====================
Recruitment to Gp130
====================

  *** Stat1[Ab] copptby[WB] Gp130[Ab] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** comment: coppt is reversed when DTT is added to the ppt buffer
    *** source: 8272872-Fig-4a

===========================
Phosphorylation on Tyrosine
===========================

reqs Jak1--

  *** Stat1[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: U4A<Jak1~null><xJak1> in BMS
    *** reqs: xJak1 [omission]
    *** source: 7537214-Fig-5d

Jak2--  ???

  *** Stat1[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: 2C4 in BMS
    *** inhibited by: xJak2(mnr)"KD" [addition]
    *** source: 7537214-Fig-7c
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: GAMMA2A<Jak2~null> in BMS
    *** source: 7537214-Fig-5d
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: GAMMA2A<Jak2~null><xJak2> in BMS
    *** does not req: xJak2 [omission]
    *** inhibited by: xJak2(mnr)"KD" [substitution]
    *** source: 7537214-Fig-7a

does not req Tyk2--

  *** Stat1[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: U1D<Tyk2~null> in BMS
    *** source: 7537214-Fig-5d

Chemicals

  *** Stat1[Ab]IP Yphos[pYAb] is increased irt IL6 (20 min)
    *** cells: HEPG2 in BMS
    *** inhibited by: Genistein [pretreatment]
    *** inhibited by: Staurosporine [pretreatment]
    *** source: 8164674-Fig-10

No extras

  *** Stat1[Ab]IP Yphos[pYAb] is increased irt IL6 (20 min)
    *** cells: HEPG2 in BMS
    *** source: 8272872-Fig-1
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: 2C4 in BMS
    *** source: 7537214-Fig-7a
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: 2FTGH in BMS
    *** source: 7537214-Fig-5d
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMS
    *** source: 8272872-Fig-2c
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt IL6 (times)
    *** cells: HEPG2 in BMS
    *** times: 0 2+ 5++ 15+++ 30+ 60 min
    *** source: 8164674-Fig-9a

=======================
Phosphorylation on Y701
=======================

  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt IL6 (15 min)
    *** cells: HEPG2 in BMS
    *** source: 9724754-Fig-4c
  *** Stat1[phosAb] phos(Y701)[phosAb] is unchanged irt IL6 (20 min)
    *** cells: U4A<Jak1~null><xJak1> in BMS
    *** source: 15935617-Fig-3b
