=======================
Phosphorylation on Y701
=======================

not Fadd--

  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mEFs in BMS
    *** times: 0 1++ 4+ hr
    *** does not req: Fadd [KO]
    *** source: 15549108-Fig-S3a

Jak1-- ???

  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (20 min)
    *** cells: U4A<Jak1~null><xJak1> in BMS
    *** inhibited by: xJak1(192-1125) [substitution]
    *** source: 12133952-Fig-4b

Jak2-- kinase activity

  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (15 min)
    *** cells: 2C4 in BMS
    *** inhibited by: xJak2(mnr)"KD" [addition]
    *** source: 7537214-Fig-7c
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (15 min)
    *** cells: GAMMA2A<Jak2~null><xJak2> in BMS
    *** reqs: xJak2 [omission]
    *** inhibited by: xJak2(mnr)"KD" [substitution]
    *** source: 7537214-Fig-7a

not Pias1--

  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mEFs in BMS
    *** times: 0 15++ 60+ 120+ min
    *** unaffected by: Pias1 [KO]
    *** source: 17606919-Fig-7b
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mSpCs in BMS
    *** times: 0 15+++ 60++ 120+ min
    *** unaffected by: Pias1 [KO]
    *** source: 15311277-Fig-S1

not Pias4--

  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mEFs in BMS
    *** times: 0 15+++ 60++ 120+ min
    *** unaffected by: Pias4 [KO]
    *** source: 17606919-Fig-7a


Stat1 features

  *** xStat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (20 min)
    *** cells: U3A<Stat1~null><xStat1> in BMS
    *** unaffected by: xStat1(N460A) [substitution]
    *** unaffected by: xStat1(K336A) [substitution]
    *** source: 11350940-Fig-6a
  *** xStat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (30 min)
    *** cells: U3A<Stat1~null><xStat1> in BMS
    *** unaffected by: xStat1(L407A) [substitution]
    *** source: 11927559-Fig-2a
  *** xStat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mEFs<Stat1~null> in BMS
    *** times: 0 30++ 120+ 360 720 min
    *** unaffected by: xStat1(K703R) [substitution]
    *** comment: xStat1(K703R) phos is extended to 360 min
    *** unaffected by: xStat1(E705A) [substitution]
    *** source: 16857984-Fig-3b
  *** xStat1(L407A)[phosAb] phos(Y701)[phosAb] is increased irt Ifng (30 min)
    *** cells: U3A<Stat1~null><xStat1> in BMS
    *** source: 11927559-Fig-3b
  *** xStat1[Ab]IP Yphos[pYAb] is increased irt Ifng (15 min)
    *** cells: U3A<Stat1~null> in BMS
    *** inhibited by: xStat1(Y701F) [substitution]
    *** source: 9724754-Fig-5a

chemicals

  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (30 min)
    *** cells: HELA in BMS
    *** unaffected by: N6-Methyl-2-deoxyadenosine [addition]
    *** source: 15153491-Fig-6b
  *** xStat1[Ab]IP Yphos[pYAb] is increased irt Ifng (times)
    *** cells: U3A<Stat1~null><xStat1> in BMS "treated with CHX"
    *** times: 0 30++ 60++ 90++ 120+ 240 480 min
    *** unaffected by: LMB [pretreatment]
    *** source: 11080165-Fig-2b

