================
kinase activity
================

  *** Mlk3[Ab]IP IVKA(Mkk7)[32P-ATP] is increased irt Tnf (30 min)
    *** cells: MCF7 in BMS
    *** inhibited by: Geldanamycin [addition]
    *** source: 15001580(D)

===============
phosphorylation
===============

  *** Mlk3[phosAb] phos(T277/S281)[phosAb] is increased irt Tnf (times)
    *** cells: SKOV3 in BMS
    *** times: 0 1+ 3++ 5++ 7++ 9++ 15+ min
    *** source: 19586614-Fig-1a
  *** Mlk3[phosAb] phos(T277/S281)[phosAb] is increased irt Tnf (times)
    *** cells: HT29 in BMS
    *** times: 0 1+ 3+ 5+ 7+ 9 15+ min
    *** source: 19586614-Fig-1b
  *** Mlk3[phosAb] phos(T277/S281)[phosAb] is increased irt Tnf (times)
    *** cells: HEK293 in BMS
    *** times: 0 5+ 10++ 20+ min
    *** reqs: Traf6 [RNAi]
    *** source: 19586614-Fig-4


==============
ubiquitination
==============

  *** Mlk3[Ab]IP ubiq[UbAb] is increased irt Tnf (5 min)
    *** cells: HEK293 in BMS
    *** source: 19586614-Fig-5c
  *** Mlk3[Ab]IP ubiq[UbAb] is increased irt Tnf (times)
    *** cells: HEK293 in BMS
    *** times: 0+ 1+++ 5+ 10++ 20 min
    *** source: 19586614-Fig-5a
  *** Mlk3[Ab] ubiq[UbAb-ppt] is decreased irt Tnf (times)
    *** cells: HEK293 in BMS
    *** times: 0+ 5+ 7 10 min
    *** source: 19586614-Fig-5d
  *** Mlk3[Ab] copptby[WB] Traf6[Ab] is increased irt Tnf (times)
    *** cells: SKOV3 in BMS
    *** times: 0 1+ 3++ 5++ 7++ 9++ 11++ 13++ 15++ min
    *** source: 19586614-Fig-3b
  *** Mlk3[Ab]IP ubiq[UbAb] is unchanged irt Tnf (times)
    *** cells: SKOV3 in BMS
    *** times: 0+ 1+ 5+ 10+ 20+ min
    *** source: 19586614-Fig-5b

======
coppts  evidence for relocation to TnfR1 irt Tnf
======

  *** Mlk3[Ab] copptby[WB] Traf2[Ab] is increased irt Tnf (times)
    *** cells: SKOV3 in BMS
    *** times: 0+ 1++ 3++ 5+ 7 9 11 15+ min
    *** source: 19586614-Fig-3a

