Tak1-ubiq is placed before Tak1-phos because of requirement for ubiq sites
and Traf6 for phos

===============
Kinase Activity
===============

Traf6--

  *** Tak1[Ab]IP IVKA(auto)[32P-ATP] is increased irt Tgfb1 (times)
    *** cells: mEFs in BMS
    *** times: 0 2 5+ 15++ min
    *** reqs: Traf6 [KO]
    *** source: 18758450-Fig-S5c

  *** xTak1[tAb]IP IVKA(P38?,Mek2)[32P-ATP] is increased irt Tgfb1 (10 min)
    *** cells: MC3T3E1 in BMS
    *** inhibited by: xTak1(K63W) [substitution]
    *** source: 8533096-Fig-4c
  *** xTak1[tAb]IP IVKA(P38?,Mek2)[32P-ATP] is increased irt Tgfb1 (times)
    *** cells: MC3T3E1 in BMS
    *** times: 0 5+ 10++ 30+ min
    *** source: 8533096-Fig-4a
  *** xTak1[tAb]IP IVKA(P38?,Mek2)[32P-ATP] is increased irt Tgfb1 (times)
    *** cells: MC3T3E1 in BMS
    *** times: 0 5+ 10++ min
    *** source: 8533096-Fig-4d

===============
Phosphorylation
===============

Tab1--

  *** Tak1[phosAb] phos(T187)[phosAb] is increased irt Tgfb1 (times)
    *** cells: mMesangialCells in BMLS
    *** times: 0 5++ 15+ min
    *** reqs: Tab1 [RNAi]
    *** source: 19556242-Fig-5c

Tak1--  kinase activity

  *** xTak1[phosAb] phos(T184)[phosAb] is increased itpo xTab1
    *** cells: mMesangialCells<xTak1> in BMS
    *** inhibited by: xTak1(K63W)"KD" [substitution]
    *** inhibited by: xTak1(T187A) [substitution]
    *** source: 19556242-Fig-5a
  *** xTak1[phosAb] phos(T187)[phosAb] is detectable
    *** cells: mMesangialCells<xTak1> in BMS
    *** inhibited by: xTak1(K63W)"KD" [substitution]
    *** source: 19556242-Fig-5b
  *** xTak1[phosAb] phos(T187)[phosAb] is detectable
    *** cells: mMesangialCells<xTak1><xTab1> in BMS
    *** unaffected by: xTak1(mnr)"del-Cterm" [substitution]
    *** source: 19556242-Fig-8e
  *** xTak1[phosAb] phos(T187)[phosAb] is increased itpo xTab1
    *** cells: mMesangialCells<xTak1> in BMS
    *** inhibited by: xTak1(K63W)"KD" [substitution]
    *** inhibited by: xTak1(T187A) [substitution]
    *** source: 19556242-Fig-5a

Tak1-- ubiq site

  *** xTak1[phosAb] phos(T187/S192)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HEK293T<xTak1><xUbiq> in BMS
    *** times: 0+ 2+ 5++ 15++ min
    *** inhibited by: xTak1(K34R) [substitution]
    *** source: 18758450-Fig-5f

Traf6--

  *** Tak1[phosAb] phos(T187)[phosAb] is increased irt Tgfb1 (times)
    *** cells: mMesangialCells in BMLS
    *** times: 0+ 5++ 15++ 30++ min
    *** reqs: Traf6 [RNAi]
    *** source: 19556242-Fig-7b
  *** xTak1[phosAb] phos(T187/S192)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HEK293T<xTak1><xUbiq> in BMS
    *** times: 0+ 2+++ 5++ 15+ min
    *** inhibited by: xTraf6(mnr)"DN" [substitution]
    *** source: 18758450-Fig-4b
  *** Tak1[phosAb] phos(T187/S192)[phosAb] is increased irt Tgfb1 (times)
    *** cells: PC3U in BMS
    *** times: 0 5+ 15++ 30++ 60++ min
    *** reqs: Traf6 [RNAi]
    *** source: 18758450-Fig-5d

chemicals

  *** Tak1[phosAb] phos(T187)[phosAb] is increased irt Tgfb1 (times)
    *** cells: mMesangialCells in BMLS
    *** times: 0 2+ 5++ 15+ min
    *** unaffected by: SB431542 [pretreatment]
    *** source: 19556242-Fig-3b

no extras

  *** Tak1[phosAb] phos(T187/S192)[phosAb] is increased irt Tgfb1 (5 min)
    *** cells: mEFs<xTraf6> in BMS
    *** source: 18758450-Fig-5b
  *** Tak1[phosAb] phos(T187/S192)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HEK293T in BMS
    *** times: 0+ 2++ 5++ 15+ min
    *** source: 18758450-Fig-S1f
  *** xTak1[phosAb] phos(T187/S192)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HEK293T<xTak1><xUbiq> in BMS
    *** times: 0+ 2++ 5++ 15++ min
    *** source: 18758450-Fig-1a
  *** Tak1[phosAb] phos(T187/S192)[phosAb] is increased irt Tgfb1 (times)
    *** cells: mEFs in BMS
    *** times: 0+ 2++ 5++ 15++ min
    *** source: 18758450-Fig-5a


