===============
Kinase Activity
===============

Cdc42--

  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** inhibited by: xCdc42(S17N)"DN" [addition]
    *** source: 14612425-Fig-5b

inhibited by Erbin

  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** comment: unaffected by addition Smad2[Ab]IP from MV1LU in BMS to IVKA
    *** comment: inhibited by addition Erbin[Ab]IP from MV1LU in BMS to IVKA
    *** source: 19289088-Fig-3b
  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** inhibited by: xErbin [addition]
    *** source: 19289088-Fig-4a
  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** inhibited by: xErbin [addition]
    *** source: 19289088-Fig-5a
  *** xPak2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B<xPak2> in BMLS
    *** comment: unaffected by addition Smad2[Ab]IP from MV1LU in BMS to IVKA
    *** comment: inhibited by addition Erbin[Ab]IP from MV1LU in BMS to IVKA
    *** source: 19289088-Fig-3d
  *** xPak2[tAb]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B<xPak2> in BMLS
    *** inhibited by: xErbin [addition]
    *** source: 19289088-Fig-5b

Fak1--

  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** reqs: xFak1 [omission]
    *** source: 21454615-Fig-2b

not Hras--

  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb2 (60 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: Hras(S17N)"DN" [addition]
    *** source: 17440079-Fig-5a

Pikrs--

  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (120 min)
    *** cells: COS7 in BMLS
    *** reqs: Pik3r? [AntiSense]
    *** source: 16288034-Fig-5d

Rac1--

  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** inhibited by: xRac1(S17N)"DN" [addition]
    *** source: 14612425-Fig-5b

not Rhoa--

  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: xRhoa(S17N)"DN" [addition]
    *** source: 14612425-Fig-5b

not Smad2-- and/or Smad3--

  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Smad2~null> in BMLS
    *** source: 14612425-Fig-3
  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Smad3~null> in BMLS
    *** unaffected by: xSmad2(S467A)"DN" [addition]
    *** source: 14612425-Fig-3

not Src-- Yes-- or Fyn--

  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (4 hr)
    *** cells: mEFs<Src~null><Yes1~null><Fyn~null> in BMLS
    *** source: 21454615-Fig-5a

Pak2 features

  *** xPak2(mnr)"CA"[tAb]IP IVKA(MBP)[32P-ATP] is detectable-but unchanged irt Tgfb1 (45 min)
    *** cells: NMUMG<xPak2(mnr)"CA"> in BMLS
    *** source: 19289088-Fig-6c
  *** xPak2(mnr)"DN"[tAb]IP IVKA(MBP)[32P-ATP] is unchanged irt Tgfb1 (45 min)
    *** cells: NMUMG<xPak2(mnr)"DN"> in BMLS
    *** source: 19289088-Fig-6c
  *** xPak2(T402E)[tAb]IP IVKA(MBP)[32P-ATP] is detectable-but unchanged irt Tgfb1 (45 min)
    *** cells: AKR2B<xPak2> in BMLS
    *** unaffected by: xErbin [addition]
    *** source: 19289088-Fig-5b
  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: xPak2 [addition]
    *** inhibited by: xPak2(mnr)"DN" [addition]
    *** source: 19289088-Fig-S1a
  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** inhibited by: Pak2(K278R)"KD" [addition]
    *** source: 14612425-Fig-6c
  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** inhibited by: xPak2(K278R)"KD" [addition]
    *** source: 14612425-Fig-4
  *** xPak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B<xPak2> in BMLS
    *** inhibited by: xPak2(K278R)"KD" [substitution]
    *** source: 14612425-Fig-4
  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 20+ 40+ 60++ 120++ min
    *** inhibited by: LY294002 [pretreatment]
    *** source: 16288034-Fig-5a

chemicals

  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** inhibited by: ToxinB [pretreatment]
    *** source: 14612425-Fig-5b
  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (120 min)
    *** cells: AKR2B in BMLS
    *** inhibited by: LY294002 [pretreatment]
    *** unaffected by: SH5 [pretreatment]
    *** source: 16288034-Fig-5b

no extras

  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (5 min)
    *** cells: AKR2B in BMLS
    *** source: 19289088-Fig-1c
  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** source: 19289088-Fig-1d
  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (4 hr)
    *** cells: AKR2B in BMLS
    *** source: 21454615-Fig-5a
  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** source: 14612425-Fig-1c
  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: BALB3T3 in BMLS
    *** source: 14612425-Fig-1c
  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: hFibros in BMLS
    *** source: 14612425-Fig-1c
  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: SWISS3T3 in BMLS
    *** source: 14612425-Fig-1c
  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is increased irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 5 15 30+ 60+ 120+ min
    *** source: 14612425-Fig-1a


===========================================
Pak2 activation does not occur in all cells
===========================================

A549

  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is unchanged irt Tgfb1 (45 min)
    *** cells: A549 in BMLS
    *** source: 19289088-Fig-6a

HELA

  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is unchanged irt Tgfb1 (45 min)
    *** cells: HELA in BMLS
    *** source: 14612425-Fig-1c

hPRECs

  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is unchanged irt Tgfb1 (45 min)
    *** cells: hPRECs in BMLS
    *** source: 14612425-Fig-1c

LLCPK1

  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is unchanged irt Tgfb1 (45 min)
    *** cells: LLCPK1 in BMLS
    *** source: 14612425-Fig-1c

MV1LU

  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is unchanged irt Tgfb1 (45 min)
    *** cells: MV1LU in BMLS
    *** unaffected by: xPak2 [addition]
    *** unaffected by: xPak2(mnr)"DN" [addition]
    *** source: 19289088-Fig-S1b
  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is unchanged irt Tgfb1 (45 min)
    *** cells: MV1LU in BMLS
    *** source: 14612425-Fig-1c
  *** Pak2[Ab]IP IVKA(MBP)[phosAb] is unchanged irt Tgfb1 (times)
    *** cells: MV1LU in BMLS
    *** times: 0 5 15 30 60 120 min
    *** source: 14612425-Fig-1b

NMUMG

  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is unchanged irt Tgfb1 (45 min)
    *** cells: NMUMG in BMLS
    *** source: 19289088-Fig-6c
  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (45 min)
    *** cells: NMUMG<Erbin~kd> in BMLS
    *** source: 19289088-Fig-6c
  *** Pak2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Tgfb1 (45 min)
    *** cells: NMUMG<Merlin~kd> in BMLS
    *** source: 19289088-Fig-6c

==============================================
Pak2 does not associate with TgfbR1 or TgfbR2
==============================================

  *** Pak2[Ab] copptby[WB] TgfbR1[Ab] is unchanged irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 5 15 30 60 min
    *** source: 14612425-Fig-2d
  *** Pak2[Ab] copptby[WB] TgfbR2[Ab] is unchanged irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 5 15 30 60 min
    *** source: 14612425-Fig-2d

==============================================
Pak2 is not phosphorylated by TgfbR1 or TgfbR2
==============================================

  *** rPak2[32P] phos[32P-ATP] is unchanged by TgfbR1[Ab]IP
    *** cells: none
    *** IPfrom: AKR2B in BMLS "treated with Tgfb1 for 60 min"
    *** source: 14612425-Fig-2c
  *** rPak2[32P] phos[32P-ATP] is unchanged by TgfbR2[Ab]IP
    *** cells: none
    *** IPfrom: AKR2B in BMLS "treated with Tgfb1 for 60 min"
    *** source: 14612425-Fig-2c













