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Phosphorylation on Tyrosine
===========================

reqs Src--

  *** xNckipsd[tAb]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: mEFs<Src~null><Fyn~null><xNckipsd><xVav2><xSrc> in BMS
    *** reqs: xSrc [omission]
    *** source: 14765113-Fig-1f

Required features

  *** xNckipsd[tAb]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: COS7<xNckipsd> in BMS
    *** unaffected by: xNckipsd(58-722)"dSH3" [substitution]
    *** inhibited by: xNckipsd(del(170-249))"dPro" [substitution]
    *** unaffected by: xNckipsd(mnr)"dCterm" [substitution]
    *** source: 14765113-Fig-2a
  *** xNckipsd[tAb]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: COS7<xNckipsd><xVav2> in BMS "membrane-fractions"
    *** inhibited by: xNckipsd(del(170-249))"dPro" [substitution]
    *** inhibited by: xNckipsd(Y161F) [substitution]
    *** unaffected by: xNckipsd(Y227F) [substitution]
    *** inhibited by: xNckipsd(Y161F/Y227F) [substitution]
    *** source: 14765113-Fig-2d

Effect of other treatments

  *** xNckipsd[tAb]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: NIH3T3<xDiaph1><xNckipsd><xSrc> in BMS "membrane-fractions"
    *** enhanced by: LPA [cotreatment]
    *** source: 14765113-Fig-4b

Chemicals

  *** xNckipsd[tAb]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: NIH3T3<xDiaph1><xNckipsd><xSrc> in BMS "membrane-fractions"
    *** inhibited by: PP1 [pretreatment]
    *** source: 14765113-Fig-4b

No extras

  *** Nckipsd[Ab]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: COS7 in BMS
    *** source: 14765113-Fig-1b
  *** xNckipsd[tAb]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: COS7<xNckipsd> in BMS
    *** source: 14765113-Fig-1a
  *** xNckipsd[tAb]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: COS7<xNckipsd> in BMS
    *** times: 0 5+ 10+ 30 min
    *** source: 14765113-Fig-1e
