===============
Kinase Activity
===============

Akt1-- ???

  *** xBraf[tAb]IP IVKA(Erk1,Elk1)[32P-ATP] is increased irt Egf (3 min)
    *** cells: HEK293<xBraf> in BMLS
    *** bkg inhibited by: xAkt1(myr)"CA" [addition]
    *** source: 10869359-Fig-1a

Hras--

  *** xBraf[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7 in BMLS
    *** inhibited by: xHras(S17N)"DN" [addition]
    *** source: 9094716-Fig-5b,5c

IqGap1--

  *** Braf[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (10 min)
    *** cells: mEFs in BMLS
    *** reqs: IqGap1 [KO]
    *** source: 17563371-Fig-3a
  *** Braf[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (10 min)
    *** cells: mEFs in BMLS
    *** reqs: IqGap1 [RNAi]
    *** source: 17563371-Fig-4a

Mlk3--

  *** Braf[Ab]IP IVKA(auto)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293 in BMLS
    *** reqs: Mlk3 [RNAi]
    *** source: 16537381-Fig-1a
  *** Braf[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293 in BMLS
    *** reqs: Mlk3 [RNAi]
    *** source: 16537381-Fig-1a
  *** Braf[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293<Mlk3~null> in BMLS
    *** enhanced by: xMlk3 [addition]
    *** enhanced by: xMlk3(K144R)"KD" [addition]
    *** source: 16537381-Fig-1b
not Rap1a--

  *** xBraf[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (10 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xRap1a(T17N)"DN" [addition]
    *** source: 9094716-Fig-5b,5c

not Raf1--

  *** Braf[Ab]IP IVKA(Mek1,Erk?,MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** bkg repressed by: Raf1 [KO]
    *** source: 11296228-Fig-6a
  *** Braf[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2++ 5+++ 10+ 20+ 60 min
    *** repressed by: Raf1 [KO]
    *** source: 11296227-Fig-4d

chemicals

  *** Braf[Ab]IP IVKA(auto)[32P-ATP] is increased irt Egf (5 min)
    *** cells: PC12<xPdgfRb> in BMLS
    *** inhibited by: Forskolin [addition]
    *** source: 7935374-Fig-3a,3b,4a,4b,4c
  *** Braf[Ab]IP IVKA(Mek1,Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: PC12<xPdgfRb> in BMLS
    *** inhibited by: Forskolin [addition]
    *** source: 7935374-Fig-5a,5b,6a,6b
  *** Braf[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: PC12<xPdgfRb> in BMLS
    *** bkg inhibited by: Forskolin [addition]
    *** source: 7935374-Fig-2a,2b

===============
Phosphorylation
===============

phos(T119)

  *** Braf[phosAb] phos(T119)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 5+ 10+ min
    *** inhibited by: BaptaAM [addition]
    *** source: 16858395-Fig-5e

phos(S446)

  *** xBraf[phosAb] phos(S446)[phosAb] is detectable-but unchanged irt Egf (30 min)
    *** cells: HEK293 in BMLS
    *** source: 16803888-Fig-3b
  *** Braf[Ab]IP phos(S446)[phosAb] is detectable-but unchanged irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0+ 1+ 2+ 5+ 10+ 15+ min
    *** source: 15710605-Fig-1b


phos(T599/S602)

  *** Braf[phosAb] phos(T599/S602)[phosAb] is increased irt Egf (10 min)
    *** cells: CCD18CO in BMLS
    *** reqs: Mlk3 [RNAi]
    *** source: 15258589-Fig-3b
  *** Braf[phosAb] phos(T599/S602)[phosAb] is increased irt Egf (10 min)
    *** cells: WI38 in BMLS
    *** reqs: Mlk3 [RNAi]
    *** source: 15258589-Fig-3b

phos(T753)

  *** xBraf[tAb]IP Tphos[pTAb] is increased irt Egf (times)
    *** cells: COS1 in BMLS
    *** times: 0+ 5++ 30++ min
    *** inhibited by: xBraf(T753A) [substitution]
    *** source: 16508002-Fig-5d

