======================
Rgl1 is a Gef for Rala
======================

  *** xRala[tAb]IP GDP-dissociation[3H-GDP] is increased by rRgl1
    *** cells: none
    *** IPfrom: SF9 in BMS "cytosolic-fraction"
    *** comment: response from mem-fraction IP is larger than cyto-fraction IP
    *** source: 9099691-Fig-6a
  *** xRala[tAb]IP GDP-dissociation[3H-GDP] is increased by rRgl1
    *** cells: none
    *** IPfrom: SF9 in BMS "membrane-fraction"
    *** comment: response from mem-fraction IP is larger than cyto-fraction IP
    *** source: 9099691-Fig-6a
  *** xRala[tAb]IP GTP-association[35S-GTPgS] is increased by rRgl1
    *** cells: none
    *** IPfrom: SF9 in BMS "cytosolic-fraction"
    *** comment: response from mem-fraction IP is larger than cyto-fraction IP
    *** source: 9099691-Fig-5
  *** xRala[tAb]IP GTP-association[35S-GTPgS] is increased by rRgl1
    *** cells: none
    *** IPfrom: SF9 in BMS "membrane-fraction"
    *** comment: response from mem-fraction IP is larger than cyto-fraction IP
    *** source: 9099691-Fig-5
  *** xRala[tAb]IP GTP-association[35S-GTPgS] is increased by rRgl1
    *** cells: none
    *** IPfrom: SF9 in BMS "cytosolic-fraction"
    *** comment: response from mem-fraction IP is larger than cyto-fraction IP
    *** source: 9099691-Fig-6b
  *** xRala[tAb]IP GTP-association[35S-GTPgS] is increased by rRgl1
    *** cells: none
    *** IPfrom: SF9 in BMS "membrane-fraction"
    *** comment: response from mem-fraction IP is larger than cyto-fraction IP
    *** source: 9099691-Fig-6b
  *** xRala[tAb]IP GTP-association[35S-GTPgS] is increased by rRgl1(1-505)
    *** cells: none
    *** IPfrom: SF9 in BMS "cytosolic-fraction"
    *** comment: response from mem-fraction IP is larger than cyto-fraction IP
    *** inhibited by: rRgl1(88-505) [substitution]
    *** source: 9099691-Fig-7
  *** xRala[tAb]IP GTP-association[35S-GTPgS] is increased by rRgl1(1-505)
    *** cells: none
    *** IPfrom: SF9 in BMS "membrane-fraction"
    *** comment: response from mem-fraction IP is larger than cyto-fraction IP
    *** inhibited by: rRgl1(88-505) [substitution]
    *** source: 9099691-Fig-7

  *** xRala[tAb]IP GTP-association[TLC] is increased itpo xRgl1
    *** cells: COS in BMS
    *** enhanced by: xHras(G12V) [addition]
    *** unaffected by: xHras(Y40C) [addition]
    *** source: 9099691-Fig-8
  *** xRala[tAb]IP GTP-association[TLC] is increased itpo xRgl1
    *** cells: COS in BMS
    *** partially inhibited by: xRgl1(1-505) [substitution]
    *** source: 9099691-Fig-8

  *** xRala[tAb] GTP-association[BDPD] is increased itpo xRgl1
    *** cells: HEK293T in BMS
    *** enhanced by: xNras(G12V)"CA" [addition]
    *** enhanced by: xRras(G38V)"CA" [addition]
    *** enhanced by: xRras2(G23V)"CA" [addition]
    *** enhanced by: xMras(Q71L)"CA" [addition]
    *** enhanced by: xKras(G12V)"CA" [addition]
    *** enhanced by: xRit1(Q79L)"CA" [addition]
    *** unaffected by: xRin1(A78L) [addition]
    *** unaffected by: xRap1a(G12V)"CA" [addition]
    *** unaffected by: xRap2a(G12V)"CA" [addition]
    *** comment: ranked in order of size of effect
    *** source: 15143186-Fig-2a

===========================
Rgl1 is not a Rala effector
===========================

  *** rRala{GTPgS}[35S] boundby[WB] rRgl1(602-768)[Resin] is undetectable
    *** cells: none
    *** inhibited by: rHras{GDP} [substitution]
    *** source: 7498475-Fig-1











