===============
Kinase Activity
===============

not Akt1--

  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293 in BMS
    *** inhibited by: xAkt1(mnr)"CA" [addition]
    *** unaffected by: xAkt1(K179A)"DN" [addition]
    *** source: 10576742-Fig-1b

Braf-- ???

  *** xRaf1[tAb]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7 in BMS
    *** partially reqs: Braf [RNAi]
    *** source: 16364920-Fig-4b

Brap--  ???

  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HELA in BMS
    *** unaffected by: xBrap [addition]
    *** source: 14724641-Fig-2c

Gab1--

  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5++ 15+ 30+ min
    *** partially reqs: Gab1 [KO]
    *** source: 12629518(D)

Hras--

  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (20 min)
    *** cells: OT23 in BMS
    *** inhibited by: xHras(S17N)"DN" [addition]
    *** source: 11231577-Fig-3

not Mek12s-- ???

  *** xRaf1[tAb]IP IVKA(Mek1,Erk1)[32P-ATP] is increased irt Egf (30 min)
    *** cells: COS7 in BMLS
    *** unaffected by: U0126 [addition]
    *** source: 16093354-Fig-3b
  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (1 hr)
    *** cells: REF52 in BMS
    *** enhanced by: PD98059 [addition]
    *** enhanced by: U0126 [addition]
    *** source: 10480934-Fig-4a

not Pak1--

  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: NIH3T3 in BMLS
    *** unaffected by: xPak1(83-149)"DN" [addition]
    *** source: 16129686-Fig-6

Pi3K-- ???

  *** Raf1[Ab]IP IVKA(Mek?,Erk?,MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: COS in BMS
    *** times: 0 2 5+ 10++ 20+++ 60++++ min
    *** partially inhibited by: Wortmannin [addition]
    *** partially inhibited by: LY294002 [addition]
    *** source: 11259591-Fig-1b
  *** Raf1[Ab]IP phos(S338)[phosAb] is increased irt Egf (times)
    *** cells: COS in BMS
    *** times: 0 2+ 5+ 10+ 20+ 60+ min
    *** partially inhibited by: Wortmannin [addition]
    *** partially inhibited by: LY294002 [addition]
    *** source: 11259591-Fig-1a

Rap1a--

  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xRap1a(G12V)"CA" [addition]
    *** source: 9094716-Fig-5c

Shoc2--  ???

  *** xRaf1[tAb]IP IVKA(Mek1,Erk1,Elk1)[32P-ATP] is increased irt Egf (3 min)
    *** cells: HEK293 in BMLS
    *** enhanced by: xShoc2 [addition]
    *** source: 10783161-Fig-2c

chemicals

  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS1 in BMLS
    *** partially inhibited by: Okadaic-acid [addition]
    *** source: 10801873-Fig-2a,2b
  *** Raf1[Ab] infraction(membrane)[WB] is increased irt Egf (10 min)
    *** cells: COS1 in BMLS
    *** inhibited by: Okadaic-acid [addition]
    *** source: 11756411-Fig-3a
  *** xRaf1[tAb]IP IVKA(Mek1,Erk1,MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 2+ 5++ 10++ min
    *** partially inhibited by: Okadaic-acid [addition]
    *** source: 11756411-Fig-1c
  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: PC12<xPdgfRb> in BMLS
    *** bkg inhibited by: Forskolin [addition]
    *** source: 7935374-Fig-2b,2c
  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HELA in BMLS
    *** inhibited by: Forskolin + IBMX [addition]
    *** source: 16581779(D)

