=======================
Phosphorylation on S15
=======================

Atm-- ???

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (30 min)
    *** cells: GM9607<Atm~fmut><xAtm> in BMS
    *** inhibited by: xAtm(D2870A/N2875K)"KD" [substitution]
    *** inhibited by: xAtm(S1981A) [substitution]
    *** source: 12556884-Fig-2g
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (10 min)
    *** cells: AT1ABR<Atm~fmut><xAtm> in BMS
    *** reqs: xAtm [omission]
    *** unaffected by: xAtm(S367A) [substitution]
    *** partially inhibited by: xAtm(S1983A) [substitution]
    *** partially inhibited by: xAtm(S1981A) [substitution]
    *** source: 16858402-Fig-5b
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (times)
    *** cells: L3AT<Atm~fmut> in BMS
    *** times: 0 90+ 150++ 210++ min
    *** comment: no control run for total Tp53 protein
    *** comment: L3AT has a small amount of ATM protein in Fig-3a
    *** source: 15788536-Fig-1a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (times)
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS
    *** times: 0 10+ 60++ min
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 20026654-Fig-3

not Chek1--

  *** xTp53[phosAb] phos(S15)[phosAb] is increased irt IR (tnr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xChek1 [addition]
    *** unaffected by: xChek1(D130A) [addition]
    *** source: 10673501-Fig-4a

not Kdm6b-- Kdm4a-- Kdm4d--  ???

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (tnr)
    *** cells: HEK293T in BMS
    *** unaffected by: xKdm6b [addition]
    *** inhibited by: xKdm4a [addition]
    *** inhibited by: xKdm4d [addition]
    *** source: 19783983-Fig-4e

not Mdc1--

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 16377563-Fig-S5a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (times)
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS
    *** times: 0 10+ 60+ min
    *** does not req: Mdc1 [RNAi]
    *** source: 20026654-Fig-7a

Mre11--

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (15 min)
    *** cells: D6809<Mre11~fmut><xMre11> in BMS
    *** reqs: xMre11 [omission]
    *** unaffected by: xMre11(NLS) [substitution]
    *** source: 16478990-Fig-4c

not Nbs1-- ???

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (times)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** times: 0 15+ 30++ 60++ min
    *** does not req: xNbs1 [omission]
    *** unaffected by: xNbs1(1-734)"delC" [substitution]
    *** source: 15758953-Fig-3a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (15 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** partially reqs: xNbs1 [omission]
    *** inhibited by: xNbs1(1-652)"Nb652" [substitution]
    *** unaffected by: xNbs1(454-754)"NbFR5" [substitution]
    *** source: 16478990-Fig-3b
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (15 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** partially reqs: xNbs1 [omission]
    *** partially inhibited by: xNbs1(1-735)"dAtm" [substitution]
    *** unaffected by: xNbs1(454-754)"NbFR5" [substitution]
    *** inhibited by: xNbs1(454-735)"NbFR5sAtm" [substitution]
    *** source: 16478990-Fig-6b

not Smc1a--

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (15 min)
    *** cells: mEFs in BMS
    *** unaffected by: Smc1a(S957A/S966A) [knockin]
    *** source: 15175241-Fig-5b

Tp53 features

  *** xTp53[phosAb] phos(S15)[phosAb] is increased irt IR (2 hr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(S37A) [substitution]
    *** source: 10734067-Fig-5

no extras

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (times)
    *** cells: 1070SK in BMS
    *** times: 0 1+ 5+ hr
    *** source: 12556884-Fig-2d
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (times)
    *** cells: C3ABR in BMS
    *** times: 0 90+ 150+ 210+ min
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-1a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (times)
    *** cells: MCF7<xMdm4> in BMS
    *** times: 0 5+ 7++ hr
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-1b
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (15 min)
    *** cells: GM637 in BMS
    *** source: 16478990-Fig-3b
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (15 min)
    *** cells: GM637 in BMS
    *** source: 16478990-Fig-4c

===========
Acetylation
===========

  *** Tp53[Ab]IP acetyl(K320)[AcAb-ppt] is increased irt IR (4 hr)
    *** cells: U2OS in BMS
    *** source: 9891054-Fig-7c
  *** Tp53[Ab]IP acetyl(K373)[AcAb-ppt] is increased irt IR (4 hr)
    *** cells: U2OS in BMS
    *** source: 9891054-Fig-7c

