=====================================================
IL2 binding to IL2R requires ILRa and IL2Rb or IL2Rg
=====================================================

  *** rIL2[125I] receptor-binding[RIA] is detectable
    *** cells: L929<xIL2Ra><xIL2Rb><xIL2Rg> in BMS
    *** partially reqs: xIL2Ra [omission]
    *** partially reqs: xIL2Rg [omission]
    *** reqs: xIL2Ra or xIL2Rb [omission]
    *** reqs: xIL2Ra or xIL2Rg [omission]
    *** partially reqs: xIL2Rb or xIL2Rg [omission]
    *** source: 1631559-Fig-4
  *** xIL2Rb[125I] boundby[Xlink] rIL2[125I] is detectable
    *** cells: BAFB03<xIL2Rb> in BMS
    *** reqs: xIL2Rb [omission]
    *** unaffected by: xIL2Rb(1-404)"H-mutant" [substitution]
    *** unaffected by: xIL2Rb(del(338-409))"A-mutant" [substitution]
    *** unaffected by: xIL2Rb(del(292-349))"S-mutant" [substitution]
    *** unaffected by: xIL2Rb(1-281)"ST-mutant" [substitution]
    *** source: 2590941-Fig-4
  *** xIL2Rb[125I] boundby[Xlink] rIL2[125I] is detectable
    *** cells: COS7<xIL2Ra><xIL2Rb> in BMS
    *** unaffected by: xIL2Rb(Y364F/Y381F/Y383F/Y387F/Y418F/Y536F)"FFFFFF" [substitution]
    *** source: 7665592-Fig-1d

====================================
Formation of IL2Rabg complex irt IL2
====================================

  *** IL2Rg[125I] copptby[WB] xIL2Rb[Ab] is increased irt IL2 (tnr)
    *** cells: MOLT4 in BMS
    *** source: 1631559-Fig-1

===========================
IL2Rb Yphos in response IL2
===========================

  *** xIL2Rb(Y381F/Y383F/Y387F)[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: 32D<IL2Rb~null> in BMS "IL3-deprived"
    *** bkg inhibited by: xIL2Rb(Y364F/Y381F/Y383F/Y387F/Y418F/Y536F)"FFFFFF" [substitution]
    *** source: 8700888-Fig-2a
  *** IL2Rb[Ab]IP IVKA(HistH1)[32P-ATP] is decreased irt IL2 (times)
    *** cells: hPHAblasts in BMS
    *** times: 0+ 2+ 5 15 min
    *** source: 1639773-Fig-2
  *** IL2Rb[Ab]IP IVKA(HistH1)[32P-ATP] is decreased irt IL2 (times)
    *** cells: hPHAblasts in BMS
    *** times: 0++ 2+ 5 min
    *** inhibited by: Genistein [pretreatment]
    *** source: 1639773-Fig-3a
  *** IL2Rb[Ab]IP phos[32Pi] is increased irt IL2 (5 min)
    *** cells: ILTMAT in BMS "IL2-deprived"
    *** source: 2106566-Fig-3
  *** IL2Rb[Ab]IP phos[32Pi] is increased irt IL2 (10 min)
    *** cells: MT2 in BMS
    *** comment: phosphorylation occurred on Tyr [PAA]
    *** source: 1618313-Fig-4
  *** IL2Rb[Ab]IP phos[32Pi] is increased irt IL2 (times)
    *** cells: ILTMAT in BMS "IL2-deprived"
    *** times: 0+ 1++ 5++ 15++ 30++ min
    *** source: 2106566-Fig-1
  *** xIL2Rb[Ab]IP phos[32Pi] is increased irt IL2 (times)
    *** cells: MOLT4 in BMS
    *** times: 0 1+ 5++ 15++ 30++ min
    *** comment: phosphorylation occurred on Tyr and Ser [PAA]
    *** source: 1618313-Fig-1
  *** xIL2Rb[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: BAFB03<xIL2Rb> in BMS "IL3-deprived"
    *** partially inhibited by: xIL2Rb(del(266-323)) [substitution]
    *** unaffected by: xIL2Rb(del(312-383)) [substitution]
    *** unaffected by: xIL2Rb(Y335F/Y358F) [substitution]
    *** source: 8643566-Fig-2
  *** IL2Rb[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: CTLL20 in BMS "IL2-deprived"
    *** unaffected by: xShc1 [addition]
    *** unaffected by: xShc1(233-471) [addition]
    *** source: 8643566-Fig-4c
  *** xIL2Rb[Ab]IP Yphos[pYAb] is increased irt IL2 (5 min)
    *** cells: HEK293T<xIL2Ra><xIL2Rg><xJak3><xGab2> in BMS
    *** partially inhibited by: xIL2Rb(Y338F) [substitution]
    *** source: 10849428-Fig-4a
  *** xIL2Rb[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: BAF3<IL2Rb~null> in BMS
    *** unaffected by: xSocs3 [addition]
    *** source: 10373548-Fig-4c
  *** IL2Rb[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: KIT225 in BMLS "IL2-deprived"
    *** source: 10373548-Fig-3a
  *** xIL2Rb[Ab]IP Yphos[pYAb] is increased irt IL2 (1 hr)
    *** cells: BAF3<IL2Rb~null><xIL2Rb> in BMS
    *** source: 10514520-Fig-2b
  *** IL2Rb[Ab]IP Yphos[pYAb] is increased irt IL2 (times)
    *** cells: CTLL2 in BMS "IL2-deprived"
    *** times: 0 5+ 15+ min
    *** unaffected by: xDusp5 [addition]
    *** unaffected by: xDusp5(C263S)"CIA" [addition]
    *** source: 12435740-Fig-6c

  *** xIL2Rb[Ab] copptby[WB] xJak1[Ab] is detectable
    *** cells: COS7 in BMS
    *** source: 7973658-Fig-3a
  *** xIL2Rg[Ab] copptby[WB] xJak1[Ab] is undetectable
    *** cells: COS7 in BMS
    *** source: 7973658-Fig-3b
