====================================================
Proteins required for IVKA, phos(S473) or phos(T308)
====================================================

not Abi1--

  *** xAkt1[tAb]IP phos(sitenr)[phosAb] is unchanged irt Egf (5 min)
    *** cells: COS in BMLS
    *** unaffected by: xAbi1 [addition]
    *** source: 11003655(D)

not Cdc42--

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (tnr)
    *** cells: COS7 in BMLS
    *** unaffected by: xCdGap [addition]
    *** source: 15034142-Fig-5e

Dok2--  ???

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0 2+ 5+ 10+ min
    *** inhibited by: xDok2 [addition]
    *** source: 15831486-Fig-4a

not Erbb2--

  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMLS
    *** times: 0 2++ 10+ min
    *** does not req: Erbb2 [RNAi]
    *** source: 16041367-Fig-3a

Freud1--  for kinase activity

  *** Akt1[Ab]IP IVKA(Gsk3?)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HT1080 in BMLS
    *** partially reqs: Freud1 [RNAi]
    *** source: 18662999-Fig-3e

  not for phos(S473)

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (10 min)
    *** cells: HEK293T in BMLS
    *** does not req: Freud1 [RNAi]
    *** source: 18662999-Fig-5a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (10 min)
    *** cells: HEK293T in BMS
    *** does not req: Freud1 [RNAi]
    *** source: 18662999-Fig-4a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (10 min)
    *** cells: HT1080 in BMLS
    *** does not req: Freud1 [RNAi]
    *** source: 18662999-Fig-5a

  for phos(T308)

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (10 min)
    *** cells: HEK293T in BMLS
    *** partially reqs: Freud1 [RNAi]
    *** source: 18662999-Fig-5a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (10 min)
    *** cells: HT1080 in BMLS
    *** partially reqs: Freud1 [RNAi]
    *** source: 18662999-Fig-3e
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (10 min)
    *** cells: HT1080 in BMLS
    *** partially reqs: Freud1 [RNAi]
    *** source: 18662999-Fig-4g
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (10 min)
    *** cells: HT1080 in BMLS
    *** partially reqs: Freud1 [RNAi]
    *** source: 18662999-Fig-5a

Gab1--

  *** xAkt1[tAb]IP IVKA(HistH2B)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO<xGab1> in BMLS
    *** unaffected by: xGab1(Y627F) [substitution]
    *** inhibited by: xGab1(Y447F/Y472F/Y589F) [substitution]
    *** source: 11134009-Fig-6d
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ min
    *** reqs: Gab1 [KO]
    *** source: 19401591-Fig-4a


Gab2-- ???

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: rHepatocytes in BMLS
    *** enhanced by: xGab2 [addition]
    *** enhanced by: xGab2(Y603F/Y632F) [addition]
    *** source: 12464621-Fig-8b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: rHepatocytes in BMLS
    *** enhanced by: xGab2 [addition]
    *** unaffected by: xGab2(del(348-355)) [addition]
    *** source: 12464621-Fig-6b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ min
    *** reqs: Gab1 [KO]
    *** source: 19401591-Fig-4a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 10 20+ min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** reqs: Gnai1 and/or Gnai3 [RNAi]
    *** source: 19401591-Fig-3a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gnai1~null><Gnai3~null><xGnai1> in BMLS
    *** times: 0 10+ 20+ min
    *** reqs: xGnai1 [omission]
    *** source: 19401591-Fig-3b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gnai1~null><Gnai3~null><xGnai3> in BMLS
    *** times: 0 10+ 20+ min
    *** reqs: xGnai3 [omission]
    *** source: 19401591-Fig-3b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (tnr)
    *** cells: mEFs in BMLS
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-4c