===============================
Phosphorylation on other sites
===============================

not S20

  *** xTp53[phosAb] phos(S20)[phosAb] is detectable-but unchanged irt IR (2 hr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(S15A) [substitution] 
    *** source: 10734067-Fig-5

not T18

  *** xTp53[phosAb] phos(T18)[phosAb] is detectable-but unchanged irt IR (2 hr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** inhibited by: xTp53(S15A) [substitution] 
    *** unaffected by: xTp53(S37A) [substitution]
    *** source: 10734067-Fig-5


==================
Protein Expression
==================

does not req S15 or S37

  *** xTp53[Ab] prot-exp[WB] is unchanged irt IR (2 hr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(S15A) [substitution]
    *** unaffected by: xTp53(S37A) [substitution]
    *** source: 10734067-Fig-5

requires Atm--

  *** Tp53[Ab]IP prot-exp[WB] is unchanged irt IR (90 min)
    *** cells: GM1526<Atm~fmut> in BMS
    *** comment: Tp53 is not phosed on S15 [phosAb]
    *** source: 9733515-Fig-2b
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (1 hr)
    *** cells: mEFs in BMS
    *** reqs: Atm [KO]
    *** source: 9168116-Fig-1a
  *** Tp53[Ab] prot-exp[WB] is unchanged irt IR (times)
    *** cells: GM1526<Atm~fmut> in BMS
    *** times: 0+ 2+ 4+ hr
    *** source: 10766245-Fig-1b
  ------ expression is only delayed in this cell line ------
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (times)
    *** cells: GM2052<Atm~fmut> in BMS
    *** times: 0+ 30+ 60+ 120+ 180+ 240++ 360++ 600++ min
    *** source: 16931761-Fig-1c

not Nbs1--

  *** Tp53[Ab] prot-exp[WB] is unchanged irt IR (times)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** times: 0+ 15+ 30+ 60+ min
    *** unaffected by: xNbs1 [omission]
    *** unaffected by: xNbs1(1-734)"delC" [substitution]
    *** source: 15758953-Fig-3a

