======================================
semi-direct phosphorylation by TgfbR2
======================================

  *** rSmad2[32P] phos[32P-ATP] is increased by TgfbR1[Ab]IP
    *** cells: none
    *** IPfrom: AKR2B in BMLS "treated with Tgfb1 for 60 min"
    *** source: 14612425-Fig-2c
  *** rSmad2[32P] phos[32P-ATP] is increased by TgfbR2[Ab]IP
    *** cells: none
    *** IPfrom: MV1LU in BMS "treated with Tgfb1 (30 min)"
    *** comment: does not increase by IPfrom MV1LU without TgfbR1
    *** source: 8980228-Fig-2a
  *** rSmad2[32P] phos[32P-ATP] is increased by xTgfbR2[tAb]IP
    *** cells: none
    *** IPfrom: COS1<xTgfbR1><xTgfbR2> in BMS "treated with Tgfb1 (15 min)"
    *** inhibited by: xTgfbR2(mnr)"KD" [substitution]
    *** unaffected by: xTgfbR1(mnr)"KD" [substitution]
    *** source: 8980228-Fig-5a
  *** rSmad2[32P] phos[32P-ATP] is increased by xTgfbR2[tAb]IP
    *** cells: none
    *** IPfrom: COS1<xTgfbR1><xTgfbR2> in BMS "treated with Tgfb1 (30 min)"
    *** source: 8980228-Fig-3b

========================
No Phosphorylation on T8
========================

  *** xSmad2[tAb]IP phos(T8)[phosAb] is unchanged irt Tgfb1 (1 hr)
    *** cells: HEK293T<xSmad3> in BMS
    *** source: 17035229-Fig-2a

==========================
No Phosphorylation on T220
==========================

  *** xSmad2[tAb]IP phos(T220)[phosAb] is unchanged irt Tgfb1 (1 hr)
    *** cells: HEK293T<xSmad3> in BMS
    *** source: 17035229-Fig-2a

=======================
Phosphorylation on S240
=======================

  *** xSmad2[phosAb] phos(S240)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: HEPG2 in BMS
    *** inhibited by: Ro318220 [addition]
    *** source: 11027280(D)
  *** xSmad2[phosAb] phos(S240)[phosAb] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: HEK293 in BMS
    *** source: 11027280(D)

============================
Phosphorylation on S465/S467
============================

not Akt1--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: xAkt1 [addition]
    *** source: 16288034-Fig-3

not Csbk1g2--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS
    *** times: 0 30+ 60+ min
    *** unaffected by: xCsnk1g2 [addition]
    *** source: 18794808-Fig-4b

not Ctdsp1-- Ctdsp2-- CtdspL--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** unaffected by: Ctdsp1 and/or Ctdsp2 [RNAi]
    *** source: 17085434-Fig-5b
  *** xSmad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: HEK293 in BMS
    *** enhanced by: xCtdsp1 [addition]
    *** unaffected by: xCtdsp1(D97E)"CIA" [addition]
    *** enhanced by: xCtdsp2 [addition]
    *** unaffected by: xCtdsp2(D107E)"CIA" [addition]
    *** enhanced by: xCtdspL [addition]
    *** unaffected by: xCtdspL(D112E)"CIA" [addition]
    *** source: 17085434-Fig-2c

Dnm1--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: AKR2B in BMLS
    *** inhibited by: xDnm1(K44A)"DN" [addition]
    *** source: 16288034-Fig-4a

not Erbin--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: xErbin [addition]
    *** source: 19289088-Fig-4a

