
does not req Rictor--

  *** Mlst8[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** does not req: Rictor [RNAi]
    *** source: 17510057-Fig-2a
  *** Mlst8[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: mEFs<Rictor~null> in BMS
    *** source: 18925875-Fig-4a

does not req Raptor--

  *** Mlst8[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** partially reqs: Raptor [RNAi]
    *** source: 17510057-Fig-2a
  *** xMlst8[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293T<xMtor><xMlst8><xPras40><xRaptor> in BMS
    *** does not req: xRaptor [omission]
    *** source: 17510057-Fig-2b

does not req Protor1--

  *** Mlst8[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HELA in BMS
    *** does not req: Protor1 [RNAi]
    *** source: 17461779-Fig-4c

Mlst8 features

  *** xMlst8[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xMlst8(F320S) [substitution]
    *** inhibited by: xMlst8(G192D) [substitution]
    *** inhibited by: xMlst8(S72D) [substitution]
    *** source: 12718876(D)

Chemicals

  *** Mlst8[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: Rapamycin [addition]
    *** unaffected by: AntimycinA [addition]
    *** source: 12718876(D)

Lysis Conditions

  *** Mlst8[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** comment: Coppt works in both Chaps-LB and TritonX-LB
    *** source: 15268862-Fig-2b

no extras

  *** xMlst8[tAb] copptby[WB] xMtor[tAb] is detectable
    *** cells: HEK293E<xMtor><xMlst8><xDeptor> in BMS
    *** source: 19446321-Fig-1f
  *** Mlst8[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17510057-Fig-1b
  *** Mlst8[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17277771-Fig-1b
  *** Mlst8[Ab] copptby[WB] Mtor[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 17461779-Fig-3a
