===============
Ubiquitination
===============

Traf2--

  *** xRipk1[tAb]IP upshift[WBMS] is detectable
    *** cells: HEK293T in BMS "treated with MG132"
    *** reqs: Traf2 [RNAi]
    *** source: 15258597-Fig-3f
  *** xRipk1[tAb]IP ubiq[tUbAb] is increased itpo xTraf2
    *** cells: HEK293T<xUbiq(K63only)> in BMS "treated with MG132"
    *** inhibited by: xTnfaip3 [addition]
    *** source: 15258597-Fig-3e
  *** xRipk1[tAb]IP ubiq[tUbAb] is increased itpo xTraf2
    *** cells: HEK293T<xUbiq(K63only)> in BMS "treated with MG132"
    *** inhibited by: xUbiq(dig)"K48-only" [substitution]
    *** source: 15258597-Fig-3e

not Traf6--

  *** xRipk1[tAb]IP ubiq[tUbAb] is unchanged itpo xTraf6
    *** cells: HEK293T<xUbiq(K63only)> in BMS "treated with MG132"
    *** source: 15258597-Fig-3e

not Smad7--

  *** xRipk1[tAb]IP ubiq[tUbAb] is detectable
    *** cells: MCF7<xUbiq> in BMS
    *** unaffected by: xSmad7 [addition]
    *** source: 17384642-Fig-S6

Ubc13--

  *** xRipk1[tAb]IP upshift[WBMS] is detectable
    *** cells: HEK293T in BMS "treated with MG132"
    *** reqs: Ubc13 [RNAi]
    *** source: 15258597-Fig-3f

rUbc13 + rUev1a + rCiap2

  *** rRipk1[Ab] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbc13 + rUev1a + rCiap2)
    *** cells: none
    *** inhibited by: rUbiq(dig)"K48-only" [substitution]
    *** unaffected by: rUbiq(K63only) [substitution]
    *** source: 16547522-Fig-4b

rUbe2d1 + rTnfaip3

  *** rRipk1[tAb] ubiq[tUbAb] is increased by (rUbiq + rUbe1 + rUbe2d1 + rTnfaip3)
    *** cells: none
    *** source: 15258597-Fig-2c

rUbe2d1 + rCiap1

  *** rRipk1[Ab] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d1 + rCiap1)
    *** cells: none
    *** inhibited by: rCiap1(H588A) [substitution]
    *** source: 18621737-Fig-2d
  *** rRipk1[Ab] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d1 + rCiap1)
    *** cells: none
    *** unaffected by: rUbiq(dig)"K48-only" [substitution]
    *** unaffected by: rUbiq(K63only) [substitution]
    *** source: 18621737-Fig-2d
  *** Ripk1[Ab]IP upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d1 + rCiap1)
    *** cells: none
    *** IPfrom: mEFs<Ciap1~null> in BMS
    *** partially reqs: rCiap1 [omission]
    *** unaffected by: rUbiq(dig)"K48-only" [substitution]
    *** unaffected by: rUbiq(K63only) [substitution]
    *** source: 18621737-Fig-2c

rUbe2d3 + rCiap1

  *** xRipk1(D138N)[Ab]IP upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** partially inhibited by: rUbiq(dig)"no-lysines" [substitution]
    *** partially inhibited by: rUbiq(dig)"K48-only" [substitution]
    *** unaffected by: rUbiq(K48R) [substitution]
    *** partially inhibited by: rUbiq(K63only) [substitution]
    *** unaffected by: rUbiq(K63R) [substitution]
    *** source: 19854138-Fig-7e
  *** xRipk1(D138N)[Ab]IP upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** reqs: rUbe2d3 [omission]
    *** reqs: rCiap1 [omission]
    *** inhibited by: rUbe2d3(C85A) [substitution]
    *** source: 19854138-Fig-7b
  *** xRipk1(D138N)[Ab]IP upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** source: 19854138-Fig-7c
  *** xRipk1(D138N)[Ab]IP upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** source: 19854138-Fig-7d

rUbe2d3 + rTraf6

  *** xRipk1(D138N)[Ab]IP upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rTraf6)
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** source: 19854138-Fig-7d

