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Overexpressed Erk2 is located in the nucleus
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reqs phos(S246/S248) (SPS site)

  *** rErk2[Ab] boundby[WB] Ipo7[Ab]IP is detectable
    *** cells: none
    *** IPfrom: HELA in BMLS
    *** inhibited by: rErk2(S246A/S248A) [substitution]
    *** enhanced by: rErk2(S246E/S248E) [substitution]
    *** source: 18760948-Fig-6b
  *** xErk2[tAb] infraction(nuclear)[WB] is detectable
    *** cells: COS7<xErk2> in BMLS
    *** inhibited by: xErk2(S246A/S248A) [substitution]
    *** enhanced by: xErk2(S246E/S248E) [substitution]
    *** source: 18760948-Fig-3b
  *** xErk2[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: CHO<xErk2> in BMLS
    *** inhibited by: xErk2(S246A/P247A/S248A) [substitution]
    *** inhibited by: xErk2(del(246-278)) [substitution]
    *** source: 18760948-Fig-1b
  *** xErk2[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: COS7<xErk2> in BMLS
    *** inhibited by: xErk2(S246A/P247A/S248A) [substitution]
    *** inhibited by: xErk2(del(246-278)) [substitution]
    *** source: 18760948-Fig-1b
  *** xErk2[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: CHO<xErk2> in BMLS
    *** inhibited by: xErk2(S246A/S248A) [substitution]
    *** unaffected by: xErk2(S246E/S248E) [substitution]
    *** source: 18760948-Fig-3a
  *** xErk2[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: CHO<xErk2> in BMLS
    *** inhibited by: xErk2(S246A/S248A) [substitution]
    *** unaffected by: xErk2(S246E/S248E) [substitution]
    *** source: 18760948-Fig-S2
  *** xErk2[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: CHO<xErk2> in BMLS
    *** inhibited by: xErk2(S246A/S248A) [substitution]
    *** unaffected by: xErk2(S246E/S248E) [substitution]
    *** unaffected by: xErk2(S246A/S248E) [substitution]
    *** unaffected by: xErk2(S246E/S248A) [substitution]
    *** source: 18760948-Fig-S4

does not req phos on TEY site

  *** xErk2[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: CHO<xErk2> in BMLS
    *** unaffected by: xErk2(T185A/E186A/Y187A) [substitution]
    *** unaffected by: xErk2(T185A/E186A/Y187A/S246E/S248E) [substitution]
    *** source: 18760948-Fig-S6

Anchored in cytoplasm by Mek1

  *** xErk2[XFP] locatedin(nucleus)[IHC] is decreased itpo xMek1
    *** cells: CHO<xErk2> in BMLS
    *** source: 18760948-Fig-1c
  *** xErk2[XFP] locatedin(nucleus)[IHC] is decreased itpo xMek1
    *** cells: CHO<xErk2> in BMLS
    *** source: 18760948-Fig-S5

does not req Ipo7 in absence of Egf stim

  *** xErk2[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: HELA<xErk2> in BMLS
    *** does not req: Ipo7 [RNAi]
    *** source: 18760948-Fig-5c
















