========================================
Coprecipitation with IL1R1 and/or IL1Rap
========================================

IL1Rap features--

  *** Irak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1b (5 min)
    *** cells: HELA in BMS
    *** partially inhibited by: xIL1Rap(1-403) [addition]
    *** source: 9371760-Fig-4a
  *** Irak1[Ab] copptby[WB] xIL1Rap[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xIL1Rap(1-403) [substitution]
    *** source: 9371760-Fig-3c

inhibited by IL1rn--

  *** IL1R1[Ab] copptby[WB] Irak1[Ab] is increased irt IL1a (times)
    *** cells: MRC5 in BMLS
    *** times: 0 5+ 10+ 20+ min
    *** inhibited by: IL1rn (tnr) [stim]
    *** source: 9261174-Fig-2b

not Irak1-- kinase actvity

  *** xIrak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1b (5 min)
    *** cells: EL4<xIrak1> in BMS "post-nuclear-extracts"
    *** unaffected by: xIrak1(K239S)"KD" [substitution]
    *** source: 15896328-Fig-3a
  *** xIrak1(K239S)"KD"[Ab] copptby[WB] (xIL1R1[tAb] + xIL1Rap[tAb]) is detectable
    *** cells: HEK293<xMyd88> in BMS
    *** inhibited by: xIrak1(WT) [substitution]
    *** source: 9430229-Fig-5c

Irak4-- kinase activitity

  *** IL1R1[Ab] copptby[WB] Irak1[Ab] is increased irt IL1b (times)
    *** cells: hFibros<Irak4~null> in BMS
    *** times: 0 30+ 60+ min
    *** enhanced by: xIrak4 [addition]
    *** source: 15084582-Fig-7b
  *** Irak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 1++ 2++ 5+ 10 20 30 min
    *** reqs: Irak4 [KO]
    *** source: 15292196-Fig-2a
  *** Irak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 5+ 10+ 15+ min
    *** inhibited by: kIrak4(K213A/K214A)"KD" [substitution]
    *** source: 17337443-Fig-2b

Myd88--  ???

  *** Irak1[Ab] copptby[WB] xIL1R1[Ab] is increased irt IL1b (5 min)
    *** cells: HEK293 in BMS
    *** partially inhibited by: xMyd88 [addition]
    *** partially inhibited by: xMyd88(1-151)"Nterm" [addition]
    *** partially inhibited by: xMyd88(152-296)"Cterm" [addition]
    *** source: 9430229-Fig-5b
  *** Irak1[Ab] copptby[WB] xIL1R1[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1><xIL1Rap> in BMS
    *** times: 0 1+ 2+ 5 10 min
    *** inhibited by: xMyd88 [addition]
    *** source: 9430229-Fig-3a
  *** xIrak1(K239S)"KD"[Ab] copptby[WB] (xIL1R1[tAb] + xIL1Rap[tAb]) is detectable
    *** cells: HEK293<xMyd88> in BMS
    *** reqs: xMyd88 [omission]
    *** source: 9430229-Fig-5c
  *** xIrak1(T66A)[Ab] copptby[WB] (xIL1R1[tAb] + xIL1Rap[tAb]) is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xMyd88 [addition]
    *** source: 10854325-Fig-4c

Tollip--

  *** xIrak1(D340N)"KD"[Ab] copptby[WB] (xIL1R1[tAb] + xIL1Rap[tAb]) is detectable
    *** cells: HEK293T<xTollip> in BMS
    *** reqs: xTollip [omission]
    *** source: 10854325-Fig-4a
  *** xIrak1(T66A)[Ab] copptby[WB] (xIL1R1[tAb] + xIL1Rap[tAb]) is detectable
    *** cells: HEK293T<xTollip> in BMS
    *** reqs: xTollip [omission]
    *** source: 10854325-Fig-4a
  *** xIrak1(D340N)"KD"[Ab] copptby[WB] (xIL1R1(mnr)"Myd88-binding-mut"[tAb] + xIL1Rap(mnr)"Myd88-binding-mut"[tAb]) is detectable
    *** cells: HEK293T<xTollip> in BMS
    *** reqs: xTollip [omission]
    *** source: 10854325-Fig-4b

not Traf2--

  *** Irak1[Ab] copptby[WB] xIL1R1[tAb] is increased irt IL1 (5 min)
    *** cells: HEK293<xIL1R1> in BMS
    *** unaffected by: xTraf2(87-501) [addition]
    *** source: 8837778-Fig-4b

not Traf6--

  *** Irak1[Ab] copptby[WB] xIL1R1[tAb] is increased irt IL1 (5 min)
    *** cells: HEK293<xIL1R1> in BMS
    *** unaffected by: xTraf6(87-502) [addition]
    *** source: 8837778-Fig-4b

