==============================
Direct Phosphorylation on S674
==============================

  *** rMlk3(K144A)"KD"[32P] phos[32P-ATP] is increased by rAkt1
    *** cells: none
    *** inhibited by: rMlk3((1-386)/K144A) [substitution]
    *** unaffected by: rMlk3(511-847) [substitution]
    *** inhibited by: rMlk3((511-847)/S674A) [substitution]
    *** source: 12458207-Fig-3b

================================================
Phosphorylation in response to Akt1 coexpression
================================================

  *** xMlk3(K144A)[tAb]IP phos[32Pi] is increased itpo xAkt1(myr)
    *** cells: HEK293<xMlk3(K144A)> in BMLS
    *** inhibited by: rMlk3(K144A/S674A) [substitution]
    *** source: 12458207-Fig-3c

==========================================================
Mlk2 Phosphorylation on S674 decreases its kinase activity
==========================================================

  *** xMlk3[tAb]IP IVKA(Mkk7)[32P-ATP] is decreased itpo xAkt1
    *** cells: HEK293<xMlk3> in BMLS
    *** source: 12458207-Fig-2e
  *** xMlk3(S674A)[tAb]IP IVKA(Mkk7)[32P-ATP] is detectable-but unchanged itpo xAkt1
    *** cells: HEK293<xMlk3(S674A)> in BMLS
    *** unaffected by: Ins (30 min) [stim]
    *** source: 12458207-Fig-3d

=====================
Mlk3-Akt1 interaction
=====================

  *** xAkt1[tAb] copptby[WB] xMlk3[GST] is detectable
    *** cells: HEPG2<xAkt1><xMlk3> in BMLS
    *** inhibited by: xMlk3(1-366) [substitution]
    *** unaffected by: xMlk3(511-847) [substitution]
    *** source: 12458207-Fig-2b
