=============================================================
Direct Phosphorylation on T58 by Gsk3s requires Myc S62 site
=============================================================

  *** rMyc[phosAb] phos(T38)[phosAb] is increased by pGsk3s
    *** cells: none
    *** inhibited by: rMyc(S62A) [substitution]
    *** source: 15103331-Fig-S8

=====================================
Phosphophorylation on T58 ipto xGsk3s
=====================================

  *** xMyc[Ab]IP phos(T58)[phosAb] is increased itpo xGsk3a
    *** cells: COS7<xMyc> in BMS
    *** inhibited by: xMyc(T58A) [substitution]
    *** source: 14563837-Fig-2
  *** xMyc[Ab]IP phos(T58)[phosAb] is increased itpo xGsk3b
    *** cells: COS7<xMyc> in BMS
    *** inhibited by: xMyc(T58A) [substitution]
    *** source: 14563837-Fig-2

======================
Association with Gsk3s
======================

  *** rGsk3a[35S] boundby[WB] rMyc[Ab] is detectable
    *** cells: none
    *** source: 14563837-Fig-4c

  *** xGsk3b[tAb] copptby[WB] xMyc[Ab] is detectable
    *** cells: COS7<xGsk3b><xMyc> in BMS
    *** source: 14563837-Fig-4a
  *** xMyc[tAb] copptby[WB] xGsk3b[Ab] is detectable
    *** cells: COS7<xGsk3b><xMyc> in BMS
    *** source: 14563837-Fig-4b
  *** xMyc[tAb] copptby[WB] xGsk3a[Ab] is detectable
    *** cells: COS7<xGsk3a><xMyc> in BMS
    *** source: 14563837-Fig-4b
  *** xGsk3a[tAb] copptby[WB] xMyc[Ab] is detectable
    *** cells: COS7<xGsk3a><xMyc> in BMS
    *** source: 14563837-Fig-4a
  *** xMyc[tAb] copptby[WB] xGsk3a[Ab] is detectable
    *** cells: COS7<xGsk3a><xMyc> in BMS
    *** inhibited by: xMyc(101-439) [substitution]
    *** unaffected by: xMyc(del(101-143)) [substitution]
    *** partially inhibited by: xMyc(S62A) [substitution]
    *** partially inhibited by: xMyc(T58A) [substitution]
    *** source: 14563837-Fig-4d

==============
Colocalization
==============

  *** xGsk3a[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: COS7<xMyc><xGsk3a> in BMS
    *** comment: xGsk3a colocates with xMyc in the subnuclear-bodes
    *** source: 14563837-Fig-6a
  *** xGsk3b[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: COS7<xMyc><xGsk3b> in BMS
    *** comment: xGsk3b colocates with xMyc in the subnuclear-bodes
    *** source: 14563837-Fig-6b

==========
Relocation
==========

  *** xMyc[Ab] locatedin(subnuclear-bodies)[IHC] is increased itpo xGsk3a
    *** cells: COS7<xMyc> in BMS
    *** source: 14563837-Fig-5b
  *** xMyc[Ab] locatedin(subnuclear-bodies)[IHC] is increased itpo xGsk3b
    *** cells: COS7<xMyc> in BMS
    *** source: 14563837-Fig-5b

================================================
Myc Ubiquitination itpo xGsk3s requires T58 site
================================================

  *** xMyc[Ab]IP ubiq[UbAb] is increased itpo xGsk3a
    *** cells: COS7<xMyc> in BMS
    *** inhibited by: xMyc(T58A) [substitution]
    *** source: 14563837-Fig-2
  *** xMyc[Ab]IP upshift[WBMS] is increased itpo xGsk3a
    *** cells: COS7<xMyc> in BMS
    *** inhibited by: xMyc(T58A) [substitution]
    *** source: 14563837-Fig-2
  *** xMyc[Ab]IP ubiq[UbAb] is increased itpo xGsk3b
    *** cells: COS7<xMyc> in BMS
    *** inhibited by: xMyc(T58A) [substitution]
    *** source: 14563837-Fig-2
  *** xMyc[Ab]IP upshift[WBMS] is increased itpo xGsk3b
    *** cells: COS7<xMyc> in BMS
    *** inhibited by: xMyc(T58A) [substitution]
    *** source: 14563837-Fig-2


