===============
Kinase Activity
===============

  *** xRaf1[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xNras(G12V)"CA"
    *** cells: HEK293T in BMS
    *** unaffected by: xShoc2 [addition]
    *** source: 16630891-Fig-2b,4a
  *** xRaf1[tAb]IP IVKA(Mek?,Erk2)[32P-ATP] is increased itpo xNras(G12V)"CA"
    *** cells: HEK293T in BMS
    *** source: 15143186-Fig-1a
  *** xRaf1[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xNras(G12V)"CA"
    *** cells: COS1 in BMLS "containing mevalonolactone"
    *** comment: both Kras-1 and Kras-2 splice variants were measured
    *** source: 10358073-Fig-1a,b

===================
Protein Interaction
===================

Direct Interaction between Raf1 and Nras-GTP

  *** rRaf1[tAb] boundby[WB] rNras{GTPgS}[GST] is detectable
    *** cells: none
    *** inhibited by: rNras{GDPbS} [substitution]
    *** source: 9674433-Fig-5b


does not req Shoc2--

  *** Raf1[Ab] copptby[WB] xNras[tAb] is detectable
    *** cells: HEK293T in BMS
    *** does not req: Shoc2 [RNAi]
    *** source: 16630891-Fig-1b,1f


  *** xRaf1[tAb] copptby[WB] xNras(G12V)"CA"[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 15143186-Fig-1e
  *** Raf1[Ab] copptby[WB] xNras(G12V)[GST] is detectable
    *** cells: HEK293T in BMS
    *** does not req: Shoc2 [RNAi]
    *** source: 16630891-Fig-1b,1f
  *** xRaf1[tAb] copptby[WB] xNras(G12V)[GST] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xShoc2 [addition]
    *** partially inhibited by: xMras(Q81L)"CA" [addition]
    *** source: 16630891-Fig-3b,6b



  --- phos state

  *** xRaf1[phosAb] copptby[WB] xNras(G12V)[GST] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xShoc2 [addition]
    *** inhibited by: xMras(H81L)"CA" [addition]
    *** comment: Raf1 is totally dephosed by (xShoc2 + xMras(H81L)"CA") [addition]
    *** comment: Coppt Raf1 is phosed on S259[phosAb]
    *** source: 16630891-Fig-6b