Gnai1-- and/or Gnai3--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (15 min)
    *** cells: mEFs in BMLS
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-1a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ 15+ 30+ min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-1b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ 15+ 30+ min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-2d
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5++ 15++ min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-2c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 10 20+ min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** reqs: Gnai1 and/or Gnai3 [RNAi]
    *** source: 19401591-Fig-3a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 20+ min
    *** inhibited by: xGnai1(G202T)"DN" + xGnai3(G202T)"DN" [addition]
    *** source: 19401591-Fig-3c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 20+ min
    *** partially reqs: Gnai3 [KO]
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-1c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gnai1~null><Gnai3~null><xGnai1> in BMLS
    *** times: 0 10++ 20+ min
    *** reqs: xGnai1 [omission]
    *** source: 19401591-Fig-3b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Gnai1~null><Gnai3~null><xGnai3> in BMLS
    *** times: 0 10++ 20+ min
    *** reqs: xGnai3 [omission]
    *** source: 19401591-Fig-3b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (tnr)
    *** cells: mEFs in BMLS
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-4c
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (15 min)
    *** cells: mEFs in BMLS
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-1a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ 15 30 min
    *** reqs: Gnai1 and/or Gnai3 [KO]
    *** source: 19401591-Fig-1b

not Gnai2--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+ 5+ 15+ 30+ min
    *** does not req: Gnai2 [KO]
    *** source: 19401591-Fig-1d

Jip1--

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** partially reqs: Jip1 [RNAi] no-bkg-control
    *** source: 18285454-Fig-7d

not Mek12s--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15+ min
    *** unaffected by: PD98059 [addition]
    *** source: 16984645-Fig-4c

not Mlk3--

  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Egf (10 min)
    *** cells: CCD18CO in BMLS
    *** does not req: Mlk3 [RNAi]
    *** source: 15258589-Fig-2a

not Mras--

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: mAstrocytes in BMLS
    *** times: 0 2++ 5++ 15++ 40+ 90 min
    *** does not req: Mras [KO]
    *** source: 16980617-Fig-7a

inhibited by Npr12--

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (10 min)
    *** cells: HT1080 in BMLS
    *** comment: cells were refed at the same time as Egf addition
    *** inhibited by: xNprl2 [addition]
    *** unaffected by: xNprl2(134-380)"dN133" [addition]
    *** source: 18616680-Fig-4c

not Phb--

  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMLS
    *** times: 0 2++ 10+ min
    *** does not req: Phb [RNAi]
    *** source: 16041367-Fig-3a

Pi3k-- ???

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 1+ 5+ 10+ 20++ min
    *** unaffected by: xPik3c2b [addition]
    *** source: 16775008-Fig-4d
  *** xAkt1[tAb]IP IVKA(HistH2B)[32P-ATP] is increased irt Egf (5 min)
    *** cells: VERO in BMLS
    *** inhibited by: xPik3r?(mnr)"DN" [addition] no-bkg-control
    *** partially inhibited by: xPik3ca(K802R)"KD" [addition] no-bkg-control
    *** partially inhibited by: xPik3cb(K805R)"KD" [addition] no-bkg-control
    *** source: 11134009-Fig-2b

Ppp2r1a-- ???

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: PC63 in BMS
    *** times: 0 5+ 15 30 min
    *** bkg reqs: Ppp2r1a [RNAi]
    *** source: 16129692-Fig-2a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: PC63<xEgfR> in BMS
    *** times: 0 5+ 15+ 30+ min
    *** comment: sustained signal (15-30 min) reqs Ppp2r1a [RNAi]
    *** source: 16129692-Fig-4

Ptk6--  ???

