===============================
Relocation to subnuclear-bodies
===============================

Atm-- ???

  *** xTp53bp1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (15 min)
    *** cells: U2OS<xTp53bp1> in BMS
    *** partially reqs: Atm [RNAi]
    *** source: 16009723-Fig-5

H2ax-- at later times only

  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (4 hr)
    *** cells: mEFs in BMS
    *** reqs: H2ax [KO]
    *** source: 12792649-Fig-2b
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (8 min)
    *** cells: mEFs in BMS
    *** does not req: H2ax [KO]
    *** source: 12792649-Fig-1c
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30+ 60++ 240+++ min
    *** comment: does not req H2ax [KO] at 15 min
    *** comment: reqs H2ax [KO] at 60 and 240 min
    *** source: 12792649-Fig-2a
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<H2ax~null><xH2ax> in BMS "treated with IR for 30 min"
    *** reqs: xH2ax [omission]
    *** inhibited by: xH2ax(Y143A) [substitution]
    *** source: 16377563-Fig-5b

not Brca--

  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: hFibros in BMS "treated with IR for 1 hr"
    *** does not req: Brca1 [RNAi]
    *** source: 19001859-Fig-6b
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: U2OS in BMS "treated with MicroLaser for 30 min"
    *** comment: colocates with Brca1[Ab]
    *** does not req: Brca1 [RNAi]
    *** source: 18001824-Fig-S5

Mdc1--

  -------------- reqs phos sites in TQXF motif (T699/T719/T752/T765) ---
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS "treated with IR for tnr"
    *** reqs: xMdc1 [omission]
    *** inhibited by: xMdc1(T699A/T719A/T752A/T765A) [substitution]
    *** source: 18006705-Fig-1f
  ----------------- required ---------------------------
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (4 hr)
    *** cells: U2OS in BMS
    *** reqs: Mdc1 [RNAi]
    *** source: 18583988-Fig-4d
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (4 hr)
    *** cells: U2OS<Mdc1~kd><xMdc1> in BMS
    *** reqs: xMdc1 [omission]
    *** inhibited by: xMdc1(del(196-506)) [substitution] 
    *** source: 18583988-Fig-4d
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS in BMS "treated with IR for 2 hr"
    *** reqs: Mdc1 [RNAi]
    *** source: 12607005-Fig-2c
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs in BMS "treated with IR for tnr"
    *** reqs: Mdc1 [KO]
    *** source: 18678890-Fig-4a
  *** Tp53bp1[tAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: mEFs<Mdc1~null><xMdc1> in BMS "treated with IR for tnr"
    *** unaffected by: xMdc1(del(200-420))"dSDTD" [substitution]
    *** inhibited by: xMdc1(del(698-768))"dTQXF" [substitution]
    *** source: 18678890-Fig-4b
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Mdc1 [RNAi]
    *** source: 16618811-Fig-6b
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is unchanged irt MicroLaser (1 hr)
    *** cells: U2OS<Mdc1~kd> in BMS
    *** source: 16618811-Fig-S3c
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is unchanged irt MicroLaser (1 hr)
    *** cells: U2OS<Mdc1~kd> in BMS
    *** source: 16618811-Fig-6b
  *** xTp53bp1[XFP] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: U2OS<xTp53bp1> in BMS
    *** times: 0 15+ 60+ 360+ min
    *** comment: increase is not seen at 15 and 360 hr itao Mdc1 [RNAi]
    *** source: 16009723-Fig-S1b
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS in BMS "treated with IR for 24 hr"
    *** reqs: Mdc1 [RNAi]
    *** source: 18006705-Fig-S5c
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS in BMS "treated with IR for tnr"
    *** reqs: Mdc1 [RNAi]
    *** source: 18006705-Fig-1f
  ----------------- partially required ---------------------------
  *** xTp53bp1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (15 min)
    *** cells: U2OS<xTp53bp1> in BMS
    *** partially reqs: Mdc1 [RNAi]
    *** source: 16009723-Fig-4b
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: hFibros in BMS "treated with IR for 1 hr"
    *** partially reqs: Mdc1 [RNAi]
    *** source: 19001859-Fig-6b
  ----------------- not required ---------------------------
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 30 min"
    *** does not req: Mdc1 [RNAi]
    *** source: 12607003-Fig-4c
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (tnr)
    *** cells: MCF7 in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 12551934-Fig-2d
  ---------------------- timing ----------------------------------
  *** xTp53bp1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS<xTp53bp1> in BMS
    *** times: 0 30++ 80++ 180++  270++ 360++ 600 720 min
    *** comment: decrease is hastened itao Mdc1 [RNAi]
    *** source: 16009723-Fig-6

