===========================
Phosphorylation on Tyrosine
===========================

not Fes--

  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (15 min)
    *** cells: M1241 in BMS
    *** unaffected by: xFes(K590E)"KD" [addition]
    *** source: 11160325-Fig-3a
  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (15 min)
    *** cells: M1241<xFes> in BMS
    *** unaffected by: xFes(K590E)"KD" [substitution]
    *** source: 11160325-Fig-3a

IL4R--   Required Features

  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (15 min)
    *** cells: 32D<xIL4R><xIrs1> in BMLS "IL3-deprived"
    *** inhibited by: xIL4R(1-557) [substitution]
    *** inhibited by: xIL4R(1-437) [substitution]
    *** partially inhibited by: xIL4R(Y497F) [substitution]
    *** source: 7760829-Fig-6
  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (30 min)
    *** cells: 32D<xIrs2><xIL4R> in BMS "IL3-deprived"
    *** reqs: xIL4R [omission]
    *** unaffected by: xIL4R(Y713F) [substitution]
    *** source: 11714803-Fig-5c
  *** Stat6[phosAb] phos(Y641)[phosAb] is increased irt IL4 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 15+ 30+ 60+ min
    *** unaffected by: kIL4R(Y500F) [knockin]
    *** source: 14557412-Fig-2h
  *** Stat6[phosAb] phos(Y641)[phosAb] is increased irt IL4 (times)
    *** cells: mSpCs in BMS "blasted with anti-TcRb/IL2"
    *** times: 0 1+ 5++ 15++ 30++ 60++ min
    *** unaffected by: kIL4R(Y500F) [knockin]
    *** source: 14557412-Fig-2d

not Irs2--

  *** Stat6[phosAb] phos(sitenr)[phosAb] is increased irt IL4 (tnr)
    *** cells: mLCs<IgM~neg><IgG~neg> in BMS
    *** does not req: Irs2 [KO]
    *** source: 11739727-Fig-2a

Inhibited by xSocs1--

  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (30 min)
    *** cells: M1241 in BMLS
    *** partially inhibited by: xSocs1 [addition]
    *** source: 10201892-Fig-3a
  *** xStat6[phosAb] phos(Y641)[phosAb] is increased irt IL4 (60 min)
    *** cells: HEK293<xStat6> in BMS
    *** inhibited by: xSocs1 [addition]
    *** source: 15589317-Fig-6
  *** xStat6[Ab]IP Yphos[pYAb] is increased irt IL4 (30 min)
    *** cells: HEK293<xStat6> in BMS
    *** inhibited by: xSocs1 [addition]
    *** source: 10201928-Fig-8

Unaffected/Inhibited by xSocs2--

  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (30 min)
    *** cells: M1241 in BMLS
    *** unaffected by: xSocs2 [addition]
    *** source: 10201892-Fig-3b
  *** xStat6[phosAb] phos(Y641)[phosAb] is increased irt IL4 (60 min)
    *** cells: HEK293<xStat6> in BMS
    *** partially inhibited by: xSocs2 [addition]
    *** source: 15589317-Fig-6

Unaffected/Inhibited by xSocs3--

  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (30 min)
    *** cells: M1241 in BMLS
    *** unaffected by: xSocs3 [addition]
    *** source: 10201892-Fig-3c
  *** xStat6[phosAb] phos(Y641)[phosAb] is increased irt IL4 (60 min)
    *** cells: HEK293<xStat6> in BMS
    *** inhibited by: xSocs3 [addition]
    *** source: 15589317-Fig-6

Unaffected by Tnf--

  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (40 min)
    *** cells: HACAT in BMS
    *** unaffected by: Tnf [cotreatment]
    *** source: 12776189-Fig-2a

Stat6-- Required Features

  *** Stat6[Ab]IP phos(Y641)[phosAb] is increased irt IL4 (30 min)
    *** cells: HACAT in BMLS
    *** unaffected by: xStat6(1-661) [addition]
    *** unaffected by: xStat6(V547A/T548A) [addition]
    *** source: 15199062-Fig-6b
  *** xStat6[tAb]IP Yphos[pYAb] is increased irt IL4 (15 min)
    *** cells: HEK293 in BMS
    *** inhibited by: xStat6(R27A) [substitution]
    *** source: 15153491-Fig-2

