Find out what happens at shorter times - different doses

===============
Phosphorylation
===============

S15

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt UV (12 hr)
    *** cells: A549 in BMS
    *** partially inhibited by: SB203580 [pretreatment]
    *** partially inhibited by: SB202190 [pretreatment]
    *** source: 10581258-Fig-6b
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt UV (12 hr)
    *** cells: U2OS in BMS
    *** comment: increase correlates with the increase in Tp53 prot-exp
    *** source: 17906639-Fig-S1a
  *** xTp53[phosAb] phos(S15)[phosAb] is increased irt UV (times)
    *** cells: HCT116<Tp53~null><xTp53> in BMS
    *** times: 0+ 4++ 8++ hr
    *** unaffected by: xTp53(S33A) [substitution]
    *** unaffected by: xTp53(S46A) [substitution]
    *** partially inhibited by: xTp53(S33A/S46A) [substitution]
    *** source: 10581258-Fig-5b
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt UV (tnr)
    *** cells: U2OS in BMS
    *** source: 12397362-Fig-1a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt UV (4 hr)
    *** cells: U2OS in BMS
    *** unaffected by: xE4f1 [addition]
    *** source: 17110336-Fig-S6e
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt UV (times)
    *** cells: 1070SK in BMS
    *** times: 0 1 5+ hr
    *** source: 12556884-Fig-2d

S33

  *** Tp53[phosAb] phos(S33)[phosAb] is increased irt UV (12 hr)
    *** cells: A549 in BMS
    *** partially inhibited by: SB203580 [pretreatment]
    *** partially inhibited by: SB202190 [pretreatment]
    *** source: 10581258-Fig-6b
  *** xTp53[phosAb] phos(S33)[phosAb] is increased irt UV (times)
    *** cells: HCT116<Tp53~null><xTp53> in BMS
    *** times: 0+ 4++ 8+++ hr
    *** unaffected by: xTp53(S46A) [substitution]
    *** source: 10581258-Fig-5b

S37

  *** xTp53[phosAb] phos(S37)[phosAb] is increased irt UV (times)
    *** cells: HCT116<Tp53~null><xTp53> in BMS
    *** times: 0+ 4++ 8++ hr
    *** inhibited by: xTp53(S33A) [substitution]
    *** unaffected by: xTp53(S46A) [substitution]
    *** inhibited by: xTp53(S33A/S46A) [substitution]
    *** source: 10581258-Fig-5b

S46

  *** Tp53[phosAb] phos(S46)[phosAb] is increased irt UV (times)
    *** cells: MCF7 in BMS
    *** times: 0 1 6+ hr
    *** source: 11740489-Fig-5e
  *** Tp53[Ab] prot-exp[WB] is increased irt UV (12 hr)
    *** cells: MCF7 in BMS
    *** comment: new Tp53 protein is phosed on S46 [phosAb]
    *** source: 11780126-Fig-5a

T81

  *** Tp53[phosAb] phos(T81)[phosAb] is increased irt UV (2 hr)
    *** cells: MCF7 in BMS
    *** reqs: Jnk1 and/or Jnk2 [AntiSense]
    *** source: 11283254-Fig-5c
  *** Tp53[phosAb] phos(T81)[phosAb] is increased irt UV (times)
    *** cells: hFibros in BMS
    *** times: 0+ 1++ 2++ 4++ 8++ 24++ hr
    *** source: 11283254-Fig-2a

==============
Ubiquitination
==============

  *** Tp53[Ab] ubiq[tUbAb-ppt] is decreased irt UV (times)
    *** cells: MCF7<xUbiq> in BMS
    *** times: 0+ 4 8 hr
    *** source: 15242646-Fig-7a
  *** xTp53[Ab] ubiq[tUbAb-ppt] is decreased irt UV (4 hr)
    *** cells: U2OS<xTp53><xUbiq> in BMS
    *** source: 17110336-Fig-S6b
  *** xTp53[Ab] ubiq[tUbAb-ppt] is unchanged irt UV (4 hr)
    *** cells: U2OS<xTp53><xUbiq> in BMS
    *** source: 17110336-Fig-S6b
  *** Tp53[Ab] ubiq[tUbAb] is decreased irt UV (4 hr)
    *** cells: U2OS<xUbiq> in BMS
    *** source: 17110336-Fig-7a

