Rationale for this happening in the CHR?  because it reqs Atm??
    
=======================
Phosphorylation on S343
=======================

reqs Atm--

  *** Nbs1[Ab]IP phos(S343)[phosAb] is unchanged irt IR (30 min)
    *** cells: hLymphoblasts<Atm~fmut><xAtm> in BMS
    *** reqs: xAtm [omission]
    *** source: 10766245-Fig-4c
  *** xNbs1[tAb]IP phos(S343)[phosAb] is increased irt IR (30 min)
    *** cells: HEK293T<xNbs1><xAtm> in BMS
    *** inhibited by: xAtr(D2870A/N2875K)"KD" [substitution]
    *** source: 10766245-Fig-4b
  *** Nbs1[Ab]IP phos(S343)[phosAb] is unchanged irt IR (30 min)
    *** cells: GM1526<Atm~fmut> in BMS
    *** source: 10766245-Fig-4c
  *** Nbs1[phosAb] phos(S343)[phosAb] is unchanged irt IR (30 min)
    *** cells: GM9607<Atm~fmut> in BMS
    *** source: 15175241-Fig-S1a
  *** Nbs1[Ab]IP phos(S343)[phosAb] is unchanged irt IR (15 min)
    *** cells: AT3BI<Atm~fmut> in BMS
    *** source: 15234984-Fig-3a
  *** Nbs1[Ab]IP phos(S343)[phosAb] is unchanged irt IR (60 min)
    *** cells: AT3BI<Atm~fmut> in BMS
    *** source: 15234984-Fig-3b
  *** Nbs1[phosAb] phos(S343)[phosAb] is increased irt IR (10 min)
    *** cells: AT1ABR<Atm~fmut><xAtm> in BMS
    *** partially inhibited by: xAtm(S367A) [substitution]
    *** partially inhibited by: xAtm(S1983A) [substitution]
    *** partially inhibited by: xAtm(S1981A) [substitution]
    *** source: 16858402-Fig-5b

reqs Brca1--

  *** Nbs1[Ab]IP phos(S343)[phosAb] is increased irt IR (tnr)
    *** cells: HCC1937<Brca1~fmut><xBrca1> in BMS
    *** reqs: xBrca1 [omission]
    *** source: 15175241-Fig-4c

not Mdc1--

  *** Nbs1[phosAb] phos(S343)[phosAb] is increased irt IR (times)
    *** cells: NotReported in BMS
    *** times: 0 1+ 2+ hr
    *** does not req: Mdc1 [RNAi]
    *** source: 12607005-Fig-5e
  *** Nbs1[phosAb] phos(S343)[phosAb] is increased irt IR (10 min)
    *** cells: U2OS in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 15201865-Fig-4a
  *** Nbs1[phosAb] phos(S343)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 16377563-Fig-S5a

Mre11--

  *** Nbs1[Ab]IP phos(S343)[phosAb] is increased irt IR (15 min)
    *** cells: D6809<Mre11~fmut><xMre11> in BMS
    *** reqs: xMre11 [omission]
    *** unaffected by: xMre11(NLS) [substitution]
    *** source: 16478990-Fig-4b

not Smc1a--

  *** Nbs1[Ab] phos[WBMS-PPase] is increased irt IR (30 min)
    *** cells: mEFs in BMS
    *** unaffected by: Smc1a(S957A/S966A) [knockin]
    *** source: 15175241-Fig-5c

inhibited by Trf2--

  *** Nbs1[phosAb] phos(S343)[phosAb] is increased irt IR (45 min)
    *** cells: IMR90 in BMS
    *** partially inhibited by: xTrf2 [addition]
    *** source: 15314656-Fig-2c

Nbs1 features

  *** xNbs1[Ab]IP phos(S343)[phosAb] is increased irt IR (60 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** unaffected by: xNbs1(1-652) [substitution]
    *** source: 15234984-Fig-3b
  *** xNbs1[Ab]IP phos(S343)[phosAb] is increased irt IR (15 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** unaffected by: xNbs1(1-652) [substitution]
    *** source: 15234984-Fig-3a
  *** xNbs1[phosAb] phos(S343)[phosAb] is increased irt IR (times)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** times: 0 15+ 30++ 60++ min
    *** inhibited by: xNbs1(1-734)"delC" [substitution]
    *** source: 15758953-Fig-3a

