==========
Mlst8-Sin1
==========

Mlst8-Sin1 does not req Protor1

  *** Mlst8[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HELA in BMS
    *** does not req: Protor1 [RNAi]
    *** source: 17461779-Fig-4c

============
Mlst8-Rictor
============

Mlst8-Rictor does not req Protor1

  *** Mlst8[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HELA in BMS
    *** does not req: Protor1 [RNAi]
    *** source: 17461779-Fig-4c



===========
Mtor-Rictor
===========

Mtor-Rictor reqs Mlst8

  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: mEFs in BMS
    *** reqs: Mlst8 [KO]
    *** source: 18925875-Fig-3d

Mtor-Rictor reqs Sin1

  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: mEFs in BMLS
    *** reqs: Sin1 [KO]
    *** source: 16962653-Fig-5d
  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: mEFs in BMLS
    *** reqs: Sin1 [KO]
    *** source: 16962653-Fig-5c,6c
  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** reqs: Sin1 [RNAi]
    *** source: 17043309-Fig-3c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** reqs: Sin1 [RNAi]
    *** source: 17043309-Fig-3e
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** reqs: Sin1 [RNAi]
    *** source: 16919458-Fig-3a
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** reqs: Sin1 [RNAi]
    *** source: 16919458-Fig-3a
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** reqs: Sin1 [RNAi]
    *** source: 17277771-Fig-S1d

Mtor-Rictor requires Pld1-- Pld2-- or PA

  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: 786O in BMS
    *** inhibited by: xPld1(K898R)"CIA" [addition]
    *** inhibited by: xPld2(K758R)"CIA" [addition]
    *** source: 19114562-Fig-3c

Mtor-Rictor does not req Protor1

  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HELA in BMS
    *** does not req: Protor1 [RNAi]
    *** source: 17461779-Fig-4c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** does not req: Protor1 [RNAi]
    *** source: 17461779-Fig-4c

Mtor-Rictor is inhibited by Raptor

  *** xRictor[tAb] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T<xRaptor(mnr)"Mtor-binding-mut"> in BMS
    *** inhibited by: xRaptor [substitution]
    *** source: 15268862-Fig-2c

Mtor-Rictor does not req Sgk1

  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: WM35<xSgk1> in BMS
    *** unaffected by: xSgk1(S422A) [substitution]
    *** unaffected by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5b

===========
Mtor - Sin1
===========

Mtor-Sin1 does not req Protor1

  *** Mtor[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HELA in BMS
    *** does not req: Protor1 [RNAi]
    *** source: 17461779-Fig-4c
  *** xMtor[Ab] copptby[WB] xSin1[Ab] is detectable
    *** cells: HEK293<xMtor><xRictor><xSin1><xProtor1><xMlst8> in BMS
    *** does not req: xProtor1 [omission]
    *** source: 17461779-Fig-4a


Mtor-Sin1 reqs Rictor

  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** reqs: Rictor [RNAi]
    *** source: 16919458-Fig-3a
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** reqs: Rictor [RNAi]
    *** source: 16919458-Fig-3a
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** reqs: Rictor [RNAi]
    *** comment: Sin1 prot-exp is decreased itao Rictor [RNAi]
    *** source: 17277771-Fig-S1d
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is undetectable
    *** cells: mEFs<Rictor~null> in BMS
    *** source: 18925875-Fig-4a
  *** xMtor[Ab] copptby[WB] xSin1[Ab] is detectable
    *** cells: HEK293<xMtor><xRictor><xSin1><xProtor1><xMlst8> in BMS
    *** reqs: xRictor [omission]
    *** source: 17461779-Fig-4a

Mtor-Sin1 does not req Sgk1

  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: WM35<xSgk1> in BMS
    *** unaffected by: xSgk1(S422A) [substitution]
    *** unaffected by: xSgk1(T256A) [substitution]
    *** unaffected by: xSgk1(F385A) [substitution]
    *** source: 18570873-Fig-5b

