======================
Direct Phosphorylation
======================

  *** rMkk4(K131M)[32P] phos[32P-ATP] is increased by rMekk1
    *** cells: none
    *** unaffected by: rPak1 [addition]
    *** unaffected by: Okadaic-acid [addition]
    *** source: 12228228-Fig-7c

==========================
SemiDirect Phosphorylation
==========================

  *** rMkk4[32P] phos[32P-ATP] is increased by xMekk1(352-1514)[tAb]IP
    *** cells: none
    *** comment: xMekk1(352-1514)"CA"[tAb]IP from COS1 in BMS
    *** inhibited by: xMekk1((352-1514)/K432M)"KD"[tAb]IP [substitution]
    *** source: 9819420-Fig-5b


======================
semi-direct activation
======================

  *** rMkk4[GST] IVKA(Jnk?,Jun)[32P-ATP] is increased by xMekk1(Cterm)[tAb]IP
    *** cells: COS in BMS
    *** comment: xMekk1[tAb]IP was from COS cells
    *** source: 8702571-Fig-3a

==========
activation
==========

  *** xMkk4[tAb]IP IVKA(Jnk1,Jun)[32P-ATP] is increased itpo xMekk1
    *** cells: COS7 in BMS
    *** source: 9891090-Fig-7
  *** xMkk4[tAb]IP IVKA(P38?)[32P-ATP] is increased itpo xMekk1(CatDom)
    *** cells: COS1 in BMS
    *** source: 9808624-Fig-2a
  *** xMkk4[tAb]IP IVKA(Jnk1)[32P-ATP] is increased itpo xMekk1
    *** cells: NIH3T3 in BMLS
    *** source: 9006902-Fig-3a
  *** Mkk4[Ab]IP IVKA(Jnk1,Atf2)[32P-ATP] is increased itpo xMekk1
    *** cells: COS1 in BMS
    *** source: 9639566-Fig-1a

===============
phosphorylation
===============

  *** xMkk4[tAb]IP phos[32Pi] is increased itpo xMekk1
    *** cells: HELA in BMS
    *** source: 9003778-Fig-1b

====================
physical interaction
====================

  *** xMekk1[tAb] snaggedby[WB] rMkk4[GST] is detectable
    *** cells: COS1 in BMS
    *** unaffected by: xMekk1(mnr)"KD" [substitution]
    *** source: 9808624-Fig-4a

