===============
Kinase Activity
===============

inhibited by Dok2--

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: COS1 in BMS
    *** times: 0 2+ 5++ 10++ min
    *** partially inhibited by: xDok2 [addition]
    *** source: 10508618-Fig-3a

inhibited by Dusp1--

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xDusp1 [addition]
    *** source: 10473620-Fig-4c

inhibited by Dusp6--

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xDusp6 [addition]
    *** source: 10473620-Fig-4d

EgfR-- phos sites

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293<xEgfR> in BMLS
    *** unaffected by: xEgfR(Y1148F) [substitution]
    *** unaffected by: xEgfR(Y1173F) [substitution]
    *** unaffected by: xEgfR(Y954F/Y1153F) [substitution]
    *** source: 9733788-Fig-5f

reqs Hras--

  *** xErk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: mEFs in BMLS
    *** inhibited by: xHras(S17N)"DN" [addition]
    *** source: 11524436-Fig-3a
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7<xErk2> in BMLS
    *** partially inhibited by: xHras(S17N) [addition]
    *** source: 10330169-Fig-1c

Gabs--

Requirement for Gab1 phos(Y627/Y659) or Gab2 phos(Y614/Y643)
for Erk activation
~/evidence/Egf-Evidence/196.Gabs.txt

reqs IqGap1--

  *** Erks[phosAb]IP IVKA(Elk1)(sitenr)[phosAb] is increased irt Egf (10 min)
    *** cells: MCF7 in BMS
    *** partially inhibited by: xIqGap1 [addition]
    *** bkg reqs: IqGap1 [RNAi]
    *** source: 14970219-Fig-4a

reqs Mek1--

  *** xErk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: REF52 in BMS
    *** partially inhibited by: xMek1(K97A)"KD" [addition]
    *** source: 10480934-Fig-3d
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xMek1(mnr)"DN" [addition]
    *** source: 10473620-Fig-4e

not Mek5--

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xMek5(S311A/T315A)"DN" [addition]
    *** source: 10473620-Fig-4e

not Mekk1--

  *** Erk2[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS in BMLS
    *** inhibited by: xMekk1(K1274M)"DN" [addition] no-bkg-control
    *** source: 9305638-Fig-4e

not Mekk2--

  *** Erk2[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS in BMLS
    *** unaffected by: xMekk2(K385M)"DN" [addition]
    *** source: 9305638-Fig-4e

not Mekk3--

  *** Erk2[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS in BMLS
    *** unaffected by: xMekk3(K391M)"DN" [addition]
    *** source: 9305638-Fig-4e

not Mekk4--

  *** Erk2[Ab]IP IVKA(EgfR)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS in BMLS
    *** unaffected by: xMekk4(K1371M)"DN" [addition]
    *** source: 9305638-Fig-4e

not Mkk4--

  *** xErk1[tAb]IP IVKA(auto)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xMkk4(S220A/T224L)"DN" [addition]
    *** source: 12738796-Fig-3b


inhibited by Mlk3--  ???   scaffold???

  *** xErk1[tAb]IP IVKA(auto)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7<xErk1> in BMLS
    *** inhibited by: xMlk3 [addition]
    *** source: 12738796-Fig-3b
  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7<xErk1> in BMLS
    *** inhibited by: xMlk3 [addition]
    *** source: 12738796-Fig-3a
  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7<xErk1> in BMLS
    *** inhibited by: xMlk3 [addition]
    *** source: 12738796-Fig-1a

reqs Pi3k--

  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xPik3r?(mnr)"DN" [addition] no-bkg-control
    *** partially inhibited by: xPik3ca(K802R)"KD" [addition] no-bkg-control
    *** partially inhibited by: xPik3cb(K805R)"KD" [addition] no-bkg-control
    *** source: 11134009-Fig-2a
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7<xErk2> in BMLS
    *** bkg inhibited by: xPik3r?(mnr)"DN" [addition]
    *** source: 10330169-Fig-1b

not Pten--

  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xPten [addition] no-bkg-control
    *** unaffected by: xPten(G129E) [addition] no-bkg-control
    *** source: 11134009-Fig-3