not Fak1--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (2 hr)
    *** cells: mEFs in BMLS
    *** does not req: Fak1 [KO]
    *** source: 21454615-Fig-1a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (2 hr)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** does not req: xFak1 [omission]
    *** source: 21454615-Fig-1a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs in BMLS
    *** does not req: Fak1 [KO]
    *** source: 21454615-Fig-1b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** does not req: xFak1 [omission]
    *** source: 21454615-Fig-1b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** does not req: xFak1 [omission]
    *** source: 21454615-Fig-2b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** does not req: xFak1 [omission]
    *** unaffected by: xFak1(Y397F) [substitution]
    *** unaffected by: xFak1(P712A/P713A) [substitution]
    *** unaffected by: xFak1(P872A/P873A) [substitution]
    *** unaffected by: xFak1(Y397F/P712A/P713A) [substitution]
    *** unaffected by: xFak1(Y397F/P872A/P873A) [substitution]
    *** source: 21454615-Fig-8b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** does not req: xFak1 [omission]
    *** unaffected by: xFak1(Y397F) [substitution]
    *** unaffected by: xFak1(Y397F/K454R) [substitution]
    *** source: 21454615-Fig-6b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** does not req: xFak1 [omission]
    *** unaffected by: xFak1(Y397F) [substitution]
    *** unaffected by: xFak1(Y576F/Y577F) [substitution]
    *** unaffected by: xFak1(Y397F/K454R) [substitution]
    *** source: 21454615-Fig-6c
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** does not req: xFak1 [omission]
    *** unaffected by: xFak1(Y397F/P712A/P713A/P872A/P873A) [substitution]
    *** source: 21454615-Fig-8d
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Fak1~null><xFak1> in BMLS
    *** unaffected by: xFak1(P712A/P713A/P872A/P873A) [substitution]
    *** unaffected by: xFak1(Y397F/P712A/P713A/P872A/P873A) [substitution]
    *** source: 21454615-Fig-8c
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 30++ 60++ 120++ 240++ min
    *** does not req: Fak1 [KO]
    *** source: 21454615-Fig-2a

not Hras--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb2
    *** cells: AKR2B in BMLS
    *** unaffected by: Hras(S17N)"DN" [addition]
    *** source: 17440079-Fig-3a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: EPH4 in BMS
    *** unaffected by: xHras(mnr)"vRas" [addition]
    *** source: 10197981-Fig-2c

not Merlin--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B<xErbin> in BMLS
    *** does not req: Merlin [RNAi]
    *** source: 19289088-Fig-4a

not Mlst8--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: mEFs in BMLS
    *** does not req: Mlst8 [KO]
    *** source: 19117990-Fig-4a

not Pak2--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: xPak2 [addition]
    *** unaffected by: xPak2(K278R)"KD" [addition]
    *** source: 14612425-Fig-4
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: MV1LU in BMLS
    *** unaffected by: xPak2 [addition]
    *** unaffected by: xPak2(mnr)"DN" [addition]
    *** source: 19289088-Fig-S1b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** unaffected by: xPak2 [addition]
    *** unaffected by: xPak2(mnr)"DN" [addition]
    *** source: 19289088-Fig-S1a

not Pi3kr--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (120 min)
    *** cells: COS7 in BMLS
    *** does not req: Pik3r? [AntiSense]
    *** source: 16288034-Fig-5d

Rac1--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: PANC1 in BMS
    *** times: 0 40+ 100+ min
    *** partially inhibited by: xRac1(S17N)"DN" [addition]
    *** source: 21624123-Fig-6

not Smad3--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: mEFs<Smad3~null> in BMLS
    *** source: 14612425-Fig-3

not Src-- Yes1-- Fyn--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: mEFs<Src~null><Yes1~null><Fyn~null> in BMLS
    *** source: 21454615-Fig-5a

not Tsc2--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: mEFs<Tsc2~null><xTsc2><xS6k1> in BMLS
    *** unaffected by: xTsc2(S939A/T1462A) [substitution]
    *** source: 19117990-Fig-2c

inhbited by Ppm1a--

  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: NIH3T3 in BMS
    *** inhibited by: xPpm1a [addition]
    *** source: 16751101-Fig-S8b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (tnr)
    *** cells: HACAT in BMS
    *** does not req: Ppm1a [RNAi]
    *** source: 16751101-Fig-4b
  *** xSmad2[tAb]IP phos(S465/S467)[phosAb] is increased itpo xTgfbR1(T202D)
    *** cells: HEK293T in BMS
    *** inhibited by: xPpm1a [addition]
    *** source: 16751101-Fig-2a
  *** xSmad2[tAb]IP phos(S465/S467)[phosAb] is increased itpo xTgfbR1(T202D)
    *** cells: HEK293T in BMS
    *** unaffected by: xPpp1ca [addition]
    *** unaffected by: xPpp2ca [addition]
    *** unaffected by: xDusp1 [addition]
    *** source: 16751101-Fig-2a

