===============
Kinase Activity
===============

chemicals

  *** S6k1[Ab]IP IVKA(Rps6)[32P-ATP] is increased irt Serum (15 min)
    *** cells: HEK293E in BMLS
    *** unaffected by: U0126 [pretreatment]
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** source: 17360704-Fig-1b
  *** xS6k1[tAb]IP IVKA(Eif4b)[32P-ATP] is increased irt Serum (15 min)
    *** cells: HELA<xS6k1> in BMLS
    *** inhibited by: Rapamycin [pretreatment]
    *** source: 16763566-Fig-4a

no extras

  *** S6k1[Ab]IP IVKA(snr)[32P-ATP] is increased irt Serum (times)
    *** cells: HEK293 in BMLS
    *** times: 0 5+ 15++ 30++ 60+++ 120++ min
    *** source: 9465032-Fig-2b
  *** S6k1[Ab]IP IVKA(snr)[32P-ATP] is increased irt Serum (times)
    *** cells: HEK293 in BMLS
    *** times: 0 5+++ 15++ 30+ 60 120 min
    *** source: 9405468-Fig-2b

S6k1 features

  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Serum (20 min)
    *** cells: HEK293<xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(T252A) [substitution]
    *** source: 9632736-Fig-1b
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Serum (20 min)
    *** cells: HEK293<xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(T412A) [substitution]
    *** bkg inhibited by: xS6k1(T252A) [substitution]
    *** bkg inhibited by: xS6k1(K123M) [substitution]
    *** unaffected by: xS6k1(S434A) [substitution]
    *** source: 9632736-Fig-1a
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Serum (30 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: xS6k1(mnr)"KD" [substitution]
    *** source: 11914378-Fig-6a
  *** xS6k1a2[Ab]IP IVKA(snr)[32P-ATP] is increased irt Serum (tnr)
    *** cells: HEK293<xS6k1a2> in BMLS
    *** inhibited by: xS6k1a2(T412A) [substitution]
    *** source: 9465032-Fig-3c

=====================
Total Phosphorylation
=====================

  *** S6k1[Ab] phos[WBMS] is increased irt Serum (times)
    *** cells: HEK293 in BMLS
    *** times: 0 5+ 15++ 30++ 30++ 60++ 120+ min
    *** source: 9405468-Fig-2b
  *** S6k1[Ab] phos[WBMS] is increased irt Serum (times)
    *** cells: HEK293 in BMLS
    *** times: 0 5+ 15++ 30++ 60+++ 120++ min
    *** source: 9465032-Fig-2b

===================================
Phosphorylation on T252 (CST T229)
===================================

  *** xS6k1[phosAb] phos(T252)[phosAb] is increased irt Serum (20 min)
    *** cells: HEK293<xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(T252A) [substitution]
    *** source: 9632736-Fig-1b

==================================
Phosphorylation on S394 (CST S371)
==================================

  *** xS6k1[phosAb] phos(S394)[phosAb] is unchanged irt Serum (30 min)
    *** cells: HEK293 in BMS
    *** source: 11914378-Fig-6a

==================================
Phosphorylation on T412 (CST T389)
==================================

Lamtor2--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (10 min)
    *** cells: mEFs in BMLS
    *** reqs: Lamtor2 [KO]
    *** source: 20381137-Fig-4a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (10 min)
    *** cells: mEFs<Lamtor1~null><xLamtor1> in BMLS
    *** reqs: xLamtor1 [omission]
    *** source: 20381137-Fig-4b

not Sin1--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (15 min)
    *** cells: mEFs in BMLS
    *** does not req: Sin1 [KO]
    *** source: 16962653-Fig-5a

Tsc2--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (90 min)
    *** cells: mEFs in BMLS
    *** reqs: Tsc2 [KO]
    *** source: 18198340-Fig-2a

reqs phos(T252)

  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (20 min)
    *** cells: HEK293<xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(T252A) [substitution]
    *** source: 9632736-Fig-1b

partially reqs phos(T434)

