======================================
RapGef1 to activated EgfR requires Crk
======================================

  *** xRapGef1[Ab] copptby[WB] xEgfR[Ab] is increased irt Egf (3 min)
    *** cells: HEK293T<xCrkI> in BMS
    *** reqs: xCrkI [omission]
    *** source: 9268367-Fig-8

=====================================
Tyrosine phosphorylation requires Src
=====================================

  *** RapGef1[Ab] Yphos[pYAb-ppt] is decreased irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0++ 2 5+ 30+ min
    *** source: 11466412-Fig-3c

  --- requires Src??? ---     see rule 393c
  *** RapGef1[Ab]IP Yphos[pYAb] is increased irt Egf (1 min)
    *** cells: NIH3T3<xEgfR><xAbi1> in BMLS
    *** inhibited by: PP1 [addition]
    *** source: 16182283-Fig-6
  *** RapGef1[Ab]IP Yphos[pYAb] is increased irt Egf (1 min)
    *** cells: NIH3T3<xEgfR> in BMLS
    *** inhibited by: PP1 [addition]
    *** source: 16182283-Fig-6


=========================
Coprecipitation with CrkL
=========================

  *** RapGef1[Ab] copptby[WB] CrkL[Ab] is detectable-but unchanged irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0+ 5+ 10+ min
    *** source: 11466412-Fig-4c


===================================
release from Crk,CrL at later times
===================================

  *** RapGef1[Ab] copptby[WB] Crk[Ab] is decreased irt Egf  (times)
    *** cells: PC12 in BMLS
    *** times: 0++ 2++ 5+ 15+ 30 60 min
    *** source: 11278445-Fig-4b
