auto

  *** xMtor[tAb]IP IVKA(auto)[32P-ATP] is increased itpo xMlst8
    *** cells: HEK293T in BMS
    *** inhibited by: xMlst8(F320S) [substitution]
    *** inhibited by: xMlst8(G192D) [substitution]
    *** inhibited by: xMlst8(S72D) [substitution]
    *** source: 12718876(D)

Eif4epb1 substrate

  *** xMtor[tAb]IP IVKA(Eif4ebp1)[32P-ATP] is increased itpo xMlst8
    *** cells: HEK293T in BMS
    *** source: 12718876(D)
  *** xMtor[tAb]IP IVKA(Eif4ebp1)[32P-ATP] is increased itpo xRaptor
    *** cells: HEK293T in BMS
    *** comment: xMtor[tAb]IP was "activated" with anti-Mtor after IP
    *** source: 12665511-Fig-9
  *** xMtor[tAb]IP IVKA(Eif4ebp1)[32P-ATP] is increased itpo xRaptor
    *** cells: HEK293T in BMS
    *** inhibited by: rEif4ebp1(F113A) [substitution]
    *** inhibited by: rEif4ebp1(I15A/P16A) [substitution]
    *** source: 12665511-Fig-8
  *** xMtor[tAb]IP IVKA(Eif4ebp1)(T36/T45)[phosAb] is increased itpo xRaptor
    *** cells: HEK293T in BMS
    *** inhibited by: rEif4ebp1(F113A) [substitution]
    *** inhibited by: rEif4ebp1(I15A/P16A) [substitution]
    *** source: 12665511-Fig-8
  *** xMtor[tAb]IP IVKA(Eif4ebp1)(T69)[phosAb] is increased itpo xRaptor
    *** cells: HEK293T in BMS
    *** inhibited by: rEif4ebp1(F113A) [substitution]
    *** unaffected by: rEif4ebp1(I15A/P16A) [substitution]
    *** source: 12665511-Fig-8
  *** xMtor[Ab]IP IVKA(Eif4ebp1)[32P-ATP] is increased itpo xRaptor
    *** cells: HEK293<xMtor> in BMS
    *** comment: cells were lysed without detergent
    *** comment: inhibited by addition of 1% NP50 to the lysis buffer
    *** source: 12150926-Fig-3a
  *** xMtor[Ab]IP IVKA(Eif4ebp1)[32P-ATP] is increased itpo xRaptor
    *** cells: HEK293<xMtor> in BMS
    *** comment: cells were lysed without detergent
    *** comment: inhibited by washing IP with 0.25-1% Chaps
    *** source: 12150926-Fig-3b

S6k1 substrate

  *** xMtor[Ab]IP IVKA(S6k1)[32P-ATP] is increased itpo xRaptor
    *** cells: HEK293<xMtor> in BMS
    *** source: 12150926-Fig-3c
  *** xMtor[tAb]IP IVKA(S6k1)[32P-ATP] is increased itpo xMlst8
    *** cells: HEK293T in BMS
    *** inhibited by: xMlst8(F320S) [substitution]
    *** inhibited by: xMlst8(G192D) [substitution]
    *** inhibited by: xMlst8(S72D) [substitution]
    *** source: 12718876(D)
  *** Mtor[Ab]IP IVKA(S6k1)[32P-ATP] is decreased itpo xRaptor
    *** cells: HEK293T in BMS
    *** inhibited by: xRaptor(Y194A/D195A/C196A) [substitution]
    *** source: 12150925-Fig-6e
  *** Mtor[Ab]IP IVKA(S6k1)(T412)[phosAb] is detectable
    *** cells: HELA in BMS
    *** does not req: Rictor [RNAi]
    *** does not req: Sin1 [RNAi]
    *** source: 17043309-Fig-S6
  *** xMtor[tAb]IP IVKA(S6k1)(T412)[phosAb] is increased itpo xRheb
    *** cells: HEK293T<xMtor> in BMS
    *** inhibited by: xRheb(D60I) [substitution]
    *** source: 15854902-Fig-S5
  *** xMtor[tAb]IP IVKA(S6k1)[32P-ATP] is increased itpo xRheb
    *** cells: HEK293T<xMtor> in BMS
    *** inhibited by: xRheb(D60I) [substitution]
    *** source: 15854902-Fig-S5