no extras

  *** Nbs1[Ab]IP phos(S343)[phosAb] is increased irt IR (30 min)
    *** cells: GM536 in BMS
    *** source: 10766245-Fig-4c
  *** xNbs1[tAb]IP phos(S343)[phosAb] is increased irt IR (30 min)
    *** cells: HEK293T<xNbs1> in BMS
    *** source: 10766245-Fig-4b
  *** xNbs1[tAb]IP phos(S343)[phosAb] is increased irt IR (30 min)
    *** cells: HEK293T<xNbs1><xAtm> in BMS
    *** inhibited by: xNbs1(S343A) [substitution]
    *** source: 10766245-Fig-4b
  *** Nbs1[Ab]IP phos(S343)[phosAb] is increased irt IR (15 min)
    *** cells: 1070SK in BMS
    *** source: 15175241-Fig-1a
  *** Nbs1[phosAb] phos(S343)[phosAb] is increased irt IR (30 min)
    *** cells: GM637 in BMS
    *** source: 15175241-Fig-S1a
  *** Nbs1[Ab]IP phos(S343)[phosAb] is increased irt IR (15 min)
    *** cells: GM637 in BMS
    *** source: 15234984-Fig-3a
  *** Nbs1[Ab]IP phos(S343)[phosAb] is increased irt IR (60 min)
    *** cells: GM637 in BMS
    *** source: 15234984-Fig-3b
  *** Nbs1[Ab]IP phos(S343)[phosAb] is increased irt IR (15 min)
    *** cells: GM637 in BMS
    *** source: 16478990-Fig-4b
  *** xNbs1[phosAb] phos(S343)[phosAb] is increased irt IR (1 hr)
    *** cells: hFibros<Nbs1~fmut><xNbs1> in BMS
    *** source: 16327781-Fig-2c
  *** Nbs1[phosAb] phos(S343)[phosAb] is increased irt IR (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 10+ 15+ 30+ 60+ min
    *** source: 16327781-Fig-5b
  *** Nbs1[phosAb] phos(S343)[phosAb] is increased irt DSB-Induction (8 hr)
    *** cells: MCF7 in BMS
    *** source: 17486112-Fig-1b
  *** Nbs1[phosAb] phos(S343)[phosAb] is increased irt DSB-Induction (12 hr)
    *** cells: MCF7 in BMS "arrested in G1 by serum-depletion for 24 hr"
    *** source: 17486112-Fig-3a
  *** Nbs1[phosAb] phos(S343)[phosAb] is increased irt IR (tnr)
    *** cells: MCF7 in BMS
    *** source: 17486112-Fig-1b

=====================
Total Phosphorylation
=====================

reqs Atm--

  *** Nbs1[Ab]IP phos[WBMS-PPase] is increased irt IR (45 min)
    *** cells: hLymphoblasts<Atm~fmut><xAtm> in BMS
    *** reqs: xAtm [omission]
    *** source: 10766245-Fig-2d
  *** xNbs1[tAb]IP phos[32Pi] is increased irt IR (30 min)
    *** cells: HEK293T<xAtm><xNbs1> in BMS
    *** inhibited by: xAtr(D2870A/N2875K)"KD" [substitution]
    *** source: 10766245-Fig-3d
  *** Nbs1[Ab]IP phos[WBMS-PPase] is unchanged irt IR (45 min)
    *** cells: hLymphoblasts<Atm~fmut> in BMS
    *** source: 10766245-Fig-2b
  *** Nbs1[Ab]IP phos[WBMS-PPase] is unchanged irt IR (times)
    *** cells: GM1526<Atm~fmut> in BMS
    *** times: 0 1 2 4 6 hr
    *** source: 10766245-Fig-2c

not Prkdc--

  *** Nbs1[Ab]IP phos[WBMS-PPase] is increased irt IR (45 min)
    *** cells: hLymphoblasts<Prkdc~fmut> in BMS
    *** source: 10766245-Fig-2d


no extras

  *** Nbs1[Ab]IP phos[WBMS-PPase] is increased irt IR (45 min)
    *** cells: hLymphoblasts in BMS
    *** source: 10766245-Fig-2a
  *** Nbs1[Ab]IP phos[WBMS-PPase] is increased irt IR (45 min)
    *** cells: hLymphoblasts in BMS
    *** source: 10766245-Fig-2b
  *** Nbs1[Ab]IP phos[WBMS-PPase] is increased irt IR (times)
    *** cells: GM536 in BMS
    *** times: 0 1+ 2+ 4+ 6+ hr
    *** source: 10766245-Fig-2c
  *** xNbs1[tAb]IP phos[32Pi] is increased irt IR (30 min)
    *** cells: HEK293T<xAtm><xNbs1> in BMS
    *** inhibited by: xNbs1(S343A) [substitution]
    *** source: 10766245-Fig-3d

