==========
Activation
==========

  *** S6k1[Ab]IP IVKA(Rps6)[32P-ATP] is increased irt Tnf (30 min)
    *** cells: MCF7 in BMLS
    *** source: 17693255-Fig-1b

==================================
Phosphorylation on T412 (CST T389)
==================================

not Akt1--

  *** xS6k1[tAb]IP  phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7 in BMLS
    *** does not req: Akt1 or Akt2 or Akt3 [RNAi]
    *** source: 17693255-Fig-S2a

not Erks--

  *** xS6k1[tAb]IP  phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7 in BMLS
    *** does not req: Erk1 or Erk2 [RNAi]
    *** source: 17693255-Fig-S2b

not Ikba--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7 in BMLS
    *** unaffected by: xIkba(mnr)"DN" [addition]
    *** source: 17693255-Fig-1d

Ikk2--

  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7<xS6k1> in BMLS
    *** reqs: Ikk2 [RNAi]
    *** source: 17693255-Fig-3f
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7<xS6k1> in BMS
    *** reqs: Ikk2 [RNAi]
    *** source: 17693255-Fig-2g

Mtor--

  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7<xS6k1> in BMLS
    *** reqs: Mtor [RNAi]
    *** source: 17693255-Fig-1c

Nemo--

  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7<xS6k1> in BMS
    *** reqs: Nemo [RNAi]
    *** source: 17693255-Fig-2g

not P38s--

  *** xS6k1[tAb]IP  phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7 in BMLS
    *** does not req: P38? [RNAi]
    *** source: 17693255-Fig-S2b

not Tsc1--  inhibition

  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs<Tsc1~null> in BMLS
    *** source: 17693255-Fig-S3a
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs<Tsc1~null><xS6k1> in BMLS
    *** source: 17693255-Fig-5b
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs<Tsc1~null><xS6k1> in BMLS
    *** source: 17693255-Fig-5c
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs<Tsc1~null><xTsc1><xS6k1> in BMLS
    *** inhibited by: xTsc1(S487A/S511A) [substitution]
    *** partially inhibited by: xTsc1(S487A) [substitution]
    *** partially inhibited by: xTsc1(S511A) [substitution]
    *** unaffected by: xTsc1(S487D/S511D) [substitution]
    *** source: 17693255-Fig-5c
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs<Tsc1~null><xTsc1><xS6k1> in BMLS
    *** partially inhibited by: xTsc1(S487A/S511A) [substitution]
    *** unaffected by: xTsc1(S487D/S511D) [substitution]
    *** source: 17693255-Fig-5b

not Tsc2--

  *** xS6k1[tAb]IP phos(T412)[phosAb] is detectable-but unchanged irt Tnf (30 min)
    *** cells: mEFs<Tsc2~null> in BMLS
    *** source: 17693255-Fig-S3a

chemicals

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7 in BMLS
    *** bkg inhibited by: Bay117082 [pretreatment]
    *** bkg inhibited by: Parthenolide [pretreatment]
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** source: 17693255-Fig-2h

no extras

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: HBL100 in BMS
    *** source: 17693255-Fig-2c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7 in BMLS
    *** source: 17693255-Fig-3d
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF7 in BMS
    *** source: 17693255-Fig-3a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: MCF10A in BMS
    *** source: 17693255-Fig-2c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs in BMLS
    *** source: 17693255-Fig-4a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs<Tp53~null> in BMLS
    *** source: 17693255-Fig-4a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: RAW2647 in BMS
    *** source: 17693255-Fig-3a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (times)
    *** cells: MCF7 in BMLS
    *** times: 0 5+ 10++ 20++ 30++ min
    *** source: 17693255-Fig-1a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Tnf (times)
    *** cells: MDAMB453 in BMLS
    *** times: 0 5+ 10++ 20++ 30++ min
    *** source: 17693255-Fig-1a
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs<Tsc2~null><xTsc2> in BMLS
    *** source: 17693255-Fig-S3a
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Tnf (30 min)
    *** cells: mEFs<Tsc1~null><xTsc1> in BMLS
    *** source: 17693255-Fig-S3a
