===================
Relocation to Gp130
===================

  *** Shp2[Ab] copptby[WB] Gp130[Ab] is increased irt IL6 (30 min)
    *** cells: HEPG2<xLmo4> in BMS
    *** source: 15677447-Fig-4
  *** Shp2[Ab] copptby[WB] Gp130[Ab] is increased irt IL6 (times)
    *** cells: HEPG2 in BMS
    *** times: 0 5+ 10 20 40 min
    *** source: 9794795-Fig-1c

Contradiction

  *** Shp2[Ab] copptby[WB] Gp130[Ab] is unchanged irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** source: 9632795-Fig-1c

===================================================
Coprecipitation with other members of Gp130 complex
===================================================

Gab1--

  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt IL6 (2 min)
    *** cells: MM1S in BMLS
    *** source: 15010462-Fig-2b
  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** source: 9632795-Fig-1c
  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt IL6 (times)
    *** cells: HEPG2 in BMS
    *** times: 0 5+ 10+ 30 60 min
    *** source: 9632795-Fig-1a
  *** Shp2[Ab] copptby[WB] Gab1[Ab] is increased irt IL6 (times)
    *** cells: MM1S in BMLS
    *** times: 0 1+ 5++ min
    *** inhibited by: xHck(K269E) [substitution]
    *** source: 15010462-Fig-5c
  *** Shp2[Ab] copptby[WB] xGab1[tAb] is increased irt IL6 (tnr)
    *** cells: HEK293<xHck> in BMLS
    *** inhibited by: xHck(K269E) [substitution]
    *** source: 15010462-Fig-5e
  *** Shp2[Ab] copptby[WB] xGab2[tAb] is increased irt IL6 (tnr)
    *** cells: HEK293<xHck> in BMLS
    *** inhibited by: xHck(K269E) [substitution]
    *** source: 15010462-Fig-5f

Gab2--

  *** Shp2[Ab] copptby[WB] Gab2[Ab] is increased irt IL6 (2 min)
    *** cells: MM1S in BMLS
    *** source: 15010462-Fig-2b
  *** Shp2[Ab] copptby[WB] Gab2[Ab] is increased irt IL6 (times)
    *** cells: MM1S in BMLS
    *** times: 0 1+ 5++ min
    *** inhibited by: xHck(K269E) [substitution]
    *** source: 15010462-Fig-5d

Grb2--

  *** Shp2[Ab] copptby[WB] Grb2[Ab] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** source: 9632795-Fig-1c
  *** Shp2[Ab] copptby[WB] Grb2[Ab] is increased irt IL6 (times)
    *** cells: MM1S in BMLS
    *** times: 0 1+ 5++ min
    *** inhibited by: PP2 [pretreatment]
    *** source: 15010462-Fig-4b
  *** Shp2[Ab] copptby[WB] Grb2[Ab] is increased irt IL6 (times)
    *** cells: MM1S<xHck> in BMLS
    *** times: 0 1+ 5++ min
    *** inhibited by: xHck(K269E) [substitution]
    *** source: 15010462-Fig-6b

Pik3rs--

  *** Shp2[Ab] copptby[WB] Pik3rs[Ab] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** source: 9632795-Fig-1c
  *** Shp2[Ab]IP IVLKA(PtdIns)[32P-ATP] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** source: 9632795-Fig-1d
  *** Shp2[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: 2FTGH in BMS
    *** source: 9794795-Fig-3a

===========================
Phosphorylation on Tyrosine
===========================

reqs Gp130--

  *** Shp2[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: BAF3<xGp130> in BMS
    *** reqs: xGp130 [omission]
    *** inhibited by: xGp130(Y759F) [substitution]
    *** source: 9794795-Fig-2a

Hck--  ???

  *** Shp2[Ab]IP Yphos[pYAb] is increased irt IL6 (times)
    *** cells: MM1S<xHck> in BMLS
    *** times: 0+ 1++ 5++ min
    *** bkg inhibited by: xHck(K269E) [substitution]
    *** source: 15010462-Fig-6a

inhibited by Socs3--

  *** Shp2[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: COS7 in BMS
    *** inhibited by: xSocs3 [addition] 
    *** source: 10777583-Fig-3
  *** Shp2[phosAb] phos(sitenr)[phosAb] is increased irt IL6 (times)
    *** cells: mBMMs in BMS
    *** times: 0 25++ 100+ 400 min
    *** comment: repressed by Socs3 [KO] at 400 min only
    *** source: 12754506-Fig-4a

reqs Jak1--

  *** Shp2[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: U4A<Jak1~null><xJak1> in BMS
    *** reqs: xJak1 [omission]
    *** source: 9794795-Fig-3d

does not req Jak2--

  *** Shp2[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: GAMMA2A<Jak2~null><xJak2> in BMS
    *** does not req: xJak2 [omission]
    *** source: 9794795-Fig-3b

does not req Tyk2--

  *** Shp2[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: U1A<Tyk2~null><xTyk2> in BMS
    *** does not req: xTyk2 [omission]
    *** source: 9794795-Fig-3c

Chemicals

  *** Shp2[Ab]IP Yphos[pYAb] is increased irt IL6 (times)
    *** cells: MM1S in BMLS
    *** times: 0 1+ 5+ min
    *** inhibited by: PP2 [pretreatment]
    *** source: 15010462-Fig-4a

No extras

  *** Shp2[Ab]IP Yphos[pYAb] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** source: 9632795-Fig-1c
  *** Shp2[Ab]IP Yphos[pYAb] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** source: 9794795-Fig-1a
  *** Shp2[Ab]IP Yphos[pYAb] is increased irt IL6 (times)
    *** cells: HEPG2 in BMS
    *** times: 0 5+ 10++ 20+ 40 min
    *** source: 9794795-Fig-1b



