==============
Stat3 to EgfR
==============

  *** Stat3[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** source: 14963038-Fig-7a
  *** xEgfR[Ab] copptby[WB] Stat3[Ab] is increased irt Egf (15 min)
    *** cells: hESKs<xEgfR> in BMLS "Egf and BPE starved"
    *** source: 15284024-Fig-5a
  *** Stat3[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0+ 5+ 15++ 30+ 60+ 120+ min
    *** source: 10358079-Fig-3b

Coppt in cells with "hyperactive Stat3,EgfR,Src"

  *** Stat3[Ab] copptby[WB] EgfR[Ab] is detectable
    *** cells: COLO357 in BMS
    *** source: 21573184-Fig-1a
  *** Stat3[Ab] copptby[WB] EgfR[Ab] is detectable
    *** cells: PANC1 in BMS
    *** does not req: Src [RNAi]
    *** source: 21573184-Fig-1c
  *** Stat3[Ab] copptby[WB] EgfR[Ab] is detectable
    *** cells: PANC1 in BMS
    *** source: 21573184-Fig-1a
  *** Stat3[Ab] copptby[WB] EgfR[Ab] is undetectable
    *** cells: A549 in BMS
    *** source: 21573184-Fig-S1c
  *** Stat3[Ab] copptby[WB] EgfR[Ab] is undetectable
    *** cells: MDAMB231 in BMS
    *** source: 21573184-Fig-S1c
  *** EgfR[Ab] copptby[WB] Stat3[Ab] is detectable
    *** cells: COLO357 in BMS
    *** source: 21573184-Fig-1a
  *** EgfR[Ab] copptby[WB] Stat3[Ab] is detectable
    *** cells: PANC1 in BMS
    *** does not req: Src [RNAi]
    *** source: 21573184-Fig-1c
  *** EgfR[Ab] copptby[WB] Stat3[Ab] is detectable
    *** cells: PANC1 in BMS
    *** source: 21573184-Fig-1a

No Coppt in other cells

  *** EgfR[Ab] copptby[WB] Stat3[Ab] is undetectable
    *** cells: A549 in BMS
    *** source: 21573184-Fig-S1c
  *** EgfR[Ab] copptby[WB] Stat3[Ab] is undetectable
    *** cells: MDAMB231 in BMS
    *** source: 21573184-Fig-S1c


=====
Yphos
=====

Mekk1--

  *** Stat3[Ab]IP Yphos[pYAb] is increased irt Egf (5 min)
    *** cells: HELA in BMS
    *** partially inhibited by: xMekk1(K1255M)"DN" [addition]
    *** source: 11278353-Fig-1b

Chemicals

  *** Stat3[Ab]IP Yphos[pYAb] is increased irt Egf (10 min)
    *** cells: A431 in BMLS
    *** inhibited by: PP1 [addition]
    *** inhibited by: CGP77675 [addition]
    *** source: 10358079-Fig-6b

No extras

  *** Stat3[Ab]IP Yphos[pYAb] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 5++ 15+ 30+ 60+ 120 min
    *** source: 10358079-Fig-2b
  *** xStat3[tAb]IP Yphos[pYAb] is increased irt Egf (15 min)
    *** cells: COS7 in BMS
    *** source: 9083098-Fig-1
  *** Stat3[Ab]IP Yphos[pYAb] is increased irt Egf (15 min)
    *** cells: A431 in BMLS
    *** source: 8140422-Fig-2a

==========
phos(Y705)
==========

Amph--  ???

  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (times)
    *** cells: NIH3T3<xEgfR> in BMS
    *** times: 0 10+ 30+ min
    *** unaffected by: xAmph [addition]
    *** source: 12093727-Fig-3a

EgfR-- chemicals only

  *** Stat3[phosAb] phos(Y1069)[phosAb] is increased irt Egf (30 min)
    *** cells: MDAMB468 in BMLS
    *** bkg inhibited by: Gefitinib [pretreatment]
    *** source: 15950906-Fig-5c

Epn2--  ???

  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (times)
    *** cells: NIH3T3<xEgfR> in BMS
    *** times: 0 10+ 30+ min
    *** unaffected by: xEpn2 [addition]
    *** source: 12093727-Fig-3a

Fak2--

  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** partially inhibited by: xFak2(K457A/D567A)"DN" [addition]
    *** source: 14963038-Fig-6b

Grb2--

  *** xStat3[phosAb] phos(S705)[phosAb] is increased irt Egf (15 min)
    *** cells: COS1 in BMS
    *** inhibited by: xGrb2 [addition]
    *** source: 14498832-Fig-1a

Hes1--

  *** xStat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (times)
    *** cells: COS1 in BMS
    *** times: 0 5+ 30++ min
    *** bkg reqs: Hes1 [RNAi]
    *** source: 15156153-Fig-2c