increase by Rheb-GTP

  *** Raptor[Ab]IP IVKA(Eif4ebp1)(T37/T46)[phosAb] is increased by xRheb{GTPgS}[tAb]IP
    *** cells: none
    *** inhibited by: xRheb{GDP}[tAb]IP [substitution]
    *** inhibited by: xRap2a{GTPgS}[tAb]IP [substitution]
    *** inhibited by: xRap2a{GDP}[tAb]IP [substitution]
    *** comment: Raptor IP is from mEFs<Tsc2~null> in BMLS
    *** comment: xRheb IP is from HEK293T<xRheb> in BMLS
    *** comment: xRap2a IP is from HEK293T<xRap2a> in BMLS
    *** source: 17386266-Fig-2e
  *** xRaptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased by xRheb{GTPgS}[tAb]IP
    *** cells: none
    *** comment: inhibited by addition of rPras40 to IVKA reaction
    *** comment: inhibition by rPras40 can be reversed by higher concentrations of xRheb{GTPgS}
    *** comment: xRaptor IP is from HEK293E<xRaptor> in BMLS
    *** comment: xRheb IP is from HEK293T<xRheb> in BMLS
    *** source: 17386266-Fig-4e
  *** xRaptor[tAb]IP IVKA(S6k1)(T412)[phosAb] is increased by xRheb{GTPgS}[tAb]IP
    *** cells: none
    *** inhibited by: xRheb{GDP}[tAb]IP [substitution]
    *** inhibited by: xRap2a{GTPgS}[tAb]IP [substitution]
    *** inhibited by: xRap2a{GDP}[tAb]IP [substitution]
    *** comment: xRaptor IP is from HEK293E<xRaptor> in BMLS
    *** comment: xRheb IP is from HEK293T<xRheb> in BMLS
    *** comment: xRap2a IP is from HEK293T<xRap2a> in BMLS
    *** source: 17386266-Fig-2c
  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased by xRheb{GTPgS}[tAb]IP
    *** cells: none
    *** comment: inhibited by addition of rPras40 to IVKA reaction
    *** comment: Raptor IP is from HEK293E in BMLS
    *** comment: xRheb IP is from HEK293T<xRheb> in BMLS
    *** source: 17386266-Fig-4c
  *** xRaptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased by xRheb{GTPgS}[tAb]IP
    *** cells: none
    *** comment: inhibited by addition of rPras40 to IVKA reaction
    *** comment: xRaptor IP is from HEK293E<xRaptor> in BMLS
    *** comment: xRheb IP is from HEK293T<xRheb> in BMLS
    *** source: 17386266-Fig-4d
  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased by xRheb{GTPgS}[tAb]IP
    *** cells: none
    *** inhibited by: xRheb{GDP}[tAb]IP [substitution]
    *** inhibited by: xRap2a{GTPgS}[tAb]IP [substitution]
    *** inhibited by: xRap2a{GDP}[tAb]IP [substitution]
    *** comment: Raptor IP is from HEK293E in BMLS
    *** comment: xRheb IP is from HEK293T<xRheb> in BMLS
    *** comment: xRap2a IP is from HEK293T<xRap2a> in BMLS
    *** source: 17386266-Fig-2a
  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased by xRheb{GTPgS}[tAb]IP
    *** cells: none
    *** inhibited by: xRheb{GDP}[tAb]IP [substitution]
    *** inhibited by: xRap2a{GTPgS}[tAb]IP [substitution]
    *** inhibited by: xRap2a{GDP}[tAb]IP [substitution]
    *** comment: Raptor IP is from HEK293E in BMLS
    *** comment: xRheb IP is from HEK293T<xRheb> in BMLS
    *** comment: xRap2a IP is from HEK293T<xRap2a> in BMLS
    *** source: 17386266-Fig-4c
  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased by xRheb{GTPgS}[tAb]IP
    *** cells: none
    *** inhibited by: xRheb{GDP}[tAb]IP [substitution]
    *** inhibited by: xRap2a{GTPgS}[tAb]IP [substitution]
    *** inhibited by: xRap2a{GDP}[tAb]IP [substitution]
    *** comment: Raptor IP is from mEFs<Tsc2~null> in BMLS
    *** comment: xRheb IP is from HEK293T<xRheb> in BMLS
    *** comment: xRap2a IP is from HEK293T<xRap2a> in BMLS
    *** source: 17386266-Fig-2c
  *** Raptor[Ab]IP IVKA(S6k1)(T412)[phosAb] is increased itpo xRheb
    *** cells: HEK293T in BMLS
    *** source: 18497260-Fig-S7

