Assume for now that Cynnb1 in cytosolic fraction and prot-exp mean the same thing
======================================================
Ctnnb1 to cytosolic fraction and increased it prot-exp
======================================================

inhibited itpo xAxin1--

  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: Lcells in BMS
    *** inhibited by: xAxin1 [addition]
    *** source: 10023673-Fig-2a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (times)
    *** cells: Lcells in BMS
    *** times: 0+ 30++ 60+++ 180++++ 300++++ min
    *** inhibited by: xAxin1 [addition]
    *** source: 10023673-Fig-2b

not Clathrin--

  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (1 hr)
    *** cells: HELAS3 in BMS
    *** partially inhibited by: Wnt5a [cotreatment]
    *** unaffected by: Clathrin [RNAi]
    *** source: 19910923-Fig-6e

not Csnk1a1--

  *** Ctnnb1[Ab] infraction(cytosolic)[WB] is increased irt Wnt3a (1 hr)
    *** cells: HELAS3 in BMS
    *** does not req: Csnk1a1 [RNAi]
    *** source: 12556519-Fig-9a

can use Csnk1e--

  *** Ctnnb1[Ab] infraction(cytosolic)[WB] is increased irt Wnt3a (1 hr)
    *** cells: HELAS3 in BMS
    *** partially reqs: Csnk1e [RNAi]
    *** source: 12556519-Fig-9a

uses Dvl1-- and/or Dvl2-- and/or Dvl3--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** reqs: Dvl1 and/or Dvl2 and/or Dvl3 [RNAi]
    *** source: 18772438-Fig-S1a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (6 hr)
    *** cells: Lcells in BMS
    *** unaffected by: xDvl1(mnr)"delPDZ" [addition]
    *** source: 10196136-Fig-4b

uses Fzd2-- and/or Fzd4-- and/or Fzd5--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** reqs: Fzd2 and/or Fzd4 and/or Fzd5 [RNAi]
    *** source: 18772438-Fig-S5b

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** partially reqs: Fzd2 [RNAi]
    *** source: 18772438-Fig-S5b
  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** partially reqs: Fzd4 [RNAi]
    *** source: 18772438-Fig-S5b
  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** partially reqs: Fzd5 [RNAi]
    *** source: 18772438-Fig-S5b

  *** Ctnnb1[Ab] infraction(cytosolic)[WB] is increased irt Wnt3a (1 hr)
    *** cells: Lcells in BMS
    *** reqs: Fzd2 and/or Fzd7 [RNAi]
    *** source: 18077588-Fig-1b

not Fzd3--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** does not req: Fzd3 [RNAi]
    *** source: 18772438-Fig-S5b

not Fzd6--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** does not req: Fzd6 [RNAi]
    *** source: 18772438-Fig-S5b

uses Lrp5-- and/or Lrp6--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** reqs: Lrp5 and/or Lrp6 [RNAi]
    *** source: 18772438-Fig-S1a
  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** reqs: Lrp5 and/or Lrp6 [RNAi]
    *** source: 18772438-Fig-S5b

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** partially reqs: Lrp5 [RNAi]
    *** source: 18772438-Fig-S5b
  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** partially reqs: Lrp6 [RNAi]
    *** source: 18772438-Fig-S5b

uses Pi4k2a--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** reqs: Pi4k2a [RNAi]
    *** comment: requirement for Pi4k2a can be partially overridden by pretreatment with PI45P2
    *** source: 18772438-Fig-S3a
  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** reqs: Pi4k2a [RNAi]
    *** source: 18772438-Fig-S1a
  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** reqs: Pi4k2a [RNAi]
    *** source: 18772438-Fig-S1c

not Pi4k2b--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** does not req: Pi4k2b [RNAi]
    *** source: 18772438-Fig-S1a

not Pi4ka--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** does not req: Pi4ka [RNAi]
    *** source: 18772438-Fig-S1a

not Pi4kb--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** does not req: Pi4kb [RNAi]
    *** source: 18772438-Fig-S1a

not Pip5k1a--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** does not req: Pip5k1a [RNAi]
    *** source: 18772438-Fig-S1a
  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** does not req: Pip5k1a [RNAi]
    *** source: 18772438-Fig-S1c

uses Pip5k1b--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** partially reqs: Pip5k1b [RNAi]
    *** source: 18772438-Fig-S1a
  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** reqs: Pip5k1b [RNAi]
    *** comment: requirement for Pi5k1b can be partially overridden by pretreatment with PI45P2
    *** source: 18772438-Fig-S3b
  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** reqs: Pip5k1b [RNAi]
    *** source: 18772438-Fig-S1a

not Pip5k1c--

  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** does not req: Pip5k1c [RNAi]
    *** source: 18772438-Fig-S1a
  *** Ctnnb1[Ab] infraction(cytosolic)[ELISA] is increased irt Wnt3a (3 hr)
    *** cells: HEK293T in BMS
    *** does not req: Pip5k1c [RNAi]
    *** source: 18772438-Fig-S1c

not Ror1-- or Ror2--

  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: HELAS3 in BMS
    *** does not req: Ror1 [RNAi]
    *** source: 19910923-Fig-6a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: HELAS3 in BMS
    *** does not req: Ror2 [RNAi]
    *** source: 19910923-Fig-6a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: HELAS3 in BMS
    *** does not req: Ror1 or Ror2 [RNAi]
    *** source: 19910923-Fig-6a

Rgs19--  ???

