==============================================
Nemo binds with K63 poly chains
requires Nemo sites: L329 D311 I307 Y306 F312
==============================================

  *** rNemo[Ab] boundby[WB] rUbiq{mono}[Sepharose] is undetectable
    *** cells: none
    *** source: 16603398-Fig-6a
  *** rNemo[Ab] boundby[WB] rUbiq{K48poly}[Sepharose] is detectable
    *** cells: none
    *** comment: rNemo "prefers" binding to K63-linked-ubiquitin
    *** source: 16603398-Fig-6a
  *** rNemo[Ab] boundby[WB] rUbiq{K48poly}[Sepharose] is detectable
    *** cells: none
    *** inhibited by: rNemo(I307A) [substitution]
    *** inhibited by: rNemo(Y308S) [substitution]
    *** inhibited by: rNemo(F312A) [substitution]
    *** source: 16603398-Fig-7c
  *** rNemo[Ab] boundby[WB] rUbiq{K63poly}[Sepharose] is detectable
    *** cells: none
    *** source: 16603398-Fig-6a
  *** rNemo[Ab] boundby[WB] rUbiq{K63poly}[Sepharose] is detectable
    *** cells: none
    *** unaffected by: rNemo(I307A) [substitution]
    *** inhibited by: rNemo(Y308S) [substitution]
    *** inhibited by: rNemo(F312A) [substitution]
    *** source: 16603398-Fig-7c

=======================
Required Nemo features
=======================

  *** rUbiq{K63poly}[Ab] boundby[WB] rNemo(242-350)[GST] is detectable
    *** cells: none
    *** source: 16547522-Fig-2c
  *** rUbiq{K63poly}[Ab] boundby[WB] rNemo[GST] is detectable
    *** cells: none
    *** inhibited by: rNemo(L329P) [substitution]
    *** inhibited by: rNemo(D311N) [substitution]
    *** source: 16547522-Fig-2d
  *** rUbiq{K63poly}[Ab] boundby[WB] rNemo[GST] is detectable
    *** cells: none
    *** inhibited by: rUbiq{K48poly} [substitution]
    *** source: 16547522-Fig-2a
  *** rUbiq{K63poly}[Ab] boundby[WB] rNemo[GST] is detectable
    *** cells: none
    *** unaffected by: rNemo(108-419) [substitution]
    *** inhibited by: rNemo(108-312) [substitution]
    *** source: 16547522-Fig-2b
  *** rNemo[35S] boundby[WB] rUbiq{dimer}[GST] is detectable
    *** cells: none
    *** inhibited by: rNemo(D311N) [substitution]
    *** source: 16547522-Fig-1e
  *** rNemo[35S] boundby[WB] rUbiq{dimer}[GST] is detectable
    *** cells: none
    *** inhibited by: rNemo(L329P) [substitution]
    *** source: 16547522-Fig-1d
  *** rNemo[35S] boundby[WB] rUbiq{dimer}[GST] is detectable
    *** cells: none
    *** inhibited by: rUbiq{mono} [substitution]
    *** source: 16547522-Fig-1a
  *** rNemo[35S] boundby[WB] rUbiq{dimer}[GST] is detectable
    *** cells: none
    *** unaffected by: rNemo(108-419) [substitution]
    *** unaffected by: rNemo(1-395) [substitution]
    *** inhibited by: rNemo(108-312) [substitution]
    *** source: 16547522-Fig-1b

  *** xNemo[tAb]IP ubiq[tUbAb] is increased itpo xUbiq
    *** cells: HEK293T in BMS
    *** unaffected by: xNemo(del(393-419)) [substitution]
    *** unaffected by: xNemo(C417R) [substitution]
    *** source: 12867425-Fig-2a

===========
Other Clues
===========

  *** xNemo[tAb]IP ubiq[tUbAb] is increased itpo xUbiq
    *** cells: HEK293T in BMS
    *** inhibited by: xUbiq(G76A) [substitution]
    *** source: 12867425-Fig-1c
  *** xNemo[tAb]IP ubiq[tUbAb] is increased itpo xUbiq
    *** cells: HEK293T in BMS
    *** unaffected by: MG132 [addition]
    *** source: 12867425-Fig-1d

================
Coprecipitations
================

Ciap1--

  *** Nemo[Ab] copptby[WB] Ciap1[Ab] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: Tnf (10 min) [stim]
    *** source: 12867425-Fig-4d

Irf1--

  *** Nemo[Ab] copptby[WB] Irf7[Ab] is undetectable
    *** cells: mBMfDCs in BMS
    *** source: 16612387-Fig-3d

Ppp2cb--

  *** Nemo[Ab] copptby[WB] Ppp2cb[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 14585847-Fig-1d
  *** Nemo[Ab] copptby[WB] xPpp2cb[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 14585847-Fig-1b

Ripk1--

  *** Nemo[Ab] copptby[WB] xRipk1[tAb] is detectable
    *** cells: HEK293 in BMS
    *** partially inhibited by: xRipk1(K377R) [substitution]
    *** unaffected by: xRipk1(K396R) [substitution]
    *** unaffected by: xRipk1(K530R) [substitution]
    *** source: 16543241-Fig-5b

Tank--

  *** xNemo[Ab] copptby[WB] xTank[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 12133833-Fig-2b

Traf2--

  *** xNemo[tAb] copptby[WB] xTraf2[tAb] is detectable
    *** cells: HEK293T in BMS
    *** enhanced by: xCiap1 [addition]
    *** unaffected by: xCiap1(122-618) [addition]
    *** source: 12867425-Fig-4f

Traf3ip2--

  *** xNemo[Ab] copptby[WB] xTraf3ip2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 12133833-Fig-4d

Zfand5--

  *** Nemo[Ab] copptby[WB] xZfand5[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 14754897-Fig-2b
