=====================
Irak4 kinase activity
=====================

requires K213/K214 (ATP binding site)

  *** xIrak4[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xIrak4(K213A/K214A)"KD" [substitution]
    *** source: 15767370-Fig-2b
  *** xIrak4[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xIrak4(K213A/K214A)"KD" [substitution]
    *** source: 17141195-Fig-2a

 --- requires T345/S346 (phos sites)

  *** xIrak4[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: HEK293T in BMS
    *** partially inhibited by: xIrak4(T342A) [substitution]
    *** partially inhibited by: xIrak4(T345A) [substitution]
    *** partially inhibited by: xIrak4(S346A) [substitution]
    *** inhibited by: xIrak4(T345A/S346A) [substitution]
    *** inhibited by: xIrak4(T342A/T345A/S346A) [substitution]
    *** source: 17141195-Fig-2a

====================
AutopPhosphorylation
====================

  *** rIrak4[32P] phos[32P-ATP] is detectable
    *** cells: none
    *** inhibited by: rIrak4(K213A/K214A)"KD" [substitution]
    *** source: 11960013-Fig-5a
  *** rIrak4[32P] phos[32P-ATP] is detectable
    *** cells: none
    *** inhibited by: rIrak4(K213A/K214A)"KD" [substitution]
    *** source: 12860405-Fig-2a
  *** xIrak4[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: COS7 in BMS
    *** inhibited by: xIrak4(K213A/K214A) [substitution]
    *** inhibited by: xIrak4(1-185) [substitution]
    *** source: 19181383-Fig-5

on T342/T345/T346

  *** rIrak4[32P] phos[32P-ATP] is detectable
    *** cells: none
    *** partially inhibited by: rIrak4(T342A) [substitution]
    *** partially inhibited by: rIrak4(T345A) [substitution]
    *** partially inhibited by: rIrak4(S346A) [substitution]
    *** inhibited by: rIrak4(T342A/T345A) [substitution]
    *** inhibited by: xIrak4(T345A/S346A) [substitution]
    *** source: 17141195-Fig-2a

================
coprecipitations
================

Myd88--
  *** xIrak4(K213A/K214A)"KD"[tAb] copptby[WB] xMyd88[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xIrak4(WT) [substitution]
    *** source: 11960013-Fig-2a
Traf6--
  *** xIrak4(K213A/K214A)"KD"[tAb] copptby[WB] xTraf6[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xIrak4(WT) [substitution]
    *** source: 11960013-Fig-2b
Peli2--
  *** xIrak4[tAb] copptby[WB] xPeli2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** enhanced by: xIrak4(K213A/K214A)"KD" [substitution]
    *** source: 12860405-Fig-1a


