

Ubc13

  *** Map4k5[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tnf (15 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: xUbc13(C87A) + xUbiq(K63R) [addition]
    *** comment: inhibited by cotransfection of Ubc13-AS-DNA and Ubiq(K63R)
    *** source: 12591926-Fig-2c
  *** xMap4k5[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tnf (15 min)
    *** cells: HEK293 in BMS
    *** inhibited by: xUbc13(C87A)"E2-inactive" [addition]
    *** source: 12591926-Fig-3
  *** xMap4k5[tAb] oligomerization[2TagPpt] is increased irt Tnf (15 min)
    *** cells: HEK293 in BMS
    *** inhibited by: xUbc13(C87A)"E2-inactive" [addition]
    *** source: 12591926-Fig-4b

Traf2

  *** xMap4k5[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Tnf (15 min)
    *** cells: HEK293T in BMS
    *** inhibited by: xTraf2(87-501)"DN" [addition]
    *** source: 9405407-Fig-3f


something bound to Map4k5 is ubiqed irt Tnf
something ubiqed is bound to Map4k5 irt Tnf

  *** xMap4k5[tAb]IP ubiq[UbAb] is increased irt Tnf (15 min)
    *** cells: HELA in BMS
    *** inhibited by: xTraf2(C34A)"E3-inactive" [addition]
    *** source: 12591926-Fig-5c
  *** xMap4k5[tAb]IP ubiq[UbAb] is increased irt Tnf (15 min)
    *** cells: HELA in BMS
    *** inhibited by: xUbc13(C87A)"E2-inactive" [addition]
    *** inhibited by: xUbiq(K63R) [addition]
    *** inhibited by: xTraf2(87-501)"DN" [addition]
    *** source: 12591926-Fig-5b

in TnfR1 complex?

  *** Map4k5[Ab] copptby[WB] xTraf2[GST] is increased irt Tnf (15 min)
    *** cells: HEK293 in BMS
    *** inhibited by: xUbc13(C87A)"E2-inactive" [addition]
    *** source: 12591926-Fig-4a

  *** Map4k5[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tnf (15 min)
    *** cells: HELA in BMS
    *** source: 9405407-Fig-3e
  *** Map4k5[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tnf (times)
    *** cells: HEK293T in BMS
    *** times: 0 10+ 20+ 30+ min
    *** source: 9405407-Fig-3a
