=================
Required Proteins
=================

Ciap1-- and/or Ciap2--

  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: KMS28BM in BMS
    *** times: 0 5 10+ 30+ 60+ min
    *** partially reqs: Ciap1 and/or Ciap2 [null-mut]
    *** source: 18635759-Fig-S8
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 10+ 30+ min
    *** inhibited by: SmacMimetic [addition]
    *** source: 18635759-Fig-6a

Mekk1--  ????

  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0+ 5++ 10++ 30+ 60+ min
    *** bkg inhibited by: kMekk1(1-1188)"del-kinase-domain" [addition]
    *** source: 17143273-Fig-3

Nemo--

  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: 70Z3 in BMS
    *** times: 0 5+ 10+ 30++ 60++ min
    *** reqs: Nemo [KO]
    *** source: 18635759-Fig-2b

Traf2--

  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS
    *** times: 0 2 5+ 10++ 30++ min
    *** reqs: Traf2 [RNAi]
    *** source: 18635759-Fig-1b
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** reqs: Traf2 [KO]
    *** source: 18635759-Fig-1a
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** reqs: Traf2 [KO]
    *** source: 18635759-Fig-S1b
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt Cd40L (10 min)
    *** cells: EJ in BMS
    *** reqs: Traf2 [RNAi]
    *** source: 16260598-Fig-3d
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt Cd40L (20 min)
    *** cells: mEFs<Traf2~null><xTraf2> in BMS
    *** reqs: xTraf2 [omission]
    *** source: 16260598-Fig-5c
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt Cd40L (times)
    *** cells: mEFs in BMS
    *** times: 0 20+ 30+ min
    *** reqs: Traf2 [KO]
    *** source: 16260598-Fig-4b
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt Cd40L (times)
    *** cells: HELA<xCd40> in BMS
    *** times: 0 20++ 30+ min
    *** inhibited by: xCd40(T254A)"mT2/T3" [substitution]
    *** inhibited by: xCd40(Q234A/E235A/Q263A/T254A)"mT2/T3/T6" [substitution]
    *** source: 16260598-Fig-2b

not Traf3--

  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS
    *** times: 0 2 5+ 10++ 30++ min
    *** does not req: Traf3 [RNAi]
    *** comment: signal is enhanced at 5 min in Traf3-KO cells
    *** source: 18635759-Fig-1b
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** does not req: Traf3 [KO]
    *** source: 18635759-Fig-1a
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** does not req: Traf3 [KO]
    *** source: 18635759-Fig-S1b

Traf6--

  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS
    *** times: 0 2 5+ 10++ 30++ min
    *** partially reqs: Traf6 [RNAi]
    *** source: 18635759-Fig-1b
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** partially reqs: Traf6 [KO]
    *** source: 18635759-Fig-1a
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** partially reqs: Traf6 [KO]
    *** source: 18635759-Fig-S1b
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt Cd40L (20 min)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** bkg reqs: xTraf6 [omission]
    *** source: 16260598-Fig-7b
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt Cd40L (times)
    *** cells: mEFs in BMS
    *** times: 0 20+ 30 min
    *** reqs: Traf6 [KO]
    *** source: 16260598-Fig-6d
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt Cd40L (tnr)
    *** cells: HELA<xCd40(Q234A/E235A)"mT6"> in BMS
    *** reqs: Traf6 [RNAi]
    *** source: 16260598-Fig-8f

======================
Cd40 required features
======================

  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt Cd40L (times)
    *** cells: HELA<xCd40> in BMS
    *** times: 0 20++ 30+ min
    *** unaffected by: xCd40(Q234A/E235A)"mT6" [substitution]
    *** inhibited by: xCd40(Q234A/E235A/Q263A/T254A)"mT2/T3/T6" [substitution]
    *** unaffected by: xCd40(Q234A/E235A/Q263A)"mT3/T6" [substitution]
    *** source: 16260598-Fig-2b
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt Cd40L (15 min)
    *** cells: HEK293<xCd40> in BMS
    *** inhibited by: xCd40(T254A) [substitution]
    *** source: 10433725-Fig-6b
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (5 min)
    *** cells: mSpCs<Cd40~null><xCd40> in BMS
    *** unaffected by: xCd40(del(230-247))"dTR6" [substitution]
    *** inhibited by: xCd40(T255A)"dTR23" [substitution]
    *** inhibited by: xCd40(del(230-247)/T255A)"dTRs" [substitution]
    *** source: 12354380-Fig-7b
  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (5 min)
    *** cells: mSpCs<Cd40~pos> in BMS
    *** source: 12354380-Fig-7b

===================================
Requirement for Proteasome Activity
===================================

  *** P38s[phosAb] phos(TGY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 10+ 30+ min
    *** inhibited by: MG132 [addition]
    *** source: 18635759-Fig-6b

