====================
acetyl on K120
====================

  *** rTp53(K120R/K370R/K372R/K373R/K381R/K382R/K386R)"7KR" acetyl(K164)[AcAb] is increased by rEp300
    *** cells: none
    *** inhibited by: rTp53(K120R/K164R/K370R/K372R/K373R/K381R/K382R/K386R)"8KR" [substitution]
    *** source: 18485870-Fig-1d

====================
acetyl on K373
====================

  *** rTp53[AcAb] acetyl(K373)[AcAb] is increased by rEp300(1195-1810)
    *** cells: none
    *** source: 9891054-Fig-7b
  *** rTp53[AcAb] acetyl(K373)[AcAb] is increased by rEp300(1195-1810)
    *** cells: none
    *** unaffected by: xTp53(K319R/K320R/K321R) [substitution]
    *** inhibited by: xTp53(K373R) [substitution]
    *** source: 9891054-Fig-7a
  *** rTp53[Ab] acetyl(K373)[AcAb] is increased by rEp300
    *** cells: none
    *** comment: unaffected by previous ubiq by (rE4f1 + rUbe2d2 + rUbe1 + rUbiq)
    *** source: 17110336-Fig-S5a

requires Pin1 binding sites

  *** xTp53[AcAb] acetyl(K373)[AcAb] is increased itpo xEp300
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** bkg inhibited by: xTp53(S33A/S46A/T81A/S315A) [substitution]
    *** source: 17906639-Fig-S4a

====================
acetyl on K382
====================

  *** rTp53[KAcAb] acetyl(K382)[AcAb] is increased by rEp300
    *** cells: none
    *** source: 9744860-Fig-6
  *** xTp53[AcAb] acetyl(K382)[AcAb] is increased itpo xEp300
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** comment: enhanced by cotransfection of pG13 (plasmid containing Tp53 binding sites)
    *** comment: unaffected by cotransfection of pM13 (plasmid containing mutated Tp53 binding sites)
    *** source: 17906639-Fig-6a
  *** xTp53[Ab] acetyl(K382)[AcAb] is increased itpo xEp300
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** source: 17906639-Fig-S8a
  *** xTp53[Ab] acetyl(K382)[AcAb] is increased itpo xEp300
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** unaffected by: xPpp1r13l [addition]
    *** source: 17906639-Fig-S8b

requires Pin1 binding sites

  *** xTp53[AcAb] acetyl(K382)[AcAb] is increased itpo xEp300
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** partially inhibited by: xTp53(T81A) [substitution]
    *** partially inhibited by: xTp53(P81I) [substitution]
    *** source: 17906639-Fig-6a
  *** xTp53[AcAb] acetyl(K382)[AcAb] is increased itpo xEp300
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** inhibited by: xTp53(S33A/S46A/T81A/S315A) [substitution] no-bkg-control
    *** source: 17906639-Fig-S4a

======================
No acetyl on K320
======================

  *** rTp53[AcAb] acetyl(K320)[AcAb] is unchanged by rEp300(1195-1810)
    *** cells: none
    *** source: 9891054-Fig-7a
  *** rTp53[AcAb] acetyl(K320)[AcAb] is unchanged by rEp300(1195-1810)
    *** cells: none
    *** source: 9891054-Fig-7b

