==================
Direct Sumoylation
==================

  *** rElk1 upshift[WBMS] is increased by (rSumo1 + rSae1 + rUba2 + rUbe2i)
    *** cells: none
    *** reqs: rSae1 and/or rUba2 [omission]
    *** reqs: rSumo1 [omission]
    *** reqs: rUbe2i [omission]
    *** source: 12887893-Fig-4b
  *** rElk1 upshift[WBMS] is increased by (rSumo1 + rSae1 + rUba2 + rUbe2i)
    *** cells: none
    *** reqs: rSumo1 [omission]
    *** inhibited by: rElk1(del(229-251)) [substitution]
    *** partially inhibited by: rElk1(K249R) [substitution]
    *** inhibited by: rElk1(K230R/K249R) [substitution]
    *** source: 12887893-Fig-4c
  *** rElk1[35S] upshift[WBMS] is increased by (rUbe2i + rSumo1)
    *** cells: none
    *** comment: reaction also contains HELA cell fractions containing Sumo E1 activity
    *** inhibited by: rElk1(K230R/K249R/K254R) [substitution]
    *** source: 15210726-Fig-1b
  *** rElk1[35S] upshift[WBMS] is increased by (rUbe2i + rSumo1)
    *** cells: none
    *** comment: reaction also contains HELA cell fractions containing Sumo E1 activity
    *** unaffected by: rElk1(K230R) [substitution]
    *** unaffected by: rElk1(K249R) [substitution]
    *** unaffected by: rElk1(K254R) [substitution]
    *** source: 15210726-Fig-1c
  *** rElk1[35S] upshift[WBMS] is increased by (rUbe2i + rSumo2)
    *** cells: none
    *** comment: reaction also contains HELA cell fractions containing Sumo E1 activity
    *** inhibited by: rElk1(K230R/K249R/K254R) [substitution]
    *** source: 15210726-Fig-1b

===================================
Requirement for Elk1 K230/K249/K254
===================================

  *** xElk1[Ab] sumo[tSuAb-ppt] is detectable
    *** cells: HELA<xSumo1> in BMS
    *** inhibited by: xElk1(K230R/K249R/K254R) [substitution]
    *** source: 15210726-Fig-2a
  *** xElk1[Ab] sumo[tSuAb-ppt] is detectable
    *** cells: HELA<xSumo2> in BMS
    *** inhibited by: xElk1(K230R/K249R/K254R) [substitution]
    *** source: 15210726-Fig-2a
  *** xElk1[Ab] sumo[tSuAb-ppt] is detectable
    *** cells: HELA<xSumo2> in BMS
    *** partially inhibited by: rElk1(K230R) [substitution]
    *** partially inhibited by: rElk1(K249R) [substitution]
    *** partially inhibited by: rElk1(K254R) [substitution]
    *** inhibited by: xElk1(K230R/K249R/K254R) [substitution]
    *** source: 15210726-Fig-2d
  *** xElk1[Ab] sumo[tSuAb-ppt] is detectable
    *** cells: HELA<xSumo3> in BMS
    *** inhibited by: xElk1(K230R/K249R/K254R) [substitution]
    *** source: 15210726-Fig-2a

  *** xElk1[His]IP sumo[tSuAb] is detectable
    *** cells: COS7<xElk1><xSumo1> in BMS
    *** inhibited by: rElk1(K249R) [substitution]
    *** inhibited by: rElk1(K230R/K249R) [substitution]
    *** source: 12887893-Fig-4d
  *** xElk1[His]IP sumo[tSuAb] is detectable
    *** cells: COS7<xSumo2> in BMS
    *** inhibited by: rElk1(K249R) [substitution]
    *** inhibited by: rElk1(K230R/K249R) [substitution]
    *** source: 12887893-Fig-4d
