===============================
Recruitment to the EgfR complex
===============================

  *** Src[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (2 min)
    *** cells: A7R5 in BMLS
    *** unaffected by: Herbimycin [addition]
    *** source: 11907028(D)
  *** Src[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** source: 14963038-Fig-7a
  *** EgfR[Ab] copptby[WB] Src[Ab] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 5++ 15++ 30+ 60+++ 120+++ min
    *** source: 10358079-Fig-5b
  *** Src[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (10 min)
    *** cells: DLD1 in BMS
    *** source: 7797556-Fig-6
  *** Src[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (10 min)
    *** cells: LS174T in BMS
    *** source: 7797556-Fig-6
  *** Src[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (10 min)
    *** cells: MCF7 in BMS
    *** source: 7797556-Fig-6
  *** Src[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (10 min)
    *** cells: NIH3T3 in BMS
    *** source: 7797556-Fig-6
  *** Src[Ab] copptby[WB] EgfR[Ab] is increased irt Egf (10 min)
    *** cells: WIDR in BMS
    *** source: 7797556-Fig-6
  *** EgfR[Ab] copptby[WB] Src[Ab] is detectable
    *** cells: MDAMB468 in BMLS "treated with Egf for 10 min"
    *** source: 7506422-Fig-4

contradiction

  *** Src[Ab] copptby[WB] EgfR[Ab] is unchanged irt Egf (10 min)
    *** cells: A431 in BMS
    *** source: 7797556-Fig-6

State in cells with "hyperactive Stat3,EgfR,Src"

  *** EgfR[Ab] copptby[WB] Src[Ab] is detectable
    *** cells: COLO357 in BMS
    *** source: 21573184-Fig-1a
  *** EgfR[Ab] copptby[WB] Src[Ab] is detectable
    *** cells: PANC1 in BMS
    *** source: 21573184-Fig-1a
  *** Src[Ab] copptby[WB] EgfR[Ab] is detectable
    *** cells: COLO357 in BMS
    *** source: 21573184-Fig-1a
  *** Src[Ab] copptby[WB] EgfR[Ab] is detectable
    *** cells: PANC1 in BMS
    *** source: 21573184-Fig-1a

No Coppt in other cells

  *** Src[Ab] copptby[WB] EgfR[Ab] is undetectable
    *** cells: A549 in BMS
    *** source: 21573184-Fig-S1c
  *** Src[Ab] copptby[WB] EgfR[Ab] is undetectable
    *** cells: MDAMB231 in BMS
    *** source: 21573184-Fig-S1c

=================================
Src activation requires Rala{GTP}
=================================
note: this requirement was removed from rule to make things fit
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

  *** Src[Ab]IP IVKA(Enolase)[32P-ATP] is increased irt Egf (10 min)
    *** cells: 3Y1<xEgfR> in BMLS
    *** inhibited by: xRala(S28N)"DN" [addition]
    *** source: 10675331-Fig-3b
  *** Src[Ab]IP IVKA(Enolase)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: xRala(S28N)"DN" [addition]
    *** source: 10675331-Fig-3a

=========================================================
Src activation/phos requires Shp2 phosphatase activity
=========================================================

  *** Src[Ab]IP IVKA(auto)[32P-ATP] is increased irt Egf (10 min)
    *** cells: MDAMB468 in BMLS
    *** inhibited by: xShp2(C459S/D425A)"CIA" [addition]
    *** source: 14665621-Fig-6b
  *** Src[Ab]IP IVKA(Gab1)[32P-ATP] is increased irt Egf (10 min)
    *** cells: MDAMB468 in BMLS
    *** inhibited by: xShp2(C459S/D425A)"CIA" [addition]
    *** source: 14665621-Fig-6b
  --------------------------------------------------------------
  *** Src[phosAb] phos(Y419)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Shp2~null><xShp2> in BMLS
    *** times: 0 5+ 15+ 30+ min
    *** bkg reqs: xShp2 [omission]
    *** source: 14967142-Fig-1a
  *** Src[phosAb] phos(Y419)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** bkg reqs: Shp2 [KO]
    *** source: 14967142-Fig-1a

============================================================
Src activation might require Hras{GTP} - more evidence needed
============================================================

  *** Src[Ab]IP IVKA(Enolase)[32P-ATP] is increased irt Egf (10 min)
    *** cells: 3Y1<xEgfR> in BMLS
    *** inhibited by: xHras(S17N)"DN" [addition] no-bkg-control
    *** source: 10675331-Fig-3c