Chemicals

  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (15 min)
    *** cells: HELA in BMS
    *** inhibited by: N6-Methyl-2-deoxyadenosine [addition]
    *** source: 15153491-Fig-6a
  *** Stat6[phosAb] phos(sitenr)[phosAb] is increased irt IL4 (5 min)
    *** cells: mSpCs<Cd4~neg><Cd45~neg><Cd8~pos> in BMS "treated with anti-Cd3,anti-Cd28/IL2"
    *** inhibited by: Jak-Inhibitor-I [pretreatment] no-bkg-control
    *** unaffected by: Jak3-Inhibitor-I [pretreatment] no-bkg-control
    *** source: 17200144-Fig-4c
  *** xStat6[tAb]IP Yphos[pYAb] is increased irt IL4 (15 min)
    *** cells: HEK293 in BMS
    *** inhibited by: N6-Methyl-2-deoxyadenosine [addition]
    *** source: 15153491-Fig-5

No extras

  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (15 min)
    *** cells: 32D in BMLS "IL3-deprived"
    *** source: 7760829-Fig-6
  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (15 min)
    *** cells: KIT225 in BMLS "IL2-deprived"
    *** source: 7760829-Fig-4a
  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (15 min)
    *** cells: KIT225 in BMLS "IL2-deprived"
    *** source: 7760829-Fig-4c
  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (times)
    *** cells: hBCs in BMS
    *** times: 0 30+ 120+ 480+ min
    *** source: 12459556-Fig-1a
  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (times)
    *** cells: KIT225 in BMLS "IL2-deprived"
    *** times: 0 3+ 5+ 10+ 15+ 20+ 30+ 60+ min
    *** source: 7760829-Fig-4b
  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (times)
    *** cells: M1241 in BMS
    *** times: 0 30+ 120+ 760+ min
    *** source: 12459556-Fig-1a
  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (times)
    *** cells: RAMOS in BMS
    *** times: 0 30+ 120+ 760+ min
    *** source: 12459556-Fig-1a
  *** Stat6[phosAb] phos(sitenr)[phosAb] is increased irt IL4 (5 min)
    *** cells: mSpCs<Cd4~neg><Cd45~neg><Cd8~pos> in BMS "treated with anti-Cd3,anti-Cd28/IL2"
    *** source: 17200144-Fig-S3
  *** Stat6[phosAb] phos(sitenr)[phosAb] is increased irt IL4 (times)
    *** cells: hPHAblasts in BMS "IL2-PHA-deprived"
    *** times: 0 10+ 30+ min
    *** source: 12435740-Fig-5a
  *** Stat6[phosAb] phos(sitenr)[phosAb] is increased irt IL4 (times)
    *** cells: mSpCs<Cd4~neg><Cd45~neg><Cd8~pos> in BMS "treated with anti-Cd3,anti-Cd28/IL2"
    *** times: 0 5+ 15+ 30+ min
    *** source: 17200144-Fig-4a
  *** Stat6[Ab]IP Yphos[pYAb] is increased irt IL4 (times)
    *** cells: HEPG2 in BMS "cytoplasmic fraction"
    *** times: 0 2+ 4+ 8+ hr
    *** comment: inhibited by 1 hr Ifng pretreatment at 4-8 hr
    *** source: 10201928-Fig-4b

=============
ChIP Activity
=============

  *** Stat6[Ab] boundto(Arg1-gene)[ChIP] is increased irt IL4 (times)
    *** cells: RAW2647 in BMS
    *** times: 0 1+ 2+ 4+ hr
    *** comment: binding is to the enhancer region of the mouse Arg1-gene
    *** source: 15187139-Fig-5a
  *** Stat6[Ab] boundto(CcL17-gene)[ChIP] is increased irt IL4 (1 hr)
    *** cells: hPBTCs in BMS
    *** source: 16810739-Fig-5c
  *** Stat6[Ab] boundto(Socs1-gene)[ChIP] is increased irt IL4 (times)
    *** cells: HACAT in BMLS
    *** times: 0 30+ 120+ 240+ min
    *** source: 15199062-Fig-4

=====================
Relocation to Nucleus
=====================

  *** Stat6[Ab] infraction(nuclear)[WB] is increased irt IL4 (times)
    *** cells: mSpCs<Cd4~neg><Cd45~neg><Cd8~pos> in BMS "treated with anti-Cd3,anti-Cd28/IL2"
    *** times: 0 5+ 15+ 30+ min
    *** source: 17200144-Fig-4a
  *** Stat6[Ab] infraction(nuclear)[WB] is increased irt IL4 (tnr)
    *** cells: M12 in BMS
    *** source: 16537510-Fig-1c
  *** xStat6[tAb] locatedin(nucleus)[IHC] is increased irt IL4 (times)
    *** cells: HEK293 in BMS
    *** times: 0 5+ 60+ min
    *** inhibited by: xStat6(R27A) [substitution]
    *** comment: cells with nuclear Stat6 were not counted and IHC was iffy
    *** source: 15153491-Fig-3
  *** Stat6[Ab]IP infraction(nuclear)[WB] is increased irt IL4 (times)
    *** cells: HEPG2 in BMS
    *** times: 0 2+ 4+ 8+ hr
    *** comment: inhibited by 1 hr Ifng pretreatment at 4-8 hr
    *** source: 10201928-Fig-4a