Smad2 features

  *** xSmad2[tAb]IP phos[32Pi] is increased itpo xTgfbR1(mnr)"CA"
    *** cells: COS1 in BMS
    *** inhibited by: xSmad2(S464A) [substitution]
    *** inhibited by: xSmad2(S465A) [substitution]
    *** inhibited by: xSmad2(S467A) [substitution]
    *** inhibited by: xSmad2(S464A/S465A) [substitution]
    *** inhibited by: xSmad2(S464A/S467A) [substitution]
    *** inhibited by: xSmad2(S465A/S467A) [substitution]
    *** inhibited by: xSmad2(S464A/S465A/S467A) [substitution]
    *** source: 9346908-Fig-2a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is unchanged irt Tgfb1 (120 min)
    *** cells: mEFs<Smad3~null><xAkt1><xSmad2(S467A)> in BMLS
    *** source: 16288034-Fig-3
  *** xSmad2[Ab]IP phos[32Pi] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS1<xSmad2> in BMS
    *** inhibited by: xSmad2(S464A/S465A/S467A) [substitution]
    *** source: 8980228-Fig-4d

chemicals

  *** Smad2[Ab]IP phos(S465/S467)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: RGM1 in BMLS "treated with PD169316"
    *** source: 15326485-Fig-4b
  *** Smad2[Ab]IP phos(S465/S467)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: RGM1 in BMLS "treated with SP600125"
    *** source: 15326485-Fig-4a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (6 hr)
    *** cells: AKR2B in BMLS
    *** unaffected by: CHX [pretreatment]
    *** source: 19117990-Fig-2d
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb2 (1 hr)
    *** cells: ATDC5 in BMLS
    *** unaffected by: U0126 [addition]
    *** unaffected by: SB203580 [addition]
    *** source: 11278290-Fig-5b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: AKR2B in BMLS
    *** unaffected by: LY294002 [pretreatment]
    *** unaffected by: U0126 [pretreatment]
    *** unaffected by: Rapamycin [pretreatment]
    *** source: 19117990-Fig-2a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS
    *** times: 0 90+++ 180++ 360+ min
    *** comment: total protein levels were not reported
    *** comment: decrease in phos at late times is partially inhibited by (Vanadate + OkadaicAcid) [addition]
    *** comment: decrease in phos at late times is inhibited by MG132 [addition]
    *** comment: decrease in phos at late times is inhibited by Lactacystin [addition]
    *** source: 10587642-Fig-1b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: COS1 in BMS
    *** times: 0 1++ 5+ hr
    *** comment: decrease in phos at late times is inhibited by MG132 [addition]
    *** source: 10587642-Fig-1c
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HELA in BMS
    *** times: 0 1++ 5+ hr
    *** comment: decrease in phos at late times is inhibited by MG132 [addition]
    *** source: 10587642-Fig-1c
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HEPG2 in BMS
    *** times: 0 1++ 5+ hr
    *** comment: decrease in phos at late times is inhibited by MG132 [addition]
    *** source: 10587642-Fig-1c
  *** xSmad2[tAb]IP phos(S465/S467)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: COS1 in BMS
    *** enhanced by: Lactacystin [addition]
    *** source: 10587642-Fig-4a
  *** xSmad2(248-467)[tAb]IP phos(S465/S467)[phosAb] is increased irt Tgfb1 (6 hr)
    *** cells: COS1 in BMS
    *** enhanced by: Lactacystin [addition]
    *** source: 10587642-Fig-4b
  *** xSmad2[tAb]IP phos(S465/S467)[phosAb] is increased itpo xTgfbR1(T202D)
    *** cells: HEK293T in BMS
    *** unaffected by: MG132 [addition]
    *** source: 16751101-Fig-2e
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: AKR2B in BMLS
    *** unaffected by: CytochalasinD [pretreatment]
    *** source: 21454615-Fig-5b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 15+ 30+  min
    *** unaffected by: LY294002 [pretreatment]
    *** source: 16288034-Fig-2a

