==============
Ubiquitination
==============

  *** xMdm4[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: HCT116<Chek2~null><xMdm4><xUbiq> in BMS
    *** enhanced by: xChek2 [addition]
    *** unaffected by: xChek2(S347A)"KD" [addition]
    *** source: 16163388-Fig-9b
  *** xMdm4[Ab] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: HCT116<xMdm4><xUbiq> in BMS
    *** enhanced by: xChek2 [addition]
    *** unaffected by: xChek2(S347A)"KD" [addition]
    *** source: 16163388-Fig-9b
  *** xMdm4[tAb] ubiq[tUbAb-ppt] is increased itpo xMdm2
    *** cells: MCF7<xMdm4><xUbiq> in BMS "pretreated with MG132"
    *** source: 15788536-Fig-6c

==================
Protein Expression
==================

  *** xMdm4[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: mEFs<Tp53~null><Mdm2~null><xMdm4> in BMS
    *** reversed by: xMdm2(mnr)"ring-domain-mutant" [substitution]
    *** source: 12963717-Fig-4b
  *** xMdm4[Ab] prot-exp[WB] is decreased itpo xMdm2
    *** cells: mEFs<Tp53~null><Mdm2~null><xMdm4> in BMS
    *** source: 12963717-Fig-4a

  *** Mdm4[Ab] prot-exp[WB] is unchanged itao Mdm2 [RNAi]
    *** cells: MCF7 in BMS
    *** source: 12963717-Fig-3a

=================
Protein Stability
=================

  *** xMdm4[Ab] prot-stability[CHX-WB] is detectable
    *** cells: mEFs<Tp53~null><Mdm2~null><xMdm4><xMdm2> in BMS
    *** reversed by: xMdm2(mnr)"ring-domain-mutant" [substitution]
    *** source: 12963717-Fig-4c

========
Location
========

  *** xMdm4[XFP] locatedin(nucleus)[IHC] is increased itpo xMdm2
    *** cells: mEFs<Tp53~null><Mdm2~null><xMdm4> in BMS
    *** inhibited by: xMdm2(mnr)"del-NLS" [substitution]
    *** unaffected by: xMdm2(mnr)"del-NES" [substitution]
    *** source: 12963717-Fig-4d

