Requires Atm phos site on H2ax but not Atm ???

=================
Relocation to DDF
=================

not Atm--

  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: AT22IJET<Atm~fmut><xAtm> in BMS "treated with IR for 20 min"
    *** does not req: xAtm [omission]
    *** source: 12607003-Fig-3b
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS "treated with MicroLaser for 10 min"
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 20026654-Fig-5b
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS "treated with MicroLaser for 60 min"
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 20026654-Fig-5b
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: GM07166<Nbs1~fmut><xNbs1><xAtm> in BMS "treated with MicroLaser for 10 min"
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 20026654-Fig-6b
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: GM07166<Nbs1~fmut><xNbs1><xAtm> in BMS "treated with MicroLaser for 60 min"
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 20026654-Fig-6b

not Atr--

  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 60 min"
    *** does not req: Atr [RNAi]
    *** source: 12607003-Fig-3b

not Brca1--

  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: hFibros in BMS "treated with IR for 1 hr"
    *** does not req: Brca1 [RNAi]
    *** source: 19001859-Fig-6a

H2ax--

  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (30 min)
    *** cells: mEFs in BMS
    *** reqs: H2ax [KO]
    *** source: 12607005-Fig-4a
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (10 min)
    *** cells: U2OS in BMS
    *** reqs: H2ax [RNAi]
    *** source: 15201865-Fig-6a
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** reqs: H2ax [RNAi]
    *** source: 16618811-Fig-S3b
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (tnr)
    *** cells: MCF7 in BMS
    *** reqs: H2ax [RNAi]
    *** source: 12551934-Fig-2c
  ------------ reqs phos on S140 and Y143 -------------
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<H2ax~null><xH2ax> in BMS "treated with IR for 1 hr"
    *** reqs: xH2ax [omission]
    *** inhibited by: xH2ax(S137A/S140A) [substitution]
    *** inhibited by: xH2ax(Y143A) [substitution]
    *** source: 16377563-Fig-4d
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<H2ax~null><xH2ax> in BMS "treated with IR for 30 min"
    *** reqs: xH2ax [omission]
    *** inhibited by: xH2ax(Y143A) [substitution]
    *** source: 16377563-Fig-5b

not Nbs1--

  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: NBS1LBI<Nbs1~fmut> in BMS "treated with IR for 30 min"
    *** source: 12607003-Fig-3c
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: NBST<Nbs1~fmut><xNbs1> in BMS "treated with IR for tnr"
    *** unaffected by: xNbs1(del(24-108))"D1" [substitution]
    *** unaffected by: xNbs1(del(109-196))"D2" [substitution]
    *** unaffected by: xNbs1(del(361-460))"D3" [substitution]
    *** unaffected by: xNbs1(del(261-460))"D4" [substitution]
    *** unaffected by: xNbs1(del(219-460))"D5" [substitution]
    *** source: 18678890-Fig-5a
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: GM07166<Nbs1~fmut><xNbs1><xAtm> in BMS "treated with MicroLaser for 10 min"
    *** unaffected by: xNbs1(mnr)"delFHA" [substitution]
    *** source: 20026654-Fig-6b
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: GM07166<Nbs1~fmut><xNbs1><xAtm> in BMS "treated with MicroLaser for 60 min"
    *** unaffected by: xNbs1(mnr)"delFHA" [substitution]
    *** source: 20026654-Fig-6b

not Prkdc--

  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: M059J<Prkdc~fmut> in BMS "treated with IR for 20 min"
    *** source: 12607003-Fig-3b

not Rnf8--

  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** does not req: Rnf8 [RNAi]
    *** source: 18001825-Fig-4a
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** does not req: Rnf8 [RNAi]
    *** source: 18001825-Fig-S4a
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: U2OS in BMS "treated with MicroLaser for 1 hr"
    *** does not req: Rnf8 [RNAi]
    *** source: 18001824-Fig-4a
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS "treated with MicroLaser for 10 min"
    *** does not req: Rnf8 [RNAi]
    *** source: 20026654-Fig-6c
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS "treated with MicroLaser for 60 min"
    *** does not req: Rnf8 [RNAi]
    *** source: 20026654-Fig-6c
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Rnf8 [RNAi]
    *** source: 18006705-Fig-2b

not Tp53bp1--

  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: hFibros in BMS "treated with IR for 1 hr"
    *** does not req: Tp53bp1 [RNAi]
    *** source: 19001859-Fig-6a
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS in BMS
    *** times: 0 2+ 4++ 6+++ 8+++ 10+++ 12+++ 14+++ 16+++ min
    *** does not req: Tp53 [RNAi]
    *** source: 16009723-Fig-4c
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (tnr)
    *** cells: MCF7 in BMS
    *** does not req: Tp53bp1 [RNAi]
    *** source: 12551934-Fig-2e

not Ubc13--

  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** does not req: Ubc13 [RNAi]
    *** source: 18001825-Fig-S5d

