)"CA"[GST] is detectable
    *** cells: NIH3T3 in BMS
    *** source: 12376551-Fig-2
  *** xPak1[tAb] copptby[WB] xCdc42(Q61L)"CA"[tAb] is detectable
    *** cells: COS7<xSrc> in BMS
    *** unaffected by: xSrc(Y530F) [substitution]
    *** source: 14506284-Fig-3l
  *** xPak1[tAb]IP IVKA(HistH4)[32P-ATP] is increased itpo (xArhGef6 + xCdc42)
    *** cells: COS7 in BMS
    *** enhanced by: xGnb1 + xGng2 [addition]
    *** source: 12887923-Fig-1a
  *** xCdc42(G12V)[tAb] snaggedby[WB] rPak1(mnr)"CRIB"[GST] is detectable
    *** cells: HEK293T<xCdc42(G12V)> in BMS
    *** source: 12879077-Fig-1b

====================
Pak1 phosphorylation
====================

  *** xPak1[phosAb] phos(T423)[phosAb] is increased itpo xCdc42(Q61L)"CA"
    *** cells: 3Y1 in BMS
    *** source: 16129686-Fig-1d
  *** xPak1[tAb] phos[WBMS] is increased itpo xCdc42(G12V)"CA"
    *** cells: COS7 in BMS
    *** unaffected by: xPak1(61-554) [substitution]
    *** source: 9032240-Fig-2c
  *** xPak1[tAb]IP phos[WBMS] is increased by rCdc42{GTPgS}
    *** cells: none
    *** IPfrom: COS7<xPak1> in BMS
    *** unaffected by: xPak1(P191G/R192A)"Pix-binding-defective" [substitution]
    *** source: 9659915-Fig-5b