Jak1-- Jak2--  chemicals only


  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (30 min)
    *** cells: MDAMB468 in BMLS
    *** partially inhibited by: AG490 [pretreatment] no-bkg-control
    *** source: 15950906-Fig-5a
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (30 min)
    *** cells: MDAMB468 in BMLS
    *** partially inhibited by: AG490 [pretreatment] no-bkg-control
    *** source: 15950906-Fig-S3

Mekk1--

  *** xStat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (15 min)
    *** cells: COS1 in BMS
    *** inhibited by: xMekk1(K1255M)"DN" [addition]
    *** unaffected by: xMekk1(CatDomain)"CA" [addition]
    *** source: 11278353-Fig-1a

Src--

  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** inhibited by: xSrc(K297R/Y530F)"DN" [addition]
    *** source: 14963038-Fig-6b,7b

Socs1-- Socs3--  ???

  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (6 hr)
    *** cells: NIH3T3<xEgfR> in BMS
    *** unaffected by: xSocs1 [addition]
    *** unaffected by: xSocs3 [addition]
    *** source: 12070153-Fig-6b

not Stat1--

  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (10 min)
    *** cells: A431 in BMS
    *** does not req: Stat1 [RNAi]
    *** source: 17646016-Fig-4

Chemicals

  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (times)
    *** cells: NIH3T3<xEgfR> in BMS
    *** times: 0 10+ 30+ min
    *** unaffected by: PAO [addition]
    *** source: 12093727-Fig-3a
  *** xStat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (15 min)
    *** cells: COS1 in BMS
    *** inhibited by: AG1478 [addition]
    *** source: 11278353-Fig-1a
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (10 min)
    *** cells: A431 in BMS
    *** inhibited by: AG1478 [addition]
    *** inhibited by: Compound-56 [addition]
    *** partially inhibited by: CGP77675 [addition]
    *** source: 17646016-Fig-3b
  ---------------------------------------------------------------------
  *** Stat3[phosAb] phos(Y705)[phosAb] is detectable
    *** cells: MDAMB468 in BMLS "treated with Egf for 5 hr"
    *** inhibited by: Gefitinib [pretreatment]
    *** inhibited by: AG1478 [pretreatment]
    *** unaffected by: AG490 [pretreatment]
    *** source: 17909010-Fig-3a

Other

  *** xStat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (6 hr)
    *** cells: COS1<xStat3> in BMS
    *** unaffected by: xMkk7 [addition]
    *** unaffected by: xJnk1 [addition]
    *** unaffected by: xJnk1(mnr)"KD" [addition]
    *** inhibited by: xMkk7 + xJnk1 [addition]
    *** unaffected by: xMkk7 +  xJnk1(mnr)"KD" [addition]
    *** unaffected by: xMkk7(mnr)"KD" +  xJnk1 [addition]
    *** source: 10521505-Fig-5c

No extras

  *** xStat3[tAb]IP phos(Y705)[phosAb] is increased irt Egf (30 min)
    *** cells: HEK293 in BMLS
    *** source: 9405487(D)
  *** Stat3[phosAb] phos(Y705)[phosAb] is unchanged irt Egf (30 min)
    *** cells: NIH3T3<xEgfR> in BMS
    *** source: 12070153-Fig-2f
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (5 min)
    *** cells: mSECs in BMLS
    *** source: 15059889-Fig-3a
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (10 min)
    *** cells: A431 in BMS
    *** source: 17646016-Fig-1b
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (10 min)
    *** cells: A4318a in BMS
    *** source: 17646016-Fig-1b
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (1 hr)
    *** cells: HN5 in BMS
    *** source: 17918184-Fig-3a
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (times)
    *** cells: A431 in BMS
    *** times: 0 1++ 2++ 4++ 8++ 16+ 24+ hr
    *** source: 17918184-Fig-2a
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (times)
    *** cells: HN5 in BMS
    *** times: 0 1++ 2++ 4++ 8++ 16+ 24+ hr
    *** source: 17918184-Fig-2a
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (5 min)
    *** cells: HEK293T in BMS
    *** source: 11598209-Fig-3a
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** source: 10446219-Fig-2b
  *** xStat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (15 min)
    *** cells: COS1<xStat3> in BMS
    *** source: 10521505-Fig-1a
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (times)
    *** cells: MDAMB468 in BMLS
    *** times: 0 1+ 2+ 4+ hr
    *** source: 17909010-Fig-3b
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt Egf (times)
    *** cells: MDCK in BMLS
    *** times: 0 1+ 2++ 4++ 6++ 24++ hr
    *** source: 17909010-Fig-3c

==========
phos(S727)
==========

Fak2--

  *** Stat3[phosAb] phos(S727)[phosAb] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** partially inhibited by: xFak2(K457A/D567A)"DN" [addition]
    *** source: 14963038-Fig-6b

Grb2--  ???