Mtor-Sin1 is inhibited by Raptor

  *** xMtor[tAb] copptby[WB] xSin1[Ab] is undetectable
    *** cells: HEK293<xRaptor> in BMS
    *** source: 17043309-Fig-3d
  *** xMtor[tAb] copptby[WB] xSin1[Ab] is undetectable
    *** cells: HEK293<xRaptor> in BMS
    *** source: 17043309-Fig-4b

Mtor-Sin1 is unaffected by Rapamycin

  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: Rapamycin [addition]
    *** source: 16919458-Fig-4b
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** unaffected by: Rapamycin [addition]
    *** source: 16919458-Fig-4b

===========
Rictor-Sin1
===========

Rictor-Sin1 does not req Mtor

  *** xRictor[Ab] copptby[WB] xSin1[Ab] is detectable
    *** cells: HEK293<xMtor><xRictor><xSin1><xProtor1><xMlst8> in BMS
    *** does not req: xMtor [omission]
    *** source: 17461779-Fig-4a

Rictor-Sin1 does not req Mlst8

  *** Sin1[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: mEFs in BMS
    *** does not req: Mlst8 [KO]
    *** source: 18925875-Fig-3d

Rictor-Sin1 does not req Protor1

  *** Rictor[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HELA in BMS
    *** does not req: Protor1 [RNAi]
    *** source: 17461779-Fig-4c
  *** Sin1[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HELA in BMS
    *** does not req: Protor1 [RNAi]
    *** source: 17461779-Fig-4c

================
Lysis Conditions
================

  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** comment: coppt reqs CHAPS or NP40 detergent not Triton
    *** source: 17043309-Fig-3a
  *** xSin1[tAb] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** comment: Coppt works in both Chaps-LB and NP40-LB
    *** source: 17043309-Fig-3c
  *** Mtor[Ab] copptby[WB] xRictor[tAb] is detectable
    *** cells: HEK293T in BMS
    *** comment: Coppt works in Chaps-LB but not TritonX-LB
    *** source: 15268862-Fig-1d
  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: Ppt works in Chaps-LB but not TritonX-LB
    *** source: 15268862-Fig-2b
  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** comment: coppt reqs CHAPS detergent not Triton or NP40
    *** source: 17043309-Fig-3a
  *** Mtor[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** comment: coppt reqs CHAPS detergent not Triton or NP40
    *** source: 17043309-Fig-3a
  *** Rictor[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** comment: Coppt works in Chaps-LB, NP40-LB, Triton-LB
    *** source: 17043309-Fig-3a
  *** xRictor[tAb] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: Coppt works in Chaps-LB but not Triton-LB
    *** source: 15268862-Fig-1d
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: Ppt works in Chaps-LB but not TritonX-LB
    *** source: 15268862-Fig-2b
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** comment: Coppt works in Chaps-LB, NP40-LB, but not Triton-LB
    *** source: 17043309-Fig-3a

=========
Chemicals
=========

  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: Rapamycin [addition]
    *** source: 16919458-Fig-4b
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** unaffected by: Rapamycin [addition]
    *** source: 16919458-Fig-4b
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** comment: unaffected by addition of Rapamycin to lysates for 1 or 24 hr
    *** source: 16603397-Fig-S1

