=====================
Raf1 Kinase Activity
=====================

  *** Raf1[Ab]IP IVKA(auto)[32P-ATP] is increased irt IL2 (times)
    *** cells: hIL2RTCs in BMHIS "IL2-deprived for 12-14 hr"
    *** times: 0 2 5+ 15+ 30+ 360+ 600+ 840+ min
    *** comment: no control for total Raf1 protein
    *** source: 1708096-Fig-4a
  *** Raf1[Ab]IP IVKA(HistH1)[32P-ATP] is increased irt IL2 (times)
    *** cells: hPHAblasts in BMS
    *** times: 0+ 15++ min
    *** source: 1639773-Fig-2
  *** Raf1[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt IL2 (5 min)
    *** cells: CTLL2 in BMS "IL2-deprived"
    *** enhanced by: Wortmannin [pretreatment]
    *** source: 7760801-Fig-9
  *** Raf1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt IL2 (10 min)
    *** cells: CTLL2 in BMS
    *** source: 1996324-Fig-1
  *** Raf1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt IL2 (10 min)
    *** cells: CTLL2 in BMS
    *** source: 1996324-Fig-3a
  *** Raf1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt IL2 (times)
    *** cells: CTLL2 in BMS
    *** times: 0 10+ 15++ 20+++ 25++ 30+ 40+ min
    *** source: 1996324-Fig-2c

==========================
Raf1 Total Phosphorylation
==========================

  *** Raf1[Ab] phos[WBMS] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** inhibited by: PD98059 [pretreatment]
    *** source: 9305919-Fig-5a
  *** Raf1[Ab]IP phos[32Pi] is increased irt IL2 (10 min)
    *** cells: CTLL2 in BMS
    *** source: 1996324-Fig-1
  *** Raf1[Ab]IP phos[32Pi] is increased irt IL2 (times)
    *** cells: CTLL2 in BMS
    *** times: 0 2+ 20++ min
    *** comment: increased phos was on pS and pY but not pT by PAA
    *** source: 1996324-Fig-6
  *** Raf1[Ab]IP phos[32Pi] is increased irt IL2 (times)
    *** cells: CTLL2 in BMS
    *** times: 0 15++ 30++ 60+ 120+ min
    *** source: 1996324-Fig-2b

=============================
Raf1 Tyrosine Phosphorylation
=============================

  *** Raf1[Ab]IP Yphos[pYAb] is increased irt IL2 (times)
    *** cells: CTLL2 in BMS
    *** times: 0 2+ 5+ 20++ min
    *** source: 1996324-Fig-4
  *** Raf1[Ab] Yphos[pYAb-ppt] is increased irt IL2 (10 min)
    *** cells: CTLL2 in BMS
    *** source: 1996324-Fig-5

=======================
What is this all about?
=======================

  *** Raf1[Ab] copptby[WB] IL2Rb[Ab] is decreased irt IL2 (times)
    *** cells: hPHAblasts in BMS
    *** times: 0+ 5 15 min
    *** source: 1639773-Fig-1b
  *** Raf1[Ab] copptby[WB] IL2Rb[Ab] is decreased irt IL2 (times)
    *** cells: hPHAblasts in BMS
    *** times: 0+ 15 min
    *** inhibited by: Genistein [pretreatment]
    *** source: 1639773-Fig-3b
