=========================
Sos1{Yphos} is a Rac1Gef
=========================

  *** rRac1 GDP-dissociation[3H-GDP] is increased by xSos1{Yphos}[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAbl1(del(SH3))"CA"> in BMS
    *** inhibited by: xSos1[tAb]IP [substitution]
    *** source: 15039778-Fig-2d
  *** rRac1 GDP-dissociation[3H-GDP] is increased by xSos1{Yphos}[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAbl1(del(SH3))"CA"> in BMS
    *** inhibited by: xSos1[tAb]IP [substitution]
    *** comment: inhibited by rSos1{Yphos} PTPase-treatment
    *** source: 15039778-Fig-3a

===================================================================
Sos1 Rac1Gef activity is increased by Abi1 and Eps8
===================================================================

  *** Sos1[Ab] snaggedby[WB] rRac1{NucFree}[GST] is detectable
    *** cells: mEFs<Eps8~null><xEps8> in BMS
    *** inhibited by: rAbi1(414-425)"Eps8-binding-site" [addition]
    *** inhibited by: rSos1(1147-1158)"Eps8-binding-site" [addition]
    *** source: 11777939-Fig-7c
  *** Sos1[Ab] snaggedby[WB] rRac1{NucFree}[GST] is detectable
    *** cells: mEFs<Eps8~null><xEps8> in BMS
    *** reqs: xEps8 [omission]
    *** source: 11777939-Fig-7c

  *** xSos1[Ab]IP IVGefA(Rac1)[3H-GDP] is increased itpo (xAbi1 + xEps8)
    *** cells: COS7 in BMS
    *** source: 11777939-Fig-7b

  *** xEps8[Ab]IP IVGefA(Rac1)[3H-GDP] is detectable
    *** cells: COS7<xAbi1><xSos1> in BMS
    *** source: 11777939-Fig-7b

======================================================
Rac1{GTP} is increased in the presence of Abi1 and Eps8
======================================================

  *** Rac1[Ab] GTP-association[BDPD] is increased itpo xAbi1
    *** cells: mEFs in BMS
    *** reqs: Eps8 [KO]
    *** inhibited by: xAbi1(D420A/Y421A)"Eps8-binding-site-mutant" [substitution]
    *** source: 11777939-Fig-5

  *** xRac1[tAb] GTP-association[BDPD] is increased itpo xAbi1
    *** cells: COS7 in BMS
    *** inhibited by: xAbi1(Y407F) [substitution]
    *** inhibited by: xAbi1(mnr)"Eps8-binding-mut" [substitution]
    *** source: 12515821-Fig-4a
  *** xRac1[tAb] GTP-association[BDPD] is increased itpo xAbi1
    *** cells: mEFs<Pik3r1~null><Pik3r2~null><xPik3r1> in BMLS
    *** reqs: xPik3r1 [omission]
    *** source: 12515821-Fig-4b