  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (times)
    *** cells: F9<xFzd1> in BMS "lysates precleared with ConA-ppt"
    *** times: 0+ 2+ 4+ hr
    *** reqs: Rgs19 [RNAi]
    *** source: 17855383-Fig-8c

===========================
Effect of Wnt5a cotreatment
===========================

  *** Ctnnb1[Ab] infraction(cytosolic)[WB] is increased irt Wnt3a (3 hr)
    *** cells: HEK293 in BMS
    *** unaffected by: Wnt5a [cotreatment]
    *** source: 16602827-Fig-2a
  *** Ctnnb1[Ab] infraction(cytosolic)[WB] is increased irt Wnt3a (3 hr)
    *** cells: HEK293 in BMS
    *** unaffected by: Wnt5a [cotreatment]
    *** source: 16602827-Fig-4a
  *** Ctnnb1[Ab] infraction(cytosolic)[WB] is increased irt Wnt3a (3 hr)
    *** cells: HEK293<xFzd4> in BMS
    *** unaffected by: Wnt5a [cotreatment]
    *** source: 16602827-Fig-4a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: C3H10T12 in BMS
    *** inhibited by: Wnt5a [cotreatment]
    *** comment: Ctnnb1 prot decreases after removal of Wnt3a - this process is not affected by addition of Wnt5a
    *** source: 19910923-Fig-4c
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: C3H10T12 in BMS
    *** inhibited by: Wnt5a [cotreatment]
    *** source: 19910923-Fig-4b
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: C3H10T12 in BMS
    *** inhibited by: Wnt5a [cotreatment]
    *** source: 19910923-Fig-4d
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: CHO in BMS
    *** inhibited by: Wnt5a [cotreatment]
    *** source: 19910923-Fig-4b
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: HELAS3 in BMS
    *** inhibited by: Wnt5a [cotreatment]
    *** source: 19910923-Fig-4b
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: HELAS3 in BMS
    *** inhibited by: Wnt5a [cotreatment]
    *** source: 19910923-Fig-6a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: Lcells in BMS
    *** inhibited by: Wnt5a [cotreatment]
    *** source: 19910923-Fig-4b
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: MKN1 in BMS
    *** inhibited by: Wnt5a [cotreatment]
    *** source: 19910923-Fig-4b
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (3 hr)
    *** cells: NIH3T3 in BMS
    *** inhibited by: Wnt5a [cotreatment]
    *** source: 19910923-Fig-4b

Inhibitors

  *** Ctnnb1[Ab] infraction(cytosolic)[WB] is increased irt Wnt3a (1 hr)
    *** cells: HEK293T<xLrp6> in BMS
    *** inhibited by: xShisa2 [addition]
    *** source: 18077588-Fig-1a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (1 hr)
    *** cells: HELAS3 in BMS
    *** partially inhibited by: Wnt5a [cotreatment]
    *** unaffected by: xEpn1(mnr)"EnthDomain" [addition]
    *** source: 19910923-Fig-6e

Chemicals

  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (1 hr)
    *** cells: L929 in BMS
    *** unaffected by: CTX [pretreatment]
    *** source: 16303557-Fig-6b
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (2 hr)
    *** cells: HEK293T in BMS
    *** unaffected by: xWtx [addition]
    *** source: 17510365-Fig-2b


No extras

  *** Ctnnb1[Ab] infraction(cytosolic)[WB] is increased irt Wnt3a (2 hr)
    *** cells: 3T3L1 in BMS
    *** source: 16303557-Fig-2b
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (1 hr)
    *** cells: L929 in BMS
    *** source: 16303557-Fig-1b
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (2 hr)
    *** cells: Lcells in BMS
    *** source: 10421629-Fig-1b
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (5 hr)
    *** cells: JURKATT in BMS
    *** source: 12000790-Fig-6a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (5 hr)
    *** cells: Lcells in BMS
    *** source: 12000790-Fig-6a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (6 hr)
    *** cells: Lcells in BMS
    *** source: 10196136-Fig-4a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (30 min)
    *** cells: Lcells in BMS
    *** source: 14722104-Fig-1c
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (times)
    *** cells: HEK293 in BMLS
    *** times: 0+ 2++ 6++ 24++ hr
    *** source: 19131971-Fig-1e
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (times)
    *** cells: L929 in BMS
    *** times: 0+ 30+ 60++ 120+++ 180++++ min
    *** source: 16303557-Fig-1a
  *** Ctnnb1[Ab] prot-exp[WB] is increased irt Wnt3a (times)
    *** cells: F9<xFzd1> in BMS "lysates precleared with ConA-ppt"
    *** times: 0+ 1+ 2+ 3+ 4+ 5+ hr
    *** bkg inhibited by: xRgs19 [addition]
    *** source: 17855383-Fig-5a



Contradictions

  *** Ctnnb1[Ab] prot-exp[WB] is unchanged irt Wnt3a (1 hr)
    *** cells: L929 in BMS
    *** unaffected by: CTX [pretreatment]
    *** source: 16303557-Fig-6b
  *** Ctnnb1[Ab] prot-exp[WB] is unchanged irt Wnt3a (2 hr)
    *** cells: 3T3L1 in BMS
    *** source: 16303557-Fig-1a