Rheb-IP

  *** xRheb[tAb]IP IVKA(Eif4ebp1)(T37/T36)[phosAb] is increased itpo xMtor
    *** cells: HEK293T<xRheb> in BMS
    *** inhibited by: xMtor(N2343K)"KD" [substitution]
    *** source: 15854902-Fig-6a
  *** xRheb[tAb]IP IVKA(Eif4ebp1)(T37/T36)[phosAb] is increased itpo xMtor
    *** cells: HEK293T<xRheb> in BMS
    *** inhibited by: xRheb(S20N) [substitution]
    *** inhibited by: xRheb(D60I) [substitution]
    *** source: 15854902-Fig-6a
  *** xRheb[tAb]IP IVKA(Eif4ebp1)[32P-ATP] is increased itpo xMtor
    *** cells: HEK293T<xRheb> in BMS
    *** inhibited by: xMtor(N2343K)"KD" [substitution]
    *** source: 15854902-Fig-6a
  *** xRheb[tAb]IP IVKA(Eif4ebp1)[32P-ATP] is increased itpo xMtor
    *** cells: HEK293T<xRheb> in BMS
    *** inhibited by: xRheb(S20N) [substitution]
    *** inhibited by: xRheb(D60I) [substitution]
    *** source: 15854902-Fig-6a
  *** xRheb[tAb]IP IVKA(S6k1)(T412)[phosAb] is increased itpo xMtor
    *** cells: HEK293T<xRheb> in BMS
    *** enhanced by: xRheb(Q64L) [substitution]
    *** inhibited by: xRheb(D60I) [substitution]
    *** source: 15854902-Fig-6a
  *** xRheb[tAb]IP IVKA(S6k1)(T412)[phosAb] is increased itpo xMtor
    *** cells: HEK293T<xRheb> in BMS
    *** inhibited by: xMtor(N2343K)"KD" [substitution]
    *** source: 15854902-Fig-6a
  *** xRheb[tAb]IP IVKA(S6k1)[32P-ATP] is increased itpo xMtor
    *** cells: HEK293T<xRheb> in BMS
    *** inhibited by: xMtor(N2343K)"KD" [substitution]
    *** source: 15854902-Fig-6a

============================================
Interaction between Mtorc1 proteins and Rheb
============================================

