======================
Direct Phosphorylation
======================

  *** rTp53[32P] phos[32P-ATP] is increased by rHipk2
    *** cells: none
    *** inhibited by: xHipk2(K221R) [substitution]
    *** source: 11780126-Fig-4a
  *** rTp53[32P] phos[32P-ATP] is increased by rHipk2
    *** cells: none
    *** inhibited by: rHipk2(K221A) [substitution]
    *** unaffected by: rHipk2(1-520)"delC" [substitution]
    *** source: 11740489-Fig-5b

==========================
SemiDirect Phosphorylation
==========================

S46

  *** rTp53[32P] phos[32P-ATP] is increased by xHipk2[Ab]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS
    *** partially inhibited by: xTp53(S46A) [substitution]
    *** source: 11740489-Fig-S1c
  *** sTp53(25-65)[phosAb] phos(S46)[phosAb] is increased by xHipk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS 
    *** source: 11780126-Fig-4d
  *** rTp53[phosAb] phos(S46)[phosAb] is increased by xHipk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS 
    *** source: 11780126-Fig-4e

not S6

  *** sTp53(1-39)[phosAb] phos(S6)[phosAb] is unchanged by xHipk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS 
    *** source: 11780126-Fig-4d

not S9

  *** sTp53(1-39)[phosAb] phos(S9)[phosAb] is unchanged by xHipk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS 
    *** source: 11780126-Fig-4d

not S15

  *** sTp53(1-39)[phosAb] phos(S15)[phosAb] is unchanged by xHipk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS 
    *** source: 11780126-Fig-4d
  *** rTp53[phosAb] phos(S15)[phosAb] is unchanged by xHipk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS 
    *** source: 11780126-Fig-4e

not S20

  *** sTp53(1-39)[phosAb] phos(S20)[phosAb] is unchanged by xHipk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS 
    *** source: 11780126-Fig-4d

not S33

  *** sTp53(1-39)[phosAb] phos(S33)[phosAb] is unchanged by xHipk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS 
    *** source: 11780126-Fig-4d

not S37

  *** sTp53(1-39)[phosAb] phos(S37)[phosAb] is unchanged by xHipk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS 
    *** source: 11780126-Fig-4d

total

  *** rTp53[32P] phos[32P-ATP] is increased by xHipk2[Ab]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS
    *** inhibited by: xHipk2(K221A) [substitution]
    *** source: 11740489-Fig-S1a
  *** rTp53(1-80)[32P] phos[32P-ATP] is increased by xHipk2[Ab]IP
    *** cells: none
    *** IPfrom: HEK293<xHipk2> in BMS
    *** inhibited by: xHipk2(K221A) [substitution]
    *** source: 11740489-Fig-S1b

==================================
Phosphorylation in the presence of
==================================

not S20

  *** xTp53[phosAb] phos(S20)[phosAb] is unchanged itpo xHipk2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** unaffected by: xHipk2(K221A) [substitution]
    *** source: 11740489-Fig-5a

S46

  *** xTp53[phosAb] phos(S46)[phosAb] is increased itpo xHipk2
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** inhibited by: xHipk2(K221A) [substitution]
    *** source: 11740489-Fig-5a
  *** xTp53[Ab]IP phos(S46)[phosAb] is detectable
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** enhanced by: xHipk2 [addition]
    *** unaffected by: xHipk2(K221R) [addition]
    *** source: 11780126-Fig-4f
  *** Tp53[phosAb] phos(S46)[phosAb] is detectable
    *** cells: MCF7 in BMS "treated with UV for 12 hr"
    *** partially reqs: Hipk2 [AntiSense]
    *** source: 11780126-Fig-5d


===================
Protein Association
===================

  *** rHipk2[35S] boundby[WB] rTp53[GST] is detectable
    *** cells: none
    *** inhibited by: rHipk2(1-520)"delC" [substitution]
    *** unaffected by: rHipk2(551-1191)"delN" [substitution]
    *** unaffected by: rHipk2(K221A) [substitution]
    *** source: 11740489-Fig-3a
  *** rHipk2[35S] boundby[WB] rTp53[GST] is detectable
    *** cells: none
    *** inhibited by: rTp53(1-298) [substitution]
    *** unaffected by: rTp53(294-393) [substitution]
    *** source: 11740489-Fig-3b
  *** rHipk2[35S] boundby[WB] rTp53(13-390)[GST] is detectable
    *** cells: none
    *** unaffected by: rTp53(295-390) [substitution]
    *** inhibited by: rTp53(13-295) [substitution]
    *** source: 11780126-Fig-1f
  *** rTp53[35S] boundby[WB] rHipk2[GST] is detectable
    *** cells: none
    *** unaffected by: rHipk2(853-955) [substitution]
    *** inhibited by: rHipk2(1-853) [substitution]
    *** source: 11780126-Fig-1e

  *** Tp53[Ab] copptby[WB] Hipk2[Ab] is detectable
    *** cells: U2OS in BMS
    *** source: 11740489-Fig-3d
  *** Tp53[Ab] copptby[WB] Hipk2[Ab] is detectable
    *** cells: U2OS in BMS "treated with UV + As2O3"
    *** source: 11740489-Fig-3d
  *** Tp53[Ab] copptby[WB] xHipk2[tAb] is detectable
    *** cells: HEK293<xHipk2> in BMS
    *** inhibited by: xHipk2(1-520)"delC" [substitution]
    *** unaffected by: xHipk2(551-1191)"delN" [substitution]
    *** source: 11740489-Fig-3c
  *** Tp53[Ab] copptby[WB] xHipk2[tAb] is detectable
    *** cells: HEK293<xHipk2> in BMS
    *** source: 11780126-Fig-1g
  *** Tp53[Ab] copptby[WB] xHipk2[tAb] is detectable
    *** cells: HEK293<xHipk2> in BMS
    *** unaffected by: xHipk2(K221R) [substitution]
    *** comment: coppt Tp53 is phosed [32P-ATP]
    *** source: 11780126-Fig-4b



