=================================================
Binding to Ywhaq decreases Pdpk1 kinase activity
=================================================

  *** rPdpk1[32P] IVKA(auto)[32P-ATP] is detectable
    *** cells: none
    *** comment: Rsk2 peptide was added to reaction
    *** comment: IVKA was enhanced if Rsk2 peptide was phosed on S386
    *** inhibited by: rYwhaq [addition]
    *** source: 12177059-Fig-5a
  *** xPdpk1[tAb]IP IVKA(Sgk1)[32P-ATP] is decreased itpo xYwhaq
    *** cells: COS7<xPdpk1> in BMS
    *** comment: Rsk2 phos(S386)peptide was added to reaction
    *** reversed by: xPdpk1(R238E) [substitution]
    *** source: 12177059-Fig-5b

===========================================
Copreciptiation requires Ywhaq R56 R60 R127
===========================================

  *** xPdpk1(52-556)[tAb] copptby[WB] xYwhaq[tAb] is detectable
    *** cells: HEK293T<xYwhaq><xPdpk1(52-556)> in BMS
    *** inhibited by: xYwhaq(R56A/R60A) [substitution]
    *** inhibited by: xYwhaq(R127A) [substitution]
    *** source: 12177059-Fig-2a

==============================================
Copreciptiation requires Pdpk1 S241(phos) R238E 
==============================================

  *** xYwhaq[tAb] copptby[WB] xPdpk1(52-556)[tAb] is detectable
    *** cells: HEK293T<xYwhaq><xPdpk1(52-556)> in BMS
    *** inhibited by: xPdpk1(52-556/S241A) [substitution]
    *** unaffected by: xPdpk1(52-556/S410A) [substitution]
    *** unaffected by: xPdpk1(52-556/S549A) [substitution]
    *** source: 12177059-Fig-3b
  *** xYwhaq[tAb] copptby[WB] xPdpk1[tAb] is detectable
    *** cells: HEK293T<xYwhaq><xPdpk1> in BMS
    *** inhibited by: xPdpk1(S241A) [substitution]
    *** partially inhibited by: xPdpk1(R238E) [substitution]
    *** unaffected by: xPdpk1(A239E) [substitution]
    *** unaffected by: xPdpk1(A239Q) [substitution]
    *** enhanced by: xPdpk1(V243P) [substitution]
    *** source: 12177059-Fig-3c
  *** xYwhaq[tAb] copptby[WB] xPdpk1[tAb] is detectable
    *** cells: HEK293T<xYwhaq><xPdpk1> in BMS
    *** inhibited by: xPdpk1(S241A/V243P) [substitution]
    *** enhanced by: xPdpk1(V243P) [substitution]
    *** source: 12177059-Fig-3d

  *** xPdpk1[Ab] copptby[WB] xYwhaq[GST] is detectable
    *** cells: HEK293T<xYwhaq><xPdpk1> in BMS
    *** source: 12177059-Fig-1f
  *** Pdpk1[Ab] copptby[WB] Ywhaq[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 12177059-Fig-1e
  *** Pdpk1[Ab] copptby[WB] Ywhaq[Ab] is detectable
    *** cells: NIH3T3 in BMS
    *** source: 12177059-Fig-1e
  *** xYwhaq[tAb] copptby[WB] xPdpk1[tAb] is detectable
    *** cells: HEK293T<xYwhaq><xPdpk1> in BMS
    *** source: 12177059-Fig-1b

  *** Ywhaq[Ab] copptby[WB] Pdpk1[Ab] is detectable
    *** cells: HEK293T in BMS
    *** repressed by: Smad2 [RNAi]
    *** repressed by: Smad3 [RNAi]
    *** repressed by: Smad4 [RNAi]
    *** repressed by: Smad7 [RNAi]
    *** source: 17327236-Fig-5a
