================================
Expressed Mekk1 is kinase active
================================

  *** xMekk1[tAb]IP IVKA(Mkk4)[32P-ATP] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xMekk1(mnr)"DN" [substitution]
    *** source: 9819420-Fig-1a

Kinase Activity of expressed Mekk1 requires D1530 T1391 T1393

  *** xMekk1[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMekk1(D1530N) [substitution]
    *** inhibited by: xMekk1(T1391A) [substitution]
    *** inhibited by: xMekk1(T1393A) [substitution]
    *** source: 11784851(D)

===============================
Expressed Mekk1 is oligomerized
===============================

  *** xMekk1[tAb] oligomerization[2TagPpt] is detectable
    *** cells: HEK293 in BMS
    *** source: 11784851(D)
  *** xMekk1[tAb] oligomerization[density-gradient] is detectable
    *** cells: HEK293 in BMS
    *** source: 11784851(D)

=================================
Expressed Mekk1 is phosphorylated
=================================

  *** xMekk1[tAb]IP phos[32Pi] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xPpp2cb [addition]
    *** unaffected by: xPpp2cb(R179G)"phosphatase-defective-mut" [addition]
    *** source: 14585847-Fig-2b

=========================================
Endogenous Mekk1 is phosphorylated on S67
=========================================

  *** Mekk1[phosAb] phos(S67)[phosAb] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: Tnf + IL1 + Egf (tnr) [stim]
    *** source: 12228228-Fig-4a

====================================================
Coprecipitations, Snags, Binding, and Colocalization
====================================================

Crk--

  *** Mekk1[Ab] snaggedby[WB] Crk[GST] is undetectable
    *** cells: CCL39<xEgfR> in BMS
    *** source: 9733714-Fig-1a

Erk1--

  *** xMekk1[Ab] copptby[WB] xErk1[Ab] is detectable
    *** cells: HEK293T in BMLS
    *** source: 12049732-Fig-4b

Erk2--

  *** rMekk1[Ab] boundby[WB] rErk2[GST] is detectable
    *** cells: none
    *** source: 12049732-Fig-2c
  *** Mekk1[Ab] colocwith[IHC] Erk2[Ab] is detectable
    *** cells: HEK293T in BMLS
    *** unaffected by: Sorbitol (1 hr) [stim]
    *** source: 12049732-Fig-2d

Gadd45b--

  *** xMekk1[tAb] copptby[WB] xGadd45b[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** source: 14743220-Fig-1a

Grb2--

  *** Mekk1[Ab] copptby[WB] Grb2[Ab] is detectable
    *** cells: CCL39<xEgfR> in BMS
    *** source: 9733714-Fig-1b
  *** Mekk1[Ab] snaggedby[WB] Grb2[GST] is detectable
    *** cells: CCL39<xEgfR> in BMS
    *** source: 9733714-Fig-1a
  *** xMekk1[tAb] copptby[WB] Grb2[Ab] is detectable
    *** cells: CCL39<xEgfR> in BMS
    *** source: 9733714-Fig-1b

Ikk1--

  *** xMekk1(352-1514)"CA"[tAb] copptby[WB] xIkk1[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 9819420-Fig-4a

Ikk2--

  *** xMekk1(352-1514)"CA"[tAb] copptby[WB] xIkk2[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 9819420-Fig-4a

Jnk1--

  *** xMekk1[Ab] copptby[WB] xJnk1[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 9405400-Fig-4a

Map4k4--

  *** xMekk1[tAb] copptby[WB] xMap4k4[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 9135144-Fig-7a

Mapk6--

  *** xMekk1[tAb] copptby[WB] Mapk6[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 10969079-Fig-7b

Sin1--

  *** xMekk1[tAb] copptby[WB] xSin1[tAb] is undetectable
    *** cells: COS1 in BMS
    *** source: 15988011-Fig-2b

Mekk4--

  *** Mekk1[Ab] copptby[WB] Mekk4[Ab] is undetectable
    *** cells: HEK293T in BMS
    *** source: 12878610-Fig-3

Mkk6--

  *** xMekk1[tAb] snaggedby[WB] rMkk6[GST] is undetectable
    *** cells: COS1 in BMS
    *** source: 9808624-Fig-4a

Mkk7--

  *** xMekk1[tAb] snaggedby[WB] rMkk7[GST] is undetectable
    *** cells: COS1 in BMS
    *** source: 9808624-Fig-4a


Nck1--

  *** Mekk1[Ab] snaggedby[WB] Nck1[GST] is undetectable
    *** cells: CCL39<xEgfR> in BMS
    *** source: 9733714-Fig-1a

P38s--

  *** xMekk1[Ab] copptby[WB] xP38?[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** source: 9405400-Fig-4a

Pak1--

  *** xMekk1[tAb] copptby[WB] xPak1[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** source: 10969079-Fig-1

Raf1--

  *** xMekk1[tAb] copptby[WB] xRaf1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMekk1(D1369A)"KD" [substitution]
    *** unaffected by: xRaf1(S259D) [substitution]
    *** unaffected by: xRaf1(del(66-303)) [substitution]
    *** source: 10969079-Fig-1,2

Ripk1--

  *** xMekk1[tAb] copptby[WB] Ripk1[Ab] is undetectable
    *** cells: HEK293T in BMS
    *** source: 14585994-Fig-4a

Taok2--

  *** xMekk1[tAb] copptby[WB] xTaok2[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** source: 10969079-Fig-1

