=====================
Phosphorylation Sites
=====================

Total phos

  *** rEif4ebp1[32P] phos[32P-ATP] is unchanged by xMtor[tAb]IP
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** source: 10200280-Fig-4a
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  *** rEif4ebp1[32P] phos[32P-ATP] is increased by xMtor[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xMtor> in BMS
    *** inhibited by: xMtor(D2357E)"KD" [substitution]
    *** source: 9465032-Fig-1a
  *** rEif4ebp1[32P] phos[32P-ATP] is increased by xMtor[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xMtor> in BMS
    *** inhibited by: xMtor(D2357E)"KD" [substitution]
    *** source: 9465032-Fig-4a
  *** rEif4ebp1[32P] phos[32P-ATP] is increased by xMtor[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xMtor> in BMS
    *** partially inhibited by: rFkbp12 + Rapamycin [addition]
    *** source: 9465032-Fig-1b
  *** rEif4ebp1[32P] phos[32P-ATP] is increased by xMtor[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xMtor> in BMS
    *** unaffected by: rEif4ebp1(T35A/T36A) [substitution]
    *** unaffected by: rEif4ebp1(T44A/T46A) [substitution]
    *** inhibited by: rEif4ebp1(T36A/T45A) [substitution]
    *** inhibited by: rEif4ebp1(T35A/T36A/T44A/T45A) [substitution]
    *** unaffected by: rEif4ebp1(S64A) [substitution]
    *** unaffected by: rEif4ebp1(T69A) [substitution]
    *** source: 9465032-Fig-4b
  *** rEif4ebp1[32P] phos[32P-ATP] is increased by xMtor[tAb]IP
    *** cells: none
    *** comment: xMtor[tAb]IP from HEK293 in BMS
    *** comment: increase is over xMtor(D2357E)"KD"[tAb]IP
    *** source: 9405468-Fig-1a
  *** rEif4ebp1[32P] phos[32P-ATP] is increased by (xMtor[tAb]IP + anti-Mtor)
    *** cells: none
    *** comment: xMtor[tAb]IP from HEK293T in BMS
    *** reqs: both [omissions]
    *** unaffected by: Rapamycin [addition]
    *** unaffected by: rFkbp12 [addition]
    *** partially inhibited by: Rapamycin + rFkbp12 [addition]
    *** inhibited by: LY294002 [addition]
    *** source: 12665511-Fig-4
  *** rEif4ebp1[32P] phos[32P-ATP] is increased by xMtor[tAb]IP
    *** cells: none
    *** comment: xMtor[tAb]IP from HEK293T in BMS
    *** comment: xMtor[tAb]IP was "activated" with anti-Mtor after IP
    *** inhibited by: rEif4ebp1(F113A) [substitution]
    *** inhibited by: rEif4ebp1(I15A/P16A) [substitution]
    *** source: 12665511-Fig-2
  *** rEif4ebp1[32P] phos[32P-ATP] is increased by xMtor[tAb]IP
    *** cells: none
    *** comment: xMtor[tAb]IP from HEK293 in BMS
    *** comment: increase is over xMtor(D2357E)"KD"[tAb]IP
    *** unaffected by: rEif4ebp1(S64A) [substitution]
    *** unaffected by: rEif4ebp1(T69A) [substitution]
    *** unaffected by: rEif4ebp1(T35A/T36A) [substitution]
    *** unaffected by: rEif4ebp1(T44A/T45A) [substitution]
    *** inhibited by: rEif4ebp1(T36A/T45A) [substitution]
    *** inhibited by: rEif4ebp1(T35A/T36A/T44A/T45A) [substitution]
    *** source: 9405468-Fig-4b
  *** rEif4ebp1[32P] phos[32P-ATP] is increased by Mtor[Ab]IP
    *** cells: none
    *** comment: Mtor[Ab]IP is from rat brain
    *** comment: phos-sites were shown to be T37 and T46 by MS
    *** source: 10364159-Fig-1,3

phos(T36/T45)

  *** rEif4ebp1[phosAb] phos(T36/T45)[phosAb] is increased by (xMtor[tAb]IP + anti-Mtor)
    *** cells: none
    *** comment: xMtor[tAb]IP from HEK293T in BMS
    *** reqs: both [omissions]
    *** unaffected by: Rapamycin [addition]
    *** unaffected by: rFkbp12 [addition]
    *** partially inhibited by: Rapamycin + rFkbp12 [addition]
    *** inhibited by: LY294002 [addition]
    *** source: 12665511-Fig-4
  *** rEif4ebp1[phosAb] phos(T36/T45)[phosAb] is increased by xMtor[tAb]IP
    *** cells: none
    *** comment: xMtor[tAb]IP from HEK293T in BMS
    *** comment: xMtor[tAb]IP was "activated" with anti-Mtor after IP
    *** inhibited by: rEif4ebp1(F113A) [substitution]
    *** inhibited by: rEif4ebp1(I15A/P16A) [substitution]
    *** source: 12665511-Fig-2
  *** xEif4ebp1[phosAb] phos(T36/T45)[phosAb] is detectable
    *** cells: HEK293<xEif4ebp1> in BMS "treated with Ins for 5 hr"
    *** inhibited by: xEif4ebp1(T36A) [substitution]
    *** inhibited by: xEif4ebp1(T45A) [substitution]
    *** unaffected by: xEif4ebp1(S65A) [substitution]
    *** unaffected by: xEif4ebp1(T70A) [substitution]
    *** unaffected by: xEif4ebp1(S83A) [substitution]
    *** inhibited by: xEif4ebp1(T36A/T45A) [substitution]
    *** source: 10942774-Fig-2a
  *** rEif4ebp1[phosAb] phos(T36/T45)[phosAb] is increased by rErk2{act}
    *** cells: none
    *** partially inhibited by: rEif4ebp1(T36A) [substitution]
    *** partially inhibited by: rEif4ebp1(T45A) [substitution]
    *** partially inhibited by: rEif4ebp1(S65A) [substitution]
    *** partially inhibited by: rEif4ebp1(T70A) [substitution]
    *** inhibited by: rEif4ebp1(T36A/T45A) [substitution]
    *** source: 10942774-Fig-1
  *** rEif4ebp1[phosAb] phos(T36/T45)[phosAb] is increased by xMtor[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xMtor> in BMS
    *** unaffected by: Rapamycin [addition]
    *** unaffected by: rFkbp12 [addition]
    *** unaffected by: FK506 [addition]
    *** inhibited by: rFkbp12 + Rapamycin [addition]
    *** unaffected by: rFkbp12 + FK506 [addition]
    *** source: 10942774-Fig-6a

