===============
coppt with EgfR
===============

  *** xPlcg1[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (times)
    *** cells: mEFs<Plcg1~null><xPlcg1> in BMLS
    *** times: 0 5+ 15+ min
    *** unaffected by: xPlcg1(del(SH3)) [substitution]
    *** source: 12061819-Fig-3b

=======================
Phosphorylation on Y783
=======================

Git1--  ???

  *** xPlcg1[Ab]IP phos(Y783)[phosAb] is increased irt Egf (times)
    *** cells: HEK293<xGit1> in BMLS
    *** times: 0 2+ 10+ min
    *** partially inhibited by: xGit1(del(250-420))"Spa-domain-mut" [substitution]
    *** source: 14523024-Fig-6b,6c

not Gnai1-- or Gnai3--

  *** Plcg1[phosAb] phos(Y783)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+++ 5++ 15+ 30+ min
    *** does not req: Gnai1 or Gnai3 [KO]
    *** source: 19401591-Fig-2d

reqs Shp2--

  *** Plcg1[Ab]IP phos(Y783)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs<Shp2~null><xShp2> in BMLS
    *** reqs: xShp2 [omission]
    *** source: 14967142-Fig-2a
  *** Plcg1[Ab]IP phos(Y783)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** partially reqs: Shp2 [KO]
    *** source: 14967142-Fig-3b

reqs Src--

  *** Plcg1[phosAb] phos(Y783)[phosAb] is increased irt Egf (tnr)
    *** cells: mEFs<Src~null><Yes1~null><Fyn~null><xSrc> in BMLS
    *** reqs: xSrc [omission]
    *** source: 12845332-Fig-1d

no extras

  *** xPlcg1[Ab]IP phos(Y783)[phosAb] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** source: 14523024-Fig-3c
  *** xPlcg1[Ab]IP phos(Y783)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 2+ 10+ min
    *** source: 14523024-Fig-6a
  *** Plcg1[phosAb] phos(Y783)[phosAb] is increased irt Egf (times)
    *** cells: MTLN3 in BMLS
    *** times: 0 10 20+ 30+ 40++ 50++ 60++ 120+ 180+ sec
    *** source: 15337778-Fig-2b

===========================
Phosphorylation on Tyrosine
===========================

does not req Pld2--

  *** Plcg1[Ab]IP Yphos[pYAb] is increased irt Egf (1 min)
    *** cells: COS7 in BMS
    *** unaffected by: xPld2 [addition]
    *** unaffected by: xPld2(P145L/P148L) [addition]
    *** source: 12646582-Fig-7
  *** Plcg1[Ab]IP Yphos[pYAb] is increased irt Egf (1 min)
    *** cells: COS7<xPld2> in BMS
    *** does not req: xPld2 [omission]
    *** unaffected by: xPld2(P145L/P148L) [substitution]
    *** source: 12646582-Fig-7

No extras

  *** Plcg1[Ab]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: mEFs<Src~null><Yes1~null><Fyn~null><xSrc> in BMLS
    *** source: 14967142-Fig-2c
  *** xPlcg1[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: mEFs<Plcg1~null><xPlcg1> in BMLS
    *** times: 0 5+ 15+ min
    *** unaffected by: xPlcg1(del(SH3)) [substitution]
    *** source: 12061819-Fig-3a
  *** Plcg1[Ab] Yphos[pYAb-ppt] is increased irt Egf (times)
    *** cells: MTLN3 in BMLS
    *** times: 0 20+ 40++ 60+++ 80++ 120+ 180+ sec
    *** source: 15337778-Fig-2a
  *** Plcg1[MS] Yphos[pYAb-ppt] is increased irt Egf (times)
    *** cells: HELA in BMLS
    *** times: 0 1+++ 5++ 10+ 20+ min
    *** source: 15314609(D)
  *** Plcgs[Ab] Yphos[pYAb-ppt] is increased irt Egf (times)
    *** cells: HELA in BMLS
    *** times: 0 1++ 5+ 10 20 min
    *** source: 15314609(D)
  *** Plcgs[Ab]IP Yphos[pYAb] is unchanged irt Egf (5 min)
    *** cells: hUVECs in BMLS
    *** source: 10671553-Fig-6a
  *** pPlcg1[pYAb] Yphos[pYAb] is increased by pEgfR
    *** cells: none
    *** source: 11226410(D)

