=============
Transcription
=============

  *** Cdkn1a-gene mRNA[Northern] is increased itpo xTp53(A138V)
    *** cells: DP161 in BMS
    *** source: 12654245-Fig-1b
  *** Cdkn1a-gene mRNA[RTPCR] is increased itpo xTp53
    *** cells: H1299<Tp53~null> in BMS
    *** unaffected by: xTp53(S46A) [substitution]
    *** unaffected by: xTp53(del(46-46)) [substitution]
    *** inhibited by: xTp53(S121F) [substitution]
    *** source: 11030628-Fig-8b

  *** Cdkn1a-gene promo-reporter[CAT] is increased itpo xTp53
    *** cells: H1299<Tp53~null> in BMS
    *** source: 10581258-Fig-2d
  *** Cdkn1a-gene promo-reporter[CAT] is increased itpo xTp53
    *** cells: MCF7 in BMS
    *** source: 10581258-Fig-1d
  *** Cdkn1a-gene promo-reporter[Luc] is increased itpo xTp53
    *** cells: SAOS2<Tp53~null> in BMS
    *** unaffected by: xTp53(S46A) [substitution]
    *** source: 11030628-Fig-8c

  *** Cdkn1a-gene mRNA[RTPCR] is detectable
    *** cells: mEFs in BMS "treated with Adriamycin for 6 hr"
    *** inhibited by: kTp53(S18A) [knockin] 
    *** inhibited by: kTp53(S18A/S23A) [knockin] 
    *** source: 16757976-Fig-2a
  *** Cdkn1a-gene mRNA[RTPCR] is detectable
    *** cells: mEFs in BMS "treated with Adriamycin for 12 hr"
    *** inhibited by: kTp53(S18A) [knockin] 
    *** inhibited by: kTp53(S18A/S23A) [knockin] 
    *** source: 16757976-Fig-2a
  *** Cdkn1a-gene mRNA[RTPCR] is detectable
    *** cells: mThyCs in BMS "treated with IR for 18 hr"
    *** unaffected by: kTp53(S18A) [knockin] 
    *** inhibited by: kTp53(S18A/S23A) [knockin] 
    *** reqs: Tp53 [KO]
    *** source: 16757976-Fig-4b

===========
Translation
===========

requires Tp53 R175

  *** Cdkn1a[Ab] prot-exp[WB] is increased itpo xTp53
    *** cells: H1299<Tp53~null> in BMS
    *** inhibited by: xTp53(R175H) [substitution]
    *** source: 15103385-Fig-5d

requires Tp53 S121

  *** Cdkn1a[Ab] prot-exp[WB] is increased itpo xTp53
    *** cells: H1299<Tp53~null> in BMS
    *** unaffected by: xTp53(S46A) [substitution]
    *** unaffected by: xTp53(del(1-46)) [substitution]
    *** inhibited by: xTp53(S121F) [substitution]
    *** source: 11030628-Fig-8a

requires Tp53 K164

  *** Cdkn1a[Ab] prot-exp[WB] is increased itpo xTp53
    *** cells: H1299<Tp53~null> in BMS
    *** inhibited by: xTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-3c
  *** Cdkn1a[Ab] prot-exp[WB] is increased itpo xTp53
    *** cells: H1299<Tp53~null> in BMS
    *** inhibited by: xTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-7a
  *** Cdkn1a[Ab] prot-exp[WB] is increased itpo xTp53
    *** cells: H1299<Tp53~null> in BMS
    *** partially inhibited by: xTp53(K120R/K164R) [substitution]
    *** partially inhibited by: xTp53(K120R/K370R/K372R/K373R/K381R/K382R/K386R)"K120R/6KR" [substitution]
    *** inhibited by: xTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-2b
  *** Cdkn1a[Ab] prot-exp[WB] is increased itpo xTp53
    *** cells: H1299<Tp53~null> in BMS
    *** partially inhibited by: xTp53(K164R) [substitution]
    *** unaffected by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R)"6KR" [substitution]
    *** unaffected by: xTp53(K164R/K370R/K372R/K373R/K381R/K382R/K386R)"7KR" [substitution]
    *** inhibited by: xTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-2b
  *** Cdkn1a[Ab] prot-exp[WB] is increased itpo xTp53
    *** cells: H1299<Tp53~null> in BMS
    *** unaffected by: xTp53(K120R) [substitution]
    *** source: 18485870-Fig-S2b

