===============
Kinase Activity
===============

requires Cdc42--

  *** xErk1[tAb]IP IVKA(Ets1)[32P-ATP] is increased irt Hgf (10 min)
    *** cells: MDCK in BMLS
    *** unaffected by: xCdc42(T17N) [addition]
    *** source: 11948414-Fig-5a

requires Hras

  *** xErk1[tAb]IP IVKA(Ets1)[32P-ATP] is increased irt Hgf (10 min)
    *** cells: MDCK in BMLS
    *** inhibited by: xHras(S17N) [addition]
    *** source: 11948414-Fig-5a

requires Raf1--

  *** xErk1[tAb]IP IVKA(Ets1)[32P-ATP] is increased irt Hgf (10 min)
    *** cells: MDCK in BMLS
    *** inhibited by: xRaf1(128-203)"DN" [addition]
    *** source: 11948414-Fig-5a

Chemicals

  *** xErk1[tAb]IP IVKA(Ets1)[32P-ATP] is increased irt Hgf (10 min)
    *** cells: MDCK in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** source: 11948414-Fig-6a
  *** Erk1[Ab]IP IVKA(Eif4ebp1)[32P-ATP] is increased irt Hgf (10 min)
    *** cells: NIH3T3 in BMLS
    *** bkg inhibited by: PD98059 [pretreatment]
    *** unaffected by: LY294002 [pretreatment]
    *** source: 1134526-Fig-4d
  *** Erks[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Hgf (15 min)
    *** cells: hBMECs in BMS
    *** bkg inhibited by: U0126 [pretreatment]
    *** source: 17055484-Fig-6b

No extras

  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Hgf (times)
    *** cells: MDCK in BMLS
    *** times: 0 5 15++ 30+ 60+ 120+ 180+ 240+ min
    *** source: 9668053-Fig-1b
  *** Erks[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Hgf (10 min)
    *** cells: rENCNs in BMLS
    *** source: 9681449-Fig-10
  *** xErk1[tAb]IP IVKA(Ets1)[32P-ATP] is increased irt Hgf (10 min)
    *** cells: MDCK in BMLS
    *** source: 11948414-Fig-2b

======================
Phosphorylation on TEY
======================

does not req Mras--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: mAstrocytes in BMLS
    *** times: 0 5+ 15+ 40+ 90+ min
    *** does not req: Mras [KO]
    *** source: 16980617-Fig-7a

Effect of Fak1--

  *** Erks[Ab]IP IVKA(MBP)[32P-ATP] is increased irt Hgf (15 min)
    *** cells: HEK293 in BMLS
    *** enhanced by: xFak1 [addition]
    *** unaffected by: xFak1 + xHras(S17N) [addition]
    *** source: 9748249-Fig-6a

Gab1--  ???

  *** xErk2[tAb]IP phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 10++ 60+ 240 min
    *** unaffected by: xGab1 [addition]
    *** partially inhibited by: xGab1(Y682F)"dShp2" [substitution]
    *** source: 10871282-Fig-6c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: MDCK<xGab1> in BMLS
    *** times: 0 15+ 60+ 180+ min
    *** bkg inhibited by: xGab1(mnr)"delShp2" [substitution]
    *** source: 11046147-Fig-7a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: MDCK in BMLS
    *** times: 0 5 30+ 60+ 180+ min
    *** unaffected by: xGab1 [addition]
    *** source: 12058075-Fig-2d

Gab2--  ???

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: MDCK in BMLS
    *** times: 0 5 30+ 60+ 180+ min
    *** unaffected by: xGab2 [addition]
    *** source: 12058075-Fig-2d

Muc20-- ???

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (5 min)
    *** cells: HEK293 in BMLS
    *** comment: both Erks-Yphos and prot-exp are partially inhibited itpo xMuc20
    *** source: 15314156-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (5 min)
    *** cells: HEK293 in BMLS
    *** partially inhibited by: xMuc20 [addition]
    *** source: 15314156-Fig-2d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (5 min)
    *** cells: mTECs in BMLS
    *** partially inhibited by: xMuc20 [addition]
    *** source: 15314156-Fig-4a


