============================================================
direct phosphorylation of Akt1 on T308 but not S473 by Pdpk1
============================================================

  *** rAkt1[32P] phos(T308)[HPLC-PAA-ED] is increased by pPdpk1
    *** cells: none
    *** source: 9094314-Fig-1a
  *** xAkt1(1-467)[tAb]IP phos(T308)[phosAb] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xAkt1(1-467)> in BMS
    *** unaffected by: rAkt1(1-467/R144A) [substitution]
    *** unaffected by: rAkt1(1-467/R200A) [substitution]
    *** source: 12374740-Fig-6d
  --------------------------------------------------------------
  *** rAkt1[32P] phos(S473)[HPLC-PAA-ED] is unchanged by pPdpk1
    *** cells: none
    *** source: 9094314-Fig-1a
  --------------------------------------------------------------
  *** rAkt1[32P] phos[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** comment: phos reqs PIP3 in kinase buffer
    *** source: 9427642-Fig-4a
  *** rAkt1[32P] phos[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** comment: phos reqs PIP3 in kinase buffer
    *** source: 9768361-Fig-4
  *** rAkt1[32P] phos[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** source: 9094314-Fig-1a

===========================
Semi-direct phosphorylation
===========================

  *** rAkt1[phosAb] phos(S473)[phosAb] is unchanged by Pdpk1[Ab]IP
    *** cells: none
    *** IPfrom: HELA in BMS
    *** source: 21464307-Fig-3b
  *** rAkt1[phosAb] phos(T308)[phosAb] is increased by Pdpk1[Ab]IP
    *** cells: none
    *** IPfrom: HELA in BMS
    *** source: 21464307-Fig-3b

requires an intact Pdpk1 "PIF-binding Pocket" (around L155)

  *** xAkt1(118-480)[GST]IP phos(T308)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(118-480)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2d
  *** xAkt1(118-480)[GST]IP phos(T308)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** comment: kinase reaction contained PIP3
    *** IPfrom: HEK293<xAkt1(118-480)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** partially inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3d
  *** xAkt1(118-480)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(118-480)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2d
  *** xAkt1(118-480)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(118-480)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** partially inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3d
  *** xAkt1(118-480/S473D)[GST]IP phos(T308)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** comment: kinase reaction contained PIP3
    *** IPfrom: HEK293<xAkt1(118-480/S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2d
  *** xAkt1(118-480/S473D)[GST]IP phos(T308)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** comment: kinase reaction contained PIP3
    *** IPfrom: HEK293<xAkt1(118-480/S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3d
  *** xAkt1(118-480/S473D)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** comment: kinase reaction contained PIP3
    *** IPfrom: HEK293<xAkt1(118-480/S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** partially inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2d
  *** xAkt1(118-480/S473D)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** comment: kinase reaction contained PIP3
    *** IPfrom: HEK293<xAkt1(118-480/S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** partially inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3d
  *** Akt1(S473D)[GST]IP phos(T308)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2c
  *** Akt1(S473D)[GST]IP phos(T308)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: PIFtide [addition]
    *** source: 11500365-Fig-3c
  *** Akt1(S473D)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2c
  *** Akt1(S473D)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: PIFtide [addition]
    *** source: 11500365-Fig-3c
  *** xAkt1[GST]IP phos(T308)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2c
  *** xAkt1[GST]IP phos(T308)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: PIFtide [addition]
    *** source: 11500365-Fig-3c
  *** xAkt1[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2c
  *** xAkt1[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: PIFtide [addition]
    *** source: 11500365-Fig-3c


===================================
direct activation of Akt1 by Pdpk1
===================================

  *** rAkt1 IVKA(peptide)[32P-ATP] is increased by pPdpk1
    *** cells: none
    *** source: 9094314-Fig-1a
  *** xAkt1(1-467)[tAb]IP IVKA(Crosstide)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xAkt1(1-467)> in BMS
    *** enhanced by: pHMMSK [addition]
    *** unaffected by: HMMSK [addition]
    *** source: 12374740-Fig-6c
  *** xAkt1(1-467)[tAb]IP IVKA(Crosstide)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xAkt1(1-467)> in BMS
    *** unaffected by: rAkt1(1-467/R144A) [substitution]
    *** inhibited by: rAkt1(1-467/R200A) [substitution]
    *** source: 12374740-Fig-6c

