===============
Kinase Activity
===============

inhibited by Dok2--

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: COS1 in BMS
    *** times: 0 2+ 5++ 10++ min
    *** partially inhibited by: xDok2 [addition]
    *** source: 10508618-Fig-3a

inhibited by Dusp1--

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xDusp1 [addition]
    *** source: 10473620-Fig-4c

inhibited by Dusp6--

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xDusp6 [addition]
    *** source: 10473620-Fig-4d

EgfR-- phos sites

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293<xEgfR> in BMLS
    *** unaffected by: xEgfR(Y1148F) [substitution]
    *** unaffected by: xEgfR(Y1173F) [substitution]
    *** unaffected by: xEgfR(Y954F/Y1153F) [substitution]
    *** source: 9733788-Fig-5f

reqs Hras--

  *** xErk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: mEFs in BMLS
    *** inhibited by: xHras(S17N)"DN" [addition]
    *** source: 11524436-Fig-3a
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7<xErk2> in BMLS
    *** partially inhibited by: xHras(S17N) [addition]
    *** source: 10330169-Fig-1c

Gabs--

Requirement for Gab1 phos(Y627/Y659) or Gab2 phos(Y614/Y643)
for Erk activation
~/evidence/Egf-Evidence/196.Gabs.txt

reqs IqGap1--

  *** Erks[phosAb]IP IVKA(Elk1)(sitenr)[phosAb] is increased irt Egf (10 min)
    *** cells: MCF7 in BMS
    *** partially inhibited by: xIqGap1 [addition]
    *** bkg reqs: IqGap1 [RNAi]
    *** source: 14970219-Fig-4a

reqs Mek1--

  *** xErk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: REF52 in BMS
    *** partially inhibited by: xMek1(K97A)"KD" [addition]
    *** source: 10480934-Fig-3d
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xMek1(mnr)"DN" [addition]
    *** source: 10473620-Fig-4e

not Mek5--

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xMek5(S311A/T315A)"DN" [addition]
    *** source: 10473620-Fig-4e

not Mekk1--

  *** Erk2[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS in BMLS
    *** inhibited by: xMekk1(K1274M)"DN" [addition] no-bkg-control
    *** source: 9305638-Fig-4e

not Mekk2--

  *** Erk2[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS in BMLS
    *** unaffected by: xMekk2(K385M)"DN" [addition]
    *** source: 9305638-Fig-4e

not Mekk3--

  *** Erk2[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS in BMLS
    *** unaffected by: xMekk3(K391M)"DN" [addition]
    *** source: 9305638-Fig-4e

not Mekk4--

  *** Erk2[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS in BMLS
    *** unaffected by: xMekk4(K1371M)"DN" [addition]
    *** source: 9305638-Fig-4e

not Mkk4--

  *** xErk1[tAb]IP IVKA(auto)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xMkk4(S220A/T224L)"DN" [addition]
    *** source: 12738796-Fig-3b


inhibited by Mlk3--  ???   scaffold???

  *** xErk1[tAb]IP IVKA(auto)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7<xErk1> in BMLS
    *** inhibited by: xMlk3 [addition]
    *** source: 12738796-Fig-3b
  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7<xErk1> in BMLS
    *** inhibited by: xMlk3 [addition]
    *** source: 12738796-Fig-3a
  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7<xErk1> in BMLS
    *** inhibited by: xMlk3 [addition]
    *** source: 12738796-Fig-1a

reqs Pi3k--

  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xPik3r?(mnr)"DN" [addition] no-bkg-control
    *** partially inhibited by: xPik3ca(K802R)"KD" [addition] no-bkg-control
    *** partially inhibited by: xPik3cb(K805R)"KD" [addition] no-bkg-control
    *** source: 11134009-Fig-2a
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7<xErk2> in BMLS
    *** bkg inhibited by: xPik3r?(mnr)"DN" [addition]
    *** source: 10330169-Fig-1b

not Pten--

  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xPten [addition] no-bkg-control
    *** unaffected by: xPten(G129E) [addition] no-bkg-control
    *** source: 11134009-Fig-3

