======================
Direct Phosphorylation
======================

on S927

  *** rNfkb1(758-967)[phosAb] phos(S927)[phosAb] is increased by rIkk2
    *** cells: none
    *** inhibited by: rNfkb1((758-967)/S927A) [substitution]
    *** unaffected by: rNfkb1((758-967)/S932A) [substitution]
    *** source: 12482991-Fig-4c
  *** rNfkb1(758-967)[phosAb] phos(S927)[phosAb] is increased by rIkk2
    *** cells: none
    *** inhibited by: rNfkb1(758-967/S927A) [substitution]
    *** source: 11297557-Fig-6c

on S932

  *** rNfkb1(758-967)[phosAb] phos(S932)[phosAb] is increased by rIkk2
    *** cells: none
    *** inhibited by: rNfkb1((758-967)/S932A) [substitution]
    *** unaffected by: rNfkb1((758-967)/S927A) [substitution]
    *** source: 12482991-Fig-4c

Total

  *** rNfkb1(436-968)"delN"[32P] phos[32P-ATP] is increased by rIkk2
    *** cells: none
    *** partially inhibited by: rNfkb1(435-968/S921A/S923A/S932A) [substitution]
    *** source: 11158290-Fig-1b
  *** rNfkb1(917-968) phos[32P-ATP] is increased by rIkk2
    *** cells: none
    *** unaffected by: rNfkb1(917-968/S921A)"M1" [substitution]
    *** partially inhibited by: rNfkb1(917-968/S923A)"M2" [substitution]
    *** inhibited by: rNfkb1(917-968/S927A)"M3" [substitution]
    *** unaffected by: rNfkb1(917-968/S932A)"M7" [substitution]
    *** partially inhibited by: rNfkb1(917-968/S921A/S923A)"M4" [substitution]
    *** inhibited by: rNfkb1(917-968/S927A/S932A)"M9" [substitution]
    *** inhibited by: rNfkb1(917-968/S923A/S927A)"M8" [substitution]
    *** inhibited by: rNfkb1(917-968/S921A/S923A/S927T/S932A)"MAAA" [substitution]
    *** inhibited by: rNfkb1(917-968/S921A/S923A/S927A)"M5" [substitution]
    *** inhibited by: rNfkb1(917-968/S927T)"M6" [substitution]
    *** source: 11158290-Fig-2

==========================
SemiDirect Phosphorylation
==========================

  *** rNfkb1[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HELA<xIkk2> in BMS
    *** comment: enhanced by treatment of cells with Tnf for 5 min
    *** source: 10469655-Fig-4b

===========================
Phosphorylation by Nemo[IP]
===========================

on S927

  *** rNfkb1(758-967)[phosAb] phos(S927)[phosAb] is increased by Nemo[Ab]IP
    *** cells: none
    *** IPfrom: HELA in BMS "treated with Tnf for 15 min"
    *** inhibited by: rNfkb1((758-967)/S927A) [substitution]
    *** unaffected by: rNfkb1((758-967)/S932A) [substitution]
    *** source: 12482991-Fig-4d
  *** rNfkb1(758-967)[phosAb] phos(S927)[phosAb] is increased by Nemo[Ab]IP
    *** cells: none
    *** IPfrom: HELA in BMS
    *** inhibited by: rNfkb1((758-967)/S927A) [substitution]
    *** unaffected by: rNfkb1((758-967)/S932A) [substitution]
    *** source: 12482991-Fig-4d

on S932

  *** rNfkb1(758-967)[phosAb] phos(S932)[phosAb] is increased by Nemo[Ab]IP
    *** cells: none
    *** IPfrom: HELA in BMS "treated with Tnf for 15 min"
    *** inhibited by: rNfkb1((758-967)/S932A) [substitution]
    *** unaffected by: rNfkb1((758-967)/S927A) [substitution]
    *** source: 12482991-Fig-4d
  *** rNfkb1(758-967)[phosAb] phos(S932)[phosAb] is unchanged by Nemo[Ab]IP
    *** cells: none
    *** IPfrom: HELA in BMS
    *** inhibited by: rNfkb1((758-967)/S932A) [substitution]
    *** unaffected by: rNfkb1((758-967)/S927A) [substitution]
    *** source: 12482991-Fig-4d
  *** rNfkb1(758-967)[phosAb] phos(S927)[phosAb] is increased by Nemo[Ab]IP
    *** cells: none
    *** IPfrom: HELA in BMS "treated with Tnf for 15 min"
    *** inhibited by: rNfkb1(758-967/S927A) [substitution]
    *** source: 11297557-Fig-6b

