======================
Direct Phosphorylation
======================

  *** rCtnnb1(1-423)[32P] phos[32P-ATP] is increased by rGsk3b
    *** cells: none
    *** enhanced by: rAxin2 [addition]
    *** source: 9566905-Fig-8b
  *** rCtnnb1[32P] phos[32P-ATP] is increased by (rGsk3b + rAxin1(298-506))
    *** cells: none
    *** reqs: rAxin1(298-506) [omission]
    *** source: 9482734-Fig-9a
  *** rCtnnb1[32P] phos[32P-ATP] is increased by rGsk3b
    *** cells: none
    *** enhanced by: rAxin2(265-483) [addition]
    *** unaffected by: rAxin2(265-412) [substitution]
    *** unaffected by: rAxin2(412-483) [substitution]
    *** source: 9566905-Fig-8a
  *** rCtnnb1[32P] phos[32P-ATP] is increased by rGsk3b
    *** cells: none
    *** inhibited by: rCtnnb1(mnr)"Cterm" [substitution]
    *** source: 10074433-Fig-3a

on S33/S37/S41/S45

  *** rCtnnb1[phosAb] phos(S33/S37)[phosAb] is increased by rGsk3b
    *** cells: none
    *** source: 11955436-Fig-1e
  *** rCtnnb1[phosAb] phos(S33/S37/T41)[phosAb] is increased by rGsk3b
    *** cells: none
    *** source: 11955436-Fig-1e
  *** rCtnnb1[phosAb] phos(T41)[phosAb] is increased by rGsk3b
    *** cells: none
    *** source: 11955436-Fig-1e
  *** rCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased by rGsk3b
    *** cells: none
    *** source: 11955436-Fig-1e

===========================
SemiDirect Phosphorylation
===========================

on S33

  *** xCtnnb1[tAb]IP phos(S33)[phosAb] is increased by rGsk3b
    *** cells: none
    *** IPfrom: HEK293T<xCtnnb1> in BMS
    *** enhanced by: rCsnk1d [addition]
    *** source: 12000790-Fig-5a

on T41/S45

  *** xCtnnb1[tAb]IP phos(T41/S45)[phosAb] is increased by rGsk3b
    *** cells: none
    *** IPfrom: HEK293T<xCtnnb1> in BMS
    *** enhanced by: rCsnk1d [addition]
    *** source: 12000790-Fig-5a

Gsk3b does not phos Ctnnb1 on S45

  *** xCtnnb1[tAb]IP phos(S45)[phosAb] is detectable-but unchanged by rGsk3b
    *** cells: none
    *** IPfrom: HEK293T<xCtnnb1> in BMS
    *** source: 12051714-Fig-2

rAxin cannot phos Ctnnb1 on S33 by itself

  *** xCtnnb1[tAb]IP phos(S33)[phosAb] is unchanged by xAxin1[tAb]IP
    *** cells: none
    *** IP1from: HEK293T<xCtnnb1> in BMS
    *** IP1from: HEK293T<xAxin1> in BMS
    *** source: 12000790-Fig-5a

rAxin1 can phos Ctnnb1 on T41/S45 by itself
except that Axin1 is not a kinase - kinase must come from IP1 or IP2

  *** xCtnnb1[tAb]IP phos(T41/S45)[phosAb] is increased by xAxin1[tAb]IP
    *** cells: none
    *** IP1from: HEK293T<xCtnnb1> in BMS
    *** IP1from: HEK293T<xAxin1> in BMS
    *** source: 12000790-Fig-5a

