===================
Recruitment to HgfR
===================

  *** HgfR[Ab] copptby[WB] Gab1[Ab] is increased irt Hgf (times)
    *** cells: HEK293T in BMS
    *** times: 0 10++ 20++ 30++ min
    *** source: 11894096-Fig-4a
  *** Gab1[Ab] copptby[WB] HgfR[Ab] is increased irt Hgf (5 min)
    *** cells: HEK293 in BMLS
    *** unaffected by: xMuc20 [addition]
    *** source: 15314156-Fig-2e
  *** HgfR[Ab] copptby[WB] Gab1[Ab] is increased irt Hgf (5 min)
    *** cells: HEK293 in BMLS
    *** unaffected by: xMuc20 [addition]
    *** source: 15314156-Fig-2e

Coprecipitation with Crk which coppts HgfR irt Hgf

  *** Crk[Ab] copptby[WB] Gab1[Ab] is increased irt Hgf (15 min)
    *** cells: A549 in BMLS
    *** source: 10618718-Fig-3c
  *** CrkI[Ab] copptby[WB] Gab1[Ab] is increased irt Hgf (15 min)
    *** cells: A549 in BMLS
    *** source: 10618718-Fig-3c
  *** xGab1[tAb] snaggedby[WB] rCrk(mnr)"SH2"[GST] is increased irt Hgf (15 min)
    *** cells: MDCK<xGab1> in BMLS
    *** source: 12058075-Fig-2c

Coprecipitation with CrkL requires Crk-binding-site

  *** CrkL[Ab] copptby[WB] xGab1[Ab] is increased irt Hgf (2 min)
    *** cells: HEK293<xGab1> in BMS
    *** inhibited by: xGab1(del(242-410))"Crk-binding-sites" [substitution]
    *** source: 10753869-Fig-3d

Coprecipitation with Pik3rs

  *** xGab1[Ab] copptby[WB] Pik3rs[Ab] is increased irt Hgf (20 min)
    *** cells: MIMCD3<xGab1> in BMS
    *** partially inhibited by: U0126 [pretreatment]
    *** source: 11445578-Fig-3b
  *** Gab1[Ab] snaggedby[WB] rPik3r?(mnr)"CSH2"[GST] is increased irt Hgf (tnr)
    *** cells: MIMCD3 in BMS
    *** partially inhibited by: U0126 [pretreatment]
    *** source: 11445578-Fig-2b
  *** Gab1[Ab] snaggedby[WB] rPik3r?(mnr)"NSH2"[GST] is increased irt Hgf (tnr)
    *** cells: MIMCD3 in BMS
    *** partially inhibited by: U0126 [pretreatment]
    *** source: 11445578-Fig-2b
  *** Gab1[Ab] snaggedby[WB] rPik3r?[GST] is increased irt Hgf (tnr)
    *** cells: MIMCD3 in BMS
    *** partially inhibited by: U0126 [pretreatment]
    *** source: 11445578-Fig-2a
  *** xGab1[tAb] copptby[WB] Pik3rs[Ab] is increased irt Hgf (times)
    *** cells: MDCK<xGab1> in BMLS
    *** times: 0 15+ 30+ 60+ min
    *** unaffected by: xGab1(mnr)"delShp2" [substitution]
    *** source: 11046147-Fig-7b
  *** xGab1[tAb] snaggedby[WB] rPik3r?(mnr)"CSH2"[GST] is increased irt Hgf (15 min)
    *** cells: MDCK<xGab1> in BMLS
    *** source: 12058075-Fig-2c
  *** xGab1[tAb] snaggedby[WB] rPik3r?(mnr)"NSH2"[GST] is increased irt Hgf (15 min)
    *** cells: MDCK<xGab1> in BMLS
    *** source: 12058075-Fig-2c

Association with Grb2--

  *** xGab1[tAb] snaggedby[WB] rGrb2(mnr)"SH2"[GST] is unchanged irt Hgf (15 min)
    *** cells: HEK293<xGab1> in BMLS
    *** source: 12941962-Fig-4c
  *** xGab1[tAb] snaggedby[WB] rGrb2(mnr)"SH3"[GST] is detectable-but unchanged irt Hgf (15 min)
    *** cells: HEK293<xGab1> in BMLS
    *** source: 12941962-Fig-4c