  *** Tp53[Ab] prot-exp[WB] is increased  irt IR (times)
    *** cells: A549 in BMS
    *** times: 0+ 15+ 30+ 45++ 60++ 120+++ 240+++ 480+++ 1440+++ min
    *** comment: new Tp53 is phosed on S15 [phosAb]
    *** comment: new Tp53 is phosed on T18 [phosAb]
    *** comment: new Tp53 is phosed on S20 [phosAb]
    *** source: 10734067-Fig-3
  *** xTp53[Ab] prot-exp[WB] is increased irt IR (2 hr)
    *** cells: MCF7<xTp53> in BMS
    *** source: 10673501-Fig-4b
  *** xTp53[Ab] prot-exp[WB] is increased irt IR (2 hr)
    *** cells: MCF7<xTp53><xChek1> in BMS
    *** source: 10673501-Fig-4b
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (3 hr)
    *** cells: LNCAP in BMS
    *** unaffected by: ALLN [cotreatment]
    *** source: 9363941-Fig-3a
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (3 hr)
    *** cells: RKO in BMS
    *** unaffected by: ALLN [cotreatment]
    *** source: 9363941-Fig-3a
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (times)
    *** cells: 70Z3 in BMS
    *** times: 0+ 1++ 2+++ 3+++ 4+++ hr
    *** partially inhibited by: Thapsigargin [pretreatment]
    *** source: 14744935-Fig-S1b
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (times)
    *** cells: DA1 in BMS
    *** times: 0 30+ 60++ 90++ 120+ 180+ min
    *** source: 9153395-Fig-4a
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (times)
    *** cells: LNCAP in BMS
    *** times: 0 30+ 60++ 120++ 180++ 240+ min
    *** source: 9363941-Fig-1
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (times)
    *** cells: MCF7 in BMS
    *** times: 0+ 7+ 15+ 30++ 45++ 60++ 120+++ 240+++ 480++ 720++ 1440++ min
    *** comment: new Tp53 protein is phosed on S15 [phosAb]
    *** comment: new Tp53 protein is phosed on S20 [phosAb]
    *** comment: new Tp53 protein is phosed on S26 [phosAb]
    *** source: 11030628-Fig-5b
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (times)
    *** cells: ML1 in BMS
    *** times: 0+ 1++ 2++ 3+++ 4++ 5+ hr 
    *** source: 9153395-Fig-4d
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (tnr)
    *** cells: U2OS in BMS
    *** source: 12397362-Fig-1b
  *** Tp53[Ab]IP prot-exp[WB] is increased irt IR (2 hr)
    *** cells: LNCAP in BMS
    *** comment: Tp53IP is phosed on S15 [phosAb]
    *** source: 9363941-Fig-2e
  *** Tp53[Ab]IP prot-exp[WB] is increased irt IR (2 hr)
    *** cells: RKO in BMS
    *** comment: Tp53IP is phosed on S15 [phosAb]
    *** source: 9363941-Fig-2d
  *** Tp53[Ab]IP prot-exp[WB] is increased irt IR (3 hr)
    *** cells: LNCAP in BMS
    *** unaffected by: ALLN [cotreatment]
    *** comment: Tp53 IP is phosed on S15 [phosAb]
    *** source: 9363941-Fig-3b
  *** Tp53[Ab]IP prot-exp[WB] is increased irt IR (3 hr)
    *** cells: RKO in BMS
    *** unaffected by: ALLN [cotreatment]
    *** comment: Tp53 IP is phosed on S15 [phosAb]
    *** source: 9363941-Fig-3b
  *** Tp53[Ab]IP prot-exp[WB] is increased irt IR (90 min)
    *** cells: GM2184 in BMS
    *** comment: Tp53 is phosed on S15 [phosAb]
    *** source: 9733515-Fig-2b
  *** Tp53[Ab]IP prot-exp[WB] is increased irt IR (times)
    *** cells: LNCAP in BMS
    *** times: 0 30+ 60++ 120++ 180+ 240 min
    *** comment: Tp53IP is phosed on S15 [phosAb]
    *** source: 9363941-Fig-2c
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (times)
    *** cells: GM536 in BMS
    *** times: 0+ 2++ 4++ hr
    *** comment: Tp53IP is phosed on S15 [phosAb]
    *** source: 10766245-Fig-1b
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (times)
    *** cells: GM2184 in BMS
    *** times: 0+ 2++ 4++ hr
    *** comment: Tp53IP is phosed on S15 [phosAb]
    *** source: 10766245-Fig-1b
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (15 min)
    *** cells: 1070SK in BMS
    *** comment: Tp53IP is phosed on S15 [phosAb]
    *** source: 15175241-Fig-1a
  *** Tp53[Ab] prot-exp[WB] is increased irt IR (times)
    *** cells: GM3490 in BMS
    *** times: 0 30+ 60++ 120+++ 180+++ 240+++ 360++ 600++ min
    *** source: 16931761-Fig-1a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt DSB-Induction (24 hr)
    *** cells: HEK293T in BMS
    *** times: 0 30 60+ 120+ 240+ 480+ 960++ 1440++ min
    *** source: 17486112-Fig-S1a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt IR (30 min)
    *** cells: HEK293T in BMS
    *** source: 17486112-Fig-S1a

???

  *** xTp53(S15A)[Ab] prot-exp[WB] is increased irt IR (75 min)
    *** cells: mEFs<Tp53~null><Atm~null><xTp53(S15A)> in BMS
    *** source: 10611322-Fig-1
  *** xTp53(S15A)[Ab] prot-exp[WB] is increased irt IR (75 min)
    *** cells: mEFs<Tp53~null><xTp53(S15A)> in BMS
    *** source: 10611322-Fig-1

  *** xTp53[Ab] prot-exp[WB] is detectable-but unchanged irt IR (tnr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** source: 10673501-Fig-4a
  *** Tp53[Ab] prot-exp[WB] is unchanged irt IR (15 min)
    *** cells: mEFs in BMS
    *** unaffected by: Smc1a(S957A/S966A) [knockin]
    *** source: 15175241-Fig-5b

==========================================================
Increase is due to stability not increase in transcription
==========================================================

  *** Tp53-gene mRNA[Northern] is unchanged irt IR (times)
    *** cells: ML1 in BMS
    *** times: 0 1+ 2+ 3+ 4+ 5+ hr 
    *** source: 9153395-Fig-4g



