=============================
Pras40-Mtor required Proteins
=============================

not Mlst8--

  *** Pras40[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** does not req: Mlst8 [RNAi]
    *** source: 17510057-Fig-2a

Raptor--

  *** xPras40[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293T<xMtor><xRaptor><xPras40><xMlst8> in BMS
    *** reqs: xRaptor [omission]
    *** source: 17510057-Fig-2b
  *** xPras40[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293T<xMtor><xRaptor><xPras40> in BMS
    *** reqs: xRaptor [omission]
    *** source: 17510057-Fig-2b
  *** Pras40[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** partially reqs: Raptor [RNAi]
    *** source: 17510057-Fig-2a
  *** Pras40[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293E in BMLS
    *** reqs: Raptor [RNAi]
    *** source: 17386266-Fig-3f
  *** xPras40[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293E<xPras40><xMtor> in BMLS
    *** enhanced by: xRaptor [addition]
    *** source: 17386266-Fig-3e
  *** Pras40[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** reqs: Raptor [RNAi]
    *** source: 17277771-Fig-1f


not Rictor--

  *** Pras40[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** does not req: Rictor [RNAi]
    *** source: 17510057-Fig-2a

====================================
Pras40-Mtor required Pras40 features
====================================

  *** xMtor[tAb] copptby[WB] xPras40[tAb] is detectable
    *** cells: HEK293T<xMtor><xRaptor><xPras40> in BMS
    *** unaffected by: xPras40(P77H/P78A) [substitution]
    *** unaffected by: xPras40(P84A/P85A) [substitution]
    *** unaffected by: xPras40(P93A/P94A) [substitution]
    *** inhibited by: xPras40(F129A) [substitution]
    *** unaffected by: xPras40(T160A/P161A) [substitution]
    *** partially inhibited by: xPras40(S183A) [substitution]
    *** partially inhibited by: xPras40(P185A) [substitution]
    *** unaffected by: xPras40(F193A) [substitution]
    *** unaffected by: xPras40(S202A/S203A) [substitution]
    *** unaffected by: xPras40(T246A) [substitution]
    *** unaffected by: xPras40(F249A) [substitution]
    *** source: 17510057-Fig-4b
  *** Mtor[Ab] copptby[WB] xPras40[tAb] is detectable
    *** cells: HEK293E<xPras40> in BMS
    *** inhibited by: xPras40(S183A) [substitution]
    *** partially inhibited by: xPras40(S185A) [substitution]
    *** unaffected by: xPras40(S212A) [substitution]
    *** enhanced by: xPras40(S221A) [substitution]
    *** enhanced by: xPras40(S246A) [substitution]
    *** source: 18372248-Fig-5
  *** Raptor[Ab] copptby[WB] xPras40[tAb] is detectable
    *** cells: HEK293E<xPras40> in BMS
    *** inhibited by: xPras40(S183A) [substitution]
    *** partially inhibited by: xPras40(S185A) [substitution]
    *** unaffected by: xPras40(S212A) [substitution]
    *** enhanced by: xPras40(S221A) [substitution]
    *** enhanced by: xPras40(S246A) [substitution]
    *** source: 18372248-Fig-5
  *** Mtor[Ab] copptby[WB] xPras40[tAb] is detectable
    *** cells: HEK293T<xPras40><xS6k1> in BMS
    *** inhibited by: xPras40(1-150) [substitution]
    *** unaffected by: xPras40(103-258) [substitution]
    *** inhibited by: xPras40(142-258) [substitution]
    *** inhibited by: xPras40(150-258) [substitution]
    *** unaffected by: xPras40(1-234) [substitution]
    *** inhibited by: xPras40(150-234) [substitution]
    *** source: 17277771-Fig-2d

==================================
Pras40-Mtor required Mtor features
==================================

  *** xPras40[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293T<xPras40><xMtor> in BMS
    *** inhibited by: xMtor(1-1482) [substitution]
    *** partially inhibited by: xMtor(1348-2549) [substitution]
    *** unaffected by: xMtor(2000-2548) [substitution]
    *** source: 17277771-Fig-1c

============================
Pras40-Mtor lysis conditions
=============================

Pras40-Mtor  TritonX not OK

  *** xPras40[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293T<xMtor><xPras40><xRaptor> in BMS
    *** comment: inhibited by replacement of Tween20 by TritonX in lysis buffer
    *** source: 17510057-Fig-2c
  *** Mtor[Ab] snaggedby[WB] rPras40[GST] is detectable
    *** cells: HEK293T in BMS
    *** comment: inhibited by replacement of Tween20 by TritonX in lysis buffer
    *** source: 17510057-Fig-S1b
  *** Pras40[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: inhibited by use of TritonX instead of Chaps in lysis buffer
    *** source: 17277771-Fig-S3c


===============================
Pras40-Raptor required Proteins
===============================

not Mlst8--

  *** Pras40[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** does not req: Mlst8 [RNAi]
    *** source: 17510057-Fig-2a

not Mtor-- ???