  *** Akt1[tAb]IP phos(S473)[phosAb] is increased irt Egf (10 min)
    *** cells: COS1 in BMLS
    *** unaffected by: xPtk6 [addition]
    *** unaffected by: xPtk6(Y447F) [substitution]
    *** unaffected by: xPtk6(K219M)"KD" [addition]
    *** source: 15539407-Fig-5c
  *** Akt1[tAb]IP phos(T308)[phosAb] is increased irt Egf (10 min)
    *** cells: COS1 in BMLS
    *** unaffected by: xPtk6 [addition]
    *** unaffected by: xPtk6(Y447F) [substitution]
    *** unaffected by: xPtk6(K219M)"KD" [addition]
    *** source: 15539407-Fig-5c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: BPH1 in BMLS
    *** partially reqs: Ptk6 [RNAi]
    *** source: 20606012-Fig-8a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: SYF<Src~null><Yes1~null><Fyn~null> in BMLS
    *** enhanced by: xPtk6 [addition]
    *** source: 20606012-Fig-6b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: SYF<Src~null><Yes1~null><Fyn~null> in BMLS
    *** unaffected by: xPtk6 [addition]
    *** comment: author calls this an enhancement
    *** source: 20606012-Fig-7c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: SYF<Src~null><Yes1~null><Fyn~null> in BMLS
    *** unaffected by: xPtk6 [addition]
    *** unaffected by: xPtk6(Y447F)"CA" [addition]
    *** unaffected by: xPtk6(K219M)"KD" [addition]
    *** comment: author calls this an enhancement with FL and CA but not KD
    *** source: 20606012-Fig-6b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: SYF<Src~null><Yes1~null><Fyn~null> in BMLS
    *** times: 0 5++ 5+ min
    *** enhanced by: xPtk6 [addition]
    *** source: 20606012-Fig-6a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (5 min)
    *** cells: BPH1 in BMLS
    *** partially reqs: Ptk6 [RNAi]
    *** source: 20606012-Fig-8a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (5 min)
    *** cells: SYF<Src~null><Yes1~null><Fyn~null> in BMLS
    *** enhanced by: xPtk6 [addition]
    *** source: 20606012-Fig-6b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (5 min)
    *** cells: SYF<Src~null><Yes1~null><Fyn~null> in BMLS
    *** unaffected by: xPtk6 [addition]
    *** comment: author calls this an enhancement
    *** source: 20606012-Fig-7c
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (5 min)
    *** cells: SYF<Src~null><Yes1~null><Fyn~null> in BMLS
    *** unaffected by: xPtk6 [addition]
    *** unaffected by: xPtk6(Y447F)"CA" [addition]
    *** unaffected by: xPtk6(K219M)"KD" [addition]
    *** comment: author calls this an enhancement with FL and CA but not KD
    *** source: 20606012-Fig-6b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: SYF<Src~null><Yes1~null><Fyn~null> in BMLS
    *** times: 0 5++ 5+ min
    *** enhanced by: xPtk6 [addition]
    *** source: 20606012-Fig-6a




Rictor--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** reqs: Rictor [KO]
    *** source: 21464307-Fig-S4a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** reqs: Rictor [KO]
    *** source: 21464307-Fig-S4a

Tbk1-- can be substituted with Rictor or Ikke

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs<Rictor~null> in BMLS
    *** partially reqs: Tbk1 [RNAi]
    *** source: 21464307-Fig-4c
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs<Rictor~null> in BMLS
    *** partially reqs: Tbk1 [RNAi]
    *** source: 21464307-Fig-4c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5++ 15+ 30 min
    *** partially reqs: Ikke and/or Tbk1 [KO]
    *** source: 21464307-Fig-2a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5++ 15+ 30 min
    *** does not req: Ikke or Tbk1 [KO]
    *** source: 21464307-Fig-2a

not Rac1--

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (tnr)
    *** cells: COS7 in BMLS
    *** unaffected by: xRac1(T17N)"DN" [addition]
    *** source: 15034142-Fig-5e

RalGds--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: MCF10A in BMLS
    *** times: 0 5++ 10++ 30+ min
    *** partially reqs: RalGds [RNAi]
    *** source: 18285454-Fig-1c
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: MCF10A in BMLS
    *** times: 0 5+ 10+ 30 min
    *** partially reqs: RalGds [RNAi]
    *** source: 18285454-Fig-1c

Rheb--  ???

  *** xAkt1[phosAb] phos(S473)[phosAb] is increased irt Egf (10 min)
    *** cells: HEK293E<xAkt1> in BMLS
    *** partially inhibited by: xRheb [addition]
    *** source: 12906785-Fig-1b
  *** xAkt1[tAb]IP IVKA(Gsk3b)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293E<xAkt1> in BMLS
    *** unaffected by: xRheb [addition]
    *** source: 12906785-Fig-1b

Sin1-- for phos(S473)

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (15 min)
    *** cells: mEFs in BMLS
    *** reqs: Sin1 [KO]
    *** source: 16962653-Fig-5b

  not for phos(T308)

