  *** rNemo[tAb] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** reqs: rUbe1 [omission]
    *** reqs: rUbe2d3 [omission]
    *** reqs: rCiap1 [omission]
    *** source: 12867425-Fig-3c
  *** rNemo[tAb] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** source: 12867425-Fig-3e

================================================================
Ubiq features required for Nemo ubiquitination by Ube2d3 + Ciap1
================================================================

  *** rNemo ubiq[tUbAb] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** inhibited by: rUbiq(dig)"no-lysines" [substitution]
    *** partially inhibited by: rUbiq(K11R/K27R/K29R/K33R/K48R/K63R)"K6" [substitution]
    *** inhibited by: rUbiq(K6R/K27R/K29R/K33R/K48R/K63R)"K11" [substitution]
    *** inhibited by: rUbiq(K6R/K11R/K29R/K33R/K48R/K63R)"K27" [substitution]
    *** inhibited by: rUbiq(K6R/K11R/K27R/K33R/K48R/K63R)"K29" [substitution]
    *** inhibited by: rUbiq(K6R/K11R/K27R/K29R/K48R/K63R)"K33" [substitution]
    *** inhibited by: rUbiq(K6R/K11R/K27R/K29R/K33R/K63R)"K48" [substitution]
    *** inhibited by: rUbiq(K6R/K11R/K27R/K29R/K33R/K48R)"K63" [substitution]
    *** source: 12867425-Fig-3i
  *** rNemo[Ab]IP ubiq[tUbAb] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** inhibited by: rUbiq(dig)"no-lysines" [substitution]
    *** partially inhibited by: rUbiq(K11R/K27R/K29R/K33R/K48R/K63R)"K6" [substitution]
    *** source: 12867425-Fig-3k
  *** rNemo[tAb] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** reqs: rUbiq [omission]
    *** partially inhibited by: rUbiq(dig)"no-lysines" [substitution]
    *** source: 12867425-Fig-3g
  *** rNemo[tAb] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** inhibited by: rUbiq(dig)"no-lysines" [substitution]
    *** unaffected by: rUbiq(K48R) [substitution]
    *** source: 12867425-Fig-3i

================================================================
Nemo features required for Nemo ubiquitination by Ube2d3 + Ciap1
================================================================

  *** rNemo[tAb] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** comment: substitution with xNemo(del(393-419)) caused a pattern change
    *** comment: substitution with xNemo(C417R) caused a pattern change
    *** source: 12867425-Fig-3d

================================================================
Ciap1 features required for Nemo ubiquitination by Ube2d3 + Ciap1
================================================================

  *** rNemo[tAb] upshift[WBMS] is increased by (rUbiq + rUbe1 + rUbe2d3 + rCiap1)
    *** cells: none
    *** inhibited by: rCiap1(1-570)"del-RING" [substitution]
    *** inhibited by: rCiap1(C605A) [substitution]
    *** unaffected by: xCiap1(562-618)"RING" [substitution]
    *** source: 12867425-Fig-3h

===========================================
Nemo ubiquitination is increased itpo Ciap1
===========================================

  *** xNemo[tAb]IP ubiq[tUbAb] is increased itpo xCiap1
    *** cells: HEK293T<xUbiq> in BMS
    *** inhibited by: xCiap1(1-570)"del-RING" [substitution]
    *** inhibited by: xCiap1(562-618)"RING" [substitution]
    *** source: 12867425-Fig-3b

  *** xNemo[tAb]IP ubiq[tUbAb] is increased itpo xUbiq
    *** cells: HEK293T in BMS
    *** enhanced by: xCiap1 [addition]
    *** unaffected by: xXiap [addition]
    *** unaffected by: xTraf2 [addition]
    *** source: 12867425-Fig-3a
  *** xNemo[tAb] copptby[WB] xCiap1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 12867425-Fig-4c