  *** Pras40[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293E in BMLS
    *** does not req: Mtor [RNAi]
    *** source: 17386266-Fig-3f
  ---------------------------------------------------
  *** Pras40[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** reqs: Mtor [RNAi]
    *** source: 17510057-Fig-2a

not Rictor--

  *** Pras40[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** does not req: Rictor [RNAi]
    *** source: 17510057-Fig-2a


=======================================
Pras40-Raptor required Pras40 features
=======================================

  *** xRaptor[tAb] copptby[WB] xPras40[tAb] is detectable
    *** cells: HEK293T<xMtor><xRaptor><xPras40> in BMS
    *** unaffected by: xPras40(P77H/P78A) [substitution]
    *** unaffected by: xPras40(P84A/P85A) [substitution]
    *** unaffected by: xPras40(P93A/P94A) [substitution]
    *** inhibited by: xPras40(F129A) [substitution]
    *** unaffected by: xPras40(T160A/P161A) [substitution]
    *** partially inhibited by: xPras40(S183A) [substitution]
    *** partially inhibited by: xPras40(P185A) [substitution]
    *** unaffected by: xPras40(F193A) [substitution]
    *** unaffected by: xPras40(S202A/S203A) [substitution]
    *** unaffected by: xPras40(T246A) [substitution]
    *** unaffected by: xPras40(F249A) [substitution]
    *** source: 17510057-Fig-4b
  *** xRaptor[tAb] copptby[WB] xPras40[tAb] is detectable
    *** cells: HEK293T<xMtor><xPras40><xRaptor> in BMS
    *** comment: ppts were washed with 0.2% TritonX
    *** unaffected by: xPras40(P77H/P78A) [substitution]
    *** unaffected by: xPras40(P84A/P85A) [substitution]
    *** unaffected by: xPras40(P93A/P94A) [substitution]
    *** inhibited by: xPras40(F129A) [substitution]
    *** unaffected by: xPras40(T160A/P161A) [substitution]
    *** inhibited by: xPras40(S183A) [substitution]
    *** inhibited by: xPras40(P185A) [substitution]
    *** unaffected by: xPras40(F193A) [substitution]
    *** unaffected by: xPras40(S202A/S203A) [substitution]
    *** unaffected by: xPras40(T246A) [substitution]
    *** unaffected by: xPras40(F249A) [substitution]
    *** source: 17510057-Fig-4b
  *** Raptor[Ab] copptby[WB] xPras40[tAb] is detectable
    *** cells: HEK293T<xPras40><xS6k1> in BMS
    *** inhibited by: xPras40(1-150) [substitution]
    *** unaffected by: xPras40(103-258) [substitution]
    *** inhibited by: xPras40(142-258) [substitution]
    *** inhibited by: xPras40(150-258) [substitution]
    *** unaffected by: xPras40(1-234) [substitution]
    *** inhibited by: xPras40(150-234) [substitution]
    *** source: 17277771-Fig-2d


==============================
Pras40-Raptor lysis conditions
===============================

Pras40-Raptor  TritonX not OK ???

  *** xPras40[tAb] copptby[WB] xRaptor[tAb] is detectable
    *** cells: HEK293T<xMtor><xPras40><xRaptor> in BMS
    *** comment: unaffected by replacement of Tween20 by TritonX in lysis buffer
    *** source: 17510057-Fig-2c
  *** Raptor[Ab] snaggedby[WB] rPras40[GST] is detectable
    *** cells: HEK293T in BMS
    *** comment: inhibited by replacement of Tween20 by TritonX in lysis buffer
    *** source: 17510057-Fig-S1b
  *** Pras40[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: inhibited by use of TritonX instead of Chaps in lysis buffer
    *** source: 17277771-Fig-S3d

no extras

  *** xPras40[tAb] copptby[WB] xRaptor[tAb] is detectable
    *** cells: HEK293T<xMtor><xRaptor><xPras40> in BMS
    *** source: 17510057-Fig-1c
  *** Pras40[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17510057-Fig-1b
  *** Pras40[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17510057-Fig-1b
  *** xPras40[tAb] copptby[WB] xRaptor[tAb] is detectable
    *** cells: HEK293E<xRaptor><xPras40><xMtor> in BMLS
    *** source: 17386266-Fig-3e
  *** xPras40[tAb] copptby[WB] xRaptor[tAb] is detectable
    *** cells: HEK293E<xRaptor><xPras40><xTuba?> in BMLS
    *** source: 17386266-Fig-3e
  *** Pras40[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-1a
  *** Pras40[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-1b
  *** Pras40[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** reqs: Mtor [RNAi]
    *** source: 17277771-Fig-1f
  *** Pras40[Ab] copptby[WB] Raptor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-1b
  *** Pras40[Ab] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293T<xMtor> in BMS
    *** source: 17277771-Fig-S3a
  *** Mtor[Ab] copptby[WB] xPras40[tAb] is detectable
    *** cells: HEK293T<xPras40> in BMS
    *** source: 17277771-Fig-S3b


