============
Mlst8-Raptor
============

Mlst8-Raptor does not req Rictor

  *** Mlst8[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** does not req: Rictor [RNAi]
    *** source: 17510057-Fig-2a

===========
Mtor-Raptor
===========

Mtor-Raptor does not require Deptor

  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xDeptor [addition]
    *** unaffected by: xDeptor(dig)"13ST/A" [addition]
    *** source: 19446321-Fig-5a

Mtor-Raptor requires Mlst8  ???

  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** reqs: Mlst8 [RNAi]
    *** source: 12718876-Fig-4b
  *** xRaptor[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293T in BMS
    *** enhanced by: xMlst8 [addition]
    *** inhibited by: xMlst8(F320S) [substitution]
    *** source: 12718876-Fig-4c
  ------- contradiction -------------------------
  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** does not req: Mlst8 [RNAi]
    *** source: 17510057-Fig-2a

Mtor-Raptor requires Pld1-- or Pld2-- or PA

  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: 786O in BMS
    *** inhibited by: xPld1(K898R)"CIA" [addition]
    *** inhibited by: xPld2(K758R)"CIA" [addition]
    *** source: 19114562-Fig-3c

Mtor-Raptor is inhibited by Rapamycin

  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: Rapamycin [addition]
    *** source: 16919458-Fig-4b
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** inhibited by: Rapamycin [addition]
    *** source: 16919458-Fig-4b
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** comment: inhibited by addition of Rapamycin to lysates for 1 or 24 hr
    *** source: 16603397-Fig-S1

Mtor-Raptor is inhibited by Rictor

  *** Raptor[Ab] copptby[WB] Mtor[Ab] is increased itao Rictor [RNAi]
    *** cells: HELA in BMS
    *** source: 15268862-Fig-3c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is increased itao Rictor [RNAi]
    *** cells: HEK293T in BMS
    *** source: 15268862-Fig-3c
  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** does not req: Rictor [RNAi]
    *** source: 17510057-Fig-2a
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** does not req: Rictor [RNAi]
    *** source: 17277771-Fig-S1d

Mtor-Raptor does not require Sgk1

  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: WM35<xSgk1> in BMS
    *** unaffected by: xSgk1(S422A) [substitution]
    *** unaffected by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5b

Mtor-Raptor does not require Sin1

  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** does not req: Sin1 [RNAi]
    *** source: 17043309-Fig-3e
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: mEFs in BMLS
    *** partially reqs: Sin1 [KO]
    *** source: 16962653-Fig-5d

================
Lysis Conditions
================

Chaps OK

  *** Mtor[Ab] copptby[WB] xRaptor[tAb] is detectable
    *** cells: HEK293T in BMS
    *** comment: Coppt works in Chaps-LB but not TritonX-LB
    *** source: 15268862-Fig-1d
  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: Ppt works in Chaps-LB but not TritonX-LB
    *** source: 15268862-Fig-2b
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: Coppt works in Chaps-LB but not TritonX-LB
    *** source: 15268862-Fig-2b
  *** xRaptor[tAb] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: Coppt works in Chaps-LB but not TritonX-LB
    *** source: 15268862-Fig-1d
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** comment: Coppt works in Chaps-LB but not NP40-LB or Triton-LB
    *** source: 17043309-Fig-3a
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: Coppt reqs Chaps-LB or Xlink in Triton-LB
    *** source: 12150925-Fig-3a,3b,3c
  *** xRaptor[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293<xMtor><xRaptor> in BMLS
    *** comment: cells were lysed without detergent
    *** comment: inhibited by washing IP with 0.25-1% Chaps
    *** source: 12150926-Fig-3b
  *** Mlst8[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: Coppt works in Chaps-LB but not TritonX-LB
    *** source: 15268862-Fig-2b
  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: unaffected by washing IP in 0.2% Chaps
    *** source: 17510057-Fig-S1a
  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: inhibited by washing IP in 0.2% NP40
    *** source: 17510057-Fig-S1a

Triton not OK

  *** xRaptor[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293T in BMS
    *** comment: lysis buffer reqs 0.1% Tween-20 instead of Triton-X-100
    *** source: 12665511-Fig-9
  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: inhibited by washing IP in 0.2% TritonX100
    *** source: 17510057-Fig-S1a
  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: inhibited by use of TritonX instead of Chaps in lysis buffer
    *** source: 17277771-Fig-S3d
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: inhibited by use of TritonX instead of Chaps in lysis buffer
    *** source: 17277771-Fig-S3c