  *** xStat3[phosAb] phos(S727)[phosAb] is increased irt Egf (15 min)
    *** cells: COS1 in BMS
    *** unaffected by: xGrb2 [addition]
    *** source: 14498832-Fig-1a

Mekk1--

  *** xStat3[phosAb] phos(S727)[phosAb] is increased irt Egf (15 min)
    *** cells: COS1 in BMS
    *** partially inhibited by: xMekk1(K1255M)"DN" [addition]
    *** unaffected by: xMekk1(CatDomain)"CA" [addition]
    *** source: 11278353-Fig-1a

Src--

  *** Stat3[phosAb] phos(S727)[phosAb] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** inhibited by: xSrc(K297R/Y530F)"DN" [addition]
    *** source: 14963038-Fig-6b,7b

not Stat1--

  *** Stat3[phosAb] phos(Y727)[phosAb] is increased irt Egf (10 min)
    *** cells: A431 in BMS
    *** does not req: Stat1 [RNAi]
    *** source: 17646016-Fig-4

Chemicals

  *** xStat3[phosAb] phos(S727)[phosAb] is increased irt Egf (15 min)
    *** cells: COS1<xStat3> in BMS
    *** unaffected by: SB203580 [pretreatment]
    *** bkg inhibited by: PD98059 [pretreatment]
    *** source: 10521505-Fig-4

No extras

  *** Stat3[phosAb] phos(S727)[phosAb] is increased irt Egf (5 min)
    *** cells: HEK293T in BMLS
    *** source: 11598209-Fig-6
  *** xStat3[phosAb] phos(S727)[phosAb] is increased irt Egf (15 min)
    *** cells: COS1<xStat3> in BMS
    *** source: 10521505-Fig-1a

=====
Sphos
=====

  *** Stat3[Ab]IP Sphos[pSAb] is unchanged irt Egf (5 min)
    *** cells: HELA in BMS
    *** source: 11278353-Fig-1b

=====================
Total Phosphorylation
=====================

  *** xStat3[Ab]IP phos[32Pi] is increased irt Egf (15 min)
    *** cells: COS1<xStat3> in BMS
    *** source: 10521505-Fig-2c

============
reporter-act
============

  *** Stat3-reporter[Luc] is increased irt Egf (6 hr)
    *** cells: HELA<xStat3> in BMS
    *** source: 14963038-Fig-6a

===========
DNA-binding
===========

  *** Stat3[RE] oligo-binding[EMSA] is increased irt Egf (times)
    *** cells: NIH3T3<xEgfR> in BMS
    *** times: 0+ 10++ 30+++ min
    *** source: 12093727-Fig-2a
  *** Stat3[Ab] snaggedby[WB] oligo[biotin] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** source: 17918184-Fig-2b
  *** Stat3[Ab] snaggedby[WB] oligo[biotin] is increased irt Egf (15 min)
    *** cells: HN5 in BMS
    *** source: 17918184-Fig-2b
  *** Stat3[Ab] oligo-binding[EMSA] is increased irt Egf (15 min)
    *** cells: A431 in BMLS
    *** source: 8140422-Fig-3b
  *** xStat3[Ab] oligo-binding[EMSA] is increased irt Egf (15 min)
    *** cells: COS1 in BMLS "nuclear-extracts"
    *** source: 8140422-Fig-4

======================
Other Coprecipitations
======================

Fak2--

  *** Stat3[Ab] copptby[WB] Fak2[Ab] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** source: 14963038-Fig-7a

Jak1--

  *** Stat3[Ab] copptby[WB] Jak1[Ab] is increased irt Egf (15 min)
    *** cells: hESKs<xEgfR> in BMLS "Egf and BPE starved"
    *** source: 15284024-Fig-5b

Jak2--

  *** Stat3[Ab] copptby[WB] Jak2[Ab] is increased irt Egf (15 min)
    *** cells: hESKs<xEgfR> in BMLS "Egf and BPE starved"
    *** source: 15284024-Fig-5b
  *** Stat3[Ab] copptby[WB] Jak2[Ab] is increased irt Egf (15 min)
    *** cells: hESKs in BMLS "Egf and BPE starved"
    *** source: 15284024-Fig-5b

Pkcd--

  *** Stat3[Ab] copptby[WB] Pkcd[Ab] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** source: 10446219-Fig-2a

Src--

  *** Stat3[Ab] copptby[WB] Src[Ab] is increased irt Egf (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: xRala(S28N)"DN" [addition]
    *** source: 10675331-Fig-4a

Stat1--

  *** Stat3[Ab] copptby[WB] Stat1[Ab] is increased irt Egf (15 min)
    *** cells: hESKs<xEgfR> in BMLS "Egf and BPE starved"
    *** source: 15284024-Fig-5a
  *** Stat3[Ab] copptby[WB] Stat1[Ab] is increased irt Egf (15 min)
    *** cells: hESKs in BMLS "Egf and BPE starved"
    *** source: 15284024-Fig-5a

