=================================
ArhGef4 is required for Cdc42-GTP
=================================

  *** Cdc42[Ab] GTP-association[BDPD] is detectable
    *** cells: HCT116 in BMS
    *** partially reqs: ArhGef4 [RNAi]
    *** comment: HCT116 expresses full-length Apc
    *** source: 17704816-Fig-8a
  *** Cdc42[Ab] GTP-association[BDPD] is detectable
    *** cells: SW48 in BMS
    *** partially reqs: ArhGef4 [RNAi]
    *** comment: SW48 expresses full-length Apc
    *** source: 17704816-Fig-8a
  *** Cdc42[Ab] GTP-association[BDPD] is detectable
    *** cells: SW480 in BMS
    *** does not req: ArhGef4 [RNAi]
    *** comment: SW480 expresses truncated Apc (1-1338)
    *** source: 17704816-Fig-8a


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rArhGef4 Cdc42 activity is inhibited by SH3 domain
==================================================

  *** rCdc42 GTP-association[Mant-GTP] is increased by rArhGef4(170-632/W203A)
    *** cells: none
    *** partially inhibited by: rArhGef4(170-632/R249A) [substitution]
    *** partially inhibited by: rArhGef4(170-632/R253A) [substitution]
    *** inhibited by: rArhGef4(170-632/R238A) [substitution]
    *** inhibited by: rArhGef4(170-632/R210A) [substitution]
    *** inhibited by: rArhGef4(170-632/R204A) [substitution]
    *** inhibited by: rArhGef4(WT) [substitution]
    *** source: 17704816-Fig-7a
  *** rCdc42 GTP-association[Mant-GTP] is increased by rArhGef4(271-632)"DH/PH"
    *** cells: none
    *** inhibited by: rArhGef4(194-632) [substitution]
    *** inhibited by: rArhGef4(183-632) [substitution]
    *** inhibited by: rArhGef4(170-632) [substitution]
    *** inhibited by: rArhGef4(160-632) [substitution]
    *** inhibited by: rArhGef4(144-632) [substitution]
    *** source: 17704816-Fig-3b

  *** rCdc42 GDP-dissociation[3H-GDP] is unchanged by rArhGef4
    *** cells: none
    *** unaffected by: rApc(mnr)"Arm-domain" [addition]
    *** source: 10947987-Fig-3c

===========================================
rArhGef4 DH domain alone has Cdc42 activity
===========================================

  *** rCdc42 GDP-dissociation[Mant-GDP] is increased by rArhGef4(233-448)"DH"
    *** cells: none
    *** inhibited by: rArhGef4(93-599)"ABR/PH/DH" [substitution]
    *** unaffected by: rArhGef4(93-488)"ABR/DH" [substitution]
    *** source: 17214551-Fig-3b
  *** rCdc42 GDP-dissociation[Mant-GDP] is increased by rArhGef4(233-448)"DH"
    *** cells: none
    *** source: 17214551-Fig-2d

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rArhGef4 PH/DH domain Cdc42 activity requires L421
==================================================

  *** rCdc42 GTP-association[Mant-GTP] is increased by rArhGef4(271-632)"DH/PH"
    *** cells: none
    *** inhibited by: rArhGef4(271-632/L421I)"DH/PH" [substitution]
    *** source: 17704816-Fig-1b

  *** rCdc42 GTP-association[Mant-GTP] is increased by rArhGef4(271-632)"DH/PH"
    *** cells: none
    *** source: 17704816-Fig-1b
  *** rCdc42 GTP-association[Mant-GTP] is increased by rArhGef4(271-632)"DH/PH"
    *** cells: none
    *** source: 17704816-Fig-S1