no extras

  *** Raf1[Ab]IP IVKA(Mek1)(SMANS)[phosAb] is increased irt Egf (5 min)
    *** cells: A431 in BMLS
    *** source: 19766101-Fig-S1
  *** Raf1[Ab]IP IVKA(Mek1)(SMANS)[phosAb] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 1+ 3++ 5++ 7++ 10++ 15+ 20+ 30 min
    *** source: 19766101-Fig-2c
  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: RAT1 in BMLS
    *** source: 9774335-Fig-3b
  *** Raf1[Ab]IP IVKA(Mek1,Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: bLUCs in BMLS
    *** source: 12644307-Fig-1a
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 2 5++ 10++ 20+ 40+ min
    *** source: 11782426-Fig-1d
  *** Raf1[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (20 min)
    *** cells: COS in BMS
    *** source: 10205168-Fig-4a
  *** Raf1[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2++ 5++ 10++ 20+ 60 min
    *** source: 11296227-Fig-4a
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7 in BMLS
    *** source: 12738796-Fig-1c
  *** Raf1[Ab]IP IVKA(Mek1,Erk1,MBP)[32P-ATP] is increased irt Egf (2 min)
    *** cells: HELA in BMS
    *** source: 8621729-Fig-5c
  *** Raf1[Ab]IP IVKA(Mek1,Erk1,MBP)[32P-ATP] is increased irt Egf (2 min)
    *** cells: HELA in BMS
    *** source: 8621729-Fig-6
  *** Raf1[Ab]IP IVKA(Mek2,Erk1,MBP)[32P-ATP] is increased irt Egf (2 min)
    *** cells: HELA in BMS
    *** source: 8621729-Fig-6
  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (times)
    *** cells: PC12 in BMS
    *** times: 0+ 1+++ 2+++ 5++ 10+ 20 45 90 180 min
    *** source: 8647237-Fig-1a
  *** Raf1[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: PC12 in BMLS
    *** source: 11278445-Fig-2
  *** Raf1[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0 2+++ 5++ 15+ 30 60 min
    *** source: 11278445-Fig-1a

Raf1 features

  --- phos(S29) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk1)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xRaf1(S29A) [substitution]
    *** source: 16093354-Fig-5,6a,6c,6d
  --- phos(S43) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk1)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xRaf1(S43A) [substitution]
    *** source: 16093354-Fig-5,6a,6c,6d
  --- phos(S259) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk1)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xRaf1(S259A) [substitution]
    *** source: 16093354-Fig-5,6a,6c,6d
  *** xRaf1[tAb]IP IVKA(Mek1,Erk1,MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 2+ 5++ 10++ min
    *** unaffected by: xRaf1(S259A) [substitution]
    *** source: 11756411-Fig-1c
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS1 in BMLS
    *** unaffected by: xRaf1(S259A) [substitution]
    *** source: 10801873-Fig-2a,2b
  --- phos(S338) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (20 min)
    *** cells: COS in BMLS
    *** inhibited by: xRaf1(S338A) [substitution]
    *** source: 10205168-Fig-4c
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased irt Egf (3 min)
    *** cells: HEK293 in BMS
    *** inhibited by: xRaf1(S338A) [substitution]
    *** source: 11447113-Fig-3c
  --- phos(S339) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (20 min)
    *** cells: COS in BMLS
    *** unaffected by: xRaf1(S339A) [substitution]
    *** source: 10205168-Fig-4c
  --- phos(Y340) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (20 min)
    *** cells: COS in BMLS
    *** unaffected by: xRaf1(Y340A) [substitution]
    *** source: 10205168-Fig-4c
  --- phos(Y341) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (20 min)
    *** cells: COS in BMLS
    *** inhibited by: xRaf1(Y341A) [substitution]
    *** source: 10205168-Fig-4c
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased irt Egf (3 min)
    *** cells: HEK293 in BMS
    *** partially inhibited by: xRaf1(Y341F) [substitution]
    *** source: 11447113-Fig-3c
  --- phos(Y340/Y341) ---
  *** Raf1[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+++ 5++ 10++ 20+ 60 min
    *** inhibited by: kRaf1(Y340F/Y341F) [substitution]
    *** source: 11296227-Fig-4b
  --- phos(S338/Y341) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased irt Egf (3 min)
    *** cells: HEK293 in BMS
    *** unaffected by: xRaf1(S338A/Y341F) [substitution]
    *** partially inhibited by: xRaf1(S338D/Y341D) [substitution]
    *** source: 11447113-Fig-3c
  --- phos(S471) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk1)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xRaf1(S471A) [substitution]
    *** inhibited by: xRaf1(S471C) [substitution]
    *** inhibited by: xRaf1(S471D) [substitution]
    *** inhibited by: xRaf1(S471T) [substitution]
    *** source: 16093354-Fig-5,6a,6c,6d
  *** xRaf1[tAb]IP IVKA(Mek1,Erk1)(TEY)[phosAb] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xRaf1(S471A) [substitution]
    *** inhibited by: xRaf1(S471C) [substitution]
    *** inhibited by: xRaf1(S471D) [substitution]
    *** inhibited by: xRaf1(S471T) [substitution]
    *** source: 16093354-Fig-6b,6d
  --- phos(T481) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk1)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xRaf1(S481A) [substitution]
    *** unaffected by: xRaf1(S481C) [substitution]
    *** unaffected by: xRaf1(S481D) [substitution]
    *** unaffected by: xRaf1(S481T) [substitution]
    *** source: 16093354-Fig-5,6a,6c,6d
  *** xRaf1[tAb]IP IVKA(Mek1,Erk1)(TEY)[phosAb] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xRaf1(S481A) [substitution]
    *** unaffected by: xRaf1(S481C) [substitution]
    *** unaffected by: xRaf1(S481D) [substitution]
    *** unaffected by: xRaf1(S481T) [substitution]
    *** source: 16093354-Fig-6b,6d
  --- phos(T491) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased irt Egf (3 min)
    *** cells: HEK293 in BMS
    *** unaffected by: xRaf1(T491A) [substitution]
    *** source: 11447113-Fig-1b
  --- phos(S494) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased irt Egf (3 min)
    *** cells: HEK293 in BMS
    *** unaffected by: xRaf1(S494A) [substitution]
    *** source: 11447113-Fig-1b
  --- phos(T491/S494) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased irt Egf (3 min)
    *** cells: HEK293 in BMS
    *** partially inhibited by: xRaf1(T491A/S494A) [substitution]
    *** source: 11447113-Fig-1b
  --- phos(S621) ---
  *** xRaf1[tAb]IP IVKA(Mek1,Erk1)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xRaf1(S621A/S259A) [substitution]
    *** unaffected by: xRaf1(S259A) [substitution]
    *** source: 16093354-Fig-5,6a,6c,6d


