===================
Yphos or phos(Y705)
===================

Jak3--

  *** Stat3[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3~null><Lck~null><xJak3> in BMS
    *** reqs: xJak3 [omission]
    *** source: 10825200-Fig-4e

Syk--

  *** Stat3[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: hPHAblasts in BMS
    *** source: 7568001-Fig-2c
  *** Stat3[Ab]IP Yphos[pYAb] is increased irt IL2 (15 min)
    *** cells: NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3~null><Lck~null><xJak3> in BMS
    *** unaffected by: xSyk [addition]
    *** unaffected by: xSyk(mnr)"KD" [addition]
    *** source: 10825200-Fig-4e

Chemicals

  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt IL2 (10 min)
    *** cells: hThymicBlasts in BMS "IL2-deprived"
    *** unaffected by: Rapamycin [pretreatment] no-bkg-control
    *** unaffected by: LY294002 [pretreatment] no-bkg-control
    *** unaffected by: Wortmannin [pretreatment] no-bkg-control
    *** unaffected by: PD98059 [pretreatment] no-bkg-control
    *** source: 12681450-Fig-5b

No extras

  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt IL2 (times)
    *** cells: hThymicBlasts in BMS "IL2-deprived"
    *** times: 0 10+ 20+ 30+ 60+ min
    *** source: 12681450-Fig-3a
  *** Stat3[phosAb] phos(Y705)[phosAb] is increased irt IL2 (15 min)
    *** cells: hPBTCs<Itgb2~pos><Cd8~pos><Cd56~neg><Cd4~neg><Cd20~neg> in BMS
    *** unaffected by: IL12 [cotreatment]
    *** source: 10201984-Fig-4b

Contradictions

  *** Stat3[Ab]IP Yphos[pYAb] is unchanged irt IL2 (15 min)
    *** cells: KIT225 in BMS
    *** source: 7988557-Fig-8a
  *** Stat3[Ab]IP Yphos[pYAb] is unchanged irt IL2 (15 min)
    *** cells: KIT225 in BMLS "IL2-deprived"
    *** source: 7760829-Fig-4a

==========
phos(S727)
==========

Chemicals

  *** Stat3[Ab]IP phos(Y705)[phosAb] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** unaffected by: PD98059 [pretreatment]
    *** source: 9305919-Fig-7a
  *** Stat3[phosAb] phos(S727)[phosAb] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** inhibited by: PD98059 [pretreatment]
    *** source: 9305919-Fig-7a
  *** Stat3[phosAb] phos(S727)[phosAb] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** unaffected by: H7 [pretreatment]
    *** source: 9305919-Fig-7a

No extras

  *** Stat3[Ab] phos(S727)[phosAb-ppt] is increased irt IL2 (10 min)
    *** cells: hIL2RTCs in BMS "IL2-deprived"
    *** source: 9305919-Fig-4a
  *** Stat3[Ab] phos(S727)[phosAb-ppt] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** source: 9305919-Fig-6b
  *** Stat3[Ab]IP phos(S727)[phosAb] is increased irt IL2 (10 min)
    *** cells: hThymicBlasts in BMS "IL2-deprived"
    *** source: 12681450-Fig-3c
  *** Stat3[Ab]IP phos(S727)[phosAb] is unchanged irt IL2 (10 min)
    *** cells: hThymicBlasts in BMS "IL2-deprived"
    *** source: 12681450-Fig-5a
  *** Stat3[Ab]IP phos(Y705)[phosAb] is increased irt Ifna (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** source: 9305919-Fig-2
  *** Stat3[Ab]IP phos(Y705)[phosAb] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** source: 9305919-Fig-2
  *** Stat3[Ab]IP phos(Y705)[phosAb] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** unaffected by: H7 [pretreatment]
    *** source: 9305919-Fig-7a
  *** Stat3[phosAb] phos(S727)[phosAb] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** source: 9305919-Fig-6a


=====================
Total Phosphorylation
=====================

  *** Stat3[Ab]IP phos[WBMS] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** source: 9305919-Fig-3a


====================
DNA-binding activity
====================

  *** Stat3[Ab] oligo-binding[EMSA] is detectable-but unchanged irt IL2 (7 min)
    *** cells: YT in BMS "nuclear-extracts"
    *** oligo: GAS 5-GCCTGATTTCCCCGAAATGACGG-3
    *** source: 7479881-Fig-2d
  *** Stat3[Ab] oligo-binding[EMSA] is increased irt IL2 (10 min)
    *** cells: ED40515<xIL2Rg> in BMS
    *** oligo: GAS 5-GCCTGATTTCCCCGAAATGATGA-3
    *** source: 10421845-Fig-2d
  *** Stat3[Ab] oligo-binding[EMSA] is increased irt IL2 (15 min)
    *** cells: hPHAblasts in BMS
    *** oligo: "GRR" 5-ACGATGTTTCAAGGATTTGAGATGTATTTCCCAGAAAAG-3
    *** source: 7568001-Fig-3a
  *** Stat3[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (10 min)
    *** cells: hIL2RTCs in BMS "IL2-deprived"
    *** oligo: "Siem67" 5-GTCGACATTTCCCGTAAATC-3
    *** comment: coppt Stat3 is phosed on S727 [phosAb]
    *** comment: coppt Stat3 is phosed on Y705 [phosAb]
    *** source: 9305919-Fig-4b
  *** Stat3[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (10 min)
    *** cells: hIL2RTCs in BMS "IL2-deprived"
    *** oligo: "Siem67" 5-GTCGACATTTCCCGTAAATC-3
    *** source: 9305919-Fig-6c
  *** Stat3[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** oligo: GAS 5-GTATTTCCCAGAAAAGGAAC-3
    *** source: 10602027-Fig-4
  *** Stat3[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (times)
    *** cells: hIL2RTCs in BMS "IL2-deprived"
    *** times: 0 1+ 3++ 5++ 10+++ 15++ 30++ 60++ min
    *** oligo: "Siem67" 5-GTCGACATTTCCCGTAAATC-3
    *** source: 9305919-Fig-1a

Contradictions

  *** Stat3[Ab] snaggedby[WB] oligo[biotin] is unchanged irt IL2 (15 min)
    *** cells: hPHAblasts in BMS
    *** oligo: "FcgR-GAS" 5-GTATTTCCCAGAAAAGGAAC-3
    *** source: 7988557-Fig-9c
  *** Stat3[Ab] oligo-binding[EMSA] is unchanged irt IL2 (20 min)
    *** cells: NKL in BMS "IL2-deprived"
    *** source: 12372421-Fig-4b

Chemicals

  *** Stat3[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (10 min)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** unaffected by: PD98059 [pretreatment]
    *** source: 9305919-Fig-5b
  *** Stat3[Ab] snaggedby[WB] oligo[biotin] is increased irt IL2 (times)
    *** cells: KIT225 in BMS "IL2-deprived"
    *** times: 0 3+ 5++ 10++ 15++ min
    *** partially inhibited by: H7 [pretreatment]
    *** source: 9305919-Fig-7b