=================================================
Phosphorylation of Ctnnb1 by Gsk3b overexpression
=================================================

on S33

  *** xCtnnb1[phosAb] phos(S33)[phosAb] is increased itpo xGsk3b
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** source: 12000790-Fig-1b

on S33/S37

  *** xCtnnb1[tAb]IP phos(S33/S37)[phosAb] is increased itpo xGsk3b
    *** cells: Xembryos<xCtnnb1> in BMS
    *** inhibited by: xGsk3b(mnr)"KM" [substitution]
    *** source: 11955436-Fig-2a

on S33/S37/T41

  *** xCtnnb1[tAb]IP phos(S33/S37/T41)[phosAb] is increased itpo xGsk3b
    *** cells: HEK293T<xCtnnb1> in BMS
    *** bkg inhibited by: xCtnnb1(S45A) [substitution]
    *** source: 11967263-Fig-7
  *** xCtnnb1[tAb]IP phos(S33/S37/T41)[phosAb] is increased itpo xGsk3b
    *** cells: HEK293T<xCtnnb1> in BMS
    *** unaffected by: xGsk3b(S9A) [substitution]     
    *** inhibited by: xGsk3b(Y216F) [substitution]
    *** partially inhibited by: xGsk3b(L128A) [substitution]
    *** source: 11967263-Fig-2a

  -------------- contradictions ------------------------------

  *** xCtnnb1[phosAb] phos(S37)[phosAb] is unchanged itpo xGsk3b
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** source: 12000790-Fig-1b
  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is unchanged itpo xGsk3b
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** source: 12000790-Fig-1b

on T41

  *** xCtnnb1[tAb]IP phos(T41)[phosAb] is increased itpo xGsk3b
    *** cells: Xembryos<xCtnnb1> in BMS
    *** inhibited by: xGsk3b(mnr)"KM" [substitution]
    *** source: 11955436-Fig-2a

not on S45

  *** xCtnnb1[tAb]IP phos(S45)[phosAb] is detectable-but unchanged itpo xGsk3b
    *** cells: Xembryos<xCtnnb1> in BMS
    *** unaffected by: xGsk3b(mnr)"KM" [substitution]
    *** source: 11955436-Fig-2a
  *** xCtnnb1(T41A)[tAb]IP phos(S45)[phosAb] is detectable-but unchanged itpo xGsk3b
    *** cells: HEK293T<xCtnnb1(T41A)> in BMS
    *** source: 12051714-Fig-3a

=================================================
Phosphorylation of Ctnnb1 by Axin1 overexpression
=================================================

on S33

  *** xCtnnb1[phosAb] phos(S33)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** enhanced by: xGsk3b [addition]
    *** source: 12000790-Fig-1b
  *** xCtnnb1[phosAb] phos(S33)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** enhanced by: xGsk3b [addition]
    *** source: 12000790-Fig-3c
  *** xCtnnb1[phosAb] phos(S33)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** enhanced by: xGsk3b [addition]
    *** source: 12000790-Fig-6b
  *** xCtnnb1[phosAb] phos(S33)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** inhibited by: LiCl [addition]
    *** source: 12000790-Fig-3a
  *** xCtnnb1[phosAb] phos(S33)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** inhibited by: xAxin1(L525P)"Gsk3b-binding-mut" [substitution]
    *** source: 12000790-Fig-3c

on S37

  *** xCtnnb1[phosAb] phos(S37)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** enhanced by: xGsk3b [addition]
    *** source: 12000790-Fig-1b

on T41/S45

  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS
    *** partially inhibited by: CKI7 [addition]
    *** source: 12000790-Fig-5c
  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS
    *** unaffected by: xCsnk1e [addition]
    *** partially inhibited by: xCsnk1e(D128N)"DN" [addition]
    *** partially inhibited by: xCsnk1e(K58R)"KD" [addition]
    *** source: 12000790-Fig-5b
  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** enhanced by: xGsk3b [addition]
    *** source: 12000790-Fig-1b
  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** enhanced by: xGsk3b [addition]
    *** source: 12000790-Fig-2a
  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** enhanced by: xGsk3b [addition]
    *** source: 12000790-Fig-6b
  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** unaffected by: LiCl [addition]
    *** source: 12000790-Fig-3a
  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** unaffected by: xAxin1(L525P)"Gsk3b-binding-mut" [substitution]
    *** source: 12000790-Fig-3c
  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** unaffected by: xCtnnb1(S29F) [substitution]
    *** partially inhibited by: xCtnnb1(S33Y) [substitution]
    *** unaffected by: xCtnnb1(S37A) [substitution]
    *** unaffected by: xCtnnb1(T41A) [substitution]
    *** inhibited by: xCtnnb1(S45F) [substitution]
    *** source: 12000790-Fig-2a
  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** unaffected by: xGsk3b [addition]
    *** source: 12000790-Fig-3c

on S33/S37/T41

  *** xCtnnb1[tAb]IP phos(S33/S37/T41)[phosAb] is increased itpo xAxin1
    *** cells: Xembryos<xCtnnb1> in BMS
    *** unaffected by: xAxin1(mnr)"delRGS" [substitution]
    *** source: 11955436-Fig-3b