Mdc1 features

  ----------- does not req Atm phos sites (T699/T719/T752/T765) ----
  *** xMdc1[tAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<Mdc1~null><xMdc1> in BMS "treated with IR for 6 hr"
    *** unaffected by: xMdc1(del(698-768)) [substitution]
    *** source: 18001825-Fig-4b
  *** xMdc1[tAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<Mdc1~null><xMdc1> in BMS "treated with IR for 6 hr"
    *** unaffected by: xMdc1(del(698-768)) [substitution]
    *** source: 18001825-Fig-S3b
  *** xMdc1[tAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS<xRnf8><Mdc1~kd><xMdc1> in BMS "treated with IR for 30 min"
    *** unaffected by: xMdc1(T699A/T719A/T752A) [substitution]
    *** source: 18001824-Fig-2g
  *** xMdc1[tAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<Mdc1~null><xMdc1> in BMS "treated with IR for tnr"
    *** unaffected by: xMdc1(del(200-420))"dSDTD" [substitution]
    *** unaffected by: xMdc1(del(698-768))"dTQXF" [substitution]
    *** source: 18678890-Fig-4b
  *** xMdc1[XFP] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS "treated with IR for tnr"
    *** unaffected by: xMdc1(T699A/T719A/T752A/T765A) [substitution]
    *** source: 18006705-Fig-2b
  *** xMdc1[XFP] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS "treated with IR for tnr"
    *** unaffected by: xMdc1(T699A/T719A/T752A/T765A) [substitution]
    *** source: 18006705-Fig-S11a
  *** xMdc1[XFP] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS "treated with IR for tnr"
    *** unaffected by: xMdc1(T699A/T719A/T752A/T765A) [substitution]
    *** source: 18006705-Fig-S11b
  ----------- reqs Mdc1 FHA domain (56-124) ----------------------
  *** xMdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<Mdc1~null><xMdc1> in BMS "treated with IR for tnr"
    *** unaffected by: xMdc1(del(1894-2089))"delBRCT" [substitution]
    *** inhibited by: xMdc1(del(56-124))"delFHA" [substitution]
    *** source: 16427009-Fig-5f
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 30 min"
    *** inhibited by: xMdc1(2-220)"FHA" [addition]
    *** source: 12607003-Fig-4d
  ----------- reqs Mdc1 BRCT domain (1893-2092) ----------------------
  *** xMdc1[tAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA<xMdc1> in BMS "treated with IR for tnr"
    *** inhibited by: xMdc1(del(33-154))"D1" [substitution]
    *** unaffected by: xMdc1(del(125-170))"D2" [substitution]
    *** unaffected by: xMdc1(del(200-420))"D3" [substitution]
    *** unaffected by: xMdc1(del(421-624))"D4" [substitution]
    *** unaffected by: xMdc1(del(625-1129))"D5" [substitution]
    *** unaffected by: xMdc1(del(1130-1661))"D6" [substitution]
    *** unaffected by: xMdc1(del(1662-1892))"D7" [substitution]
    *** inhibited by: xMdc1(del(1893-2092))"D8" [substitution]
    *** source: 18678890-Fig-2a
  *** xMdc1[tAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HEK293T<xMdc1> in BMS "treated with IR for 1 hr"
    *** inhibited by: xMdc1(R1988Q) [substitution]
    *** inhibited by: xMdc1(K1936M) [substitution]
    *** source: 16377563-Fig-4c
  ----------- other MDC1 fragments --------------------------------
  *** xMdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (4 hr)
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS
    *** unaffected by: xMdc1(del(196-506)) [substitution] 
    *** source: 18583988-Fig-4c
  *** xMdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (4 hr)
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS
    *** unaffected by: xMdc1(del(196-506)) [substitution] 
    *** source: 18583988-Fig-S4a
  *** xMdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS "treated with MicroLaser for 30 min"
    *** unaffected by: xMdc1(del(196-506)) [substitution] 
    *** source: 18583988-Fig-4e
  ----------- other MDC1 phos sites --------------------------------
  *** xMdc1[tAb] locatedin(DSBs)[IHC] is detectable
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS "treated with MicroLaser for 1 hr"
    *** unaffected by: xMdc1(S299A/T301A/S453A/T455A) [substitution]
    *** unaffected by: xMdc1(S299A/T301A/S329A/T331A/S402A/T404A/S453A/T455A) [substitution]
    *** source: 18411307-Fig-7b


