==========
Relocation
==========

  *** Akt1[Ab] infraction(membrane)[WB] is increased irt Igf1 (10 min)
    *** cells: HELA in BMLS
    *** source: 12654898-Fig-4a

===============
Kinase Activity
===============

Pdpk1--

  *** Akt1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt Igf1 (15 min)
    *** cells: mESCs in BMLS
    *** reqs: Pdpk1 [KO]
    *** unaffected by: kPdpk1(L155E) [substitution]
    *** source: 12912918-Fig-3
  *** Akt1[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt Igf1 (times)
    *** cells: mESCs in BMLS
    *** times: 0 1+ 2++ 5++ 15++ 45++ min
    *** reqs: Pdpk1 [KO]
    *** source: 10801415-Fig-2a

chemicals

  *** Akt1[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt Igf1 (times)
    *** cells: mESCs in BMLS
    *** times: 0 1+ 2++ 5++ 15++ 45++ min
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 10801415-Fig-2a

no extras

  *** Akt1[Ab]IP IVKA(Gsk3b)[32P-ATP] is increased irt Igf1 (30 min)
    *** cells: MCF7 in BMLS
    *** source: 17693255-Fig-S1a

===============
Phosphorylation
===============

not Freud1--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (10 min)
    *** cells: HEK293T in BMLS
    *** does not req: Freud1 [RNAi]
    *** source: 18662999-Fig-5a,5d
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (10 min)
    *** cells: HT1080 in BMLS
    *** does not req: Freud1 [RNAi]
    *** source: 18662999-Fig-5a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (10 min)
    *** cells: HEK293T in BMLS
    *** does not req: Freud1 [RNAi]
    *** source: 18662999-Fig-5a,5d
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (10 min)
    *** cells: HT1080 in BMLS
    *** does not req: Freud1 [RNAi]
    *** source: 18662999-Fig-5a

not Gab1--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ min
    *** does not req: Gab1 [KO]
    *** source: 19401591-Fig-4a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ min
    *** does not req: Gab1 [KO]
    *** source: 19401591-Fig-4a

not Gnai1-- or Gnai3--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 20+ min
    *** does not req: Gnai1 or Gnai3 [KO]
    *** source: 19401591-Fig-2b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 20+ min
    *** does not req: Gnai1 or Gnai3 [KO]
    *** source: 19401591-Fig-2b


not Irs1--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (10 min)
    *** cells: mEFs in BMLS
    *** does not req: Irs1 [KO]
    *** source: 11113178-Fig-7

Parva--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (10 min)
    *** cells: HELA in BMLS
    *** reqs: Parva [RNAi]
    *** source: 12654898-Fig-3b,3f
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (10 min)
    *** cells: HELA in BMLS
    *** reqs: Parva [RNAi]
    *** source: 12654898-Fig-3b,3f

Pdpk1-- phos(308) not phos(S473)

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (15 min)
    *** cells: mESCs in BMLS
    *** does not req: Pdpk1 [KO]
    *** unaffected by: kPdpk1(L155E) [substitution]
    *** source: 12912918-Fig-3
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (15 min)
    *** cells: mESCs in BMLS
    *** reqs: Pdpk1 [KO]
    *** unaffected by: kPdpk1(L155E) [substitution]
    *** source: 12912918-Fig-3
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (30 min)
    *** cells: mESCs in BMLS
    *** reqs: Pdpk1 [KO]
    *** source: 15024086-Fig-5a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (times)
    *** cells: mESCs in BMLS
    *** times: 0 1+ 2+ 5+ 15+ 45+ min
    *** does not req: Pdpk1 [KO]
    *** source: 10801415-Fig-2b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (times)
    *** cells: mESCs in BMLS
    *** times: 0 1+ 2+ 5+ 15+ 45+ min
    *** reqs: Pdpk1 [KO]
    *** source: 10801415-Fig-2b


Rictor--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (times)
    *** cells: mEFs in BMS
    *** times: 0 5+ 10+ 30+ min
    *** reqs: Rictor [KO]
    *** source: 16962829-Fig-4d

Chemicals

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (times)
    *** cells: SHSY5Y in BMS
    *** times: 0+ 10++ 20++ 60++ min
    *** unaffected by: Aicar [pretreatment]
    *** source: 16620785-Fig-6
  *** Akt1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt Igf1 (15 min)
    *** cells: mESCs in BMLS
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** source: 12912918-Fig-3
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (15 min)
    *** cells: mESCs in BMLS
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** source: 12912918-Fig-3
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (15 min)
    *** cells: mESCs in BMLS
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** source: 12912918-Fig-3
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (4 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: LY294002 [addition]
    *** source: 10576742-Fig-2
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (10 min)
    *** cells: HELA in BMLS
    *** inhibited by: LY294002 [addition]
    *** source: 12853467(D)
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (times)
    *** cells: MCF7 in BMLS
    *** times: 0 2+ 20+ min
    *** inhibited by: LY294002 [addition]
    *** source: 10576742-Fig-6a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (10 min)
    *** cells: HELA in BMLS
    *** unaffected by: Rapamycin [addition]
    *** source: 12853467(D)
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (10 min)
    *** cells: HELA in BMLS
    *** unaffected by: U0126 [addition]
    *** source: 12853467(D)
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (30 min)
    *** cells: HEPG2 in BMLS "plated on collagen"
    *** unaffected by: Gefitinib [cotreatment]
    *** enhanced by: BMS345541 [cotreatment]
    *** unaffected by: PD325901 [cotreatment]
    *** unaffected by: PHA818637 [cotreatment]
    *** enhanced by: ZSTK474 [cotreatment]
    *** source: 19953085-Fig-S1
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (30 min)
    *** cells: mESCs in BMLS
    *** unaffected by: Rapamycin [pretreatment]
    *** source: 15024086-Fig-5a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (times)
    *** cells: mESCs in BMLS
    *** times: 0 1+ 2+ 5+ 15+ 45+ min
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 10801415-Fig-2b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (times)
    *** cells: mESCs in BMLS
    *** times: 0 1+ 2+ 5+ 15+ 45+ min
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 10801415-Fig-2b


No Extras

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (20 min)
    *** cells: HEK293 in BMLS
    *** source: 17617058-Fig-4a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (20 min)
    *** cells: HEK293 in BMLS
    *** source: 17617058-Fig-6
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Igf1 (times)
    *** cells: SHSY5Y in BMS
    *** times: 0 10+ 20+ 60+ min
    *** source: 16620785(D)
  *** Akt1[phosAb] phos(T308)[phosAb] is unchanged irt Igf1 (times)
    *** cells: mEFs in BMS
    *** times: 0+ 5+ 10+ 30+ min
    *** source: 16962829-Fig-4d
  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Igf1 (30 min)
    *** cells: MCF7 in BMLS
    *** inhibited by: LY294002 [pretreatment]
    *** source: 11504915-Fig-2c
  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Igf1 (times)
    *** cells: MCF7 in BMLS
    *** times: 0 5 15+ 30+ 45+ 60+ min
    *** source: 11504915-Fig-2b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (30 min)
    *** cells: MCF7 in BMLS
    *** source: 17693255-Fig-S1a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (30 min)
    *** cells: MCF7 in BMLS
    *** source: 17693255-Fig-S1b

longer times

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Igf1 (2 hr)
    *** cells: PANC1 in BMS
    *** inhibited by: LY294002 [addition]
    *** unaffected by: Genistein [addition]
    *** unaffected by: PD98059 [addition]
    *** source: 15485651(D)