Rnf8--

  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS in BMS "treated with IR for 24 hr"
    *** reqs: Rnf8 [RNAi]
    *** source: 18006705-Fig-S5c
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS<Rnf8~kd><xRnf8> in BMS "treated with IR for 1 hr"
    *** reqs: xRnf8 [omission]
    *** inhibited by: xRnf8(R42A) [substitution]
    *** inhibited by: xRnf8(C408S) [substitution]
    *** source: 18006705-Fig-2f
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** reqs: Rnf8 [RNAi]
    *** source: 18006705-Fig-S9a
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** reqs: Rnf8 [RNAi]
    *** source: 18006705-Fig-S9b
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** bkg reqs: Rnf8 [RNAi]
    *** source: 18006705-Fig-2c
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Rnf8 [RNAi]
    *** source: 18006705-Fig-S9c
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: U2OS in BMS
    *** times: 0+ 5++ 15+++ 30+++ min
    *** bkg reqs: Rnf8 [RNAi]
    *** source: 18006705-Fig-S5c

Ubc13--

  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** reqs: Ubc13 [RNAi]
    *** source: 18006705-Fig-S12a
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Ubc13 [RNAi]
    *** source: 18006705-Fig-S12a

not other E2 ligases--

  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: HELA in BMS
    *** does not req: Cdc34 [RNAi]
    *** does not req: Ube2a [RNAi]
    *** does not req: Ube2b [RNAi]
    *** does not req: Ube2d2 [RNAi]
    *** does not req: Ube2d3 [RNAi]
    *** does not req: Ube2e1  [RNAi]
    *** does not req: Ube2e2 [RNAi]
    *** does not req: Ube2e3  [RNAi]
    *** does not req: Ube2l3 [RNAi]
    *** does not req: Ube2l6  [RNAi]
    *** does not req: Ube2v1 [RNAi]
    *** does not req: Ube3a [RNAi]
    *** source: 18006705-Fig-S12a
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Cdc34 [RNAi]
    *** does not req: Ube2a [RNAi]
    *** does not req: Ube2b [RNAi]
    *** does not req: Ube2d2 [RNAi]
    *** does not req: Ube2d3 [RNAi]
    *** does not req: Ube2e1  [RNAi]
    *** does not req: Ube2e2 [RNAi]
    *** does not req: Ube2e3  [RNAi]
    *** does not req: Ube2l3 [RNAi]
    *** does not req: Ube2l6  [RNAi]
    *** does not req: Ube2v1 [RNAi]
    *** does not req: Ube3a [RNAi]
    *** source: 18006705-Fig-S12a

Tp32bp1 features

  *** xTp53bp1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS<xTp53bp1> in BMS
    *** times: 0 1 2+ 4++ 6+++ 8+++ 10++++ 14++++ 16++++ min
    *** inhibited by: xTp53bp1(Y1487L) [substitution]
    *** source: 16009723-Fig-2b
  *** xTp53bp1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS<xTp53bp1> in BMS
    *** inhibited by: xTp53bp1(Y1487L) [substitution]
    *** source: 16618811-Fig-S3a

chemicals

  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 15+ 30++ 60++ min
    *** inhibited by: KU55933 [cotreatment]
    *** unaffected by: NU7026 [cotreatment]
    *** source: 18006705-Fig-S4a