inhibited by Ptpn6--

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293<xEgfR> in BMLS
    *** inhibited by: xPtpn6 [addition]
    *** source: 9733788-Fig-5f

does not req Raf1--

  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: mEFs in BMLS
    *** does not req: Raf1 [KO]
    *** unaffected by: kRaf1(Y340F/Y341F) [substitution]
    *** source: 11296227-Fig-5d
  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2++ 5++ 10++ 20++ 60+ min
    *** does not req: Raf1 [KO]
    *** unaffected by: kRaf1(Y340F/Y341F) [substitution]
    *** source: 11296227-Fig-5c

not RapGef1--

  *** xErk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0+ 5++ 10+ 20+ 30+ 40+ 60+ min
    *** unaffected by: xRapGef1(mnr)"Crk-binding-mut" [addition]
    *** source: 9560161-Fig-5b

RasGrf1-- ???

  *** xErk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xRasGrf1 [addition]
    *** source: 11285260-Fig-1c

reqs Shc1--  ???

  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HELA in BMS
    *** enhanced by: xShc1p52 + xShc1p46 [addition]
    *** unaffected by: xShc1p66(1-110)"CH2-domain" [addition]
    *** source: 9049300-Fig-9b

not Sos1--

  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: mEFs<xEgfR> in BMLS
    *** times: 0 5++ 15++ 90 360 min
    *** does not req: Sos1 [KO]
    *** source: 10675333-Fig-7c
  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: mEFs<xEgfR> in BMLS
    *** does not req: Sos1 [KO]
    *** source: 10675333-Fig-6b
  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** unaffected by: xSos1 [addition]
    *** source: 11134009-Fig-4d

reqs Shoc2--  ???

  *** xErk1[tAb]IP IVKA(Elk1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** enhanced by: xShoc2 [addition]
    *** source: 10783161-Fig-1c

Tec--  ???

  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xTec(mnr)"PHDomain" [addition] no-bkg-control
    *** unaffected by: xTec(mnr/R29C)"PIP3-binding-mutant" [addition] no-bkg-control
    *** source: 11134009-Fig-3

Usp6nl--  ????

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: COS7 in BMS
    *** times: 0 15++ 30+ min
    *** unaffected by: xUsp6nl [addition]
    *** source: 11099046-Fig-4b

Erk2 features

  *** xErk2[tAb]IP IVKA(Fos)[32P-ATP] is increased irt Egf (5 min)
    *** cells: NIH3T3<xErk2> in BMLS
    *** inhibited by: xErk2(L198A) [substitution]
    *** inhibited by: xErk2(L232A) [substitution]
    *** inhibited by: xErk2(Y261A) [substitution]
    *** unaffected by: xErk2(D319N) [substitution]
    *** source: 16051177-Fig-1c
  *** xErk2[tAb]IP IVKA(Rsk?)[32P-ATP] is increased irt Egf (5 min)
    *** cells: NIH3T3<xErk2> in BMLS
    *** unaffected by: xErk2(L198A) [substitution]
    *** unaffected by: xErk2(L232A) [substitution]
    *** unaffected by: xErk2(Y261A) [substitution]
    *** partially inhibited by: xErk2(D319N) [substitution]
    *** source: 16051177-Fig-1b

Chemicals

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7<xErk2> in BMLS
    *** bkg inhibited by: LY294002 [pretreatment]
    *** source: 10330169-Fig-1b
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7<xErk2> in BMLS
    *** partially inhibited by: LY294002 [pretreatment]
    *** source: 10330169-Fig-1c

No extras

  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: MDCK in BMLS
    *** times: 0 5++ 15++ 30+ 60+ 120+ 180 240 min
    *** source: 9668053-Fig-1b

=====================
Total Phosphorylation
=====================

  *** xErk2[tAb]IP phos[WBMS] is increased irt Egf (10 min)
    *** cells: HEK293T in BMLS
    *** source: 8631302-Fig-5
  *** Erks[Ab] phos[WBMS] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: LY294002 [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 10330169-Fig-1a



============================================================
requirement for Gab1 phos(Y627/Y659) or Gab2 phos(Y614/Y643)
============================================================

on Y627 (Gab1) or Y614 (Gab2)

