==============================
Location of endogenous protein
==============================

  *** Nfkb1[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: HUH7<xRela> in BMS "treated with LMB"
    *** unaffected by: xRela(K301A/R302A/K303A)"NLS-mut" [substitution]
    *** source: 15677444-Fig-6c


===================================
Location of expressed protein {p50}
===================================

  *** xNfkb1(1-433)[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: HEK293<xNfkb1(1-433)> in BMS
    *** inhibited by: xIkba [addition]
    *** source: 9566883-Fig-3a
  *** xNfkb1(1-433)[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: HEK293<xNfkb1(1-433)> in BMS
    *** inhibited by: xNfkb1((1-433)/del(361-366))"delNLS" [substitution]
    *** source: 9566883-Fig-3a
  *** xNfkb1(1-433)[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: HEK293<xNfkb1(1-433)> in BMS
    *** inhibited by: xRela(189-337/del(301-304))"dCdNLS" [addition]
    *** source: 9566883-Fig-3a
  *** xNfkb1(1-433)[tAb] locatedin(nucleus)[IHC] is detectable
    *** cells: HUH7<xNfkb1(1-433)> in BMS
    *** inhibited by: xNfkb1((1-433)/K362A/R363A)"NLS-mut" [substitution]
    *** source: 15677444-Fig-6c
  *** xNfkb1(1-433)[tAb] locatedin(nucleus)[IHC] is detectable
    *** cells: HUH7<xNfkb1(1-433)> in BMS
    *** unaffected by: Tnf [addition]
    *** source: 15677444-Fig-6c
  *** xNfkb1(1-433)[tAb] locatedin(nucleus)[IHC] is detectable
    *** cells: HUH7<xRela> in BMS "treated with LMB"
    *** unaffected by: Tnf [addition]
    *** source: 15677444-Fig-6c
  *** xNfkb1(246-399)"delN"[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: HEK293<xNfkb1(246-399)"delN"> in BMS
    *** inhibited by: xIkba [addition]
    *** source: 9566883-Fig-2a
  *** xNfkb1(1-433)[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: COS7<xNfkb1(1-433)> in BMS
    *** inhibited by: xNfkb1((1-433)/K363N/R364S) [substitution]
    *** source: 1547506-Fig-1
  *** xNfkb1(1-433)[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: VERO<xNfkb1(1-433)> in BMS
    *** inhibited by: xNfkb1((1-433)/K363N/R364S) [substitution]
    *** source: 1547506-Fig-1

=====================================
Location of expressed protein {p105}
=====================================

  *** xNfkb1{p105}[Ab] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS7<xNfkb1{p105}> in BMS
    *** source: 1547506-Fig-1a
  *** xNfkb1{p105}[Ab] locatedin(cytoplasm)[IHC] is detectable
    *** cells: VERO<xNfkb1{p105}> in BMS
    *** source: 1547506-Fig-1a











================================
Associations with other Proteins
================================

Flip--

  *** xNfkb1(1-433)[tAb] copptby[WB] xFlip[tAb] is detectable
    *** cells: HEK293 in BMS
    *** comment: coppt Nfkb1 is 105 kD not 50 kD
    *** source: 13679070-Fig-1a

Ikba--

  *** xNfkb1(246-399)"delN"[Ab] copptby[WB] xIkba[Ab] is detectable
    *** cells: HEK293<xNfkb1(246-399)"delN"><xIkba> in BMS
    *** source: 9566883-Fig-2b
  *** xNfkb1(1-433)[35S] copptby[WB] xIkba[Ab] is detectable
    *** cells: HEK293<xNfkb1(1-433)><xIkba> in BMS
    *** inhibited by: xNfkb1(del(361-366))"delNLS" [substitution]
    *** source: 9566883-Fig-3b

Kpna1--

  *** rNfkb1[35S] boundby[WB] rKpna1[35S] is detectable
    *** cells: none
    *** inhibited by: rNfkb1(K362A/R363A)"NLS-mut" [substitution]
    *** source: 15677444-Fig-6b
  *** rNfkb1[35S] boundby[WB] rKpna1[35S] is detectable
    *** cells: none
    *** source: 15677444-Fig-5a

Kpna3--

  *** rNfkb1[35S] boundby[WB] rKpna3[35S] is detectable
    *** cells: none
    *** inhibited by: rKpna3(mnr)"ARM3" [substitution]
    *** unaffected by: rKpna3(mnr)"ARM7+8" [substitution]
    *** inhibited by: rKpna3(mnr)"ARM3+8" [substitution]
    *** source: 15677444-Fig-7c
  *** rNfkb1[35S] boundby[WB] rKpna3[35S] is detectable
    *** cells: none
    *** inhibited by: rNfkb1(K362A/R363A)"NLS-mut" [substitution]
    *** source: 15677444-Fig-6a
  *** rNfkb1[35S] boundby[WB] rKpna3[35S] is detectable
    *** cells: none
    *** source: 15677444-Fig-5a
  *** rNfkb1[35S] boundby[WB] rKpna3[35S] is detectable
    *** cells: none
    *** unaffected by: rIkba [addition]
    *** partially inhibited by: rIkba + rRela [addition]
    *** source: 15677444-Fig-5b

Kpna5--

  *** rNfkb1[35S] boundby[WB] rKpna5[35S] is undetectable
    *** cells: none
    *** source: 15677444-Fig-5a

Kpna6--

  *** rNfkb1[35S] boundby[WB] rKpna6[35S] is undetectable
    *** cells: none
    *** source: 15677444-Fig-5a
  *** rNfkb1[35S] boundby[WB] rKpnb1[35S] is undetectable
    *** cells: none
    *** source: 15677444-Fig-5a

Mdm2--

  *** rNfkb1[35S] boundby[WB] rMdm2[GST] is undetectable
    *** cells: none
    *** source: 11046142-Fig-5a

Pin1--

  *** Nfkb1[Ab] snaggedby[WB] rPin1[GST] is undetectable
    *** cells: HELA in BMS
    *** source: 14690596-Fig-3a

Pkca?--

  *** rNfkb1[35S] boundby[WB] rPkac?[GST] is undetectable
    *** cells: none
    *** source: 9150141-Fig-3c

Ripk1--

  *** xNfkb1(1-433)[Ab] copptby[WB] Ripk1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 16291755-Fig-2c

======================
Associatons with Genes
======================


CcL2-gene--

  *** Nfkb1[Ab] boundto(CcL2-gene)[ChIP] is detectable
    *** cells: mEFs in BMS
    *** reqs: Jun [KO]
    *** source: 18443042-Fig-8b



========
Cleavage
========


  *** Nfkb1[Ab] cleavage[WB] is detectable
    *** cells: mLNBCs in BMS
    *** does not req: Ikk1 [KO]
    *** source: 11520989-Fig-2d
  *** Nfkb1[Ab] cleavage[WB] is detectable
    *** cells: mSpBCs in BMS
    *** does not req: Ikk1 [KO]
    *** source: 11520989-Fig-2d
  *** xNfkb1(1-433)[tAb] cleavage[WB] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xFlip [addition]
    *** source: 13679070-Fig-4a


