==================
Direct acetyl
==================

  *** rSmad2[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(mnr)"HAT-domain"
    *** cells: none
    *** source: 17478422-Fig-1b
  *** rSmad2[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(mnr)"HAT-domain"
    *** cells: none
    *** unaffected by: rSmad2(K19R/K20R) [substitution]
    *** source: 17478422-Fig-3d

======================
acetyl itpo Ep300
======================

acetyl(K19)

  *** xSmad2[tAb]IP acetyl(K19)[AcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** bkg inhibited by: xSmad2(K19R) [substitution]
    *** source: 17074756-Fig-3a

acetyl(K19/K20)

  *** xSmad2[tAb]IP acetyl[KAcAb] is detectable
    *** cells: HEK293T<xEp300> in BMS
    *** inhibited by: xSmad2(K19R/K20R) [substitution]
    *** unaffected by: xSmad2(K13R) [substitution]
    *** unaffected by: xSmad2(K39R) [substitution]
    *** unaffected by: xSmad2(K43R/K46R) [substitution]
    *** unaffected by: xSmad2(K50R/K51R) [substitution]
    *** unaffected by: xSmad2(K53R/K54R) [substitution]
    *** unaffected by: xSmad2(K63R) [substitution]
    *** unaffected by: xSmad2(K121R) [substitution]
    *** source: 17074756-Fig-1c
  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T<xSmad2> in BMS
    *** inhibited by: xSmad2(K19R) [substitution]
    *** unaffected by: xSmad2(K20R) [substitution]
    *** source: 17074756-Fig-2b
  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T<xSmad2> in BMS
    *** inhibited by: xSmad2(K19R) [substitution]
    *** source: 17074756-Fig-2c
  *** xSmad2(del(79-108))[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T<xSmad2(del(79-108))> in BMS
    *** inhibited by: xSmad2(del(79-108)/K19R) [substitution]
    *** source: 17074756-Fig-2c
  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T<xSmad2> in BMS
    *** inhibited by: xSmad2(K19R/K20R) [substitution]
    *** inhibited by: xSmad2(K19R/K20R/K39R) [substitution]
    *** inhibited by: xSmad2(K13R/K19R/K20R) [substitution]
    *** unaffected by: xSmad2(K13R) [substitution]
    *** unaffected by: xSmad2(K39R/K43R/K47R) [substitution]
    *** unaffected by: xSmad2(K50R/K51R/K53R/K54R) [substitution]
    *** source: 17478422-Fig-3c

acetyl(K19/K20/K39)

  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** inhibited by: xSmad2(K19R/K20R/K39R) [substitution]
    *** source: 17478422-Fig-4c

================
effect of TgfbR1  ???
================

  *** xSmad2[KAcAb] acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** unaffected by: xTgfbR1(mnr)"CA" [addition]
    *** source: 17478422-Fig-4a
  *** xSmad2[KAcAb] acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** enhanced by: xTgfbR1(mnr)"CA" [addition]
    *** source: 17478422-Fig-4c
  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** enhanced by: xTgfbR1(mnr)"CA" [addition]
    *** source: 17074756-Fig-1b
  *** xSmad2(del(79-108))[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** enhanced by: xTgfbR1(mnr)"CA" [addition]
    *** source: 17074756-Fig-1b


==============
Smad2 features
==============

  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** inhibited by: xSmad2(mnr)"del-GAG-domain" [substitution]
    *** unaffected by: xSmad2(mnr)"del-TID-domain" [substitution]
    *** source: 17478422-Fig-2c
  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** inhibited by: xSmad2(del(79-108)) [substitution]
    *** source: 17074756-Fig-1b
  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** unaffected by: xSmad2(mnr)"Cterm-3S-3A" [substitution]
    *** source: 17478422-Fig-4a

=========
Chemicals
=========

  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEP3B in BMS
    *** enhanced by: TrichostatinA [addition]
    *** source: 17478422-Fig-1d

=========
no extras
=========

  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** source: 17074756-Fig-1a
  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** source: 17478422-Fig-1a
  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** source: 17478422-Fig-2b
  *** xSmad2[tAb]IP acetyl[KAcAb] is increased itpo xEp300
    *** cells: HEK293T in BMS
    *** source: 17478422-Fig-2d


