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Complex requires Nfkb1 phos sites
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S927

  *** rBtrc[35S] boundby[WB] rNfkb1(758-967){phos}[GST] is detectable
    *** cells: none
    *** comment: rNfkb1(758-967) was phosed by rIkk2
    *** comment: not detectable if rNfkb1(758-967) is not phosed
    *** partially inhibited by: rNfkb1((758-967)/S927A){phos} [substitution]
    *** unaffected by: rNfkb1((758-967)/S932A){phos} [substitution]
    *** inhibited by: rNfkb1((758-967)/S927A/S932A){phos} [substitution]
    *** source: 12482991-Fig-7a

S921A/S923A/S927A

  *** rBtrc[35S] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xNfkb1><xIkk2> in BMS
    *** comment: rBtrc was added to lysate before IP
    *** inhibited by: xNfkb1(1-850)"delC5" [substitution]
    *** inhibited by: xNfkb1(S921A/S923A/S927A) [substitution]
    *** source: 11158290-Fig-3b

sites phosed by Ikk2

  *** rBtrc[35S] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xNfkb1><xIkk2> in BMS
    *** comment: rBtrc was added to lysate before IP
    *** inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 11158290-Fig-3a
  *** rBtrc[35S] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xNfkb1><xIkk2> in BMS
    *** comment: rBtrc was added to lysate before IP
    *** inhibited by: xIkk2(mnr)"KD" [substitution]
    *** source: 11158290-Fig-3b
  *** rBtrc[35S] copptby[WB] xNfkb1[tAb] is detectable
    *** cells: HEK293<xNfkb1><xIkk2> in BMS
    *** comment: rBtrc was added to lysate before IP
    *** inhibited by: xNfkb1(1-850)"delC5" [substitution]
    *** source: 11158290-Fig-3a


