=============
Transcription
=============

not Ask1--

  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (16 hr)
    *** cells: THP1 in BMS
    *** unaffected by: xAsk1(mnr)"DN" [addition]
    *** source: 18155167-Fig-4d

Jun-- ???

  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (12 hr)
    *** cells: CHANG in BMS
    *** inhibited by: xJun(mnr)"DN" [addition] no-bkg-control
    *** source: 17451682-Fig-4a
  *** Hif1a-gene promo-reporter[Luc] is increased irt Lps (12 hr)
    *** cells: CHANG in BMS
    *** inhibited by: xJun(mnr)"DN" [addition] no-bkg-control
    *** source: 17451682-Fig-4b

chemicals

  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (12 hr)
    *** cells: CHANG in BMS
    *** inhibited by: SP600125 [pretreatment]
    *** unaffected by: PD98059 [pretreatment]
    *** unaffected by: SB203580 [pretreatment]
    *** unaffected by: LY294002 [pretreatment]
    *** source: 17451682-Fig-2b
  *** Hif1a-gene promo-reporter[Luc] is increased irt Lps (12 hr)
    *** cells: CHANG in BMS
    *** partially inhibited by: SP600125 [pretreatment]
    *** unaffected by: PD98059 [pretreatment]
    *** unaffected by: SB203580 [pretreatment]
    *** unaffected by: LY294002 [pretreatment]
    *** source: 17451682-Fig-2c
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (3 hr)
    *** cells: THP1 in BMS
    *** inhibited by: PD98059 [pretreatment]
    *** source: 16533170-Fig-5a
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (4 hr)
    *** cells: THP1 in BMS
    *** bkg inhibited by: ActinomycinD [addition]
    *** source: 16533170-Fig-3c
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (tnr)
    *** cells: THP1 in BMS
    *** inhibited by: Bortezomib [addition]
    *** source: 16533170-Fig-6c

no extras

  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (12 hr)
    *** cells: CHANG in BMS
    *** source: 17451682-Fig-1a
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (12 hr)
    *** cells: CHANG in BMS
    *** source: 17451682-Fig-3b
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (12 hr)
    *** cells: HELA in BMS
    *** source: 17451682-Fig-1a
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (12 hr)
    *** cells: HEPG2 in BMS
    *** source: 17451682-Fig-1a
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (12 hr)
    *** cells: HUH7 in BMS
    *** source: 17451682-Fig-1a
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (times)
    *** cells: CHANG in BMS
    *** times: 0+ 2+ 4++ 8+++ 10+++ 12+++ hr
    *** source: 17451682-Fig-1b
  *** Hif1a-gene mRNA[RTPCR] is unchanged irt Lps (12 hr)
    *** cells: U937 in BMS
    *** source: 17451682-Fig-1a
  *** Hif1a-gene promo-reporter[Luc] is increased irt Lps (12 hr)
    *** cells: CHANG in BMS
    *** source: 17451682-Fig-1c
  *** Hif1a-gene promo-reporter[Luc] is increased irt Lps (12 hr)
    *** cells: CHANG in BMS
    *** source: 17451682-Fig-3a
  *** Hif1a-gene promo-reporter[Luc] is increased irt Lps (12 hr)
    *** cells: HEPG2 in BMS
    *** source: 17451682-Fig-1c
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (3 hr)
    *** cells: hMDMacs in BMS
    *** source: 16533170-Fig-2c
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (3 hr)
    *** cells: THP1 in BMS
    *** source: 16533170-Fig-1b
  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (3 hr)
    *** cells: THP1 in BMS "treated for 5 days with PMA"
    *** source: 16533170-Fig-2b
  *** Hif1a-gene mRNA[Northern] is increased irt Lps (6 hr)
    *** cells: NR8383 in BMLS
    *** source: 14525767-Fig-3a
  *** Hif1a-gene mRNA[Northern] is increased irt Lps (times)
    *** cells: NR8383 in BMLS
    *** times: 0+ 2+ 4+ 6++ 8++ hr
    *** source: 14525767-Fig-3a
  *** Hif1a-gene mRNA[Northern] is increased irt Lps (3 hr)
    *** cells: hSNFs in BMLS
    *** source: 10926858-Fig-2

===========
Translation
===========

  *** Hif1a-gene mRNA[RTPCR] is increased irt Lps (12 hr)
    *** cells: CHANG in BMS
    *** unaffected by: CHX [pretreatment]
    *** source: 17451682-Fig-2a

==================
Protein Expression
==================

Ask1--

  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (4 hr)
    *** cells: THP1 in BMS
    *** inhibited by: xAsk1(mnr)"DN" [addition]
    *** source: 18155167-Fig-4a

