=====================================
Proteins required for mRNA expression
=====================================

Irak1-- ???

  *** IL8-gene mRNA[Northern] is increased irt IL1b (6 hr)
    *** cells: HEK293 in BMS
    *** reqs:  Irak1 [null-mut]
    *** source: 10373513-Fig-3c
  *** IL8-gene mRNA[RTPCR] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** does not req: Irak1 [RNAi]
    *** source: 19181383-Fig-3b

Irak4--

  *** IL8-gene mRNA[RTPCR] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** reqs: Irak1 and/or Irak4 [RNAi]
    *** source: 19181383-Fig-3b
  *** IL8-gene mRNA[RTPCR] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** reqs: Irak4 [RNAi]
    *** comment: response can be rescued with either xIrak4 or xIrak4(K213A/K214A)"KD"
    *** comment: response is not rescued with xIrak4(1-185)
    *** source: 19181383-Fig-4a
  *** IL8-gene mRNA[RTPCR] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** reqs: Irak4 [RNAi]
    *** source: 19181383-Fig-3a
  *** IL8-gene mRNA[Northern] is increased irt IL1b
    *** cells: hFibros<Irak4~null><xIrak4> in BMS
    *** times: 0 1+ 2+ 3+ 4+ hr
    *** reqs: xIrak4 [omission]
    *** unaffected by: xIrak4(K213A/K214A) [substitution]
    *** source: 15084582-Fig-4

Myd88--

  *** IL8-gene mRNA[Northern] is increased irt IL1b (6 hr)
    *** cells: HEK293 in BMS
    *** partially reqs: Myd88 [null-mut]
    *** source: 10373513-Fig-3c

Peli1--

  *** IL8-gene mRNA[Northern] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 4+ 8+ 12+ 24+ hr
    *** partially reqs: Peli1 [RNAi]
    *** source: 12496252-Fig-3c

Peli2--

  *** IL8-gene promo-reporter[Luc] is increased irt IL1b (5 hr)
    *** cells: C3H10T12 in BMS
    *** reqs: Peli2 [AS-DNA]
    *** source: 12370331-Fig-3c

Tab1--

  *** IL8-gene promo-reporter[Luc] is increased irt IL1a (5 hr)
    *** cells: HELA in BMS
    *** inhibited by: xTab1(1-418) [addition]
    *** source: 11050078-Fig-7b
  *** IL8-gene promo-reporter[Luc] is increased irt IL1a (5 hr)
    *** cells: HELA in BMS
    *** unaffected by: xTab1 [addition]
    *** source: 11050078-Fig-7c

Tak1--

  *** IL8-gene promo-reporter[Luc] is increased irt IL1a (5 hr)
    *** cells: HELA in BMS
    *** inhibited by: xTak1(K63W) [addition]
    *** inhibited by: xTak1(1-77) [addition]
    *** source: 11050078-Fig-7b
  *** IL8-gene promo-reporter[Luc] is increased irt IL1a (5 hr)
    *** cells: HELA in BMS
    *** inhibited by: xTak1(K63W) [addition]
    *** source: 11050078-Fig-6a
  *** IL8-gene promo-reporter[Luc] is increased irt IL1a (5 hr)
    *** cells: HELA in BMS
    *** unaffected by: xTak1 [addition]
    *** inhibited by: xTak1(1-77) [addition]
    *** source: 11050078-Fig-7b

Tirap--  ????

  *** IL8-gene promo-reporter[Luc] is increased irt IL1 (6 hr)
    *** cells: HEK293 in BMS
    *** inhibited by: xTirap(D198A)"cleavage-site-mut" [addition]
    *** source: 17360653(D)

Other--

  *** IL8-gene promo-reporter[Luc] is increased irt IL1a (5 hr)
    *** cells: HELA in BMS
    *** comment: inhibited by removal of Ap1 binding site on IL8-gene promo-reporter
    *** comment: inhibited by removal of Nfkb binding site on IL8-gene promo-reporter
    *** source: 11050078-Fig-3b

=========
no extras
=========

  *** IL8-gene mRNA[RTPCR] is increased irt IL1 (times)
    *** cells: HELA in BMS
    *** times: 0 1+++ 2++ 4++ 6+ 9+ 12+ 16+ hr
    *** source: 16371247-Fig-2d
  *** IL8-gene mRNA[RTPCR] is increased irt IL1b (times)
    *** cells: hKerats in BMS
    *** times: 0 2++ 6+ 18+ hr
    *** source: 15308104-Fig-7b
  *** IL8-gene mRNA[RPA] is increased irt IL1 (3 hr)
    *** cells: hHepatocytes in BMS
    *** source: 15123776-Fig-2


====================================
Proteins required for IL1b secretion
====================================

not Irak1--

  *** IL8[Ab] secretion[ELISA] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** does not req: Irak1 [RNAi]
    *** source: 19181383-Fig-2a

Irak4--

  *** IL8[Ab] secretion[ELISA] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** reqs: Irak4 [RNAi]
    *** source: 19181383-Fig-2a

Tak1--

  *** IL8[Ab] secretion[ELISA] is increased irt IL1a (17 hr)
    *** cells: HELA in BMS
    *** unaffected by: xTak1 [addition]
    *** inhibited by: xTak1(1-77) [addition]
    *** source: 11050078-Fig-8b

