===========================
Semi-direct Phosphorylation
===========================

fragments

  *** rTsc1(301-600)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** inhibited by: rTsc1(1-300) [substitution]
    *** inhibited by: rTsc1(601-900) [substitution]
    *** inhibited by: rTsc1(901-1164) [substitution]
    *** source: 17693255-Fig-S7a
  *** rTsc1(301-600)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 17693255-Fig-S7a
  *** rTsc1(401-500)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** comment: phos-site is shown to be S487 by MS
    *** source: 17693255-Fig-S8a
  *** rTsc1(401-500)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** inhibited by: rTsc1(301-400) [substitution]
    *** partially inhibited by: rTsc1(501-600) [substitution]
    *** source: 17693255-Fig-S7b
  *** rTsc1(401-500)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 17693255-Fig-S7b
  *** rTsc1(401-500)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** inhibited by: xIkk2(S487A) [substitution]
    *** inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 17693255-Fig-4f
  *** rTsc1(501-600)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** comment: phos-site is shown to be S511 by MS
    *** source: 17693255-Fig-S8b
  *** rTsc1(501-600)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** inhibited by: xIkk2(S487A) [substitution]
    *** inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 17693255-Fig-4f

on S487 ???

  *** xTsc1[Ab]IP phos(S487)[phosAb] is detectable
    *** cells: MCF7<xTsc1> in BMS
    *** reqs: Ikk2 [RNAi]
    *** source: 17693255-Fig-S9a
  *** rTsc1[phosAb] phos(S487)[phosAb] is increased by xIkk2[tAb]IP
    *** cells: none
    *** inhibited by: rTsc1(S487A/S511A) [substitution]
    *** source: 17693255-Fig-4g
  *** rTsc1[phosAb] phos(S487)[phosAb] is increased by xIkk2[tAb]IP
    *** cells: none
    *** inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 17693255-Fig-4g
  *** rTsc1[phosAb] phos(S487)[phosAb] is increased by xIkk2[tAb]IP
    *** cells: none
    *** inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 17693255-Fig-4h
  *** rTsc1[phosAb] phos(S487)[phosAb] is unchanged by xIkk1[tAb]IP
    *** cells: none
    *** source: 17693255-Fig-4h

on S511

  *** xTsc1[Ab]IP phos(S511)[phosAb] is detectable
    *** cells: MCF7<xTsc1> in BMS
    *** reqs: Ikk2 [RNAi]
    *** source: 17693255-Fig-S9a
  *** rTsc1[phosAb] phos(S511)[phosAb] is increased by xIkk2[tAb]IP
    *** cells: none
    *** inhibited by: rTsc1(S487A/S511A) [substitution]
    *** source: 17693255-Fig-4g
  *** rTsc1[phosAb] phos(S511)[phosAb] is increased by xIkk2[tAb]IP
    *** cells: none
    *** inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 17693255-Fig-4g
  *** rTsc1[phosAb] phos(S511)[phosAb] is increased by xIkk2[tAb]IP
    *** cells: none
    *** inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 17693255-Fig-4h
  *** rTsc1[phosAb] phos(S511)[phosAb] is unchanged by xIkk1[tAb]IP
    *** cells: none
    *** source: 17693255-Fig-4h

other

  *** xTsc1[tAb] upshift[WBMS] is increased itpo xIkk2
    *** cells: HEK293<xTsc1> in BMS
    *** comment: upshift was reversed by CIAP treatment
    *** source: 17693255-Fig-S4