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** partially inhibited by: xGab1(Y627F)"DN" [addition]
    *** source: 10788507-Fig-4c
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** partially inhibited by: xGab1(Y627F)"DN" [addition]
    *** source: 10788507-Fig-3c
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** partially inhibited by: xGab1(Y627F)"DN" [addition]
    *** source: 11323411-Fig-2b
  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** unaffected by: xGab1 [addition]
    *** inhibited by: xGab1(Y627F) [addition]
    *** source: 11134009-Fig-7c
  ----------------------------------------------------------------
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: T47D in BMLS
    *** partially inhibited by: xGab2(Y614F)"DN" [addition]
    *** source: 15952937-Fig-2a
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** partially inhibited by: xGab2(Y614F)"DN" [addition]
    *** source: 15952937-Fig-2b

on Y659

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xGab1(Y659F)"DN" [addition]
    *** source: 11323411-Fig-2b

on Y627 and Y659 (Gab1) Y614 and Y643 (Gab2)

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: MDAMB468 in BMLS
    *** inhibited by: xGab1(Y627F/Y659F) [addition]
    *** source: 14665621-Fig-8e
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** bkg inhibited by: xGab1(Y627F/Y659F) [addition]
    *** source: 11323411-Fig-2b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gab1~null><xGab1> in BMLS
    *** times: 0 10++ 30+ min
    *** partially inhibited by: xGab1(Y627F/Y659F) [substitution]
    *** source: 12629518-Fig-4d
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: T47D in BMLS
    *** inhibited by: xGab1(Y627F/Y659F)"DN" [addition]
    *** source: 15952937-Fig-2a
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xGab1(Y627F/Y659F)"DN" [addition]
    *** source: 15952937-Fig-2b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: rHepatocytes in BMLS
    *** unaffected by: xGab2(Y603F/Y632F) [addition]
    *** comment: xGab2 is from rat, human sites are Y614F/Y643F
    *** source: 12464621-Fig-8d

not Y447F or Y472F or Y589F

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** unaffected by: xGab1(Y447F/Y472F/Y589F)"Pi3k-binding-mut" [addition]
    *** source: 10788507-Fig-4c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gab1~null><xGab1> in BMLS
    *** times: 0 10++ 30+ min
    *** unaffected by: xGab1(Y447F/Y472F/Y589F) [substitution]
    *** source: 12629518-Fig-4d

not in PH domain

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** unaffected by: xGab1(del(10-177))"PH-domain-mut" [addition]
    *** source: 10788507-Fig-4c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gab1~null><xGab1> in BMLS
    *** times: 0 10++ 30+ min
    *** unaffected by: xGab1(del(14-116))"PH-domain-mut" [substitution]
    *** source: 12629518-Fig-4d
  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** unaffected by: xGab1 [addition]
    *** unaffected by: xGab1(104-694)"PH-domain-mutant" [addition]
    *** source: 11134009-Fig-7c

not Pro-Rich domain

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gab1~null><xGab1> in BMLS
    *** times: 0 10++ 30+ min
    *** partially inhibited by: xGab1(del(449-531)) [substitution]
    *** source: 12629518-Fig-4d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: rHepatocytes in BMLS
    *** unaffected by: xGab2(del(348-355)) [addition]
    *** comment: xGab2 is from rat, human sites are (351-358)
    *** source: 12464621-Fig-6d

modifications unknown

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5+++ 10+++ 30++ 90+ min
    *** partially reqs: Gab1 [KO]
    *** source: 12629518-Fig-1d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5++ 15++ 30+ min
    *** partially reqs: Gab1 [KO]
    *** source: 12629518-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gab1~null><xGab1> in BMLS
    *** times: 0 10++ 30+ min
    *** partially reqs: xGab1 [omission]
    *** source: 12629518-Fig-4d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: rHepatocytes in BMLS
    *** enhanced by: xGab2 [addition]
    *** source: 12464621-Fig-8d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: rHepatocytes in BMLS
    *** enhanced by: xGab2 [addition]
    *** source: 12464621-Fig-6d


