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need evidence that Tpl2 copptby[WB] TnfR1 irt Tnf  is detectable
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not Ikk1--

  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 7+ 30++ min
    *** does not req: Ikk1 [KO]
    *** source: 15199157-Fig-5

Ikk2--

  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 7+ 30++ min
    *** reqs: Ikk2 [KO]
    *** source: 15199157-Fig-5

Nemo--

  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 7+ 30++ min
    *** reqs: Nemo [KO]
    *** source: 15199157-Fig-5

Ripk1--

  *** xTpl2[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: mEFs in BMS
    *** reqs: Ripk1 [KO]
    *** source: 15833743-Fig-3a
  *** Tpl2[Ab] copptby[WB] Ripk1[Ab] is increased irt Tnf (times)
    *** cells: mBMMs in BMLS
    *** times: 0 2 5+ min
    *** source: 16291755-Fig-2a

Syk--

  *** Tpl2[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Tnf (10 min)
    *** cells: mBMMs in BMLS
    *** inhibited by: Piceatannol [addition]
    *** source: 16291755-Fig-3e
  *** Tpl2[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Tnf (times)
    *** cells: JURKAT-H33HJ-JA1 in BMLS
    *** times: 0 10+ 15+ min
    *** reqs: Syk [null-mut]
    *** source: 16291755-Fig-7c
  *** Tpl2[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Tnf (tnr)
    *** cells: MCF7 in BMLS
    *** reqs: Syk [RNAi]
    *** source: 16291755-Fig-7f
  *** Tpl2[Ab]IP IVKA(Mek1)(S217/S221)[phosAb] is increased irt Tnf (times)
    *** cells: MCF7 in BMLS
    *** times: 0 10+ 15+ min
    *** enhanced by: xSyk [addition]
    *** source: 16291755-Fig-7f

Traf2--

  *** Tpl2[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Tnf (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 20++ min
    *** reqs: Traf2 [KO]
    *** source: 16291755-Fig-2f

chemicals

  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Tnf (15 min)
    *** cells: mBMMs in BMS
    *** inhibited by: PS1145 [pretreatment]
    *** source: 15199157-Fig-3c


no extras

  *** Tpl2[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Tnf (times)
    *** cells: HEK293 in BMS
    *** times: 0 10+ 20++ 30 min
    *** source: 16291755-Fig-3e
  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Tnf (times)
    *** cells: mBMMs in BMS
    *** times: 0 2 5+ 10++ 15++ 30 min
    *** source: 15199157-Fig-4


===============================
Release by degradation of Nfkb1
===============================

  *** Tpl2[Ab] copptby[WB] Nfkb1[Ab] is decreased irt Tnf (15 min)
    *** cells: mEFs in BMS
    *** comment: only Tpl2p58 band is decreased
    *** does not req: Ikk1 [KO]
    *** comment: decrease correlates with decrease in Nfkb1 prot-exp
    *** source: 15199157-Fig-6b
  *** Tpl2[Ab] copptby[WB] Nfkb1[Ab] is decreased irt Tnf (15 min)
    *** cells: mEFs in BMS
    *** comment: only Tpl2p58 band is decreased
    *** reqs: Ikk2 [KO]
    *** comment: decrease correlates with decrease in Nfkb1 prot-exp
    *** source: 15199157-Fig-6b
  *** Tpl2[Ab] copptby[WB] Nfkb1[Ab] is decreased irt Tnf (15 min)
    *** cells: mEFs in BMS
    *** comment: only Tpl2p58 band is decreased
    *** reqs: Nemo [KO]
    *** comment: decrease correlates with decrease in Nfkb1 prot-exp
    *** source: 15199157-Fig-6b
