No evidence found that Tak1 is recruited to IL1R1 irt IL1
All based on coppt with other IL1R1 binding proteins and similarity with
other dishes

===================
Tak1 coppt by Traf6
===================

Mekk3--

  *** Tak1[Ab] copptby[WB] Traf6[Ab] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 10+ 20+ min
    *** reqs: Mekk3 [KO]
    *** source: 19843958-Fig-3f

Tak1 attributes

  *** Traf6[Ab] copptby[WB] xTak1[His] is increased irt IL1b (times)
    *** cells: HEK293T<xTak1> in BMS
    *** times: 0+ 5+ 10++ 15+ min
    *** unaffected by: xTak1(K158R) [substitution]
    *** source: 20038579-Fig-S3b

no extras

  *** Tak1[Ab] copptby[WB] Traf6[Ab] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 10++ 30++ 60+ min
    *** source: 12496252-Fig-2b
  *** Traf6[Ab] copptby[WB] Tak1[Ab] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 1 5+++ 10++ 30++ 60+ 120 240 min
    *** source: 12242293-Fig-4a
  *** Tak1[Ab] copptby[WB] Traf6[Ab] is increased irt IL1 (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 10++ 20+ min
    *** source: 10882101(D)
  *** Traf6[Ab] copptby[WB] Tak1[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 1+ 2++ 5++ 10+ 20 min
    *** source: 11259596-Fig-9a
  *** Traf6[Ab] copptby[WB] Tak1[Ab] is increased irt IL1b (5 min)
    *** cells: HEK293 in BMS
    *** source: 11259596-Fig-9b
  *** Traf6[Ab] copptby[WB] Tak1[Ab] is unchanged irt IL1b (5 min)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** source: 11259596-Fig-9b
  *** Traf6[Ab] copptby[WB] Tak1[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3++ 15+ min
    *** source: 14633987-Fig-5a
  *** Tak1[Ab] copptby[WB] Traf6[Ab] is increased irt IL1 (10 min)
    *** cells: HEK293<xIL1R1> in BMS
    *** source: 10094049-Fig-1b
  *** xTak1[Ab] copptby[WB] Traf6[Ab] is increased irt IL1 (times)
    *** cells: mEFs<Tak1~null> in BMS
    *** times: 0 10+ 20+ min
    *** inhibited by: xTak1(K209R) [substitution]
    *** source: 19843958-Fig-3a
  *** Traf6[Ab] copptby[WB] Tak1[Ab] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 10+ 20 30 min
    *** source: 19843958-Fig-3d
  *** Tab2[Ab] copptby[WB] Traf6[Ab] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 10++ 30++ 60+ min
    *** source: 12496252-Fig-2b

===================
Tabs coppt by Traf6
===================

Tab1--

  *** Tab1[Ab] copptby[WB] Traf6[Ab] is increased irt IL1 (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 10++ 20+ min
    *** source: 10882101(D)
  *** Tab1[Ab] copptby[WB] Traf6[Ab] is increased irt IL1 (10 min)
    *** cells: HEK293<xIL1R1> in BMS
    *** source: 10094049-Fig-1b

Tab2--

  *** Traf6[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (10 min)
    *** cells: HEK293 in BMS
    *** source: 12242293-Fig-4b
  *** Traf6[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (10 min)
    *** cells: HEK293I1A<Irak1~null><xIrak1> in BMS
    *** reqs: xIrak1 [omission]
    *** source: 12242293-Fig-4b
  *** Traf6[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 1 2++ 5+++ 10++ 30++ 60+ 120 240 min
    *** source: 12242293-Fig-4a
  *** Traf6[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 2+ 5+ 10++ 20+ 30+ 40+ 50+ 60+ min
    *** source: 18180283-Fig-5a
  *** Tab2[Ab] copptby[WB] Traf6[Ab] is increased irt IL1 (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 10++ 20+ min
    *** source: 10882101(D)
  *** Traf6[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 1+ 2++ 5++ 10+ 20 min
    *** source: 11259596-Fig-5a
  *** Traf6[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (5 min)
    *** cells: HEK293 in BMS
    *** source: 11259596-Fig-7a
  *** Traf6[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3++ 15+ min
    *** source: 14633987-Fig-5a
  *** Traf6[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3++ 15+ min
    *** source: 14633987-Fig-6b
  *** Traf6[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293T<xIL1R1> in BMS
    *** times: 0 2+++ 5++ 10+ min
    *** comment: coppt Traf6 is ubiq[WBMS]
    *** comment: neither Traf6 ubiq or coppt is affected by addition of rUsp5 to the lysates
    *** source: 19675569-Fig-3b
  ---- requires Irak1 ??? --------------------
  *** Traf6[Ab] copptby[WB] Tab2[Ab] is unchanged irt IL1b (5 min)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** source: 11259596-Fig-7a

Tab3--

  *** Traf6[Ab] copptby[WB] Tab3[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3++ 15+ min
    *** source: 14633987-Fig-5a
  *** Traf6[Ab] copptby[WB] Tab3[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3++ 15+ min
    *** source: 14633987-Fig-6b

====================================================
Coprecipitation with other proteins in IL1R1 complex
====================================================

  *** Tak1[Ab] copptby[WB] Mekk3[Ab] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 5+ 10++ 20+ 30 min
    *** source: 19843958-Fig-3c

contradictions

  *** Tak1[Ab] copptby[WB] Mekk3[Ab] is unchanged irt IL1 (times)
    *** cells: HEK293 in BMS
    *** times: 0 5 10 30 min
    *** comment: no evidence that they could see Tak1
    *** source: 17197697-Fig-4f

