=================
Kinase Activation
=================

Pak1-- ???

  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Pdgf (5 min)
    *** cells: NIH3T3 in BMLS
    *** partially inhibited by: xPak1(83-149)"DN" [addition]
    *** source: 16129686-Fig-6

Pin1--

  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Pdgf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5+ 30 min
    *** reqs: Pin1 [KO]
    *** source: 15664191-Fig-5e

Raf1 features

  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Pdgf (5 min)
    *** cells: NIH3T3 in BMLS
    *** inhibited by: xRaf1(S338A) [substitution]
    *** inhibited by: xRaf1(Y341A) [substitution]
    *** unaffected by: xRaf1(S339A) [substitution]
    *** unaffected by: xRaf1(Y340A) [substitution]
    *** source: 10205168-Fig-4c

no extras

  *** Raf1[Ab]IP IVKA(Erk2,Mek1)[32P-ATP] is increased irt Pdgf (5 min)
    *** cells: rSECs in BMLS "Vegfa deprived"
    *** source: 10327068-Fig-1d
  *** Raf1[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Pdgf (5 min)
    *** cells: NIH3T3 in BMS
    *** source: 10205168-Fig-4a
  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 5+ 30 min
    *** source: 15664191-Fig-1c
  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 5++ 15+ 30 120 240 720 min
    *** source: 15664191-Fig-1a
  *** xRaf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 5++ 30+ min
    *** source: 15664191-Fig-1c
  *** xRaf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 5++ 30+ min
    *** source: 15664191-Fig-4a

Chemicals

  *** xRaf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 5++ 30+ min
    *** enhanced by: U0126 [addition]
    *** source: 15664191-Fig-3a
  *** Raf1[Ab]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMS
    *** times: 0 2+ 5++ 10++ 20+ 60+ min
    *** partially inhibited by: Wortmannin [addition]
    *** partially inhibited by: LY294002 [addition]
    *** source: 11259591-Fig-2b
  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Pdgf (5 min)
    *** cells: PC12<xPdgfRb> in BMLS
    *** bkg inhibited by: Forskolin [addition]
    *** source: 7935374-Fig-2b,2c

===============
Phosphorylation
===============

  *** xRaf1[Ab]IP phos(S29/S43/S289/S301/S642)[HPLC-PAA-ED] is increased irt Pdgf (30 min)
    *** cells: NIH3T3 in BMLS
    *** inhibited by: U0126 [addition]
    *** inhibited by: xRaf1(S29A/S289A/S296A/S301A/S43A/S642A) [substitution]
    *** source: 15664191-Fig-2a
  *** Raf1[Ab]IP phos(S338)[phosAb] is increased irt Pdgf (5 min)
    *** cells: NIH3T3 in BMLS
    *** source: 10205168-Fig-4b
  *** Raf1[Ab]IP phos[WBMS-PPase] is increased irt Pdgf (30 min)
    *** cells: NIH3T3 in BMLS
    *** source: 15664191-Fig-1b
  *** Raf1[Ab]IP phos[WBMS] is increased irt Pdgf (times)
    *** cells: mEFs in BMLS
    *** times: 0 30+ 240 min
    *** comment: decrease at 240 min reqs Pin1 [KO]
    *** source: 15664191-Fig-5d
  *** xRaf1[phosAb] phos(S338)[phosAb] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 5+ 30 min
    *** comment: prolonged by xRaf1(S29A/S289A/S296A/S301A/S43A/S642A) [substitution]
    *** source: 15664191-Fig-4d
  *** xRaf1[phosAb] phos(S338)[phosAb] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMS
    *** times: 0 2+ 5++ 10++ 20++ 60++ min
    *** partially inhibited by: Wortmannin [addition]
    *** partially inhibited by: LY294002 [addition]
    *** source: 11259591-Fig-2a
  *** Raf1[phosAb]IP phos(S642)[phosAb] is increased irt Pdgf (times)
    *** cells: mEFs in BMLS
    *** times: 0 30+ 240 min
    *** comment: decrease at 240 min reqs Pin1 [KO]
    *** source: 15664191-Fig-5d
  *** xRaf1[tAb]IP phos(S259)[phosAb] is decreased irt Pdgf (5 min)
    *** cells: NIH3T3 in BMLS
    *** inhibited by: Okadaic-acid [addition]
    *** source: 12932319-Fig-4c