  *** Erk1[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293 in BMS
    *** source: 9079650-Fig-5
  *** Erk2[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293 in BMS
    *** source: 9079650-Fig-5
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7 in BMS
    *** source: 8910292-Fig-2
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: NIH3T3 in BMLS
    *** source: 9006902-Fig-2b
  *** xErk2[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS1 in BMS
    *** source: 8940179-Fig-5
  *** Erks[Ab] Yphos[pYAb-ppt] is increased irt Egf (times)
    *** cells: HELA in BMLS
    *** times: 0 1+ 5++ 10++ 20+ min
    *** source: 15314609-Fig-2
  *** Erk2[MS] Yphos[pYAb-ppt] is increased irt Egf (times)
    *** cells: HELA in BMLS
    *** times: 0 1 5++ 10++ 20 min
    *** source: 15314609-Table-1
  *** xErk2[tAb]IP phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 2++ 5+++ 10++ 15+ min
    *** source: 12167697-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 10+ 30+ 60 120 min
    *** source: 14751248-Fig-2
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMLS
    *** times: 0 1+ 3++ 5+++ 10+++ 30++ 60+ min
    *** source: 11923843-Fig-S2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** source: 14963038-Fig-7b
  *** xErk2[tAb]IP phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: COS in BMLS
    *** times: 0 1 2+ 5++ 10++ 20+ min
    *** comment: response is delayed by 5 min itpo xAbi1
    *** source: 11003655-Fig-4b
  *** xErk2[tAb]IP phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: COS in BMLS
    *** times: 0 20++ 40+ 60+ min
    *** source: 11003655-Fig-4c
  *** xErk2[tAb]IP phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: COS in BMLS
    *** source: 11003655-Fig-4a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 15++ 30+ 60+ 180 min
    *** source: 12049732-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 5++ 30++ 60+ 120+ 240 480 min
    *** source: 16775008-Fig-4d
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: PC12 in BMLS
    *** source: 9094716-Fig-2d
  *** Erk1[Ab]IP IVKA(Elk1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: bLUCs in BMLS
    *** source: 12644307-Fig-5a
  *** Erk2[Ab]IP IVKA(Elk1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: bLUCs in BMLS
    *** source: 12644307-Fig-5a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0 2++ 5+ 10 30 60 min
    *** source: 11466412-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 5+ 10+ 30+ min
    *** source: 14613929-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: U87MG in BMLS
    *** source: 14701753-Fig-2a,2b
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS1 in BMLS
    *** source: 12594221-Fig-1b
  *** xErk2[tAb]IP IVKA(Elk1)(S383)[phosAb] is increased irt Egf (10 min)
    *** cells: COS1 in BMLS
    *** source: 12594221-Fig-3
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: SWISS3T3 in BMLS
    *** times: 0 5+ 45 60 90 120 300 min
    *** source: 12134156-Fig-6a
  *** xErk1[tAb]IP phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: COS7 in BMLS
    *** source: 12738796-Fig-2e
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: BETAHC9 in BMS
    *** times: 0 2+ 5+ 15+ min
    *** source: 15192046-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: CHO<xEgfR><xInsR> in BMS
    *** times: 0 2+ 5+ 15+ min
    *** source: 15192046-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: MDAMB361 in BMLS
    *** times: 0 5+ 10+ 20+ 30+ 45+ 60+ min
    *** source: 11297548-Fig-2
  *** Erk1[Ab]IP IVKA(Rsk1)[32P-ATP] is increased irt Egf (times)
    *** cells: SWISS3T3 in BMLS
    *** times: 0 5++ 10++ 60 90 120 240 360 min
