===============
Kinase Activity
===============

Mek1--

  *** xRsk3[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk3> in BMLS
    *** inhibited by: xMek1(S218A) [substitution]
    *** unaffected by: xMek1(S218E/S222E) [substitution]
    *** source: 8939914-Fig-2

not Msk1--

  *** Rsk3[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt Egf (times)
    *** cells: mESCs in BMLS
    *** times: 0 10+ 20 30 min
    *** does not req: Msk1 [KO]
    *** source: 11018520-Fig-4b
  *** Rsk3[Ab]IP IVKA(peptide)[32P-ATP] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** does not req: Msk2 [KO]
    *** does not req: Msk1 [KO]
    *** does not req: Msk1 or Msk2 [DKO]
    *** source: 11909979-Fig-4b

Rsk3 features

  *** xRsk3[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** bkg inhibited by: xRsk3(S386K) [substitution]
    *** source: 10856237-Fig-4a
  *** xRsk3[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk3> in BMLS
    *** inhibited by: xRsk3(S386A) [substitution]
    *** source: 10480933-Fig-8a
  *** xRsk3[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk3> in BMLS
    *** partially inhibited by: xRsk3(S227E) [substitution]
    *** source: 10480933-Fig-8a
  *** xRsk3[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk3> in BMLS
    *** partially inhibited by: xRsk3(S369A) [substitution]
    *** inhibited by: xRsk3(S369A/T577A) [substitution]
    *** source: 10480933-Fig-9a
  *** xRsk3[tAb]IP IVKA(S6peptide)[32P-ATP] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** inhibited by: xRsk3(R119A) [substitution]
    *** source: 12374740-Fig-8a

Chemicals

  *** xRsk3[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk3> in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** source: 8939914-Fig-3b
  *** Rsk2[Ab]IP IVKA(peptide)[32P-ATP] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** unaffected by: SB203580 [addition] no-bkg-control
    *** source: 11909979-Fig-3b
  *** Rsks[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** unaffected by: SB203580 [pretreatment]
    *** source: 9687510-Fig-5b

No extras

  *** xRsk3[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk3> in BMLS
    *** source: 8939914-Fig-1b
  *** xRsk3[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** source: 10856237-Fig-3a
  *** xRsk3[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** source: 10856237-Fig-11a
  *** xRsk3[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk3> in BMLS
    *** source: 10480933-Fig-5
  *** xRsk3[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk3> in BMLS
    *** source: 10480933-Fig-5
  *** xRsk2[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293<xRsk2> in BMLS
    *** source: 12832467-Fig-6c
  *** xRsk2[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (times)
    *** cells: HEK293<xRsk2> in BMLS
    *** times: 0 10+++ 30++ 60+ 90+ 120+ min
    *** source: 12832467-Fig-7a

=======================
Phosphorylation on S227
=======================

requires phos(S386)

  *** xRsk3[tAb]IP phos(S227)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** inhibited by: xRsk3(S386K) [substitution]
    *** source: 10856237-Fig-4b

no extras/other

  *** xRsk3[tAb]IP phos(S227)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** source: 10856237-Fig-3c
  *** xRsk3[tAb]IP phos(S227)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** unaffected by: xRsk3(R119A) [substitution]
    *** source: 12374740-Fig-8b

=======================
Phosphorylation on S369
=======================

does not req phos on S227 or S386

  *** xRsk3[tAb]IP phos(S369)[phosAb] is increased irt Egf (15 min)
    *** cells: COS7<xRsk2> in BMLS
    *** unaffected by: xRsk3(S227E) [substitution]
    *** unaffected by: xRsk3(S386A) [substitution]
    *** source: 10480933-Fig-8b
  *** xRsk3[tAb]IP phos(S369)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** unaffected by: xRsk3(S386K) [substitution]
    *** source: 10856237-Fig-4d

requires something in 390-740  Cterm kinase??

  *** xRsk3[tAb]IP phos(S369)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** inhibited by: xRsk3(1-380) [substitution]
    *** inhibited by: xRsk3(1-389) [substitution]
    *** inhibited by: xRsk3(1-389/S386A) [substitution]
    *** source: 10856237-Fig-3e

=======================
Phosphorylation on S386
=======================

  *** xRsk3[tAb]IP phos(S386)[phosAb] is increased irt Egf (15 min)
    *** cells: COS7<xRsk3> in BMLS
    *** unaffected by: xRsk3(S369A) [substitution]
    *** inhibited by: xRsk3(S369A/T577A) [substitution]
    *** source: 10480933-Fig-9b

no extras/other

  *** xRsk3[tAb]IP phos(S386)[phosAb] is increased irt Egf (15 min)
    *** cells: COS7<xRsk3> in BMLS
    *** source: 10480933-Fig-8b
  *** xRsk3[tAb]IP phos(S386)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** source: 10856237-Fig-3b
  *** xRsk3[tAb]IP phos(S386)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** unaffected by: xRsk3(R119A) [substitution]
    *** source: 12374740-Fig-8b

=======================
Phosphorylation on T365
=======================

requires something in 390-740  Cterm kinase??

  *** xRsk3[tAb]IP phos(T365)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** inhibited by: xRsk3(1-380) [substitution]
    *** inhibited by: xRsk3(1-389) [substitution]
    *** inhibited by: xRsk3(1-389/S386A) [substitution]
    *** source: 10856237-Fig-3e

does not require phos(S386)

  *** xRsk3[tAb]IP phos(T365)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** unaffected by: xRsk3(S386K) [substitution]
    *** source: 10856237-Fig-4c

no extras

  *** xRsk3[tAb]IP phos(T365)[phosAb] is increased irt Egf (20 min)
    *** cells: COS7<xRsk3> in BMLS
    *** source: 10856237-Fig-3d

=====================
Total Phosphorylation
=====================

  *** xRsk3(1-360)[tAb]IP phos[32Pi] is unchanged irt Egf (15 min)
    *** cells: COS7<xRsk3(1-360)> in BMLS
    *** source: 10480933-Fig-4