no extras

  *** Smad2[Ab]IP phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: RGM1 in BMLS
    *** times: 0 5+ 15++ 30+++ 60++ 120+ min
    *** source: 15326485-Fig-1b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS
    *** times: 0 15+ 30++ 45++ 60++ 90++ 120++ 180+ min
    *** comment: total protein levels were not reported
    *** source: 10587642-Fig-1a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb2 (times)
    *** cells: ATDC5 in BMLS
    *** times: 0 5++ 15++ 60++ 120+ 240+ min
    *** source: 11278290-Fig-2a
  *** Smad2[Ab]IP phos(S465/S467)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: RGM1 in BMLS
    *** source: 15326485-Fig-1d
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 30++ 60++ 120++ 240+ 360+ 720+ 1440+ min
    *** source: 19117990-Fig-1a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: MV1LU in BMLS
    *** times: 0 30+++ 60+++ 120++ 240++ 360++ 720++ 1440+ min
    *** source: 19117990-Fig-1a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: SMAT1 in BMS
    *** source: 10854429(D)
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** source: 17085434-Fig-1b
  *** xSmad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: HACAT in BMS
    *** source: 11016919-Fig-4
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: HACAT in BMS
    *** source: 11016919-Fig-4
  *** xSmad2[tAb]IP phos(S465/S467)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: HEK293T<xSmad3> in BMS
    *** source: 17035229-Fig-2a
  *** xSmad2[tAb]IP phos(S465/S467)[phosAb] is increased irt Tgfb1 (tnr)
    *** cells: HEK293T in BMS
    *** source: 16751101-Fig-2i
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS
    *** times: 0 1+++ 2++ 4++ 8+ 16+ 24+ hr
    *** source: 16751101-Fig-1a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HEPG2 in BMLS
    *** times: 0 1+ 3++ 6++ 12++ 24 hr
    *** source: 16014625(D)
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS "cytosolic-extracts"
    *** source: 17085434-Fig-1d
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS "nuclear-extracts"
    *** source: 17085434-Fig-1d
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (4 hr)
    *** cells: AKR2B in BMLS
    *** source: 21454615-Fig-5a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: AKR2B in BMLS
    *** source: 21454615-Fig-7b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: HELA in BMLS
    *** source: 21454615-Fig-7b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: MDCK in BMLS
    *** source: 21454615-Fig-7b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: MV1LU in BMLS
    *** source: 21454615-Fig-7b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: NIH3T3 in BMLS
    *** source: 21454615-Fig-7b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (45 min)
    *** cells: SWISS3T3 in BMLS
    *** source: 21454615-Fig-7b
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (times)
    *** cells: mEFs in BMLS
    *** times: 0 10+ 30+ 60+ 120+ 240+ min
    *** source: 21454615-Fig-7a
  *** Smad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (5 min)
    *** cells: AKR2B in BMLS
    *** source: 19289088-Fig-1c
  *** xSmad2[phosAb] phos(S465/S467)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: PANC1<xSmad2> in BMS
    *** source: 21624123-Fig-7b

=====================================
Phosphorylation on an unreported site
=====================================

  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: BXPC3<Smad4~null> in BMS "treated with CHX"
    *** times: 0 60++ 90+ 120+ 180+ 240++ min
    *** comment: phos site is proposed to be S465/S467 by curator
    *** source: 12191474-Fig-5b
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS
    *** times: 0 60++ 90++ 120++ 180+ 240 min
    *** comment: phos site is proposed to be S465/S467 by curator
    *** source: 12191474-Fig-3c
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS "nuclear-extracts"
    *** times: 0 1 5 10+ 15+ 20++ 30++ 45++ 60++ 120++ 180+ 240+ 300+ 360 420 480 min
    *** comment: decrease in signal after 120 min is faster itpo of CHX
    *** comment: phos site is proposed to be S465/S467 by curator
    *** source: 12191474-Fig-1a
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HELA in BMS
    *** times: 0 15+ 30+ min
    *** source: 12356868-Fig-1
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (2 hr)
    *** cells: HEP3B in BMS
    *** partially reqs: Zfyve16 [RNAi]
    *** source: 17272273-Fig-5a
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HEK293T in BMS
    *** times: 0 2 5+ 15++ min
    *** inhibited by: SB431542 [pretreatment]
    *** source: 18758450-Fig-S3a
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HEK293T in BMS
    *** times: 0 15+ 30+ 60+ min
    *** source: 18758450-Fig-2a
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: mEFs in BMS
    *** times: 0 2+ 5++ 15+++ min
    *** comment: no total protein control
    *** does not req: Traf6 [KO]
    *** source: 18758450-Fig-5a
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30+ 60+ min
    *** does not req: Traf6 [KO]
    *** source: 18758450-Fig-S5c
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: PC3U in BMS
    *** times: 0 15+ 30+ 60+ 120++ min
    *** unaffected by: xTgfbR1(mnr/E161A)"CA-Traf6-binding-mut" [addition]
    *** source: 18758450-Fig-S4f
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: PC3U<xTgfbR1><xPkcz> in BMS
    *** inhibited by: SB505124 [cotreatment]
    *** source: 21629263-Fig-5f
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: PC3U<xTgfbR1> in BMS
    *** times: 0 30++ 360+ min
    *** unaffected by: xTgfbR1(E161A) [substitution]
    *** source: 21629263-Fig-2a
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: PC3U<xTgfbR1> in BMS
    *** times: 0 30++ 360+ min
    *** unaffected by: xTgfbR1(G120I) [substitution]
    *** source: 21629263-Fig-4a

