================================================================
Smad2 return to cytoplasm does not require Crm1 (chemicals only)
================================================================

  *** Smad23s[Ab] locatedin(cytoplasm)[IHC] is increased irt SB431542 (120 min)
    *** cells: HACAT in BMS "treated with CHX" "treated with Tgfb1 for 60 min"
    *** unaffected by: LMB [addition]
    *** source: 12191474-Fig-5c
  *** xSmad2[MS2] nuc-export[NEreporter] is unchanged irt LMB (4 hr)
    *** cells: HEK293T in BMS
    *** source: 12191473-Fig-6e
  *** xSmad2[tAb] locatedin(nucleus)[IHC] is unchanged irt LMB (4 hr)
    *** cells: HELA in BMS
    *** source: 12191473-Fig-3c

============================================================
Smad2 return to cytoplasm does not require protein synthesis
============================================================

  *** Smad23s[Ab] locatedin(cytoplasm)[IHC] is increased irt SB431542 (120 min)
    *** cells: HACAT in BMS "treated with CHX" "treated with Tgfb1 for 60 min"
    *** inhibited by: NaN3 + 2DOG [addition]
    *** source: 12191474-Fig-6
  *** Smad23s[Ab] locatedin(cytoplasm)[IHC] is increased irt SB431542 (times)
    *** cells: BXPC3<Smad4~null> in BMS "treated with CHX" "treated with Tgfb1 for 60 min"
    *** times: 0 30+ 60++ 120+++ 180+++ min
    *** source: 12191474-Fig-5a
  *** Smad23s[Ab] locatedin(cytoplasm)[IHC] is increased irt SB431542 (times)
    *** cells: HACAT in BMS "treated with CHX" "treated with Tgfb1 for 60 min"
    *** times: 0 30+ 60++ 120+++ 180+++ min
    *** source: 12191474-Fig-3b

===============================================
Smad2 regions required for nuclear export
are located in the Nup153 binding region
===============================================

  *** xSmad2(mnr)"NLS-fused"[tAb] nuc-export[HeterokaryonAssay] is detectable
    *** cells: GSN2 in BMS
    *** inhibited by: xSmad2(V398R) [substitution]
    *** unaffected by: xSmad2(P445H) [substitution]
    *** inhibited by: xSmad2(W448A) [substitution]
    *** inhibited by: xSmad2(C380R/L382R) [substitution]
    *** inhibited by: xSmad2(L382R/K383A) [substitution]
    *** source: 12191473-Fig-4a
  *** xSmad2[MS2] nuc-export[NEreporter] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xSmad2(V398R) [substitution]
    *** unaffected by: xSmad2(P445H) [substitution]
    *** inhibited by: xSmad2(W448A) [substitution]
    *** unaffected by: xSmad2(C380R/L382R) [substitution]
    *** unaffected by: xSmad2(L382R/K383A) [substitution]
    *** source: 12191473-Fig-6c
 ----------------------------------------------------------------
  *** xNup153(1156-1476)[tAb] snaggedby[WB] rSmad2(185-467)"LMH2"[GST] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: rSmad2((185-467)/W448A) [substitution]
    *** unaffected by: rSmad2((185-467)/P445H) [substitution]
    *** inhibited by: rSmad2((185-467)/V398R) [substitution]
    *** unaffected by: rSmad2((185-467)/C380R/L382R) [substitution]
    *** unaffected by: rSmad2((185-467)/L382R/K383A) [substitution]
    *** source: 12191473-Fig-2e

================================================
Smad2 return to cytoplasm is inhibited by xFoxh1
================================================

  *** xSmad2[MS2] nuc-export[NEreporter] is decreased itpo xFoxh1
    *** cells: HEK293T in BMS
    *** source: 12191473-Fig-6d
  *** xSmad2(mnr)"NLS-fused"[tAb] nuc-export[HeterokaryonAssay] is decreased itpo xFoxh1
    *** cells: GSN2 in BMS
    *** source: 12191473-Fig-5d




  *** xSmad2[MS2] nuc-export[NEreporter] is decreased itpo xNup153(1156-1476)"DN"
    *** cells: HEK293T in BMS
    *** source: 12191473-Fig-6e
  *** xSmad2[MS2] nuc-export[NEreporter] is decreased itpo xNup214(1600-2091)"DN"
    *** cells: HEK293T in BMS
    *** source: 12191473-Fig-6d
  *** xSmad2[MS2] nuc-export[NEreporter] is decreased itpo xNup214(1864-2091)"DN"
    *** cells: HEK293T in BMS
    *** source: 12191473-Fig-6d
