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Coprecipitations
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Axin1 required features

  *** xAxin1[tAb] copptby[WB] xBtrc[tAb] is detectable
    *** cells: HEK293T<xBtrc><xAxin1> in BMS
    *** inhibited by: xBtrc(1-140)"delF/WD" [substitution]
    *** inhibited by: xBtrc(1-214)"delWD" [substitution]
    *** inhibited by: xBtrc(194-569)"delN/F" [substitution]
    *** unaffected by: xBtrc(del(140-194))"delFbox" [substitution]
    *** source: 10228155-Fig-2b

Requires some form of Ctnnb1 phosphorylation

  *** xAxin1[tAb] copptby[WB] xBtrc[tAb] is detectable
    *** cells: HEK293T<xAxin1><xBtrc><xCtnnb1> in BMS
    *** inhibited by: xCtnnb1(S29A/S33A/S37A/T41A/S45A) [substitution]
    *** source: 10228155-Fig-2c

No extras

  *** xAxin1[tAb] copptby[WB] xBtrc[tAb] is detectable
    *** cells: HEK293T<xBtrc><xAxin1> in BMS
    *** source: 10228155-Fig-1a
  *** xAxin1[tAb] copptby[WB] xBtrc[tAb] is detectable
    *** cells: HEK293T<xBtrc><xAxin1> in BMS
    *** source: 10228155-Fig-3b
  *** xBtrc[tAb] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293T<xBtrc><xAxin1> in BMS
    *** source: 10228155-Fig-1b

======================================================
Coprecipitation with other members of the Axin complex
======================================================

Ctnnb1--  direct interaction requires phos on (S33A/S37A/T41A/S45A)

  *** rBtrc[35S] boundby[WB] rCtnnb1{phos}[GST] is detectable
    *** cells: none
    *** comment: rCtnnb1 was phosed by rGsk3b
    *** unaffected by: rCtnnb1(S23Y) [substitution]
    *** inhibited by: rCtnnb1(D32N) [substitution]
    *** inhibited by: rCtnnb1(S33Y) [substitution]
    *** inhibited by: rCtnnb1(G34V) [substitution]
    *** inhibited by: rCtnnb1(S37F) [substitution]
    *** partially inhibited by: rCtnnb1(T41A) [substitution]
    *** unaffected by: rCtnnb1(S45F) [substitution]
    *** inhibited by: rCtnnb1(S33A/S37A/T41A/S45A) [substitution]
    *** source: 11955436-Fig-1b
  *** rBtrc[35S] boundby[WB] rCtnnb1{phos}[GST] is detectable
    *** cells: none
    *** comment: requires pre-phosphorylation of Ctnnb1 by rGsk3b
    *** source: 11955436-Fig-1b
  *** xBtrc[tAb] copptby[WB] xCtnnb1[tAb] is detectable
    *** cells: Xembryos<xBtrc><xCtnnb1> in BMS
    *** inhibited by: xCtnnb1(S33Y) [substitution]
    *** inhibited by: xCtnnb1(S37F) [substitution]
    *** inhibited by: xCtnnb1(T41A) [substitution]
    *** inhibited by: xCtnnb1(S45F) [substitution]
    *** inhibited by: xCtnnb1(S33A/S37A/T41A/S45A) [substitution]
    *** source: 11955436-Fig-1c

