=================
Required Proteins
=================

IL2Rb--

  *** xGab2[tAb]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: HEK293T<xIL2Ra><xIL2Rb><xIL2Rg><xJak3> in BMS
    *** partially inhibited by: xIL2Rb(Y338F) [substitution]
    *** source: 10849428-Fig-4a

Jak3--

  *** xGab2[tAb]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: HEK293T<xIL2Ra><xIL2Rb><xIL2Rg><xJak3> in BMS
    *** reqs: xJak3 [omission]
    *** source: 10849428-Fig-7d

not Jak1--

  *** xGab2[tAb]IP Yphos[pYAb] is unchanged irt IL2 (5 min)
    *** cells: HEK293T<xIL2Ra><xIL2Rb><xIL2Rg><xJak1> in BMS
    *** source: 10849428-Fig-7d

not Lck1--

  *** xGab2[tAb]IP Yphos[pYAb] is unchanged irt IL2 (5 min)
    *** cells: HEK293T<xIL2Ra><xIL2Rb><xIL2Rg><xLck> in BMS
    *** source: 10849428-Fig-7d

=================================
Gab2 Features required for Yphos
=================================

  *** xGab2[tAb]IP phos[WBMS] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "starved"
    *** partially inhibited by: xGab2(S623A) [substitution]
    *** source: 15356145-Fig-3
  *** xGab2[tAb]IP Yphos[pYAb] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** partially inhibited by: xGab2(Y614F) [substitution]
    *** source: 15170389-Fig-2a
  *** xGab2[tAb]IP Yphos[pYAb] is unchanged irt IL2 (5 min)
    *** cells: HEK293T<xIL2Ra><xIL2Rb><xIL2Rg> in BMS
    *** source: 10849428-Fig-7d


=========
No Extras
=========

  *** Gab2[Ab]IP phos[WBMS-PPase] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "starved"
    *** source: 15356145-Fig-1a 
  *** Gab2[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: hPBTCs in BMLS
    *** source: 10849428-Fig-1a
  *** Gab2[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: hPBTCs in BMLS
    *** source: 10849428-Fig-1b
  *** Gab2[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: NK33 in BMLS
    *** source: 10849428-Fig-1c
  *** Gab2[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: NK33 in BMLS
    *** source: 10849428-Fig-2
  *** Gab2[Ab]IP Yphos[pYAb] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "starved"
    *** source: 15356145-Fig-1a 


=========
Chemicals
=========

LY294002--

  *** Gab2[Ab]IP phos[WBMS] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "starved"
    *** partially inhibited by: LY294002 [pretreatment]
    *** source: 15356145-Fig-1a 

PP2--

  *** Gab2[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: NK33 in BMLS
    *** unaffected by: PP2 [pretreatment]
    *** source: 10849428-Fig-7a

PD98059--

  *** Gab2[Ab]IP phos[WBMS] is increased irt IL2 (times)
    *** cells: KIT225 in BMS "starved"
    *** times: 0 1 5+ min
    *** unaffected by: PD98059 [pretreatment]
    *** source: 15356145-Fig-1a 

SB203580--

  *** Gab2[Ab]IP phos[WBMS] is increased irt IL2 (times)
    *** cells: KIT225 in BMS "starved"
    *** times: 0 1 5+ min
    *** unaffected by: SB203580 [pretreatment]
    *** source: 15356145-Fig-1a 

U0126--

  *** Gab2[Ab]IP phos[WBMS] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "starved"
    *** partially inhibited by: U0126 [pretreatment]
    *** source: 15356145-Fig-1a 
  *** Gab2[Ab]IP phos[WBMS] is increased irt IL2 (times)
    *** cells: KIT225 in BMS "starved"
    *** times: 0 1 5+ min
    *** unaffected by: U0126 [pretreatment]
    *** source: 15356145-Fig-1a 
  *** Gab2[Ab]IP Yphos[pYAb] is increased irt IL2 (times)
    *** cells: KIT225 in BMS "starved"
    *** times: 0 5++ 10++ 30++ 60++ 90+ min
    *** comment: Gab2 mobility shift but not pY intensity inhibited by U0126
    *** source: 15356145-Fig-4


========
Location
========

not Pik3rs--

  *** Gab2[Ab] copptby[WB] Pik3rs[Ab] is unchanged irt IL2 (5 min)
    *** cells: NK33 in BMLS
    *** source: 10849428-Fig-2

Shp2--

  *** Gab2[Ab] copptby[WB] Shp2[Ab] is increased irt IL2 (5 min)
    *** cells: NK33 in BMLS
    *** source: 10849428-Fig-2
  *** xGab2[Ab] copptby[WB] Shp2[Ab] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** inhibited by: xGab2(Y614F) [substitution]
    *** source: 15170389-Fig-2b
  *** Gab2[Ab] copptby[WB] Shp2[Ab] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** source: 15170389-Fig-2b
  *** xGab2[Ab] copptby[WB] Shp2[Ab] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "starved"
    *** enhanced by: xGab2(S623A) [substitution]
    *** source: 15356145-Fig-5
  *** xGab2[Ab] copptby[WB] Shp2[Ab] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "starved"
    *** unaffected by: U0126 [pretreatment]
    *** source: 15356145-Fig-5
  *** Gab2[Ab] copptby[WB] Shp2[Ab] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "starved"
    *** unaffected by: U0126 [pretreatment]
    *** source: 15356145-Fig-5b

