===============
Kinase Activity
===============

  *** Eef2k[Ab]IP IVKA(Eef2)[32P-ATP] is decreased irt Ins (30 min)
    *** cells: KB in BMLS
    *** reversed by: Rapamycin [pretreatment]
    *** source: 15024086-Fig-6c

======================
Phosphorylation on S78
======================

  *** Eef2k[Ab]IP phos(S78)[phosAb] is increased irt Ins (30 min)
    *** cells: CHOK1 in BMLS
    *** comment: unaffected by replacing BMLS with BSS+Glucose + AminoAcids for 30 min before Ins
    *** comment: bkg inhibited by replacing BMLS with BSS+Glucose - AminoAcids for 30 min before Ins
    *** comment: unaffected by replacing BMLS with BSS+Glucose + CHX for 30 min before Ins
    *** source: 15024086-Fig-4a
  *** Eef2k[Ab]IP phos(S78)[phosAb] is increased irt Ins (30 min)
    *** cells: KB in BMLS
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** unaffected by: U0126 [pretreatment]
    *** source: 15024086-Fig-2c
  *** Eef2k[Ab]IP phos(S78)[phosAb] is increased irt Ins (30 min)
    *** cells: KB in BMLS
    *** comment: bkg inhibited by replacing BMLS with BSS+Glucose - AminoAcids for 4 h4 before Ins
    *** comment: unaffected by replacing BMLS with BSS+Glucose + AminoAcids for 4 hr before Ins
    *** source: 15024086-Fig-4b
  *** Eef2k[Ab]IP phos(S78)[phosAb] is increased irt Ins (30 min)
    *** cells: KB in BMLS
    *** source: 15024086-Fig-1d
  *** Eef2k[Ab]IP phos(S78)[phosAb] is increased irt Ins (30 min)
    *** cells: KB in BMLS
    *** source: 15024086-Fig-6a
  *** Eef2k[Ab]IP phos(S78)[phosAb] is increased irt Ins (30 min)
    *** cells: mEFs in BMLS
    *** source: 15024086-Fig-5b
  *** xEef2k[tAb]IP phos(S78)[phosAb] is increased irt Ins (30 min)
    *** cells: KB<xEef2k> in BMLS
    *** source: 15024086-Fig-1e

========================
Phosphorylation on S359
========================

  *** Eef2k[Ab]IP phos(S359)[phosAb] is increased irt Ins (30 min)
    *** cells: KB in BMLS
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** unaffected by: U0126 [pretreatment]
    *** source: 15024086-Fig-2c
  *** Eef2k[Ab]IP phos(S359)[phosAb] is increased irt Ins (30 min)
    *** cells: KB in BMLS
    *** comment: unaffected by replacing BMLS with BSS+Glucose + AminoAcids for 4 hr before Ins
    *** comment: bkg inhibited by replacing BMLS with BSS+Glucose - AminoAcids for 4 h4 before Ins
    *** source: 15024086-Fig-4b
  *** xEef2k[tAb]IP phos(S359)[phosAb] is increased irt Ins (30 min)
    *** cells: KB<xEef2k> in BMLS
    *** unaffected by: xEef2k(S78A) [substitution]
    *** source: 15024086-Fig-1e

========================
Phosphorylation on S366
========================

  *** Eef2k[Ab]IP phos(S366)[phosAb] is increased irt Ins (30 min)
    *** cells: KB in BMLS
    *** unaffected by: Rapamycin [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** unaffected by: U0126 [pretreatment]
    *** source: 15024086-Fig-2c
  *** Eef2k[Ab]IP phos(S366)[phosAb] is unchanged irt Ins (30 min)
    *** cells: KB in BMLS
    *** source: 15024086-Fig-4b
  *** Eef2k[Ab]IP phos(S366)[phosAb] is unchanged irt Ins (30 min)
    *** cells: KB in BMLS
    *** source: 15024086-Fig-6a
  *** xEef2k[tAb]IP phos(S366)[phosAb] is unchanged irt Ins (30 min)
    *** cells: KB<xEef2k> in BMLS
    *** source: 15024086-Fig-1e


============
Other Sites
============

  *** xEef2k[tAb]IP phos(S377)[phosAb] is unchanged irt Ins (30 min)
    *** cells: KB<xEef2k> in BMLS
    *** source: 15024086-Fig-1e
  *** xEef2k[tAb]IP phos(S396)[phosAb] is unchanged irt Ins (30 min)
    *** cells: KB<xEef2k> in BMLS
    *** source: 15024086-Fig-1e

