=========================================
Direct Phosphorylation on S20 but not S15
=========================================

  *** rTp53[phosAb] phos(S20)[phosAb] is increased by rJnk1{act}
    *** cells: none
    *** source: 11896587-Fig-4b
  *** rTp53[phosAb] phos(S20)[phosAb] is increased by rJnk2{act}
    *** cells: none
    *** source: 11896587-Fig-4b

  *** rTp53[phosAb] phos(S15)[phosAb] is unchanged by rJnk1{act}
    *** cells: none
    *** source: 11896587-Fig-4b
  *** rTp53[phosAb] phos(S15)[phosAb] is unchanged by rJnk2{act}
    *** cells: none
    *** source: 11896587-Fig-4b
  *** rTp53[phosAb] phos(S20)[phosAb] is increased by Jnks[phosAb]IP
    *** cells: none
    *** IPfrom: JB6CL41 in BMLS "treated with UV for 30 min"
    *** source: 11896587-Fig-4a


==========================
SemiDirect Phosphorylation
==========================

  *** rTp53[32P] phos[32P-ATP] is increased by xJnk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293T<xJnk2> in BMS "treated with UV for 60 min"
    *** inhibited by: rTp53(T81A) [substitution]
    *** source: 11283254-Fig-1a

  *** rTp53[phosAb] phos(S20)[phosAb] is increased by Jnks[phosAb]IP
    *** cells: none
    *** IPfrom: mEFs in BMLS "treated with UV for 30 min"
    *** reqs: Jnk1 [KO]
    *** reqs: Jnk2 [KO]
    *** source: 11896587-Fig-5b

=============================================================
Tp53 phos(T81) in the presence of proteins that activate Jnks
=============================================================

Mekk1--

  *** xTp53[phosAb] phos(T81)[phosAb] is increased itpo xMekk1(mnr)"CA"
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** inhibited by: xJnk2(mnr)"DN" [addition]
    *** source: 11283254-Fig-1c

Mkk7--

  *** xTp53[phosAb] phos(T81)[phosAb] is increased itpo xMkk7(mnr)"CA"
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** inhibited by: xJnk2(mnr)"DN" [addition]
    *** source: 11283254-Fig-1c
  *** xTp53[phosAb] phos(T81)[phosAb] is increased itpo xMkk7(mnr)"CA"
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** inhibited by: xTp53(mnr)"del-Jnk-BS" [substitution]
    *** source: 11283254-Fig-1c
  *** xTp53[phosAb] phos(T81)[phosAb] is unchanged itpo xMkk6(mnr)"CA"
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** source: 11283254-Fig-1c

  *** xTp53[Ab] prot-exp[WB] is increased itpo xMkk7(mnr)"CA"
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** comment: xTp54 is phosed on T81
    *** source: 11283254-Fig-5b
  *** xTp53[Ab] prot-exp[WB] is increased itpo xMkk7(mnr)"CA"
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** comment: xTp54 is phosed on T81 but not on S15
    *** source: 11283254-Fig-5a
  *** xTp53[Ab]IP prot-stability[35S-pulse-chase] is increased itpo xMkk7(mnr)"CA"
    *** cells: mEFs<Tp53~null><xTp53> in BMS
    *** inhibited by: xTp53(T81A) [substitution]
    *** source: 11283254-Fig-3


=================
Downstream Events
=================

  *** Tp53-reporter[CAT] is increased itpo xJnk1
    *** cells: MCF7 in BMS
    *** source: 10581258-Fig-1c
  *** Tp53-reporter[CAT] is unchanged itpo xJnk1
    *** cells: H1299<Tp53~null> in BMS
    *** source: 10581258-Fig-2c

  *** Tp53[Ab]IP phos(S20)[phosAb] is detectable
    *** cells: JB6CL41 in BMLS "treated with UV for 4 hr"
    *** inhibited by: xJnk1(mnr)"DN" [addition]
    *** source: 11896587-Fig-2b
