===============================
Recruitment to the EgfR complex
===============================

  *** Shc1[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (10 min)
    *** cells: 16E6P in BMS "Egf deprived"
    *** comment: Shc1p52 is the major isoform recruited
    *** source: 8662998-Fig-4
  *** Shc1[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (2 min)
    *** cells: A7R5 in BMLS
    *** comment: an increased in two Shc1 bands are seen but no MWs are reported
    *** source: 11907028-Fig-13a
  *** Shc1[Ab] copptby[WB] xEgfR[Ab] is increased irt Egf (10 min)
    *** cells: NIH3T3<xErbb2> in BMLS
    *** comment: all three isoforms are recruited by p46 and p52 are predominant
    *** source: 9710588-Fig-3a
  *** Shc1[Ab] copptby[WB] xEgfR[Ab] is increased irt Egf (10 min)
    *** cells: NIH3T3<xErbb3> in BMLS
    *** source: 9710588-Fig-3a
  *** Shc1[Ab] copptby[WB] xEgfR[Ab] is increased irt Egf (10 min)
    *** cells: NIH3T3<xErbb4> in BMLS
    *** comment: all three isoforms are recruited by p46 and p52 are predominant
    *** source: 9710588-Fig-3a
  *** Shc1[Ab] copptby[WB] xEgfR[Ab] is increased irt Egf (10 min)
    *** cells: NIH3T3<xErbb4> in BMLS
    *** comment: all three isoforms are recruited by p46 and p52 are predominant
    *** source: 9710588-Fig-3b
  *** Shc1[Ab] copptby[WB] xEgfR[Ab] is increased irt Egf (times)
    *** cells: NIH3T3<xErbb4> in BMLS
    *** times: 0 1+ 5+ 10+ 15+ 30+ 60+ 120 min
    *** comment: all three isoforms are recruited by p46 and p52 are predominant
    *** source: 9710588-Fig-8a
  *** EgfR[Ab] copptby[WB] Shc1[Ab] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** source: 10330169-Fig-4b

==============================
Direct phosphorylation by EgfR
==============================

  *** rShc1p46[32P] phos[32P-ATP] is increased by pEgfR
    *** cells: none
    *** comment: pEgfR was purified by WGA-affinity + tyrosine-affinity
    *** partially inhibited by: rShc1p46(Y427F) [substitution]
    *** partially inhibited by: rShc1p46(Y349F/Y350F) [substitution]
    *** inhibited by: rShc1p46(Y349F/Y350F/Y427F) [substitution]
    *** source: 9388490-Fig-3

===========================================
Tyrosine phosphorylation in response to Egf
===========================================

Yphos-p66 --

  *** Shc1p66[Ab]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: 16E6P in BMS "Egf deprived"
    *** source: 8662998-Fig-4
  *** Shc1p66[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5+++ 15++ 30+ min
    *** does not req: Gab1 [KO]
    *** source: 12629518(D)
  *** Shc1p66[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: RAT1 in BMLS
    *** times: 0 1+ 2+ 5 10 20 min
    *** source: 7798267-Fig-1a
  *** Shc1p66[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: CHO<xInsR><xEgfR> in BMLS
    *** times: 0 1+++ 3++ 10++ 30+ 60+ min
    *** source: 8663461-Fig-3a
  *** Shc1p66[Ab]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: KB in BMLS
    *** source: 12009895-Fig-4
  *** Shc1p66[Ab]IP Yphos[32Pi-PAA] is increased irt Egf (2 min)
    *** cells: SWISS3T3 in BMS
    *** source: 17673906-Fig-2b