no extras

  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (15 min)
    *** cells: HEPG2 in BMS
    *** source: 9724754-Fig-4c
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (20 min)
    *** cells: 2C4 in BMS
    *** source: 11350940-Fig-2a
  *** xStat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (20 min)
    *** cells: 2C4<xStat1> in BMS
    *** source: 11350940-Fig-2a
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (20 min)
    *** cells: hKerats in BMS
    *** source: 15308104-Fig-4f
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (20 min)
    *** cells: hPBMCs in BMS
    *** source: 15308104-Fig-4f
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (30 min)
    *** cells: HEK293 in BMS
    *** source: 12070153-Fig-1b
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (30 min)
    *** cells: HEK293<xEgfR> in BMS
    *** source: 12070153-Fig-1b
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (30 min)
    *** cells: HELA in BMS
    *** source: 11927559-Fig-3b
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: A431 in BMS "nuclear fraction"
    *** times: 0 30++ 60++ 360++ 1440+ min
    *** source: 12270932-Fig-1a
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mAMCs in BMHIS
    *** times: 0 15+ 45+ min
    *** source: 15240691-Fig-1d
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mBMMs in BMS "nuclear-extracts"
    *** times: 0 30+ 60+ min
    *** source: 17293456-Fig-6
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mPECs in BMHIS
    *** times: 0 15+ 45+ min
    *** source: 15240691-Fig-1c
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mPECs in BMS
    *** times: 0 30+ 60+ 120+ 240+ min
    *** source: 11896392-Fig-3a
  *** Stat1[Ab]IP phos[32Pi] is increased irt Ifng (6 min)
    *** cells: HELA in BMS
    *** comment: most of the phos was on tyrosine by phosphoamino acid analysis
    *** source: 1496401-Fig-5
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: U4A<Jak1~null><xJak1> in BMS
    *** times: 0 15+ 30++ 60++ 90++ 120++ min
    *** source: 15935617-Fig-3a
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (20 min)
    *** cells: U4A<Jak1~null><xJak1> in BMS
    *** source: 15935617-Fig-3b
  *** Stat1[Ab]IP phos(Y701)[phosAb] is increased irt Ifng (30 min)
    *** cells: HEK293<xSumo1><xUbe2i> in BMS
    *** source: 16857984-Fig-1b
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (10 min)
    *** cells: 3112<xIfngR1> in BMS
    *** source: 7615558-Fig-3c
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (10 min)
    *** cells: HELA in BMS
    *** source: 7615558-Fig-11
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (10 min)
    *** cells: HEPG2 in BMS
    *** source: 8272872-Fig-2c
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (15 min)
    *** cells: 2C4 in BMS
    *** source: 7537214-Fig-7a
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (15 min)
    *** cells: HELA in BMS
    *** source: 7514165-Fig-4b
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (15 min)
    *** cells: HEPG2 in BMS
    *** source: 8140422-Fig-2c
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (20 min)
    *** cells: HEPG2 in BMS
    *** source: 8272872-Fig-1
  *** xStat1[Ab]IP Yphos[pYAb] is increased irt Ifng (30 min)
    *** cells: U3A<Stat1~null><xStat1> in BMS
    *** source: 11080165-Fig-1b
  *** Stat1[Ab]IP Yphos[pYAb] is increased irt Ifng (times)
    *** cells: HELA in BMS
    *** times: 0 1+ 2+ 5+ min
    *** source: 7514165-Fig-2c
  *** Stat1[Ab]IP Yphos[pYAb] is unchanged irt Ifng (10 min)
    *** cells: L929<xIfngR1> in BMS
    *** comment: L929 cells are mouse and do not respond to hIfng
    *** source: 7615558-Fig-3c
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mHepatocytes in BMLS
    *** times: 0 30+ 60+ 480 min
    *** source: 15246962-Fig-4
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mKupfferCells in BMLS
    *** times: 0 30++ 60+ 480 min
    *** source: 15246962-Fig-4
  *** Stat1[phosAb] phos(Y701)[phosAb] is increased irt Ifng (times)
    *** cells: mSinusoidalEndothelialCells in BMLS
    *** times: 0 30+ 60+ 480 min
    *** source: 15246962-Fig-4

=======================
Phosphorylation on S727
=======================

  *** Stat1[phosAb] phos(S727)[phosAb] is increased irt Ifng (times)
    *** cells: A431 in BMS "nuclear fraction"
    *** times: 0 30+ 60+ 360+ 1440+ min
    *** source: 12270932-Fig-1a
  *** xStat1[phosAb] phos(S727)[phosAb] is unchanged irt Ifng (30 min)
    *** cells: COS7<xSumo1> in BMS
    *** source: 17897103-Fig-1

=====================================
Phosphorylation on an unreported site
=====================================

  *** Stat1[phosAb] phos(sitenr)[phosAb] is increased irt Ifng (30 min)
    *** cells: mEFs in BMS
    *** does not req: Hmgb1 and/or Hmgb2 and/or Hmgb3 [RNAi]
    *** source: 19890330-Fig-S2m
  *** Stat1[phosAb] phos(sitenr)[phosAb] is increased irt Ifng (times)
    *** cells: mBMMs in BMS
    *** times: 0 25++ 100++ 400+ min
    *** does not req: Socs3 [KO]
    *** source: 12754506-Fig-1a
  *** Stat1[phosAb] phos(sitenr)[phosAb] is increased irt Ifng (times)
    *** cells: mBMMs<Ifng~null> in BMS
    *** times: 0 25++ 100++ 400+ min
    *** comment: repressed by Socs1 [KO] at 400 min only
    *** source: 12754506-Fig-1a
  *** Stat1[phosAb] phos(sitenr)[phosAb] is increased irt Ifng (times)
    *** cells: U2OS in BMS
    *** times: 0 10+ 20+ min
    *** does not req: Ubiq [RNAi]
    *** source: 19854138-Fig-S3a
  *** Stat1[phosAb] phos(sitenr)[phosAb] is increased irt Ifng (times)
    *** cells: U2OS<Ubiq~null><xUbiq> in BMS
    *** times: 0 10+ 20+ min
    *** enhanced by: xUbiq(K63R) [substitution]
    *** source: 19854138-Fig-S3f
  *** xStat1[phosAb] phos(sitenr)[phosAb] is increased irt Ifng (tnr)
    *** cells: U3A<Stat1~null> in BMLS
    *** unaffected by: xStat1(L407A/L409A) [substitution]
    *** source: 11823427-Fig-5d





