===============
Kinase Activity
===============

Cdc42--

  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (10 min)
    *** cells: NIH3T3 in BMLS
    *** inhibited by: xCdc42(T17N)"DN" [addition]
    *** source: 8653792-Fig-2a,3

Mlst8-- Mtor-- Raptor--  Rapamycin only

  *** S6k1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt Egf (times)
    *** cells: RAT2 in BMS
    *** times: 0 2+ 5++ 10++ 20++ 40++ min
    *** inhibited by: Rapamycin [addition]
    *** source: 11297520-Fig-5c

not Mekk3--

  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (30 min)
    *** cells: NIH3T3 in BMLS
    *** unaffected by: xMekk3(mnr)"DN" [addition]
    *** source: 8653792-Fig-4c

Rac1--  or Cdc42???

  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (10 min)
    *** cells: NIH3T3 in BMLS
    *** partially inhibited by: xRac1(T17N)"DN" [addition]
    *** source: 8653792-Fig-2a,3

S6k1 features

  *** xS6k1[tAb]IP IVKA(S6peptide)[32P-ATP] is increased irt Egf (20 min)
    *** cells: COS7<xS6k1> in BMLS
    *** inhibited by: xS6k1(R121A) [substitution]
    *** source: 12374740-Fig-8a

no extras

  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 10++ 30+ min
    *** source: 8653792-Fig-4a
  *** S6ks[Ab]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (times)
    *** cells: SWISS3T3 in BMLS
    *** times: 0 5+++ 10++ 15++ 20++ 30++ 45+ 60+ 75+ 90+ 120+ 240+ min
    *** source: 1848664-Fig-2

=======================
Phosphorylation on T412
=======================

Gnai1-- and/or Gnai3--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (15 min)
    *** cells: mEFs in BMLS
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-1a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2 5+ 15++ 30++ min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-1b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gnai1~null><Gnai3~null><xGnai1> in BMLS
    *** times: 0 10+ 20+ min
    *** reqs: xGnai1 [omission]
    *** source: 19401591-Fig-3b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gnai1~null><Gnai3~null><xGnai3> in BMLS
    *** times: 0 10+ 20+ min
    *** reqs: xGnai3 [omission]
    *** source: 19401591-Fig-3b

not Ikk2--

  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Egf (30 min)
    *** cells: MCF7<xS6k1> in BMLS
    *** does not req: Ikk2 [RNAi]
    *** source: 17693255-Fig-3f

Pi3k-- chemicals only

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15++ min
    *** inhibited by: LY294002 [addition]
    *** source: 16984645-Fig-4a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15++ min
    *** inhibited by: LY294002 [addition]
    *** source: 16984645-Fig-4b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS
    *** times: 0 7+ 15+ min
    *** inhibited by: LY294002 [addition]
    *** source: 16984645-Fig-5
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15+ min
    *** inhibited by: LY294002 [addition]
    *** source: 16984645-Fig-4c

not Mek12s--  chemicals only

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15+ min
    *** unaffected by: PD98059 [addition]
    *** source: 16984645-Fig-4c

Raptor-- Rapamycin only

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15++ min
    *** inhibited by: Rapamycin [addition]
    *** source: 16984645-Fig-4a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15++ min
    *** inhibited by: Rapamycin [addition]
    *** source: 16984645-Fig-4b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS
    *** times: 0 7+ 15+ min
    *** inhibited by: Rapamycin [addition]
    *** source: 16984645-Fig-5

chemicals

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 20+ min
    *** unaffected by: PTX [pretreatment]
    *** source: 19401591-Fig-3e

no extras

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (30 min)
    *** cells: MCF7 in BMLS
    *** source: 17693255-Fig-3d
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Egf (30 min)
    *** cells: mEFs<Ikk2~null> in BMLS
    *** source: 17693255-Fig-3e

==============================
Phosphorylation on other sites
==============================

S6k1 features

  *** xS6k1[tAb]IP phos(T252)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xS6k1> in BMLS
    *** unaffected by: xS6k1(R121A) [substitution]
    *** source: 12374740-Fig-8b

chemicals

  *** S6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS
    *** times: 0 7+ 15+ min
    *** partially inhibited by: LY294002 [addition]
    *** source: 16984645-Fig-5
  *** S6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS
    *** times: 0 7+ 15+ min
    *** partially inhibited by: Rapamycin [addition]
    *** source: 16984645-Fig-5

no extras

  *** S6k1[phosAb] phos(T412/T444/S447)[phosAb] is increased irt Egf (times)
    *** cells: MDAMB361 in BMLS
    *** times: 0 5+ 10++ 20++ 30++ 45+ 60+ min
    *** source: 11297548(D)

================
coppt with Eif3b
================

  *** S6k1[Ab] copptby[WB] Eif3b[Ab] is decreased irt Egf (30 min)
    *** cells: HEK293E in BMLS
    *** unaffected by: Rapamycin [addition]
    *** source: 16286006-Fig-1d