not (rUbc13 + rUev1a) + rCiap1

  *** xRipk1(D138N)[Ab]IP upshift[WBMS] is unchanged by (rUbiq + rUbe1 + rUbc13 + rUev1a + rCiap1)
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** source: 19854138-Fig-7c

not rUbc13 + rCiap1

  *** rRipk1[Ab] upshift[WBMS] is unchanged by (rUbiq + rUbe1 + rUbc13 + rCiap1)
    *** cells: none
    *** source: 18621737-Fig-2d
  *** Ripk1[Ab]IP upshift[WBMS] is unchanged by (rUbiq + rUbe1 + rUbc13 + rCiap1)
    *** cells: none
    *** IPfrom: mEFs<Ciap1~null> in BMS
    *** source: 18621737-Fig-2c

not rUbe2d3 + rTraf2

  *** xRipk1(D138N)[Ab]IP upshift[WBMS] is unchanged by (rUbiq + rUbe1 + rUbe2d3 + rTraf2)
    *** cells: none
    *** IPfrom: SF9 in BMS
    *** source: 19854138-Fig-7d

================
Deubiquitination
================

not Cyld--

  *** xRipk1[tAb]IP ubiq[UbAb] is increased itpo xUbiq
    *** cells: HEK293T in BMS
    *** unaffected by: xCyld [addition]
    *** source: 19910467-Fig-3a
  *** xRipk1[tAb]IP upshift[WBMS] is increased itpo xUbiq
    *** cells: HEK293T in BMS
    *** unaffected by: xCyld [addition]
    *** source: 19910467-Fig-3a

Tnfaip3--

  *** xRipk1[tAb] upshift[WBMS] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xTnfaip3 [addition]
    *** source: 19910467-Fig-S1b
  *** xRipk1[tAb]IP upshift[WBMS] is decreased by rTnfaip3
    *** cells: HEK293T in BMS
    *** inhibited by: NEM [addition]
    *** source: 15258597-Fig-3d
  *** xRipk1[tAb]IP ubiq[tUbAb] is decreased itpo xTnfaip3
    *** cells: HEK293T<xUbiq(K63only)> in BMS "treated with MG132"
    *** inhibited by: xTnfaip3(C103A) [substitution]
    *** source: 15258597-Fig-4c
  *** xRipk1[tAb]IP ubiq[tUbAb] is decreased itpo xTnfaip3
    *** cells: HEK293T<xUbiq> in BMS "treated with MG132"
    *** inhibited by: xTnfaip3(C103A) [substitution]
    *** source: 15258597-Fig-3b
  *** xRipk1[tAb]IP ubiq[tUbAb] is increased itpo xTnfaip3
    *** cells: HEK293T<xUbiq(dig)"K48-only"> in BMS "treated with MG132"
    *** inhibited by: xTnfaip3(C103A) [substitution]
    *** source: 15258597-Fig-4c

Usp21--

  *** xRipk1[tAb]IP ubiq[tUbAb] is increased itpo xUbiq
    *** cells: HEK293T in BMS
    *** partially inhibited by: xUsp21 [addition]
    *** unaffected by: xUsp21(C221A)"CIA" [addition]
    *** source: 19910467-Fig-3a
  *** xRipk1[tAb] upshift[WBMS] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xUsp21 [addition]
    *** unaffected by: xUsp21(C221A)"CIA" [addition]
    *** source: 19910467-Fig-S1b
  *** xRipk1[tAb] upshift[WBMS] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xUsp21 [addition]
    *** unaffected by: xUsp21(C221A)"CIA" [addition]
    *** source: 19910467-Fig-S2

================================
Features of ubiquitinated Ripk1
================================

  *** xRipk1[tAb]IP ubiq[UbAb] is detectable
    *** cells: HEK293T<xCrmA> in BMS
    *** inhibited by: xRipk1(1-300) [substitution]
    *** unaffected by: xRipk1(301-550) [substitution]
    *** inhibited by: xRipk1(551-671) [substitution]
    *** source: 16543241-Fig-1c
  *** xRipk1[tAb]IP ubiq[UbAb] is detectable
    *** cells: HEK293T<xCrmA> in BMS
    *** inhibited by: xRipk1(K377R) [substitution]
    *** unaffected by: xRipk1(K396R) [substitution]
    *** unaffected by: xRipk1(K530R) [substitution]
    *** partially inhibited by: xRipk1(K305R/K306R) [substitution]
    *** source: 16543241-Fig-2c