========================
Phosphorylation on S1248
========================

  *** Plcg1[phosAb] phos(S1248)[phosAb] is increased irt Egf (times)
    *** cells: COS7 in BMLS
    *** times: 0 15+ 30+ 120+ min
    *** source: 16525023-Fig-6a
  *** Plcg1[phosAb] phos(S1248)[phosAb] is increased irt Egf (tnr)
    *** cells: COS7 in BMLS
    *** enhanced by: xAkt1 [addition]
    *** inhibited by: xAkt1(P424A/P467A) [addition]
    *** source: 16525023-Fig-6b
  *** Plcg1[phosAb] phos(S1248)[phosAb] is increased irt Egf (tnr)
    *** cells: COS7 in BMLS
    *** inhibited by: Wortmannin [pretreatment]
    *** inhibited by: H89 [pretreatment]
    *** inhibited by: KT5720 [pretreatment]
    *** source: 16525023-Fig-6d
  *** Plcg1[phosAb] phos(S1248)[phosAb] is increased irt Egf (tnr)
    *** cells: COS7 in BMLS
    *** inhibited by: Wortmannin [pretreatment]
    *** unaffected by: U73122 [pretreatment]
    *** source: 16525023-Fig-6b

===========================
Relocation to cell membrane
===========================

  *** xPlcg1[XFP] locatedin(cellmembrane)[IHC] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 5+ 15+ min
    *** unaffected by: xPlcg1(del(SH3)) [substitution]
    *** source: 12061819-Fig-6a


==================================
Semidirect phosphorylation by EgfR
==================================

  *** rPlcg1(540-852)"SH2-SH2-PP-SH3" phos[32P-ATP] is increased by EgfR[Ab]IP
    *** cells: none
    *** IPfrom: A431 in BMS
    *** source: 1315766-Fig-4
  *** rPlcg1(540-852)"SH2-SH2-PP-SH3" phos[32P-ATP] is increased by EgfR[Ab]IP
    *** cells: none
    *** IPfrom: A431 in BMS
    *** unaffected by: rPlcg1(540-797)"SH2-SH2-PP" [substitution]
    *** partially inhibited by: rPlcg1(760-852)"PP-SH3" [substitution]
    *** unaffected by: rPlcg1(651-852)"SH2-PP-SH3" [substitution]
    *** inhibited by: rPlcg1(540-763)"SH2-SH2" [substitution]
    *** partially inhibited by: rPlcg1(760-797)"PP" [substitution]
    *** source: 1315766-Fig-5

======================================================
Coprecipitation with other members of the EgfR Complex
======================================================

Akts--

  *** Plcg1[Ab] copptby[WB] Akts[Ab] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** inhibited by: U73122 [pretreatment]
    *** source: 16525023-Fig-2b
  *** Plcg1[Ab] copptby[WB] Akts[Ab] is increased irt Egf (30 min)
    *** cells: COS7 in BMLS
    *** source: 16525023-Fig-2a
  *** Plcg1[Ab] copptby[WB] Akts[Ab] is increased irt Egf (times)
    *** cells: COS7 in BMLS
    *** times: 0 5+ 15++ 30+++ 60++ 120 min
    *** source: 16525023-Fig-2b
  *** xPlcg1[tAb] copptby[WB] Akts[Ab] is increased irt Egf (30 min)
    *** cells: COS7<xPlcg1> in BMLS
    *** source: 16525023-Fig-3b
  *** xPlcg1[tAb] copptby[WB] Akts[Ab] is increased irt Egf (30 min)
    *** cells: COS7<xPlcg1> in BMLS
    *** source: 16525023-Fig-5
