==================
Direct Neddylation
==================

  *** rTp53[Ab] upshift[WBMS] is increased by (rNae1 + rUba3 + rUbe2m + rNedd8)
    *** cells: none
    *** enhanced by: rMdm2 [addition]
    *** source: 15242646-Fig-3c
  *** rTp53[Ab] upshift[WBMS] is increased by (rNae1 + rUba3 + rUbe2m + rNedd8)
    *** cells: none
    *** source: 15242646-Fig-3a

  *** rTp53[35S] upshift[WBMS] is increased by (rMdm2 + rUbe2m + rNedd8)
    *** cells: none
    *** comment: not sure if ommision of E1 (rNae1 + rUba3) was a typo or not needed
    *** source: 17110336-Fig-3e


=========================
Neddylation requires Mdm2
=========================

  *** xTp53[Ab] nedd[tNdAb-ppt] is detectable
    *** cells: H1299<Tp53~null><xTp53><xNedd8> in BMS
    *** reqs: Mdm2 [RNAi]
    *** source: 15242646-Fig-2d
  *** xTp53[Ab] nedd[tNdAb-ppt] is detectable
    *** cells: MCF7<xTp53><xNedd8> in BMS
    *** reqs: Mdm2 [RNAi]
    *** source: 15242646-Fig-2d
  *** xTp53[Ab] nedd[tNdAb-ppt] is increased itpo xMdm2
    *** cells: CHO<xTp53><xNedd8> in BMS
    *** partially reqs: Nae1 [null-mut]
    *** source: 15242646-Fig-5a
  *** xTp53[Ab] nedd[tNdAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xNedd8> in BMS
    *** inhibited by: xSenp8 [addition]
    *** unaffected by: xSenp8(mnr)"CIA" [addition]
    *** source: 15242646-Fig-2b
  *** xTp53[Ab] nedd[tNdAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xNedd8> in BMS
    *** inhibited by: xTp53(F19A) [substitution]
    *** source: 15242646-Fig-4a
  *** xTp53[Ab] nedd[tNdAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xNedd8> in BMS
    *** inhibited by: xUsp7 [addition]
    *** unaffected by: xUsp7(mnr)"CIA" [addition]
    *** source: 15242646-Fig-2c
  *** xTp53[Ab] nedd[tNdAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xNedd8> in BMS
    *** source: 15242646-Fig-2a
  *** xTp53[Ab] nedd[tNdAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xNedd8> in BMS
    *** source: 15242646-Fig-3g
  *** xTp53[Ab] nedd[tNdAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xNedd8> in BMS
    *** unaffected by: xSenp3 [addition]
    *** unaffected by: xSenp3(mnr)"CIA" [addition]
    *** source: 15242646-Fig-2b
  *** xTp53[Ab] nedd[tNdAb-ppt] is increased itpo xMdm2
    *** cells: H1299<Tp53~null><xTp53><xNedd8> in BMS
    *** unaffected by: xTp53(K370R/K372R/K373R) [substitution]
    *** inhibited by: xTp53(K381R/K382R/K386R) [substitution]
    *** inhibited by: xTp53(K370R/K372R/K373R/K381R/K382R/K386R) [substitution]
    *** source: 15242646-Fig-4a

