================================================================
Evidence needed for translocation to nucleus and upstream events
================================================================

====================================================
Proteins required for phosphorylation on T69 and T71
====================================================

Jnk1-- and/or Jnk2--

  *** Atf2[phosAb] phos(T69/T71)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs in BMS
    *** partially reqs: Jnk1 and/or Jnk2 [DKO]
    *** source: 15304344-Fig-3b

not P38s-- unless Jnk1 and Jnk2 are KO

  *** Atf2[phosAb] phos(T69/T71)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs in BMS
    *** unaffected by: SB203580 [addition]
    *** source: 15304344-Fig-5a
  *** Atf2[phosAb] phos(T69/T71)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs<Jnk1~null><Jnk2~null> in BMS
    *** inhibited by: SB203580 [addition]
    *** source: 15304344-Fig-5c

==================================================
Proteins required for phosphorylation on T71 alone
==================================================

Jnk1-- and/or Jnk2--

  *** Atf2[phosAb] phos(T71)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs in BMS
    *** bkg reqs: Jnk1 and/or Jnk2 [DKO]
    *** source: 15304344-Fig-3b

not P38s-- unless Jnk1 and Jnk2 are KO

  *** Atf2[phosAb] phos(T71)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs in BMS
    *** unaffected by: SB203580 [addition]
    *** source: 15304344-Fig-5a
  *** Atf2[phosAb] phos(T71)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs in BMS
    *** unaffected by: SB203580 [addition]
    *** unaffected by: PD184352 [addition]
    *** unaffected by: SB203580 + PD184352 [addition]
    *** source: 15304344-Fig-5b
  *** Atf2[phosAb] phos(T71)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs<Jnk1~null><Jnk2~null> in BMS
    *** partially inhibited by: SB203580 [addition]
    *** source: 15304344-Fig-5c
  *** Atf2[phosAb] phos(T71)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs<Jnk1~null><Jnk2~null> in BMS
    *** partially inhibited by: SB203580 [addition]
    *** unaffected by: PD184352 [addition]
    *** inhibited by: SB203580 + PD184352 [addition]
    *** source: 15304344-Fig-5d

not Mek1--

  *** Atf2[phosAb] phos(T71)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs in BMS
    *** unaffected by: PD184352 [addition]
    *** source: 15304344-Fig-5b
  *** Atf2[phosAb] phos(T71)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs<Jnk1~null><Jnk2~null> in BMS
    *** unaffected by: PD184352 [addition]
    *** source: 15304344-Fig-5d

============================================
Proteins required for phosphorylation on S90
============================================

Jnk1-- and/or Jnk2--

  *** Atf2[phosAb] phos(S90)[phosAb] is increased irt Tnf (15 min)
    *** cells: mEFs in BMS
    *** reqs: Jnk1 and/or Jnk2 [DKO]
    *** source: 15304344-Fig-3b

===========
other stuff
===========

  *** Atf2[phosAb] phos(T71)[phosAb] is increased irt Tnf (times)
    *** cells: 3T3L1 in BMS "differentiated into adipocytes"
    *** times: 0 5+ 10++ 15++ 30++ 60+ 120+ 360+ 720+ min
    *** source: 17500068-Fig-5a
  *** Atf2[phosAb] phos(T71)[phosAb] is increased irt Tnf (times)
    *** cells: 3T3L1 in BMS "differentiated into adipocytes"
    *** times: 0 5+ 15++ 30++ 60+ 120+ 360 720 min
    *** source: 17500068-Fig-9a
  *** Atf2[phosAb] phos(T71)[phosAb] is increased irt anti-TnfR1 (times)
    *** cells: 3T3L1 in BMS "differentiated into adipocytes"
    *** times: 0 5 15+ 30++ 60+ 120+ 360 720 min
    *** source: 17500068-Fig-9a
  *** Atf2[phosAb] phos(sitenr)[phosAb] is increased irt Tnf (15 min)
    *** cells: HELA<xTraf2><xUbc13> in BMS
    *** unaffected by: xUbc13(C87A) [substitution]
    *** source: 14713952-Fig-3d