STphos

  *** Braf[Ab]IP phos[32P-ATP] is increased irt Egf (20 min)
    *** cells: mEFs<Ksr1~null><xKsr1> in BMLS
    *** comment: phos sites were shown to be S151 T401 S750 T753 by HPLC
    *** comment: phos sites were inhibited by U0126 [pretreatment]
    *** source: 19541618-Fig-S3a
  *** Braf[Ab]IP STphos[pSTAb] is increased irt Egf (10 min)
    *** cells: mEFs<Ksr1~null><xKsr1><xMek1> in BMLS
    *** partially reqs: xKsr1 [omission]
    *** partially inhibited by: xKsr1(mnr)"AxAp" [substitution]
    *** source: 19541618-Fig-4b
  *** Braf[Ab]IP STphos[pSTAb] is increased irt Egf (20 min)
    *** cells: mEFs<Ksr1~null><xKsr1><xMek1> in BMLS
    *** inhibited by: U0126 [pretreatment]
    *** source: 19541618-Fig-4a
  *** Braf[Ab]IP STphos[pSTAb] is increased irt Egf (20 min)
    *** cells: mEFs<Ksr1~null><xKsr1><xMek1> in BMLS
    *** inhibited by: xKsr1(T260A/T274A/S320A/S443A)"hT272A/T286A/S197A/S455A" [substitution]
    *** source: 19541618-Fig-4a
  *** Braf[Ab]IP STphos[pSTAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 20+ min
    *** source: 19541618-Fig-4c
  *** Braf[Ab]IP STphos[pSTAb] is increased irt Egf (times)
    *** cells: mEFs<Ksr1~null><xKsr1><xMek1> in BMLS
    *** times: 0+ 10++ 20++ min
    *** comment: partially reqs xKsr1 [omission] at 10 min only
    *** source: 19541618-Fig-4b


=========
no extras
=========

  *** Braf[Ab]IP IVKA(Mek1,Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: bLUCs in BMLS
    *** source: 12644307-Fig-1a
  *** Braf[Ab]IP IVKA(Mek?,Erk2)[32P-ATP] is increased irt Egf (times)
    *** cells: mEFs in BMLS "immortalized"
    *** times: 0+ 2++ 5++ 10++ 20+++ 60+ min
    *** source: 15199148-Fig-1c
  *** Braf[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (5 min)
    *** cells: RAT1 in BMLS
    *** source: 9774335-Fig-3b
  *** Braf[Ab]IP IVKA(auto)[32P-ATP] is increased irt Egf (10 min)
    *** cells: PC12 in BMLS
    *** source: 9094716-Fig-3a
  *** Braf[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (10 min)
    *** cells: PC12 in BMLS
    *** source: 9094716-Fig-3a
  *** Braf[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Egf (times)
    *** cells: PC12 in BMS
    *** times: 0+ 1+ 2+ 5++ 10++ 20++ 45++ 90++ 180++ min
    *** source: 8647237-Fig-1c
  *** Braf[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: PC12 in BMLS
    *** source: 11278445-Fig-2
  *** Braf[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Egf (times)
    *** cells: PC12 in BMLS
    *** times: 0 2+ 5+++ 15++ 30+ 60 min
    *** source: 11278445-Fig-1b

=====================
Physical interactions
=====================

Hras--

  *** Braf[Ab] snaggedby[WB] rHras{GMPPNP}[GST] is increased irt Egf (20 min)
    *** cells: PC12 in BMS
    *** bkg inhibited by: rHras{GDP} [substitution]
    *** source: 9001246-Fig-3b

IqGap1--

  *** xBraf[Ab] copptby[WB] xIqGap1[tAb] is increased irt Egf (10 min)
    *** cells: HEK293H in BMS
    *** source: 18567582-Fig-9a

Ksr1--

  *** Braf[Ab] copptby[WB] Ksr1[Ab] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5++ 20+ min
    *** source: 19541618-Fig-4c
  *** Braf[Ab] copptby[WB] xKsr1[tAb] is increased irt Egf (times)
    *** cells: mEFs<Ksr1~null><xKsr1><xMek1> in BMLS
    *** unaffected by: U0126 [pretreatment]
    *** times: 0 5++ 20+ min
    *** source: 19541618-Fig-3b
  *** Braf[Ab] copptby[WB] xKsr1[tAb] is increased irt Egf (5 min)
    *** cells: mEFs<Ksr1~null><xKsr1> in BMS
    *** source: 2292131-Fig-1b

Raf1--

  *** Braf[Ab] copptby[WB] Raf1[Ab] is increased irt Egf (5 min)
    *** cells: COS1 in BMLS
    *** source: 16508002-Fig-1b

Rheb--

  *** Braf[Ab] snaggedby[WB] rRheb{GMPPNP}[GST] is detectable-but unchanged irt Egf (20 min)
    *** cells: PC12 in BMS
    *** inhibited by: rRheb{GDP} [substitution]
    *** source: 9001246-Fig-3b

