==========
Activation
==========

IqGap1--

  *** Braf[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Pdgf (10 min)
    *** cells: mEFs in BMLS
    *** reqs: IqGap1 [KO]
    *** reqs: IqGap1 [RNAi]
    *** source: 17563371-Fig-3e

Pin1--

  *** Braf[Ab]IP IVKA(Mek?)[32P-ATP] is increased irt Pdgf (5 min)
    *** cells: mEFs in BMLS
    *** reqs: Pin1 [KO]
    *** source: 19933846-Fig-1g

chemicals

  *** Braf[Ab]IP IVKA(auto)[32P-ATP] is increased irt Pdgf (5 min)
    *** cells: PC12<xPdgfRb> in BMLS
    *** inhibited by: Forskolin [addition]
    *** source: 7935374-Fig-3a,3b,4a,4b,4c
  *** Braf[Ab]IP IVKA(Mek1,Erk2)[32P-ATP] is increased irt Pdgf (5 min)
    *** cells: PC12<xPdgfRb> in BMLS
    *** inhibited by: Forskolin [addition]
    *** source: 7935374-Fig-5a,5b,6a,6b
  *** Braf[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Pdgf (5 min)
    *** cells: PC12<xPdgfRb> in BMLS
    *** bkg inhibited by: Forskolin [addition]
    *** source: 7935374-Fig-2a,2b

===============
Phosphorylation
===============

  *** xBraf[phosAb] phos(S446)[phosAb] is unchanged irt Pdgf (15 min)
    *** cells: HEK293 in BMLS
    *** source: 16803888-Fig-3b
  *** Braf[Ab] phos[WBMS] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 30+ 240 min
    *** inhibited by: U0126 [pretreatment]
    *** source: 19933846-Fig-1a
  *** Braf[Ab]IP phos[32Pi] is increased irt Pdgf (30 min)
    *** cells: NIH3T3 in BMLS
    *** comment: phos sites were shown to be S151 T401 S750 T753 by HPLC
    *** inhibited by: U0126 [pretreatment]
    *** source: 19933846-Fig-2a
  *** Braf[Ab]IP STphos[pSTAb] is increased irt Pdgf (30 min)
    *** cells: NIH3T3 in BMLS
    *** inhibited by: U0126 [pretreatment]
    *** source: 19933846-Fig-1d
  *** Braf[Ab]IP STphos[pSTAb] is increased irt Pdgf (30 min)
    *** cells: mEFs in BMLS
    *** does not req: Pin1 [KO]
    *** source: 19933846-Fig-1f

========
Location
========

  *** Braf[Ab] locatedin(cellmembrane)[IHC] is increased irt Pdgf (30 min)
    *** cells: NIH3T3 in BMLS
    *** source: 19933846-Fig-1e

====================
Protein Interactions
====================

Hras--

  *** xBraf[tAb] snaggedby[WB] rHras(G12V)[GST] is decreased irt Pdgf (30 min)
    *** cells: NIH3T3<xBraf> in BMLS
    *** reversed by: xBraf(S151A/S401A/S750A/T753A) [substitution]
    *** source: 19933846-Fig-3a

Pin1--

  *** Braf[Ab] snaggedby[WB] rPin1[GST] is increased irt Pdgf (30 min)
    *** cells: NIH3T3 in BMLS
    *** source: 19933846-Fig-1b

Raf1--

  *** Braf[Ab] copptby[WB] Raf1[Ab] is increased irt Pdgf (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 5+ 30 min
    *** comment: coppt prolonged to 30 min irt U0126 pretreament
    *** source: 19933846-Fig-3b
  *** Braf[Ab] copptby[WB] xRaf1[tAb] is increased irt Pdgf (5 min)
    *** cells: NIH3T3<xRaf1> in BMLS
    *** inhibited by: xRaf1(S621A) [substitution]
    *** source: 19933846-Fig-3e


