================================================================
Phosphorylation occurs at sight of DSBs but Chek1 does not bind
================================================================

  *** Chek1[Ab] locatedin(DSBs)[IHC] is unchanged irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** comment: does not colocalize with H2ax-phos(S140)
    *** source: 16618811-Fig-7a
  *** Chek1[phosAb] phos(S317)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** comment: Chek1 remains in nucleus but does not colocalize with H2ax-phos(S140)
    *** source: 16618811-Fig-7a
  *** xChek1[XFP] locatedin(DSBs)[IHC] is unchanged irt MicroLaser (1 hr)
    *** cells: U2OS<xChek1> in BMS
    *** source: 16618811-Fig-9a
  *** xChek1[XFP] locatedin(subnuclear-bodies)[IHC] is unchanged irt IR (1 hr)
    *** cells: U2OS<xChek1> in BMS
    *** source: 16618811-Fig-9b


=======================
Phosphorylation on S317
=======================

Atm--  ???

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** partially reqs: Atm [RNAi]
    *** source: 16431910-Fig-1a
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** does not req: Atm [RNAi]
    *** source: 16431910-Fig-S2
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 15+ 30+ 45+ min
    *** partially reqs: Atm [RNAi]
    *** source: 16431910-Fig-1c
  *** Chek1[phosAb] phos(S317)[phosAb] is unchanged irt IR (1 hr)
    *** cells: hFibros<Atm~fmut> in BMS
    *** source: 16327781-Fig-1a

Atr--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** reqs: Atr [RNAi]
    *** source: 16431910-Fig-1a
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** reqs: Atr [RNAi]
    *** source: 16431910-Fig-S2
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 15+ 30+ 45+ min
    *** reqs: Atr [RNAi]
    *** source: 16431910-Fig-1b
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Atr [RNAi]
    *** source: 16327781-Fig-1b
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Atr [RNAi]
    *** source: 16327781-Fig-1c
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Atr [RNAi]
    *** source: 16327781-Fig-S1a
  *** Chek1[phosAb] phos(S317)[phosAb] is unchanged irt IR (1 hr)
    *** cells: hFibros<Atr~fmut> in BMS
    *** source: 16327781-Fig-1a

Claspin--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Claspin [RNAi]
    *** source: 16327781-Fig-S1b

Mre11--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** reqs: Mre11 [RNAi]
    *** source: 16431910-Fig-1a
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: hFibros<Mre11~fmut><xMre11> in BMS
    *** reqs: xMre11 [omission]
    *** inhibited by: xMre11(mnr)"CIA" [substitution]
    *** source: 16327781-Fig-4a

Nbs1--  ???

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: hFibros<Nbs1~fmut><xNbs1> in BMS
    *** partially reqs: xNbs1 [omission]
    *** unaffected by: xNbs1(S278A/S343A/S397A) [substitution]
    *** source: 16327781-Fig-2c

not Rnf8--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Rnf8 [RNAi]
    *** source: 18001824-Fig-S7d

not Topbp1--

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Topbp1 [RNAi]
    *** source: 16327781-Fig-S1b

chemicals

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** inhibited by: KU55933 [pretreatment]
    *** source: 16327781-Fig-1b
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (30 min)
    *** cells: U2OS in BMS
    *** bkg inhibited by: Roscovitine [pretreatment]
    *** source: 16327781-Fig-7a

no extras

  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** source: 16327781-Fig-1a
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (30 min)
    *** cells: T24 in BMS
    *** comment: cells were synchronized at G0 by density arrest
    *** comment: response seen only after 20 hr post replating (S phase)
    *** source: 16327781-Fig-6e
  *** Chek1[phosAb] phos(S317)[phosAb] is increased irt IR (times)
    *** cells: U2OS in BMS
    *** times: 0 5 10+ 15++ 30++ 60++ min
    *** source: 16327781-Fig-5b

=======================
Phosphorylation on S345
=======================

Atm--  ???

  *** Chek1[phosAb] phos(S345)[phosAb] is unchanged irt IR (45 min)
    *** cells: GM18366<Atr~fmut> in BMS
    *** source: 16461339-Fig-1c
  *** Chek1[phosAb] phos(S345)[phosAb] is unchanged irt IR (45 min)
    *** cells: NotReported<Atm~fmut> in BMS
    *** source: 16461339-Fig-1b

Mdc1--  ???

  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt IR (times)
    *** cells: NotReported in BMS
    *** times: 0 1+ 2+ hr
    *** partially reqs: Mdc1 [RNAi]
    *** source: 12607005-Fig-5e

chemicals

  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt IR (45 min)
    *** cells: RAJI in BMS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 16461339-Fig-1a
  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt IR (45 min)
    *** cells: RAJI in BMS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 16461339-Fig-S1

no extras

  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0+ 15++ 30++ 45++ min
    *** source: 16431910-Fig-1b
  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt IR (45 min)
    *** cells: GM12072 in BMS
    *** source: 16461339-Fig-1c
  *** Chek1[phosAb] phos(S345)[phosAb] is increased irt IR (45 min)
    *** cells: NotReported in BMS
    *** source: 16461339-Fig-1b

===================================
Phosphorylation on Unreported Site
===================================

  *** Chek1[phosAb] phos(sitenr)[phosAb] is increased irt IR (30 min)
    *** cells: mEFs in BMS
    *** reqs: H2ax [KO]
    *** source: 16427009-Fig-6b
  *** Chek1[phosAb] phos(sitenr)[phosAb] is increased irt IR (30 min)
    *** cells: mEFs in BMS
    *** reqs: Mdc1 [KO]
    *** source: 16427009-Fig-6b