  *** Cdkn1a[Ab] prot-exp[WB] is increased itpo xTp53
    *** cells: U2OS in BMS
    *** enhanced by: xHipk2 [addition]
    *** source: 11740489-Fig-7e
  *** Cdkn1a[Ab] prot-exp[WB] is unchanged itao Tp53 [RNAi]
    *** cells: MCF7 in BMS
    *** source: 12963717-Fig-3a

================
Promoter Binding
================

does not require Tp53 phos(S33/S46/T81/S127/T150/S315)

  *** xTp53[Ab] boundto(Cdkn1a-gene)[ChIP] is detectable
    *** cells: SAOS2<Tp53~null><xTp53> in BMS "treated with Etoposide for 12 hr"
    *** inhibited by: xTp53(S33A/S46A/T81A/S315A) [substitution]
    *** source: 17906639-Fig-3d
  *** xTp53[Ab] boundto(Cdkn1a-gene)[ChIP] is detectable
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(S33A/S46A/T81A/S315A) [substitution]
    *** source: 17906639-Fig-3c

requires Tp53 acetyl sites

  *** xTp53[Ab] boundto(Cdkn1a-gene)[ChIP] is detectable
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** partially inhibited by: xTp53(K319R/K320R/K321R/K370R/K372R/K373R/K381R/K382R/K386R)"9KR" [substitution] 
    *** source: 17906639-Fig-5a

  *** xTp53[Ab] boundto(Cdkn1a-gene)[ChIP] is detectable
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-4a
  *** xTp53[Ab] boundto(Cdkn1a-gene)[ChIP] is detectable
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-4c
  *** xTp53[Ab] boundto(Cdkn1a-gene)[ChIP] is detectable
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xMdm2 [addition]
    *** unaffected by: xCbp + xTip60 + xMdm2 [addition]
    *** source: 18485870-Fig-4a
  *** xTp53[Ab] boundto(Cdkn1a-gene)[ChIP] is detectable
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xMdm2 [addition]
    *** unaffected by: xCbp + xTip60 + xMdm2 [addition]
    *** source: 18485870-Fig-4c

requires Pin1 in untreated cells only

  *** xTp53[Ab] boundto(Cdkn1a-gene)[ChIP] is detectable
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** partially reqs: Pin1 [RNAi]
    *** source: 17906639-Fig-5b

  *** Tp53[Ab] boundto(Cdkn1a-gene)[ChIP] is detectable
    *** cells: U2OS in BMS "treated with Etoposide for 12 hr"
    *** does not req: Pin1 [RNAi]
    *** source: 17906639-Fig-S5b

============================================
Cofactors, Inhibitors, and Upstream Proteins
============================================

E4f1--

  *** Tp53[Ab] boundto(Cdkn1a-gene)[ChIP] is increased itpo xE4f1
    *** cells: U2OS in BMS
    *** source: 17110336-Fig-6e
  *** Cdkn1a-gene mRNA[RTPCR] is increased itpo xE4f1
    *** cells: U2OS in BMS
    *** reqs: Tp53 [RNAi]
    *** source: 17110336-Fig-5a
  *** Cdkn1a-gene mRNA[RTPCR] is increased itpo xE4f1
    *** cells: U2OS in BMS
    *** unaffected by: xE4f1(del(41-85)) [substitution]
    *** source: 17110336-Fig-5a






