==========
total phos
==========

  *** xSmad2[tAb]IP phos[32Pi] is increased irt Tgfb1 (45 min)
    *** cells: NIH3T3 in BMS
    *** bkg inhibited by: xCav1 [addition]
    *** source: 11102446(D)
  *** Smad2[Ab]IP phos[32Pi] is increased irt Tgfb1 (tnr)
    *** cells: HSC4 in BMS
    *** source: 9311995-Fig-3b
  *** Smad3[Ab]IP phos[32Pi] is increased irt Tgfb1 (tnr)
    *** cells: HSC4 in BMS
    *** source: 9311995-Fig-3b
  *** Smad4[Ab]IP phos[32Pi] is unchanged irt Tgfb1 (tnr)
    *** cells: HSC4 in BMS
    *** source: 9311995-Fig-3b
  *** Smad2[Ab]IP phos[32Pi] is increased irt Tgfb1 (tnr)
    *** cells: MV1LU in BMS
    *** source: 9311995-Fig-3a
  *** xSmad2[Ab]IP phos[32Pi] is increased irt Tgfb1 (tnr)
    *** cells: COS1<xTgfbR1><xTgfbR2> in BMS
    *** source: 9335507-Fig-4a
  ---------------------------------------------------------------------
  *** xSmad2[tAb]IP phos[32Pi] is increased itpo xTgfbR1(mnr)"CA"
    *** cells: COS1 in BMS
    *** inhibited by: xTgfbR1(WT) [substitution]
    *** source: 9748228(D)
  *** xSmad2[tAb]IP phos[32Pi] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS1 in BMS
    *** inhibited by: xTgfbR1(WT) [substitution]
    *** source: 9865696-Fig-3a
  *** xSmad2[tAb]IP phos[32Pi] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS1 in BMS
    *** unaffected by: xSara [addition]
    *** source: 9865696-Fig-3a
  *** xSmad2[tAb]IP phos[32Pi] is increased itpo xTgfbR1(mnr)"CA"
    *** cells: COS1 in BMS
    *** source: 9346908-Fig-4
  ---------------------------------------------------------------------

=====================================
Smad2 phos is inhibited itpo of Smad7
=====================================

  *** xSmad2[Ab]IP phos[32Pi] is increased itpo (xTgfbR1 + xTgfbR2)
    *** cells: COS1 in BMS
    *** partially inhibited by: xSmad7 [addition]
    *** source: 9335507-Fig-4b
  *** xSmad2[tAb]IP phos[32Pi] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS1 in BMS
    *** inhibited by: xSmad7 [addition]
    *** source: 9215638-Fig-4
  *** xSmad2[tAb]IP phos[32Pi] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS1 in BMS
    *** inhibited by: xSmad7 [addition]
    *** source: 9215638(D)

