====================
Requirement for IL6R
====================

  *** IL6[125I] receptor-binding[RIA] is undetectable
    *** cells: BAFB03<IL6R~null><xGp130> in BMS
    *** comment: IL6 is from human
    *** source: 2261637-Fig-5a
  *** IL6R[35S] boundby[Xlink] IL6[Ab] is detectable
    *** cells: JURKAT<Pten~null><xIL6R> in BMS
    *** reqs: xIL6R [omission]
    *** source: 2788034-Fig-2b
  *** IL6R[35S] boundby[Xlink] IL6[Ab] is detectable
    *** cells: U266 in BMS
    *** source: 2788034-Fig-2b

=====================
Requirement for Gp130
=====================

coppt with IL6R--

  *** Gp130[35S] copptby[WB] IL6R[Ab] is increased irt IL6 (30 min)
    *** cells: U266 in BMS
    *** comment: inhibited at 0oC
    *** source: 2788034-Fig-3b
  *** Gp130[35S] copptby[WB] IL6R[Ab] is increased irt IL6 (30 min)
    *** cells: U266 in BMS
    *** source: 2788034-Fig-3a
  *** Gp130[125I] copptby[WB] IL6R[Ab] is increased irt IL6 (30 min)
    *** cells: HEPG2 in BMS
    *** source: 2788034-Fig-3e
  *** Gp130[125I] copptby[WB] IL6R[Ab] is increased irt IL6 (30 min)
    *** cells: M12<xIL6R> in BMS
    *** source: 2788034-Fig-4a
  *** Gp130[125I] copptby[WB] IL6R[Ab] is increased irt IL6 (30 min)
    *** cells: U266 in BMS
    *** comment: coppt remains after treatment with nueuraminidase, N-, and O-glycanase
    *** source: 2788034-Fig-3d
  *** Gp130[125I] copptby[WB] IL6R[Ab] is increased irt IL6 (30 min)
    *** cells: U266 in BMS
    *** source: 2261637-Fig-1a
  *** Gp130[125I] copptby[WB] IL6R[Ab] is increased irt IL6 (times)
    *** cells: U266 in BMS
    *** times: 0 5+ 10+ 20+ 40++ min
    *** source: 2788034-Fig-3c

  *** IL6[125I] receptor-binding[RIA] is increased itpo xGp130
    *** cells: JURKAT<Pten~null><xIL6R> in BMS
    *** source: 2261637-Fig-5b
  *** GRR-reporter[Luc] is increased irt IL6 (2 hr)
    *** cells: HEPG2 in BMS
    *** inhibited by: anti-Gp130 [pretreatment]
    *** source: 10954742-Fig-5a

coppt with IL6Rsol--

  *** xGp130[35S] copptby[WB] IL6Rsol[Ab] is increased irt IL6 (tnr) 
    *** cells: BAFB03<IL6R~null><xGp130> in BMS
    *** source: 2261637-Fig-6
  *** Gp130[125I] copptby[WB] IL6Rsol[Ab] is increased irt IL6 (30 min)
    *** cells: M1 in BMS
    *** source: 2788034-Fig-6a
  *** xGp130[35S] copptby[WB] IL6Rsol[Ab] is detectable
    *** cells: BAFB03<xGp130> in BMS "treated with IL6"
    *** unaffected by: xGp130(1-783)"IC141" [substitution]
    *** unaffected by: xGp130(1-735)"IC93" [substitution]
    *** unaffected by: xGp130(1-707)"IC65" [substitution]
    *** unaffected by: xGp130(1-652)"IC10" [substitution]
    *** source: 1662392-Fig-2

=================================
Gp130 Phosphorylation on Tyrosine
=================================

reqs Jak1--

  *** Gp130[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: U4A<Jak1~null> in BMS
    *** enhanced by: xJak1 [addition]
    *** source: 7537214-Fig-4
  *** Gp130[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: U4A<Jak1~null><xJak1> in BMS
    *** reqs: xJak1 [omission]
    *** source: 7537214-Fig-8b

not Jak2--

  *** Gp130[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: GAMMA2A<Jak2~null><xJak2> in BMS
    *** does not req: xJak2 [omission]
    *** unaffected by: xJak2(mnr)"KD" [substitution]
    *** source: 7537214-Fig-8a
  *** Gp130[Ab]IP Yphos[pYAb] is unchanged irt (IL6 + IL6Rsol) (15 min)
    *** cells: U4A<Jak1~null> in BMS
    *** unaffected by: xJak2 [addition]
    *** source: 7537214-Fig-8b

inhibited by Ptprec--

  *** Gp130[Ab]IP Yphos[pYAb] is increased irt IL6 (10 min)
    *** cells: M1 in BMS
    *** partially inhibited by: xPtprec [addition]
    *** unaffected by: xPtprec(D245A) [addition]
    *** source: 10859312-Fig-6b

No extras

  *** Gp130[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (5 min)
    *** cells: EW1 in BMLS
    *** source: 8272873-Fig-1a
  *** Gp130[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: 2FTGH in BMS
    *** source: 7537214-Fig-4
  *** Gp130[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: 2FTGH in BMS
    *** source: 7537214-Fig-8b
  *** Gp130[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: GAMMA2A<Jak2~null> in BMS
    *** source: 7537214-Fig-4
  *** Gp130[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: U1D<Tyk2~null> in BMS
    *** source: 7537214-Fig-4
  *** xGp130[Ab]IP Yphos[pYAb] is increased irt (IL6Rsol + IL6) (10 min) 
    *** cells: BAFB03<xGp130> in BMLS
    *** source: 1662392-Fig-5
  *** Gp130[Ab]IP Yphos[pYAb] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** source: 8272872-Fig-4a
  *** Gp130[Ab]IP Yphos[pYAb] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** source: 9632795-Fig-1c
  *** Gp130[Ab]IP Yphos[pYAb] is increased irt IL6 (times)
    *** cells: HEPG2 in BMS
    *** times: 0 5++ 10+ 20+ 40 min
    *** source: 9794795-Fig-1c
  *** xGp130[Ab]IP phos[32Pi] is increased irt (IL6 + IL6Rsol) (10 min)
    *** cells: HEP3B in BMS
    *** comment: the increased phos is mostly on Tyrosine [PAA]
    *** source: 8511589-Fig-2a

==================
Gp130 Dimerization
==================

  *** xGp130[Ab]IP oligomerization[NativeWB] is increased irt (IL6 + IL6Rsol) (10 min)
    *** cells: HEP3B in BMS
    *** source: 8511589-Fig-1a
  *** xGp130[Ab]IP oligomerization[NativeWB] is increased irt (IL6 + IL6Rsol) (10 min)
    *** cells: HEP3B in BMS
    *** unaffected by: xGp130(P656S/P658S) [substitution]
    *** source: 8511589-Fig-4b

=================================================
Coprecipitation of something with kinase activity
=================================================

  *** Gp130[Ab]IP IVKA(auto)[32P-ATP] is increased irt (IL6 + IL6Rsol) (5 min)
    *** cells: EW1 in BMLS
    *** source: 8272873-Fig-1a
  *** xGp130[Ab]IP IVKA(auto)[32P-ATP] is increased irt (IL6 + IL6Rsol) (10 min)
    *** cells: HEP3B in BMS "RCA120 binding glycoprotein fraction"
    *** source: 8511589-Fig-3

