==========================
H2ax-Mdc1 binding requires
==========================

H2ax phos(S140)

  *** Mdc1[Ab] snaggedby[WB] sH2ax(124-143){phos(S140)}[biotin] is detectable
    *** cells: HELA in BMS
    *** inhibited by: sH2ax(124-143) [substitution]
    *** source: 15201865-Fig-5b
  *** Mdc1[Ab] snaggedby[WB] sH2ax(134-143){phos(S140)}[biotin] is detectable
    *** cells: HELA in BMS "nuclear-extract"
    *** inhibited by: sH2ax(134-143) [substitution]
    *** source: 18583988-Fig-1c

Mdc1 BRACT domain (1894-2089)


  *** rMdc1(1894-2089)"BRCT"[Ab] boundby[WB] sH2ax(134-143){phos(S140)}[Sepharose] is detectable
    *** cells: none
    *** partially inhibited by: sH2ax(134-143) [substitution]
    *** source: 16427009-Fig-5a
  *** xMdc1[tAb] snaggedby[WB] sH2ax(134-143){phos(S140)}[Sepharose] is detectable
    *** cells: HEK293T<xMdc1> in BMS
    *** inhibited by: xMdc1(del(1894-2089))"delBRCT" [substitution]
    *** source: 16427009-Fig-5b
  *** rMdc1(56-124)"FHA"[Ab] boundby[WB] sH2ax(134-143){phos(S140)}[Sepharose] is undetectable
    *** cells: none
    *** unaffected by: sH2ax(134-143) [substitution]
    *** source: 16427009-Fig-5a



  *** rMdc1[Ab] boundby[WB] sH2ax(134-143){phos(S140)}[Sepharose] is detectable
    *** cells: none
    *** unaffected by: xAtm[Ab]IP [addition]
    *** source: 16427009-Fig-4g
  *** Mdc1[Ab] snaggedby[WB] sH2ax(134-143){phos(S140)}[biotin] is detectable
    *** cells: HELA in BMS "nuclear-extract"
    *** source: 18583988-Fig-2a


