===============
Kinase Activity
===============

reqs Mekk1--

  *** Jnk1[Ab]IP IVKA(Jun)[32P-ATP] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5++ 10++ 30++ 60+ min
    *** partially inhibited by: kMekk1(1-1188)"del-kinase-domain" [addition]
    *** source: 17143273-Fig-3

reqs Traf2--

  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60++ min
    *** reqs: Traf2 [KO]
    *** source: 18635759-Fig-1a
  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60++ min
    *** reqs: Traf2 [KO]
    *** source: 18635759-Fig-S1b
  *** Jnk1[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Cd40L (20 min)
    *** cells: mEFs<Traf2~null><xTraf2> in BMS
    *** partially reqs: xTraf2 [omission]
    *** source: 16260598-Fig-5c
  *** Jnk1[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Cd40L (times)
    *** cells: mEFs in BMS
    *** times: 0 20++ 30+ min
    *** reqs: Traf2 [KO]
    *** source: 16260598-Fig-4b
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: CH12LX in BMS
    *** times: 0 5+ 10++ 15+ 30 min
    *** reqs: Traf2 [null-mut]
    *** source: 12958312-Fig-6a
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: CH12LX<Traf2~null><xTraf2> in BMS
    *** times: 0 5+ 10++ 15+ 30 min
    *** reqs: xTraf2 [omission]
    *** source: 12958312-Fig-6b

does not req Traf3--

  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60++ min
    *** does not req: Traf3 [KO]
    *** comment: signal is enhanced at 5 min in Traf3-KO cells
    *** source: 18635759-Fig-1a
  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60++ min
    *** does not req: Traf3 [KO]
    *** comment: signal is enhanced at 5 min in Traf3-KO cells
    *** source: 18635759-Fig-S1b


reqs Traf6--

  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60++ min
    *** partially reqs: Traf6 [KO]
    *** source: 18635759-Fig-1a
  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60++ min
    *** partially reqs: Traf6 [KO]
    *** source: 18635759-Fig-S1b
  *** Jnk1[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Cd40L (20 min)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** reqs: xTraf6 [omission]
    *** source: 16260598-Fig-7b
  *** Jnk1[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Cd40L (times)
    *** cells: mEFs in BMS
    *** times: 0 20++ 30+ min
    *** reqs: Traf6 [KO]
    *** source: 16260598-Fig-6d
  *** Jnk1[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Cd40L (tnr)
    *** cells: HELA<xCd40(Q234A/E235A)"mT6"> in BMS
    *** reqs: Traf6 [RNAi]
    *** source: 16260598-Fig-8f

Chemicals

  *** Jnk1[Ab]IP IVKA(Jun)[32P-ATP] is increased irt anti-Cd40 (5 min)
    *** cells: M1241<xCd40> in BMS
    *** partially inhibited by: BaptaAM [pretreatment]
    *** inhibited by: TPEN [pretreatment]
    *** source: 10748139-Fig-5c

===========================
Phosphorylation on TPY site
===========================

Ciap1-- and/or Ciap2--

  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (10 min)
    *** cells: KMS28BM<Ciap1~null><Ciap2~null><xCiap1> in BMS
    *** reqs: xCiap2 [omission]
    *** inhibited by: xCiap2(mnr)"E3-ligase-inactive" [substitution]
    *** source: 18635759-Fig-7a
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: KMS28BM in BMS
    *** times: 0+ 5++ 10++ 30++ 60+ min
    *** partially reqs: Ciap1 and/or Ciap2 [null-mut]
    *** source: 18635759-Fig-S8
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: A20<Traf3~null> in BMS
    *** times: 0 10+ 30+ min
    *** unaffected by: SmacMimetic [addition]
    *** source: 18635759-Fig-7b
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 10+ 30+ min
    *** inhibited by: SmacMimetic [addition]
    *** source: 18635759-Fig-6a
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Tnf (times)
    *** cells: mEFs in BMS
    *** times: 0 2 5+ 10++ 30+ min
    *** partially inhibited by: SmacMimetic [addition]
    *** source: 18635759-Fig-S11

Cd40--

  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (5 min)
    *** cells: mSpCs<Cd40~null><xCd40> in BMS
    *** unaffected by: xCd40(del(230-247))"dTR6" [substitution]
    *** inhibited by: xCd40(T255A)"dTR23" [substitution]
    *** inhibited by: xCd40(del(230-247)/T255A)"dTRs" [substitution]
    *** source: 12354380-Fig-7b
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (5 min)
    *** cells: mSpCs<Cd40~pos> in BMS
    *** source: 12354380-Fig-7b

Mekk1--

  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60 min
    *** partially inhibited by: kMekk1(1-1188)"del-kinase-domain" [addition]
    *** source: 17143273-Fig-3
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Cd40L (times)
    *** cells: mSpBCs in BMS
    *** times: 0 10++ 30+ 60 min
    *** inhibited by: kMekk1(1-1188)"del-kinase-domain" [addition]
    *** source: 17143273-Fig-S5a

