requires Traf6--

  *** Tak1[Ab]IP ubiq[UbAb] is increased irt Tgfb1 (times)
    *** cells: mEFs in BMS
    *** times: 0 2+ 5++ 15++ min
    *** reqs: Traf6 [KO]
    *** source: 18758450-Fig-5a
  *** Tak1[Ab]IP ubiq[UbAb] is increased irt Tgfb1 (5 min)
    *** cells: mEFs<xTraf6> in BMS
    *** inhibited by: xTraf6(C70A) [substitution]
    *** source: 18758450-Fig-5b
  *** xTak1[tAb]IP ubiq[tUbAb] is increased irt Tgfb1 (times)
    *** cells: PC3U<xTak1><xUbiq> in BMS
    *** times: 0+ 2++ 5 15+ 30++ min
    *** reqs: Traf6 [RNAi]
    *** source: 18758450-Fig-S6d
  *** xTak1[tAb]IP ubiq[UbAb] is increased irt Tgfb1 (5 min)
    *** cells: HEK293T<xTak1><xTraf6> in BMS
    *** reqs: xTraf6 [omission]
    *** inhibited by: xTraf6(mnr)"DN" [substitution]
    *** source: 18758450-Fig-4a

K63 linked ubiq

  *** xTak1[tAb]IP ubiq[tUbAb] is increased irt Tgfb1 (5 min)
    *** cells: HEK293T<xTak1><xUbiq> in BMS
    *** enhanced by: xUbiq(K63only) [substitution]
    *** inhibited by: xUbiq(K48only) [substitution]
    *** source: 18758450-Fig-1c
  *** xTak1[tAb]IP ubiq[tUbAb] is increased irt Tgfb1 (times)
    *** cells: HEK293T<xTak1><xUbiq> in BMS
    *** times: 0+ 2++ 5+ min
    *** inhibited by: xUbiq(K63R) [substitution]
    *** unaffected by: xUbiq(K63R) [substitution]
    *** source: 18758450-Fig-1b
  *** xTak1[tAb]IP ubiq[tUbAb] is increased irt Tgfb1 (5 min)
    *** cells: HEK293T<xTak1><xUbiq> in BMS
    *** inhibited by: xUbiq(K63R) [substitution]
    *** unaffected by: xUbiq(K48R) [substitution]
    *** source: 18758450-Fig-S2a

requires ubiq site K34R

  *** xTak1[tAb]IP ubiq[tUbAb] is increased irt Tgfb1 (times)
    *** cells: HEK293T<xTak1><xUbiq> in BMS
    *** times: 0 2 5+ 15++ min
    *** inhibited by: xTak1(K34R) [substitution]
    *** source: 18758450-Fig-5f
  *** xTak1[tAb]IP ubiq[UbAb] is increased irt Tgfb1 (times)
    *** cells: HELA<xTak1> in BMS
    *** times: 0+ 5++ 10+ min
    *** inhibited by: xTak1(K158R) [substitution]
    *** unaffected by: xTak1(K34R) [substitution]
    *** source: 20837137-Fig-3


no extras

  *** xTak1[tAb]IP ubiq[tUbAb] is increased irt Tgfb1 (times)
    *** cells: HEK293T<xTak1><xUbiq> in BMS
    *** times: 0 2+ 5+ 15+ min
    *** source: 18758450-Fig-1a
  *** xTak1[Ab]IP ubiq[UbAb] is increased irt Tgfb1 (5 min)
    *** cells: HEK293T<xTak1><xUbiq> in BMS
    *** source: 18758450-Fig-1d
  *** xTak1[tAb]IP ubiq[tUbAb] is increased irt Tgfb1 (times)
    *** cells: HEK293T<xTak1><xUbiq> in BMS
    *** times: 0+ 2+ 5++ 15+ min
    *** source: 18758450-Fig-S1g


