=============================================================
Rsk3 is directly phosphorylated on S227 but not S386 by Pdpk1
=============================================================

phos(S227)

  *** rRsk3(1-360)[32P] phos[32P-ATP] is increased by xPdpk1(51-556)[tAb]IP
    *** cells: none
    *** IPfrom: COS7<xPdpk1(51-556)> in BMLS
    *** inhibited by: rRsk3(1-360/S227E) [substitution]
    *** source: 10480933-Fig-2

not phos(S386)

  *** rRsk3[GST]IP phos(S386)[phosAb] is unchanged by rPdpk1(51-556)
    *** cells: none
    *** source: 10480933-Fig-10a

total phos

  *** rRsk3(1-373/K100A)[32P] phos[32P-ATP] is increased by xPdpk1(51-556)[tAb]IP
    *** cells: none
    *** IPfrom: COS7<xPdpk1(51-556)> in BMLS
    *** source: 10480933-Fig-2

===========================================================
Phosphorylation of Nterm kinase on S227 requires phos(S386)
===========================================================

  *** xRsk3(1-389)[tAb]IP phos(S227)[phosAb] is detectable
    *** cells: COS7<xRsk3> in BMLS
    *** inhibited by: xRsk3(1-380) [substitution]
    *** inhibited by: xRsk3(1-380/S386A) [substitution]
    *** source: 10856237-Fig-3b

===================================================================
Overexpression of Pdpk1 causes increased phos of both S227 and S386
===================================================================

phos(S386)

  *** xRsk3[tAb]IP phos(S386)[phosAb] is increased itpo xPdpk1
    *** cells: COS7<xRsk3> in BMLS
    *** source: 10480933-Fig-8b
  *** xRsk3[tAb]IP phos(S386)[phosAb] is increased itpo xPdpk1
    *** cells: COS7<xRsk3> in BMLS
    *** unaffected by: xRsk3(S369A) [substitution]
    *** partially inhibited by: xRsk3(S369A/T577A) [substitution]
    *** source: 10480933-Fig-9b

not phos(S369)

  *** xRsk3[tAb]IP phos(S369)[phosAb] is unchanged itpo xPdpk1
    *** cells: COS7<xRsk3> in BMLS
    *** unaffected by: xRsk3(S227E) [substitution]
    *** unaffected by: xRsk3(S386A) [substitution]
    *** source: 10480933-Fig-8b

total phos

  *** xRsk3(1-360)[tAb]IP phos[32Pi] is increased itpo xPdpk1(51-556)
    *** cells: COS7<xRsk3(1-360)> in BMLS
    *** inhibited by: xRsk3(1-360/S227E) [substitution]
    *** source: 10480933-Fig-4

=====================
Basal Phosphorylation
=====================

  *** xRsk3(1-389)[tAb]IP phos(S386)[phosAb] is detectable
    *** cells: COS7<xRsk3(1-389)> in BMLS
    *** inhibited by: xRsk3(1-380) [substitution]
    *** inhibited by: xRsk3(1-389/S386A) [substitution]
    *** inhibited by: xRsk3 [substitution]
    *** source: 10856237-Fig-3b
  *** xRsk3[tAb]IP phos(S386)[phosAb] is detectable
    *** cells: COS7<xRsk3> in BMLS
    *** enhanced by: xRsk3(S227E) [substitution]
    *** source: 10480933-Fig-8b

======================================================
Association between Rsk3 and Pdpk1 requires phos(S386)
======================================================

  *** rRsk3(373-395){phos} boundby[SPR] rPdpk1[GST] is detectable
    *** cells: none
    *** inhibited by: rRsk3(373-395) [substitution] 
    *** source: 12374740-Fig-3b

=====
other
=====

  *** rRsk3(373-395){phos} boundby[SPR] rPdpk1[GST] is detectable
    *** cells: none
    *** inhibited by: rPdpk1(R131A) [substitution]
    *** inhibited by: rPdpk1(R131M) [substitution]
    *** source: 12374740-Fig-3b
  *** xPdpk1[tAb] copptby[WB] xRsk3[tAb] is detectable
    *** cells: COS7<xPdpk1><xRsk3> in BMLS "treated with Egf for 35 min"
    *** inhibited by: xPdpk1(R131A) [substitution]
    *** inhibited by: xPdpk1(R75A) [substitution]
    *** inhibited by: xPdpk1(K76A) [substitution]
    *** inhibited by: xPdpk1(K77A) [substitution]
    *** source: 12374740-Fig-3a