======================
Oligo-binding Activity
======================

  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (1 hr)
    *** cells: BJAB in BMS
    *** source: 12023955-Fig-4d
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (1 hr)
    *** cells: CTLL2 in BMS "IL2-deprived"
    *** oligo: "N4-Stat6-BS" 5-AACTTCCCAAGAACAGA-3
    *** source: 10843732-Fig-1a
  *** xStat6[Ab] oligo-binding[EMSA] is increased irt IL4 (1 hr)
    *** cells: CTLL2 in BMS "IL2-deprived"
    *** oligo: "N4-Stat6-BS" 5-AACTTCCCAAGAACAGA-3
    *** unaffected by: xStat6(mnr)"del-Cterm" [substitution]
    *** source: 10843732-Fig-1a
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (2 hr)
    *** cells: RAMOS in BMS
    *** oligo: "Lta-Stat" 5-TTCCCAGAA-3
    *** source: 9541585-Fig-3a
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (10 min)
    *** cells: mSpCs in BMS
    *** oligo: "IL4-GAS" 5-GATCAAGACCTTCACAGGAACTTTAATCT-3
    *** source: 9380721-Fig-2b
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (12 hr)
    *** cells: CL01 in BMS "nuclear-extracts"
    *** oligo: GAS 5-GAGCTGTGATTTCCTAGGAAGACAAA-3
    *** enhanced by: Cd40L [cotreatment]
    *** source: 10228008-Fig-7a
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (12 hr)
    *** cells: hTonsillarBCs in BMS "nuclear-extracts"
    *** oligo: GAS 5-GAGCTGTGATTTCCTAGGAAGACAAA-3
    *** enhanced by: Cd40L [cotreatment]
    *** source: 10228008-Fig-7a
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (15 min)
    *** cells: CTFL15 in BMS
    *** oligo: "IL4-promoter" 5-TGATTTCACAGGAAAATT-3
    *** source: 10072514-Fig-1
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (15 min)
    *** cells: DO1110 in BMS
    *** oligo: "IL4-promoter" 5-TGATTTCACAGGAAAATT-3
    *** source: 10072514-Fig-1
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (15 min)
    *** cells: hUVECs in BMS "nuclear-extract"
    *** oligo: "Pselectin-site-1" 5-AGCTTTAACTTCATGGGAAGGGCG-3
    *** source: 10364072-Fig-2a
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (15 min)
    *** cells: hUVECs in BMS "nuclear-extract"
    *** oligo: "Pselectin-site-2" 5-AGCTTCATCTTCCATGGAAGGGGG-3
    *** source: 10364072-Fig-2b
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (15 min)
    *** cells: M1241 in BMS
    *** oligo: "IL4-promoter" 5-TGATTTCACAGGAAAATT-3
    *** source: 10072514-Fig-1
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (15 min)
    *** cells: mBMMastCs in BMS
    *** oligo: "IL4-promoter" 5-TGATTTCACAGGAAAATT-3
    *** source: 10072514-Fig-1
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (20 min)
    *** cells: HT2 in BMS
    *** oligo: "IL4R-GAS" 5-AGCTTCTTCATCTGAAAAGGG-3
    *** source: 8810328-Fig-2c
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (20 min)
    *** cells: HT2 in BMS
    *** oligo: "Irf1-GAS" 5-GCCTGATTTCCCCGAAATGACGG-3
    *** source: 8810328-Fig-2b
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (20 min)
    *** cells: HT29CL16E in BMS
    *** source: 15004182-Fig-6
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (30 min)
    *** cells: BL2 in BMS "nuclear-extracts"
    *** source: 14978013-Fig-4
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (30 min)
    *** cells: HACAT in BMLS "nuclear-extracts"
    *** oligo: "Socs1-dSBE" 5-CCGGCCCCGCCCAGTTTCCGAGGAACTGGGCCGGGGTGGA-3
    *** source: 15199062-Fig-8
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (30 min)
    *** cells: HACAT in BMLS "nuclear-extracts"
    *** oligo: "Socs1-dSBE" 5-CCGGCCCCGCCCAGTTTCCGAGGAACTGGGCCGGGGTGGA-3
    *** source: 15199062-Fig-9a
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (30 min)
    *** cells: hFibros in BMS
    *** source: 11544308-Fig-5
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (30 min)
    *** cells: hFibros in BMS
    *** source: 11544308-Fig-6
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (30 min)
    *** cells: hPBTCs in BMS "nuclear-extracts"
    *** source: 16810739-Fig-5a
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (30 min)
    *** cells: hPBTCs in BMS "nuclear-extracts"
    *** source: 16810739-Fig-5b
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (30 min)
    *** cells: KIT225 in BMLS "IL2-deprived"
    *** oligo: GAS 5-GATCAAGACCTTTCCCAAGAAATCTATC-3
    *** source: 7760829-Fig-5
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (30 min)
    *** cells: mTCs in BMS "treated with anti-Cd3 + anti-Cd28 for 16 hr"
    *** unaffected by: xIkba(mnr)"del-Nterm" [addition]
    *** source: 11160275-Fig-4a
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (30 min)
    *** cells: mThyCs in BMS "treated with PMA + Ionomycin for 20 hr"
    *** partially inhibited by: xIkba(mnr)"del-Nterm" [addition]
    *** source: 11160275-Fig-4b
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (times)
    *** cells: 32D<xIrs2><xIL4R> in BMS "IL3-deprived"
    *** times: 0 2++ 6++ 12+ 24+ 48+ 96 hr
    *** unaffected by: xIL4R(Y713F) [substitution]
    *** source: 11714803-Fig-5d
  *** xStat6[Ab] oligo-binding[EMSA] is increased irt IL4 (times)
    *** cells: BEAS2B in BMS
    *** times: 0 10+ 30+ min
    *** oligo: "Alox15-Stat6-BS" 5-CGGGTAAGACTTTCCTGAGAAACCGGAGGTGAA-3
    *** source: 10657944-Fig-8
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (times)
    *** cells: HT29M3 in BMS
    *** oligo: "Pigr-Stat6-BS" 5-TTTATTCTTCCAAAGAACTGCAGA-3
    *** times: 0 10++ 30++ 120++ 360++ 720+ 1440+ min
    *** source: 11034397-Fig-4
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (tnr)
    *** cells: WIL2NS in BMS
    *** oligo: "Cd23b-GAS" 5-GATCAGGGTGAATTTCTAAGAAAGGGACTGGTGT-3
    *** source: 9110977-Fig-5a
  *** Stat6[Ab] snaggedby[WB] oligo[biotin] is increased irt IL4 (30 min)
    *** cells: HACAT in BMLS
    *** oligo: "Socs1-dSBE" 5-CCGGCCCCGCCCAGTTTCCGAGGAACTGGGCCGGGGTGGA-3
    *** unaffected by: xEts1 [addition]
    *** source: 15199062-Fig-9c
  *** xStat6[RE] oligo-binding[EMSA] is increased irt IL4 (15 min)
    *** cells: HEK293 in BMS
    *** inhibited by: xStat6(R27A) [substitution]
    *** source: 15153491-Fig-2
  *** xStat6[RE] oligo-binding[EMSA] is increased irt IL4 (15 min)
    *** cells: HELA in BMS "nuclear-extract"
    *** inhibited by: N6-Methyl-2-deoxyadenosine [addition]
    *** source: 15153491-Fig-6a
  *** Stat6[Ab] oligo-binding[EMSA] is increased irt IL4 (1 hr)
    *** cells: hTh1 in BMS
    *** enhanced by: IL12 [cotreatment]
    *** source: 9531298-Fig-4a



