======================
Direct Phosphorylation
======================

  *** rEif4e phos[32P-ATP] is increased by rMnk1{act}
    *** cells: none
    *** comment: phos site was shown to be S209 by 2D-PPMapping
    *** source: 9155017-Fig-8
  *** xEif4e[tAb]IP phos[32P-ATP] is increased by rMnk1{act}
    *** cells: none
    *** IPfrom: HEK293T<xEif4e> in BMS 
    *** unaffected by: rEif4e(W73A) [substitution]
    *** inhibited by: xEif4e(S209A/T210A) [substitution]
    *** source: 9878069-Fig-9a
  *** rEif4e[32P] phos[32P-ATP] is increased by rMnk2{act}
    *** cells: none
    *** comment: rMnk2 was activated by phos with rP38a{act}
    *** partially inhibited by: rMnk2b [substitution]
    *** source: 12897141-Fig-3a

===========================
Semi-direct Phosphorylation
===========================

  *** rEif4e[32P] phos[32P-ATP] is increased by xMnk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xMnk2> in BMS
    *** source: 12897141-Fig-2d

=====================
Total Phosphorylation
=====================

  *** Eif4e[Ab]IP phos[32Pi] is detectable
    *** cells: mEFs in BMLS
    *** does not req: Mnk1 [KO]
    *** reqs: Mnk2 [KO]
    *** reqs: Mnk1 and/or Mnk2 [DKO]
    *** source: 15254222-Fig-3c
  *** Eif4e[m7GTP]IP phos[32Pi] is detectable
    *** cells: mEFs in BMLS
    *** does not req: Mnk1 [KO]
    *** reqs: Mnk2 [KO]
    *** reqs: Mnk1 and/or Mnk2 [DKO]
    *** source: 15254222-Fig-3c

=======================
Phosphorylation on S209
=======================

in serum starved cells

  *** Eif4e[phosAb] phos(S209)[phosAb] is detectable
    *** cells: mEFs in BMLS
    *** does not req: Mnk1 [KO]
    *** partially reqs: Mnk2 [KO]
    *** reqs: Mnk1 and/or Mnk2 [DKO]
    *** source: 15254222-Fig-3b
  *** Eif4e[phosAb] phos(S209)[phosAb] is detectable
    *** cells: mEFs in BMLS
    *** reqs: Mnk1 and/or Mnk2 [DKO]
    *** source: 15254222-Fig-3a

in serum replete cells

  *** Eif4e[phosAb] phos(S209)[phosAb] is detectable
    *** cells: mEFs in BMS
    *** partially reqs: Mnk1 [KO]
    *** partially reqs: Mnk2 [KO]
    *** reqs: Mnk1 and/or Mnk2 [DKO]
    *** source: 15254222-Fig-3b
  *** Eif4e[phosAb] phos(S209)[phosAb] is detectable
    *** cells: mEFs in BMS
    *** reqs: Mnk1 and/or Mnk2 [DKO]
    *** source: 15254222-Fig-3a

==================================================
Phosphorylation in response to Mnk1 overexpression
==================================================

  *** xEif4e[phosAb] phos(S209)[phosAb] is increased itpo xMnk1
    *** cells: HEK293 in BMLS
    *** inhibited by: xMnk1(T197A/T202A) [substitution]
    *** enhanced by: xMnk1(T332D) [substitution]
    *** source: 11463832-Fig-1a
  *** xEif4e[phosAb] phos(S209)[phosAb] is increased itpo xMnk1
    *** cells: HEK293 in BMLS
    *** inhibited by: xMnk1(T209A/T214A) [substitution]
    *** inhibited by: xMnk1(K78M/D191A) [substitution]
    *** enhanced by: xMnk1(T334D) [substitution]
    *** source: 11463832-Fig-1a
  *** xEif4e[phosAb] phos(S209)[phosAb] is increased itpo xMnk1
    *** cells: HEK293 in BMLS
    *** source: 11463832-Fig-2d
  *** Eif4e[phosAb] phos(S209)[phosAb] is increased itpo xMnk1
    *** cells: MCF7 in BMS
    *** inhibited by: xMnk1(T250A) [substitution]
    *** inhibited by: xMnk1(T257A) [substitution]
    *** source: 17130135-Fig-2a
  *** Eif4e[phosAb] phos(S209)[phosAb] is increased itpo xMnk1
    *** cells: MCF7 in BMS
    *** inhibited by: xMnk1(T250A) [substitution]
    *** inhibited by: xMnk1(T257A) [substitution]
    *** source: 17130135-Fig-2b
  *** Eif4e[phosAb] phos(S209)[phosAb] is increased itpo xMnk1
    *** cells: MCF7 in BMS
    *** unaffected by: Serum (10 min) [stim]
    *** unaffected by: Arsenite (30 min) [stim]
    *** source: 17130135-Fig-2b
  *** Eif4e[m7GTP]IP phos[WBMS] is increased itpo xMnk2
    *** cells: HEK293 in BMLS
    *** partially inhibited by: xMnk2(R447A/R448A) [substitution]
    *** partially inhibited by: xMnk2(L444A) [substitution]
    *** partially inhibited by: xMnk2(1-455) [substitution]
    *** inhibited by: xMnk2(1-446) [substitution]
    *** source: 12897141-Fig-4f
  *** Eif4e[m7GTP]IP phos[WBMS] is increased itpo xMnk2
    *** cells: HEK293 in BMS
    *** inhibited by: xMnk2(del(60-70))"delBR" [substitution]
    *** source: 12897141-Fig-2g
  *** Eif4e[m7GTP]IP phos[WBMS] is increased itpo xMnk2
    *** cells: HEK293 in BMS
    *** source: 12897141-Fig-2e