no extras

  *** xTp53bp1[XFP] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: U2OS<xTp53bp1> in BMS
    *** times: 0 1 5+ 20+ min
    *** source: 16009723-Fig-S1a
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is increased irt IR (times)
    *** cells: U2OS in BMS
    *** times: 0 1 5+ 20+ min
    *** source: 16009723-Fig-S1a
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS in BMS
    *** times: 0 1 2 5+ 10++ 20+++ min
    *** source: 16009723-Fig-3c
  *** xTp53bp1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS<xTp53bp1> in BMS
    *** times: 0 1 2+ 4++ 6+++ 8+++ 10++++ 14++++ 16++++ min
    *** source: 16009723-Fig-2a
  *** xTp53bp1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS<xTp53bp1> in BMS
    *** times: 0 1 2+ 4++ 6+++ 8+++ 10++++ 14++++ 16++++ min
    *** source: 16009723-Fig-3a
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: HELA in BMS "treated with IR for 6 hr"
    *** source: 12607003-Fig-4b
  *** xTp53bp1[XFP] locatedin(subnuclear-bodies)[IHC] is increased irt IR (1 hr)
    *** cells: U2OS<xTp53bp1> in BMS
    *** comment: subnuclear bodies are smaller than those occupied by xMdc1
    *** comment: subnuclear bodies are larger than those occupied by xAtr xRad52
    *** source: 16618811-Fig-9b
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt IR (times)
    *** cells: MCF7 in BMS
    *** times: 0 15++++ 30++++ 60+++ 120++ 180+ 360+ min
    *** comment: time measurements are percent cells with foci
    *** source: 12551934-Fig-2a
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: MRC5 in BMS "treated with IR for 30 min"
    *** comment: colocates with Mdc1
    *** source: 12607003-Fig-2c
  *** Tp53bp1[Ab] locatedin(subnuclear-bodies)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** source: 16618811-Fig-S1a
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: BJ in BMS
    *** source: 16618811-Fig-S2
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** source: 16618811-Fig-2a
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS in BMS
    *** comment: colocates with Brca1
    *** source: 16618811-Fig-6b
  *** xTp53bp1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (1 hr)
    *** cells: U2OS<xTp53bp1> in BMS
    *** source: 16618811-Fig-9a

  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is detectable
    *** cells: U2OS in BMS "treated with MicroLaser for 1 hr"
    *** comment: colocates with H2ax{phos(S140)}[phosAb]
    *** reqs: Rnf8 [RNAi]
    *** source: 18001824-Fig-4a
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is increased irt MicroLaser (times)
    *** cells: U2OS in BMS
    *** times: 2 4+ 8+ 12+ 15+ min
    *** source: 18001824-Fig-S4a
  *** Tp53bp1[Ab] locatedin(DSBs)[IHC] is undetectable
    *** cells: U2OS in BMS "treated with MG132 for 90 min followed by MicroLaser for 30 min"
    *** comment: does not colocalize with H2ax{phos(S140)}[phosAb]
    *** source: 18001824-Fig-3c
  *** Tp53bp1[tAb] locatedin(subnuclear-bodies)[IHC] is detectable
    *** cells: U2OS<Rnf8~kd><xRnf8> in BMS "treated with IR for 1 hr"
    *** comment: colocates with H2ax{phos(S140)}[phosAb]
    *** inhibited by: xRnf8(R42A)"mutFHA" [substitution]
    *** inhibited by: xRnf8(C403S)"mutRING" [substitution]
    *** source: 18001824-Fig-5c
  *** xTp53bp1[XFP] locatedin(DSBs)[IHC] is increased irt MicroLaser (14 min)
    *** cells: U2OS<xTp53bp1> in BMS
    *** comment: increase was monitored continuously during MicroLaser
    *** comment: increase was slower than xNbs1 xMdc1 xRnf8 in same system
    *** comment: increase was similar to  xBrca1 in same system
    *** comment: increase was faster than xAtr in same system
    *** source: 18001824-Fig-3b






