Chemicals--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (5 min)
    *** cells: 3731E in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** source: 19274086-Fig-1c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (10 min)
    *** cells: 3731E in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** unaffected by: LY294002 [pretreatment] no-bkg-control
    *** source: 19274086-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (10 min)
    *** cells: 3731E in BMLS
    *** partially inhibited by: U0126 [pretreatment] no-bkg-control
    *** unaffected by: BID1870 [pretreatment] no-bkg-control
    *** source: 19274086-Fig-1d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (10 min)
    *** cells: 3731E in BMLS "phospho-protein-extract"
    *** partially inhibited by: PHA665752 [pretreatment] no-bkg-control
    *** source: 19274086-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (10 min)
    *** cells: 3731T in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** unaffected by: LY294002 [pretreatment] no-bkg-control
    *** source: 19274086-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (10 min)
    *** cells: MEWO in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** unaffected by: LY294002 [pretreatment] no-bkg-control
    *** source: 19274086-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: U0126 [pretreatment]
    *** source: 14636584-Fig-2d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (10 min)
    *** cells: SKMEL28 in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** unaffected by: LY294002 [pretreatment] no-bkg-control
    *** source: 19274086-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: MIMCD3 in BMLS
    *** times: 0 10+ 30+ min
    *** inhibited by: U0126 [pretreatment]
    *** source: 11784715-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: MDCK in BMLS
    *** times: 0 10++ 240++ 90+ min
    *** inhibited by: PD98059 [pretreatment]
    *** source: 9693375-Fig-4
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: PD98059 [pretreatment] no-bkg-control
    *** source: 1118451-Fig-1a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: U0126 [pretreatment] no-bkg-control
    *** source: 1118451-Fig-1b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: MIMCD3 in BMLS
    *** times: 0 2+ 5+ 10+ min
    *** inhibited by: U0126 [pretreatment]
    *** source: 11445578-Fig-4c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: MIMCD3 in BMS
    *** times: 0 10+ 20+ min
    *** inhibited by: U0126 [pretreatment]
    *** source: 11445578-Fig-1
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (15 min)
    *** cells: hBMECs in BMS
    *** bkg inhibited by: U0126 [pretreatment]
    *** source: 17055484-Fig-6b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (15 min)
    *** cells: hBMECs in BMS
    *** unaffected by: PP2 [pretreatment]
    *** source: 17055484-Fig-6c

No extras

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: B16F1 in BMLS
    *** times: 0+ 1+++ 2++ 4++ 8++ 12+ 24 48 hr
    *** source: 19274086-Fig-1c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: RGM1 in BMLS
    *** times: 0 5+++ 15+++ 30++ 60+ 120 min
    *** source: 15326485-Fig-2f
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (tnr)
    *** cells: 3731T in BMLS
    *** source: 19274086-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (tnr)
    *** cells: B16F1 in BMLS
    *** source: 19274086-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (tnr)
    *** cells: MEWO in BMLS
    *** source: 19274086-Fig-2a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: MDCK in BMLS
    *** times: 0 15+ 60+ 180+ min
    *** source: 11046147-Fig-7
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (15 min)
    *** cells: HELA in BMLS
    *** source: 12147692-Fig-2d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 5+ 10+++ 15+++ 30+++ 60++ 120++ 180+ 240+ min
    *** source: 15314156-Fig-2c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: HEK293<xMuc20> in BMLS
    *** times: 0 5 10+ 15+ 30+ 60+ 120+ 180+ 240+ min
    *** source: 15314156-Fig-2c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: hBMECs in BMS
    *** times: 0+ 1++ 5++ 15++ 30++ 60++ min
    *** source: 17055484-Fig-6a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt Hgf (times)
    *** cells: CHANG in BMLS
    *** times: 0 10++ 30++ 60++ 120++ 240+ 360+ 480+ min
    *** source: 19071214-Fig-1b

====================================================
Recruitment to Pxn complex requires phos on Pxn Y118
====================================================

  *** Erks[Ab] copptby[WB] Pxn[Ab] is increased irt Hgf (10 min)
    *** cells: MIMCD3 in BMLS
    *** comment: coppt Erks are phosed on TEY [phosAb]
    *** source: 14636584-Fig-2a
  *** Erks[Ab] copptby[WB] Pxn[Ab] is increased irt Hgf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: PP1 [pretreatment] no-bkg-control
    *** source: 14636584-Fig-3b
  *** Erks[Ab] copptby[WB] xPxn[tAb] is increased irt Hgf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: xPxn(Y118A/F120V/P121A) [substitution]
    *** inhibited by: xPxn(Y118A) [substitution]
    *** source: 14636584-Fig-4d
  *** Erks[Ab] snaggedby[WB] rPxn(1-323)[GST] is increased irt Hgf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: PP1 [pretreatment]
    *** source: 14636584-Fig-4b
  *** Erks[Ab] snaggedby[WB] rPxn(1-323)[GST] is increased irt Hgf (10 min)
    *** cells: MIMCD3 in BMLS
    *** inhibited by: rPxn((1-323)/Y118A) [substitution]
    *** source: 14636584-Fig-4b
  *** Erks{phos}[phosAb] copptby[WB] Pxn[Ab] is increased irt Hgf (times)
    *** cells: MIMCD3 in BMLS
    *** times: 0 10++ 20++ 30+ 60 180 min
    *** source: 14636584-Fig-2b
  *** Pxn[Ab] copptby[WB] Erks[Ab] is increased irt Hgf (10 min)
    *** cells: MIMCD3 in BMLS
    *** unaffected by: U0126 [pretreatment]
    *** source: 14636584-Fig-2d