Yphos-p52 --

  *** Shc1p52[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5++ 15++ 30+ min
    *** does not req: Gab1 [KO]
    *** source: 12629518(D)
  *** Shc1p52[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: RAT1 in BMLS
    *** times: 0 1++ 2++ 5++ 10+ 20 min
    *** source: 7798267-Fig-1a
  *** Shc1p52[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: CHO<xInsR><xEgfR> in BMLS
    *** times: 0 1+++ 3+++ 10+++ 30++ 60+ min
    *** source: 8663461-Fig-3a
  *** Shc1p52[Ab]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: KB in BMLS
    *** source: 12009895-Fig-4
  *** xShc1p52[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: COS1 in BMLS
    *** inhibited by: xShc1(Y349F/Y350F) [substitution]
    *** unaffected by: xShc1(Y427F) [substitution]
    *** source: 8939605-Fig-7b
  *** xShc1p52[tAb]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: COS1 in BMLS
    *** partially inhibited by: xShc1(Y349F) [substitution]
    *** partially inhibited by: xShc1(Y350F) [substitution]
    *** inhibited by: xShc1(Y349F/Y350F) [substitution]
    *** source: 8939605-Fig-6b
  *** Shc1p52[Ab]IP Yphos[32Pi-PAA] is increased irt Egf (2 min)
    *** cells: SWISS3T3 in BMS
    *** source: 17673906-Fig-2b

Yphos-p46 --

  *** Shc1p46[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5+++ 15++ 30+ min
    *** does not req: Gab1 [KO]
    *** source: 12629518(D)
  *** Shc1p46[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: RAT1 in BMLS
    *** times: 0 1++ 2+  5+ 10 20 min
    *** source: 7798267-Fig-1a
  *** Shc1p46[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: CHO<xInsR><xEgfR> in BMLS
    *** times: 0 1+++ 3+++ 10+++ 30++ 60+ min
    *** source: 8663461-Fig-3a
  *** Shc1p46[Ab]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: KB in BMLS
    *** source: 12009895-Fig-4
  *** Shc1p46[Ab]IP Yphos[32Pi-PAA] is increased irt Egf (2 min)
    *** cells: SWISS3T3 in BMS
    *** source: 17673906-Fig-2b

Yphos-all or unknown --

  *** Shc1[MS] Yphos[pYAb-ppt] is increased irt Egf (5 min)
    *** cells: HELA in BMLS
    *** source: 10618391-Table-1
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: HACAT in BMLS
    *** source: 10969083-Fig-3c
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: NIH3T3<xEgfR> in BMS
    *** times: 0 1+ 15+ 30+ min
    *** source: 8626525-Fig-2c
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: COS1 in BMS
    *** source: 10508618-Fig-4
  *** xShc1[Ab]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: NIH3T3<xEgfR> in BMLS
    *** partially inhibited by: xShc1(Y427F) [substitution]
    *** partially inhibited by: xShc1(Y349F/Y350F) [substitution]
    *** inhibited by: xShc1(Y349F/Y350F/Y427F) [substitution]
    *** source: 9121430-Fig-3b
  *** Shc1[MS] Yphos[pYAb-ppt] is increased irt Egf (5 min)
    *** cells: HELA in BMLS
    *** comment: ppt Shc1 was predicted to be phosed on Y427
    *** source: 11687594-Table-1,2
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: HEK293 in BMLS
    *** source: 10675331-Fig-5b
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: CHO<xEgfR><xInsR> in BMS
    *** times: 0 2+ 5+ 15+ min
    *** comment: all three Shc1 isoforms were Yphosed
    *** source: 15192046-Fig-3a
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** unaffected by: LY294002 [pretreatment]
    *** source: 10330169-Fig-4c
  *** Shc1[Ab]IP Yphos[pYAb] is increased irt Egf (1 min)
    *** cells: PC12 in BMS
    *** source: 8288573-Fig-1

===========================================================
Physical interaction with other members of the EgfR complex
===========================================================


Grb2--

  *** Grb2[Ab] copptby[WB] xShc1p66[Ab] is increased irt Egf (5 min)
    *** cells: COS1 in BMS
    *** source: 9049300(D)
  *** Shc1p66[MS] snaggedby[WB] rGrb2(mnr)"SH2"[GST] is increased irt Egf (10 min)
    *** cells: HELA in BMLS
    *** source: 12577067(D)
  *** Shc1p66[Ab] copptby[WB] Grb2[Ab] is increased irt Egf (10 min)
    *** cells: 16E6P in BMS "Egf deprived"
    *** source: 8662998-Fig-4
  *** Grb2[Ab] copptby[WB] (xShc1p52[Ab] + xShc1p46[Ab]) is increased irt Egf (5 min)
    *** cells: COS1 in BMS
    *** source: 9049300(D)
  *** Shc1p46[Ab] copptby[WB] Grb2[Ab] is increased irt Egf (10 min)
    *** cells: 16E6P in BMS "Egf deprived"
    *** source: 8662998-Fig-4
  *** Shc1[Ab] copptby[WB] Grb2[Ab] is increased irt Egf (15 min)
    *** cells: NIH3T3<xEgfR> in BMS
    *** unaffected by: xEgfR(Y1016F/Y1092F/Y1110F/Y1172F/Y1197F) [substitution]
    *** source: 8626525-Fig-4b
  *** Shc1[Ab] copptby[WB] Grb2[Ab] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** unaffected by: Wortmannin [addition] no-bkg-control
    *** unaffected by: LY294002 [addition] no-bkg-control
    *** source: 11134009-Fig-5a
  *** Shc1[Ab] snaggedby[WB] Grb2[GST] is increased irt Egf (1 min)
    *** cells: PC12 in BMS
    *** source: 8288573-Fig-1

