==================
Direct Association
==================

  *** rGsk3b[Ab] boundby[WB] rAxin1[MalBP] is detectable
    *** cells: none
    *** unaffected by: rCtnnb1 [addition]
    *** source: 10698523-Fig-3c
  *** rGsk3b[Ab] boundby[WB] rAxin1[MalBP] is detectable
    *** cells: none
    *** unaffected by: rApc(1211-2075) [addition]
    *** source: 10698523-Fig-2d
  *** rAxin1(298-506)[tAb] boundby[WB] rGsk3b[GST] is detectable
    *** cells: none
    *** comment: unaffected by ATP in the binding buffer
    *** source: 9482734-Fig-7a
  *** rAxin1(298-506)[tAb] boundby[WB] rGsk3b[GST] is detectable
    *** cells: none
    *** unaffected by: rAxin1(353-437) [substitution]
    *** inhibited by: rAxin1(437-506) [substitution]
    *** comment: numbering refers to rat Axin1
    *** source: 9482734-Fig-8b

unaffected by Dvl1

  *** rGsk3b[GST] boundby[WB] rAxin1[MalBP] is detectable
    *** cells: none
    *** unaffected by: rDvl1 [addition]
    *** source: 10330181-Fig-5d

===========================
Axin1 Gsk3b coprecipitation
===========================

Axin1 required feature

  *** xAxin1[tAb] copptby[WB] xGsk3b[tAb] is detectable
    *** cells: HEK293T<xAxin1> in BMS
    *** inhibited by: xAxin1(L525P)"Gsk3b-binding-mut" [substitution]
    *** source: 12000790-Fig-3b
  *** Gsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293<xAxin1><xMyc> in BMS
    *** inhibited by: xAxin1(del(340-418))"delEx4-5" [substitution]
    *** inhibited by: xAxin1(del(372-418))"delEx5" [substitution]
    *** source: 19131971-Fig-7b
  *** Gsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293<xAxin1><xMyc> in BMS
    *** inhibited by: xAxin1(del(340-418))"delEx4-8" [substitution]
    *** source: 19131971-Fig-6d
  *** Gsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293<xAxin1><xMyc> in BMS
    *** unaffected by: xAxin1(1-594)"Ex2-6" [substitution]
    *** inhibited by: xAxin1(595-862)"Ex6-11" [substitution]
    *** inhibited by: xAxin1(del(340-764))"delEx4-9" [substitution]
    *** source: 19131971-Fig-6b
  *** Gsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293<xAxin1><xMyc> in BMS
    *** unaffected by: xAxin1(1-594)"Ex2-6" [substitution]
    *** unaffected by: xAxin1(1-651)"Ex2-7" [substitution]
    *** unaffected by: xAxin1(1-728)"Ex2-8" [substitution]
    *** unaffected by: xAxin1(1-764)"Ex2-9" [substitution]
    *** source: 19131971-Fig-6c
  *** xGsk3b[tAb] copptby[WB] xAxin1[tAb] is detectable
    *** cells: COS<xAxin1><xGsk3b> in BMS
    *** unaffected by: xAxin1(298-832) [substitution]
    *** inhibited by: xAxin1(508-832) [substitution]
    *** inhibited by: xAxin1(713-832) [substitution]
    *** inhibited by: xAxin1(1-229) [substitution]
    *** unaffected by: xAxin1(1-713) [substitution]
    *** unaffected by: xAxin1(298-713) [substitution]
    *** unaffected by: xAxin1(298-506) [substitution]
    *** comment: numbering refers to rat Axin1
    *** source: 9482734-Fig-3b
  *** xGsk3b[tAb] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293T<xAxin1><xGsk3b> in BMS
    *** inhibited by: xAxin1(del(353-437))"delGsk3-binding" [substitution]
    *** source: 11408485-Fig-2
  *** xAxin1[tAb] copptby[WB] xGsk3b[tAb] is detectable
    *** cells: HEK293T<xAxin1><xGsk3b> in BMS
    *** inhibited by: xAxin1(del(353-437))"delGsk3-binding" [substitution]
    *** source: 11408485-Fig-2
  *** Gsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293<xAxin1> in BMS
    *** unaffected by: xAxin1(284-500) [substitution]
    *** unaffected by: xAxin1(284-712) [substitution]
    *** unaffected by: xAxin1(475-676) [substitution]
    *** inhibited by: xAxin1(712-827) [substitution]
    *** source: 11487578-Fig-6a
  *** Gsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: SW480 in BMS
    *** inhibited by: xAxin1(L396P) [substitution]
    *** source: 11487578-Fig-3a

Gsk3b required fearures

  *** xAxin1[tAb] copptby[WB] xGsk3b[tAb] is detectable
    *** cells: COS<xAxin1><xGsk3b> in BMS
    *** inhibited by: xGsk3b(K85M) [substitution]
    *** inhibited by: xGsk3b(Y216F) [substitution]
    *** source: 9482734-Fig-2d
  *** xGsk3b[tAb] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293T<xAxin1><xGsk3b> in BMS
    *** unaffected by: xGsk3b(Y216F) [substitution]
    *** inhibited by: xGsk3b(K83M) [substitution]
    *** source: 11408485-Fig-2
  *** xAxin1[tAb] copptby[WB] xGsk3b[tAb] is detectable
    *** cells: HEK293T<xAxin1><xGsk3b> in BMS
    *** unaffected by: xGsk3b(Y216F) [substitution]
    *** inhibited by: xGsk3b(K83M) [substitution]
    *** source: 11408485-Fig-2

