========
Shedding
========

Adam17--  phos(T735)

  *** xTgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** reqs: Adam17 [RNAi]
    *** source: 20188673-Fig-3b
  *** Tgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: T42 in BMLS
    *** reqs: Adam17 [RNAi]
    *** source: 20188673-Fig-3c
  *** xTgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: HELA<xProTgfa> in BMLS
    *** reqs: Adam17 [RNAi]
    *** source: 20188673-Fig-S2b
  *** xTgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: M2<Adam17~fmut><xProTgfa><xAdam17> in BMLS
    *** unaffected by: xAdam17(S819A) [substitution]
    *** unaffected by: xAdam17(S791A) [substitution]
    *** inhibited by: xAdam17(T735A) [substitution]
    *** source: 20188673-Fig-5b

P38a--

  *** xTgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** reqs: P38a [RNAi]
    *** source: 20188673-Fig-2c
  *** xTgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: M2<Adam17~fmut><xProTgfa><xAdam17> in BMLS
    *** inhibited by: SB203580 [cotreatment]
    *** inhibited by: xP38a(T180A/Y182F) [addition]
    *** source: 20188673-Fig-5b
  *** xTgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: HELA<xProTgfa> in BMLS
    *** reqs: P38a [RNAi]
    *** source: 20188673-Fig-S2a

chemicals

  *** xTgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: M2<Adam17~fmut><xAdam17><xProTgfa> in BMS
    *** inhibited by: SB203580 [pretreatment]
    *** source: 22550340-Fig-5a
  *** xTgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: HELA<xProTgfa> in BMLS
    *** inhibited by: SB203580 [pretreatment]
    *** source: 20188673-Fig-S1b
  *** Tgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: T42 in BMLS
    *** inhibited by: SB203580 [pretreatment]
    *** source: 20188673-Fig-1e

no extras

  *** Tgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: T42 in BMS
    *** source: 22550340-Fig-6d
  *** xTgfa[Ab] secretion[ELISA] is increased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** source: 20188673-Fig-1c

===============================================================
Decrease in Total Protein - due to shedding of surface Tgfa???
===============================================================

Adam17--

  *** xProTgfa[Ab] prot-exp[WB] is decreased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** comment: all bands are gone
    *** reqs: Adam17 [RNAi]
    *** comment: removal of Adam17 does not restore two upper bands
    *** source: 20188673-Fig-3a

P38a--

  *** xProTgfa[Ab] prot-exp[WB] is decreased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** comment: all bands are gone
    *** reqs: P38a [RNAi]
    *** source: 20188673-Fig-2b



chemicals

  *** xProTgfa[Ab] prot-exp[WB] is decreased irt Anisomycin (45 min)
    *** cells: HELA<xProTgfa> in BMLS
    *** reversed by: SB203580 [pretreatment]
    *** source: 20188673-Fig-S1a
  *** xProTgfa[Ab] prot-exp[WB] is decreased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** comment: all bands are gone
    *** partially reversed by: CHX [pretreatment]
    *** source: 20188673-Fig-2d
  *** xProTgfa[Ab] prot-exp[WB] is decreased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** comment: all bands are gone
    *** reversed by: SB203580 [pretreatment]
    *** source: 20188673-Fig-2a
  *** xProTgfa[Ab] prot-exp[WB] is decreased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** reversed by: SB203580 [pretreatment]
    *** reversed by: TAPI1 [pretreatment]
    *** source: 20188673-Fig-6a
  *** xProTgfa[Ab] prot-exp[WB] is decreased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** comment: all bands are gone
    *** unaffected by: U0126 [pretreatment]
    *** reversed by: SB203580 [pretreatment]
    *** unaffected by: JNK-Inhibitor-II [pretreatment]
    *** unaffected by: Bisindolylmaleimide-I [pretreatment]
    *** source: 20188673-Fig-1d

no extras

  *** xProTgfa[Ab] prot-exp[WB] is decreased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** comment: all bands are gone
    *** source: 20188673-Fig-1b
  *** xProTgfa[Ab] prot-exp[WB] is decreased irt Anisomycin (60 min)
    *** cells: CHO<xProTgfa> in BMLS
    *** source: 20188673-Fig-5c


