===============
Phosphorylation
===============

autophos on T294 requires S526 phos site

  *** Mekk3[Ab]IP phos(T294)[phosAb] is detectable
    *** cells: RAW2647 in BMS
    *** source: 18308725-Fig-5b
  *** xMekk3[phosAb] phos(T294)[phosAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** source: 18308725-Fig-2d
  *** xMekk3[tAb]IP phos(T294)[phosAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** source: 18308725-Fig-4a

  *** xMekk3[tAb]IP phos(T294)[phosAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMekk3(S526A) [substitution]
    *** partially inhibited by: xMekk3(S526D) [substitution]
    *** source: 18308725-Fig-4a

autophos on S526 does not req T294 or T530 phos sites

  *** xMekk3[phosAb] phos(S526)[phosAb] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMekk3(T294A) [substitution]
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** source: 18308725-Fig-4a
  *** xMekk3[phosAb] phos(S526)[phosAb] is detectable
    *** cells: HEK293EBNA in BMS
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** inhibited by: xMekk3(S526A) [substitution]
    *** unaffected by: xMekk3(T530A) [substitution]
    *** source: 16407301(D)
  *** xMekk3[tAb] phos[WBMS] is detectable
    *** cells: HEK293EBNA in BMS
    *** inhibited by: xMekk3(S526A) [substitution]
    *** source: 16407301(D)

phos(T530)

  *** xMekk3[tAb] phos[WBMS] is detectable
    *** cells: HEK293EBNA in BMS
    *** inhibited by: xMekk3(T530A) [substitution]
    *** source: 16407301(D)

phosphorylation of expressed Mekk3 requires Mekk3 kinase activity

  *** xMekk3[tAb] phos[WBMS] is detectable
    *** cells: HEK293EBNA in BMS
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** unaffected by: xMekk3(T516A) [substitution]
    *** unaffected by: xMekk3(T528A) [substitution]
    *** unaffected by: xMekk3(T511A) [substitution]
    *** unaffected by: xMekk3(T520A) [substitution]
    *** unaffected by: xMekk3(T522A) [substitution]
    *** source: 16407301(D)
  *** xMekk3[tAb] phos[WBMS-PPase] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xMekk3(S526A/K391M) [substitution]
    *** source: 18206350-Fig-3b

================================
Expressed Mekk3 is kinase active
================================

  *** xMekk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMekk3(T294A) [substitution]
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** source: 18308725-Fig-7a
  *** xMekk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** source: 15572679-Fig-9

===============
Oligomerization
===============

  *** xMekk3[tAb] oligomerization[2TagPpt] is detectable
    *** cells: HEK293T in BMS
    *** source: 19843958-Fig-6c

================
Coprecipitations
================

Gadd45b--

  *** xMekk3[tAb] copptby[WB] xGadd45b[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** source: 14743220-Fig-1a

Ikbb--

  *** Mekk3[Ab] copptby[WB] Ikbb[Ab] is undetectable
    *** cells: mEFs in BMS "on gelatin"
    *** unaffected by: IL1a (5 min) [stim]
    *** unaffected by: Tnf (5 min) [stim]
    *** source: 14636585-Fig-6f

Ikk2--

  *** xMekk3[tAb] copptby[WB] xIkk2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMekk3(K391A)"KD" [substitution]
    *** source: 15572679-Fig-8a

Nemo--

  *** xMekk3[tAb] copptby[WB] xNemo[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMekk3(K395A)"KD" [substitution]
    *** source: 15572679-Fig-8a

Ripk--

  *** xMekk3[tAb] copptby[WB] Ripk1[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 14585994-Fig-4a
  *** xMekk3[tAb] copptby[WB] xRipk1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 14585994-Fig-4c

Sqstm1--

  *** xMekk3[tAb]IP copptby[WB] xSqstm1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xMekk3(K48A) [substitution]
    *** source: 19843958-Fig-S17

Tak1--

  *** xMekk3[tAb] copptby[WB] xTak1[Ab] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xMekk3(S526A/K391M) [substitution]
    *** source: 18206350-Fig-6a
  *** xMekk3[tAb] copptby[WB] xTak1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 19843958-Fig-S4

Traf6--

  *** xMekk3[tAb] copptby[WB] xTraf6[tAb] is detectable
    *** cells: mEFs in BMS
    *** source: 14661019(D)

Ywhas--  reqs Mekk3 kinase activity  Mekk3 T294 S526 T530

  *** Ywhas[Ab] copptby[WB] xMekk3[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMekk3(T294A) [substitution]
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** inhibited by: xMekk3(S526A)"activation-loop-mut" [substitution]
    *** unaffected by: xMekk3(S357A) [substitution]
    *** source: 18308725-Fig-1a,3a
  *** Ywhas[Ab] copptby[WB] xMekk3[tAb] is detectable
    *** cells: HEK293EBNA in BMS
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** inhibited by: xMekk3(S526A) [substitution]
    *** inhibited by: xMekk3(T530A) [substitution]
    *** source: 16407301(D)






