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (15 min)
    *** cells: mEFs in BMLS
    *** does not req: Sin1 [KO]
    *** source: 16962653-Fig-5b

not Shoc2--

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (8 min)
    *** cells: BT549 in BMS
    *** does not req: Shoc2 [RNAi]
    *** source: 16630891-Fig-5b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (8 min)
    *** cells: HBL100 in BMS
    *** does not req: Shoc2 [RNAi]
    *** source: 16630891-Fig-5b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (8 min)
    *** cells: MCF10A in BMS
    *** does not req: Shoc2 [RNAi]
    *** source: 16630891-Fig-5b

Chemicals

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS
    *** times: 0 7+ 15+ min
    *** unaffected by: Rapamycin [addition]
    *** source: 16984645-Fig-5
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15+ min
    *** unaffected by: Rapamycin [addition]
    *** source: 16984645-Fig-4a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS
    *** times: 0 7+ 15 min
    *** inhibited by: Rapamycin [addition]
    *** source: 16984645-Fig-5
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (tnr)
    *** cells: COS7 in BMLS
    *** unaffected by: STI571 [addition] no-bkg-control
    *** source: 15039778-Fig-4g
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (tnr)
    *** cells: COS7 in BMLS
    *** inhibited by: Wortmannin [addition] no-bkg-control
    *** source: 15039778-Fig-4g
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: COS in BMS
    *** times: 0 2+ 5+ 10+ 20+ 60+ min
    *** inhibited by: Wortmannin [addition]
    *** inhibited by: LY294002 [addition]
    *** source: 11259591-Fig-1a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0+ 1++ 5++ 10+ min
    *** bkg inhibited by: Wortmannin [addition]
    *** source: 16687399-Fig-2a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (tnr)
    *** cells: COS7 in BMLS
    *** inhibited by: Wortmannin [addition]
    *** source: 15034142-Fig-5e
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (tnr)
    *** cells: COS7 in BMS
    *** inhibited by: LY294002 [addition] no-bkg-control
    *** source: 12454019-Fig-5d
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS
    *** times: 0 7+ 15+ min
    *** inhibited by: LY294002 [addition]
    *** source: 16984645-Fig-5
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15+ min
    *** inhibited by: LY294002 [addition]
    *** source: 16984645-Fig-4c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0 7+ 15+ min
    *** inhibited by: LY294002 [addition]
    *** source: 16984645-Fig-4a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (30 min)
    *** cells: NR6<xEgfR> in BMLS
    *** inhibited by: LY294002 [addition] no-bkg-control
    *** source: 14747473-Fig-6d
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS
    *** times: 0 7+ 15 min
    *** inhibited by: LY294002 [addition]
    *** source: 16984645-Fig-5
  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Egf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: U0126 [addition]
    *** source: 11896055(D)
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (tnr)
    *** cells: COS7 in BMLS
    *** unaffected by: CytochalasinD [addition]
    *** unaffected by: LatrunculinB [addition]
    *** source: 15034142-Fig-5e
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: COS7 in BMLS
    *** inhibited by: LY294002 [pretreatment]
    *** source: 10330169-Fig-5
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: T47D in BMLS
    *** times: 0 5+ 15+ 30++ 60+ min
    *** bkg inhibited by: PP1 [addition]
    *** source: 11994282-Fig-7
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: rHepatocytes in BMLS
    *** inhibited by: PP1 [addition] no-bkg-control
    *** source: 12464621-Fig-3b
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Egf (times)
    *** cells: HC11 in BMLS "containing Ins"
    *** times: 0+ 7+ 15+ min
    *** unaffected by: LY294002 [addition]
    *** unaffected by: PD98059 [addition]
    *** source: 16984645-Fig-4c
  *** Akt1[phosAb] phos(TEY)[phosAb] is increased irt Egf (30 min)
    *** cells: HEK293<xTsc2> in BMLS
    *** unaffected by: U0126 [pretreatment] no-bkg-control
    *** inhibited by: Wortmannin [pretreatment] no-bkg-control
    *** source: 15851026-Fig-1b
  *** xAkt1[phosAb] phos(S473)[phosAb] is increased irt Egf (10 min)
    *** cells: HEK293E<xAkt1> in BMLS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 12906785-Fig-1b
  *** xAkt1[tAb]IP IVKA(Gsk3b)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293E<xAkt1> in BMLS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 12906785-Fig-1b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (10 min)
    *** cells: COS1<xPtk6> in BMLS
    *** inhibited by: LY294002 [cotreatment]
    *** source: 15539407-Fig-2d
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 20+ min
    *** unaffected by: PTX [pretreatment]
    *** source: 19401591-Fig-3e