===============
Phosphorylation
===============

phos(T269)

  *** Raf1[Ab]IP phos(T269)[phosAb] is increased irt Egf (5 min)
    *** cells: A431 in BMLS
    *** source: 19766101-Fig-2a
  *** Raf1[Ab]IP phos(T269)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** source: 19766101-Fig-S2
  *** Raf1[Ab]IP phos(T269)[phosAb] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 1+ 3+ 5+ 7 10 15 20 30 min
    *** source: 19766101-Fig-2b
  *** Raf1[phosAb] phos(T269)[phosAb] is increased irt Egf (5 min)
    *** cells: A431 in BMLS
    *** inhibited by: xKsr1(D683A/D700A)"KD" [addition]
    *** source: 19766101-Fig-2d

phos(S259) decreased

  *** Raf1[phosAb] phos(S259)[phosAb] is decreased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0+++ 1++ 3+ 5+ 7+ 10+ 15+ 20+ 30+ min
    *** source: 19766101-Fig-2b
  *** xRaf1[tAb]IP phos(S259)[phosAb] is decreased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0+++ 2++ 5+ 10++ 20++ 40++ min
    *** source: 11782426-Fig-1e
  *** xRaf1[phosAb] phos(S259)[phosAb] is decreased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0++ 2+ 5+ 10+ min
    *** source: 11756411-Fig-1a,1b
  *** Raf1[phosAb] phos(S259)[phosAb] is decreased irt Egf (tnr)
    *** cells: HELA in BMLS
    *** source: 16041367-Fig-3c
  *** Raf1[Ab]IP phos(S259)[phosAb] is unchanged irt Egf (20 min)
    *** cells: COS in BMS
    *** source: 12077328-Fig-7a

phos(S338)

