=======================================================
Lkb1 kinase activity is increased itpo Strada and Cab39
=======================================================

  *** xLkb1[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xStrada
    *** cells: HEK293T in BMS
    *** source: 12805220-Fig-4a

requires Cab39 not Cab39L

  *** xLkb1[GST]IP IVKA(auto)[32P-ATP] is increased itpo xStrada
    *** cells: HEK293 in BMS
    *** enhanced by: xCab39 [addition]
    *** unaffected by: xCab39L [addition]
    *** source: 14517248-Fig-9
  *** xLkb1[GST]IP IVKA(MBP)[32P-ATP] is increased itpo xStrada
    *** cells: HEK293 in BMS
    *** enhanced by: xCab39 [addition]
    *** enhanced by: xCab39L [addition]
    *** source: 14517248-Fig-9
  *** xLkb1[GST]IP IVKA(MBP)(T65)[phosAb] is increased itpo xStrada
    *** cells: HEK293 in BMS
    *** enhanced by: xCab39 [addition]
    *** enhanced by: xCab39L [addition]
    *** source: 14517248-Fig-9
  *** Lkb1[GST]IP IVKA(MBP)(T65)[phosAb] is detectable
    *** cells: HEK293 in BMS
    *** reqs: Cab39 [RNAi]
    *** source: 14517248-Fig-10b
  --- controls ---------------------------------------------------------
  *** xLkb1[GST]IP IVKA(auto)[32P-ATP] is unchanged itpo xCab39
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-9
  *** xLkb1[GST]IP IVKA(MBP)[32P-ATP] is unchanged itpo xCab39
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-9
  *** xLkb1[GST]IP IVKA(MBP)(T65)[phosAb] is unchanged itpo xCab39
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-9

===========================================
Lkb1 autophos sites are T402 T185 T336 T363
===========================================

  *** xLkb1[tAb]IP IVKA(auto)[32P-ATP] is increased itpo xStrada
    *** cells: HEK293T in BMS
    *** comment: autophos sites are T402 T185 T336 T363 by HPLC-PAA-ED
    *** source: 12805220-Fig-5a

======================================================================
Lkb1 total phos is increased itpo Strada
requires Lkb1 kinase activity but not Lkb1(303-306) (Strada binding)
======================================================================

  *** xLkb1[tAb]IP phos[32P-ATP] is increased itpo xStrada
    *** cells: HEK293T in BMS
    *** inhibited by: xLkb1(D176Y)"KD" [substitution]
    *** source: 12805220-Fig-3a
  *** xLkb1[tAb]IP phos[32P-ATP] is increased itpo xStrada
    *** cells: HEK293T in BMS
    *** unaffected by: xLkb1(del(303-305)/H306N) [substitution]
    *** source: 12805220-Fig-3b

===============
Lkb1 phos(T336)
===============

  *** xLkb1[phosAb] phos(T336)[phosAb] is increased itpo xStrada
    *** cells: HEK293T in BMS
    *** source: 12805220-Fig-4a

===============
Lkb1 phos(T363)
===============

  *** xLkb1[phosAb] phos(T363)[phosAb] is increased itpo xStrada
    *** cells: HEK293T in BMS
    *** source: 12805220-Fig-4a

=================
Lkb1/Strada coppt
=================

  *** Strada[Ab] copptby[WB] Lkb1[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 12805220-Fig-2a
  *** Strada[Ab] copptby[WB] Lkb1[Ab] is detectable
    *** cells: RAT2 in BMS
    *** source: 12805220-Fig-2a
  *** Strada[Ab] copptby[WB] xLkb1[GST] is detectable
    *** cells: HEK293T in BMS
    *** source: 12805220-Fig-2a
  *** Strada[Ab] copptby[WB] Lkb1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-3a
  *** Strada[Ab] copptby[WB] Lkb1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: Forskolin [addition]
    *** unaffected by: PMA [addition]
    *** source: 14517248-Fig-S2b
  *** Strada[Ab] copptby[WB] Lkb1[Ab] is detectable
    *** cells: RAT2 in BMS
    *** source: 14517248-Fig-3a

