==============================
Y705 Phosphorylation itpo xSrc
==============================

requires Fak2--

  *** xStat3[phosAb] phos(Y705)[phosAb] is increased itpo xSrc(Y530F)"CA"
    *** cells: HELA in BMS
    *** inhibited by: xFak2(K457A/D567A)"DN" [addition]
    *** enhanced by: xFak2 [addition]
    *** source: 14963038-Fig-2b

No extras

  *** xStat3[phosAb] phos(Y705)[phosAb] is increased itpo xSrc
    *** cells: COS1 in BMS
    *** source: 11278353-Fig-6a
  *** xStat3[Ab]IP phos(Y705)[phosAb] is increased itpo xSrc
    *** cells: SF9 in BMS
    *** inhibited by: xStat3(Y705F) [substitution]
    *** source: 12244095-Fig-1
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased itpo xSrc(mnr)"vSrc"
    *** cells: HEK293T in BMS
    *** source: 11598209-Fig-3a
  *** rStat3[pYAb] Yphos[pYAb] is increased by xSrc[tAb]IP
    *** cells: COS1 in BMS
    *** source: 11278353-Fig-4d
  *** xStat3[Ab]IP Yphos[pYAb] is increased itpo xSrc
    *** cells: SF9 in BMS
    *** partially inhibited by: xStat3(Y705F) [substitution]
    *** source: 12244095-Fig-1
  *** Stat3[Ab]IP Yphos[pYAb] is increased itpo xSrc(mnr)"vSrc"
    *** cells: REF in BMS
    *** source: 8657134-Fig-2c

===============================
Y705 Phosphorylation itpo xFak2
===============================

requires Fak2 kinase activity

  *** xStat3[phosAb] phos(Y705)[phosAb] is increased itpo xFak2
    *** cells: HELA in BMS
    *** inhibited by: xFak2(K457A/D567A)"KD" [substitution]
    *** source: 14963038-Fig-1b

==============================
S727 Phosphorylation itpo xSrc
==============================

requires Fak2--

  *** xStat3[phosAb] phos(S727)[phosAb] is increased itpo xSrc(Y530F)"CA"
    *** cells: HELA in BMS
    *** partially inhibited by: xFak2(K457A/D567A)"DN" [addition]
    *** enhanced by: xFak2 [addition]
    *** source: 14963038-Fig-2b

===============================
S727 Phosphorylation itpo xFak2
===============================

requires Fak2 kinase activity

  *** xStat3[phosAb] phos(S727)[phosAb] is increased itpo xFak2
    *** cells: HELA in BMS
    *** inhibited by: xFak2(K457A/D567A)"KD" [substitution]
    *** source: 14963038-Fig-1b

===============
Coprecipitation
===============

  *** xStat3[Ab] copptby[WB] xFak2[Ab] is detectable
    *** cells: HELA<xSrc(T529F)"CA"> in BMS
    *** source: 14963038-Fig-3
  *** xStat3[tAb] copptby[WB] xSrc(Y530F)"CA"[Ab] is detectable
    *** cells: HELA<xFak2> in BMS
    *** source: 14963038-Fig-3
  *** xSrc(mnr)"vSrc"[Ab] snaggedby[WB] rStat3[GST] is detectable
    *** cells: REF<xSrc(mnr)"vSrc"> in BMS
    *** source: 8657134-Fig-3a
  *** rSrc[Ab] boundby[WB] rStat3[GST] is detectable
    *** cells: none
    *** source: 8657134-Fig-4b
  *** Stat3[Ab] copptby[WB] xSrc(mnr)"vSrc"[Ab] is detectable
    *** cells: REF<xSrc(mnr)"vSrc"> in BMS
    *** source: 8657134-Fig-3a

===========
DNA-binding 
===========

  *** xStat3[Ab] oligo-binding[EMSA] is unchanged itpo xSrc
    *** cells: COS7 in BMS
    *** source: 9373245-Fig-2a
  *** Stat3[Ab] oligo-binding[EMSA] is increased itpo xSrc(mnr)"vSrc"
    *** cells: REF in BMS "nuclear-extracts"
    *** source: 8657134-Fig-2e

=================
Reporter activity
=================

  *** Stat3-reporter[Luc] is increased itpo xFak2
    *** cells: HELA<xStat3> in BMS
    *** source: 14963038-Fig-1a,6
  *** Stat3-reporter[Luc] is increased itpo xSrc(Y530F)"CA"
    *** cells: HELA<xStat3> in BMS
    *** inhibited by: xFak2(K457A/D567A)"DN" [addition]
    *** enhanced by: xFak2 [addition]
    *** source: 14963038-Fig-2a

=====================
Relocation to nucleus
=====================

  *** Stat3[Ab] infraction(nuclear)[WB] is increased itpo xSrc(mnr)"vSrc"
    *** cells: REF in BMS
    *** source: 8657134-Fig-2d


