=====================
Relocation to nucleus
=====================

Rsk2--

  *** Nfkb1{p50}[Ab] infraction(nuclear)[WB] is increased irt Tnf (times)
    *** cells: HELA in BMLS "nuclear fraction"
    *** times: 0 10+ 30+ min
    *** reqs: Rsk2 [RNAi]
    *** source: 20385620-Fig-4b
  *** Nfkb1{p50}[Ab] infraction(nuclear)[WB] is increased irt Tnf (times)
    *** cells: mEFs in BMLS "nuclear fraction"
    *** times: 0 10+ 30+ min
    *** reqs: Rsk2 [RNAi]
    *** source: 20385620-Fig-4e

not Tpl2--

  *** Nfkb1{p50}[Ab] infraction(nuclear)[WB] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30++ min
    *** does not req: Tpl2 [KO]
    *** source: 15833743-Fig-5b


============
DNA binding
============

  *** Nfkb1{p50}[Ab] oligo-binding[EMSA] is increased irt Tnf (15 min)
    *** cells: HEK293 in BMS "nuclear-extracts"
    *** source: 10581243-Fig-5a
  *** Nfkb1{p50}[Ab] oligo-binding[EMSA] is increased irt Tnf (times)
    *** cells: HELA in BMLS "nuclear fraction"
    *** times: 0 10+ 30+ min
    *** partially reqs: Rsk2 [RNAi]
    *** source: 20385620-Fig-4b
  *** Nfkb1{p50}[Ab] snaggedby[WB] oligo[biotin] is increased irt Tnf (times)
    *** cells: A549 in BMS
    *** times: 0 15+ 30+ 60+ min
    *** source: 15677444-Fig-4a
  *** Nfkb1[Ab] oligo-binding[EMSA] is increased irt Tnf (15 min)
    *** cells: MCF7 in BMS
    *** comment: binding is inhibited by addition of rPias1 to lysates
    *** source: 15657437-Fig-7a
  ---------------- contradiction ----------------------------------------
  *** Nfkb1{p50}[Ab] oligo-binding[EMSA] is unchanged irt Tnf (30 min)
    *** cells: mEFs in BMS
    *** source: 9390694-Fig-6b


====================
Binding to Importins
====================

Kpna3--

  *** Nfkb1{p50}[Ab] snaggedby[WB] rKpna3[GST] is increased irt Tnf (30 min)
    *** cells: A549 in BMS
    *** inhibited by: rKpna3(mnr)"ARM3" [substitution]
    *** unaffected by: rKpna3(mnr)"ARM7+8" [substitution]
    *** source: 15677444-Fig-7c
  *** Nfkb1{p50}[Ab] snaggedby[WB] rKpna3[GST] is increased irt Tnf (30 min)
    *** cells: A549 in BMS
    *** source: 15677444-Fig-1a
  *** Nfkb1{p50}[Ab] snaggedby[WB] rKpna3[GST] is increased irt Tnf (30 min)
    *** cells: A549 in BMS
    *** source: 15677444-Fig-1b
  *** Nfkb1{p50}[Ab] snaggedby[WB] rKpna3[GST] is increased irt Tnf (times)
    *** cells: A549 in BMS
    *** times: 0 1 2 4 8+ 15++ 30+++ 60+++ 120+++ min
    *** source: 15677444-Fig-3b

Kpna4--

  *** Nfkb1{p50}[Ab] snaggedby[WB] rKpna4[GST] is increased irt Tnf (30 min)
    *** cells: A549 in BMS
    *** source: 15677444-Fig-1b

















