=================
Required Proteins
=================

not Fadd--

  *** IL6-gene mRNA[RTPCR] is increased irt IL1b (4 hr)
    *** cells: mEFs in BMS
    *** does not req: Fadd [KO]
    *** source: 16585540-Fig-1a

Ikk1--

  *** IL6-gene mRNA[Northern] is increased irt IL1b (4 hr)
    *** cells: mEFs in BMS
    *** reqs: Ikk1 [KO]
    *** source: 11733537-Fig-1a

Ikk2--

  *** IL6-gene mRNA[Northern] is increased irt IL1b (4 hr)
    *** cells: mEFs in BMS
    *** reqs: Ikk2 [KO]
    *** source: 11733537-Fig-1a

not Ikke--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs in BMS
    *** repressed by: Ikke [KO]
    *** source: 15210742-Fig-2a

Irak1--

  *** IL6-gene mRNA[Northern] is increased irt IL1b (3 hr)
    *** cells: mEFs in BMLS
    *** reqs: Irak1 [KO]
    *** source: 9625767-Fig-4a
  -----------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** partially reqs: Irak1 [RNAi]
    *** source: 19181383-Fig-2a
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (8 hr)
    *** cells: mEFs in BMLS
    *** reqs: Irak1 [KO]
    *** source: 9625767-Fig-4b
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (8 hr)
    *** cells: mSFs in BMLS "in suspension"
    *** reqs: Irak1 [KO]
    *** source: 9625767-Fig-4b

not Irak3--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mBMMs in BMS
    *** repressed by: Irak3 [KO]
    *** source: 12150927-Fig-1e

Irak4--

  *** IL6-gene mRNA[RTPCR] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** does not req: Irak1 [RNAi]
    *** source: 19181383-Fig-3b
  *** IL6-gene mRNA[RTPCR] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** reqs: Irak1 and/or Irak4 [RNAi]
    *** source: 19181383-Fig-3b
  *** IL6-gene mRNA[RTPCR] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** reqs: Irak4 [RNAi]
    *** source: 19181383-Fig-3a
  *** IL6-gene mRNA[RTPCR] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 1+ 2++ 4+++ 6++ hr
    *** inhibited by: xIrak4(K213A/K214A)"KD" [knockin]
    *** source: 17337443-Fig-5b
  *** IL6-gene promo-reporter[Luc] is increased irt IL1b (8 hr)
    *** cells: mEFs in BMS
    *** inhibited by: xIrak4(K213A/K214A)"KD" [knockin]
    *** source: 17337443-Fig-4a
  ---------------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (18 hr)
    *** cells: hUVECs in BMS
    *** reqs: Irak4 [RNAi]
    *** source: 19181383-Fig-2a
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mBMMs in BMS
    *** inhibited by: kIrak4(K213A/K214A)"KD" [knockin]
    *** source: 17470642-Fig-3b
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs in BMS
    *** reqs: Irak4 [KO]
    *** source: 15292196-Fig-1a
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs in BMS
    *** reqs: Irak4 [KO]
    *** source: 15292196-Fig-4a
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs<Irak4~null><xIrak4> in BMS
    *** reqs: xIrak4 [omission]
    *** inhibited by: xIrak4(mnr)"KD" [substitution]
    *** source: 15292196-Fig-4a
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs<Irak4~null><xIrak4> in BMS
    *** reqs: xIrak4 [omission]
    *** inhibited by: xIrak4(mnr)"KD" [substitution]
    *** source: 15292196-Fig-4b
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 16+ 24++ 40+++ hr
    *** inhibited by: xIrak4(K213A/K214A)"KD" [knockin]
    *** source: 17337443-Fig-6a
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (tnr)
    *** cells: mEFs in BMS
    *** reqs: Irak4 [KO]
    *** source: 11923871-Fig-2c

