=================================================
inhibited by Tp53 acetyl on Cbp/Ep300 sites
=================================================

  *** rTp53[35S] boundby[WB] rMdm2[GST] is detectable
    *** cells: none
    *** comment: inhibited by substitution with xTp53{acetyl(K164/K120/K372/K373/K381/K382)}[Ab]IP
    *** source: 18485870-Fig-5d
  *** rTp53[Ab] oligo-binding[EMSA] is detectable
    *** cells: none
    *** comment: band is shifted itpo rMdm2 [addition]
    *** comment: shift (Mdm2 binding) is inhibited by substitution with xTp53{acetyl(K164/K120/K372/K373/K381/K382)}[Ab]IP
    *** comment: shift (Mdm2 binding) is unaffected by substitution with xTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR"
    *** source: 18485870-Fig-5b,5c

does not require acetyl on Cbp/Ep300 sites

  *** rMdm2[Ab] boundby[WB] rTp53[GST] is detectable
    *** cells: none
    *** inhibited by: rTp53(L14Q/F19S) [substitution]
    *** inhibited by: rTp53(L22Q/W23S) [substitution]
    *** unaffected by: rTp53(R249A) [substitution]
    *** unaffected by: rTp53(R249S) [substitution]
    *** source: 9878046-Fig-2c
  *** rMdm2[35S] boundby[WB] rTp53[Ab] is detectable
    *** cells: none
    *** unaffected by: xTp53(K370R) [substitution]
    *** unaffected by: xTp53(K372R/K373R) [substitution]
    *** unaffected by: xTp53(K381R/K382R) [substitution]
    *** unaffected by: xTp53(K386R) [substitution]
    *** unaffected by: xTp53(K370R/K372R/K373R) [substitution]
    *** unaffected by: xTp53(K381R/K382R/K386R) [substitution]
    *** unaffected by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 11046142-Fig-5a
  *** rTp53[35S] boundby[WB] rMdm2[GST] is detectable
    *** cells: none
    *** unaffected by: xTp53(K370R) [substitution]
    *** unaffected by: xTp53(K372R/K373R) [substitution]
    *** unaffected by: xTp53(K381R/K382R) [substitution]
    *** unaffected by: xTp53(K386R) [substitution]
    *** unaffected by: xTp53(K370R/K372R/K373R) [substitution]
    *** unaffected by: xTp53(K381R/K382R/K386R) [substitution]
    *** unaffected by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 11046142-Fig-5a
  *** rTp53[35S] boundby[WB] rMdm2[GST] is detectable
    *** cells: none
    *** unaffected by: rTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-S4
  *** xTp53[Ab] copptby[WB] xMdm2[Ab] is detectable
    *** cells: U2OS<xTp53><xMdm2> in BMS
    *** unaffected by: xTp53(K370I/K372I/K373I/K381I/K382I/K386I) [substitution]
    *** unaffected by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 11713287-Fig-3a
  *** xMdm2[Ab] copptby[WB] xTp53[tAb] is detectable
    *** cells: H1299<Tp53~null><xTp53><xMdm2> in BMS
    *** unaffected by: rTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-S11

===========================
Mdm2 binding sites on Tp53
===========================

  *** rMdm2[35S] boundby[WB] rTp53(1-73)[GST] is detectable
    *** cells: none
    *** unaffected by: rTp53(100-290) [substitution]
    *** unaffected by: rTp53(290-393) [substitution]
    *** source: 18485870-Fig-5d
  *** rMdm2[Ab] boundby[WB] rTp53[GST] is detectable
    *** cells: none
    *** partially inhibited by: rTp53(1-369) [substitution]
    *** partially inhibited by: rTp53(1-350) [substitution]
    *** inhibited by: rTp53(1-326) [substitution]
    *** inhibited by: rTp53(1-289) [substitution]
    *** partially inhibited by: rTp53(1-160) [substitution]
    *** source: 9878046-Fig-2a
  *** xTp53[Ab] copptby[WB] xMdm2[Ab] is detectable
    *** cells: U2OS<xTp53><xMdm2> in BMS
    *** inhibited by: xTp53(mnr)"Mdm2-binding-mut" [substitution]
    *** source: 11713287-Fig-3a
  *** xMdm2[Ab] copptby[WB] xTp53[tAb] is detectable
    *** cells: H1299<Tp53~null><xTp53><xMdm2> in BMS
    *** inhibited by: xTp53(L14Q/F19S) [substitution]
    *** inhibited by: xTp53(20-393) [substitution]
    *** inhibited by: xTp53(L14Q/F19S/K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** inhibited by: xTp53(20-393/K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 18485870-Fig-S11

===========================
Tp53 binding sites on Mdm2
===========================


===================================
Effects of Tp53 phosphorylation ???
===================================

Inhibited by phos by Dnapk--  ???

  *** rMdm2[35S] boundby[WB] rTp53[GST] is detectable
    *** cells: none
    *** comment: unaffected by Tp53 pre-phos with pDnapk
    *** source: 10606744-Fig-3b
  *** rTp53(S15A)[Ab] boundby[WB] xMdm2[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** comment: partially inhibited when rTp53 is pre-phosed with partially-purified Dnapk
    *** source: 9363941-Fig-4b
  *** rTp53(S15A/S37A)[Ab] boundby[WB] xMdm2[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** comment: unchanged when rTp53 is pre-phosed with partially-purified Dnapk
    *** source: 9363941-Fig-4b
  *** rTp53(S37A)[Ab] boundby[WB] xMdm2[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** comment: partially inhibited when rTp53 is pre-phosed with partially-purified Dnapk
    *** source: 9363941-Fig-4b

Inhibited by phos by Csnk1d--  ???

  *** rMdm2[35S] boundby[WB] rTp53[GST] is detectable
    *** cells: none
    *** comment: unaffected by Tp53 pre-phos with pDnapk
    *** comment: unaffected by Tp53 pre-phos with rCsnk1d
    *** comment: partially inhibited by pre-phos with pDnapk followed by rCsnk1d
    *** source: 10606744-Fig-3b

====================================
Effects of Mdm2 phosphorylation ???
====================================

Cdk2--

  *** rTp53[Ab] boundby[WB] rMdm2[GST] is detectable
    *** cells: none
    *** comment: inhibited by Mdm2 phos by (xCyclinA2[Ab]IP + xCdk2[Ab]IP)
    *** source: 11359766-Fig-7c


  *** rTp53[Ab] boundby[WB] xMdm2[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** comment: decreased when rTp53 is pre-phosed with partially-purified Dnapk
    *** source: 9363941-Fig-4b

  *** xMdm2[Ab]IP boundby[WB] rTp53[Ab] is detectable
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** comment: decreased when rTp53 is pre-phosed with partially-purified Dnapk
    *** source: 9363941-Fig-4a



==========================
Mdm2 binds to DNA via Tp53
==========================

  *** rMdm2[Ab] oligo-binding[EMSA] is increased by rTp53
    *** cells: none
    *** unaffected by: rTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-5b,5c
  *** rMdm2[Ab] oligo-binding[EMSA] is increased by rTp53
    *** cells: none
    *** unaffected by: rTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-S11