    *** comment: phosphorylated Erks are located in the nucleus [IHC]
    *** source: 12134156-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** source: 11896055-Fig-6b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMLS
    *** times: 0 2+ 10+ min
    *** source: 16041367-Fig-3a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: PC6 in BMLS
    *** source: 15632082-Fig-1c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (tnr)
    *** cells: MEWO in BMLS
    *** source: 19274086-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (tnr)
    *** cells: B16F1 in BMLS
    *** source: 19274086-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is unchanged irt Egf (tnr)
    *** cells: 3731T in BMLS
    *** source: 19274086-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: A431 in BMS
    *** source: 17646016-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: A4318a in BMS
    *** source: 17646016-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (30 min)
    *** cells: HACAT in BMS
    *** source: 17085434-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is unchanged irt Egf (30 min)
    *** cells: HACAT in BMS "nuclear-extracts"
    *** comment: no total Smad1 controls were performed
    *** source: 17085434-Fig-1c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMS
    *** times: 0 1+ 2++ 5++ 10++ 15+ 20+ 30+ 45 60 120 min
    *** source: 12191474-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (30 min)
    *** cells: HACAT in BMLS
    *** source: 15241418-Fig-S5b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (30 min)
    *** cells: MV1LU in BMLS
    *** source: 16156666-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: MV1LU in BMLS
    *** times: 0 5+++ 15++ 30++ 60++ 120+ 240+ 480+ hr
    *** source: 16156666-Fig-1d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (tnr)
    *** cells: MV1LU in BMLS
    *** inhibited by: U0126 [addition] no-bkg-control
    *** inhibited by: PD98059 [addition] no-bkg-control
    *** source: 16156666-Fig-2
  *** Erks[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (15 min)
    *** cells: MV1LU in BMLS
    *** source: 10601313-Fig-1b
  *** Erks[Ab]IP IVKA(Smad3)[32P-ATP] is increased irt Egf (tnr)
    *** cells: MV1LU in BMLS
    *** source: 10601313-Fig-4a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: NIH3T3<xEgfR><xShp2(R465E)"DN"> in BMLS
    *** times: 0 2++ 10++ 60+ 240 min
    *** source: 14560030-Fig-8a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: HEK293T in BMLS
    *** source: 11598209-Fig-7c
  *** xErk2[tAb]IP phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** source: 11278335-Fig-6b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0 2+ 5+++ 15++ 30 60 min
    *** source: 11278445-Fig-3c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: PC12 in BMLS
    *** source: 11733534-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: SWISS3T3 in BMLS
    *** times: 0 10+ 60 90 120 240 360 min
    *** source: 14673150-Fig-3c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: 3Y1<xEgfR> in BMS
    *** times: 0 5++ 15++ 30++ 60++ 120 240 360 min
    *** source: 11134345-Fig-8a
  *** xErk2[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xErk2> in BMLS
    *** source: 10480933-Fig-7
  *** Erks[phosAb] phos(TPY)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 5+ 10+ 20+ 30+ min
    *** source: 19393267-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMS
    *** times: 0 10++ 30+ 60+ min
    *** source: 19687304-Fig-3a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 1 3+ 5+ 7+ 10+ 15+ 20+ 30+ min
    *** source: 19766101-Fig-2b