==========================
Tab1 modifications irt IL1
==========================

  *** Tab1[Ab] phos[WBMS] is increased irt IL1b (30 min)
    *** cells: HEK293 in BMS
    *** source: 11518704-Fig-6b
  *** Tab1[Ab] phos[WBMS] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30++ min
    *** partially reqs: Tab2 [KO]
    *** source: 12556483-Fig-5
  *** Tab1[Ab]IP phos[WBMS-PPase] is increased irt IL1 (10 min)
    *** cells: HEK293<xIL1R1> in BMS
    *** source: 10702308-Fig-1

phos(S423)

  *** Tab1[phosAb] phos(S423)[phosAb] is increased irt IL1a (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 15+ 30+ min
    *** source: 18021073-Fig-6a
  *** Tab1[Ab]IP phos(S423)[phosAb] is increased irt IL1a (20 min)
    *** cells: KB in BMS
    *** inhibited by: SB203580 [addition] no-bkg-control
    *** unaffected by: PD184352 [addition] no-bkg-control
    *** source: 14592977-Fig-7
  *** Tab1[Ab]IP phos(S423)[phosAb] is increased irt IL1a (20 min)
    *** cells: mEFs in BMS
    *** source: 14592977-Fig-9c

phos(S438)

  *** Tab1[Ab]IP phos(S438)[phosAb] is increased irt IL1a (20 min)
    *** cells: mEFs in BMS
    *** source: 14592977-Fig-9c
  *** Tab1[phosAb] phos(S438)[phosAb] is increased irt IL1a (20 min)
    *** cells: KB in BMS
    *** unaffected by: SB203580 [addition] no-bkg-control
    *** inhibited by: PD184352 [addition] no-bkg-control
    *** source: 14592977-Fig-7
  *** Tab1[phosAb] phos(S438)[phosAb] is increased irt IL1a (20 min)
    *** cells: mEFs in BMLS
    *** source: 18021073-Fig-9a

phos(T431)

  *** Tab1[Ab]IP phos(T431)[phosAb] is increased irt IL1a (20 min)
    *** cells: KB in BMS
    *** inhibited by: SB203580 [addition] no-bkg-control
    *** unaffected by: PD184352 [addition] no-bkg-control
    *** source: 14592977-Fig-7
  *** Tab1[Ab]IP phos(T431)[phosAb] is increased irt IL1a (20 min)
    *** cells: mEFs in BMS
    *** source: 14592977-Fig-9c

==========================
Tab2 modifications irt IL1
==========================

  *** Tab2[Ab]IP phos[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3+ 10+ min
    *** source: 14633987-Fig-6b
  *** Tab2[Ab]IP phos[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3++ 15+ min
    *** source: 14633987-Fig-5a
  *** Tab2[Ab] phos[WBMS] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 15++ 30++ min
    *** source: 12556483-Fig-5
  *** Tab2[Ab] phos[WBMS] is increased irt IL1b (30 min)
    *** cells: HEK293 in BMS
    *** source: 11518704-Fig-6b
  *** Tab2[Ab]IP phos[WBMS] is increased irt IL1a (20 min)
    *** cells: mEFs in BMLS "immortalized"
    *** partially inhibited by: SB203580 [addition]
    *** source: 14670075-Fig-5a
  *** Tab2[Ab]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3+ 10+ min
    *** source: 14633987-Fig-6b
  *** Tab2[Ab]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: HEK293T<xIL1R1> in BMS
    *** times: 0 2+++ 5++ 10+ min
    *** comment: partially inhibited by addition of rUsp5 to the lysates
    *** source: 19675569-Fig-3b

==========================
Tab3 modifications irt IL1
==========================

  *** Tab3[Ab]IP phos[WBMS] is increased irt IL1a (20 min)
    *** cells: mEFs in BMLS "immortalized"
    *** inhibited by: SB203580 [addition]
    *** source: 14670075-Fig-5a
  *** Tab3[Ab]IP phos[WBMS] is increased irt IL1a (20 min)
    *** cells: mEFs in BMLS "immortalized"
    *** reqs: P38a [KO]
    *** source: 14670075-Fig-5b
  *** Tab3[Ab]IP phos[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3+ 10++ min
    *** source: 14633987-Fig-6b
  *** Tab3[Ab]IP phos[WBMS] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3++ 15+ min
    *** source: 14633987-Fig-5a
  *** Tab3[Ab]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3+ 10+ min
    *** source: 14633987-Fig-6b

==========================================
Tak1-Tab complex does not break up irt IL1
==========================================

  *** Tab2[Ab] copptby[WB] Tab1[Ab] is unchanged irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0+ 10+ 20+ 30+ min
    *** source: 18021073-Fig-3a
  *** Tab2[Ab] copptby[WB] Tab3[Ab] is unchanged irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0+ 3+ 15+ min
    *** comment: coppt Tab2 phos[WBMS] increases with time
    *** source: 14633987-Fig-5a
  *** Tab2[Ab] copptby[WB] Tak1[Ab] is unchanged irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0+ 3+ 15+ min
    *** comment: coppt Tab2 phos[WBMS] increases with time
    *** source: 14633987-Fig-5a

  *** Tab3[Ab] copptby[WB] Tab1[Ab] is unchanged irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0+ 10+ 20+ 30+ min
    *** source: 18021073-Fig-3a
  *** Tab3[Ab] copptby[WB] Tab2[Ab] is unchanged irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0+ 3+ 15+ min
    *** comment: coppt Tab3 phos[WBMS] increases with time
    *** source: 14633987-Fig-5a
  *** Tab3[Ab] copptby[WB] Tak1[Ab] is unchanged irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0+ 3+ 15+ min
    *** comment: coppt Tab3 phos[WBMS] increases with time
    *** source: 14633987-Fig-5a




