=================
Total acetyl
=================

  *** sTp53(319-393)[14C] acetyl[14C-acetyl-CoA] is increased by rEp300
    *** cells: none
    *** unaffected by: sTp53(319-393/pS392) [substitution]
    *** unaffected by: sTp53(319-393/pS378) [substitution]
    *** partially inhibited by: sTp53((319-393)/L323A/Y327A/L330A) [substitution]
    *** source: 9744860-Fig-4c
  *** rTp53(319-393)[MS] acetyl(K382)[MS] is increased by rEp300
    *** cells: none
    *** source: 9744860-Fig-3a
  *** rTp53[14C] acetyl[14C-acetyl-CoA] is increased by rEp300
    *** cells: none
    *** inhibited by: rTp53(1-335) [substitution]
    *** unaffected by: rTp53(283-393) [substitution]
    *** unaffected by: rTp53(318-393) [substitution]
    *** source: 9744860-Fig-1c
  *** sTp53[14C] acetyl[14C-acetyl-CoA] is increased by rEp300
    *** cells: none
    *** partially inhibited by: sTp53(1-57) [addition]
    *** inhibited by: sTp53(1-39) [addition]
    *** inhibited by: sTp53(1-39/pS9) [addition]
    *** inhibited by: sTp53(1-39/pS15) [addition]
    *** partially inhibited by: sTp53(1-39/pS20) [addition]
    *** partially inhibited by: sTp53(1-39/pS33) [addition]
    *** partially inhibited by: sTp53(1-39/pS37) [addition]
    *** source: 9744860-Fig-9
  *** rTp53[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(mnr)"HAT-domain"
    *** cells: none
    *** source: 17478422-Fig-1b
  *** rTp53[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(1195-1810)
    *** cells: none
    *** source: 9288740-Fig-1a
  *** rTp53[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(1195-1810)
    *** cells: none
    *** source: 9288740-Fig-1b
  *** rTp53[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(1195-1810)
    *** cells: none
    *** inhibited by: rTp53(1-73) [substitution]
    *** inhibited by: rTp53(100-290) [substitution]
    *** unaffected by: rTp53(290-393) [substitution]
    *** inhibited by: rTp53(284-330) [substitution]
    *** inhibited by: rTp53(1328-368) [substitution]
    *** unaffected by: rTp53(364-393) [substitution]
    *** unaffected by: rTp53(290-393) [substitution]
    *** source: 9288740-Fig-2b-c
  *** xTp53[Ab]IP acetyl[3H-acetate] is detectable
    *** cells: SAOS2 in BMS
    *** inhibited by: xTp53(1-369) [substitution]
    *** source: 9288740-Fig-3b
  *** rTp53(300-393)[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(1195-1810)
    *** cells: none
    *** inhibited by: rTp53(300-393/K373R) [substitution]
    *** unaffected by: rTp53(300-393/K381R) [substitution]
    *** unaffected by: rTp53(300-393/K382R) [substitution]
    *** source: 9891054-Fig-4a
  *** rTp53[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(1195-1810)
    *** cells: none
    *** inhibited by: rTp53(1-368) [substitution]
    *** source: 9891054-Fig-1
  *** rTp53[14C] acetyl[14C-acetyl-CoA] is increased by rEp300(1195-1810)
    *** cells: none
    *** source: 9891054-Fig-7a
  *** rTp53[14C] acetyl[14C-acetyl-CoA] is increased by xEp300[tAb]IP
    *** cells: none
    *** source: 16135789-Fig-1a

================================================
Requirement for Pin1 for Association with Ep300
================================================

  *** rTp53[AcAb] acetyl(K382)[AcAb] is increased by rEp300
    *** cells: none
    *** enhanced by: rPin1 [pretreatment]
    *** unaffected by: rPin1(C113A)"CIA" [pretreatment]
    *** source: 17906639-Fig-4e

  *** Tp53[Ab] copptby[WB] Ep300[Ab] is detectable
    *** cells: HCT116 in BMS
    *** reqs: Pin1 [RNAi]
    *** source: 17906639-Fig-4c

requires Pin1 binding sites

  *** xTp53[Ab] copptby[WB] xEp300[tAb] is detectable
    *** cells: SAOS2<Tp53~null><xTp53><xEp300> in BMS
    *** partially inhibited by: xTp53(S33A/S46A/T81A/S315A) [substitution]
    *** inhibited by: xTp53(S33A/S46A/T81A/S127A/T150A/S315A) [substitution]
    *** source: 17906639-Fig-4a