Cbl--

  *** Shc1[Ab] copptby[WB] Cbl[Ab] is increased irt Egf (times)
    *** cells: T47D in BMLS
    *** times: 0+ 2+++ 5+++ 10++ 20++ 30++ 60++ 120++ min
    *** source: 11994282(D)

Pik3c2b--

  *** Shc1[Ab] copptby[WB] xPik3c2b[tAb] is increased irt Egf (10 min)
    *** cells: A431 in BMLS "Triton-Soluble fraction"
    *** source: 16775008-Fig-2d
  *** Shc1p46[Ab] copptby[WB] xPik3c2b[tAb] is increased irt Egf (times)
    *** cells: A431 in BMLS "Triton-Insoluble fraction"
    *** times: 0 1+ 5+ 10+ 15+ 20+ 25+ 30+ 60+ 120+ min
    *** source: 16775008-Fig-1a
  *** Shc1p46[Ab] copptby[WB] xPik3c2b[tAb] is increased irt Egf (times)
    *** cells: A431 in BMLS "Triton-Soluble fraction"
    *** times: 0 1+ 5+ 10+ 15+ 20+ 25+ 30+ 60+ 120+ min
    *** source: 16775008-Fig-1a
  *** Shc1p52[Ab] copptby[WB] xPik3c2b[tAb] is increased irt Egf (times)
    *** cells: A431 in BMLS "Triton-Insoluble fraction"
    *** times: 0 1+ 5+ 10+ 15+ 20+ 25+ 30+ 60+ 120+ min
    *** source: 16775008-Fig-1a
  *** Shc1p52[Ab] copptby[WB] xPik3c2b[tAb] is increased irt Egf (times)
    *** cells: A431 in BMLS "Triton-Soluble fraction"
    *** times: 0 1+ 5+ 10+ 15+ 20+ 25+ 30+ 60+ 120+ min
    *** source: 16775008-Fig-1a
  ----------------------------------------------------------------------------
  *** Shc1[Ab]IP IVLKA(PtdIns/PI4P/PI45P2)[TLC] is increased irt Egf (5 min)
    *** cells: A431<xPik3c2b> in BMLS
    *** source: 16775008-Fig-3e


==========
other phos
==========

  *** Shc1p46[Ab]IP Sphos[32Pi-PAA] is unchanged irt Egf (2 min)
    *** cells: SWISS3T3 in BMS
    *** source: 17673906-Fig-2b
  *** Shc1p52[Ab]IP Sphos[32Pi-PAA] is unchanged irt Egf (2 min)
    *** cells: SWISS3T3 in BMS
    *** source: 17673906-Fig-2b
  *** Shc1p66[Ab]IP Sphos[32Pi-PAA] is unchanged irt Egf (2 min)
    *** cells: SWISS3T3 in BMS
    *** source: 17673906-Fig-2b
  *** Shc1p46[Ab]IP phos[32Pi] is increased irt Egf (2 min)
    *** cells: SWISS3T3 in BMS
    *** times: 0+ 5+ 10++ min
    *** source: 17673906-Fig-2a
  *** Shc1p52[Ab]IP phos[32Pi] is increased irt Egf (2 min)
    *** cells: SWISS3T3 in BMS
    *** times: 0+ 5+ 10++ min
    *** source: 17673906-Fig-2a
  *** Shc1p66[Ab]IP phos[32Pi] is increased irt Egf (2 min)
    *** cells: SWISS3T3 in BMS
    *** source: 17673906-Fig-2a