=========
no extras
=========

  *** Mlst8[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-1b
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: A549 in BMS
    *** source: 16919458-Fig-1c
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 16919458-Fig-1b
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 16919458-Fig-1c
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 16919458-Fig-1b,1c
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: JURKAT<Pten~null> in BMS
    *** source: 16919458-Fig-1c
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: LNCAP in BMS
    *** source: 16919458-Fig-1c
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: MCF7 in BMS
    *** source: 16919458-Fig-1c
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: PC3 in BMS
    *** source: 16919458-Fig-1c
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: SHSY5Y in BMS
    *** source: 16919458-Fig-1c
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 17043309-Fig-S5
  *** Sin1[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17043309-Fig-3a
  *** Sin1[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 17043309-Fig-S5
  *** Sin1[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17043309-Fig-3c
  *** Sin1[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 16919458-Fig-1b
  *** Sin1[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 16919458-Fig-1b
  *** Sin1[MS] copptby[WB] Rictor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 16962653-Fig-1a
  *** Mtor[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 16962653-Fig-1b
  *** Mtor[Ab] copptby[WB] xSin1[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 16962653-Fig-S1a
  *** Mtor[MS] copptby[WB] Sin1[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 16962653-Fig-1a
  *** Mtor[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 17043309-Fig-S5
  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 15268862-Fig-S1
  *** Mtor[MS] copptby[WB] Rictor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 16962653-Fig-1a
  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 17043309-Fig-S5
  *** Ilk[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: MDAMB231 in BMS
    *** source: 18339839-Fig-1b
  *** Ilk[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: PC3 in BMS
    *** source: 18339839-Fig-1b
  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: MDAMB231 in BMS
    *** source: 18339839-Fig-1b
  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: PC3 in BMS
    *** source: 18339839-Fig-1b
  *** xMtor[tAb] copptby[WB] xRictor[tAb] is detectable
    *** cells: HEK293T<xMtor><xRictor><xPras40> in BMS
    *** source: 17510057-Fig-1c
  *** Rictor[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 16962653-Fig-1b
  *** Rictor[Ab] copptby[WB] xSin1[tAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 16962653-Fig-S1a
  *** Rictor[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 17043309-Fig-S5
  *** xRictor[tAb] copptby[WB] xSin1[Ab] is detectable
    *** cells: HEK293<xMtor> in BMS
    *** source: 17043309-Fig-3d
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: DU145 in BMS
    *** source: 15268862-Fig-2a
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 15268862-Fig-1c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 15268862-Fig-2a
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 15268862-Fig-2a
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: A549 in BMS
    *** source: 16919458-Fig-1c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 16919458-Fig-1c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 16919458-Fig-1c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: JURKAT<Pten~null> in BMS
    *** source: 16919458-Fig-1c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: LNCAP in BMS
    *** source: 16919458-Fig-1c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: MCF7 in BMS
    *** source: 16919458-Fig-1c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: PC3 in BMS
    *** source: 16919458-Fig-1c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: SHSY5Y in BMS
    *** source: 16919458-Fig-1c
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 17043309-Fig-S5
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: PC3 in BMS
    *** source: 18339839-Fig-1b
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: MDAMB231 in BMS
    *** source: 18339839-Fig-1b
  *** Mlst8[Ab] copptby[WB] xRictor[tAb] is detectable
    *** cells: HEK293T<xRictor> in BMS
    *** source: 17510057-Fig-1b
  *** Mtor[Ab] copptby[WB] xRictor[tAb] is detectable
    *** cells: HEK293T<xRictor> in BMS
    *** source: 17510057-Fig-1b
  *** xMtor[tAb] copptby[WB] xSin1[Ab] is detectable
    *** cells: HEK293<xRictor> in BMS
    *** source: 17043309-Fig-4b
  *** xMtor[tAb] copptby[WB] xSin1[Ab] is detectable
    *** cells: HEK293<xRictor> in BMS
    *** source: 17043309-Fig-3d
  *** Mtor[Ab] copptby[WB] xSin1[tAb] is detectable
    *** cells: HEK293T<xSin1> in BMS
    *** source: 17277771-Fig-S1c
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-S1a
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-S1b
  *** Sin1[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-S1b
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-1b
  *** Mlst8[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 18925875-Fig-3a
  *** Mlst8[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: mEFs in BMS
    *** source: 18925875-Fig-3d
  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 18925875-Fig-3a
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17510057-Fig-1b

  *** Sin1[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 18925875-Fig-3a
  *** Mtor[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17461779-Fig-3a
  *** Mtor[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17461779-Fig-3a
  *** Rictor[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17461779-Fig-3a
  *** Rictor[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17461779-Fig-3a
  *** Sin1[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17461779-Fig-3a
  *** Sin1[Ab] copptby[WB] Protor1[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 17461779-Fig-4c
  *** Mlst8[Ab] copptby[WB] Sin1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17461779-Fig-3a
  *** Mlst8[Ab] copptby[WB] Rictor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17461779-Fig-3a