Chemicals

  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (12 hr)
    *** cells: CHANG in BMS
    *** inhibited by: SP600125 [pretreatment]
    *** partially inhibited by: PD98059 [pretreatment]
    *** partially inhibited by: SB203580 [pretreatment]
    *** unaffected by: LY294002 [pretreatment]
    *** source: 17451682-Fig-2d
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (6 hr)
    *** cells: NR8383 in BMLS
    *** partially inhibited by: LY294002 [pretreatment] no-bkg-control
    *** inhibited by: Bisindolylmaleimide-I [pretreatment] no-bkg-control
    *** source: 14525767-Fig-5a
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (6 hr)
    *** cells: NR8383 in BMLS
    *** inhibited by: ActinomycinD [pretreatment] no-bkg-control
    *** source: 14525767-Fig-4a
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (6 hr)
    *** cells: THP1 in BMS
    *** unaffected by: NMMA [pretreatment]
    *** inhibited by: Genistein [pretreatment] no-bkg-control
    *** inhibited by: U73122 [pretreatment] no-bkg-control
    *** inhibited by: Go6983 [pretreatment] no-bkg-control
    *** inhibited by: DPI [pretreatment] no-bkg-control
    *** inhibited by: SB203580 [pretreatment] no-bkg-control
    *** source: 18155167-Fig-3
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (3 hr)
    *** cells: THP1 in BMS
    *** inhibited by: PD98059 [pretreatment]
    *** source: 16533170-Fig-5b
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (4 hr)
    *** cells: THP1 in BMS
    *** bkg inhibited by: ActinomycinD [addition]
    *** source: 16533170-Fig-3d
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (4 hr)
    *** cells: THP1 in BMS
    *** bkg inhibited by: CHX [addition]
    *** source: 16533170-Fig-3b
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (4 hr)
    *** cells: THP1 in BMS
    *** enhanced by: MG132 [addition]
    *** source: 16533170-Fig-3b

no extras

  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (times)
    *** cells: CHANG in BMS
    *** times: 0+ 1+ 2++ 4++ hr
    *** source: 17451682-Fig-2e
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (6 hr)
    *** cells: mBMMs in BMLS
    *** source: 14525767-Fig-2a
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (6 hr)
    *** cells: NR8383 in BMLS
    *** source: 14525767-Fig-2a
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (6 hr)
    *** cells: NR8383 in BMLS
    *** source: 14525767-Fig-2b
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (times)
    *** cells: NR8383 in BMLS
    *** times: 0+ 15+ 30+ 60+ 120++ 180++ 240+++ 360+++ 480++++ hr
    *** source: 14525767-Fig-2c
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (6 hr)
    *** cells: THP1 in BMS
    *** inhibited by: anti-TLR4 [pretreatment] no-bkg-control
    *** source: 18155167-Fig-3
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (6 hr)
    *** cells: THP1 in BMS
    *** source: 18155167-Fig-1a
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (times)
    *** cells: THP1 in BMS
    *** times: 0+ 1+ 2++ 4++ 8++ 16++ 32++ 64++ hr
    *** source: 18155167-Fig-1c
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (times)
    *** cells: THP1 in BMS
    *** times: 0+ 15+ 30+ 45++ 60++ min
    *** source: 18155167-Fig-1b
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (4 hr)
    *** cells: hMDMacs in BMS
    *** source: 16533170-Fig-2c
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (4 hr)
    *** cells: THP1 in BMS "treated for 5 days with PMA"
    *** source: 16533170-Fig-2b
  *** Hif1a[Ab] prot-exp[WB] is increased irt Lps (times)
    *** cells: THP1 in BMS
    *** times: 1 2+ 4++ 8++ 16+ hr
    *** source: 16533170-Fig-1a


==================================================
Oligo-binding (correlates with Protein-expression)
==================================================

  *** Hif1a[Ab] oligo-binding[ELISA] is increased irt Lps (6 hr)
    *** cells: NR8383 in BMLS "nuclear-extracts"
    *** source: 14525767-Fig-6a
  *** Hif1a[Ab] oligo-binding[EMSA] is increased irt Lps (tnr)
    *** cells: THP1 in BMS "nuclear-extracts"
    *** source: 16533170-Fig-1c

============
HRE Reporter
============

  *** HRE-reporter[Luc] is increased irt Lps (6 hr)
    *** cells: RAW2647 in BMS
    *** source: 19762779-Fig-3f
  *** HRE-reporter[Luc] is increased irt Lps (24 hr)
    *** cells: RAW2647 in BMS
    *** source: 19762779-Fig-3f