===========
Neddylation
===========

  *** Tp53[Ab] nedd[tNdAb-ppt] is increased irt UV (times)
    *** cells: MCF7<xNedd8> in BMS
    *** times: 0 4+ 8 hr
    *** comment: only one Tp53-Nedd8 band was seen around 75 kD
    *** source: 15242646-Fig-7a

=====================
K373/K382 Acetylation
=====================

E4f1--

  *** Tp53[Ab]IP acetyl(K373)[AcAb] is detectable
    *** cells: U2OS in BMS "treated with UV for 4 hr"
    *** partially reqs: E4f1 [RNAi]
    *** source: 17110336-Fig-7c
  *** Tp53[Ab]IP acetyl(K373)[AcAb] is increased irt UV (4 hr)
    *** cells: U2OS in BMS
    *** unaffected by: xE4f1(del(41-85)) [addition]
    *** source: 17110336-Fig-7b

Pin1--

  *** xTp53{acetyl(K373/K382)}[AcAb] boundto(Cdkn1a-gene)[ChIP] is increased irt UV (12 hr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** comment: normalized for Tp53 prot but not acetyl
    *** reqs: Pin1 [RNAi]
    *** inhibited by: PIB [pretreatment]
    *** source: 17906639-Fig-5c
  *** Tp53[AcAb] acetyl(K373)[AcAb] is increased irt UV (12 hr)
    *** cells: HCT116 in BMS
    *** reqs: Pin1 [RNAi]
    *** source: 17906639-Fig-4d
  *** Tp53[AcAb] acetyl(K382)[AcAb] is increased irt UV (12 hr)
    *** cells: HCT116 in BMS
    *** reqs: Pin1 [RNAi]
    *** source: 17906639-Fig-4d

Tp53 features

  *** xTp53{acetyl(K373/K382)}[AcAb] boundto(Cdkn1a-gene)[ChIP] is increased irt UV (12 hr)
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** comment: not normalized for Tp53 prot or acetyl
    *** partially inhibited by: xTp53(T81A) [substitution]
    *** source: 17906639-Fig-6b
  *** Tp53[phosAb] acetyl(K382)[AcAb] is increased irt UV (12 hr)
    *** cells: U2OS in BMS
    *** comment: increase correlates with the increase in Tp53 prot-exp
    *** source: 17906639-Fig-S1a
  *** Tp53[Ab] acetyl(K373)[AcAb-ppt] is increased irt UV (4 hr)
    *** cells: U2OS in BMS
    *** source: 9891054-Fig-7c

================
K320 Acetylation
================

Inhibited by E4f1--

  *** Tp53[Ab]IP acetyl(K320)[AcAb] is increased irt UV (4 hr)
    *** cells: U2OS in BMS
    *** inhibited by: xE4f1 [addition]
    *** unaffected by: xE4f1(del(41-85)) [addition]
    *** source: 17110336-Fig-7b
  *** Tp53[Ab]IP acetyl(K320)[AcAb] is increased itao E4f1 [RNAi]
    *** cells: U2OS in BMS "treated with UV for 4 hr"
    *** source: 17110336-Fig-7c


  *** Tp53[Ab]IP acetyl(K320)[AcAb-ppt] is increased irt UV (4 hr)
    *** cells: U2OS in BMS
    *** source: 9891054-Fig-7c
  *** Tp53[Ab]IP acetyl(K320)[AcAb] is increased irt UV (4 hr)
    *** cells: U2OS in BMS
    *** source: 17110336-Fig-7a
  *** Tp53[Ab]IP acetyl(K320)[AcAb] is increased irt UV (tnr)
    *** cells: U2OS in BMS
    *** source: 17110336-Fig-S6b

=================
Protein Stability
=================

  *** xTp53[Ab]IP prot-stability[35S-pulse-chase] is increased irt UV (2 hr)
    *** cells: HCT116<Tp53~null><xTp53> in BMS
    *** source: 10581258-Fig-3c
  *** xTp53[Ab]IP prot-stability[35S-pulse-chase] is increased irt UV (5 hr)
    *** cells: mEFs<Tp53~null><xTp53> in BMS
    *** inhibited by: xTp53(T81A) [substitution]
    *** source: 11283254-Fig-3
  *** xTp53[Ab]IP prot-stability[35S-pulse-chase] is increased irt UV (12 hr)
    *** cells: A549 in BMS
    *** unaffected by: SB202190 [pretreatment]
    *** source: 10581258-Fig-6c
  *** xTp53(S33A/S46A)[Ab]IP prot-stability[35S-pulse-chase] is increased irt UV (2 hr)
    *** cells: HCT116<Tp53~null><xTp53> in BMS
    *** source: 10581258-Fig-4b
  *** Tp53[Ab] prot-stability[CHX-WB] is increased irt UV (12 hr)
    *** cells: WS1 in BMS
    *** source: 15029243-Fig-3c