inhibited by Ptpn6--

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293<xEgfR> in BMLS
    *** inhibited by: xPtpn6 [addition]
    *** source: 9733788-Fig-5f

does not req Raf1--

  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (10 min)
    *** cells: mEFs in BMLS
    *** does not req: Raf1 [KO]
    *** unaffected by: kRaf1(Y340F/Y341F) [substitution]
    *** source: 11296227-Fig-5d
  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2++ 5++ 10++ 20++ 60+ min
    *** does not req: Raf1 [KO]
    *** unaffected by: kRaf1(Y340F/Y341F) [substitution]
    *** source: 11296227-Fig-5c

not RapGef1--

  *** xErk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0+ 5++ 10+ 20+ 30+ 40+ 60+ min
    *** unaffected by: xRapGef1(mnr)"Crk-binding-mut" [addition]
    *** source: 9560161-Fig-5b

RasGrf1-- ???

  *** xErk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xRasGrf1 [addition]
    *** source: 11285260-Fig-1c

reqs Shc1--  ???

  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HELA in BMS
    *** enhanced by: xShc1p52 + xShc1p46 [addition]
    *** unaffected by: xShc1p66(1-110)"CH2-domain" [addition]
    *** source: 9049300-Fig-9b

not Sos1--

  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: mEFs<xEgfR> in BMLS
    *** times: 0 5++ 15++ 90 360 min
    *** does not req: Sos1 [KO]
    *** source: 10675333-Fig-7c
  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: mEFs<xEgfR> in BMLS
    *** does not req: Sos1 [KO]
    *** source: 10675333-Fig-6b
  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** unaffected by: xSos1 [addition]
    *** source: 11134009-Fig-4d

reqs Shoc2--  ???

  *** xErk1[tAb]IP IVKA(Elk1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** enhanced by: xShoc2 [addition]
    *** source: 10783161-Fig-1c

Tec--  ???

  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xTec(mnr)"PHDomain" [addition] no-bkg-control
    *** unaffected by: xTec(mnr/R29C)"PIP3-binding-mutant" [addition] no-bkg-control
    *** source: 11134009-Fig-3

Usp6nl--  ????

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: COS7 in BMS
    *** times: 0 15++ 30+ min
    *** unaffected by: xUsp6nl [addition]
    *** source: 11099046-Fig-4b

Erk2 features

  *** xErk2[tAb]IP IVKA(Fos)[32P-ATP] is increased irt Egf (5 min)
    *** cells: NIH3T3<xErk2> in BMLS
    *** inhibited by: xErk2(L198A) [substitution]
    *** inhibited by: xErk2(L232A) [substitution]
    *** inhibited by: xErk2(Y261A) [substitution]
    *** unaffected by: xErk2(D319N) [substitution]
    *** source: 16051177-Fig-1c
  *** xErk2[tAb]IP IVKA(Rsk?)[32P-ATP] is increased irt Egf (5 min)
    *** cells: NIH3T3<xErk2> in BMLS
    *** unaffected by: xErk2(L198A) [substitution]
    *** unaffected by: xErk2(L232A) [substitution]
    *** unaffected by: xErk2(Y261A) [substitution]
    *** partially inhibited by: xErk2(D319N) [substitution]
    *** source: 16051177-Fig-1b

Chemicals

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7<xErk2> in BMLS
    *** bkg inhibited by: LY294002 [pretreatment]
    *** source: 10330169-Fig-1b
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7<xErk2> in BMLS
    *** partially inhibited by: LY294002 [pretreatment]
    *** source: 10330169-Fig-1c


No extras--

  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: MDCK in BMLS
    *** times: 0 5++ 15++ 30+ 60+ 120+ 180 240 min
    *** source: 9668053-Fig-1b

=====================
Total Phosphorylation
=====================

  *** xErk2[tAb]IP phos[WBMS] is increased irt Egf (10 min)
    *** cells: HEK293T in BMLS
    *** source: 8631302-Fig-5
  *** Erks[Ab] phos[WBMS] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: LY294002 [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 10330169-Fig-1a



