=============
oligo-binding
=============

not Pkr--

  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifng (2 hr)
    *** cells: mEFs in BMLS
    *** does not req: Pkr [KO]
    *** source: 9029059-Fig-2b

IfngR2--

  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifng (15 min)
    *** cells: CHOK1<xHlab7><xIfngR1><xIfngR2> in BMS "nuclear extracts"
    *** reqs: xIfngR2 [omission]
    *** inhibited by: xIfngR2(1-285) [substitution]
    *** source: 7673114-Fig-5

Stat1 features

  *** xStat1[RE] oligo-binding[EMSA] is increased irt Ifng (times)
    *** cells: mEFs<Stat1~null> in BMS
    *** times: 0 30++ 120+ 360 720 min
    *** enhanced by: xStat1(K703R) [substitution]
    *** comment: xStat1(K703R) phos is extended to 720 min
    *** unaffected by: xStat1(E705A) [substitution]
    *** source: 16857984-Fig-3c

chemicals

  *** Stat1[RE] oligo-binding[EMSA] is increased irt Ifng (30 min)
    *** cells: HELA in BMS "nuclear-extract"
    *** unaffected by: N6-Methyl-2-deoxyadenosine [addition]
    *** source: 15153491-Fig-6b

no extras

  *** xStat1(L407A)[Ab] oligo-binding[EMSA] is increased irt Ifng (30 min)
    *** cells: U3A<Stat1~null><xStat1> in BMS
    *** source: 11927559-Fig-2b
  *** Stat1[Ab] oligo-binding[EMSA] is detectable
    *** cells: HEP2 in BMS "nuclear extracts from Ifng treated (15 min) cells"
    *** source: 7673114-Fig-5
  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifng (1 hr)
    *** cells: hTh1 in BMS
    *** source: 10037767-Fig-4
  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifng (1 hr)
    *** cells: hTh2 in BMS
    *** source: 10037767-Fig-4
  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifng (20 min)
    *** cells: HT2 in BMS
    *** oligo: "Irf1-GAS" 5-GCCTGATTTCCCCGAAATGACGG-3
    *** source: 8810328-Fig-2b
  *** xStat1[Ab] oligo-binding[EMSA] is increased irt Ifng (20 min)
    *** cells: U3A<Stat1~null><xStat1> in BMS
    *** source: 11080165-Fig-1c
  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifng (30 min)
    *** cells: HEK293 in BMS
    *** source: 12070153-Fig-1a
  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifng (30 min)
    *** cells: HEK293<xEgfR> in BMS
    *** source: 12070153-Fig-1a
  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifng (30 min)
    *** cells: HELA in BMS
    *** comment: unaffected by addition of rKpna1(404-538) during binding
    *** source: 11927559-Fig-7c
  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifng (45 min)
    *** cells: T47D in BMS
    *** source: 7523403-Fig-1a
  *** Stat1[Ab] oligo-binding[EMSA] is unchanged irt Ifng (1 hr)
    *** cells: hTh1 in BMS
    *** source: 10037767-Fig-4
  *** Stat1[Ab] oligo-binding[EMSA] is unchanged irt Ifng (1 hr)
    *** cells: hTh2 in BMS
    *** source: 10037767-Fig-4
  *** Stat1[Ab] snaggedby[WB] oligo[biotin] is increased irt Ifng (30 min)
    *** cells: HEK293<xSumo1><xUbe2i> in BMS
    *** oligo: GAS sequence-not-reported
    *** comment: snagged Stat1 is not Sumoylated [WBMS]
    *** source: 16857984-Fig-1b
