===========================================
A bit of contradiction - need more evidence
===========================================

=============
Ubiqutination
=============

  *** xSmad2[tAb] copptby[WB] xUbiq[tAb] is increased itpo xSmurf2
    *** cells: COS1 in BMS
    *** inhibited by: xSmurf2(C716G)"CIA" [substitution]
    *** source: 11158580-Fig-3d
  *** xSmad2[tAb]IP ubiq[tUbAb] is increased itpo xSmurf2
    *** cells: HEK293<xUbiq> in BMS "treated with MG132"
    *** inhibited by: xSmurf2(C716A)"CIA" [substitution]
    *** source: 11016919-Fig-3a

===========
Degradation ????
===========

  *** xSmad2[tAb] prot-exp[WB] is decreased itpo xSmurf2
    *** cells: HEK293 in BMS
    *** inhibited by: xSmurf2(C716A)"CIA" [substitution]
    *** source: 11016919-Fig-3b
  *** xSmad2[tAb] prot-exp[WB] is decreased itpo xSmurf2
    *** cells: HEK293 in BMS
    *** source: 11016919-Fig-2
  *** xSmad2[tAb] prot-exp[WB] is increased irt MG132 (tnr)
    *** cells: HEK293<xSmurf2> in BMS
    *** source: 11016919-Fig-3b
  --------------------------------------------------------------
  *** xSmad2[tAb] prot-exp[WB] is decreased itpo xSmurf2
    *** cells: COS1 in BMS
    *** inhibited by: Lactacystin [addition]
    *** source: 11158580-Fig-3b
  *** xSmad2[tAb] prot-exp[WB] is decreased itpo xSmurf2
    *** cells: COS1 in BMS
    *** inhibited by: xSmurf2(C716G)"CIA" [substitution]
    *** source: 11158580-Fig-3a
  *** xSmad2[tAb] prot-exp[WB] is decreased itpo xSmurf2
    *** cells: HEK293T in BMS
    *** source: 11389444-Fig-2c
  ----------------------------------------------------------------
  *** xSmad2[tAb] prot-exp[WB] is unchanged itpo xSmurf2
    *** cells: HEK293T in BMS
    *** source: 11163210-Fig-1a
  *** xSmad2[Ab] prot-exp[WB] is unchanged itpo xSmurf2
    *** cells: HEK293T in BMS
    *** unaffected by: xTgfbR1(mnr)"CA" [addition]
    *** source: 11389444-Fig-2b

=================
Protein Stability
=================

  *** xSmad2[tAb]IP prot-stability[35S-pulse-chase] is unchanged itpo xSmurf2
    *** cells: HEK293T in BMS
    *** unaffected by: xTgfbR1(mnr)"CA" [addition]
    *** source: 11389444-Fig-2c

=====
Coppt
=====

  *** xSmad2[tAb] copptby[WB] xSmurf2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xSmurf2(C716A)"CIA" [substitution]
    *** source: 11016919-Fig-3c
  *** xSmad2[tAb] copptby[WB] xSmurf2[tAb] is undetectable
    *** cells: HEK293T in BMS
    *** source: 11163210-Fig-1a
  *** xSmad2[tAb] copptby[WB] xSmurf2[tAb] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xSmurf2(1-37)"C2-domain" [substitution]
    *** enhanced by: xSmurf2(248-369)"WW-domain" [substitution]
    *** inhibited by: xSmurf2(363-748)"HECT-domain" [substitution]
    *** source: 11158580-Fig-2c
  *** xSmurf2(248-369)[tAb] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 11158580-Fig-2a
  *** xSmurf2[tAb] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 11158580-Fig-2a
  *** xSmurf2[tAb] copptby[WB] xSmad2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xSmurf2(C716A)"CIA" [substitution]
    *** source: 11016919-Fig-3c
  *** xSmad2[tAb] copptby[WB] xSmurf2(mnr)"CIA"[tAb] is detectable
    *** cells: HEK293T<xSkil><xTgfbR1(mnr)"CA"> in BMS
    *** inhibited by: xSmad2(del(221-225))"del-PY" [substitution]
    *** source: 11389444-Fig-6b

===================
Direct Interactions
===================

  *** xSmad2[Ab] snaggedby[WB] rSmurf2(244-434)[GST] is detectable
    *** cells: HACAT in BMS
    *** comment: snagged xSmad2 is phosed(S465/S467)[phosAb] only irt Tgfb1 (4 hr)
    *** source: 11016919-Fig-4
  *** rSmurf2[35S] boundby[WB] rSmad2[GST] is detectable
    *** cells: none
    *** source: 11016919-Fig-1b

