======================
Direct Phosphorylation
======================

  *** Foxo3[Ab]IP phos(S253)[phosAb] is increased by pAkt1{act}
    *** cells: none
    *** IPfrom: PC12 in BMS "treated with Wortmannin"
    *** source: 11953455-Fig-7
  *** Foxo3[Ab]IP phos(T32)[phosAb] is increased by pAkt1{act}
    *** cells: none
    *** IPfrom: PC12 in BMS "treated with Wortmannin"
    *** source: 11953455-Fig-7

============================
Semi-direct Phosphorylation
============================

phos(T32)

  *** rFoxo3[phosAb] phos(T32)[phosAb] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(mnr)"CA"> in BMS
    *** source: 11154281-Fig-1c
  *** rFoxo3[phosAb] phos(T32)[phosAb] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293T<xAkt1(mnr)"CA"> in BMS
    *** source: 10102273-Fig-1b
  *** rFoxo3[32P] phos[32P-ATP] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293T<xAkt1(mnr)"CA"> in BMS
    *** partially inhibited by: rFoxo3(T32A) [substitution]
    *** source: 10102273-Fig-1a
  *** rFoxo3[32P] phos[32P-ATP] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(mnr)"CA"> in BMS
    *** unaffected by: rFoxo3(T32A) [substitution]
    *** inhibited by: rFoxo3(T32A/T253A) [substitution]
    *** unaffected by: rFoxo3(T32A/S315A) [substitution]
    *** inhibited by: rFoxo3(T32A/T253A/S315A) [substitution]
    *** source: 11154281-Fig-1b

phos(S253)

  *** rFoxo3[phosAb] phos(S253)[phosAb] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(mnr)"CA"> in BMS
    *** source: 11154281-Fig-1c
  *** rFoxo3[phosAb] phos(S253)[phosAb] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293T<xAkt1(mnr)"CA"> in BMS
    *** source: 10102273-Fig-1b
  *** rFoxo3[32P] phos[32P-ATP] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293T<xAkt1(mnr)"CA"> in BMS
    *** inhibited by: rFoxo3(S253A) [substitution]
    *** source: 10102273-Fig-1a
  *** rFoxo3[32P] phos[32P-ATP] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(mnr)"CA"> in BMS
    *** inhibited by: rFoxo3(T253A) [substitution]
    *** inhibited by: rFoxo3(T32A/T253A) [substitution]
    *** inhibited by: rFoxo3(T253A/S315A) [substitution]
    *** inhibited by: rFoxo3(T32A/T253A/S315A) [substitution]
    *** source: 11154281-Fig-1b

phos(S315)

  *** rFoxo3[phosAb] phos(S315)[phosAb] is unchanged by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(mnr)"CA"> in BMS
    *** source: 11154281-Fig-1c
  *** rFoxo3[32P] phos[32P-ATP] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(mnr)"CA"> in BMS
    *** unaffected by: rFoxo3(S315A) [substitution]
    *** unaffected by: rFoxo3(T32A/S315A) [substitution]
    *** inhibited by: rFoxo3(T253A/S315A) [substitution]
    *** inhibited by: rFoxo3(T32A/T253A/S315A) [substitution]
    *** source: 11154281-Fig-1b
  *** rFoxo3[32P] phos[32P-ATP] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293T<xAkt1(mnr)"CA"> in BMS
    *** unaffected by: rFoxo3(S315A) [substitution]
    *** source: 10102273-Fig-1a
  *** xFoxo3[phosAb] phos[WBMS] is increased itpo xAkt1(mnr)"CA"
    *** cells: CCL39<xFoxo3> in BMLS
    *** inhibited by: xFoxo3(S315A) [substitution]
    *** source: 10102273-Fig-2b

total phos

  *** rFoxo3[32P] phos[32P-ATP] is increased by xAkt1(mnr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293T<xAkt1(mnr)"CA"> in BMS
    *** inhibited by: xAkt1(mnr)"KD" [substitution]
    *** source: 10102273-Fig-1a
  *** xFoxo3[phosAb] phos[WBMS] is increased itpo xAkt1(mnr)"CA"
    *** cells: CCL39<xFoxo3> in BMLS
    *** unaffected by: xFoxo3(T32A/S253A) [substitution]
    *** source: 10102273-Fig-2b

=====================
Basal Phosphorylation
=====================

phos(S253)

  *** Foxo3[phosAb] phos(T32)[phosAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xAkt1(K197M)"KD" [addition]
    *** source: 11154281-Fig-4

phos(S253)

  *** Foxo3[phosAb] phos(S253)[phosAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xAkt1(K197M)"KD" [addition]
    *** source: 11154281-Fig-4

phos(S315)

  *** Foxo3[phosAb] phos(S315)[phosAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xAkt1(K197M)"KD" [addition]
    *** source: 11154281-Fig-4

======================
Akt1-CA Overexpression
======================

phos(T32)

  *** xFoxo3[phosAb] phos(T32)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: CCL39<xFoxo3> in BMS
    *** source: 11154281-Fig-2a
  *** xFoxo3[phosAb] phos(T32)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: HEK293T<xFoxo3> in BMS
    *** source: 11154281-Fig-2a
  *** xFoxo3[phosAb] phos(T32)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: CCL39<xFoxo3> in BMLS
    *** source: 10102273-Fig-2b
  *** xFoxo3[phosAb] phos(T32)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: CCL39<xFoxo3> in BMS
    *** source: 11154281-Fig-3

phos(T32/S253)

  *** Foxo3[phosAb] phos(T32/S253)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: PC12 in BMS
    *** source: 11953455-Fig-8a
  *** Foxo3[phosAb] phos(T32/S253)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: HEK293 in BMS
    *** source: 11953455-Fig-8a

phos(S253)

  *** xFoxo3[phosAb] phos(S253)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: CCL39<xFoxo3> in BMS
    *** source: 11154281-Fig-2a
  *** xFoxo3[phosAb] phos(S253)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: HEK293T<xFoxo3> in BMS
    *** source: 11154281-Fig-2a
  *** xFoxo3[phosAb] phos(S253)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: CCL39<xFoxo3> in BMLS
    *** source: 10102273-Fig-2b
  *** xFoxo3[phosAb] phos(S253)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: CCL39<xFoxo3> in BMS
    *** source: 11154281-Fig-3

phos(S315)

  *** xFoxo3[phosAb] phos(S315)[phosAb] is unchanged itpo xAkt1(mnr)"CA"
    *** cells: CCL39<xFoxo3> in BMS
    *** source: 11154281-Fig-2a
  *** xFoxo3[phosAb] phos(S315)[phosAb] is increased itpo xAkt1(mnr)"CA"
    *** cells: HEK293T<xFoxo3> in BMS
    *** source: 11154281-Fig-2a