  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (20 min)
    *** cells: HEK293<xS6k1> in BMLS
    *** unaffected by: xS6k1(K123M) [substitution]
    *** partially inhibited by: xS6k1(T434A) [substitution]
    *** partially inhibited by: xS6k1(T444A/S447A) [substitution]
    *** source: 9632736-Fig-1a

chemicals

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (15 min)
    *** cells: HELA in BMLS
    *** unaffected by: FMK [pretreatment]
    *** source: 16763566-Fig-4e
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (30 min)
    *** cells: HELA in BMLS
    *** inhibited by: Rapamycin [addition]
    *** source: 15071500-Fig-5b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (30 min)
    *** cells: HELA in BMLS
    *** inhibited by: Rapamycin [pretreatment]
    *** unaffected by: U0126 [pretreatment]
    *** inhibited by: Rapamycin + U0126 [pretreatment]
    *** source: 16763566-Fig-1a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (30 min)
    *** cells: mESCs in BMLS
    *** inhibited by: Rapamycin [pretreatment]
    *** source: 16763566-Fig-3a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (30 min)
    *** cells: mESCs in BMLS
    *** inhibited by: Rapamycin [pretreatment]
    *** source: 16763566-Fig-3b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (30 min)
    *** cells: mHepatocytes in BMLS "AminoAcid and Glucose deprived"
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** source: 16763566-Fig-2a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (45 min)
    *** cells: mEFs<Tp53~null> in BMLS
    *** inhibited by: Rapamycin [pretreatment]
    *** source: 15545625-Fig-1a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (60 min)
    *** cells: mEFs in BMLS
    *** inhibited by: Aicar [cotreatment]
    *** inhibited by: Phenformin [cotreatment]
    *** source: 18439900-Fig-2a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (90 min)
    *** cells: mEFs<Ddit4~null> in BMLS
    *** bkg inhibited by: Rapamycin [cotreatment]
    *** unaffected by: Aicar [cotreatment]
    *** source: 15988001-Fig-2b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (times)
    *** cells: HEK293E in BMLS
    *** times: 0 5 10+ 30++ 60++ min
    *** unaffected by: U0126 [pretreatment]
    *** inhibited by: Rapamycin [pretreatment]
    *** source: 17360704-Fig-1a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (times)
    *** cells: HELA in BMLS
    *** times: 0 3 5 15+ 60+ 90+ min
    *** inhibited by: Rapamycin [pretreatment]
    *** unaffected by: U0126 [pretreatment]
    *** inhibited by: Rapamycin + U0126 [pretreatment]
    *** source: 16763566-Fig-1c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (times)
    *** cells: HELA in BMLS
    *** times: 0 1+ 3+ 6+ 12+ hr
    *** inhibited by: Rapamycin [cotreatment]
    *** inhibited by: Torin1 [cotreatment]
    *** source: 19446321-Fig-4e
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (tnr)
    *** cells: HELA in BMLS
    *** bkg inhibited by: Rapamycin [cotreatment] no-bkg-control
    *** inhibited by: MG132 [cotreatment] no-bkg-control
    *** inhibited by: Torin1 [cotreatment] no-bkg-control
    *** source: 19446321-Fig-4f

no extras

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (30 hr)
    *** cells: HEK293E in BMS
    *** source: 19446321-Fig-4c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (30 hr)
    *** cells: HELA in BMS
    *** source: 19446321-Fig-4c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (30 hr)
    *** cells: HT29 in BMS
    *** source: 19446321-Fig-4c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (30 hr)
    *** cells: MDAMB435 in BMS
    *** source: 19446321-Fig-4c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (times)
    *** cells: HELA in BMLS
    *** times: 0 1++ 3++ 6++ 9++ 12+ 14+ 20+ 24+ hr
    *** source: 19446321-Fig-4a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (times)
    *** cells: HELA in BMLS
    *** times: 0 3 5 10+ 15+ 30++ min
    *** source: 16763566-Fig-1b

not helpful

  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (30 min)
    *** cells: HEK293 in BMLS
    *** unaffected by: xS6k1(mnr)"KD" [substitution]
    *** source: 11914378-Fig-6a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (90 min)
    *** cells: mEFs<Ddit4~null> in BMLS
    *** unaffected by: xDdit4 [addition]
    *** source: 15988001-Fig-2b

????

