requires Raf1 phos(S621)

  *** rYwhaz[GST] boundby[SPR] xRaf1[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9<xRaf1> in BMS
    *** inhibited by: xRaf1(S621A) [substitution]
    *** source: 14688280-Fig-6a
  *** xRaf1[tAb] copptby[WB] xYwhaz[GST] is detectable
    *** cells: COS7 in BMS
    *** comment: inhibited by addition rRaf1-phos(S621)-peptide to lysate
    *** source: 10998357-Fig-4
  *** rYwhaz[GST] boundby[SPR] xRaf1[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9<xRaf1> in BMS
    *** inhibited by: anti-Raf1pS621 [addition]
    *** source: 14688280-Fig-5a
  *** rYwhaz[GST] boundby[SPR] xRaf1[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9<xRaf1> in BMS
    *** inhibited by: sRaf1(mnr/pS621) [addition]
    *** source: 14688280-Fig-5b

requires Raf1 phos(S259)  ???

  *** rYwhaz[GST] boundby[SPR] xRaf1[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9<xRaf1> in BMS
    *** inhibited by: xRaf1(S259A) [substitution]
    *** source: 14688280-Fig-6a
  *** rYwhaz[GST] boundby[SPR] xRaf1[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9<xRaf1> in BMS
    *** unaffected by: anti-Raf1pS259 [addition]
    *** source: 14688280-Fig-5a
  *** rYwhaz[GST] boundby[SPR] xRaf1[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9<xRaf1> in BMS
    *** unaffected by: sRaf1(mnr/pS259) [addition]
    *** source: 14688280-Fig-5b

does not req Raf1 phos(S471) or phos(S481)

  *** xRaf1[tAb] copptby[WB] xYwhaz[tAb] is detectable
    *** cells: COS7 in BMS
    *** unaffected by: xRaf1(S471A) [substitution]
    *** unaffected by: xRaf1(S481A) [substitution]
    *** source: 16093354-Fig-10c

combined mutations (or why do people do these experiments?)

  *** rYwhaz[GST] boundby[SPR] xRaf1[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9<xRaf1> in BMS
    *** inhibited by: xRaf1(S259A/S621A) [substitution]
    *** source: 14688280-Fig-6a
  *** xRaf1(S621A)[tAb] copptby[WB] xYwhaz[tAb] is detectable
    *** cells: COS in BMS
    *** inhibited by: xRaf1(S257L/S621A) [substitution]
    *** inhibited by: xRaf1(S259A/S621A) [substitution]
    *** source: 12077328-Fig-4c
  *** xRaf1[tAb] copptby[WB] xYwhaz[tAb] is detectable
    *** cells: COS in BMS
    *** inhibited by: xRaf1(S257L/S621A) [substitution]
    *** inhibited by: xRaf1(S259A/S621A) [substitution]
    *** source: 12077328-Fig-3c


Hras--  ???

  *** xYwhaz[tAb] copptby[WB] xRaf1[tAb] is detectable
    *** cells: COS in BMS
    *** inhibited by: xHras(G12V)"CA" [addition]
    *** partially inhibited by: xHras(G12V/E37G)"CA" [addition]
    *** source: 12077328-Fig-6a

Cbl--  ???

  *** Raf1[Ab] snaggedby[WB] rYwhaz[GST] is detectable
    *** cells: JURKATT in BMS
    *** enhanced by: xCbl [addition]
    *** source: 9092538-Fig-3b

Cblb--  ???

  *** Raf1[Ab] snaggedby[WB] rYwhaz[GST] is detectable
    *** cells: JURKATT in BMS
    *** enhanced by: xCblb [addition]
    *** source: 9092538-Fig-3b

chemicals

  *** Raf1[Ab] snaggedby[WB] rYwhaz[GST] is detectable
    *** cells: JURKATT in BMS
    *** unaffected by: PMA (15 min) [stim]
    *** source: 9092538-Fig-3b

no extras

  *** rYwhaz[GST] boundby[SPR] xRaf1[Ab]IP is detectable
    *** cells: none
    *** IPfrom: SF9<xRaf1> in BMS
    *** source: 14688280-Fig-7a
  *** xRaf1[His]IP boundby[WB] rYwhaz[GST] is detectable
    *** cells: none
    *** IPfrom: SF9<xRaf1> in BMS
    *** source: 14688280-Fig-7c