======================================================
Effect of chemicals on Erk Phosphorylation on TEY site
======================================================

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (15 min)
    *** cells: 3Y1 in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** source: 15024089-Fig-4a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (15 min)
    *** cells: HEK293E in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** source: 15024089-Fig-4a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (1 hr)
    *** cells: REF52 in BMS
    *** inhibited by: U0126 [addition]
    *** source: 10480934-Fig-4b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: RAT2 in BMS
    *** inhibited by: U0126 [addition]
    *** source: 11297520-Fig-6b
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: U0126 [addition] no-bkg-control
    *** source: 10473620-Fig-4a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (30 min)
    *** cells: NR6<xEgfR> in BMLS
    *** inhibited by: U0126 [addition] no-bkg-control
    *** source: 14747473-Fig-6b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: CHO<xEgfR><xInsR> in BMS
    *** inhibited by: U0126 [addition]
    *** source: 15192046-Fig-6
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (15 min)
    *** cells: mEFs<Jnk1~null><Jnk2~null> in BMLS
    *** inhibited by: U0126 [addition]
    *** source: 12110590-Fig-3d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (1 hr)
    *** cells: REF52 in BMS
    *** inhibited by: PD98059 [addition]
    *** source: 10480934-Fig-4b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: hUVECs in BMLS
    *** inhibited by: PD98059 [addition]
    *** source: 10671553-Fig-9a,9b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: RAT2 in BMS
    *** inhibited by: PD98059 [addition]
    *** source: 11297520-Fig-6b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: RAT2 in BMS
    *** inhibited by: PD98059 [addition]
    *** source: 11297520-Fig-6b
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: PD98059 [addition] no-bkg-control
    *** source: 10473620-Fig-4a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: HEK293T in BMLS
    *** inhibited by: PD98059 [addition]
    *** source: 11598209-Fig-7b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** inhibited by: PD184352 [addition] no-bkg-control
    *** source: 11909979-Fig-3a
  *** xErk2[tAb]IP phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 2+ 5++ 10+++ min
    *** inhibited by: PP1 [addition]
    *** source: 15831486-Fig-1,2
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: MDAMB468 in BMLS
    *** inhibited by: PP1 [addition]
    *** source: 14665621-Fig-8a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (15 min)
    *** cells: NIH3T3<xInsR> in BMLS
    *** partially inhibited by: PP1 [addition]
    *** source: 11046144-Fig-6b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (15 min)
    *** cells: mEFs<Jnk1~null><Jnk2~null> in BMLS
    *** unaffected by: PP1 [addition]
    *** source: 12110590-Fig-6a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (2 min)
    *** cells: A7R5 in BMLS
    *** unaffected by: PP2 [addition]
    *** source: 11907028-Fig-6a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: H197 in BMS
    *** unaffected by: Rottlerin [pretreatment]
    *** source: 10330161-Fig-2b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: H197 in BMS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 10330161-Fig-2d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: H197 in BMS
    *** unaffected by: PDB [pretreatment]
    *** unaffected by: Go6976 [pretreatment]
    *** unaffected by: Rottlerin [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** unaffected by: Chelerythrine [pretreatment]
    *** inhibited by: PD98059 [pretreatment]
    *** source: 10330161-Fig-3b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: PC12 in BMS
    *** unaffected by: Rottlerin [pretreatment]
    *** unaffected by: PDB [pretreatment]
    *** source: 10330161-Fig-5b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: NIH3T3 in BMS
    *** unaffected by: Rottlerin [pretreatment]
    *** source: 10330161-Fig-6a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (30 min)
    *** cells: HEK293<xTsc2> in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** unaffected by: Wortmannin [pretreatment] no-bkg-control
    *** source: 15851026-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: mESCs in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** source: 11018520-Fig-2c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: A431 in BMS
    *** bkg inhibited by: AG1478 [addition]
    *** bkg inhibited by: Compound-56 [addition]
    *** unaffected by: CGP77675 [addition]
    *** source: 17646016-Fig-3b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (3 min)
    *** cells: C3H10T12 in BMLS
    *** unaffected by: Bisindolylmaleimide-I [addition]
    *** unaffected by: Go6976 [addition]
    *** source: 11358964-Fig-3b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (20 min)
    *** cells: HELA in BMLS
    *** inhibited by: PD153035 [addition]
    *** source: 9790194-Fig-1d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: PD98059 [pretreatment] no-bkg-control
    *** source: 1118451-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** source: 1118451-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (30 min)
    *** cells: 3Y1<xEgfR> in BMS
    *** inhibited by: 1Butanol [pretreatment]
    *** unaffected by: isoButanol [pretreatment]
    *** source: 11134345-Fig-8b
  *** Erks[phosAb] phos(TEY)[phosAb] is detectable
    *** cells: MDAMB468 in BMLS "treated with Egf for 5 hr"
    *** inhibited by: Gefitinib [pretreatment]
    *** inhibited by: AG1478 [pretreatment]
    *** unaffected by: AG490 [pretreatment]
    *** unaffected by: LY294002 [pretreatment]
    *** inhibited by: U0126 [pretreatment]
    *** source: 17909010-Fig-3a


Bisindolylmaleimide-I--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: hUVECs in BMLS
    *** unaffected by: Bisindolylmaleimide-I [addition]
    *** source: 10671553-Fig-9a,9b

