======================
Direct Phosphorylation
======================

  *** rAkt1[pYAb] Yphos[pYAb] is increased by rPtk6
    *** cells: none
    *** comment: sites were shown to be Y215 and Y326 by MS
    *** source: 20606012-Fig-1c
  *** rAkt1[pYAb] Yphos[pYAb] is increased by rPtk6
    *** cells: none
    *** partially inhibited by: rAkt1(Y315F/T326F) [substitution]
    *** source: 20606012-Fig-3d

==================================================
Phosphorylation in response to Ptk6 overexpression
==================================================

  *** xAkt1[Ab]IP Yphos[pYAb] is increased itpo xPtk6(Y447F)
    *** cells: SYF<Src~null><Yes1~null><Fyn~null><xAkt1> in BMS
    *** source: 20606012-Fig-5c
  *** xAkt1[tAb] Yphos[pYAb-ppt] is increased itpo xPtk6(Y447F)
    *** cells: HEK293<xAkt1> in BMS
    *** source: 20606012-Fig-1c
  *** xAkt1[tAb] Yphos[pYAb-ppt] is increased itpo xPtk6(Y447F)
    *** cells: COS1<xAkt1><xPtk6(Y447F)> in BMLS
    *** inhibited by: xPtk6(K219M)"KD" [substitution]
    *** source: 15539407-Fig-3c
  *** xAkt1[tAb]IP Yphos[pYAb] is increased itpo xPtk6(Y447F)
    *** cells: COS1<xAkt1><xPtk6(Y447F)> in BMLS
    *** inhibited by: xPtk6 [substitution]
    *** inhibited by: xPtk6(K219M)"KD" [substitution]
    *** source: 15539407-Fig-3b

===================
Protein Association
===================

  *** xAkt1[tAb] copptby[WB] xPtk6[tAb] is detectable
    *** cells: HEK293<xAkt1><xPtk6> in BMS
    *** partially inhibited by: xAkt1(Y315F) [substitution]
    *** partially inhibited by: xAkt1(T326F) [substitution]
    *** inhibited by: xAkt1(Y315F/T326F) [substitution]
    *** partially inhibited by: xAkt1(Y315E/T326E) [substitution]
    *** source: 20606012-Fig-4a
  *** xAkt1[tAb] snaggedby[WB] rPtk6[GST] is detectable
    *** cells: HEK293<xAkt1> in BMS
    *** unaffected by: rPtk6(mnr)"SH3" [substitution]
    *** partially inhibited by: rPtk6(mnr)"SH2" [substitution]
    *** unaffected by: rPtk6(mnr)"SH3/SH2" [substitution]
    *** source: 20606012-Fig-4d
  *** xAkt1[tAb]IP Yphos[pYAb] is increased itpo xPtk6(Y447F)
    *** cells: HEK293<xAkt1> in BMS
    *** source: 20606012-Fig-1b
  *** xAkt1[tAb]IP Yphos[pYAb] is increased itpo xPtk6(Y447F)
    *** cells: HEK293<xAkt1> in BMS
    *** inhibited by: xAkt1(Y315F/T326F) [substitution]
    *** unaffected by: xAkt1(Y215F) [substitution]
    *** source: 20606012-Fig-3c
  *** xAkt1[tAb]IP Yphos[pYAb] is increased itpo xPtk6(Y447F)
    *** cells: HEK293<xAkt1> in BMS
    *** partially inhibited by: xAkt1(Y315F) [substitution]
    *** partially inhibited by: xAkt1(T326F) [substitution]
    *** inhibited by: xAkt1(Y315F/T326F) [substitution]
    *** partially inhibited by: xAkt1(Y315E/T326E) [substitution]
    *** source: 20606012-Fig-4c
  *** Akt1[Ab] copptby[WB] xPtk6[Ab] is detectable
    *** cells: COS1<xPtk6> in BMS
    *** source: 15539407-Fig-1a
  *** xAkt1[tAb] copptby[WB] xPtk6[Ab] is detectable
    *** cells: COS1<xPtk6><xAkt1> in BMS
    *** unaffected by: xAkt1(mnr)"KD" [substitution]
    *** unaffected by: xAkt1(P424A/P427A)"PxxP" [substitution]
    *** inhibited by: xAkt1(del(4-129))"delN" [substitution]
    *** source: 15539407-Fig-1d
  *** xPtk6[tAb] copptby[WB] xAkt1[tAb] is detectable
    *** cells: HEK293<xAkt1><xPtk6> in BMS
    *** inhibited by: xAkt1(P424A/P427A) [substitution]
    *** unaffected by: xAkt1(P467A/P470A) [substitution]
    *** source: 20606012-Fig-4e
  *** xPtk6[Ab] copptby[WB] Akt1[Ab] is detectable
    *** cells: COS1<xPtk6> in BMS
    *** source: 15539407-Fig-2b
  *** Ptk6[Ab] copptby[WB] Akt1[tAb] is detectable
    *** cells: HACAT in BMLS
    *** source: 15539407-Fig-2a
  *** Ptk6[Ab] copptby[WB] Akt1[tAb] is detectable
    *** cells: T47D in BMS
    *** source: 15539407-Fig-1c
  *** xPtk6[Ab] copptby[WB] xAkt1(mnr)"KD"[tAb] is detectable
    *** cells: COS1<xPtk6><xAkt1> in BMLS
    *** unaffected by: xPtk6(Y447F) [substitution]
    *** enhanced by: xPtk6(K219M)"KD" [substitution]
    *** source: 15539407-Fig-2c
  *** xPtk6[Ab] copptby[WB] xAkt1[tAb] is detectable
    *** cells: COS1<xPtk6><xAkt1> in BMS
    *** source: 15539407-Fig-1b