Jnk1-- and/or Jnk2--

  *** IL6-gene mRNA[RTPCR] is increased irt IL1a (times)
    *** cells: mEFs in BMLS
    *** times: 0 15+ 30++ 60+++ 120++++ 180++ 240+ min
    *** reqs: Jnk1 and/or Jnk2 [KO]
    *** source: 18443042-Fig-S1


Jun--  phos(S63A/S73A)

  *** IL6-gene mRNA[RTPCR] is increased irt IL1a (times)
    *** cells: mEFs in BMLS
    *** times: 0 15+ 30++ 60+++ 120++++ 180++ 240+ min
    *** reqs: Jun [KO]
    *** inhibited by: kJun(S63A/S73A) [substitution]
    *** source: 18443042-Fig-S1

Mekk3--

  *** IL6-gene mRNA[Northern] is detectable
    *** cells: mEFs in BMS "treated with IL1b (6 hr)"
    *** reqs: Mekk3 [KO]
    *** source: 14661019-Fig-1c
  *** IL6-gene mRNA[Northern] is detectable
    *** cells: mEFs<Mekk3~null><xMekk3> in BMS "treated with IL1b (6 hr)"
    *** source: 14661019-Fig-1c
  ----------------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 12+ 24+ hr
    *** reqs: Mekk3 [KO]
    *** source: 14661019-Fig-1a
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 12+ 24+ hr
    *** reqs: Mekk3 [KO]
    *** source: 14661019-Fig-1b
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 12+ 24+ hr
    *** does not req: Mekk2 [KO]
    *** source: 14661019-Fig-1b
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs in BMS
    *** reqs: Mekk3 [KO]
    *** source: 14661019-Fig-2b
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs<Mekk3~null><xMekk3> in BMS
    *** reqs: xMekk3 [omission]
    *** source: 14661019-Fig-2c
  *** IL6[Ab] secretion[ELISA] is unchanged irt IL1b (24 hr)
    *** cells: mEFs<Mekk3~null><xMekk2> in BMS
    *** source: 14661019-Fig-2c

Myd88--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1 (20 hr)
    *** cells: hSNFs in BMS
    *** inhibited by: xMyd88(del(1-53)/F56N)"DN" [addition]
    *** source: 14630816(D)
  *** IL6[Ab] secretion[ELISA] is increased irt IL1 (20 hr)
    *** cells: hUVECs in BMS
    *** inhibited by: xMyd88(del(1-53)/F56N)"DN" [addition]
    *** source: 14630816(D)

Nemo--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (12 hr)
    *** cells: mEFs<Nemo~null><xNemo> in BMS
    *** reqs: xNemo [omission]
    *** partially inhibited by: xNemo(K285R) [substitution]
    *** source: 19112497-Fig-6b

Nfkbiz--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs in BMS
    *** partially reqs: Nfkbiz [KO]
    *** source: 15241416-Fig-2e

not Peli1--

  *** IL6-gene mRNA[RTPCR] is increased irt IL1b (times)
    *** cells: mEFs in BMLS
    *** times: 0 2++ 4+ hr
    *** does not req: Peli1 [KO]
    *** source: 19734906-Fig-5a

Pkcz--

  *** IL6-gene mRNA[RTPCR] is increased irt IL1 (5 hr)
    *** cells: mEFs in BMS
    *** partially reqs: Pkcz [KO]
    *** source: 11684013-Fig-2e

Rela--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs<Rela~null><xRela> in BMS
    *** reqs: xRela [omission]
    *** partially inhibited by: xRela(S276A) [substitution]
    *** unaffected by: xRela(S529A) [substitution]
    *** unaffected by: xRela(S536A) [substitution]
    *** unaffected by: xRela(S529A/S536A) [substitution]
    *** source: 12559944-Fig-2b

not Tab2--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (16 hr)
    *** cells: mEFs in BMS
    *** does not req: Tab2 [KO]
    *** source: 12556483-Fig-3b