==================
Protein Expression
==================

Jnk1-- or Jnk2--  ???

  *** Tp53[Ab] prot-exp[WB] is increased irt UV (2 hr)
    *** cells: MCF7 in BMS
    *** does not req: Jnk1 or Jnk2 [AntiSense]
    *** source: 11283254-Fig-5c
  *** Tp53[Ab]IP prot-exp[WB] is increased irt UV (4 hr)
    *** cells: JB6CL41 in BMLS
    *** comment: Tp53 is phosed on S20 [phosAb]
    *** partially inhibited by: xJnk1(mnr)"DN" [addition]
    *** source: 11896587-Fig-2b

Erks--

  *** Tp53[Ab]IP prot-exp[WB] is increased irt UV (4 hr)
    *** cells: JB6CL41 in BMLS
    *** comment: Tp53 is phosed on S20 [phosAb]
    *** partially inhibited by: xErk2(mnr)"DN" [addition]
    *** source: 11896587-Fig-2b

P38s--

  *** Tp53[Ab]IP prot-exp[WB] is increased irt UV (4 hr)
    *** cells: JB6CL41 in BMLS
    *** comment: Tp53 is phosed on S20 [phosAb]
    *** partially inhibited by: xP38?(mnr)"DN" [addition]
    *** source: 11896587-Fig-2b

Pin1--

  *** Tp53[Ab] prot-exp[WB] is increased irt UV (16 hr)
    *** cells: mEFs in BMS
    *** partially reqs: Pin1 [KO]
    *** source: 12397361-Fig-S2d
  *** Tp53[Ab] prot-exp[WB] is increased irt UV (tnr)
    *** cells: U2OS in BMS
    *** enhanced by: xPin1 [addition]
    *** source: 12397361-Fig-3a
  *** Tp53[Ab] prot-exp[WB] is increased irt UV (tnr)
    *** cells: mEFs in BMS
    *** partially reqs: Pin1 [KO]
    *** comment: new Tp53 is phosed on S15 regardless of the presence of Pin1
    *** source: 12397362-Fig-3a
  *** Tp53[Ab] prot-exp[WB] is increased irt UV (tnr)
    *** cells: mEFs<Pin1~null><xPin1> in BMS
    *** reqs: xPin1 [omission]
    *** inhibited by: xPin1(C109A)"CIA" [substitution]
    *** source: 12397362-Fig-3b

  *** Tp53[Ab]IP prot-exp[WB] is increased irt UV (16 hr)
    *** cells: LNCAP in BMS
    *** comment: Tp53IP is phosed on S15 [phosAb]
    *** source: 9363941-Fig-2e
  *** xTp53[Ab] prot-exp[WB] is increased irt UV (5 hr)
    *** cells: MCF7<xTp53> in BMS
    *** source: 10673501-Fig-4b
  *** xTp53[Ab] prot-exp[WB] is increased irt UV (5 hr)
    *** cells: MCF7<xTp53><xChek1> in BMS
    *** source: 10673501-Fig-4b
  *** Tp53[Ab] prot-exp[WB] is increased irt UV (times)
    *** cells: MCF7 in BMS
    *** times: 0 7 15 30 60 120+ 240++ 480+++ 720+++ 1440++ min
    *** comment: new Tp53 protein is phosed on S15 [phosAb]
    *** comment: new Tp53 protein is phosed on S20 [phosAb]
    *** comment: new Tp53 protein is phosed on S26 [phosAb]
    *** source: 11030628-Fig-5a
  *** xTp53[Ab] prot-exp[WB] is increased irt UV (tnr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** comment: xTp54 is phosed on T81 and S15 
    *** source: 11283254-Fig-5a
  *** Tp53[Ab] prot-exp[WB] is increased irt UV (tnr)
    *** cells: U2OS in BMS
    *** source: 12397362-Fig-1a
  *** Tp53[Ab] prot-exp[WB] is increased irt UV (12 hr)
    *** cells: MCF7 in BMS
    *** source: 11780126-Fig-5c
  *** Tp53[Ab] prot-exp[WB] is increased irt UV (times)
    *** cells: MCF7 in BMS
    *** times: 0 3 6+ 9+ hr
    *** unaffected by: MG132 [cotreatment]
    *** source: 12963717-Fig-1g
  *** Tp53[Ab] prot-exp[WB] is increased irt UV (times)
    *** cells: MCF7 in BMS
    *** times: 0 3+ 6+ 9+ hr
    *** unaffected by: MG132 [cotreatment]
    *** source: 12963717-Fig-1g
  *** Tp53[Ab]IP prot-exp[WB] is increased irt UV (times)
    *** cells: JB6CL41 in BMLS
    *** times: 0+ 1+ 2++ 4+++ 6+++ hr
    *** comment: Tp53 is phosed on S20 [phosAb]
    *** source: 11896587-Fig-1