=======================================
Semi-direct activation of Akt1 by Pdpk1
=======================================

requires an intact Pdpk1 "PIF-binding Pocket" (around L155)

  *** xAkt1(118-480)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(118-480)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2d
  *** xAkt1(118-480)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(118-480)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** partially inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3d
  *** xAkt1(118-480/S473D)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(118-480/S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** partially inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2d
  *** xAkt1(118-480/S473D)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** comment: kinase reaction contained PIP3
    *** IPfrom: HEK293<xAkt1(118-480/S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** partially inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3d
  *** Akt1(S473D)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2c
  *** Akt1(S473D)[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(S473D)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: PIFtide [addition]
    *** source: 11500365-Fig-3c
  *** xAkt1[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2c
  *** xAkt1[GST]IP IVKA(peptide)[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** comment: kinase reaction contained PIP3
    *** unaffected by: PIFtide [addition]
    *** source: 11500365-Fig-3c

========================================
Kinase Activity in the presence of Pdpk1
========================================
does not req Pdpk1 kinase activity but does req membrane localization

  *** xAkt1(S473D)[Ab]IP IVKA(MBP)[32P-ATP] is increased itpo xPdpk1
    *** cells: PAE<xAkt1(S473D)> in BMLS
    *** unaffected by: xPdpk1(R474A) [substitution]
    *** source: 9637919-Fig-4a
  *** xAkt1(S473D)[Ab]IP IVKA(MBP)[32P-ATP] is increased itpo xPdpk1
    *** cells: PAE<xAkt1(S473D)> in BMLS
    *** enhanced by: xPdpk1(myr) [substitution]
    *** enhanced by: xPdpk1(myr/R474A) [substitution]
    *** source: 9637919-Fig-7

=======================================================
Akt1 is phosphorylated on T308 in the presence of Pdpk1
=======================================================

  *** Akt1[phosAb] phos(T308)[phosAb] is increased itpo xPdpk1
    *** cells: HEK293 in BMS
    *** inhibited by: xPdpk1(K11N)"KD" [substitution]
    *** unaffected by: xPdpk1(mnr)"PH-domain-mut" [substitution]
    *** unaffected by: xPdpk1(del(1-50))"Nterm-mut" [substitution]
    *** source: 11889038-Fig-5b
  *** Akt1[phosAb] phos(T308)[phosAb] is detectable
    *** cells: HEK293T in BMS
    *** enhanced by: xPdpk1 [addition]
    *** inhibited by: xPdpk1(Y9F/Y373F/Y376F) [addition]
    *** source: 18616680-Fig-3a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased itpo xPdpk1
    *** cells: HEK293T in BMS
    *** bkg inhibited by: xNprl2 [addition]
    *** source: 18616680-Fig-3a


========================
Effect of Freud and PIP3
========================

  *** rAkt1[phosAb] phos(T308)[phosAb] is increased by PIP3
    *** cells: none
    *** comment: reaction mix contains rPdpk1
    *** source: 18662999-Fig-5g

  *** rAkt1[phosAb] phos(T308)[phosAb] is increased by rFreud1
    *** cells: none
    *** comment: reaction mix contains (rPdpk1 + PIP3)
    *** reqs: PIP3 [omission]
    *** source: 18662999-Fig-5g

  *** Akt1[phosAb] phos(T308)[phosAb] is detectable
    *** cells: HEK293T in BMS
    *** reqs: Freud1 [RNAi]
    *** source: 18662999-Fig-3a
  *** Akt1[Ab]IP IVKA(Gsk3?)[32P-ATP] is detectable
    *** cells: HEK293T in BMS
    *** reqs: Freud1 [RNAi]
    *** source: 18662999-Fig-3b

  *** xAkt1(S473D)[Ab]IP IVKA(MBP)[32P-ATP] is decreased irt Wortmannin (90 min)
    *** cells: PAE<xAkt1(S473D)><xPdk1> in BMLS
    *** source: 9637919-Fig-5
  *** Akt1[phosAb] phos(S473)[phosAb] is decreased irt Wortmannin (30 min)
    *** cells: HEK293 in BMLS
    *** source: 16687399-Fig-2a

