  *** Raf1[phosAb] phos(S338)[phosAb] is increased irt Egf (5 min)
    *** cells: PC6 in BMLS
    *** source: 15632082-Fig-1c
  *** Raf1[phosAb] phos(S338)[phosAb] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 1+ 3++ 5++ 7++ 10++ 15++ 20++ 30++ min
    *** source: 19766101-Fig-2b
  *** Raf1[phosAb] phos(S338)[phosAb] is increased irt Egf (times)
    *** cells: CHO<xEgfR><xInsR> in BMS
    *** times: 0 2+ 5+ 15+ min
    *** source: 15192046-Fig-5b
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased irt Egf (5 min)
    *** cells: HELA in BMS
    *** enhanced by: xBrap [addition]
    *** source: 14724641-Fig-2b
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased irt Egf (20 min)
    *** cells: COS in BMS
    *** inhibited by: xRaf1(R89L)"Ras-binding-domain-mut" [substitution]
    *** source: 11259591-Fig-8b
  *** Raf1[phosAb] phos(S338)[phosAb] is increased irt Egf (2 min)
    *** cells: HELA in BMS
    *** unaffected by: BaptaAM [addition]
    *** comment: phosAb also sees Braf-S446
    *** source: 16858395-Fig-5e
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 2 5++ 10++ 20+++ 40 min
    *** source: 11782426-Fig-1e
  *** Raf1[Ab]IP phos(S338)[phosAb] is increased irt Egf (20 min)
    *** cells: COS in BMLS
    *** source: 10205168-Fig-4b
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased irt Egf (20 min)
    *** cells: COS in BMS
    *** inhibited by: xRaf1(S338A) [substitution]
    *** unaffected by: xRaf1(Y341A) [substitution]
    *** unaffected by: xRaf1(S339A) [substitution]
    *** unaffected by: xRaf1(Y340A) [substitution]
    *** source: 10205168-Fig-5a
  *** Raf1[phosAb] phos(S338)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMLS
    *** times: 0 2+ 10+ min
    *** reqs: Phb [RNAi]
    *** source: 16041367-Fig-3b
  *** Raf1[phosAb] phos(S338)[phosAb] is increased irt Egf (tnr)
    *** cells: HELA in BMLS
    *** source: 16041367-Fig-3c
  *** Raf1[phosAb] phos(S338)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1+ 2+ 5+ 10+ 15+ min
    *** source: 15710605-Fig-1b

phos(T491/S494) increased

  *** Raf1[phosAb] phos(T491/S494)[phosAb] is increased irt Egf (10 min)
    *** cells: CCD18CO in BMLS
    *** does not req: Mlk3 [RNAi]
    *** source: 15258589-Fig-3b

phos(S621)

  *** xRaf1[phosAb] phos(S621)[phosAb] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0+ 2+ 5++ 10++ min
    *** source: 11756411-Fig-1a,1b

phos(S642) increased

  *** Raf1[phosAb]IP phos(S642)[phosAb] is increased irt Egf (times)
    *** cells: COS<xPin1> in BMS
    *** times: 0 30+ 240 min
    *** comment: decrease at 240 min inhibited by xPin1(K63A) [substitution]
    *** source: 15664191-Fig-5d
  *** Raf1[phosAb] phos(S642)[phosAb] is increased irt Egf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 30+ 240 min
    *** comment: prolonged by Okadaic-acid [addition]
    *** source: 15664191-Fig-5b

=====================
Protein Interactions
=====================

Braf--

  *** Raf1[Ab] copptby[WB] Braf[Ab] is increased irt Egf (5 min)
    *** cells: COS1 in BMLS
    *** source: 16508002-Fig-1b
  *** xRaf1[tAb] copptby[WB] xBraf[tAb] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0+ 2++ 5+++ 10++ 30++ 60+ 120+ min
    *** comment: xBraf(T753A) [substitution] inhibits decrease at 60-120 min
    *** source: 16508002-Fig-5e
  *** Raf1[Ab] copptby[WB] Braf[Ab] is increased irt Egf (times)
    *** cells: HELA in BMS "pretreated with U0126"
    *** times: 0 5+ 10++ min
    *** inhibited by: Brap [RNAi]
    *** source: 18332145-Fig-2c
  *** Raf1[Ab] copptby[WB] Braf[Ab] is increased irt Egf (times)
    *** cells: mEFs<Ksr1~null><xKsr1> in BMLS
    *** times: 0 5+ 10+ 15 30 min
    *** reqs: xKsr1 [omission]
    *** source: 18332145-Fig-4a

