===============================================
Nckipsd regions required for binding to ArhGap5
===============================================

  *** rArhGap5[35S] boundby[WB] rNckipsd(159-348)[GST] is detectable
    *** cells: none
    *** source: 14765113-Fig-2c
  *** rArhGap5[35S] boundby[WB] rNckipsd(mnr)"Cterm"[GST] is detectable
    *** cells: none
    *** source: 14765113-Fig-2c
  *** rArhGap5[35S] boundby[WB] rNckipsd(mnr)"SH3"[GST] is detectable
    *** cells: none
    *** source: 14765113-Fig-2c


===============================================
ArhGap5 regions required for binding to Nckipsd
===============================================



=======================================================
Constitutive association in treated and untreated cells
=======================================================

unaffected by Egf treatment

  *** ArhGap5[Ab] copptby[WB] Nckipsd[Ab] is detectable-but unchanged irt Egf (10 min)
    *** cells: COS7 in BMS
    *** source: 14765113-Fig-1b
  *** ArhGap5[Ab] copptby[WB] xNckipsd[tAb] is detectable-but unchanged irt Egf (10 min)
    *** cells: COS7<xNckipsd><xVav2> in BMS "membrane-fractions"
    *** unaffected by: xNckipsd(del(170-249))"dPro" [substitution]
    *** unaffected by: xNckipsd(Y161F) [substitution]
    *** unaffected by: xNckipsd(Y227F) [substitution]
    *** unaffected by: xNckipsd(Y161F/Y227F) [substitution]
    *** comment: Yphos of coppts is inhibited by xNckipsd(Y161F) [substitution]
    *** comment: Yphos of coppts is inhibited by xNckipsd(del(170-249))"dPro" [substitution]
    *** source: 14765113-Fig-2d
  *** ArhGap5[Ab] copptby[WB] xNckipsd[tAb] is detectable-but unchanged irt Egf (times)
    *** cells: COS7<xNckipsd> in BMS
    *** times: 0+ 5+ 10+ 30+ min
    *** source: 14765113-Fig-1e
  *** xNckipsd[tAb] copptby[WB] xArhGap5[tAb] is detectable-but unchanged irt Egf (10 min)
    *** cells: COS7<xNckipsd><xArhGap5> in BMS "Membrane-fraction"
    *** source: 14765113-Fig-1b
  *** xNckipsd[tAb] copptby[WB] xVav2[tAb] is detectable-but unchanged irt Egf (10 min)
    *** cells: COS7<xNckipsd><xVav2> in BMS "Membrane-fraction"
    *** source: 14765113-Fig-1b

=======================================================
Locations are Unsure - put it all in the CLc by default
=======================================================

  *** ArhGap5[Ab] infraction(membrane)[WB] is detectable
    *** cells: HELA in BMS
    *** reqs: Nckipsd [RNAi]
    *** source: 14765113-Fig-6b
  *** ArhGap5[Ab] prot-exp[WB] is detectable
    *** cells: HELA in BMS
    *** does not req: Nckipsd [RNAi]
    *** source: 14765113-Fig-6b
  *** ArhGap5[Ab]IP prot-exp[WB] is detectable
    *** cells: HELA in BMS "membrane-fractions"
    *** reqs: Nckipsd [RNAi]
    *** source: 14765113-Fig-6a
