====================
physical interaction
====================

inhibited by Rheb--

  *** Hras[Ab] copptby[WB] Braf[Ab] is increased itao Rheb [RNAi]
    *** cells: mEFs<Tsc2~null> in BMLS
    *** source: 16803888-Fig-4b

enhanced by Hras GTP binding

  *** Braf[Ab] copptby[WB] xHras[GST] is detectable
    *** cells: HEK293T<xHras> in BMS
    *** enhanced by: xHras(G12V)"CA" [substitution]
    *** source: 16630891-Fig-1b

requires Braf phos(S446)

  *** xBraf[tAb] copptby[WB] Hras[Ab] is detectable
    *** cells: HEK293<xBraf> in BMLS
    *** inhibited by: xBraf(S446A) [substitution]
    *** source: 16803888-Fig-4d

  *** xBraf[tAb] copptby[WB] xHras(G12R)[tAb] is detectable
    *** cells: NIH3T3<xBraf><xHras(G12R)> in BMS
    *** source: 10064593-Fig-4c
  *** xBraf[tAb] copptby[WB] xHras(Q61L)"CA"[tAb] is detectable
    *** cells: COS<xBraf><xHras(Q61L)> in BMS
    *** source: 15632082-Fig-3a
  *** xBraf[tAb] copptby[WB] xHras(Q61L)"CA"[tAb] is detectable
    *** cells: COS<xBraf><xHras(Q61L)> in BMS
    *** source: 15632082-Fig-3b
  *** Braf[Ab] copptby[WB] xHras(Q61L)"CA"[tAb] is undetectable
    *** cells: COS<xHras(Q61L)> in BMS
    *** source: 15632082-Fig-3c
  *** Braf[Ab] copptby[WB] xHras(Q61L)[GST] is detectable
    *** cells: COS<xHras(Q61L)> in BMS
    *** source: 16157584-Fig-5c
  *** xBraf[tAb] copptby[WB] xHras(Q61L)[GST] is detectable
    *** cells: COS<xBraf><xHras(Q61L)> in BMS
    *** source: 16157584-Fig-5a
  *** xHras(Q61L)[tAb] copptby[WB] xBraf[tAb] is detectable
    *** cells: COS<xBraf><xHras(Q61L)> in BMS
    *** source: 16157584-Fig-5b

==========
relocation
==========

  *** xBraf[tAb] locatedin(cellmembrane)[IHC] is increased itpo xHras(G12V)"CA"
    *** cells: MDCK in BMS
    *** source: 9020159-Fig-1

===============
Kinase Activity
===============

Akt1--  ???

  *** xBraf[tAb]IP IVKA(Mek?,Erk?,Elk1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMLS
    *** inhibited by: xAkt1(myr)"CA" [addition] no-bkg-control
    *** source: 11410590-Fig-1
  *** xBraf[tAb]IP IVKA(Erk1,Elk1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293<xBraf> in BMLS
    *** inhibited by: xAkt1 [addition]
    *** enhanced by: xAkt1(mnr)"KD" [addition]
    *** source: 10869359-Fig-1c

Sgk1--  ???

  *** xBraf[tAb]IP IVKA(Mek?,Erk?,Elk1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMLS
    *** bkg inhibited by: xSgk1(S422D)"CA" [addition]
    *** unaffected by: xSgk1(K127M)"KD" [addition]
    *** source: 11410590-Fig-1

Src--  ???

  *** xBraf[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(G12R)"CA"
    *** cells: COS7 in BMS
    *** unaffected by: xSrc(Y527F)"CA" [addition]
    *** source: 9020159-Fig-2b
  *** xBraf[tAb]IP IVKA(Mek2,Erk2,MBP)[32P-ATP] is increased itpo xHras(G12R)"CA"
    *** cells: COS7 in BMS
    *** unaffected by: xSrc(Y527F)"CA" [addition]
    *** source: 9020159-Fig-2b


Hras features

  *** xBraf[Ab]IP IVKA(Mek1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMLS
    *** inhibited by: xHras(G12V/mnr)"membrane-association-mut" [substitution]
    *** source: 11325826-Fig-2

Braf features

  *** xBraf[tAb]IP IVKA(Mek1,Erk1,Elk1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS1 in BMS
    *** inhibited by: xBraf(T598A) [substitution]
    *** partially inhibited by: xBraf(S601A) [substitution]
    *** unaffected by: xBraf(S613A) [substitution]
    *** inhibited by: xBraf(T598A/S601A) [substitution]
    *** source: 11032810-Fig-1b
  *** xBraf[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(G12R)"CA"
    *** cells: COS in BMS
    *** partially inhibited by: xBraf(S445A/S446A) [substitution]
    *** partially inhibited by: xBraf(S447A/S448A) [substitution]
    *** partially inhibited by: xBraf(S445A/S446A/S447A/S448A) [substitution]
    *** source: 10205168-Fig-6b
  *** xBraf[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMS
    *** partially inhibited by: xBraf(T598A/T601A) [substitution]
    *** source: 11447113-Fig-2a

No extras

  *** xBraf[tAb]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293T in BMS
    *** source: 15143186-Fig-1b
  *** xBraf[tAb]IP IVKA(Mek1,Erk2)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: COS7 in BMS
    *** source: 16364920-Fig-4d
  *** Braf[Ab]IP IVKA(Mek1,Erk1)[32P-ATP] is increased itpo xHras(Q61L)"CA"
    *** cells: NIH3T3 in BMS
    *** source: 8887643-Fig-7
  *** xBraf[GST]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(Q61L)"CA"
    *** cells: COS in BMLS
    *** source: 15632082-Fig-4c
  *** xBraf[tAb]IP IVKA(Mek1,Erk?,Elk1)[32P-ATP] is increased itpo xHras(G12V)"CA"
    *** cells: HEK293 in BMS
    *** source: 11447113-Fig-5a
  *** xBraf[GST]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased itpo xHras(Q61L)
    *** cells: COS<xBraf> in BMS
    *** source: 16157584-Fig-5d

==========
phos(T119)
==========

  *** xBraf[phosAb] phos(T119)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: HELA in BMS
    *** source: 16858395(D)

==========
phos(S446)
==========

  *** xBraf[Ab]IP phos(S446)[phosAb] is increased itpo xHras(Q61L)"CA"
    *** cells: PC6 in BMS
    *** source: 15632082-Fig-4b
  *** xBraf[tAb]IP phos(S446)[phosAb] is increased itpo xHras(Q61L)"CA"
    *** cells: COS in BMS
    *** source: 15632082-Fig-4a
  *** xBraf[GST]IP phos(S446)[phosAb] is increased itpo xHras(Q61L)
    *** cells: COS<xBraf> in BMS
    *** source: 16157584-Fig-5d

==========
phos(S599)
==========

  *** xBraf[phosAb] phos(S599)[phosAb] is increased itpo xHras(G12V)"CA"
    *** cells: COS1 in BMS
    *** inhibited by: xBraf(T598A/S601A) [substitution]
    *** source: 11032810-Fig-2e