NP40 not OK

  *** xRaptor[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293<xMtor><xRaptor> in BMLS
    *** comment: inhibited by addition of 1% NP50 to the lysis buffer
    *** comment: unaffected by Serum-deprivation and/or AA-deprivation
    *** unaffected by: Rapamycin [addition]
    *** source: 12150926-Fig-2a
  *** xRaptor[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293<xMtor><xRaptor> in BMLS
    *** comment: inhibited by addition of 1% NP40 to the lysis buffer
    *** source: 12150926-Fig-3a
  *** xMtor[tAb] copptby[WB] xRaptor[tAb] is detectable
    *** cells: HEK293<xMtor><xRaptor> in BMLS
    *** comment: inhibited by addition of 1% NP50 to the lysis buffer
    *** comment: unaffected by Serum-deprivation and/or AA-deprivation
    *** unaffected by: Rapamycin [addition]
    *** source: 12150926-Fig-2a

High Salt OK

  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293E in BMS
    *** comment: unaffected by washing ppt with high salt
    *** source: 19446321-Fig-1d
  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293E in BMS
    *** comment: unaffected by washing ppt with high salt
    *** source: 19446321-Fig-1d

===============================
Required Raptor characteristics
===============================

  *** xRaptor[tAb] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xRaptor(mnr)"Mtor-binding-mut" [substitution]
    *** source: 15268862-Fig-2c
  *** xRaptor[tAb] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** comment: Coppt works in Chaps-LB but not NP40-LB
    *** source: 17043309-Fig-3c



=========
no extras
=========

  *** Mtor[MS] copptby[WB] Raptor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 16962653-Fig-1a
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: DU145 in BMS
    *** source: 15268862-Fig-2a
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 15268862-Fig-1c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 15268862-Fig-2a
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 15268862-Fig-2a
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 12718876(D)
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 17043309-Fig-S5
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: A549 in BMS
    *** source: 16919458-Fig-1c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 16919458-Fig-1c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 16919458-Fig-1c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: JURKAT<Pten~null> in BMS
    *** source: 16919458-Fig-1c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: LNCAP in BMS
    *** source: 16919458-Fig-1c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: MCF7 in BMS
    *** source: 16919458-Fig-1c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: PC3 in BMS
    *** source: 16919458-Fig-1c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: SHSY5Y in BMS
    *** source: 16919458-Fig-1c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: A549 in BMS
    *** source: 12150925-Fig-3c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: BJAB in BMS
    *** source: 12150925-Fig-3c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 12150925-Fig-3c
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: SKNMC in BMS
    *** source: 12150925-Fig-3c
  *** xRaptor[tAb] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 12150925-Fig-3d
  *** xRaptor[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 12150925-Fig-3e
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 15066126-Fig-2b
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 15467718-Fig-1
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293E in BMLS
    *** source: 17386266-Fig-3f
  *** xRaptor[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293E<xPras40><xMtor> in BMLS
    *** source: 17386266-Fig-3e
  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 15467718-Fig-1
  *** xMtor[tAb] copptby[WB] xRaptor[tAb] is detectable
    *** cells: HEK293<xMtor><xRaptor> in BMS
    *** source: 12408816-Fig-7b
  *** xMtor[tAb] copptby[WB] xRaptor[tAb] is detectable
    *** cells: HEK293E<xRaptor><xPras40><xMtor> in BMLS
    *** source: 17386266-Fig-3e
  *** xMtor[tAb] copptby[WB] xMlst8[tAb] is detectable
    *** cells: HEK293<xMtor><xMlst8> in BMS
    *** source: 12408816-Fig-7b
  *** xMtor[tAb] copptby[WB] xMlst8[tAb] is detectable
    *** cells: HEK293E<xMtor><xMlst8><xDeptor> in BMS
    *** source: 19446321-Fig-1f
  *** xRaptor[tAb] copptby[WB] xMlst8[tAb] is detectable
    *** cells: HEK293<xRaptor><xMlst8> in BMS
    *** source: 12408816-Fig-7c
  *** xMlst8[tAb] copptby[WB] xRaptor[tAb] is detectable
    *** cells: HEK293<xRaptor><xMlst8> in BMS
    *** source: 12408816-Fig-7c
  *** Mlst8[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17510057-Fig-1b
  *** Mtor[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17510057-Fig-1b
  *** Mlst8[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-1b
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-1b

  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: mEFs<Rictor~null> in BMS
    *** source: 18925875-Fig-4a
  *** Raptor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17461779-Fig-3a
