========================
Phosphorylation on S2152
========================

reqs Rsks--

  *** xFlna[phosAb] phos(S2152)[phosAb] is increased irt Egf (15 min)
    *** cells: HEK293E<xFlna> in BMLS
    *** enhanced by: xRsk!Chick[tAb]IP [addition]
    *** inhibited by: xRsk!Chick(K112R/K464R)[tAb]IP [addition]
    *** source: 15024089-Fig-6b
  *** Flna[phosAb] phos(S2152)[phosAb] is increased irt Egf (15 min)
    *** cells: HELA in BMLS
    *** partially reqs: Rsk1 [RNAi]
    *** partially reqs: Rsk2 [RNAi]
    *** partially reqs: Rsk1 and/or Rsk2 [RNAi]
    *** source: 15024089-Fig-7

Chemicals

  *** Flna[phosAb] phos(S2152)[phosAb] is increased irt Egf (15 min)
    *** cells: 3Y1 in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** source: 15024089-Fig-4a
  *** Flna[phosAb] phos(S2152)[phosAb] is increased irt Egf (15 min)
    *** cells: 3Y1 in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** unaffected by: H89 [pretreatment] no-bkg-control
    *** unaffected by: Wortmannin [pretreatment] no-bkg-control
    *** source: 15024089-Fig-5b
  *** Flna[phosAb] phos(S2152)[phosAb] is increased irt Egf (15 min)
    *** cells: HEK293E in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** source: 15024089-Fig-4a

No extras

  *** Flna[phosAb] phos(S2152)[phosAb] is increased irt Egf (15 min)
    *** cells: 3Y1 in BMLS
    *** source: 15024089-Fig-5a
  *** xFlna[tAb]IP phos(S2152)[phosAb] is increased irt Egf (15 min)
    *** cells: HEK293E<xFlna> in BMLS
    *** inhibited by: xFlna(S2152A) [substitution]
    *** source: 15024089-Fig-4c

=====================
Total Phosphorylation
=====================

  *** xFlna[tAb]IP phos[32Pi] is increased irt Egf (10 min)
    *** cells: HEK293E<xFlna> in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** source: 15024089-Fig-3b
  *** xFlna[tAb]IP phos[32Pi] is increased irt Egf (15 min)
    *** cells: HEK293E<xFlna> in BMLS
    *** enhanced by: xRsk!Chick[tAb]IP [addition]
    *** inhibited by: xRsk!Chick(K112R/K464R)[tAb]IP [addition]
    *** source: 15024089-Fig-6a