No extras

  *** xAkt1[tAb]IP IVKA(HistH2B)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS7<xAkt1> in BMLS
    *** source: 10330169-Fig-3d
  *** xAkt1[tAb]IP IVKA(HistH2B)[32P-ATP] is increased irt Egf (times)
    *** cells: MDCK<xAkt1> in BMLS
    *** times: 0+ 5++ 15+ 30+ 60 120 180 240 min
    *** source: 9668053-Fig-1b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: MDAMB361 in BMLS
    *** times: 0 5+ 10+ 20+ 30+ 45+ 60+ min
    *** source: 11297548(D)
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0+ 1++ 5++ 10++ min
    *** source: 16687399-Fig-8e
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: PC6 in BMLS
    *** source: 15632082-Fig-1c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** source: 16687399-Fig-3a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (5 min)
    *** cells: HEK293 in BMLS
    *** times: 0+ 1+ 3++ 5++ 10+ 30+ min
    *** source: 16687399-Fig-3b
  *** xAkt1[phosAb] phos(T308)[phosAb] is increased irt Egf (5 min)
    *** cells: COS7<xRalGds> in BMLS
    *** source: 18285454-Fig-7e
  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Egf (15 min)
    *** cells: T47D in BMS
    *** source: 11196191(D)
  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Egf (5 min)
    *** cells: U87MG in BMLS
    *** source: 14701753-Fig-2a,2b
  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Egf (5 min)
    *** cells: PC12 in BMLS
    *** source: 11733534-Fig-2c
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Egf (10 min)
    *** cells: HEK293E in BMS
    *** source: 12906785-Fig-1b
  *** Akts[phosAb] phos(sitenr)[phosAb] is increased irt Egf (times)
    *** cells: COS7 in BMLS
    *** times: 0 15+ 30+ 120+ min
    *** source: 16525023-Fig-6a

Conditions causing no increase

  *** Akt1[phosAb] phos(S473)[phosAb] is detectable-but unchanged irt Egf (15 min)
    *** cells: hESKs<xEgfR> in BMLS "Egf and BPE starved"
    *** source: 15284024-Fig-2
  *** Akt1[phosAb] phos(T308)[phosAb] is unchanged irt Egf (15 min)
    *** cells: HEK293<xTbc1d4> in BMLS
    *** source: 17617058-Fig-6
  *** xAkt1[tAb]IP phos(S473)[phosAb] is detectable-but unchanged irt Egf (10 min)
    *** cells: COS1<xAkt1> in BMLS
    *** source: 15539407-Fig-3b


==============================================
Association with other members of EgfR complex
==============================================

Freud1--

  *** Akt1[Ab] copptby[WB] Freud1[Ab] is increased irt Egf (10 min)
    *** cells: A431 in BMLS
    *** source: 18662999-Fig-5c
  *** Akt1[Ab] copptby[WB] xFreud1[tAb] is increased irt Egf (10 min)
    *** cells: HT1080 in BMLS
    *** inhibited by: AG1478 [addition]
    *** source: 18662999-Fig-5d

Plcg1--

  *** xAkt1[tAb] copptby[WB] Plcg1[Ab] is increased irt Egf (30 min)
    *** cells: COS7<xAkt1> in BMLS
    *** source: 16525023-Fig-4a
  *** Akts[Ab] copptby[WB] Plcg1[Ab] is increased irt Egf (30 min)
    *** cells: COS7 in BMLS
    *** source: 16525023-Fig-2a



================
Missing Evidence
================

coprecipitation of Akt1 with EgfR in response to Egf

===========
Other Stuff
===========

  *** Akt1[phosAb] phos(sitenr)[phosAb] is detectable
    *** cells: MDAMB468 in BMLS "treated with Egf for 5 hr"
    *** inhibited by: Gefitinib [pretreatment]
    *** inhibited by: AG1478 [pretreatment]
    *** unaffected by: AG490 [pretreatment]
    *** unaffected by: LY294002 [pretreatment]
    *** unaffected by: U0126 [pretreatment]
    *** source: 17909010-Fig-3a