chemicals

  *** IL1R1[Ab] copptby[WB] Irak1[Ab] is increased irt IL1a (times)
    *** cells: MRC5 in BMLS
    *** times: 0 2+ 5+ 10+ 20+ 40+ min
    *** unaffected by: LLL [addition]
    *** unaffected by: IEAL [addition]
    *** unaffected by: LLNV [addition]
    *** source: 9261174-Fig-5a
  *** IL1R1[Ab] copptby[WB] Irak1[Ab] is increased irt IL1a (times)
    *** cells: MRC5 in BMLS
    *** times: 0 2 5+ 10++ 20++ min
    *** inhibited by: K252a [addition]
    *** source: 9261174-Fig-6
  *** xIrak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1b (5 min)
    *** cells: EL4<xIrak1> in BMS "post-nuclear-extracts"
    *** inhibited by: Menadione [addition]
    *** source: 15896328-Fig-3b







===================================================
copreciptitation with other IL1R1 binding proteins
===================================================

Irak4--

  *** Irak1[Ab] copptby[WB] Irak4[Ab] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0+ 5++ 15+ min
    *** comment: coppt Irak1 is upshifted [WBMS]
    *** inhibited by: kIrak4(K213A/K214A)"KD" [substitution]
    *** source: 17337443-Fig-2a
  *** Irak1[Ab] copptby[WB] Irak4[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 2+ 10++ 30+ min
    *** source: 11960013-Fig-2c
  *** Irak1[Ab] copptby[WB] Irak4[Ab] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 10+ 20+ min
    *** reqs: Myd88 [KO]
    *** source: 15084582(D)

Traf6--

  *** Irak1[Ab] copptby[WB] Traf6[Ab] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 10+ 30+ 60+ min
    *** source: 12496252-Fig-2b

=========
no extras
=========

  *** Irak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1b (5 min)
    *** cells: HELA in BMS
    *** source: 9371760-Fig-3a
  *** Irak1[Ab] copptby[WB] IL1Rap[Ab] is increased irt IL1b (5 min)
    *** cells: HEK293<xIL1R1> in BMS
    *** source: 9371760-Fig-3a
  *** Irak1[Ab] copptby[WB] IL1Rap[Ab] is increased irt IL1b (5 min)
    *** cells: HELA in BMS
    *** source: 9371760-Fig-3a
  *** Irak1[Ab] copptby[WB] xIL1R1[Ab] is increased irt IL1b (5 min)
    *** cells: HEK293 in BMS
    *** source: 9371760-Fig-3a
  *** Irak1[Ab] copptby[WB] xIL1R1[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** source: 9371760-Fig-3b
  *** Irak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1a (times)
    *** cells: EL4 in BMS
    *** times: 0 1++ 2+++ 3++ 4+ 5++ 10 20 30+ 60 min
    *** source: 17507369-Fig-6
  *** Irak1[Ab] copptby[WB] xIL1R1[Ab] is increased irt IL1 (3 min)
    *** cells: HEK293 in BMS
    *** source: 8599092-Fig-4a
  *** Irak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1 (3 min)
    *** cells: HELA in BMS
    *** source: 8599092-Fig-4b
  *** Irak1[Ab] copptby[WB] xIL1R1[Ab] is increased irt IL1 (times)
    *** cells: HEK293 in BMS
    *** times: 0 1+ 2++ 5++ 10++ 20++ min
    *** comment: the mobility of Irak1 decreases as time increases
    *** source: 8599092-Fig-4c
  *** Irak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1b (times)
    *** cells: EL4 in BMS
    *** times: 0 2+ 5 10 min
    *** source: 10854325-Fig-2b
  *** Irak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1b (10 min)
    *** cells: HEK293 in BMS
    *** source: 12242293-Fig-2c
  *** Irak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 1+ 2++ 5++ 10++ 30+++ 60++ 120++ 240+ min
    *** source: 12242293-Fig-2a
  *** Irak1[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1a (times)
    *** cells: MRC5 in BMLS
    *** times: 0 1++ 2++ 4+ 10+ min
    *** source: 9261174-Fig-2a
  *** IL1R1[Ab] copptby[WB] Irak1[Ab] is increased irt IL1a (times)
    *** cells: MRC5 in BMLS
    *** times: 0 1+ 2+ 4++ 10++ min
    *** source: 9261174-Fig-2a
  *** Irak1[Ab] copptby[WB] IL1R1[tAb] is increased irt IL1b (times)
    *** cells: HEK293<xTLR3> in BMS
    *** times: 0 10+ 20++ min
    *** source: 12609980-Fig-2a

