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Association with Full Length Tpl2
=================================

required Nfkb1 features: 497-538 and L841A

  *** xTpl2[Ab] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xTpl2><xNfkb1> in BMS
    *** unaffected by: xNfkb1(del(801-892))"dDD" [substitution]
    *** unaffected by: xNfkb1(L841A) [substitution]
    *** unaffected by: xNfkb1(del(497-538)) [substitution]
    *** inhibited by: xNfkb1(del(497-538)/del(801-892)) [substitution]
    *** inhibited by: xNfkb1(del(497-538)/L841A) [substitution]
    *** source: 12832462-Fig-5c
  *** xTpl2[tAb] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xTpl2><xNfkb1> in BMS
    *** unaffected by: xNfkb1(del(801-892))"dDD" [substitution]
    *** unaffected by: xNfkb1(L841A) [substitution]
    *** unaffected by: xNfkb1(del(497-538)) [substitution]
    *** inhibited by: xNfkb1(del(497-538)/del(801-892)) [substitution]
    *** inhibited by: xNfkb1(del(497-538)/L841A) [substitution]
    *** source: 12832462-Fig-6c

required Tpl2 features:  D270 Kinase domain? or kinase activity?

  *** xTpl2[tAb] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xTpl2><xNfkb1> in BMS
    *** inhibited by: Tpl2(1-397/D270A)"KDdelC" [substitution]
    *** source: 12832462-Fig-1d
  *** xTpl2[tAb] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xTpl2><xNfkb1> in BMS
    *** unaffected by: Tpl2(1-397)"delC" [substitution]
    *** source: 12832462-Fig-1a
  *** xTpl2[tAb] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xTpl2><xNfkb1> in BMS
    *** unaffected by: Tpl2(30-397)"M30delC" [substitution]
    *** unaffected by: Tpl2(104-397)"Q104delC" [substitution]
    *** unaffected by: Tpl2(131-397)"P131delC" [substitution]
    *** source: 12832462-Fig-1c
  *** Nfkb1[Ab] copptby[WB] xTpl2[tAb] is detectable
    *** cells: RAW2647<xTpl2> in BMS
    *** unaffected by: xTpl2(S400A) [substitution]
    *** source: 17709378-Fig-6a

chemicals

  *** Tpl2[Ab] copptby[WB] Nfkb1[Ab] is detectable
    *** cells: HELA in BMS
    *** comment: both the long and short forms of TPl2 are coppt
    *** unaffected by: Piceatannol [addition]
    *** source: 16291755-Fig-5a
  *** Tpl2[Ab] copptby[WB] Nfkb1[Ab] is detectable
    *** cells: mBMMs in BMS
    *** comment: both the long and short forms of TPl2 are coppt
    *** unaffected by: Piceatannol [addition]
    *** source: 16291755-Fig-5a

no extras

  *** Nfkb1[Ab] copptby[WB] Tpl2[Ab] is detectable
    *** cells: HELAS3 in BMS
    *** source: 15169888-Fig-1c
  *** xTpl2[tAb] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xTpl2><xNfkb1> in BMS
    *** source: 15699325-Fig-2b
  *** Tpl2[Ab] copptby[WB] Nfkb1[Ab] is detectable
    *** cells: HELAS3 in BMS
    *** source: 15169888-Fig-1c
  *** Tpl2[Ab] copptby[WB] Nfkb1[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 9950430-Fig-3a
  *** Nfkb1[Ab] copptby[WB] Tpl2[Ab] is detectable
    *** cells: HELA in BMS
    *** source: 9950430-Fig-3a

=============================
Miscellaneous Bits and Pieces
=============================

  *** rTpl2[35S] boundby[WB] rNfkb1(808-892)[GST] is detectable
    *** cells: none
    *** source: 12832462-Fig-2c
  *** xTpl2[tAb] snaggedby[WB] rNfkb1(497-966)[GST] is detectable
    *** cells: HEK293<xTpl2> in BMS
    *** unaffected by: xTpl2(S400A) [substitution]
    *** source: 17709378-Fig-6a
  *** xNfkb1(del(801-892))[tAb] snaggedby[WB] rTpl2(398-467)"Cterm"[GST] is detectable
    *** cells: HEK293<xNfkb1(del(801-892))> in BMS
    *** inhibited by: xNfkb1(del(497-538)) [substitution]
    *** source: 12832462-Fig-5b
  *** xTpl2(1-397)"delC"[Ab] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xTpl2(1-397)><xNfkb1> in BMS
    *** unaffected by: xNfkb1(1-892) [substitution]
    *** inhibited by: xNfkb1(1-801) [substitution]
    *** inhibited by: xNfkb1(del(801-892))"dDD" [substitution]
    *** inhibited by: xNfkb1(L841A) [substitution]
    *** source: 12832462-Fig-2b
  *** xTpl2(1-397)"delC"[tAb] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xTpl2(1-397)><xNfkb1> in BMS
    *** inhibited by: xNfkb1(del(801-892))"dDD" [substitution]
    *** inhibited by: xNfkb1(del(497-538)) [substitution]
    *** source: 12832462-Fig-6d
  *** rNfkb1[35S] boundby[WB] rTpl2[Ab] is detectable
    *** cells: none
    *** inhibited by: rNfkb1(1-880)"p97" [substitution]
    *** inhibited by: rNfkb1(1-777)"p85" [substitution]
    *** inhibited by: rNfkb1(1-545)"p60" [substitution]
    *** inhibited by: rNfkb1(1-504)"p55" [substitution]
    *** inhibited by: rNfkb1(1-435)"p48" [substitution]
    *** source: 9950430-Fig-1b
  *** rNfkb1[35S] boundby[WB] rTpl2[Ab] is detectable
    *** cells: none
    *** inhibited by: rTpl2(1-398)"delC" [substitution]
    *** source: 9950430-Fig-2b
  *** rNfkb1[35S] boundby[WB] rTpl2[Ab] is detectable
    *** cells: none
    *** unaffected by: rNfkb1(435-969)"delN" [substitution]
    *** source: 9950430-Fig-1c
  *** rNfkb1[35S] boundby[WB] rTpl2[Ab] is detectable
    *** cells: none
    *** unaffected by: rTpl2(30-468)"M30" [substitution]
    *** unaffected by: rTpl2(104-468)"Q104" [substitution]
    *** unaffected by: rTpl2(131-468)"P131" [substitution]
    *** source: 9950430-Fig-2b