  *** S6k1[phosAb] phos(T412)[phosAb] is decreased irt Serum-deprivation (24 hr)
    *** cells: RAT1A in BMS
    *** comment: total S6k1 prot-exp not reported
    *** source: 16027121-Fig-2f
  *** xS6k1[phosAb] phos(T412)[phosAb] is detectable-but unchanged irt Serum (20 min)
    *** cells: HEK293<xS6k1> in BMLS
    *** unaffected by: xS6k1(T412A) [substitution]
    *** unaffected by: xS6k1(T412E) [substitution]
    *** unaffected by: xS6k1(T252A) [substitution]
    *** unaffected by: xS6k1(K123M) [substitution]
    *** unaffected by: xS6k1(T444A/S447A) [substitution]
    *** source: 9632736-Fig-1a
  *** S6k1[phosAb] phos(T412)[phosAb] is detectable-but unchanged irt Serum (30 hr)
    *** cells: HEK293T in BMS
    *** source: 19446321-Fig-4c
  *** S6k1[phosAb] phos(T412)[phosAb] is detectable-but unchanged irt Serum (30 hr)
    *** cells: PC3 in BMS
    *** source: 19446321-Fig-4c
  *** S6k1[phosAb] phos(T412)[phosAb] is detectable-but unchanged irt Serum (30 hr)
    *** cells: U87MG in BMS
    *** source: 19446321-Fig-4c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Serum (1 hr)
    *** cells: HEK293T in BMLS
    *** bkg reqs: Mtor [RNAi]
    *** bkg reqs: Mlst8 [RNAi]
    *** bkg reqs: Raptor [RNAi]
    *** source: 12718876(D)
  *** S6k1[phosAb] phos(T412)[phosAb] is unchanged irt Serum (20 min)
    *** cells: mESCs in BMLS
    *** source: 11500364-Fig-S1d
  *** S6k1[phosAb] phos(T412)[phosAb] is unchanged irt Serum (90 min)
    *** cells: mEFs in BMLS
    *** does not req: Ddit4 [KO]
    *** source: 15988001-Fig-2a
  *** S6k1[phosAb] phos(T412)[phosAb] is unchanged irt Serum (90 min)
    *** cells: mEFs in BMLS
    *** bkg inhibited by: Rapamycin [cotreatment]
    *** unaffected by: Aicar [cotreatment]
    *** source: 15988001-Fig-2a

=======================
Phosphorylation on S434
=======================

reqs S6k1(phosT252)

  *** xS6k1[phosAb] phos(S434)[phosAb] is increased irt Serum (20 min)
    *** cells: HEK293<xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(T252A) [substitution]
    *** source: 9632736-Fig-1b

============================
Phosphorylation on T444/S447 (CST T421/S424)
============================

reqs S6k1(phosT252)

  *** xS6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Serum (20 min)
    *** cells: HEK293<xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(T252A) [substitution]
    *** source: 9632736-Fig-1b


  *** xS6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Serum (20 min)
    *** cells: HEK293<xS6k1> in BMLS
    *** unaffected by: xS6k1(T412A) [substitution]
    *** inhibited by: xS6k1(T412E) [substitution]
    *** partially inhibited by: xS6k1(T252A) [substitution]
    *** unaffected by: xS6k1(K123M) [substitution]
    *** unaffected by: xS6k1(T434A) [substitution]
    *** source: 9632736-Fig-1a

no extras

  *** S6k1[phosAb] phos(T444/T424)[phosAb] is increased irt Serum (20 min)
    *** cells: mESCs in BMLS
    *** source: 11500364-Fig-S1d

