=================
Kinase Activation
=================

Mkk4-- and/or Mkk7--

  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (times)
    *** cells: mEFs in BMS
    *** times: 0 15++ 30++ 60++ 120+ 240+ min
    *** partially reqs: Mkk4 [KO]
    *** partially reqs: Mkk7 [KO]
    *** reqs: Mkk4 and/or Mkk7 [DKO]
    *** source: 11390361-Fig-5a

  *** xJnk1[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (15 min)
    *** cells: KB in BMS
    *** unaffected by: xMkk7(K165L)"mouseKD" [addition]
    *** source: 9384583-Fig-6b

not Mekk3--

  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (tnr)
    *** cells: mEFs in BMS
    *** does not req: Mekk3 [KO]
    *** source: 14661019(D)

not Tpl2--

  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (tnr)
    *** cells: mEFs in BMS
    *** does not req: Tpl2 [KO]
    *** source: 15833743-Fig-1

not Traf6--

  *** Jnks[Ab]IP IVKA(snr)[32P-ATP] is increased irt Anisomycin (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 30+ 90 min
    *** does not req: Traf6 [KO]
    *** source: 10215628-Fig-5d

Shoc2--  ???

  *** xJnk?[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (tnr)
    *** cells: HEK293 in BMLS
    *** unaffected by: xShoc2 [addition]
    *** source: 10783161-Fig-2d

not Mek5--

  *** xJnk2[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (60 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xMek5(S311A/T315A)"DN" [addition]
    *** source: 10473620-Fig-4e,5a

not Map4k5--

  *** xJnk3[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (15 min)
    *** cells: HEK293T in BMS
    *** does not req: Map4k5 [AntiSense]
    *** source: 9405407-Fig-4b

chemicals

  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (times)
    *** cells: CHO<xInsR> in BMS
    *** times: 0 5 30+ min
    *** unaffected by: PD98059 [addition]
    *** source: 8626428-Fig-5b

no extras

  *** Jnk1[Ab]IP IVKA(Stat3)[32P-ATP] is increased irt Anisomycin (30 min)
    *** cells: COS1 in BMS
    *** source: 10521505-Fig-2a
  *** xJnk1[tAb]IP IVKA(Atf2)[32P-ATP] is increased irt Anisomycin (15 min)
    *** cells: COS7 in BMS
    *** source: 8910292(D)
  *** xJnk1[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (tnr)
    *** cells: COS1 in BMS
    *** source: 8940179-Fig-4
  *** xJnk3[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (15 min)
    *** cells: COS in BMS
    *** source: 8702571-Fig-1b
  *** xJnk3[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (15 min)
    *** cells: HEK293T in BMS
    *** source: 9405407-Fig-2a
  *** xJnk3[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (30 min)
    *** cells: NIH3T3 in BMLS
    *** source: 9006902-Fig-2a
  *** xJnk3[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (tnr)
    *** cells: COS1 in BMS
    *** source: 9003778-Fig-1a
  *** xJnk3[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (tnr)
    *** cells: COS7 in BMS
    *** source: 8999998-Fig-2c
  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (times)
    *** cells: NIH3T3L in BMS "differentiated into adipocytes"
    *** times: 0 5 30+ min
    *** source: 8626428-Fig-3b
  *** Jnks[Ab]IP IVKA(Atf2)[32P-ATP] is increased irt Anisomycin (10 min)
    *** cells: KIT225 in BMS
    *** source: 9169478-Fig-1a
  *** Jnks[Ab]IP IVKA(Elk1)[32P-ATP] is increased irt Anisomycin (30 min)
    *** cells: NIH3T3 in BMLS
    *** source: 8548291-Fig-1c
  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (60 min)
    *** cells: HEK293 in BMS
    *** source: 12228228-Fig-4b
  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Anisomycin (times)
    *** cells: CHO<xInsR> in BMS
    *** times: 0 5 30+ min
    *** source: 8626428-Fig-3b

===========================
Phosphorylation on TPY site
===========================

  *** Jnk1[phosAb] phos(TPY)[phosAb] is increased irt Anisomycin (30 min)
    *** cells: mEFs in BMS
    *** does not req: Jnk2 [KO]
    *** source: 17074809-Fig-1c
  *** Jnk2[phosAb] phos(TPY)[phosAb] is increased irt Anisomycin (30 min)
    *** cells: mEFs in BMS
    *** does not req: Jnk1 [KO]
    *** source: 17074809-Fig-1c
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Anisomycin (20 min)
    *** cells: HEPG2 in BMLS
    *** source: 12458207-Fig-1a
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Anisomycin (30 min)
    *** cells: mEFs in BMS
    *** unaffected by: SB203580 [addition]
    *** source: 15304344-Fig-4a
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Anisomycin (times)
    *** cells: HEK293<xPias2a> in BMS
    *** times: 0 5 15 30+ 60+ 120+ min
    *** source: 16713578-Fig-3a
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Anisomycin (times)
    *** cells: RAW2647 in BMS
    *** times: 0 2 5+ 15+ 30+ 45+ 60+ 90+ min
    *** source: 12881422-Fig-2d
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Anisomycin (times)
    *** cells: rNCMs in BMS
    *** times: 0 30+ 60+ min
    *** source: 17085580(D)

