============================================================
Ikba relocation to complex containing Ikk1 and Ikk2 requires
============================================================

Ikk2--  phos sites but no kinase activity

  *** Ikba[Ab] copptby[WB] Ikk1[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Ikk2 [KO]
    *** source: 14636585-Fig-2f
  *** Ikba[Ab] copptby[WB] xIkk2[tAb] is increased irt Tnf (5 min)
    *** cells: mEFs<Ikk2~null> in BMS "on gelatin"
    *** unaffected by: xIkk2(K44M)"KD" [substitution]
    *** source: 14636585-Fig-4b
  *** Ikba[Ab] copptby[WB] xIkk2[tAb] is increased irt Tnf (5 min)
    *** cells: mEFs<Ikk2~null> in BMS "on gelatin"
    *** inhibited by: xIkk2(K44M/S177A/S181A) [substitution]
    *** source: 14636585-Fig-4c

Mekk3-- activated

  *** Ikba[Ab] copptby[WB] Ikk1[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Mekk3 [KO]
    *** source: 14636585-Fig-2f
  *** Ikba[Ab] copptby[WB] Ikk2[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Mekk3 [KO]
    *** source: 14636585-Fig-2d
  *** Ikba[Ab] copptby[WB] Ikk2[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Mekk3 [KO]
    *** source: 14636585-Fig-2f
  *** Ikba[Ab] copptby[WB] Ikk2[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Mekk3 [KO]
    *** source: 14636585-Fig-2g
  *** Ikba[Ab] copptby[WB] xMekk3[tAb] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** source: 14636585(D)

Nemo-- need evidence that Nemo is ubiq

  *** Ikba[Ab] copptby[WB] Ikk2[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Nemo [KO]
    *** source: 14636585-Fig-2d

no extras--

  *** Ikba[Ab] copptby[WB] Ikk2[Ab] is increased irt Tnf (times)
    *** cells: mEFs in BMS "on gelatin"
    *** times: 0 5+ 60 min
    *** source: 14636585-Fig-3a
  *** Ikba[Ab] copptby[WB] Ikk2[Ab] is increased irt Tnf (times)
    *** cells: mEFs in BMS "on gelatin"
    *** times: 0 2++ 5+ 15 min
    *** source: 14636585-Fig-3c

=========================
Rela goes where Ikba goes
=========================

  *** Ikba[Ab] copptby[WB] Rela[Ab] is detectable
    *** cells: mEFs in BMS "on gelatin"
    *** unaffected by: Tnf (5 min) [stim]
    *** source: 14636585-Fig-2b
  *** Rela[Ab] copptby[WB] Ikba[Ab] is detectable
    *** cells: mEFs in BMS "on gelatin"
    *** unaffected by: Tnf (5 min) [stim]
    *** source: 14636585-Fig-2c

============================================================
Rela relocation to complex containing Ikk1 and Ikk2 requires
============================================================
see 14636585 for evidence that Ikbb/Mekk2 can substitute for Ikba/Mekk3

not Ikba--  can be substituted with Ikbb

  *** Rela[Ab] copptby[WB] Ikk1[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Ikk2 [KO]
    *** does not req: Ikba [KO]
    *** source: 14636585-Fig-2f
  *** Ikk2[Ab] copptby[WB] Rela[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** does not req: Ikba [KO]
    *** source: 14636585-Fig-2b

Ikk2-- phos sites but not kinase activity

  *** Rela[Ab] copptby[WB] xIkk2[tAb] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** unaffected by: xIkk2(K44M)"KD" [substitution]
    *** inhibited by: xIkk2(K44M/S177A/S181A) [substitution]
    *** source: 14636585(D)

not Mekk3--

  *** Rela[Ab] copptby[WB] Ikk1[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Ikk2 [KO]
    *** does not req: Mekk3 [KO]
    *** source: 14636585-Fig-2f
  *** Ikk2[Ab] copptby[WB] Rela[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** does not req: Mekk3 [KO]
    *** source: 14636585-Fig-2b
  *** Rela[Ab] copptby[WB] Ikk2[Ab] is increased irt Tnf (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** does not req: Mekk3 [KO]
    *** source: 14636585-Fig-2g
  *** Rela[Ab] copptby[WB] Ikk2[Ab] is increased irt Tnf (times)
    *** cells: mEFs in BMS "on gelatin"
    *** times: 0 5+ 60+ min
    *** does not req: Mekk3 [KO]
    *** source: 14636585-Fig-3a










