======================================
Ctnnb1 degradation itpo Axin1 or Axin2
======================================

Axin1--

  *** Ctnnb1[Ab] prot-exp[WB] is decreased itpo xAxin1
    *** cells: Lcells in BMS
    *** source: 10698523-Fig-4b
  *** Ctnnb1[Ab]IP prot-exp[WB] is decreased itpo xAxin1
    *** cells: HEK293T<xBtrc> in BMS
    *** source: 10228155-Fig-1b
  *** Ctnnb1[Ab]IP prot-exp[WB] is decreased itpo xAxin1
    *** cells: HEK293T<xFbxw2> in BMS
    *** source: 10228155-Fig-1b

Axin2--

  *** xCtnnb1[tAb] prot-exp[WB] is decreased itpo xAxin2
    *** cells: SW480 in BMS
    *** reversed by: xAxin2(del(338-472)) [substitution]
    *** partially reversed by: xAxin2(mnr)"delRGS" [substitution] 
    *** source: 9554852-Fig-4a
  *** xCtnnb1(131-781)[tAb] prot-exp[WB] is detectable-but unchanged itpo xAxin2
    *** cells: SW480 in BMS
    *** source: 9554852-Fig-4a

  *** xCtnnb1[tAb] prot-stability[35S-pulse-chase] is decreased itpo xAxin1
    *** cells: HEK293T<xCtnnb1> in BMS
    *** unaffected by: xBtrc [addition]
    *** reversed by: xBtrc(del(140-194))"delFbox" [addition]
    *** source: 10228155-Fig-5


=============================
Ctnnb1 degradation itpo Gsk3b
=============================

  *** xCtnnb1[tAb]IP prot-exp[WB] is decreased itpo xGsk3b
    *** cells: HEK293T<xCtnnb1> in BMS
    *** source: 11967263-Fig-2a
  *** xCtnnb1[tAb]IP prot-exp[WB] is decreased itpo xGsk3b
    *** cells: HEK293T<xCtnnb1> in BMS
    *** source: 11967263-Fig-3a
  *** xCtnnb1[tAb] prot-exp[WB] is decreased itpo xGsk3b
    *** cells: HEK293<xCtnnb1> in BMS
    *** enhanced by: xPpp2r5a [addition]
    *** source: 10092233-Fig-2c

requires Ctnnb1 S45

  *** xCtnnb1[tAb]IP prot-exp[WB] is decreased itpo xGsk3b
    *** cells: HEK293T<xCtnnb1> in BMS
    *** reversed by: xCtnnb1(S45A) [substitution]
    *** source: 11967263-Fig-6a
  *** xCtnnb1[tAb]IP prot-exp[WB] is decreased itpo xGsk3b
    *** cells: HEK293T<xCtnnb1> in BMS
    *** reversed by: xCtnnb1(S45A) [substitution]
    *** source: 11967263-Fig-7

requires Gsk3b R96

  *** xCtnnb1[tAb]IP prot-exp[WB] is increased itpo xGsk3b(R96A)
    *** cells: HEK293T<xCtnnb1> in BMS
    *** source: 11967263-Fig-3a
  *** xCtnnb1[tAb]IP prot-exp[WB] is increased itpo xGsk3b(R96A)
    *** cells: HEK293T<xCtnnb1> in BMS
    *** source: 11967263-Fig-5

does not require Gsk3b kinase activity ???

  *** xCtnnb1[tAb]IP prot-exp[WB] is decreased itpo xGsk3b
    *** cells: HEK293T<xCtnnb1> in BMS
    *** enhanced by: xGsk3b(K85M/K86I)"KD" [substitution]
    *** source: 11967263-Fig-5

=======================================
Ctnnb1 degradation itpo Gsk3b and Axin1
=======================================

requires Ctnnb1 phos sites

  *** xCtnnb1[Ab] prot-exp[WB] is decreased itpo (xAxin1 + xGsk3b)
    *** cells: HEK293T<xCtnnb1> in BMS
    *** reversed by: xCtnnb1(S33A/S37A/T40A/S45A/S47A)"DP" [substitution]
    *** source: 12000790-Fig-1a
  *** xCtnnb1[Ab] prot-exp[WB] is decreased itpo (xAxin1 + xGsk3b)
    *** cells: HEK293T<xCtnnb1> in BMS
    *** unaffected by: xCtnnb1(S29F) [substitution]
    *** reversed by: xCtnnb1(S33Y) [substitution]
    *** reversed by: xCtnnb1(S37A) [substitution]
    *** reversed by: xCtnnb1(T41A) [substitution]
    *** reversed by: xCtnnb1(S45F) [substitution]
    *** source: 12000790-Fig-2b

inhibited by xDvl1--

  *** xCtnnb1[Ab] prot-exp[WB] is decreased itpo (xAxin1 + xGsk3b)
    *** cells: HEK293T<xCtnnb1> in BMS
    *** reversed by: xDvl1 [addition]
    *** source: 12000790-Fig-6b

does not req Axin1-Gsk3b direct binding

  *** xCtnnb1[Ab] prot-exp[WB] is decreased itpo (xAxin1 + xGsk3b)
    *** cells: HEK293T<xCtnnb1> in BMS "treated with MG132"
    *** unaffected by: xAxin1(L525P)"Gsk3b-binding-mut" [substitution]
    *** source: 12000790-Fig-3c