Tak1--

  *** IL6-gene mRNA[RTPCR] is increased irt IL1 (3 hr)
    *** cells: mEFs<Tak1~null><xTak1> in BMS
    *** reqs: xTak1 [omission]
    *** unaffected by: xTak1(K34R) [substitution]
    *** inhibited by: xTak1(K209R) [substitution]
    *** source: 19843958-Fig-2h
  *** IL6-gene mRNA[RTPCR] is increased irt IL1b (8 hr)
    *** cells: mEFs<Tak1~null><xTak1> in BMS
    *** reqs: xTak1 [omission]
    *** inhibited by: xTak1(K158R) [addition]
    *** source: 20038579-Fig-8b
  *** IL6-gene mRNA[RTPCR] is increased irt IL1b (8 hr)
    *** cells: mEFs<Tak1~null><xTak1> in BMS
    *** inhibited by: xTak1(T178A/T184A) [substitution]
    *** source: 18617512-Fig-5a
  ------------------------------------------------------------
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs in BMS
    *** reqs: Tak1 [KO]
    *** source: 16186825(D)
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (times)
    *** cells: mEFs<Tak1~null><xTak1> in BMS
    *** times: 0 8+ 12+ 24+ hr
    *** reqs: xTak1 [omission]
    *** inhibited by: xTak1(K158R) [addition]
    *** source: 20038579-Fig-8d
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (8 hr)
    *** cells: mEFs<Tak1~null><xTak1> in BMS
    *** inhibited by: xTak1(T178A/T184A) [substitution]
    *** source: 18617512-Fig-5b

not Tbk1--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs in BMS
    *** does not req: Tbk1 [KO]
    *** source: 15210742-Fig-2a

not Trif--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs in BMS
    *** does not req: Ticam2 [KO]
    *** source: 14556004(D)

not Tirap--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1 (20 hr)
    *** cells: hSNFs in BMS
    *** unaffected by: xTirap(P125H)"DN" [addition]
    *** source: 14630816(D)
  *** IL6[Ab] secretion[ELISA] is increased irt IL1 (20 hr)
    *** cells: hUVECs in BMS
    *** unaffected by: xTirap(P125H)"DN" [addition]
    *** source: 14630816(D)

Traf6--

  *** IL6[Ab] secretion[ELISA] is increased irt IL1 (times)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** times: 0 12+ 24++ hr
    *** partially inhibited by: xTraf6(C242A/C249A)"T6mZ5" [substitution]
    *** inhibited by: xTraf6(258-522)"T6dRZ" [substitution]
    *** inhibited by: xTraf6(110-522)"T6dR" [substitution]
    *** source: 19843958-Fig-5f
  *** IL6[Ab] mRNA[RTPCR] is increased irt IL1 (times)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** times: 0+ 1++ 3+ hr
    *** partially inhibited by: xTraf6(C242A/C249A)"T6mZ5" [substitution]
    *** source: 19843958-Fig-5e
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (12 hr)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** reqs: xTraf6 [omission]
    *** bkg inhibited by: xTraf6(C70A) [substitution]
    *** unaffected by: xTraf6(mnr)"K32-518R" [substitution]
    *** source: 19112497-Fig-3f

no extras

  *** IL6-gene mRNA[RTPCR] is increased irt IL1b (times)
    *** cells: 3T3L1 in BMS "differentiated into adipocytes"
    *** times: 0+ 2++++ 6+++ 12++ 24++ 48++ hr
    *** source: 17500068-Fig-3c
  *** IL6[Ab] secretion[ELISA] is increased irt IL1b (24 hr)
    *** cells: mEFs in BMS
    *** source: 15241416-Fig-2g


===================
Inhibitory Proteins
===================

  *** IL6[Ab] secretion[ELISA] is increased irt IL1 (tnr)
    *** cells: THP1 in BMS
    *** repressed by: Rnf11 [RNAi]
    *** source: 19131965-Fig-3d




















