=======================
direct phos by Raf1-act
=======================

  *** rMek1(K97M)[32P] phos[32P-ATP] is increased by xRaf1(del(66-303))[tAb]IP
    *** cells: none
    *** IPfrom: HEK293 in BMS
    *** source: 10232608-Fig-5a

  *** rMek1[32P] phos[32P-ATP] is unchanged by xRaf1[tAb]IP
    *** cells: none
    *** IPfrom: SF9<xHras(mnr)"CA"><xSrc> in BMS
    *** comment: rErk1(mnr)"KD" was included in the reaction mix
    *** source: 12738796-Fig-2a

=======================
Semi Direct Activation
=======================

  *** rErk1 IVKA(MBP)[32P-ATP] is increased by xRaf1[tAb]IP
    *** cells: none
    *** comment: xRaf1-IP was from Sf9 cells cotransfected with xHras-act + xSrc
    *** comment: rMek1 was included in the reaction mix
    *** unaffected by: rMlk3 [addition]
    *** unaffected by: rMlk3(K144A)"KD" [addition]
    *** source: 12738796-Fig-2b
  *** rErk1(mnr)"KD"[32P] phos[32P-ATP] is increased by (rMek1 + xRaf1[tAb]IP)
    *** cells: none
    *** comment: xRaf1-IP was from Sf9 cells cotransfected with xHras-act + xSrc
    *** reqs: both [omissions]
    *** unaffected by: rMlk3 [addition]
    *** unaffected by: rMlk3(K144A)"KD" [addition]
    *** source: 12738796-Fig-2a
  *** rErk1(K52R)[32P] phos[32P-ATP] is increased by xRaf1(del(66-303))[tAb]IP
    *** cells: none
    *** comment: rMek1 is present in the reaction mix
    *** comment: xRaf1(del(66-303)) IP is from HEK293 cells
    *** source: 10232608-Fig-5c

====
IVKA
====

  *** xMek1[tAb]IP IVKA(Erk1)[32P-ATP] is increased itpo xRaf1(del(66-303))"CA"
    *** cells: NIH3T3 in BMLS
    *** source: 9006902-Fig-3b
  *** xMek1[Ab]IP IVKA(Erk2)[32P-ATP] is increased itpo xRaf1(mnr)"CA"
    *** cells: REF52 in BMS
    *** source: 10480934-Fig-5d
  *** xMek1[Ab]IP IVKA(Erk2)[32P-ATP] is increased itpo xRaf1
    *** cells: REF52 in BMS
    *** source: 10480934-Fig-5d

  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is unchanged itpo xRaf1
    *** cells: HEK293 in BMLS
    *** source: 9351825-Fig-5a
  *** xMek2[tAb]IP IVKA(Erk2)[32P-ATP] is unchanged itpo xRaf1
    *** cells: HEK293 in BMLS
    *** source: 9351825-Fig-5b

  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased itpo xRaf1(del(66-303))"CA"
    *** cells: HELA in BMS
    *** inhibited by: xBrap [addition]
    *** source: 14724641-Fig-2a

===============
phos(S218/S222)  does not req Pak1
===============

  *** rMek1[32P] phos(S218/S222)[HPLC-PAA-ED] is increased by rRaf1(del(66-303))"CA"
    *** cells: none
    *** source: 8157000(D)
  ------------------------------------------------------------------------------
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased itpo xRaf1
    *** cells: COS7 in BMLS
    *** inhibited by: xRaf1(S471A) [substitution]
    *** unaffected by: xRaf1(S481A) [substitution]
    *** source: 16093354-Fig-7a
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased itpo xRaf1(del(66-303))"CA"
    *** cells: COS7 in BMLS
    *** inhibited by: xRaf1(del(66-303)/S471A) [substitution]
    *** source: 16093354-Fig-8b
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased itpo xRaf1(S259D)"CA"
    *** cells: HEK293 in BMLS
    *** unaffected by: xMek1(T292A) [substitution]
    *** unaffected by: xMek1(S298A) [substitution]
    *** unaffected by: xMek1(T292A/S298A) [substitution]
    *** source: 11948406-Fig-6a
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased itpo xRaf1(S259D)"CA"
    *** cells: HEK293 in BMLS
    *** unaffected by: xPak1((165-544)/K298A)"DN" [addition]
    *** source: 11948406-Fig-2a
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased itpo xRaf1(S259D)"CA"
    *** cells: HEK293<xPak1((165-544)/K298A)> in BMLS
    *** inhibited by: xRaf1(WT) [substitution]
    *** inhibited by: xRaf1(S338A/S339C) [substitution]
    *** inhibited by: xRaf1(S338D/S339E) [substitution]
    *** source: 11948406-Fig-2a
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased itpo xRaf1(S259D)"CA"
    *** cells: HEK293<xPak1((165-544)/K298A)> in BMLS
    *** inhibited by: xRaf1(WT) [substitution]
    *** unaffected by: xRaf1(S259D/S338A/S339A) [substitution]
    *** unaffected by: xRaf1(S259D/S338D/S339E) [substitution]
    *** inhibited by: xRaf1(S338D/S339E) [substitution]
    *** source: 11948406-Fig-2b
  ------------------------------------------------------------------------------
  *** xMek1(T292A)[tAb]IP phos(S218/S222)[phosAb] is increased itpo xRaf1
    *** cells: HEK293<xPak1(165-544)"CA"> in BMLS
    *** source: 11948406-Fig-4c


