==================================================
Smad3 relocation to nucleus is inhibited by xSara
==================================================

  *** xSmad3[XFP] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: COS1 in BMS
    *** partially inhibited by: xSara [addition]
    *** source: 15356634-Fig-S4

========================================
Smad3 relocation to nucleus requires Pml
========================================

  *** Smad3[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (1 hr)
    *** cells: mEFs in BMS
    *** reqs: Pml [KO]
    *** source: 15356634-Fig-S2f
  *** Smad3[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: mEFs in BMS
    *** partially reqs: Pml [KO]
    *** source: 15356634-Fig-S2g
  *** xSmad3[tAb] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: mEFs in BMS
    *** reqs: Pml [KO]
    *** source: 15356634-Fig-S2h

=========================================
Smad3 in nucleus is phosed on S423/S425
=========================================

  *** Smad3[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** comment: Smad3 phos(S423/S425) is located in nuclear fraction
    *** does not req: Ppm1a [RNAi]
    *** source: 16751101-Fig-4d

=============================================
Smad3 relocation to nucleus requires Jnks
but not phosphorylation on S422/S423/S425
=============================================

  *** xSmad3[tAb] locatedin(nucleus)[IHC] is increased irt Tgfb1 (30 min)
    *** cells: MV1LU in BMLS
    *** inhibited by: xJnk?(mnr)"DN" [addition]
    *** source: 10601313-Fig-4c
  *** xSmad3(S422A/S423A/S425A)[tAb] locatedin(nucleus)[IHC] is increased irt Tgfb1 (tnr)
    *** cells: MV1LU in BMLS
    *** inhibited by: xJnk?(mnr)"DN" [addition]
    *** enhanced by: xJnk? [addition]
    *** source: 10601313-Fig-5b

=================================================================
Smad3 relocation to nucleus requires phosphorylation on S416/S418
=================================================================

  *** xSmad3[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: CHO<xSmad3> in BMLS
    *** inhibited by: xSmad3(S416A/P417A/S418A) [substitution]
    *** source: 18760948-Fig-7b
  *** xSmad3(mnr)"MH2"[tAb] infraction(nuclear)[WB] is detectable
    *** cells: COS7<xSmad3(mnr)"MH2"> in BMLS
    *** inhibited by: xSmad3(mnr/S416A/P417A/S418A)"MH2" [substitution]
    *** unaffected by: xSmad3(mnr/S416D/S418D)"MH2" [substitution]
    *** source: 18760948-Fig-7d
  *** xSmad3(mnr)"MH2"[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: CHO<xSmad3(mnr)"MH2"> in BMLS
    *** inhibited by: xSmad3(mnr/S416A/P417A/S418A)"MH2" [substitution]
    *** enhanced by: xSmad3(mnr/S416D/S418D)"MH2" [substitution]
    *** source: 18760948-Fig-7c


==================================================
Smad3 relocation to nucleus does not require Smad4
==================================================

  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: BXPC3<Smad4~null> in BMS "treated with CHX"
    *** times: 0 60++ 90++ 120++ 180++ 240+ min
    *** source: 12191474-Fig-5a

==================================================================
Smad3 relocation to nucleus does not require new protein synthesis
==================================================================

  *** Smad3[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS "nuclear-extracts"
    *** times: 0 1 5 10+ 15+ 20++ 30++ 45++ 60++ 120++ 180+ 240+ 300+ 360 420 480 min
    *** comment: decrease in signal after 120 min is faster itpo of CHX
    *** source: 12191474-Fig-1a
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (60 min)
    *** cells: HACAT in BMS "treated with CHX"
    *** source: 12191474-Fig-5c
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (60 min)
    *** cells: HACAT in BMS "treated with CHX"
    *** unaffected by: NaN3 + 2DOG [addition]
    *** source: 12191474-Fig-6
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS "treated with CHX"
    *** times: 0 60++ 90++ 120++ 180++ 240+ min
    *** source: 12191474-Fig-3b
  *** Smad3[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS "treated with CHX"
    *** times: 0 30++ 60++ 120++ 180++ 360+ 540 min
    *** source: 11074002-Fig-7a

===========================
inhibition itpo of Hras-GTP
===========================

  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (30 min)
    *** cells: EPH4 in BMS
    *** inhibited by: xHras(mnr)"vRas" [addition]
    *** source: 10197981-Fig-2d
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (30 min)
    *** cells: MV1LU in BMS
    *** inhibited by: Egf (30 min) [pretreatment]
    *** source: 10197981-Fig-6a
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: EPH4 in BMS
    *** times: 0 30++ 60++ 180++ 240+ min
    *** inhibited by: xHras(mnr)"vRas" [addition]
    *** source: 10197981-Fig-2e

  *** Smad3[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb2
    *** cells: AKR2B in BMLS
    *** unaffected by: Hras(S17N)"DN" [addition]
    *** source: 17440079-Fig-3b

============================
inhibition itpo of Pdpk1-act
============================

  *** xSmad3[tAb] locatedin(nucleus)[IHC] is increased irt Tgfb1 (tnr)
    *** cells: HEP3B in BMS
    *** inhibited by: xPdpk1 [addition]
    *** unaffected by: xPdpk1(mnr)"KD" [addition]
    *** repressed by: Pdpk1 [RNAi]
    *** source: 17327236-Fig-9a

=====
Other
=====

  *** Smad3[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: MV1LU in BMS
    *** source: 9311995-Fig-7
  *** Smad3[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** source: 12150994-Fig-5a
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (30 min)
    *** cells: HELA in BMS
    *** source: 10934479-Fig-3a
  *** xSmad3[XFP] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: COS1<xSara><xPmlc> in BMS
    *** source: 15356634-Fig-2g


chemicals

  *** Smad3[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (tnr)
    *** cells: AKR2B in BMLS
    *** unaffected by: LY294002 [pretreatment]
    *** source: 16288034-Fig-2b