  *** rMlst8[tAb] boundby[WB] rRheb{NucFree}[GST] is detectable
    *** cells: none
    *** unaffected by: rRheb{GDP} [substitution]
    *** unaffected by: rRheb{GMPPNP} [substitution]
    *** source: 15854902-Fig-5a
  *** xMlst8[tAb] copptby[WB] xRheb[tAb] is detectable
    *** cells: HEK293T<xRheb><xMlst8> in BMS
    *** source: 15854902-Fig-4b
  *** xMtor(1967-2191)[tAb] copptby[WB] xRheb[GST] is detectable
    *** cells: HEK293T<xRheb><xMtor(1967-2191)> in BMS
    *** inhibited by: xMtor(1967-2147) [substitution]
    *** unaffected by: xMtor(2148-2549) [substitution]
    *** unaffected by: xMtor(2148-2300) [substitution]
    *** source: 15854902-Fig-3b
  *** xMtor(1967-2191)[tAb] copptby[WB] xRheb[GST] is detectable
    *** cells: HEK293T<xRheb><xMtor(1967-2191)> in BMS
    *** inhibited by: xMtor(1967-2147) [substitution]
    *** unaffected by: xMtor(2148-2549) [substitution]
    *** unaffected by: xMtor(2148-2517) [substitution]
    *** unaffected by: xMtor(2148-2429) [substitution]
    *** unaffected by: xMtor(2148-2300) [substitution]
    *** source: 15854902-Fig-S3b
  *** xMtor(1967-2191)[tAb] copptby[WB] xRheb[GST] is detectable
    *** cells: HEK293T<xRheb><xMtor(1967-2191)> in BMS
    *** unaffected by: xMtor(2148-2549) [substitution]
    *** inhibited by: xMtor(1265-1967) [substitution]
    *** inhibited by: xMtor(916-1643) [substitution]
    *** source: 15854902-Fig-S3a
  *** rMtor(2148-2549)[tAb] boundby[WB] rRheb{NucFree}[GST] is detectable
    *** cells: none
    *** unaffected by: rRheb{GDP} [substitution]
    *** inhibited by: rRheb{GMPPNP} [substitution]
    *** source: 15854902-Fig-5a
  *** xMtor(2148-2549)[tAb] copptby[WB] xRheb[GST] is detectable
    *** cells: HEK293T<xRheb><xMtor(2148-2549)> in BMS
    *** source: 15854902-Fig-4a
  *** Mtor[Ab] copptby[WB] xRheb[GST] is detectable
    *** cells: HEK293T<xRheb> in BMS
    *** enhanced by: xRheb(T38M) [substitution]
    *** partially inhibited by: xRheb(I39K) [substitution]
    *** inhibited by: xRheb(Q64L) [substitution]
    *** unaffected by: xRheb(S20N) [substitution]
    *** source: 15854902-Fig-2b
  *** Mtor[Ab] copptby[WB] xRheb[GST] is detectable
    *** cells: HEK293T<xRheb> in BMS
    *** source: 15854902-Fig-2a
  *** xMtor[tAb] copptby[WB] xRheb[GST] is detectable
    *** cells: HEK293T<xRheb><xMtor> in BMS
    *** source: 15854902-Fig-2c
  *** xMtor[tAb] copptby[WB] xRheb[tAb] is detectable
    *** cells: HEK293T<xRheb><xMtor> in BMS
    *** unaffected by: xRheb(Q64L) [substitution]
    *** unaffected by: xRheb(D60I) [substitution]
    *** source: 15854902-Fig-7b
  *** xRaptor(1009-1335)[tAb] copptby[WB] xRheb[tAb] is detectable
    *** cells: HEK293T<xRheb><xRaptor(1009-1335)> in BMS
    *** inhibited by: xRaptor [substitution]
    *** inhibited by: xRaptor(1-903) [substitution]
    *** inhibited by: xRaptor(904-1335) [substitution]
    *** source: 15854902-Fig-4b
  *** Raptor[Ab] copptby[WB] xRheb[GST] is detectable
    *** cells: HEK293T<xRheb> in BMS
    *** source: 15854902-Fig-2a
  *** rRaptor[tAb] boundby[WB] rRheb{NucFree}[GST] is detectable
    *** cells: none
    *** unaffected by: rRheb{GDP} [substitution]
    *** unaffected by: rRheb{GMPPNP} [substitution]
    *** source: 15854902-Fig-5a
  *** xMtor[tAb] copptby[WB] xRheb[tAb] is detectable
    *** cells: HEK293T<xMtor><xRheb> in BMLS
    *** unaffected by: AA-deprivation (1 hr) [stim]
    *** unaffected by: H2O2 (30 min) [stim]
    *** inhibited by: Sorbitol (30 min) [stim]
    *** unaffected by: Arsenite (30 min) [stim]
    *** source: 15772076-Fig-6a
  *** xMtor[tAb] copptby[WB] xRheb[tAb] is detectable
    *** cells: HEK293T<xMtor><xRheb> in BMLS
    *** unaffected by: xRheb(D60V) [substitution]
    *** unaffected by: xRheb(D60K) [substitution]
    *** source: 15772076-Fig-6b