=================
Reporter Activity
=================

  *** Tp53-reporter[Luc] is increased irt UV (6 hr)
    *** cells: mEFs<Tp53~null><xTp53> in BMS
    *** source: 11283254-Fig-4b
  *** Tp53-reporter[Luc] is increased irt UV (tnr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** inhibited by: xTp53(T81A) [substitution]
    *** source: 11283254-Fig-4a
  *** Tp53-reporter[Luc] is increased irt UV (tnr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** source: 11283254-Fig-5c
  *** Tp53-reporter[Luc] is increased irt UV (times)
    *** cells: mEFs in BMS
    *** times: 0 6++ 12+ hr
    *** reqs: Jnk1 [KO]
    *** reqs: Jnk2 [KO]
    *** source: 11896587-Fig-6a

===================
Binding to Bax-gene
===================

  *** Tp53[Ab] boundto(Bax-gene)[ChIP] is increased irt UV (12 hr)
    *** cells: HCT116 in BMS "treated with Nutlin3a"
    *** partially reqs: Pin1 [RNAi]
    *** source: 17906639-Fig-1c
  *** Tp53[Ab] boundto(Bax-gene)[ChIP] is increased irt UV (times)
    *** cells: HCT116 in BMS
    *** times: 0 5 8+ 12++ hr
    *** comment: not normalized for increased Tp53 prot-exp
    *** source: 17906639-Fig-2

======================
Binding to Cdkn1a-gene
======================

  *** Tp53[Ab] boundto(Cdkn1a-gene)[ChIP] is increased irt UV (12 hr)
    *** cells: HCT116 in BMS
    *** comment: both total and bound Tp53 is decreased itao Pin1 [RNAi]
    *** source: 17906639-Fig-1b
  *** Tp53[Ab] boundto(Cdkn1a-gene)[ChIP] is increased irt UV (12 hr)
    *** cells: HCT116 in BMS "treated with Nutlin3a"
    *** reqs: Pin1 [RNAi]
    *** source: 17906639-Fig-1c
  *** Tp53[Ab] boundto(Cdkn1a-gene)[ChIP] is increased irt UV (12 hr)
    *** cells: U2OS in BMS
    *** comment: increase correlates with the increase in Tp53 prot-exp
    *** source: 17906639-Fig-S1a
  *** Tp53[Ab] boundto(Cdkn1a-gene)[ChIP] is increased irt UV (times)
    *** cells: HCT116 in BMS
    *** times: 0 5+ 8++ 12++ hr
    *** comment: not normalized for increased Tp53 prot-exp
    *** source: 17906639-Fig-2




  *** Tp53[Ab] copptby[WB] Pin1[Ab] is increased irt UV (tnr)
    *** cells: HEK293 in BMS
    *** source: 12397362-Fig-1e
  *** Tp53[Ab] copptby[WB] Pin1[Ab] is increased irt UV (tnr)
    *** cells: MCF7 in BMS
    *** source: 12397361-Fig-S1a
  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is increased irt UV (tnr)
    *** cells: U2OS in BMS
    *** comment: snagged Tp53 is phosed on S15 [phosAb]
    *** source: 12397362-Fig-1a