================
coprecipitations
================

Csnk1a1--

  *** Ripk1[Ab] copptby[WB] Csnk1a1[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 18067272-Fig-3a
  *** xRipk1[Ab] copptby[WB] xNemo[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xTnfaip3 [addition]
    *** source: 10755617(D)

EgfR--

  *** xRipk1[tAb] copptby[WB] xEgfR[Ab] is detectable
    *** cells: HEK293EBNA in BMS
    *** source: 11116146-Fig-3b

Fadd--

  *** xRipk1[tAb] copptby[WB] xFadd[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 18439848-Fig-S9

Ikk1--  reqs Nemo

  *** xRipk1[tAb] copptby[WB] xIkk1[tAb] is detectable
    *** cells: HEK293T<xNemo> in BMS
    *** reqs: xNemo [omission]
    *** source: 10880512-Fig-5e

Ikk2--  reqs Nemo

  *** xRipk1[tAb] copptby[WB] xIkk2[tAb] is detectable
    *** cells: HEK293T<xNemo> in BMS
    *** reqs: xNemo [omission]
    *** source: 10880512-Fig-5e

Mavs--

  *** xRipk1[tAb] copptby[WB] xMavs[Ab] is undetectable
    *** cells: HEK293 in BMS
    *** source: 16125763-Fig-S5a
  *** xRipk1[tAb] copptby[WB] xMavs[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 16127453-Fig-5b
  *** xRipk1[tAb] copptby[WB] xMavs[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xTradd [addition]
    *** source: 18439848-Fig-4a
  *** xRipk1[tAb] copptby[WB] xMavs[tAb] is detectable
    *** cells: HEK293T<xFadd> in BMS
    *** source: 18439848-Fig-4d

not Mekk1--

  *** xRipk1[tAb] copptby[WB] xMekk1[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** source: 11429546-Fig-3h

Mekk3---

  *** xRipk1[tAb] copptby[WB] xMekk3[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 11429546-Fig-3h
  *** xMekk3[tAb] copptby[WB] xRipk1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xRipk1(D138N)"KD" [substitution]
    *** inhibited by: xRipk1(K45R)"KD" [substitution]
    *** source: 14585994-Fig-4d
  *** xRipk1[tAb] copptby[WB] xMekk3[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 15572679-Fig-1a

Nemo--

  *** rRipk1{ubiq}[Ab] boundby[WB] rNemo[GST] is detectable
    *** cells: none
    *** comment: rRipk1 was ubiquitinated with (rUbiq + rUbe1 + (rUbc13 + rUev1a) + rCiap2)
    *** inhibited by: rRipk1 [substitution]
    *** source: 16547522-Fig-4c

Sqstm1--

  *** rRipk1[35S] boundby[WB] rSqstm1[MalBP] is detectable
    *** cells: none
    *** source: 10356400-Fig-2

Trif--

  *** Ripk1[Ab] copptby[WB] xTrif[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 15064760-Fig-5a
  *** xRipk1[tAb] copptby[WB] xTrif[tAb] is detectable
    *** cells: HEK293T<xTradd> in BMS
    *** source: 18641654-Fig-6a
  *** xRipk1[tAb] copptby[WB] xTrif[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 14739303-Fig-7f

TLR3--

  *** xRipk1[tAb] copptby[WB] xTLR3[tAb] is detectable
    *** cells: HEK293<xTrif> in BMS
    *** reqs: xTrif [omission]
    *** source: 15064760-Fig-5a

