=======================
Phosphorylation on S317
=======================

Atr--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (2 hr)
    *** cells: HELA in BMS
    *** reqs: Atr [RNAi]
    *** source: 16431910-Fig-1a
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Atr [RNAi]
    *** source: 16327781-Fig-1b
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Atr [RNAi]
    *** source: 16327781-Fig-S1a
  *** Chek1[phosAb] phos(S317)[phosAb] is unchanged irt UV (1 hr)
    *** cells: hFibros<Atr~fmut> in BMS
    *** source: 16327781-Fig-1a

Atrip--

  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt UV (1 hr)
    *** cells: HEK293T<Atrip~kd><xAtrip> in BMS
    *** reqs: xAtrip [omission]
    *** inhibited by: xAtrip(1-768)"delC" [substitution]
    *** source: 15758953-Fig-5f

not Atm--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (2 hr)
    *** cells: HELA in BMS
    *** does not req: Atm [RNAi]
    *** source: 16431910-Fig-1a
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (1 hr)
    *** cells: hFibros<Atm~fmut> in BMS
    *** source: 16327781-Fig-1a

not Claspin--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Claspin [RNAi]
    *** source: 16327781-Fig-S1b

not Mre11--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (2 hr)
    *** cells: HELA in BMS
    *** does not req: Mre11 [RNAi]
    *** source: 16431910-Fig-1a
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (2 hr)
    *** cells: U2OS in BMS
    *** does not req: Mre11 [RNAi]
    *** source: 16431910-Fig-S3b
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (1 hr)
    *** cells: hFibros<Mre11~fmut><xMre11> in BMS
    *** does not req: xMre11 [omission]
    *** unaffected by: xMre11(mnr)"CIA" [substitution]
    *** source: 16327781-Fig-4a

not Nbs1--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (1 hr)
    *** cells: hFibros<Nbs1~fmut><xNbs1> in BMS
    *** does not req: xNbs1 [omission]
    *** unaffected by: xNbs1(S278A/S343A/S397A) [substitution]
    *** source: 16327781-Fig-2c

not Rad50--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (2 hr)
    *** cells: U2OS in BMS
    *** does not req: Rad50 [RNAi]
    *** source: 16431910-Fig-S3b

not Topbp1--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Topbp1 [RNAi]
    *** source: 16327781-Fig-S1b

chemicals

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (1 hr)
    *** cells: U2OS in BMS
    *** unaffected by: KU55933 [pretreatment]
    *** source: 16327781-Fig-1b

no extras

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt UV (1 hr)
    *** cells: HELA in BMS
    *** source: 16327781-Fig-1a


=======================
Phosphorylation on S345
=======================

not Atm--

  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt UV (90 min)
    *** cells: GM1588<Atm~fmut> in BMS
    *** comment: signal is similar to that in cells from normal fibros
    *** source: 16087684-Fig-2b

Atr--

  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt UV (times)
    *** cells: DK0061<Atr~fmut> in BMS
    *** times: 0 30+ 90+ min
    *** comment: signal is significantly lower than that in cells from normal lymphoblasts
    *** source: 16087684-Fig-2b

Mdc1--  ???

  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt UV (3 hr)
    *** cells: NotReported in BMS
    *** partially reqs: Mdc1 [RNAi]
    *** source: 12607005-Fig-5e

Rad50--

  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt UV (times)
    *** cells: HCT116 in BMS
    *** times: 0 30+ 90+ min
    *** partially reqs: Rad50 [RNAi]
    *** comment: Rad50 KD also causes loss of Nbs1 or Mre11 prot-exp
    *** source: 16087684-Fig-2a
  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt UV (times)
    *** cells: HEK293T in BMS
    *** times: 0 30+ 90+ min
    *** reqs: Rad50 [RNAi]
    *** comment: Rad50 KD also causes partial loss of Nbs1 and total loss Mre11 prot-exp
    *** source: 16087684-Fig-2a


no extras

  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt UV (90 min)
    *** cells: hFibros in BMS
    *** source: 16087684-Fig-2b
  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt UV (times)
    *** cells: hFibros in BMS
    *** times: 0 30+ 90+ min
    *** source: 16087684-Fig-2b

