============================
Mek1 phosphorylation by Pak1
============================

  *** rMek1(265-301)[32P] phos[32P-ATP] is increased by rPak1
    *** cells: none
    *** source: 9677429-Fig-4
  *** rMek1[32P] phos[32P-ATP] is increased by xPak1(165-544)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293 in BMS
    *** partially inhibited by: rMek1(T292A) [substitution]
    *** inhibited by: rMek1(S298A) [substitution]
    *** inhibited by: rMek1(T292A/S298A) [substitution]
    *** source: 11948406-Fig-3a
  *** rMek1[phosAb] phos(S298)[phosAb] is increased by xPak1(165-544)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293 in BMS
    *** source: 11948406-Fig-3b
  *** rMek1[phosAb] phos(T292)[phosAb] is unchanged by xPak1(165-544)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293 in BMS
    *** source: 11948406-Fig-3b

============================================================
Mek1 phosphorylation on S218/S222 in the presence of activated Pak1
requires Mek1 kinase activity
============================================================

  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased itpo xPak1(T423E)"CA"
    *** cells: rEFs in BMLS "in suspension"
    *** inhibited by: xMek1(S298A) [substitution]
    *** inhibited by: xMek1(K97A)"KD" [substitution]
    *** inhibited by: U0126 [addition]
    *** source: 17314031-Fig-1,2a
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased itpo xPak1(T423E)"CA"
    *** cells: REF52 in BMS
    *** enhanced by: PP2 [addition]
    *** source: 12876277-Fig-7b
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased itpo xPak1(T423E)"CA"
    *** cells: REF52 in BMS
    *** inhibited by: xMek1(S298A) [substitution]
    *** source: 12876277-Fig-7b
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased itpo xPak1(165-544)"CA"
    *** cells: HEK293 in BMLS
    *** enhanced by: xRaf1 [addition]
    *** inhibited by: xRaf1(S338A/S339C) [addition]
    *** inhibited by: xRaf1(S338D/S339E) [addition]
    *** source: 11948406-Fig-1b

===============================================================
Mek1 phosphorylation on S298 in the presence of activated Pak1
does not require Mek1 kinase activity
===============================================================

  *** xMek1[tAb]IP phos(S298)[phosAb] is increased itpo xPak1(T423E)"CA"
    *** cells: rEFs in BMLS "in suspension"
    *** inhibited by: xMek1(S298A) [substitution]
    *** unaffected by: xMek1(K97A)"KD" [substitution]
    *** unaffected by: U0126 [addition]
    *** source: 17314031-Fig-1,2a
  *** xMek1[tAb]IP phos(S298)[phosAb] is increased itpo xPak1(T423E)"CA"
    *** cells: COS1 in BMLS "in suspension"
    *** source: 14993270-Fig-5b
  *** xMek1[tAb]IP phos(S298)[phosAb] is increased itpo xPak1(165-544)"CA"
    *** cells: HEK293 in BMLS
    *** unaffected by: xRaf1 [addition]
    *** source: 11948406-Fig-4a
  *** xMek1[tAb]IP phos(T292)[phosAb] is increased itpo xPak1(165-544)"CA"
    *** cells: HEK293 in BMLS
    *** unaffected by: xRaf1 [addition]
    *** source: 11948406-Fig-4b
  *** xMek1[tAb]IP phos(S298)[phosAb] is increased itpo xPak1(T423E)"CA"
    *** cells: REF52 in BMS
    *** unaffected by: Fn1-adherence (20 min) [stim]
    *** source: 12876277-Fig-4b
  *** xMek1[tAb]IP phos(S298)[phosAb] is increased itpo xPak1(T423E)"CA"
    *** cells: REF52 in BMS
    *** unaffected by: PP2 [addition]
    *** source: 12876277-Fig-7b