Tnfaip3--

  *** Ripk1[Ab] copptby[WB] xTnfaip3[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 19910467-Fig-S1a

TnfR1--

  *** Ripk1[Ab] copptby[WB] xTnfR1[Ab] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xTnfaip3 [addition]
    *** source: 10755617-Fig-4e
  *** xRipk1[tAb] copptby[WB] xTnfR1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 8612133-Fig-1a
  *** xRipk1[tAb] copptby[WB] xTnfR1[Ab] is increased itpo xTradd
    *** cells: HEK293 in BMS
    *** source: 8612133-Fig-5a

not TnfR2--

  *** xRipk1[tAb] copptby[WB] xTnfR2[Ab] is undetectable
    *** cells: HEK293 in BMS
    *** source: 8612133-Fig-1a

Tnfaip3--

  *** Ripk1[Ab] copptby[WB] xTnfaip3[tAb] is detectable
    *** cells: HEK293T in BMS "treated with MG132"
    *** source: 15258597-Fig-2e
  *** Ripk1[Ab] copptby[WB] xTnfaip3[tAb] is detectable
    *** cells: HEK293T in BMS "treated with MG132"
    *** unaffected by: xTnfaip3(C103A) [substitution]
    *** source: 15258597-Fig-3c

Tradd--

  *** Ripk1[Ab] copptby[WB] xTradd[tAb] is detectable
    *** cells: HEK293T in BMS
    *** enhanced by: xRig1(1-200)"2Card-domain" [addition]
    *** source: 18439848-Fig-4c
  *** xRipk1[Ab] copptby[WB] xTradd[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xTrif [addition]
    *** source: 18641654-Fig-6a
  *** Ripk1[Ab] snaggedby[WB] rTradd[GST] is detectable
    *** cells: HELA in BMS "treated with Tnf (30 min)"
    *** inhibited by: rStat1 [addition]
    *** source: 10848577-Fig-4b
  *** xRipk1[tAb] copptby[WB] xTradd[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 8612133-Fig-1a
  *** xRipk1[tAb] copptby[WB] xTradd[Ab] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xRipk1(K377R)"Ubiq-deficient-mut" [substitution]
    *** source: 16603398-Fig-4b

Traf1--

  *** xRipk1[tAb] copptby[WB] xTraf1[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 8612133-Fig-1a

Traf2--

  *** xRipk1[tAb] copptby[WB] xTraf2[Ab] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xRipk1(K377R)"Ubiq-deficient-mut" [substitution]
    *** source: 16603398-Fig-4b
  *** xRipk1[tAb] copptby[WB] xTraf2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 8612133-Fig-1a
  *** xRipk1[tAb] copptby[WB] xTraf2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 8702708(D)
  *** xRipk1[tAb] copptby[WB] xTraf2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 10356400-Fig-3b,3c

Traf3--

  *** xRipk1[tAb] copptby[WB] xTraf3[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 8612133-Fig-1a

Trpc4ap--

  *** xRipk1[tAb] copptby[WB] xTrpc4ap[tAb] is detectable
    *** cells: COS7 in BMS
    *** source: 14585990-Fig-7b

Usp21--

  *** Ripk1[Ab] copptby[WB] xUsp21[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 19910467-Fig-2b
  *** Ripk1[Ab] copptby[WB] xUsp21[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 19910467-Fig-S1a
  *** xRipk1[tAb] copptby[WB] xUsp21[tAb] is detectable
    *** cells: HEK293T in BMS
    *** comment: coppt xRipk1 is mono-upshifted
    *** unaffected by: xUsp21(C221A)"CIA" [substitution]
    *** comment: xRipk1 coppt by xUsp21(C221A) has more upshifted bands
    *** source: 19910467-Fig-2c

Zfand5--

  *** Ripk1[Ab] copptby[WB] xZfand5[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 14754897-Fig-2d
  *** xRipk1[tAb] copptby[WB] xZfand5[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 14754897-Fig-2c









  *** xRipk1[tAb] prot-exp[WB] is decreased itpo xTnfaip3
    *** cells: HEK293T in BMS
    *** source: 15258597-Fig-2d
  *** xRipk1[tAb] prot-exp[WB] is decreased itpo xTnfaip3
    *** cells: HEK293T in BMS
    *** source: 15258597-Fig-4a
  *** xRipk1[tAb] prot-exp[WB] is decreased itpo xTnfaip3
    *** cells: HEK293T<xUbiq> in BMS
    *** inhibited by: xTnfaip3(C103A) [substitution]
    *** source: 15258597-Fig-4b

  *** xRipk1[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xRipk1(K45A)"KD" [substitution]
    *** source: 18067272-Fig-4a