Nemo--

  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: 70Z3 in BMS
    *** times: 0 5+ 10+ 30++ 60++ min
    *** reqs: Nemo [KO]
    *** source: 18635759-Fig-2b


Traf2--

  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS
    *** times: 0 2 5+ 10++ 30++ min
    *** reqs: Traf2 [RNAi]
    *** source: 18635759-Fig-1b
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** reqs: Traf2 [KO]
    *** source: 18635759-Fig-1a
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** reqs: Traf2 [KO]
    *** source: 18635759-Fig-S1b
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Cd40L (10 min)
    *** cells: EJ in BMS
    *** reqs: Traf2 [RNAi]
    *** source: 16260598-Fig-3c
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Cd40L (times)
    *** cells: HELA<xCd40> in BMS
    *** times: 0 20++ 30+ min
    *** inhibited by: xCd40(T254A)"mT2/T3" [substitution]
    *** inhibited by: xCd40(Q234A/E235A/Q263A/T254A)"mT2/T3/T6" [substitution]
    *** source: 16260598-Fig-2c

not Traf3--

  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS
    *** times: 0 2 5+ 10++ 30++ min
    *** does not req: Traf3 [RNAi]
    *** comment: signal is enhanced at 5 min in Traf3-KO cells
    *** source: 18635759-Fig-1b
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** does not req: Traf3 [KO]
    *** comment: signal is enhanced at 5 min in Traf3-KO cells
    *** source: 18635759-Fig-1a
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** does not req: Traf3 [KO]
    *** source: 18635759-Fig-S1b
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Cd40L (15 min)
    *** cells: hUVECs in BMS
    *** unaffected by: xTraf3 [addition]
    *** source: 11714736-Fig-5a
  *** Jun[phosAb] phos(sitenr)[phosAb] is increased irt Cd40L (15 min)
    *** cells: hUVECs in BMS
    *** unaffected by: xTraf3 [addition]
    *** source: 11714736-Fig-5a

Traf6--

  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS
    *** times: 0 2 5+ 10++ 30++ min
    *** partially reqs: Traf6 [RNAi]
    *** source: 18635759-Fig-1b
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** partially reqs: Traf6 [KO]
    *** source: 18635759-Fig-1a
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 5+ 10++ 30++ 60+ min
    *** partially reqs: Traf6 [KO]
    *** source: 18635759-Fig-S1b
 --------- contradiction -------------------------
  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Cd40L (times)
    *** cells: HELA<xCd40> in BMS
    *** times: 0 20++ 30+ min
    *** unaffected by: xCd40(Q234A/E235A)"mT6" [substitution]
    *** inhibited by: xCd40(Q234A/E235A/Q263A/T254A)"mT2/T3/T6" [substitution]
    *** unaffected by: xCd40(Q234A/E235A/Q263A)"mT3/T6" [substitution]
    *** source: 16260598-Fig-2c

Ubc13--

  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS
    *** times: 0 5+ 10+ 30++ 60 min
    *** reqs: Ubc13 [RNAi]
    *** source: 18635759-Fig-2a

===================================
Requirement for Proteasome Activity
===================================

  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 10+ 30+ min
    *** inhibited by: MG132 [addition]
    *** source: 18635759-Fig-6b

=========================================
Jnks are not recruited to Cd40 irt Cd40L
=========================================

  *** Jnks[Ab] copptby[WB] Cd40[Ab] is unchanged irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS "P100-fraction"
    *** times: 0 2 5 10 min
    *** source: 18635759-Fig-6e
  *** Jnks[Ab] copptby[WB] Cd40[Ab] is unchanged irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS "P100-fraction"
    *** times: 0 10+ 30 min
    *** source: 18635759-Fig-6c

====================================================
Jnks coppt with Mekk1 after Mekk1 returns from Cd40
====================================================

  *** Jnks[Ab] copptby[WB] Mekk1[Ab] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS "S100-fraction"
    *** times: 0 5 10+ 30+ min
    *** comment: signal is enhanced at 5 min in Traf3-KO cells
    *** source: 18635759-Fig-S12
  *** Jnks[Ab] copptby[WB] Mekk1[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS "S100-fraction"
    *** times: 0 2 5 10+ min
    *** comment: coppt Jnks are phosed on TPY [phosAb]
    *** inhibited by: SmacMimetic [addition]
    *** source: 18635759-Fig-S9
  *** Jnks[Ab] copptby[WB] Mekk1[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS "S100-fraction"
    *** times: 0 10+ 30+ min
    *** comment: coppt Jnks are phosed on TPY [phosAb]
    *** source: 18635759-Fig-6c

