=====================
Direct Ubiquitination
=====================

K48 links

  *** rTp53[35S] upshift[WBMS] is increased by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** inhibited by: rUbiq(dig)"no-lysines" [substitution]
    *** inhibited by: rUbiq(dig)"K6-only" [substitution]
    *** inhibited by: rUbiq(dig)"K11-only" [substitution]
    *** inhibited by: rUbiq(dig)"K27-only" [substitution]
    *** inhibited by: rUbiq(dig)"K29-only" [substitution]
    *** inhibited by: rUbiq(dig)"K33-only" [substitution]
    *** inhibited by: rUbiq(K63only) [substitution]
    *** partially inhibited by: rUbiq(dig)"K48-only" [substitution]
    *** source: 17110336-Fig-1d

required Tp53 features

  *** rTp53[35S] upshift[WBMS] is increased by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** unaffected by: rTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** inhibited by: rTp53(K319A/K320A/K321A) [substitution]
    *** source: 17110336-Fig-2a
  *** rTp53[35S] upshift[WBMS] is increased by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** unaffected by: rTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** unaffected by: rTp53(K370A/K372A/K373A) [substitution]
    *** unaffected by: rTp53(K381A/K382A/K386A) [substitution]
    *** source: 17110336-Fig-S3a
  *** rTp53[35S] upshift[WBMS] is increased by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** unaffected by: rTp53(del(309-330)) [substitution]
    *** source: 17110336-Fig-S3b
  *** rTp53[35S] upshift[WBMS] is increased by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** inhibited by: rTp53(mnr)"KEEK-mutant" [substitution]
    *** source: 17110336-Fig-S5c

required E4f1 features

  *** rTp53[35S] upshift[WBMS] is increased by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** inhibited by: rE4f1(del(41-85)) [substitution]
    *** source: 17110336-Fig-3c
  *** rTp53[Ab] upshift[WBMS] is increased by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** inhibited by: rE4f1(del(41-85)) [substitution]
    *** source: 17110336-Fig-7d

no extras

  *** rTp53[35S] upshift[WBMS] is increased by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** source: 17110336-Fig-1b
  *** rTp53[35S] upshift[WBMS] is increased by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** source: 17110336-Fig-3e
  *** rTp53[35S] upshift[WBMS] is increased by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** source: 17110336-Fig-S1a

=========================
Ubiquitinqtion by E4f1-IP
=========================

  *** rTp53[35S] upshift[WBMS] is increased by (xE4f1[Ab]IP + rUbe2d2 + rUbe1 + rUbiq)
    *** cells: none
    *** IPfrom: U2OS<xE4f1> in BMS
    *** source: 17110336-Fig-1c

=================================
Ubiquitinqtion in the presence of
=================================

required Tp53 features

  *** xTp53[Ab] ubiq[tUbAb] is increased itpo xE4f1
    *** cells: U2OS<xTp53><xUbiq> in BMS
    *** unaffected by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** inhibited by: xTp53(K319A/K320A/K321A) [substitution]
    *** source: 17110336-Fig-2c

required E4f1 features

  *** Tp53[Ab] ubiq[tUbAb] is increased itpo xE4f1
    *** cells: U2OS<xUbiq> in BMS
    *** inhibited by: xE4f1(del(41-85)) [substitution]
    *** source: 17110336-Fig-3d

no extras

  *** xTp53[Ab] ubiq[tUbAb-ppt] is increased itpo xE4f1
    *** cells: U2OS<xTp53><xUbiq> in BMS
    *** source: 17110336-Fig-S6b
  *** Tp53[Ab] ubiq[tUbAb] is decreased itao E4f1 [RNAi]
    *** cells: U2OS<xUbiq> in BMS
    *** source: 17110336-Fig-1g
  *** Tp53[Ab] ubiq[tUbAb] is increased itpo xE4f1
    *** cells: U2OS<xUbiq> in BMS
    *** source: 17110336-Fig-1f
  *** xTp53[Ab] ubiq[tUbAb] is increased itpo xUbiq
    *** cells: U2OS<xTp53> in BMS
    *** reqs: E4f1 [RNAi]
    *** source: 17110336-Fig-S2b
  *** xTp53[Ab] ubiq[tUbAb] is increased itpo xE4f1
    *** cells: U2OS<xTp53><xUbiq> in BMS
    *** does not req: Mdm2 [RNAi]
    *** source: 17110336-Fig-2d
  *** xTp53[Ab] ubiq[tUbAb] is increased itpo xE4f1
    *** cells: U2OS<xTp53><xUbiq> in BMS
    *** source: 17110336-Fig-S2c
  *** xTp53[Ab] ubiq[tUbAb] is increased itpo xE4f1
    *** cells: U2OS<xTp53><xUbiq> in BMS
    *** source: 17110336-Fig-S2f


=============================================================
E4f1 does not cause Tp53 degradation (evidence for K48 ubiq)
=============================================================

  *** Tp53[Ab] prot-exp[WB] is unchanged itpo xE4f1
    *** cells: U2OS in BMS "nuclear extracts"
    *** source: 17110336-Fig-1e
  *** Tp53[Ab] prot-stability[CHX-WB] is increased itpo xE4f1
    *** cells: U2OS in BMS
    *** source: 17110336-Fig-4a

===================
Protein Association
===================

  *** xE4f1[tAb] copptby[WB] xTp53[Ab] is detectable
    *** cells: U2OS<xTp53><xE4f1> in BMS
    *** unaffected by: xE4f1(del(41-85)) [substitution]
    *** source: 17110336-Fig-S4b