requires Cab39

  *** Strada[Ab] copptby[WB] Lkb1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** reqs: Cab39 [RNAi]
    *** source: 14517248-Fig-10b
  *** xStrada[tAb] copptby[WB] xLkb1[GST] is detectable
    *** cells: HEK293 in BMS
    *** enhanced by: xCab39 [addition]
    *** source: 14517248-Fig-8a
  *** xStrada[tAb] copptby[WB] xLkb1[GST] is increased itpo xCab39
    *** cells: HEK293 in BMS
    *** unaffected by: xLkb1(D194A)"KD" [substitution]
    *** source: 14517248-Fig-S4
  *** xStrada[tAb] copptby[WB] xLkb1[GST] is increased itpo xCab39
    *** cells: HEK293 in BMS
    *** unaffected by: xLkb1(S428A) [substitution]
    *** unaffected by: xLkb1(S428D) [substitution]
    *** unaffected by: xLkb1(C430A) [substitution]
    *** source: 14517248-Fig-S2a

requires Lkb1(303-306)

  *** xLkb1[tAb] copptby[WB] xStrada[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xLkb1(del(303-305)/H306N) [substitution]
    *** source: 12805220-Fig-3b
  *** xStrada[Ab] copptby[WB] xLkb1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xLkb1(del(303-305)/H306N) [substitution]
    *** source: 12805220-Fig-3b
  *** xStrada[tAb] copptby[WB] xLkb1[GST] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xLkb1(del(303-305)/H306N) [substitution]
    *** source: 14517248-Fig-S4
  *** xLkb1[tAb] copptby[WB] Strada[Ab] is detectable
    *** cells: A549 in BMS
    *** inhibited by: xLkb1(del(303-305)/H306N) [substitution]
    *** source: 19414597-Fig-7a

does not require Lkb1 kinase activity

  *** xLkb1[tAb] copptby[WB] xStrada[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xLkb1(D176Y)"KD" [substitution]
    *** source: 12805220-Fig-3a
  *** xStrada[Ab] copptby[WB] xLkb1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xLkb1(D176Y)"KD" [substitution]
    *** source: 12805220-Fig-3a
  *** xLkb1[tAb] copptby[WB] Strada[Ab] is detectable
    *** cells: A549 in BMS
    *** unaffected by: xLkb1(D194A)"KD" [substitution]
    *** source: 19414597-Fig-7a

does not require Lkb1 phos(S307)

  *** xLkb1[tAb] copptby[WB] Strada[Ab] is detectable
    *** cells: A549 in BMS
    *** unaffected by: xLkb1(S307A) [substitution]
    *** source: 19414597-Fig-7a


does not require Lkb1 phos(S428)

  *** xStrada[tAb] copptby[WB] xLkb1[GST] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xLkb1(S428A) [substitution]
    *** unaffected by: xLkb1(S428D) [substitution]
    *** source: 14517248-Fig-S2a
  *** xLkb1[tAb] copptby[WB] Strada[Ab] is detectable
    *** cells: A549 in BMS
    *** unaffected by: xLkb1(S428A) [substitution]
    *** source: 19414597-Fig-7a

does not require C430 prenylation

  *** xStrada[tAb] copptby[WB] xLkb1[GST] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xLkb1(C430A) [substitution]
    *** source: 14517248-Fig-S2a

does not require Strada phosphorylation

  *** xStrada[Ab] copptby[WB] xLkb1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xStrada(T329A) [substitution]
    *** unaffected by: xStrada(T329D) [substitution]
    *** unaffected by: xStrada(T419A) [substitution]
    *** unaffected by: xStrada(T419D) [substitution]
    *** unaffected by: xStrada(T329A/T419A) [substitution]
    *** unaffected by: xStrada(T329D/T419D) [substitution]
    *** source: 12805220-Fig-6b