=============
phos(sitenr)
============

  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: HELA in BMS
    *** unaffected by: xDnm1(K44R)"DN" [addition]
    *** source: 12034739(D)
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: L17<xTgfbR1> in BMS
    *** unaffected by: Wortmannin [addition]
    *** source: 12034739(D)
  *** xSmad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: HEP3B in BMLS
    *** times: 0 5+ 15+++ 30+++ 60+++ 90+++ 180++ 360+ min
    *** unaffected by: xVps39 [addition]
    *** source: 12941698-Fig-7a,7b
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: MV1LU in BMS
    *** times: 0 10+ 30++ 60++ min
    *** unaffected by: Nystatin [addition]
    *** source: 12717440-Fig-3b
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 5+ 10++ 20++ 40++ 80++ min
    *** repressed by: Cav1 [AntiSense]
    *** source: 11102446(D)
  *** xSmad2[tAb]IP phos(sitenr)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: HELA in BMS
    *** unaffected by: xDnm1(K44R)"DN" [addition]
    *** unaffected by: xSmad2(N381S) [substitution]
    *** source: 12034739(D)
  *** Smad2[Ab]IP phos(sitenr)[phosAb] is increased irt Tgfb1 (tnr)
    *** cells: HEP3B in BMLS "treated with TrichostatinA"
    *** source: 17478422-Fig-1c
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (15 min)
    *** cells: MV1LU in BMLS "nuclear-extracts"
    *** comment: no total Smad2 controls were performed
    *** reqs: Dynlrb1 [RNAi]
    *** source: 17420258-Fig-6
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (24 hr)
    *** cells: BXPC3<Smad4~null> in BMS "cytoplasmic-extracts"
    *** source: 17638924-Fig-1
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (24 hr)
    *** cells: BXPC3<Smad4~null> in BMS "nuclear-extracts"
    *** source: 17638924-Fig-1
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (24 hr)
    *** cells: CAPAN1<Smad4~null> in BMS "cytoplasmic-extracts"
    *** source: 17638924-Fig-1
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (24 hr)
    *** cells: CAPAN1<Smad4~null> in BMS "nuclear-extracts"
    *** source: 17638924-Fig-1
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: MV1LU in BMLS "nuclear-extracts"
    *** times: 0 5+ 10++ 15++ min
    *** comment: no total Smad2 controls were performed
    *** reqs: Dynlrb1 [RNAi]
    *** source: 17420258-Fig-6
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: FAO in BMS
    *** times: 0 1++++ 4+++ 8+++ 12++ 16++ 20+ 24+ hr
    *** source: 15069087-Fig-7a
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: HEPG2 in BMS
    *** enhanced by: xPmlc [addition]
    *** source: 15356634-Fig-S2e
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: mEFs in BMS
    *** reqs: Pml [KO]
    *** source: 15356634-Fig-S2c
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb1 (times)
    *** cells: mMesangialCells in BMLS
    *** times: 0 5 15+ min
    *** does not req: Tab1 [RNAi]
    *** source: 19556242-Fig-5c
  *** xSmad2[tAb]IP phos(sitenr)[phosAb] is increased itpo xTgfbR1(mnr)"CA"
    *** cells: HEK293T in BMS
    *** inhibited by: xSmad2(mnr)"Cterm-3S-3A" [substitution]
    *** unaffected by: xEp300 [addition]
    *** source: 17478422-Fig-4a
  *** xSmad2[tAb]IP phos(sitenr)[phosAb] is increased itpo xTgfbR1(mnr)"CA"
    *** cells: HEK293T in BMS
    *** unaffected by: xSmad2(K19R/K20R/K39R) [substitution]
    *** unaffected by: xEp300 [addition]
    *** source: 17478422-Fig-4c
  *** Smad23s[Ab]IP phos(sitenr)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: U4A<Jak1~null><xJak1> in BMS
    *** unaffected by: ALLN [addition]
    *** source: 11389444-Fig-2a
  *** xSmad2[phosAb] phos(sitenr)[phosAb] is increased itpo xTgfbR1(mnr)"CA"
    *** cells: HEK293T in BMS
    *** unaffected by: ALLN [addition]
    *** source: 11389444-Fig-2c
  *** xSmad2[phosAb] phos(sitenr)[phosAb] is increased itpo xTgfbR1(mnr)"CA"
    *** cells: HEK293T in BMS
    *** unaffected by: xSmurf2 [addition]
    *** source: 11389444-Fig-2c
  *** Smad2[Ab]IP phos(sitenr)[phosAb] is increased irt Tgfb1 (90 min)
    *** cells: HACAT in BMS
    *** source: 18955504-Fig-S5b
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb2 (1 hr)
    *** cells: HEK293<xIL1R1><xIL1Rap> in BMS "pretreated with CHX"
    *** does not req: IL1R1 [RNAi]
    *** reqs: TgfbR1 [RNAi]
    *** reqs: TgfbR2 [RNAi]
    *** unaffected by: xIkba(S32A/S36A)"SR" [addition]
    *** source: 17360530-Fig-5a
  *** Smad2[phosAb] phos(sitenr)[phosAb] is increased irt Tgfb2 (times)
    *** cells: HEK293<xIL1R1><xIL1Rap> in BMS "pretreated with CHX"
    *** times: 0+ 5+ 15+ 30+ 60++ 120+++ min
    *** source: 17360530-Fig-2a


