================================================================
Mlk3 oligomerization requires L410 in the leucine zipper region
================================================================

  *** xMlk3[tAb] oligomerization[2TagPpt] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(L410P)"oligo-mut" [substitution]
    *** source: 10862766(D)
  *** xMlk3[tAb] oligomerization[2TagPpt] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(del(403-462))"leucine-zipper-mutant" [substitution]
    *** source: 9829970-Fig-4

================================================================
Mlk3 oligomerization does not require Cterm or kinase actvity
================================================================

  *** xMlk3[tAb] oligomerization[2TagPpt] is detectable
    *** cells: HEK293 in BMS
    *** source: 9829970-Fig-2
  *** xMlk3[tAb]IP oligomerization[NativeWB] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMlk3(1-591)"Cterm-mutant" [substitution]
    *** source: 9829970-Fig-3
  *** xMlk3[tAb] oligomerization[2TagPpt] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMlk3(K144R)"KD" [substitution]
    *** source: 9829970-Fig-6

======================================
Mlk3 autophosphorylation requires K144
======================================

  *** xMlk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(K144A)"KD" [substitution]
    *** source: 10232608-Fig-5a
  *** xMlk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(K144A)"KD" [substitution]
    *** source: 10232608-Fig-5b
  *** xMlk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(K144A)"KD" [substitution]
    *** source: 10232608-Fig-5c
  *** xMlk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(K144A)"KD" [substitution]
    *** source: 10232608-Fig-5d
  *** xMlk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HELA in BMS
    *** inhibited by: xMlk3(K144R) [substitution]
    *** source: 11053428-Fig-1b
  *** xMlk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(K144A) [substitution]
    *** source: 8195146-Fig-7a

======================================
Mlk3 autophosphorylation requires E164
======================================

  *** xMlk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(E164A) [substitution]
    *** source: 8195146-Fig-7a

=============================================
Mlk3 kinase activity requires oligomerization
=============================================

  *** xMlk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(del(403-462))"leucine-zipper-mutant" [substitution]
    *** source: 9829970-Fig-7
  *** xMlk3[Ab]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(L410P)"oligo-mut" [substitution]
    *** source: 10862766(D)
  *** xMlk3[Ab]IP IVKA(histones)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xMlk3(L410P)"oligo-mut" [substitution]
    *** source: 10862766(D)

==============================================
Mlk3 autphosphorylates on Serine and Threonine
==============================================

  *** xMlk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** comment: xMlk3 is phosed on Serine and Threonine [PAA]
    *** source: 8195146-Fig-7c

=================================================
Mlk3 autphosphorylates on T277 and S281 not T278
=================================================

  *** xMlk3[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HELA in BMS
    *** inhibited by: xMlk3(T277A) [substitution]
    *** unaffected by: xMlk3(T278A) [substitution]
    *** partially inhibited by: xMlk3(S281A) [substitution]
    *** source: 11053428-Fig-1b

=====
coppt
=====

  *** Mlk3[Ab] copptby[WB] Braf[Ab] is detectable
    *** cells: CCD18CO in BMLS
    *** unaffected by: Egf (10 min) [stim]
    *** source: 15258589-Fig-3e
  *** Mlk3[Ab] copptby[WB] Jip1[Ab] is detectable
    *** cells: PC12 in BMS "neuronally differentiated"
    *** source: 16571722-Fig-2b
  *** Mlk3[Ab] copptby[WB] Merlin[Ab] is detectable
    *** cells: F4328 in BMLS
    *** unaffected by: Egf (10 min) [stim]
    *** source: 16537381-Fig-4a
  *** xMlk3[tAb] copptby[WB] xMkk3[tAb] is undetectable
    *** cells: COS1 in BMS
    *** source: 9003778-Fig-6a
  *** xMlk3[tAb] copptby[WB] xMkk4[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 9003778-Fig-6a
  *** xMlk3[tAb] copptby[WB] xPosh[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 12514131-Fig-3a
  *** xMlk3[tAb] copptby[WB] xPosh[tAb] is detectable
    *** cells: HEK293 in BMS
    *** enhanced by: xPosh(W489A) [substitution]
    *** enhanced by: LY294002 [addition]
    *** source: 14504284(D)