================
Lkb1/Cab39 coppt
================

  *** Cab39[Ab] copptby[WB] Lkb1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-3a
  *** Cab39[Ab] copptby[WB] Lkb1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: Forskolin [addition]
    *** unaffected by: PMA [addition]
    *** source: 14517248-Fig-S2b
  *** Cab39[Ab] copptby[WB] Lkb1[Ab] is detectable
    *** cells: RAT2 in BMS
    *** source: 14517248-Fig-3a
  *** Lkb1[Ab] copptby[WB] Cab39[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-3b
  *** Lkb1[Ab] copptby[WB] Cab39[Ab] is detectable
    *** cells: RAT2 in BMS
    *** source: 14517248-Fig-3b

requires Strada

  *** Cab39[MS] copptby[WB] xLkb1[GST] is increased itpo xStrada
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-4b
  *** xCab39[tAb] copptby[WB] xLkb1[GST] is detectable
    *** cells: HEK293<xStrada> in BMS
    *** source: 14517248-Fig-4c
  *** xCab39[tAb] copptby[WB] xLkb1[GST] is detectable
    *** cells: HEK293<xStradb> in BMS
    *** source: 14517248-Fig-4c
  *** xCab39[tAb] copptby[WB] xLkb1[GST] is undetectable
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-4a

==================
Strada/Cab39 coppt
==================

  *** Strada[Ab] copptby[WB] Cab39[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-3b
  *** Strada[Ab] copptby[WB] Cab39[Ab] is detectable
    *** cells: RAT2 in BMS
    *** source: 14517248-Fig-3b
  *** xCab39[tAb] copptby[WB] xStrada[GST] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xStrada(1-438) [substitution]
    *** inhibited by: xStrada(W429F) [substitution]
    *** source: 14517248-Fig-6a
  *** xCab39[tAb] copptby[WB] xStrada[GST] is detectable
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-4a
  *** xCab39[tAb] copptby[WB] xStradb[GST] is detectable
    *** cells: HEK293 in BMS
    *** source: 14517248-Fig-4a

===================
Strada phos by Lkb1
===================

  *** rStrada[32P] phos[32P-ATP] is increased by rLkb1
    *** cells: none
    *** inhibited by: rLkb1(D176Y)"KD" [substitution]
    *** source: 12805220-Fig-3c

requires Cab39

  *** xStrada[32P] phos[32P-ATP] is increased itpo xCab39
    *** cells: HEK293<xLkb1> in BMS
    *** inhibited by: xStradb [substitution]
    *** source: 14517248-Fig-9
  *** xStrada[32P] phos[32P-ATP] is increased itpo xCab39
    *** cells: HEK293<xLkb1> in BMS
    *** partially inhibited by: xCab39L [substitution]
    *** source: 14517248-Fig-9

requires both Lkb1 kinase activity and Lkb1(303-306)

  *** xStrada[tAb]IP phos[32P-ATP] is increased by xLkb1[tAb]IP
    *** cells: none
    *** IPfrom: HEK293T in BMS
    *** inhibited by: xLkb1(del(303-305)/H306N)[tAb]IP [substitution]
    *** inhibited by: xLkb1(D176Y)"KD"[tAb]IP [substitution]
    *** source: 12805220-Fig-3c
  *** xStrada[tAb]IP phos[32P-ATP] is increased itpo xLkb1
    *** cells: HEK293T in BMS
    *** inhibited by: xLkb1(D176Y)"KD" [substitution]
    *** source: 12805220-Fig-3a
  *** xStrada[tAb]IP phos[32P-ATP] is increased itpo xLkb1
    *** cells: HEK293T in BMS
    *** unaffected by: xLkb1(del(303-305)/H306N) [substitution]
    *** source: 12805220-Fig-3b

===============================
Strada phos sites are T392 T419
===============================

  *** xStrada[tAb]IP phos[32P-ATP] is detectable
    *** cells: none
    *** IPfrom: HEK293T<xLkb1> in BMS
    *** comment: phos sites are T392 T419 by HPLC-PAA-ED
    *** source: 12805220-Fig-6a
  *** xStrada[tAb]IP phos[32P-ATP] is detectable
    *** cells: none
    *** IPfrom: HEK293T<xLkb1> in BMS
    *** unaffected by: xStrada(T329A)[tAb]IP [substitution]
    *** partially inhibited by: xStrada(T329D)[tAb]IP [substitution]
    *** unaffected by: xStrada(T419A)[tAb]IP [substitution]
    *** unaffected by: xStrada(T419D)[tAb]IP [substitution]
    *** inhibited by: xStrada(T329A/T419A)[tAb]IP [substitution]
    *** inhibited by: xStrada(T329D/T419D)[tAb]IP [substitution]
    *** source: 12805220-Fig-6b