==========
phos(T292)
==========

  *** xMek1[tAb]IP phos(T292)[phosAb] is increased itpo xRaf1(mnr)"CAAX"
    *** cells: COS1 in BMLS
    *** enhanced by: xErk2 [addition]
    *** source: 14993270-Fig-4b,6a
  *** xMek1[tAb]IP phos(T292)[phosAb] is increased itpo xRaf1(mnr)"CAAX"
    *** cells: COS1 in BMLS
    *** source: 14993270-Fig-4b
  *** xMek1[tAb]IP phos(T292)[phosAb] is increased itpo xRaf1
    *** cells: HEK293 in BMLS
    *** source: 11948406-Fig-4b
  -----------------------------------------------------------------------
  *** xMek1(S298A)[tAb]IP phos(T292)[phosAb] is increased itpo xRaf1
    *** cells: HEK293<xPak1(165-544)"CA"> in BMLS
    *** source: 11948406-Fig-4c

==========
phos(S298)
===========

  *** xMek1[tAb]IP phos(S298)[phosAb] is unchanged itpo xRaf1(mnr)"CAAX"
    *** cells: COS1 in BMLS
    *** source: 14993270-Fig-3a,6a
  *** xMek1[tAb]IP phos(S298)[phosAb] is decreased itpo (xRaf1(mnr)"CAAX" + xErk2)
    *** cells: COS1 in BMLS
    *** reqs: both [omissions]
    *** source: 14993270-Fig-3a,6a
  *** xMek1[tAb]IP phos(S298)[phosAb] is unchanged itpo xRaf1
    *** cells: HEK293 in BMLS
    *** source: 11948406-Fig-4a
  -----------------------------------------------------------------------
  *** Mek1[phosAb] phos(S298)[phosAb] is detectable
    *** cells: rEFs in BMLS
    *** does not req: Raf1 [RNAi]
    *** source: 17314031-Fig-3

==========
Raf1 coppt
==========

  *** xMek1[Ab] copptby[WB] xRaf1[tAb] is detectable
    *** cells: COS in BMS
    *** source: 8120027-Fig-4
  *** xMek1[tAb] copptby[WB] xRaf1[tAb] is detectable
    *** cells: COS1 in BMS
    *** partially inhibited by: xRaf1(S259A) [substitution]
    *** source: 11782426-Fig-4c,5
  *** xRaf1[tAb] copptby[WB] xMek1[tAb] is detectable
    *** cells: HEK293 in BMLS
    *** unaffected by: xRac2(G12V)"CA" [addition]
    *** unaffected by: xMek1(T292A) [substitution]
    *** partially inhibited by: xMek1(S298A) [substitution]
    *** source: 9351825-Fig-7c
  *** xRaf1[tAb] copptby[WB] xMek1[tAb] is detectable
    *** cells: COS7 in BMS
    *** inhibited by: xRaf1(S471A) [substitution]
    *** inhibited by: xRaf1(S471C) [substitution]
    *** inhibited by: xRaf1(S471D) [substitution]
    *** unaffected by: xRaf1(S481A) [substitution]
    *** source: 16093354-Fig-10c
  *** xRaf1[tAb] copptby[WB] xMek1[tAb] is detectable
    *** cells: COS1 in BMS
    *** partially inhibited by: xRaf1(S259A) [substitution]
    *** partially inhibited by: xRaf1(S259D) [substitution]
    *** source: 11782426-Fig-4c
  *** xRaf1[Ab] copptby[WB] xMek1[tAb] is detectable
    *** cells: HEK293 in BMLS
    *** unaffected by: xMek1(S298A) [substitution]
    *** source: 11948406-Fig-5a
  *** xRaf1(S259D)[Ab] copptby[WB] xMek1[tAb] is detectable
    *** cells: HEK293<xMek1> in BMS
    *** enhanced by: xMek1(mnr)"CA-del(265-301)" [substitution]
    *** source: 9677429-Fig-5c
  *** Raf1[Ab] copptby[WB] xMek1(mnr)"CA-del(265-301)"[tAb] is detectable-but unchanged irt Egf (times)
    *** cells: HEK293<xMek1(mnr)"CA-del(265-301)"> in BMS
    *** times: 0+ 2+ 5+ 10+ min
    *** source: 9677429-Fig-5c
  *** Raf1[Ab] copptby[WB] xMek1(mnr)"CA"[tAb] is detectable
    *** cells: HEK293<xMek1(mnr)"CA"> in BMS
    *** enhanced by: xMek1(mnr)"CA-del(265-301)" [substitution]
    *** source: 9677429-Fig-5b
  *** Raf1[Ab] copptby[WB] xMek1[tAb] is detectable
    *** cells: HEK293<xMek1> in BMS
    *** enhanced by: xMek1(mnr)"CA-del(265-301)" [substitution]
    *** source: 9677429-Fig-5c

  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased itpo xRaf1(305-648)"CA"
    *** cells: H197 in BMS
    *** inhibited by: Rottlerin [pretreatment]
    *** inhibited by: Chelerythrine [pretreatment]
    *** unaffected by: Go6976 [pretreatment]
    *** unaffected by: PD98059 [pretreatment]
    *** source: 10330161-Fig-9b