Forskolin--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (15 min)
    *** cells: HELA in BMLS
    *** unaffected by: Forskolin + IBMX [addition]
    *** source: 16581779-Fig-4c
  *** Erk1[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: PC12 in BMLS
    *** unaffected by: Forskolin [addition]
    *** source: 9094716-Fig-2a

H89--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: RAT2 in BMS
    *** inhibited by: H89 [addition]
    *** source: 11297520-Fig-7a

LY294002--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: COS in BMS
    *** times: 0 2+ 5+ 10+ 20+ 60+ min
    *** partially inhibited by: LY294002 [addition]
    *** source: 11259591-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (30 min)
    *** cells: NR6<xEgfR> in BMLS
    *** unaffected by: LY294002 [addition]
    *** source: 14747473-Fig-6d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15+ min
    *** unaffected by: LY294002 [addition]
    *** source: 16984645-Fig-4a
  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: LY294002 [addition] no-bkg-control
    *** source: 11134009-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: LY294002 [addition] no-bkg-control
    *** source: 11134009-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: LY294002 [pretreatment]
    *** source: 10330169-Fig-1a

PD180970--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: MDAMB468 in BMLS
    *** unaffected by: PD180970 [addition]
    *** source: 14665621-Fig-8c

PD184352--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: RAT2 in BMS
    *** times: 0 2+ 5+ 10+ 20+ 40 min
    *** bkg inhibited by: PD184352 [addition]
    *** source: 11297520-Fig-6a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: RAT2 in BMS
    *** inhibited by: PD184352 [addition]
    *** source: 11297520-Fig-6b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: RAT2 in BMS
    *** inhibited by: PD184352 [addition]
    *** source: 11297520-Fig-6b

PMA--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: COS7 in BMLS
    *** times: 0 5+ 10+ min
    *** unaffected by: PMA [addition]
    *** source: 11283245-Fig-1d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** unaffected by: PMA [addition]
    *** source: 11283245-Fig-7b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (3 min)
    *** cells: C3H10T12 in BMLS "pretreated overnight with PMA"
    *** source: 11358964-Fig-3a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (3 min)
    *** cells: C3H10T12 in BMLS "pretreated overnight with PMA"
    *** unaffected by: Bisindolylmaleimide-I [addition]
    *** unaffected by: Go6976 [addition]
    *** source: 11358964-Fig-3b

Rapamycin--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: RAT2 in BMS
    *** unaffected by: Rapamycin [addition]
    *** source: 11297520-Fig-6b

Ro318220--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: RAT2 in BMS
    *** unaffected by: Ro318220 [addition]
    *** source: 11297520-Fig-6b

SB203580--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (15 min)
    *** cells: mEFs<Jnk1~null><Jnk2~null> in BMLS
    *** unaffected by: SB203580 [addition]
    *** source: 12110590-Fig-3d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** unaffected by: SB203580 [addition] no-bkg-control
    *** source: 11909979-Fig-3a

SU416--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: hUVECs in BMLS
    *** unaffected by: SU5416 [addition]
    *** source: 10671553-Fig-9a,9b


Wortmannin--

  *** Erk2[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS in BMLS
    *** unaffected by: Wortmannin [addition] no-bkg-control
    *** source: 9305638-Fig-4b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: hUVECs in BMLS
    *** unaffected by: Wortmannin [addition]
    *** source: 10671553-Fig-9a,9b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: COS in BMS
    *** times: 0 2+ 5+ 10+ 20+ 60+ min
    *** partially inhibited by: Wortmannin [addition]
    *** source: 11259591-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (10 min)
    *** cells: RAT2 in BMS
    *** unaffected by: Wortmannin [addition]
    *** source: 11297520-Fig-6b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1+ 3++ 5++ 10+ 30+ min
    *** partially inhibited by: Wortmannin [addition]
    *** source: 16687399-Fig-2b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1 3+ 4++ 5++ 7+++ 10++ 15+ 30+ min
    *** partially inhibited by: Wortmannin [addition]
    *** source: 16687399-Fig-2d
  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** source: 11134009-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** source: 11134009-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** source: 11134009-Fig-1c
