======================================
Traf6 ubiquitination irt IL1 requires:
======================================

Tab2--

  *** Traf6[Ab]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: mEFs in BMLS
    *** times: 0 5+ 10+ 20+ 30+ min
    *** reqs: Tab2 [KO]
    *** source: 15836773-Fig-1

not Ubc13--

  *** Traf6[Ab]IP ubiq[UbAb] is increased irt IL1 (20 min)
    *** cells: mEFs in BMS
    *** comment: lysates were boiled to remove noncovalently attached proteins before IP
    *** does not req: Ubc13 [KO]
    *** source: 19843958-Fig-4b

==========================
Traf6-Ubiq is K63 linked
==========================

  *** Traf6[Ab]IP ubiq[tUbAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1><xTLR4><xUbiq(K63only)> in BMS
    *** times: 0 2+ 5++ 10++ 20++ min
    *** source: 18347055-Fig-1a
  *** Traf6[Ab]IP ubiq[K63UbAb] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30+ 45++ 60+ 90+ 120 240 min
    *** comment: decrease after 45 min requires Tnfaip3 [KO]
    *** comment: decrease after 45 min is unaffected by CHX pretreatment (Fig-2c)
    *** source: 20185725-Fig-2a

==================================================
Traf6 features required for ubiquitination irt IL1
==================================================

  *** xTraf6[tAb]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: mEFs in BMLS
    *** times: 0+ 5++ 10+++ min
    *** inhibited by: xTraf6(C70A)"E3-mut" [substitution]
    *** inhibited by: xTraf6(K124R) [substitution]
    *** source: 17135271-Fig-7a
  *** xTraf6[Ab] upshift[WBMS] is increased irt IL1b (5 min)
    *** cells: mEFs<Traf6~null> in BMS
    *** inhibited by: xTraf6(C70A) [substitution]
    *** inhibited by: xTraf6(mnr)"K32-518R" [substitution]
    *** source: 19112497-Fig-3c

no extras--

  *** Traf6[Ab]IP ubiq[UbAb] is increased irt IL1b (5 min)
    *** cells: HEK293<xIL1R1><xTLR4> in BMS
    *** source: 18347055-Fig-1b
  *** Traf6[Ab] upshift[WBMS] is increased irt IL1b (times)
    *** cells: HELA in BMS
    *** times: 0 5++ 10++ 20++ 40+ 60 min
    *** source: 11460167-Fig-4a
  *** Traf6[Ab]IP ubiq[UbAb] is increased irt IL1b (times)
    *** cells: HELA in BMS
    *** times: 0 5++ 10++ 20++ 40++ 60+ min
    *** source: 11460167-Fig-4a

======================
Later Deubiquitination
======================

  *** Traf6[Ab]IP ubiq[UbAb] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30+ 45++ 60++ 90+ 120 240 min
    *** comment: decrease after 60 min requires Itch [KO]
    *** source: 20185725-Fig-S5e
  *** Traf6[Ab]IP ubiq[UbAb] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30+ 45++ 60++ 90+ 120 240 min
    *** comment: decrease after 60 min requires Tax1bp1 [KO]
    *** source: 20185725-Fig-S2a
  *** Traf6[Ab]IP ubiq[UbAb] is increased irt IL1 (times)
    *** cells: mEFs<Tnfaip3~null><xTnfaip3> in BMS
    *** times: 0 1+ 2 4 6 hr
    *** comment: decrease after 60 min reversed by xTnfaip3(C103A) [subsitution]
    *** comment: decrease after 60 min reversed by xTnfaip3(C624A/C627A) [subsitution]
    *** source: 20185725-Fig-S8a
  *** Ubiq[K63UbAb] copptby[WB] Traf6[Ab] is increased irt IL1 (times)
    *** cells: mBMMs in BMS
    *** times: 0 15+ 30+ 120 min
    *** comment: decrease after 30 min reqs Ikk1 [RNAi]
    *** source: 21765415-Fig-6f