Hras--

  *** Raf1[Ab] copptby[WB] Hras[Ab] is increased irt Egf (10 min)
    *** cells: COS7 in BMS
    *** source: 16364920-Fig-2b
  *** Raf1[Ab] copptby[WB] Hras[Ab] is increased irt Egf (5 min)
    *** cells: HELA in BMLS
    *** reqs: Phb [RNAi]
    *** source: 16041367-Fig-4e
  *** Raf1[Ab] copptby[WB] Hras[Ab] is increased irt Egf (5 min)
    *** cells: HELA in BMLS
    *** source: 16041367-Fig-4f
  *** Raf1[Ab] copptby[WB] Hras[Ab] is detectable
    *** cells: HEK293T in BMS "treated with Egf (5 min)"
    *** does not req: Shoc2 [RNAi]
    *** comment: coppt Raf1 is not phosed on S259 [phosAb]
    *** comment: coppt Raf1 is phosed on S259 [phosAb] in Shoc2-KD [RNAi] cells
    *** source: 16630891-Fig-6d
  *** Raf1[Ab] snaggedby[WB] rHras{GMPPNP}[GST] is increased irt Egf (20 min)
    *** cells: PC12 in BMS
    *** bkg inhibited by: rHras{GDP} [substitution]
    *** source: 9001246-Fig-3b

Ksr1--

  *** Raf1[Ab] copptby[WB] xKsr1[tAb] is unchanged irt Egf (5 min)
    *** cells: mEFs<Ksr1~null><xKsr1> in BMS
    *** source: 2292131-Fig-1b

Pin1--

  *** Raf1[Ab]IP copptby[WB] Pin1[MS] is detectable
    *** cells: COS in BMS "treated with Egf (30 min)"
    *** source: 15664191-Fig-5b
  *** xRaf1[tAb] copptby[WB] xPin1(S16A)[tAb] is detectable
    *** cells: COS in BMS "treated with Egf (30 min)"
    *** inhibited by: xRaf1(S29A/S289A/S296A/S301A/S43A/S642A) [substitution]
    *** source: 15664191-Fig-5c

Ppp2cs--

  *** Raf1[Ab] copptby[WB] Ppp2cs[Ab] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 2+ 15++ min
    *** source: 11756411-Fig-2a

Pppcb--

  *** Raf1[Ab]IP copptby[WB] Ppp2cb[MS] is detectable
    *** cells: COS in BMS "treated with Egf (30 min)"
    *** source: 15664191-Fig-5b

Pppr1a--

  *** Raf1[Ab]IP copptby[WB] Ppp2r1a[MS] is detectable
    *** cells: COS in BMS "treated with Egf (30 min)"
    *** source: 15664191-Fig-5b

Pppr2a--

  *** Raf1[Ab]IP copptby[WB] Ppp2r2a[MS] is detectable
    *** cells: COS in BMS "treated with Egf (30 min)"
    *** source: 15664191-Fig-5b

Rheb--

  *** Raf1[Ab] snaggedby[WB] rRheb{GMPPNP}[GST] is detectable-but unchanged irt Egf (20 min)
    *** cells: PC12 in BMS
    *** inhibited by: rRheb{GDP} [substitution]
    *** source: 9001246-Fig-3b

========
Location
========

  *** xRaf1[Ab] infraction(membrane)[WB] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 2+ 10+ 15+ min
    *** inhibited by: xRaf1(S259A) [substitution]
    *** source: 11756411-Fig-4a
  *** Raf1[Ab] infraction(PM2)[WB] is increased irt Egf (3 min)
    *** cells: C3H10T12 in BMLS
    *** source: 11358964-Fig-1c


