==================================================
Proteins required for Ripk1 ubiquitination irt Tnf
==================================================

not Rnf11--

  *** Ripk1[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: THP1 in BMS
    *** times: 0 30+ 120+ min
    *** repressed by: Rnf11 [RNAi]
    *** source: 19131965-Fig-4a

not Tnfaip3--

  *** Ripk1[Ab] upshift[WBMS] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 5++ 15+ min
    *** does not req: Tnfaip3 [RNAi]
    *** source: 19910467-Fig-S1e

Traf2--

  *** Ripk1[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 10+ 20+ 40+ min
    *** reqs: Traf2 [KO]
    *** source: 15175328-Fig-6b
  *** Ripk1[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 10+ 20+ min
    *** reqs: Traf2 and/or Traf5 [DKO]
    *** source: 15175328-Fig-6c

Tradd--

  *** Ripk1[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 2 5+ min
    *** reqs: Tradd [KO]
    *** source: 18641654-Fig-1e

Ube2ds--

  *** Ripk1[Ab] upshift[WBMS] is increased irt Tnf (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 10++ min
    *** reqs: Ube2ds [RNAi]
    *** source: 19854138-Fig-S4a

not Usp21--  required for decrease at 30 min

  *** Ripk1[Ab] upshift[WBMS] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 5++ 15+ min
    *** bkg repressed by: Usp21 [RNAi]
    *** source: 19910467-Fig-S1e
  *** Ripk1[Ab]IP upshift[WBMS] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 5++ 15++ 30+ min
    *** comment: decrease at 30 min reqs Usp21 [RNAi]
    *** source: 19910467-Fig-4a

===============================================
Ripk1 sites required for ubiquitination irt Tnf
===============================================

  *** Ripk1[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: mEFs<Ripk1~null><xRipk1> in BMS
    *** times: 0 30+ 60+ min
    *** unaffected by: xRipk1(D138N) [substitution]
    *** source: 15175328-Fig-4
  *** xRipk1[tAb]IP ubiq[UbAb] is increased irt Tnf (4 min)
    *** cells: JURKAT<Pten~null><Ripk1~null><xRipk1> in BMS
    *** reqs: xRipk1 [omission]
    *** inhibited by: xRipk1(K377R) [substitution]
    *** source: 16543241-Fig-5a

==================
Ubiq is K63 linked
==================

  *** Ripk1[Ab]IP ubiq[tUbAb] is increased irt Tnf (2 min)
    *** cells: JURKAT<Pten~null><xUbiq> in BMS
    *** inhibited by: xUbiq(K63R) [substitution]
    *** inhibited by: xUbiq(K6R/K11R/K27R/K29R/K33R/K63R) [substitution]
    *** unaffected by: xUbiq(K6R/K11R/K27R/K29R/K33R/K48R) [substitution]
    *** unaffected by: xUbiq(K48R) [substitution]
    *** source: 16603398-Fig-1b

==========
So.....??
==========

  *** Ripk1[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: HELAS3 in BMS "Nemo-precipitates"
    *** times: 0 1+ 5++ 15+ 30+ min
    *** comment: Nemo-IPs were heated in 1% SDS to remove noncovalently attached proteins before Ripk1-IP
    *** source: 16547522-Fig-4e
  *** Ripk1[Ab] copptby[WB] Nemo[Ab] is increased irt Tnf (3 min)
    *** cells: HELAS3 in BMS
    *** comment: coppt Ripk is ubiq [WBMS]
    *** reqs: Ubc13 [RNAi]
    *** source: 16547522-Fig-4f
  *** Ripk1[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 10++ 20+ 40 90 120 240 min
    *** comment: decrease after 20 min requires Tax1bp1 [KO]
    *** source: 20185725-Fig-S2d

=========
no extras
=========

  *** Ripk1[Ab]IP ubiq[UbAb] is increased irt Tnf (10 min)
    *** cells: RAW2647 in BMS
    *** source: 16115877-Fig-8c
  *** Ripk1[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 30+ 60+ min
    *** source: 15175328-Fig-1c
  *** Ripk1[Ab] upshift[WBMS] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 15++ 30+ 60 90 120 min
    *** source: 19910467-Fig-S1d
  *** Ripk1[Ab]IP upshift[WBMS] is increased irt Tnf (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 5++ 15++ 30+ min
    *** source: 19910467-Fig-2e


  *** Ubiq[K63UbAb] copptby[WB] Ripk1[Ab] is increased irt Tnf (times)
    *** cells: mBMMs in BMS
    *** times: 0 15+ 30+ 120 min
    *** comment: decrease after 30 min reqs Ikk1 [RNAi]
    *** source: 21765415-Fig-6e
  *** Ubiq[K63UbAb] copptby[WB] Ripk1[Ab] is increased irt Tnf (times)
    *** cells: mEFs<Tax1bp1~null><xTax1bp1> in BMS
    *** times: 0 15++ 30++ 60+ 120 min
    *** comment: decrease after 30 min reqs xTax1bp1 [omission]
    *** comment: decrease after 30 min inhibited by xTax1bp1(S593A/S666A) [substitution]
    *** source: 21765415-Fig-3a