======================
activation - no extras
======================

  *** Src[Ab]IP IVKA(Enolase)[32P-ATP] is increased irt Egf (5 min)
    *** cells: mEFs<Shp2~null><xShp2> in BMLS
    *** source: 14967142-Fig-1c
  *** Src[Ab]IP IVKA(auto)[32P-ATP] is increased irt Egf (times)
    *** cells: T47D in BMLS
    *** times: 0 1+ 2+ 5+ 10 min
    *** source: 11994282-Fig-5a
  *** Src[Ab]IP IVKA(Enolase)[32P-ATP] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 5 15 30+ 60++ 120+ min
    *** source: 10358079-Fig-5a
  *** Src[Ab]IP IVKA(auto)[32P-ATP] is increased irt Egf (10 min)
    *** cells: MDAMB468 in BMLS
    *** source: 14665621-Fig-6a
  *** Src[Ab]IP IVKA(Gab1)[32P-ATP] is increased irt Egf (10 min)
    *** cells: MDAMB468 in BMLS
    *** source: 14665621-Fig-6a
  *** Src[Ab]IP IVKA(Enolase)[32P-ATP] is increased irt Egf (10 min)
    *** cells: 3Y1<xEgfR> in BMLS
    *** source: 10675331-Fig-2b
  *** Src[Ab]IP IVKA(Enolase)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293 in BMLS
    *** source: 10675331-Fig-2a

==========
phos(Y419)
==========

  *** Src[phosAb] phos(Y419)[phosAb] is increased irt Egf (15 min)
    *** cells: A431 in BMS
    *** source: 14963038-Fig-7b
  *** Src[phosAb] phos(Y419)[phosAb] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0+ 5+ 10++ 30+ min
    *** source: 14613929-Fig-2a
  *** Src[phosAb] phos(Y419)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1++ 2++ 5+ min
    *** partially inhibited by: Forskolin [addition]
    *** unaffected by: xCsk [addition]
    *** unaffected by: xCsk(mnr)"SH3-SH2" [addition]
    *** source: 12606547-Fig-5e
  *** Src[phosAb] phos(Y419)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1++ 2++ 5+ min
    *** partially inhibited by: Forskolin [addition]
    *** source: 12606547-Fig-5c
  *** Src[phosAb] phos(Y419)[phosAb] is increased irt Egf (5 min)
    *** cells: HEK293T in BMLS
    *** source: 11598209-Fig-5c

contradiction

  *** Src[phosAb] phos(Y416)[phosAb] is detectable-but unchanged irt Egf (30 min)
    *** cells: MDAMB468 in BMLS
    *** unaffected by: AG490 [pretreatment] no-bkg-control
    *** source: 15950906-Fig-S3

=====================
phos(Y530) (aka Y529) in Pxn associated fraction
=====================

  *** Src[phosAb] phos(Y530)[phosAb] is decreased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0+++ 2+++ 5++ 10+ min
    *** source: 15831486-Fig-4a
  --------------- decreased in Src in Pxn-IP --------------------------------
  *** Src[Ab]IP phos(Y530)[phosAb] is decreased irt Egf (10 min)
    *** cells: MDAMB468 in BMLS
    *** comment: Src IP is with anti-Pxn Ab
    *** source: 14665621-Fig-7a
  *** Src[Ab]IP phos(Y530)[phosAb] is decreased irt Egf (10 min)
    *** cells: MDAMB468 in BMLS
    *** comment: Src IP is with anti-Pxn Ab
    *** inhibited by: xGab1(Y627F/Y659F) [addition]
    *** source: 14665621-Fig-7c
  --------------- no changed in total Src --------------------------------
  *** Src[phosAb] phos(Y530)[phosAb] is unchanged irt Egf (times)
    *** cells: mEFs<Shp2~null><xShp2> in BMLS
    *** times: 0 5 15 30 min
    *** source: 14967142-Fig-1a
  *** Src[phosAb] phos(Y530)[phosAb] is unchanged irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** source: 14967142-Fig-1a
  *** Src[phosAb] phos(Y530)[phosAb] is unchanged irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** source: 14967142-Fig-3b
  *** Src[Ab]IP phos(Y530)[phosAb] is unchanged irt Egf (10 min)
    *** cells: MDAMB468 in BMLS
    *** source: 14665621-Fig-7b

=====================
physical interactions
=====================

Muc1 coppt

  *** Src[Ab] copptby[WB] Muc1[Ab] is increased irt Egf (5 min)
    *** cells: ZR751 in BMS
    *** source: 11483589-Fig-4b
  *** Src[Ab] copptby[WB] xMuc1[Ab] is increased irt Egf (5 min)
    *** cells: HCT116 in BMS
    *** inhibited by: xMuc1(Y46F) [substitution]
    *** source: 11483589-Fig-4c


=============
other effects
=============

  *** Src[Ab]IP IVKA(Enolase)[32P-ATP] is increased irt Egf (5 min)
    *** cells: COS1 in BMLS
    *** inhibited by: xDok2 [addition]
    *** source: 15831486-Fig-3


