==================
Relocation to ssDNA
==================

Atm--

  *** Atr[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: hFibros<Atm~fmut><xAtm> in BMS
    *** reqs: xAtm [omission]
    *** source: 16327781-Fig-2a

Nbs1--

  *** Atr[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: hFibros<Nbs1~fmut><xNbs1> in BMS
    *** reqs: xNbs1 [omission]
    *** unaffected by: xNbs1(S278A/S343A/S397A) [substitution]
    *** source: 16327781-Fig-2b

no extras

  *** xAtr[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (10 min)
    *** cells: U2OS<xAtr> in BMS
    *** comment: limited to cells expressing CyclinA
    *** source: 16327781-Fig-6a
  *** xAtr[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS<xAtr> in BMS
    *** times: 0 1 2 4+ 6++ 10++ 15++ min
    *** source: 16327781-Fig-5a
  *** xAtr[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (14 min)
    *** cells: U2OS<xAtr> in BMS
    *** comment: increase was monitored continuously for 14 min after MicroLaser
    *** comment: increase was slower than xNbs1 xMdc1 xRnf8 xBrca1 xTp53bp1 in same system
    *** source: 18001824-Fig-3b
  *** xAtr[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS<xAtr> in BMS
    *** source: 16618811-Fig-9a
  *** xAtr[XFP] locatedin(subnuclear-bodies)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS<xAtr> in BMS
    *** comment: subnuclear bodies are smaller than those occupied by xMdc1 xTp53bp1 xRad52
    *** source: 16618811-Fig-9b

==============
Colocalization
==============

  *** xAtr[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (10 min)
    *** cells: U2OS<xAtr> in BMS
    *** comment: colocates with H2ax{phos(S140)} [phosAb]
    *** comment: colocates with Chek1{phos(S317)} [phosAb]
    *** source: 16327781-Fig-2d
  *** xAtr[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS<xAtr> in BMS
    *** comment: sublocalizes with H2ax-phos(S140)
    *** source: 16618811-Fig-3


=============================
Located in chromatin fraction
=============================

  *** Atr[Ab] infraction(chromatin)[WB] is increased irt IR (45 min)
    *** cells: NotReported in BMS
    *** source: 16461339-Fig-2b
  *** Atr[Ab] infraction(chromatin)[WB] is increased irt IR (45 min)
    *** cells: RAJI in BMS
    *** unaffected by: Wortmannin [pretreatment]
    *** source: 16461339-Fig-2a
  *** Atr[Ab] infraction(chromatin)[WB] is unchanged irt IR (45 min)
    *** cells: NotReported<Atm~fmut> in BMS
    *** source: 16461339-Fig-2b
  *** Atr[Ab] locatedin(nucleus)[IHC] is increased irt IR (45 min)
    *** cells: NotReported in BMS
    *** comment: cells were extracted with detergent before fixation so Atr not bound to chromatin was lost
    *** comment: nuclear staining is speckled
    *** source: 16461339-Fig-3a
  *** Atr[Ab] locatedin(nucleus)[IHC] is unchanged irt IR (45 min)
    *** cells: NotReported<Atm~fmut> in BMS
    *** comment: cells were extracted with detergent before fixation so Atr not bound to chromatin was lost
    *** comment: nuclear staining is not visible
    *** source: 16461339-Fig-3a

