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Phosphorylation on SMANS site
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Nfkb1-- required for stabilization of Tpl2 only

  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 15+ 30 min
    *** reqs: Nfkb1 [KO]
    *** source: 17709378-Fig-1b
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (15 min)
    *** cells: mBMMs<Nfkb1~null><xNfkb> in BMS
    *** reqs: xNfkb1 [omission]
    *** inhibited by: xNfkb1(S927A/S932A) [substitution]
    *** source: 15485931-Fig-4e
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: RAW2647<xNfkb1> in BMS
    *** times: 0 7+ 15++ 30+ min
    *** partially inhibited by: xNfkb1(S927A/S932A) [substitution]
    *** source: 15485931-Fig-4a
  *** Mek1[phosAb] phos(sitenr)[phosAb] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 15+ 30+ min
    *** reqs: Nfkb1 [KO]
    *** source: 12667451-Fig-2a

not Nfkb2--

  *** Mek1[phosAb] phos(sitenr)[phosAb] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 15+ 30+ min
    *** does not req: Nfkb2 [KO]
    *** source: 12667451-Fig-2f

Tpl2--

  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (30 min)
    *** cells: mPECs in BMLS
    *** reqs: Tpl2 [KO]
    *** source: 11163183-Fig-5a

  Tpl2 phos(S400) not phos(S413) kinase activity

  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: mBMMs<Tpl2~null><xTpl2> in BMS
    *** times: 0 15++ 30+ min
    *** reqs: xTpl2 [omission]
    *** inhibited by: xTpl2(S400A) [substitution]
    *** unaffected by: xTpl2(S413A) [substitution]
    *** inhibited by: xTpl2(D270A)"KD" [substitution]
    *** source: 17709378-Fig-4a
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (15 min)
    *** cells: RAW2647<xTpl2> in BMS
    *** unaffected by: xTpl2(S400A) [substitution]
    *** source: 17709378-Fig-6d

  Tpl2 kinase activity

  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: mBMMs<Nfkb1~null><xTpl2> in BMS
    *** times: 0 15++ 30+ min
    *** reqs: xTpl2 [omission]
    *** inhibited by: xTpl2(D207A) [substitution]
    *** source: 17709378-Fig-1b
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: RAW2647<xTpl2> in BMS
    *** times: 0 7+ 15++ 30+ min
    *** partially inhibited by: xTpl2(mnr)"KD" [substitution]
    *** source: 15485931-Fig-3a

chemicals

  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (7 min)
    *** cells: RAW2647<xTpl2> in BMS
    *** inhibited by: BMS345541 [pretreatment]
    *** inhibited by: IKK2InhibitorIV [pretreatment]
    *** source: 17709378-Fig-5c
  *** Mek1[phosAb] phos(sitenr)[phosAb] is increased irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** inhibited by: PS1145 [pretreatment]
    *** source: 15199157-Fig-3a
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** inhibited by: Bay117082 [pretreatment]
    *** source: 15485931-Fig-5b
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 7++ 15++ 30+ min
    *** inhibited by: MG132 [pretreatment]
    *** source: 15485931-Fig-2c
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 7++ 15++ 30+ min
    *** partially inhibited by: Bay117082 [pretreatment]
    *** source: 15485931-Fig-5a
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: RAW2647 in BMS
    *** times: 0 7+ 15++ 30+ min
    *** partially inhibited by: MG132 [pretreatment]
    *** source: 15485931-Fig-3b

no extras

  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 10+ 30+ 60+ min
    *** source: 15169888-Fig-9a
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 7++ 15++ 30+ min
    *** source: 15485931-Fig-1a
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: RAW2647 in BMS
    *** times: 0 7+ 15++ 30+ min
    *** source: 15485931-Fig-3a
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Lps (times)
    *** cells: RAW2647 in BMS
    *** times: 0 7+ 15++ 30+ min
    *** source: 15485931-Fig-4a