=============================
Endogenous Ctnnb1 degradation
=============================

requires Csnk1a1--

  *** Ctnnb1[Ab] prot-exp[WB] is increased itao Csnk1a1 [RNAi]
    *** cells: HEK293T in BMS
    *** source: 11955436-Fig-6d

unaffected by lack of Csnk1e--

  *** Ctnnb1[Ab] prot-exp[WB] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: Csnk1e [RNAi]
    *** source: 11955436-Fig-6e














  *** Ctnnb1[Ab] prot-exp[WB] is decreased itpo xBtrc
    *** cells: HELA in BMS
    *** reversed by: xBtrc(mnr)"delFbox" [substitution]
    *** source: 10074433-Fig-4a
  *** xCtnnb1[Ab] prot-exp[WB] is decreased itpo xPpp2r5a
    *** cells: HCA7<xCtnnb1> in BMS
    *** source: 10092233-Fig-3
  *** xCtnnb1[Ab] prot-exp[WB] is decreased itpo xPpp2r5a
    *** cells: HEK293<xCtnnb1> in BMS
    *** reversed by: ALLN [addition]
    *** source: 10092233-Fig-2b
  *** xCtnnb1[Ab] prot-exp[WB] is decreased itpo xPpp2r5a
    *** cells: HEK293<xCtnnb1> in BMS
    *** reversed by: xCtnnb1(mnr)"del90" [substitution]
    *** source: 10092233-Fig-2b
  *** xCtnnb1[Ab] prot-exp[WB] is decreased itpo xPpp2r5a
    *** cells: HEK293<xCtnnb1> in BMS
    *** source: 10092233-Fig-3
  *** Ctnnb1[Ab] prot-exp[WB] is increased itpo xApc(2026-2056)"Apc#4"
    *** cells: NEURO2A in BMS
    *** source: 9554852-Fig-4c
  *** Ctnnb1[Ab] prot-exp[WB] is increased itpo xWnt1
    *** cells: NIH3T3 in BMS "Cytosolic-fraction"
    *** enhanced by: xLrp5(1370-1615)"C2" [addition]
    *** partially inhibited by: xLrp5(1203-1615)"C1" [addition]
    *** source: 11336703-Fig-2b
  *** Ctnnb1[Ab] prot-exp[WB] is increased itpo xWnt1
    *** cells: NIH3T3 in BMS "Cytosolic-fraction"
    *** inhibited by: xLrp5(1-1372) [addition]
    *** inhibited by: xLrp5(1-300) [addition]
    *** source: 11336703-Fig-1d
  *** xCtnnb1[Ab] prot-exp[WB] is unchanged itpo xPpp2r5a
    *** cells: SW480<xCtnnb1> in BMS
    *** source: 10092233-Fig-3
  *** xCtnnb1[tAb] prot-exp[WB] is decreased itpo xPpp2r5a
    *** cells: HEK293<xCtnnb1> in BMS
    *** reversed by: MG132 [addition]
    *** source: 10092233-Fig-2a
  *** xCtnnb1[tAb] prot-exp[WB] is decreased itpo xPpp2r5a
    *** cells: HEK293<xCtnnb1> in BMS
    *** source: 10092233-Fig-1b
  *** xCtnnb1[tAb] prot-exp[WB] is decreased itpo xPpp2r5a
    *** cells: HEK293<xCtnnb1> in BMS
    *** source: 10092233-Fig-1c
  *** xCtnnb1[tAb] prot-exp[WB] is decreased itpo xPpp2r5b
    *** cells: HEK293<xCtnnb1> in BMS
    *** source: 10092233-Fig-1c
  *** xCtnnb1[tAb] prot-exp[WB] is decreased itpo xPpp2r5c1
    *** cells: HEK293<xCtnnb1> in BMS
    *** source: 10092233-Fig-1c
  *** xCtnnb1[tAb] prot-exp[WB] is decreased itpo xPpp2r5d
    *** cells: HEK293<xCtnnb1> in BMS
    *** source: 10092233-Fig-1c
  *** xCtnnb1[tAb] prot-exp[WB] is decreased itpo xPpp2r5e
    *** cells: HEK293<xCtnnb1> in BMS
    *** source: 10092233-Fig-1c
  *** xCtnnb1[tAb] prot-exp[WB] is unchanged itpo xPpp2r2a
    *** cells: HEK293<xCtnnb1> in BMS
    *** source: 10092233-Fig-1c

