===============
Kinase Activity
===============

Tsc2--

  *** xRaf1[tAb]IP IVKA(Mek1)(S217/S222)[phosAb] is increased irt Serum (10 min)
    *** cells: HEK293 in BMLS
    *** reqs: Tsc2 [RNAi]
    *** source: 16803888-Fig-1d
  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Serum (10 min)
    *** cells: HEK293 in BMLS
    *** reqs: Tsc2 [RNAi]
    *** source: 16803888-Fig-1d

Rheb-GDP--

  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Serum (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: xRheb(Q64L) [addition]
    *** source: 16803888-Fig-1c
  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Serum (10 min)
    *** cells: HEK293 in BMLS
    *** partially inhibited by: xRheb [addition]
    *** source: 16803888-Fig-1b

Chemicals--

  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Serum (10 min)
    *** cells: HEK293 in BMLS
    *** unaffected by: Rapamycin [addition]
    *** source: 16803888-Fig-1b

no extras--

  *** xRaf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Serum (times)
    *** cells: CHO in BMS
    *** times: 0 5++ 10+ 15+ 30+ min
    *** source: 8120027-Fig-5b
  *** xRaf1[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Serum (tnr)
    *** cells: HEK293<xRaf1> in BMLS
    *** source: 11325826-Fig-1b

===============
Phosphorylation
===============

phos(S43)

  *** Raf1[Ab]IP phos(S43)[2D-PPMap] is increased irt Serum (5 min)
    *** cells: NIH3T3 in BMLS
    *** source: 11782426-Fig-1b

phos(S259)

  *** Raf1[Ab]IP phos(S259)[2D-PPMap] is decreased irt Serum (5 min)
    *** cells: NIH3T3 in BMLS
    *** source: 11782426-Fig-1b
  *** Raf1[Ab]IP phos(S259)[phosAb] is decreased irt Serum (times)
    *** cells: COS1 in BMS
    *** times: 0++ 2+ 5+ min
    *** source: 11782426-Fig-1c

phos(S338)

  *** Raf1[Ab]IP phos(S338)[phosAb] is increased irt Serum (10 min)
    *** cells: HEK293 in BMLS
    *** partially inhibited by: xRheb [addition]
    *** unaffected by: Rapamycin [addition]
    *** source: 16803888-Fig-1a
  *** Raf1[Ab]IP phos(S338)[phosAb] is increased irt Serum (times)
    *** cells: COS1 in BMS
    *** times: 0 2+ 5++ min
    *** source: 11782426-Fig-1c

phos(S621)

  *** Raf1[Ab]IP phos(S621)[2D-PPMap] is decreased irt Serum (5 min)
    *** cells: NIH3T3 in BMLS
    *** source: 11782426-Fig-1b
  *** xRaf1[tAb]IP infraction(membrane)[WB] is increased irt Serum (times)
    *** cells: CHO in BMLS
    *** times: 0 5++ 10+ 15+ 30+ min
    *** source: 8120027-Fig-5a
  *** xRaf1[tAb]IP phos(S338)[phosAb] is increased irt Serum (10 min)
    *** cells: HEK293 in BMLS
    *** partially inhibited by: xRheb [addition]
    *** unaffected by: Rapamycin [addition]
    *** source: 16803888-Fig-1b

