=======================================================================
Ikk2 is phosphorylated by free Ubiq chains produced by Ube2d3 and Traf6
=======================================================================

  *** pIkk2[phosAb] phos(SLCTS)[phosAb] is increased by rUbiq{poly2}
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rUbiq was polymerized with (pUbe1 + rUbe2d3 + rTraf6)
    *** comment: inhibited by short linear Ubiq chains [addition]
    *** comment: inhibited by short K63-linked Ubiq chains [addition]
    *** source: 19675569-Fig-S9c
  *** pIkk2[phosAb] phos(SLCTS)[phosAb] is increased by rTraf6{ubiq}
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rTraf6 was ubiquitinated by (rUbiq + pUbe1 + rUbe2d3)
    *** inhibited by: rUsp5 [addition]
    *** unaffected by: rUsp5(C335A) [addition]
    *** source: 19675569-Fig-4c
  *** pIkk2[phosAb] phos(SLCTS)[phosAb] is increased by rTraf6{ubiq}
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rTraf6 was ubiquitinated with (rUbiq + pUbe1 + rUbe2d3) followed by inactivation of E1 and E2 with NEM
    *** inhibited by: rUbiq(dig)"K48-only" [substitution]
    *** unaffected by: rUbiq(K48R) [substitution]
    *** inhibited by: rUbiq(K63only) [substitution]
    *** unaffected by: rUbiq(K63R) [substitution]
    *** inhibited by: rUbiq(dig)"no-lysines" [substitution]
    *** source: 19675569-Fig-S8b
  *** pIkk2[phosAb] phos(SLCTS)[phosAb] is increased by rTraf6{ubiq}
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rTraf6 was ubiquitinated with (rUbiq + pUbe1 + rUbe2d3) followed by inactivation of E1 and E2 with NEM
    *** source: 19675569-Fig-S6b

=================================================
Ikk2 phosphorylation by Ubiq-poly2 requires Nemo
=================================================

  *** pIkk2[phosAb] phos(SLCTS)[phosAb] is increased by (rTraf6{ubiq} + rNemo)
    *** cells: none
    *** comment: Ikks were purified from 13E2<Nemo~null> cells by HiTrapQ fractionation
    *** comment: rTraf6 was ubiquitinated with (rUbiq + pUbe1 + rUbe2d3) followed by inactivation of E1 and E2 with NEM
    *** reqs: rNemo [omission]
    *** inhibited by: rNemo(Y308S)"Ubiq-binding-mutant" [substitution]
    *** source: 19675569-Fig-S7b

==================================================================
Ikk2 is activated by free Ubiq chains produced by Ube2d3 and Traf6
==================================================================

  *** rIkba[Ab] phos[WBMS] is increased by (rTraf6{ubiq} + pIkks + rNemo)
    *** cells: none
    *** comment: Ikks were purified from 13E2<Nemo~null> cells by HiTrapQ fractionation
    *** comment: rTraf6 was ubiquitinated with (rUbiq + pUbe1 + rUbe2d3) followed by inactivation of E1 and E2 with NEM
    *** reqs: rNemo [omission]
    *** inhibited by: rNemo(Y308S)"Ubiq-binding-mutant" [substitution]
    *** source: 19675569-Fig-S7b
  *** rIkba[Ab] phos[WBMS] is increased by (rTraf6{ubiq} + pIkks)
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rTraf6 was ubiquitinated by (rUbiq + pUbe1 + rUbe2d3)
    *** inhibited by: rUsp5 [addition]
    *** unaffected by: rUsp5(C335A) [addition]
    *** source: 19675569-Fig-4c
  *** rIkba[Ab] phos[WBMS] is increased by (rTraf6{ubiq} + pIkks)
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rTraf6 was ubiquitinated with (rUbiq + pUbe1 + rUbe2d3) followed by inactivation of E1 and E2 with NEM
    *** inhibited by: rUbiq(dig)"K48-only" [substitution]
    *** unaffected by: rUbiq(K48R) [substitution]
    *** inhibited by: rUbiq(K63only) [substitution]
    *** unaffected by: rUbiq(K63R) [substitution]
    *** inhibited by: rUbiq(dig)"no-lysines" [substitution]
    *** source: 19675569-Fig-S8b
  *** rIkba[Ab] phos[WBMS] is increased by (rTraf6{ubiq} + pIkks)
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rTraf6 was ubiquitinated with (rUbiq + pUbe1 + rUbe2d3) followed by inactivation of E1 and E2 with NEM
    *** source: 19675569-Fig-S6b

  *** rIkba[Ab] phos[WBMS] is increased by (rUbiq{poly2} + pIkks)
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rUbiq was polymerized with (pUbe1 + rUbe2d3 + rTraf6)
    *** source: 19675569-Fig-S10c
  *** rIkba[Ab] phos[WBMS] is unchanged by (rUbiq{poly1} + pIkks)
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rUbiq was polymerized with (pUbe1 + (rUbc13 + rUev1a) + rTraf6)
    *** source: 19675569-Fig-S10c

===========================================================================================
Ikk2 is not phosphorylated/activated by free ubiq chains produced by Ubc13/Uev1a and Traf6
===========================================================================================

  *** pIkk2[phosAb] phos(SLCTS)[phosAb] is unchanged by rUbiq{poly1}
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rUbiq was polymerized with (pUbe1 + (rUbc13 + rUev1a) + rTraf6) followed by inactivation of E1 and E2 with NEM
    *** source: 19675569-Fig-S6b
  *** rIkba[Ab] phos[WBMS] is unchanged by (rUbiq{poly1} + pIkks)
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rUbiq was polymerized with (pUbe1 + (rUbc13 + rUev1a) + rTraf6) followed by inactivation of E1 and E2 with NEM
    *** source: 19675569-Fig-S6b

===========================================================================================
Ikk2 is not phosphorylated/activated by free ubiq chains produced by Ube2d3 and Rnf31/Rbck1
===========================================================================================

  *** rIkba[Ab] phos[WBMS] is unchanged by (rUbiq{poly3} + pIkks)
    *** cells: none
    *** comment: Ikks were purified from HELA cells by HiTrapQ fractionation
    *** comment: rUbiq was polymerized with (pUbe1 + rUbe2d3 + (rRnf31 + rRbck1))
    *** source: 19675569-Fig-S10c