============
Other Stuff
============

  *** Stat6[Ab] copptby[WB] Irf4[Ab] is detectable-but unchanged irt IL4 (24 hr) 
    *** cells: RAMOS in BMS
    *** source: 10601358-Fig-3b
  *** Stat6[Ab] snaggedby[WB] rIrf4[GST] is detectable-but unchanged irt IL4 (24 hr)
    *** cells: RAMOS in BMS
    *** source: 10601358-Fig-3a

  *** Stat6[Ab] oligo-binding[EMSA] is detectable-but unchanged irt IL4 (1 hr)
    *** cells: hTh2 in BMS
    *** unaffected by: IL12 [cotreatment]
    *** source: 9531298-Fig-4a

  *** xStat6[tAb] copptby[WB] xEts1[tAb] is increased irt IL4 (30 min)
    *** cells: HACAT in BMLS
    *** source: 15199062-Fig-9d
  *** xStat6[tAb] methyl[RMeAb-ppt] is detectable-but unchanged irt IL4 (15 min)
    *** cells: HEK293 in BMS
    *** source: 15153491-Fig-1b
  *** Stat6RE-reporter[Luc] is increased irt IL4 (40 min)
    *** cells: HACAT in BMS
    *** unaffected by: Tnf [cotreatment]
    *** source: 12776189-Fig-2b