====================================================================
Phosphorylation of Ctnnb1 by overexpression of Axin1 and Gsk3b
====================================================================

on S33

  *** xCtnnb1[phosAb] phos(S33)[phosAb] is increased itpo (xAxin1 + xGsk3b)
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** unaffected by: xCtnnb1(S29F) [substitution]
    *** inhibited by: xCtnnb1(S33Y) [substitution]
    *** inhibited by: xCtnnb1(S37A) [substitution]
    *** inhibited by: xCtnnb1(T41A) [substitution]
    *** inhibited by: xCtnnb1(S45F) [substitution]
    *** source: 12000790-Fig-2a

on S37

  *** xCtnnb1[phosAb] phos(S37)[phosAb] is increased itpo (xAxin1 + xGsk3b)
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** partially inhibited by: xCtnnb1(S29F) [substitution]
    *** partially inhibited by: xCtnnb1(S33Y) [substitution]
    *** inhibited by: xCtnnb1(S37A) [substitution]
    *** inhibited by: xCtnnb1(T41A) [substitution]
    *** inhibited by: xCtnnb1(S45F) [substitution]
    *** source: 12000790-Fig-2a

on S41 or S45

  *** xCtnnb1[phosAb] phos(T41/S45)[phosAb] is increased itpo (xAxin1 + xGsk3b)
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** unaffected by: xCtnnb1(S29F) [substitution]
    *** unaffected by: xCtnnb1(S33Y) [substitution]
    *** unaffected by: xCtnnb1(S37A) [substitution]
    *** partially inhibited by: xCtnnb1(T41A) [substitution]
    *** inhibited by: xCtnnb1(S45F) [substitution]
    *** source: 12000790-Fig-2a




==================================================================================
Detectable Phosphorylation of xCtnnb1 on S33/S37/T41 requires phosphorylatable T41
==================================================================================

  *** xCtnnb1[tAb]IP phos(S33/S37/T41)[phosAb] is detectable
    *** cells: HEK293T<xCtnnb1> in BMS
    *** inhibited by: xCtnnb1(S45A) [substitution]
    *** source: 11967263-Fig-7


=================================================
Inhibition of Phosphorylation by Gsk3 inhibitors
=================================================

Ctnnb1 phos(S33) requires Gsk3s

  *** Ctnnb1[phosAb] phos(S33)[phosAb] is detectable
    *** cells: HELA in BMS "treated with MG132"
    *** inhibited by: LiCl [addition]
    *** source: 12000790-Fig-5d

Ctnnb1 phos(S45) does not require Gsk3s

  *** Ctnnb1[phosAb] phos(T41/S45)[phosAb] is detectable
    *** cells: HELA in BMS "treated with MG132"
    *** unaffected by: LiCl [addition]
    *** source: 12000790-Fig-5d

Is there enough evidence here to conclude that Ctnnb1 needs to be "primed" by
phos on S45 before Gsk3s can phos the remaining string?

=====================================================
Ctnnb1 phosphorylation by Gsk3b is inhibited by Dvl1
=====================================================

  *** rCtnnb1[32P] phos[32P-ATP] is increased by (rAxin1 + rGsk3b)
    *** cells: none
    *** inhibited by: rDvl1 [addition]
    *** partially inhibited by: rDvl1(140-670) [addition]
    *** unaffected by: rDvl1(del(282-336))"delPDZ" [addition]
    *** source: 10330181-Fig-6b
  *** rCtnnb1[32P] phos[32P-ATP] is increased by (rAxin1 + rGsk3b)
    *** cells: none
    *** partially inhibited by: rDvl1 [addition]
    *** source: 10330181-Fig-6a


