=====================
Direct Ubiquitination
=====================

  *** Znf274[Ab]IP ubiq[UbAb] is increased by (rTraf6 + rUbe2l3 + rUbe1 + rUbiq)
    *** cells: none
    *** IPfrom: HEK293<xZnf274> in BMS
    *** inhibited by: rUbiq(K63R) [substitution]
    *** unaffected by: rUbiq(K6R/K11R/K29R/K33R/K48R)"K63-only" [substitution]
    *** source: 16252010-Fig-5c

=========================
Ubiquitination itpo Traf6
=========================

  *** xZnf274[Ab]IP ubiq[UbAb] is increased itpo xTraf6
    *** cells: HEK293<xZnf274><xUbiq> in BMS
    *** inhibited by: xZnf274(K19R) [substitution]
    *** source: 16252010-Fig-6a
  *** xZnf274[Ab]IP ubiq[tUbAb] is increased itpo xTraf6
    *** cells: HEK293<xZnf274><xUbiq> in BMS
    *** source: 16252010-Fig-3

======================
Unbiquitination on K63
======================

  *** xZnf274[Ab]IP ubiq[tUbAb] is increased itpo xUbiq
    *** cells: HEK293<xZnf274> in BMS
    *** unaffected by: xUbiq(K29R) [substitution]
    *** unaffected by: xUbiq(K48R) [substitution]
    *** inhibited by: xUbiq(K63R) [substitution]
    *** source: 16252010-Fig-5a
  *** Znf274[Ab]IP ubiq[tUbAb] is increased itpo xUbiq
    *** cells: PC12 in BMS "treated with Ngf(del(9-13)) for 10 min"
    *** unaffected by: xUbiq(K29R) [substitution]
    *** unaffected by: xUbiq(K48R) [substitution]
    *** inhibited by: xUbiq(K63R) [substitution]
    *** source: 16252010-Fig-5b

======================
Interaction with Traf6
======================

  *** xTraf6[Ab] copptby[WB] xZnf274[Ab] is detectable
    *** cells: HEK293<xTraf6><xZnf274><xUbiq> in BMS
    *** unaffected by: xSqstm1 [addition]
    *** unaffected by: xSqstm1(mnr)"del-UBA" [addition]
    *** unaffected by: xSqstm1(mnr)"del-Nterm" [addition]
    *** source: 16252010-Fig-5d
  *** xTraf6[Ab] copptby[WB] xZnf274[Ab] is detectable
    *** cells: HEK293<xTraf6><xZnf274><xUbiq> in BMS
    *** inhibited by: xZnf274(K19R) [substitution]
    *** source: 16252010-Fig-6b
  *** Traf6[Ab] copptby[WB] Znf274[Ab] is detectable
    *** cells: WEHI231 in BMS
    *** source: 14960584-Fig-2b
  *** xTraf6[tAb] copptby[WB] xZnf274[tAb] is detectable
    *** cells: HEK293<xZnf274><xTraf6> in BMS
    *** inhibited by: xZnf274(161-833)"dN161" [substitution]
    *** inhibited by: xZnf274(241-833)"dN241" [substitution]
    *** unaffected by: xZnf274(1-520)"dC520" [substitution]
    *** unaffected by: xZnf274(1-383)"dC383" [substitution]
    *** source: 14960584-Fig-3c
  *** xTraf6[tAb] copptby[WB] xZnf274[tAb] is detectable
    *** cells: HEK293<xZnf274><xTraf6> in BMS
    *** source: 14960584-Fig-1b
  *** xZnf274[Ab] copptby[WB] xTraf6[tAb] is detectable
    *** cells: HEK293<xZnf274><xTraf6> in BMS
    *** inhibited by: xTraf6(mnr)"dTrafC" [substitution]
    *** inhibited by: xTraf6(mnr)"dCoil" [substitution]
    *** unaffected by: xTraf6(mnr)"dRing" [substitution]
    *** unaffected by: xTraf6(mnr)"DN" [substitution]
    *** source: 14960584-Fig-3d
  *** xZnf274[Ab] copptby[WB] xTraf6[tAb] is detectable
    *** cells: HEK293<xZnf274><xTraf6> in BMS
    *** source: 14960584-Fig-1b
  *** xZnf274[Ab] copptby[WB] xTraf6[tAb] is detectable
    *** cells: HEK293<xZnf274><xTraf6> in BMS
    *** source: 14960584-Fig-11
