===============
Kinase Activity
===============

not Msk2--

  *** Msk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt PMA (10 min)
    *** cells: mEFs in BMLS
    *** reqs: Msk1 [KO]
    *** does not req: Msk2 [KO]
    *** reqs: Msk1 and/or Msk2 [DKO]
    *** source: 11909979-Fig-4b

not Pdpk1--

  *** Msk1[Ab]IP IVKA(Creb1)[32P-ATP] is increased irt PMA (15 min)
    *** cells: mESCs in BMLS
    *** does not req: Pdpk1 [KO]
    *** source: 10801415-Fig-6a

Chemicals

  *** Msk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt PMA (10 min)
    *** cells: mEFs in BMLS
    *** inhibited by: PD184352 [addition] no-bkg-control
    *** unaffected by: SB203580 [addition] no-bkg-control
    *** source: 11909979-Fig-3b
  *** Msk1[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** unaffected by: SB203580 [pretreatment]
    *** source: 9687510-Fig-5a
  *** Msk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt PMA (30 min)
    *** cells: C3H10T12 in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** unaffected by: H89 [pretreatment]
    *** source: 10469656-Fig-9a
  *** Msk1[Ab]IP IVKA(Creb1)[32P-ATP] is increased irt PMA (10 min)
    *** cells: mESCs in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** source: 11018520-Fig-2b

required features

  *** xMsk1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** partially inhibited by: xMsk1(S360A) [substitution]
    *** inhibited by: xMsk1(S376A) [substitution]
    *** inhibited by: xMsk1(T581A) [substitution]
    *** inhibited by: xMsk1(S212A) [substitution]
    *** unaffected by: xMsk1(S381A) [substitution]
    *** partially inhibited by: xMsk1(L740A)"Mapk-docking-mut" [substitution]
    *** inhibited by: xMsk1(R743A/R744A)"Mapk-docking-mut" [substitution]
    *** unaffected by: xMsk1(S750A/S752A) [substitution]
    *** unaffected by: xMsk1(S758A) [substitution]
    *** source: 15568999-Fig-3,4a,6,7
  *** xMsk1[tAb]IP IVKA(Crosstide)[32P-ATP] is increased irt PMA (10 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** unaffected by: SB203580 [pretreatment]
    *** inhibited by: xMsk1(D195A)"NT-KD" [substitution]
    *** inhibited by: xMsk1(D565A)"CT-KD" [substitution]
    *** source: 9687510-Fig-6a

no extras

  *** xMsk1[GST]IP IVKA(Crosstide)[32P-ATP] is increased irt PMA (15 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** source: 9687510-Fig-3a
  *** Msk1[Ab]IP IVKA(Creb1)[32P-ATP] is increased irt PMA (times)
    *** cells: mESCs in BMLS
    *** times: 0 10+++ 20++ 30+ min
    *** source: 11018520-Fig-3a

===============
Phosphorylation
===============

phos(S212)

  *** Msk1[Ab]IP phos(S212)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(S212)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: PD184352 [addition]
    *** unaffected by: SB203580 [addition]
    *** source: 15568999-Fig-2a

phos(S360)

  *** Msk1[Ab]IP phos(S360)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(S360)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: PD184352 [addition]
    *** unaffected by: SB203580 [addition]
    *** source: 15568999-Fig-2a

phos(S376)

  *** Msk1[Ab]IP phos(S376)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(S376)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: PD184352 [addition]
    *** unaffected by: SB203580 [addition]
    *** source: 15568999-Fig-2a

phos(S381)

  *** Msk1[Ab]IP phos(S381)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(S381)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: PD184352 [addition]
    *** unaffected by: SB203580 [addition]
    *** source: 15568999-Fig-2a

phos(T581)

  *** Msk1[Ab]IP phos(T581)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(T581)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: PD184352 [addition]
    *** unaffected by: SB203580 [addition]
    *** source: 15568999-Fig-2a

phos(S750)

  *** Msk1[Ab]IP phos(S750)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** source: 15568999-Fig-2b
  *** xMsk1[phosAb] phos(S750)[phosAb] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: PD184352 [addition]
    *** unaffected by: SB203580 [addition]
    *** source: 15568999-Fig-2a

unknown site

  *** xMsk1[GST]IP phos[MS] is increased irt PMA (15 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** source: 9687510-Fig-3a

========
Location
========

  *** xMsk1[tAb] locatedin(nucleus)[EM] is detectable-but unchanged irt PMA (10 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** source: 9687510-Fig-7a

