========================================
direct phosphorylation by activated Mlk3
========================================

  *** rMek1[32P] phos[32P-ATP] is increased by rMlk3
    *** cells: none
    *** comment: rErk1(mnr)"KD" was included in the reaction mix
    *** inhibited by: rMlk3(K144A)"KD" [substitution]
    *** source: 12738796-Fig-2b
  *** rMek1[phosAb] phos(sitenr)[phosAb] is increased by rMlk3
    *** cells: none
    *** comment: rErk1(mnr)"KD" was included in the reaction mix
    *** source: 12738796-Fig-2c
  *** rMek1[32P] phos[32P-ATP] is detectable
    *** cells: none
    *** comment: rErk1(mnr)"KD" was included in the reaction mix
    *** inhibited by: rMlk3(K144A)"KD" [addition]
    *** unaffected by: rMlk3 [addition]
    *** source: 12738796-Fig-2a

=============================================
semi-direct phosphorylation by activated Mlk3
=============================================

  *** rMek1(K97M)[32P] phos[32P-ATP] is increased by xMlk3[tAb]IP
    *** cells: none
    *** comment: xMlk3 IP is from HEK293 cells
    *** inhibited by: xMlk3(K144A)"KD" [substitution]
    *** source: 10232608-Fig-5a

=============================================
Phosphorylation requires Mlk3 kinase activity
=============================================

  *** xMek1[tAb]IP phos(sitenr)[phosAb] is increased itpo xMlk3
    *** cells: COS7 in BMLS
    *** inhibited by: xMlk3(K144A)"KD" [substitution]
    *** inhibited by: CEP11004 [addition]
    *** source: 12738796-Fig-4c
  *** xMek1[tAb]IP phos(sitenr)[phosAb] is increased itpo xMlk3
    *** cells: COS7 in BMLS
    *** unaffected by: Egf (10 min) [stim]
    *** inhibited by: xMlk3(K144A)"KD" [substitution]
    *** source: 12738796-Fig-2d


