===============
Kinase Activity
===============

  *** xAkt1[tAb]IP IVKA(HistH2B)[32P-ATP] is increased irt Hgf (times)
    *** cells: MDCK<xAkt1> in BMLS
    *** times: 0 5+ 15+ 30+ 60++ 120++ 180+ 240+ min
    *** source: 9668053-Fig-1b
  *** Akt1[Ab]IP IVKA(HistH2B)[32P-ATP] is increased irt Hgf (10 min)
    *** cells: NIH3T3 in BMLS
    *** bkg inhibited by: LY294002 [pretreatment]
    *** unaffected by: PD98059 [pretreatment]
    *** source: 1134526-Fig-4d
  *** xAkt1[tAb]IP IVKA(HistH2B)[32P-ATP] is increased irt Hgf (10 min)
    *** cells: SKLMS1 in BMLS
    *** inhibited by: Wortmannin [addition]
    *** source: 1134526-Fig-1c
  *** xAkt1[tAb]IP IVKA(HistH2B)[32P-ATP] is increased irt Hgf (10 min)
    *** cells: SKLMS1 in BMLS
    *** source: 1134526-Fig-1b
  *** xAkt1[tAb]IP IVKA(HistH2B)[32P-ATP] is increased irt Hgf (times)
    *** cells: SKLMS1 in BMLS
    *** times: 0 2+ 5+ 10+ 30+ 60+ 120+ min
    *** source: 1134526-Fig-1b
  *** Akt1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt Hgf (times)
    *** cells: rbCornealEpithelialCells in BMLS
    *** times: 0 2+ 5++ 15++ 30+++ min
    *** source: 15452053-Fig-3a

=======================
Phosphorylation on T308
=======================

  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Hgf (times)
    *** cells: mAstrocytes in BMLS
    *** times: 0 5++ 15++ 40+ 90+ min
    *** does not req: Mras [KO]
    *** source: 16980617-Fig-7a
  *** Akt1[phosAb] phos(T308)[phosAb] is increased irt Hgf (times)
    *** cells: MIMCD3 in BMLS
    *** times: 0 2+ 5+ 10+ min
    *** unaffected by: U0126 [pretreatment]
    *** source: 11445578-Fig-4c

=======================
Phosphorylation on S473
=======================

  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Hgf (10 min)
    *** cells: HELA in BMS
    *** inhibited by: xPik3rs(mnr)"DN" [addition]
    *** source: 19525225-Fig-4b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Hgf (30 min)
    *** cells: HCK in BMS
    *** inhibited by: Wortmannin [pretreatment] no-bkg-control
    *** source: 10516287-Fig-6b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Hgf (30 min)
    *** cells: HCK in BMS
    *** inhibited by: Wortmannin [pretreatment] no-bkg-control
    *** unaffected by: PD98059 [pretreatment] no-bkg-control
    *** inhibited by: Genistein [pretreatment] no-bkg-control
    *** unaffected by: Ro318220 [pretreatment] no-bkg-control
    *** source: 10516287-Fig-6a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Hgf (times)
    *** cells: HCK in BMS
    *** times: 0 5+ 10+ 30+ 60+ 90 min
    *** source: 10516287-Fig-4
  *** Akt1[phosAb] phos(S473)[phosAb] is increased itpo xHgf
    *** cells: HCK in BMS
    *** source: 10516287-Fig-5
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Hgf (48 hr)
    *** cells: MDCK in BMS
    *** inhibited by: xGab1 [addition]
    *** inhibited by: xGab1(116-695)"dPH" [addition]
    *** inhibited by: xGab1(Y627F)"dSP" [addition]
    *** partially inhibited by: xGab1(Y447F/Y472F/Y589F)"dPi3k" [addition]
    *** source: 11438654-Fig-4a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Hgf (times)
    *** cells: MDCK in BMS
    *** times: 0 10++ 20++ 120++ 480++ 1440+ min
    *** comment: inhibited by xGab1 [addition] at 120-1440 min
    *** source: 11438654-Fig-4b
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Hgf (5 min)
    *** cells: mTECs in BMLS
    *** unaffected by: xMuc20 [addition]
    *** source: 15314156-Fig-4a
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Hgf (15 min)
    *** cells: rbCornealEpithelialCells in BMLS
    *** inhibited by: LY294002 [pretreatment]
    *** unaffected by: PD98059 [pretreatment]
    *** source: 15452053-Fig-3d
  *** Akt1[phosAb] phos(S473)[phosAb] is increased irt Hgf (times)
    *** cells: rbCornealEpithelialCells in BMLS
    *** times: 0 5+ 15+ 30+ 60+ min
    *** source: 15452053-Fig-3b

==================================
Phosphorylation on unreported site
==================================

  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Hgf (10 min)    
    *** cells: MIMCD3<xPxn> in BMLS
    *** unaffected by: xPxn(S83A) [substitution]
    *** unaffected by: xPxn(Y118A/F120V/P121A) [substitution]
    *** source: 15494312-Fig-4c
  *** Akt1[phosAb] phos(sitenr)[phosAb] is increased irt Hgf (times)
    *** cells: CHANG in BMLS
    *** times: 0 10+ 30++ 60+ 120+ 240 360 480 min
    *** source: 19071214-Fig-1b