no extras

  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** comment: Mdc1 phos(S329/T331)[phosAb] shows the same pattern
    *** source: 18583988-Fig-2b
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (4 hr)
    *** cells: U2OS in BMS
    *** source: 18583988-Fig-4c
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (4 hr)
    *** cells: U2OS in BMS
    *** source: 18583988-Fig-S4a
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: M059K in BMS "treated with IR for 20 min"
    *** source: 12607003-Fig-3b
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: NotReported in BMS
    *** times: 0 15+ 30+ min
    *** source: 12607005-Fig-2a
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs in BMS "treated with IR for tnr"
    *** source: 18678890-Fig-4a
  *** xMdc1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS<xMdc1> in BMS
    *** times: 0 30+ 60++ 120+++ 240+++ 360+++ 480+++ 600+++ sec
    *** source: 15201865-Fig-1a
  *** xMdc1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (14 min)
    *** cells: U2OS<xMdc1> in BMS
    *** comment: increase was monitored continuously during MicroLaser
    *** comment: increase was similar to xRnf8 xNbs1 in same system
    *** comment: increase was faster than xBrca1 xTp53bp1 xAtr in same system
    *** source: 18001824-Fig-3b
  *** xMdc1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS<xMdc1> in BMS
    *** times: 0 30+ 60++ 120+++ 360++++ sec
    *** source: 18001824-Fig-3a
  *** xMdc1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS<xMdc1> in BMS
    *** source: 16618811-Fig-9a
  *** xMdc1[XFP] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: U2OS<xMdc1> in BMS
    *** comment: subnuclear bodies are larger than those occupied by xTp53bp1 xAtr xRad52
    *** source: 16618811-Fig-9b
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS in BMS
    *** times: 0 1 2+ 5++ 10+++ 20+++ min
    *** source: 16009723-Fig-3c
  *** xMdc1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS<xMdc1> in BMS
    *** times: 0 1 2+ 4++ 6+++ 8+++ 10+++ 14+++ 16+++ min
    *** comment: xMdc1 to DDFs is faster than xTp63bp1
    *** source: 16009723-Fig-3a
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: BJ in BMS
    *** source: 16618811-Fig-S2
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** source: 16618811-Fig-2a
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** source: 16618811-Fig-S1a
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: NotReported in BMS "treated with IR for 15 and 30 min"
    *** source: 12607005-Fig-2b
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (10 min)
    *** cells: U2OS in BMS
    *** source: 15201865-Fig-6a
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (10 min)
    *** cells: U2OS in BMS
    *** source: 15201865-Fig-3a
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: MCF7 in BMS
    *** times: 0 15++++ 30++++ 60+++ 120++ 180+ 360+ min
    *** comment: time measurements are percent cells with foci
    *** source: 12551934-Fig-2a
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: MRC5 in BMS "treated with IR for 30 min"
    *** source: 12607003-Fig-2c
  *** Mdc1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 15+ 480+ min
    *** source: 12607003-Fig-2b
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: U2OS in BMS "treated with MicroLaser for 2 min"
    *** source: 18001824-Fig-S4b

==============================
Location in Chromatin Fraction
==============================

  *** Mdc1[Ab] infraction(chromatin)[WB] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** source: 15201865-Fig-5a

====================
Effect of Cell Cycle
====================

  *** Mdc1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** comment: not limited to cells expressing CyclinA
    *** source: 16618811-Fig-2b
  *** Mdc1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** comment: not limited to cells expressing CyclinB1
    *** source: 16618811-Fig-4c

==================================
Increased coprecipitation with Atm
==================================

  *** Mdc1[Ab] copptby[WB] xAtm[tAb] is increased irt IR (1 hr)
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS
    *** inhibited by: xAtm(S1981A) [substitution]
    *** source: 20026654-Fig-4b
  *** Mdc1[Ab] copptby[WB] xAtm[tAb] is increased irt IR (1 hr)
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS
    *** source: 20026654-Fig-4a
