============================================================
Proteins required for kinase activity or phophosphorylation
============================================================

not Abl1--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgfb (times)
    *** cells: mEFs<Abl1~null><Abl2~null><xAbl1> in BMLS
    *** times: 0 3+ 30++ min
    *** does not req: xAbl1 [omission]
    *** source: 15039778-Fig-S2a

Bad-- ???

  *** Akt1[Ab]IP IVKA(Bad)[32P-ATP] is increased irt Pdgfb (tnr)
    *** cells: BALB3T3 in BMLS
    *** unaffected by: rBad(S75A) [substitution]
    *** inhibited by: rBad(S99A) [substitution]
    *** inhibited by: rBad(S75A/S99A) [substitution]
    *** source: 9346240-Fig-2a

not Gab1--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgfb (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ min
    *** does not req: Gab1 [KO]
    *** source: 19401591-Fig-4a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgfb (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ min
    *** does not req: Gab1 [KO]
    *** source: 19401591-Fig-4a

not Gnai1-- or Gnai3--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgfb (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 20+ min
    *** does not req: Gnai1 or Gnai3 [KO]
    *** source: 19401591-Fig-2a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgfb (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 20+ min
    *** does not req: Gnai1 or Gnai3 [KO]
    *** source: 19401591-Fig-2a

not Freud1--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (10 min)
    *** cells: HEK293T in BMLS
    *** does not req: Freud1 [RNAi]
    *** source: 18662999-Fig-5a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgf (10 min)
    *** cells: HEK293T in BMLS
    *** does not req: Freud1 [RNAi]
    *** source: 18662999-Fig-5a

Sin1--  for phos(S473) but not phos(T308)

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (15 min)
    *** cells: mEFs in BMLS
    *** reqs: Sin1 [KO]
    *** source: 16962653-Fig-5b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgf (15 min)
    *** cells: mEFs in BMLS
    *** does not req: Sin1 [KO]
    *** source: 16962653-Fig-5b

Pdpk1--

  *** xAkt1(S473D)[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Pdgfb (1 min)
    *** cells: PAE<xAkt1(S473D)><xPdk1> in BMLS
    *** partially inhibited by: xPdpk1(R474A) [substitution]
    *** source: 9637919-Fig-5

Rictor--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (times)
    *** cells: mEFs in BMS
    *** times: 0 5+ 10+ 30+ min
    *** reqs: Rictor [KO]
    *** source: 16962829-Fig-4e
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs in BMLS
    *** partially reqs: Rictor [KO]
    *** source: 21464307-Fig-4b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs in BMLS
    *** partially reqs: Rictor [KO]
    *** source: 21464307-Fig-4b
  ---- only partially required
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs<Rictor~null> in BMLS
    *** source: 21464307-Fig-S4a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs<Rictor~null> in BMLS
    *** source: 21464307-Fig-S4a


not Traf2--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (15 min)
    *** cells: mEFs in BMS
    *** does not req: Traf2 [KO]
    *** source: 16260598-Fig-4c

not Traf6--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (15 min)
    *** cells: mEFs in BMS
    *** does not req: Traf6 [KO]
    *** source: 16260598-Fig-6e

Tbk1--  ???

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs<Rictor~null><xTbk1> in BMLS
    *** partially reqs: xTbk1 [omission]
    *** source: 21464307-Fig-4b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (30 min)
    *** cells: mEFs in BMLS
    *** partially reqs: Ikke and/or Tbk1 [KO]
    *** source: 21464307-Fig-2b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgf (30 min)
    *** cells: mEFs in BMLS
    *** does not req: Ikke or Tbk1 [KO]
    *** source: 21464307-Fig-2b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs<Rictor~null><xTbk1> in BMLS
    *** partially reqs: xTbk1 [omission]
    *** source: 21464307-Fig-4b

=========
Chemicals
=========

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMS
    *** times: 0 2+ 5+ 10+ 20+ 60+ min
    *** inhibited by: Wortmannin [addition]
    *** inhibited by: LY294002 [addition]
    *** source: 11259591-Fig-2a
  *** Akt1[Ab]IP IVKA(Bad)[32P-ATP] is increased irt Pdgfb (tnr)
    *** cells: BALB3T3 in BMLS
    *** inhibited by: Wortmannin [pretreatment]
    *** inhibited by: LY294002 [pretreatment]
    *** source: 9346240-Fig-1b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs in BMLS
    *** inhibited by: Torin1 [pretreatment]
    *** source: 21464307-Fig-4b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs<Rictor~null> in BMLS
    *** unaffected by: Torin1 [pretreatment]
    *** source: 21464307-Fig-S4b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs<Rictor~null><xTbk1> in BMLS
    *** unaffected by: Torin1 [pretreatment]
    *** source: 21464307-Fig-4b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs<Rictor~null><xTbk1> in BMLS
    *** unaffected by: Torin1 [pretreatment]
    *** source: 21464307-Fig-4b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs in BMLS
    *** unaffected by: Torin1 [pretreatment]
    *** source: 21464307-Fig-4b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Pdgf (20 min)
    *** cells: mEFs<Rictor~null> in BMLS
    *** unaffected by: Torin1 [pretreatment]
    *** source: 21464307-Fig-S4b

=========
no extras
=========

  *** Akt1[phosAb] phos(S473/T308)[phosAb] is increased irt Pdgf (5 min)
    *** cells: NIH3T3 in BMLS
    *** source: 11340168-Fig-1b
  *** xAkt1[tAb]IP IVKA(HistH3)[32P-ATP] is increased irt Pdgf (10 min)
    *** cells: NIH3T3 in BMLS
    *** source: 8653792-Fig-5d
  *** xAkt1(S473D)[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Pdgfb (1 min)
    *** cells: PAE<xAkt1(S473D)> in BMLS
    *** source: 9637919-Fig-4a
  *** xAkt1(S473D)[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Pdgfb (1 min)
    *** cells: PAE<xAkt1(S473D)> in BMLS
    *** source: 9637919-Fig-7
  *** xAkt1(S473D)[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Pdgfb (1 min)
    *** cells: PAE<xAkt1(S473D)><xPdk1> in BMLS
    *** source: 9637919-Fig-7


