===============
Phosphorylation
===============

Tsc2--

  *** Pras40[phosAb] phos(T246)[phosAb] is increased irt Ins (15 min)
    *** cells: mEFs in BMLS
    *** reqs: Tsc2 [KO]
    *** source: 17386266-Fig-3g

  *** Pras40[phosAb] phos(T246)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** source: 17386266-Fig-S1
  *** Pras40[phosAb] phos(T246)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** source: 17386266-Fig-3g
  *** Pras40[phosAb] phos(T246)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMLS
    *** source: 17386266-Fig-3g
  *** Pras40[phosAb] phos(T246)[phosAb] is increased irt Ins (10 min)
    *** cells: 3T3L1 in BMLS
    *** source: 17277771-Fig-3d
  *** xPras40[phosAb] phos(T246)[phosAb] is increased irt Ins (10 min)
    *** cells: 3T3L1<xPras40> in BMLS
    *** source: 17277771-Fig-3d
  *** Pras40[phosAb] phos(T246)[phosAb] is increased irt Ins (10 min)
    *** cells: HEK293T in BMLS
    *** bkg reversed by: Wortmannin [pretreatment]
    *** source: 17277771-Fig-4b
  *** xPras40[tAb]IP phos(T246)[phosAb] is increased irt Ins (20 min)
    *** cells: HEK293T<xPras40> in BMLS
    *** bkg inhibited by: Wortmannin [pretreatment]
    *** source: 17277771-Fig-S4a

================================
decrease in coppt with Raptor???
================================

  *** Pras40[Ab] copptby[WB] Raptor[Ab] is decreased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** comment: bkg coppt is decreased with high salt wash
    *** source: 17386266-Fig-3b
  *** Pras40[Ab] copptby[WB] Raptor[Ab] is decreased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** source: 17386266-Fig-3c
  *** Pras40[Ab] copptby[WB] Raptor[Ab] is decreased irt Ins (15 min)
    *** cells: HELA in BMLS
    *** source: 17386266-Fig-3g
  *** Pras40[Ab] copptby[WB] Raptor[Ab] is unchanged irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** source: 17386266-Fig-3g
  *** Pras40[Ab] copptby[WB] Raptor[Ab] is unchanged irt Ins (15 min)
    *** cells: mEFs in BMLS
    *** source: 17386266-Fig-3g
  *** Pras40[Ab] copptby[WB] xRaptor[tAb] is decreased irt Ins (15 min)
    *** cells: HEK293E<xRaptor> in BMLS
    *** source: 17386266-Fig-3d

================================
decrease in coppt with Mtor???
================================

  *** Pras40[Ab] copptby[WB] Mtor[Ab] is decreased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** enhanced by: Rapamycin [pretreatment]
    *** reversed by: Wortmannin [pretreatment]
    *** unaffected by: U0126 [pretreatment]
    *** source: 17510057-Fig-3c
  *** Pras40[Ab] copptby[WB] Mtor[Ab] is decreased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** source: 17510057-Fig-3b
  *** Pras40[Ab] copptby[WB] Mtor[Ab] is decreased irt Ins (times)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** times: 0+ 5 10 30 min
    *** source: 17510057-Fig-3a
  *** Pras40[Ab] copptby[WB] Mtor[Ab] is decreased irt Ins (20 min)
    *** cells: HEK293T in BMLS
    *** bkg reversed by: Wortmannin [pretreatment]
    *** source: 17277771-Fig-4b


  *** Mtor[Ab] snaggedby[WB] rPras40[GST] is increased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** bkg inhibited by: xPras40(F129A) [addition]
    *** source: 17510057-Fig-S3c
  *** Raptor[Ab] snaggedby[WB] rPras40[GST] is increased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** bkg inhibited by: xPras40(F129A) [addition]
    *** source: 17510057-Fig-S3c