phos(S65)

  *** xEif4ebp1[phosAb] phos(S65)[phosAb] is detectable
    *** cells: HEK293<xEif4ebp1> in BMS "treated with Ins for 5 hr"
    *** inhibited by: xEif4ebp1(T36A) [substitution]
    *** inhibited by: xEif4ebp1(T45A) [substitution]
    *** inhibited by: xEif4ebp1(S65A) [substitution]
    *** inhibited by: xEif4ebp1(T70A) [substitution]
    *** unaffected by: xEif4ebp1(S83A) [substitution]
    *** source: 10942774-Fig-2c
  *** xEif4ebp1[phosAb] phos(S65)[phosAb] is detectable
    *** cells: HEK293<xEif4ebp1> in BMS "treated with Ins for 5 hr"
    *** unaffected by: xEif4ebp1(L68A/M59A) [substitution]
    *** inhibited by: xEif4ebp1(S65A) [substitution]
    *** inhibited by: xEif4ebp1(S65A/L68A/M59A) [substitution]
    *** source: 10942774-Fig-2c
  *** rEif4ebp1[phosAb] phos(S65)[phosAb] is unchanged by xMtor[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xMtor> in BMS
    *** unaffected by: Rapamycin [addition]
    *** unaffected by: rFkbp12 [addition]
    *** unaffected by: FK506 [addition]
    *** unaffected by: rFkbp12 + Rapamycin [addition]
    *** unaffected by: rFkbp12 + FK506 [addition]
    *** source: 10942774-Fig-6b

phos(T70)

  *** rEif4ebp1[phosAb] phos(T70)[phosAb] is increased by (xMtor[tAb]IP + anti-Mtor)
    *** cells: none
    *** comment: xMtor[tAb]IP from HEK293T in BMS
    *** reqs: both [omissions]
    *** unaffected by: Rapamycin [addition]
    *** unaffected by: rFkbp12 [addition]
    *** inhibited by: Rapamycin + rFkbp12 [addition]
    *** inhibited by: LY294002 [addition]
    *** source: 12665511-Fig-4
  *** rEif4ebp1[phosAb] phos(T70)[phosAb] is increased by xMtor[tAb]IP
    *** cells: none
    *** comment: xMtor[tAb]IP from HEK293T in BMS
    *** comment: xMtor[tAb]IP was "activated" with anti-Mtor after IP
    *** inhibited by: rEif4ebp1(F113A) [substitution]
    *** unaffected by: rEif4ebp1(I15A/P16A) [substitution]
    *** source: 12665511-Fig-2
  *** rEif4ebp1(T36A/T45A/S65A/S83A)[32P] phos[32P-ATP] is increased by xMtor[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xMtor> in BMS
    *** comment: increased by addition of Mtor "activating" antibody mTab1
    *** comment: unaffected by addition of Mtor "nonactivating" antibody mTab2
    *** inhibited by: Rapamycin [addition]
    *** unaffected by: FK506 [addition]
    *** source: 10942774-Fig-7
  *** rEif4ebp1(T36A/T45A/S65A/S83A)[32P] phos[32P-ATP] is increased by xMtor[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xMtor> in BMS
    *** inhibited by: xMtor(D2338A)"KD" [substitution]
    *** unaffected by: xMtor(S2035I)"CA" [substitution]
    *** comment: xMtor(S2035I)"CA" could not be inhibited with Rapamycin
    *** source: 10942774-Fig-7
  *** xEif4ebp1[phosAb] phos(T70)[phosAb] is detectable
    *** cells: HEK293<xEif4ebp1> in BMS "treated with Ins for 5 hr"
    *** partially inhibited by: xEif4ebp1(T36A) [substitution]
    *** partially inhibited by: xEif4ebp1(T45A) [substitution]
    *** unaffected by: xEif4ebp1(S65A) [substitution]
    *** inhibited by: xEif4ebp1(T70A) [substitution]
    *** unaffected by: xEif4ebp1(S83A) [substitution]
    *** partially inhibited by: xEif4ebp1(T36A/T45A) [substitution]
    *** source: 10942774-Fig-2a
  *** rEif4ebp1[phosAb] phos(T70)[phosAb] is unchanged by xMtor[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xMtor> in BMS
    *** unaffected by: Rapamycin [addition]
    *** unaffected by: rFkbp12 [addition]
    *** unaffected by: FK506 [addition]
    *** unaffected by: rFkbp12 + Rapamycin [addition]
    *** unaffected by: rFkbp12 + FK506 [addition]
    *** source: 10942774-Fig-6c






*** Rheb--

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