===========
Basal State
===========

Irak1-- features

  *** xIrak1(1-523)[Ab] copptby[WB] IL1R1[Ab] is undetectable
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** unaffected by: IL1b (10 min) [stim]
    *** source: 12242293-Fig-2c
  *** xIrak1(1-523)[Ab] copptby[WB] IL1R1[Ab] is undetectable
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** unaffected by: IL1b (10 min) [stim]
    *** source: 12242293-Fig-4c
  *** xIrak1(1-619)[Ab] copptby[WB] IL1R1[Ab] is detectable
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** enhanced by: IL1b (10 min) [stim]
    *** source: 12242293-Fig-2c
  *** xIrak1(1-619)[Ab] copptby[WB] Tak1[Ab] is detectable
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** enhanced by: IL1b (10 min) [stim]
    *** source: 12242293-Fig-4c
  *** xIrak1(104-712)[Ab] copptby[WB] IL1R1[Ab] is undetectable
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** unaffected by: IL1b (10 min) [stim]
    *** source: 12242293-Fig-2c
  *** xIrak1(104-712)[Ab] copptby[WB] Tak1[Ab] is undetectable
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** unaffected by: IL1b (10 min) [stim]
    *** source: 12242293-Fig-4c
  *** xIrak1(del(104-198))[Ab] copptby[WB] IL1R1[Ab] is detectable
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** unaffected by: IL1b (10 min) [stim]
    *** source: 12242293-Fig-2c
  *** xIrak1(del(199-1522))[Ab] copptby[WB] IL1R1[Ab] is increased irt IL1b (10 min)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** source: 12242293-Fig-2c


=================================
coppt by other proteins in IL1R1C
=================================

Mekk3--

  *** Irak1[Ab] copptby[WB] Mekk3[Ab] is increased irt IL1 (times)
    *** cells: HEK293 in BMS
    *** times: 0 5+ 15+ 30+ min
    *** source: 17197697-Fig-4f

Tak1--

  *** Irak1[Ab] copptby[WB] Tak1[Ab] is increased irt IL1b (10 min)
    *** cells: HEK293 in BMS
    *** comment: coppt Irak1 is upshifted
    *** source: 12242293-Fig-4c
  *** Irak1[Ab] copptby[WB] Tak1[Ab] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 1 5+++ 10+ 30+ 60+ 120 240 min
    *** comment: coppt Irak1 upshift increases with time
    *** comment: no total Irak1 control performed
    *** source: 12242293-Fig-4a
  *** xIrak1(del(199-1522))[Ab] copptby[WB] Tak1[Ab] is increased irt IL1b (10 min)
    *** cells: HEK293I1A<Irak1~null> in BMS
    *** source: 12242293-Fig-4c
  *** Irak1[Ab] copptby[WB] Tak1[Ab] is increased irt IL1 (times)
    *** cells: HEK293 in BMS
    *** times: 0 5+ 15++ 30++ min
    *** source: 17197697-Fig-4a

Tab2--

  *** Irak1[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 2+ 5+ 10+ 30+ 40+ 50+ 60+ min
    *** source: 18180283-Fig-5a
  *** Irak1[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 1+ 2++ 5+++ 10+ 20 min
    *** source: 11259596-Fig-5a
  *** Irak1[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0 1 2++ 5+++ 10++ 30++ 60+ 120 240 min
    *** source: 12242293-Fig-4a
  *** Irak1[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 1+ 2++ 5+++ 10+ 20 min
    *** comment: Irak1 upshift increases with time
    *** comment: unmodified[MW] Irak1[Ab] is not coppt
    *** source: 11259596-Fig-5a
  *** Irak1[Ab] copptby[WB] Tab2[Ab] is increased irt IL1b (times)
    *** cells: HEK293T<xIL1R1> in BMS
    *** times: 0 2+++ 5++ 10+ min
    *** comment: coppt Irak1 is ubiq[WBMS]
    *** comment: Irak1 ubiq but not coppt is partially inhibited by addition of rUsp5 to the lysates
    *** source: 19675569-Fig-3b

Traf6--

  *** Irak1[Ab] copptby[WB] Traf6[Ab] is increased irt IL1 (times)
    *** cells: HEK293 in BMS
    *** times: 0 5+ 15++ 30++ min
    *** source: 17197697-Fig-4e


xxxx leave out fractionation evidence until someone confirms with IHC xxxxx

  *** Irak1[Ab] infraction(cytoplasmic)[WB] is decreased irt IL1b (times)
    *** cells: HEK293 in BMS
    *** times: 0++ 3++ 10++ 30+ 60 min
    *** source: 11518704-Fig-1a