does not require Csnk1e--

  *** Gsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: COS<xAxin1> in BMS
    *** unaffected by: xCsnk1e [addition]
    *** unaffected by: xCsnk1e(mnr)"KD" [addition]
    *** source: 11425858-Fig-6

does not require Dvl1--

  *** Gsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: COS<xAxin1> in BMS
    *** unaffected by: xDvl1 [addition]
    *** source: 11425858-Fig-6

does not require Btrc--

  *** xAxin1[tAb] copptby[WB] xGsk3b[tAb] is detectable
    *** cells: HEK293T<xBtrc><xAxin1> in BMS
    *** does not req: xBtrc [omission]
    *** source: 10228155-Fig-3a

does not require Fbxw2--

  *** xAxin1[tAb] copptby[WB] xGsk3b[tAb] is detectable
    *** cells: HEK293T<xFbxw2><xAxin1> in BMS
    *** does not req: xFbxw2 [omission]
    *** source: 10228155-Fig-3a

No extras

  *** Gsk3b[Ab] copptby[WB] Axin1[Ab] is detectable
    *** cells: Lcells in BMS
    *** source: 11425858-Fig-2
  *** Gsk3b[Ab] copptby[WB] Axin1[Ab] is detectable
    *** cells: Lcells in BMS
    *** source: 11955436-Fig-5b
  *** Gsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293T<xAxin1> in BMS
    *** source: 11955436-Fig-5a
  *** Gsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: COS<xAxin1> in BMS
    *** source: 9482734-Fig-2c
  *** xGsk3b[Ab] copptby[WB] xAxin1[tAb] is detectable
    *** cells: HEK293<xAxin1><xMyc> in BMS
    *** source: 19131971-Fig-1a
  *** xAxin1[tAb] copptby[WB] xGsk3b[tAb] is detectable
    *** cells: COS<xAxin1><xGsk3b> in BMS
    *** source: 9482734-Fig-2b
  *** xAxin1[tAb] copptby[WB] xGsk3b[tAb] is detectable
    *** cells: COS<xAxin1><xGsk3b> in BMS
    *** source: 9482734-Fig-8a
  *** xAxin1[tAb] snaggedby[WB] rGsk3b[GST] is detectable
    *** cells: COS<xAxin1> in BMS
    *** comment: ppt also contains Ctnnb1 [Ab]
    *** source: 9482734-Fig-8b



=======================================================
Coprecipitation with other members of the Axin complex
=======================================================

Apc--  requires Axin1

  *** Gsk3b[Ab] copptby[WB] xApc[tAb] is detectable
    *** cells: COS<xApc><xAxin1> in BMS
    *** reqs: xAxin1 [omission]
    *** source: 11425858-Fig-7
  *** Gsk3b[Ab] copptby[WB] xApc[tAb] is detectable
    *** cells: COS<xApc><xAxin1> in BMS
    *** unaffected by: xCsnk1e [addition]
    *** unaffected by: xCsnk1e(mnr)"KD" [addition]
    *** source: 11425858-Fig-7
  *** Gsk3b[Ab] copptby[WB] xApc[tAb] is detectable
    *** cells: COS<xApc><xAxin1><xCsnk1e> in BMS
    *** unaffected by: xCsnk1e(mnr)"KD" [substitution]
    *** source: 11425858-Fig-7
  *** Gsk3b[Ab] copptby[WB] xApc[tAb] is undetectable
    *** cells: COS<xApc> in BMS
    *** unaffected by: xCsnk1e [addition]
    *** unaffected by: xCsnk1e(mnr)"KD" [addition]
    *** source: 11425858-Fig-7
  *** Gsk3b[Ab] copptby[WB] xApc[tAb] is undetectable
    *** cells: COS<xApc><xCsnk1e> in BMS
    *** unaffected by: xDvl1 [addition]
    *** source: 11425858-Fig-7

Ctnnb1--

  *** xGsk3b[Ab] copptby[WB] Ctnnb1[Ab] is detectable
    *** cells: SW480<xGsk3b> in BMS
    *** unaffected by: xGsk3b(K85M/K86I)"KD" [substitution]
    *** source: 8638126-Fig-1

Csnk1e--  requires Axin1

  *** Gsk3b[Ab] copptby[WB] xCsnk1e[tAb] is detectable
    *** cells: COS<xCsnk1e><xAxin1> in BMS
    *** reqs: xAxin1 [omission]
    *** source: 11425858-Fig-6
  *** Gsk3b[Ab] copptby[WB] xCsnk1e[tAb] is detectable
    *** cells: COS<xCsnk1e><xAxin1> in BMS
    *** unaffected by: xCsnk1e(mnr)"KD" [substitution]
    *** source: 11425858-Fig-6
  *** Gsk3b[Ab] copptby[WB] xCsnk1e[tAb] is detectable
    *** cells: COS<xCsnk1e><xAxin1> in BMS
    *** unaffected by: xDvl1 [addition]
    *** source: 11425858-Fig-6

Dvl1--  requires Axin1

  *** Gsk3b[Ab] copptby[WB] xDvl1[tAb] is detectable
    *** cells: COS<xDvl1><xAxin1> in BMS
    *** reqs: xAxin1 [omission]
    *** source: 11425858-Fig-6
  *** Gsk3b[Ab] copptby[WB] xDvl1[tAb] is detectable
    *** cells: COS<xDvl1><xAxin1> in BMS
    *** unaffected by: xCsnk1e [addition]
    *** unaffected by: xCsnk1e(mnr)"KD" [addition]
    *** source: 11425858-Fig-6


  



