Association with Src--

  *** xGab1[tAb] snaggedby[WB] rSrc(mnr)"SH2"[GST] is increased irt Hgf (15 min)
    *** cells: HEK293<xGab1> in BMLS
    *** source: 12941962-Fig-4c
  *** xGab1[tAb] snaggedby[WB] rSrc(mnr)"SH2"[GST] is increased irt Hgf (15 min)
    *** cells: mEFs<Src~null><Yes1~null><Fyn~null><xGab1> in BMLS
    *** source: 12941962-Fig-4d
  *** xGab1[tAb] snaggedby[WB] rSrc(mnr)"SH3"[GST] is detectable-but unchanged irt Hgf (15 min)
    *** cells: HEK293<xGab1> in BMLS
    *** source: 12941962-Fig-4c

================================
Gab1 Phosphorylation on Tyrosine
================================

does not require binding to Crk-- or CrkL--

  *** xGab1[Ab]IP Yphos[pYAb] is increased irt Hgf (2 min)
    *** cells: HEK293<xGab1> in BMS
    *** unaffected by: xGab1(del(242-410))"Crk-binding-sites" [substitution]
    *** source: 10753869-Fig-3d

does not require binding to Shp2--

  *** xGab1[Ab]IP Yphos[pYAb] is increased irt Hgf (times)
    *** cells: MDCK<xGab1> in BMLS
    *** times: 0 15+ 30+ 60+ min
    *** unaffected by: xGab1(mnr)"delShp2" [substitution]
    *** source: 11046147-Fig-7b

requires Src--

  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: mEFs in BMLS
    *** bkg reqs: Src and/or Yes1 and/or Fyn [KO]
    *** source: 12941962-Fig-1b


Gab1 features

  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: MDCK in BMLS
    *** inhibited by: xSrc(mnr)"KD" [addition]
    *** source: 12941962-Fig-2b
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: HEK293<xGab1> in BMLS
    *** enhanced by: xSrc [addition]
    *** inhibited by: xSrc(mnr)"KD" [addition]
    *** source: 12941962-Fig-2a
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: MDCK<xGab1> in BMLS
    *** unaffected by: xGab1(mnr)"delGrb2BD" [substitution]
    *** source: 12058075-Fig-4c
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: mEFs<Src~null><Yes1~null><Fyn~null> in BMLS
    *** enhanced by: xSrc [addition]
    *** source: 12941962-Fig-1c

Chemicals

  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Hgf (tnr)
    *** cells: MIMCD3 in BMS
    *** unaffected by: U0126 [pretreatment]
    *** source: 11445578-Fig-4c
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: MDCK in BMLS
    *** inhibited by: PP1 [cotreatment]
    *** source: 12941962-Fig-2c


No extras

  *** Gab1[Ab] Yphos[pYAb-ppt] is increased irt Hgf (times)
    *** cells: MDCK in BMLS
    *** times: 0 2+ 5+ 15+ 50+ min
    *** source: 9252406-Fig-7a
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: MDCK<xGab1> in BMLS
    *** source: 10022866-Fig-1a
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Hgf (times)
    *** cells: MDCK<xGab1> in BMLS
    *** times: 0 1+ 3+ 15+ 30+ 60+ 120+ min
    *** source: 10022866-Fig-3b
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: A549 in BMLS
    *** source: 10618718-Fig-3b
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: HELA in BMLS
    *** source: 10618718-Fig-3d
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Hgf (2 min)
    *** cells: HEK293 in BMS
    *** source: 10753869-Fig-3d
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Hgf (times)
    *** cells: HEK293<xGab2><xPik3r1> in BMS
    *** times: 0+ 5++ 10++ 30++ min
    *** source: 10871282-Fig-3b
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Hgf (times)
    *** cells: HEK293T in BMS
    *** times: 0 10++ 20++ 30++ 60+ 90+ 120+ min
    *** comment: decrease at 60-120 min is reversed itpo xEnd3(mnr)"SH3"
    *** source: 11894096-Fig-4a
  *** Gab1[Ab]IP Yphos[pYAb] is increased irt Hgf (times)
    *** cells: MDCK in BMLS
    *** times: 0 5+ 15+ 60+ 120 min
    *** source: 12058075-Fig-1a
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: MDCK<xGab1> in BMLS
    *** source: 12058075-Fig-2b
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Hgf (times)
    *** cells: MDCK<xGab1> in BMLS
    *** times: 0 15+ 90+ min
    *** source: 12058075-Fig-2a
  *** xGab1[tAb]IP Yphos[pYAb] is increased irt Hgf (15 min)
    *** cells: HEK293<xGab1> in BMLS
    *** source: 12941962-Fig-3a

