===========================
Semi-Direct Phosphorylation
===========================

reqs Flna(S2152)

  *** rFlna[32P] phos[32P-ATP] is increased by xRsk!Chick[tAb]IP
    *** cells: none
    *** IPfrom: HEK293E<xRsk!Chick> in BMLS "treated with serum for tnr"
    *** inhibited by: rFlna(S2152A) [substitution]
    *** source: 15024089-Fig-1b
  *** rFlna[32P] phos[32P-ATP] is increased by xRsk!Chick[tAb]IP
    *** cells: none
    *** IPfrom: HEK293E<xRsk!Chick> in BMLS "treated with Serum for 10 min"
    *** inhibited by: rFlna(S2152A) [substitution]
    *** source: 15024089-Fig-1c
  *** rFlna(1522-1932)[32P] phos[32P-ATP] is unchanged by Rsks[Ab]IP
    *** cells: none
    *** IPfrom: HEK293E in BMLS "treated with serum for 10 min"
    *** source: 15024089-Fig-1a 
  *** rFlna(1522-2283)[32P] phos[32P-ATP] is increased by Rsks[Ab]IP
    *** cells: none
    *** IPfrom: HEK293E in BMLS "treated with serum for 10 min"
    *** source: 15024089-Fig-1a 
  *** rFlna(1933-2346)[32P] phos[32P-ATP] is increased by Rsks[Ab]IP
    *** cells: none
    *** IPfrom: HEK293E in BMLS "treated with serum for 10 min"
    *** source: 15024089-Fig-1a 
  *** rFlna(2489-2647)[32P] phos[32P-ATP] is unchanged by Rsks[Ab]IP
    *** cells: none
    *** IPfrom: HEK293E in BMLS "treated with serum for 10 min"
    *** source: 15024089-Fig-1a 

reqs Rsks kinase-activity

  *** rFlna[32P] phos[32P-ATP] is increased by xRsk!Chick[tAb]IP
    *** cells: none
    *** IPfrom: HEK293E<xRsk!Chick> in BMLS "treated with serum for tnr"
    *** inhibited by: xRsk!Chick(K112R) [substitution]
    *** unaffected by: xRsk!Chick(K464R) [substitution]
    *** unaffected by: xRsk!Chick(K112R/L464R) [substitution]
    *** source: 15024089-Fig-1b

Effect of chemicals

  *** rFlna[32P] phos[32P-ATP] is increased by xRsk!Chick[tAb]IP
    *** cells: none
    *** IPfrom: HEK293E<xRsk!Chick> in BMLS "treated with Serum for 10 min"
    *** inhibited by: Wortmannin [pretreatment] no-bkg-control
    *** source: 15024089-Fig-1c
  *** rFlna[32P] phos[32P-ATP] is increased by xRsk!Chick[tAb]IP
    *** cells: none
    *** IPfrom: HEK293E<xRsk!Chick> in BMLS "treated with Serum for 10 min"
    *** source: 15024089-Fig-2b

No extras

  *** rFlnb[32P] phos[32P-ATP] is increased by xRsk!Chick[tAb]IP
    *** cells: none
    *** IPfrom: HEK293E<xRsk!Chick> in BMLS
    *** source: 15024089-Fig-2b

