==============
Direct Binding
==============

  *** rApc(1211-2075)[GST] boundby[WB] rAxin1[MalBP] is detectable
    *** cells: none
    *** unaffected by: rDvl1 [addition]
    *** source: 10330181-Fig-5d
  *** rApc(1211-2075)[Ab] boundby[WB] rAxin1[MalBP] is detectable
    *** cells: none
    *** comment: unaffected by rAxin phos by Gsk3b
    *** comment: unaffected by rApc phos by Gsk3b
    *** source: 10698523-Fig-2e
  *** rApc(1211-2075)[Ab] boundby[WB] rAxin1[MalBP] is detectable
    *** cells: none
    *** unaffected by: rGsk3b [addition]
    *** source: 10698523-Fig-2d

===============
Coprecipitation
===============

does not req Btrc--

  *** Apc[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293T<xBtrc><xAxin1> in BMS
    *** does not req: xBtrc [omission]
    *** source: 10228155-Fig-3b

does not req Csnk1e--

  *** xAxin1[tAb] copptby[WB] xApc[tAb] is detectable
    *** cells: COS<xAxin1><xApc> in BMS
    *** unaffected by: xCsnk1e [addition]
    *** unaffected by: xCsnk1e(mnr)"KD" [addition]
    *** source: 11425858-Fig-7

does not req Dvl1--

  *** xAxin1[tAb] copptby[WB] xApc[tAb] is detectable
    *** cells: COS<xAxin1><xApc><xCsnk1e> in BMS
    *** unaffected by: xDvl1 [addition]
    *** source: 11425858-Fig-7

does not req Fbxw2--

  *** Apc[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293T<xFbxw2><xAxin1> in BMS
    *** does not req: xFbxw2 [omission]
    *** source: 10228155-Fig-3b

inhibited by Mekk1--  ???  Competition?

  *** xApc[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293T<xAxin1><xApc><xCtnnb1(mnr)"delN"> in BMS
    *** inhibited by: xMekk1(mnr)"C" [addition]
    *** source: 11408485-Fig-5c
  *** xAxin1[Ab] copptby[WB] xApc[Ab] is detectable
    *** cells: HEK293T<xAxin1><xApc><xCtnnb1(mnr)"delN"> in BMS
    *** partially inhibited by: xMekk1(mnr)"C" [addition]
    *** source: 11408485-Fig-5c

required Axin1 features--

  *** Apc[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293<xAxin1> in BMS
    *** inhibited by: xAxin1(284-500) [substitution]
    *** inhibited by: xAxin1(284-712) [substitution]
    *** inhibited by: xAxin1(475-676) [substitution]
    *** inhibited by: xAxin1(712-827) [substitution]
    *** source: 11487578-Fig-6a

No extras--

  *** Apc[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293<xAxin1><xMyc> in BMS
    *** source: 19131971-Fig-1d
  *** xApc[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293T<xAxin1><xApc> in BMS
    *** source: 11408485-Fig-5b
  *** xAxin1[Ab] copptby[WB] xApc[Ab] is detectable
    *** cells: HEK293T<xAxin1><xApc> in BMS
    *** source: 11408485-Fig-5b

=======================================================
Coprecipitation with other members of the Axin1 complex
=======================================================

Btrc--

  *** Apc[Ab] copptby[WB] xBtrc[tAb] is detectable
    *** cells: HEK293 in BMS "treated with ALLN for 4 hr"
    *** partially inhibited by: xBtrc(241-569)"WD1-7" [substitution]
    *** inhibited by: xBtrc(291-569)"WD2-7" [substitution]
    *** inhibited by: xBtrc(1-247)"Fbox" [substitution]
    *** inhibited by: xBtrc(1-306)"FWD1" [substitution]
    *** source: 10074433-Fig-2c
  *** Apc[Ab] copptby[WB] xBtrc[tAb] is detectable
    *** cells: HEK293T<xBtrc><xAxin1> in BMS
    *** reqs: xAxin1 [omission]
    *** source: 10228155-Fig-3b

Ctnnb1--

  *** xApc[Ab] copptby[WB] xCtnnb1(mnr)"delN"[tAb] is detectable
    *** cells: HEK293T<xAxin1><xApc><xCtnnb1(mnr)"delN"> in BMS
    *** unaffected by: xMekk1(mnr)"C" [addition]
    *** source: 11408485-Fig-5c
  *** xCtnnb1(mnr)"delN"[tAb] copptby[WB] xApc[Ab] is detectable
    *** cells: HEK293T<xAxin1><xApc><xCtnnb1(mnr)"delN"> in BMS
    *** unaffected by: xMekk1(mnr)"C" [addition]
    *** source: 11408485-Fig-5c
  *** xCtnnb1[Ab] copptby[WB] xApc(1711-1742)"Apc#3"[Ab] is detectable
    *** cells: NEURO2A<xAxin2><xCtnnb1><xApc(1711-1742)> in BMS
    *** reqs: xAxin2 [omission]
    *** source: 9554852-Fig-2b
  *** Apc[Ab] copptby[WB] Ctnnb1[Ab] is detectable
    *** cells: SW480 in BMS
    *** unaffected by: xGsk3b [addition]
    *** unaffected by: xGsk3b(K85M/K86I)"KD" [addition]
    *** source: 8638126-Fig-1

Gsk3b--

  *** Apc[Ab] copptby[WB] xGsk3b[tAb] is detectable
    *** cells: SW480<xGsk3b> in BMS
    *** source: 8638126-Fig-1