==========================================
Phosphorylation by overexpression of Ikk2
==========================================

  *** xNfkb1[Ab]IP phos(S927)[phosAb] is increased itpo xIkk2
    *** cells: NIH3T3<xNfkb1> in BMS "pretreated with MG132"
    *** inhibited by: xNfkb1(S927A) [substitution]
    *** enhanced by: xNfkb1(S932A) [substitution]
    *** source: 12482991-Fig-3a
  *** xNfkb1[Ab]IP phos(S932)[phosAb] is increased itpo xIkk2
    *** cells: NIH3T3<xNfkb1> in BMS "pretreated with MG132"
    *** unaffected by: xNfkb1(S927A) [substitution]
    *** inhibited by: xNfkb1(S932A) [substitution]
    *** source: 12482991-Fig-3a

=====================================================
Ikk2 is required for Nfkb1 background phosphorylation
=====================================================

  *** Nfkb1[Ab]IP phos(S927)[phosAb] is detectable
    *** cells: mEFs<xIkk2> in BMS "pretreated with MG132"
    *** reqs: xIkk2 [omission]
    *** source: 12482991-Fig-4b
  *** Nfkb1[Ab]IP phos(S927)[phosAb] is detectable
    *** cells: mEFs<Ikk1~null><Ikk2~null><xIkk2> in BMS "pretreated with MG132"
    *** reqs: xIkk2 [omission]
    *** source: 12482991-Fig-4b
  *** Nfkb1[Ab]IP phos(S932)[phosAb] is detectable
    *** cells: mEFs<xIkk2> in BMS "pretreated with MG132"
    *** reqs: xIkk2 [omission]
    *** source: 12482991-Fig-4b
  *** Nfkb1[Ab]IP phos(S932)[phosAb] is detectable
    *** cells: mEFs<Ikk1~null><Ikk2~null><xIkk2> in BMS "pretreated with MG132"
    *** reqs: xIkk2 [omission]
    *** source: 12482991-Fig-4b
  *** xNfkb1[Ab]IP phos(S927)[phosAb] is increased itpo xIkk2
    *** cells: NIH3T3<xNfkb1> in BMS "pretreated with MG132"
    *** unaffected by: xNfkb1(S921A) [substitution]
    *** unaffected by: xNfkb1(S923A) [substitution]
    *** inhibited by: xNfkb1(S927A) [substitution]
    *** unaffected by: xNfkb1(S931A) [substitution]
    *** unaffected by: xNfkb1(S932A) [substitution]
    *** source: 11297557-Fig-5a

===================
Protein Association
===================

  *** xIkk2[Ab] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xNfkb2><xIkk2> in BMS
    *** unaffected by: xNfkb1(18-968/S921A/S923A/S927A)"AAA" [substitution]
    *** unaffected by: xNfkb1(1-936)"delC1" [substitution]
    *** unaffected by: xNfkb1(1-920)"delC2" [substitution]
    *** unaffected by: xNfkb1(1-902)"delC3" [substitution]
    *** unaffected by: xNfkb1(1-850)"delC5" [substitution]
    *** inhibited by: xNfkb1(18-809)"delC5" [substitution]
    *** inhibited by: xNfkb1(18-776)"delC7" [substitution]
    *** inhibited by: xNfkb1(18-751)"delC8" [substitution]
    *** source: 11158290-Fig-6a
  *** xIkk2[tAb] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xIkk2><xNfkb1> in BMS
    *** source: 10469655-Fig-6