------- contradiction ------------------------------------------------
  *** Smad2[phosAb] phos(sitenr)[phosAb] is detectable-but unchanged irt Tgfb1 (times)
    *** cells: mMesangialCells in BMLS
    *** times: 0+ 2+ 5+ 15+ min
    *** source: 19556242-Fig-3b
  ---------------------------------------------------------------------

  *** xSmad2[Ab]IP phos[32Pi] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS1<xSmad2> in BMS
    *** inhibited by: xTgfbR1 [substitution]
    *** source: 8980228-Fig-4d
  *** xSmad2[tAb]IP phos[32Pi] is increased irt Tgfb1 (15 min)
    *** cells: MV1LU in BMS
    *** comment: phos is mostly on serine by phosphoamino acid analysis
    *** source: 8980228-Fig-2b

============================
Phosphorylation on S250/S255 ???
============================

  *** Smad2[Ab]IP phos(S250/S255)[phosAb] is increased irt Tgfb1 (times)
    *** cells: RGM1 in BMLS
    *** times: 0 5+ 15++ 30+++ 60++ 120+ min
    *** source: 15326485-Fig-1b
  *** Smad2[Ab]IP phos(S250/S255)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: RGM1 in BMLS
    *** source: 15326485-Fig-1d
  *** Smad2[Ab]IP phos(S250/S255)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: RGM1 in BMLS "treated with PD169316"
    *** source: 15326485-Fig-4b
  *** Smad2[Ab]IP phos(S250/S255)[phosAb] is increased irt Tgfb1 (30 min)
    *** cells: RGM1 in BMLS "treated with SP600125"
    *** source: 15326485-Fig-4a
  *** Smad2[phosAb] phos(S245/S250/S255)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** repressed by: Ctdsp1 and/or Ctdsp2 [RNAi]
    *** source: 17085434-Fig-5b

Contradictions

  *** Smad2[phosAb] phos(S245/S250/S255)[phosAb] is unchanged irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** source: 17085434-Fig-1b
  *** xSmad2[phosAb] phos(S245/S250/S255)[phosAb] is unchanged irt TgfbR1 (1 hr)
    *** cells: HEK293 in BMS
    *** source: 17085434-Fig-2c
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is unchanged irt Tgfb1 (1 hr)
    *** cells: HEK293T<xSmad3> in BMS
    *** source: 17035229-Fig-2a
  ----- later times -----------------------------------------------------------
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is unchanged irt Tgfb1 (20 hr)
    *** cells: HACAT in BMS
    *** source: 17035229-Fig-7d
  *** xSmad2[tAb]IP phos(S465/S467)[phosAb] is unchanged irt Tgfb1 (20 hr)
    *** cells: HACAT in BMS
    *** unaffected by: xCtdsp1 [addition]
    *** source: 17035229-Fig-7d
  *** xSmad2[tAb]IP phos(T8)[phosAb] is unchanged irt Tgfb1 (20 hr)
    *** cells: HACAT in BMS
    *** source: 17035229-Fig-7d
  ----- not adjusted for protein relocations ------------------------------
  *** Smad2[phosAb] phos(S245/S250/S255)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS "cytosolic-extracts"
    *** source: 17085434-Fig-1d
  *** Smad2[phosAb] phos(S245/S250/S255)[phosAb] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS "nuclear-extracts"
    *** source: 17085434-Fig-1d




