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Myc can be ubiquitinated directly by rFbxw7, rUbiq, rUbe1, and rUbe2d1
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  *** rMyc[Ab] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d1 + rFbxw7)
    *** cells: none
    *** source: 15103331-Fig-2a
  *** rMyc[35S] upshift[WBMS] is detectable
    *** cells: none
    *** comment: reaction mix contains rFbxw7, rUbiq, rUbe1, rUbe2d1, and rGsk3b
    *** inhibited by: rMyc(T58A) [substitution]
    *** source: 15103331-Fig-5c
  *** rMyc[35S] upshift[WBMS] is increased by rGsk3b
    *** cells: none
    *** comment: reaction mix also contains rUbiq, rUbe1, rUbe2d1, rFbxw7
    *** unaffected by: rErk2 [addition]
    *** source: 15103331-Fig-5b

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Myc ubiq requires phosphorylation on T58
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  *** rMyc[35S] upshift[WBMS] is increased by rFbxw7
    *** cells: none
    *** comment: reaction mix also contains rUbiq, rUbe1, rUbe2d1, rErk2, and rGsk3b
    *** inhibited by: rMyc(T58A) [substitution]
    *** source: 15103331-Fig-5a
  *** rMyc[Ab] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d1 + rFbxw7)
    *** cells: none
    *** inhibited by: rMyc(T58A/S62A) [substitution]
    *** source: 15103331-Fig-3c

  *** xMyc[Ab] ubiq[UbAb-ppt] is detectable
    *** cells: REF52 in BMLS "treated with Lactacystin for 6 hr"
    *** comment: precipitated xMyc is phosphorylated on T58 [phosAb]
    *** comment: precipitated xMyc is not phosphorylated on S62 [phosAb]
    *** source: 15048125-Fig-1a

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Fbxw7 binds to Myc phosphorylated on T58
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  *** rFbxw7g[tAb] boundby[WB] rMyc(51-69){phos(T58/T62)}[agarose] is detectable
    *** cells: none
    *** inhibited by: rMyc(51-69) [substitution]
    *** unaffected by: rMyc(51-69){phos(T58)} [substitution]
    *** inhibited by: rMyc(51-69){phos(T62)} [substitution]
    *** source: 15150404-Fig-3d
  *** Myc[Ab] copptby[WB] xFbxw7[tAb] is detectable
    *** cells: HELA in BMS
    *** source: 15150404-Fig-2b
  *** Myc[Ab] copptby[WB] xFbxw7g(mnr)"WD"[tAb] is detectable 
    *** cells: HEK293<xFbxw7g(mnr)"WD"> in BMS
    *** source: 15150404-Fig-3c
  *** xMyc[Ab] copptby[WB] xFbxw7g(mnr)"WD"[tAb] is detectable 
    *** cells: HEK293<xMyc><xFbxw7g(mnr)"WD"> in BMS
    *** source: 15150404-Fig-3c
  *** Myc[Ab] copptby[WB] xFbxw7g[tAb] is detectable
    *** cells: HELA in BMS
    *** source: 15150404-Fig-2b
  *** xMyc[Ab] copptby[WB] xFbxw7g[tAb] is detectable 
    *** cells: HEK293<xMyc><xMax><xFbxw7g> in BMS
    *** source: 15150404-Fig-2a












