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Myc protein expression is decreased itpo Fbxw7
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requires proteasome activity

  *** xMyc[Ab] prot-exp[WB] is decreased itpo xFbxw7
    *** cells: HEK293<xMyc> in BMS
    *** reversed by: MG132 [addition]
    *** source: 15150404-Fig-1c
  *** xMyc[Ab] prot-exp[WB] is decreased itpo xFbxw7b
    *** cells: HEK293<xMyc> in BMS
    *** reversed by: MG132 [addition]
    *** source: 15150404-Fig-1c
  *** xMyc[Ab] prot-exp[WB] is decreased itpo xFbxw7g
    *** cells: HEK293<xMyc> in BMS
    *** reversed by: MG132 [addition]
    *** source: 15150404-Fig-1c
  *** xMyc[Ab] prot-exp[WB] is decreased itpo xFbxw7g
    *** cells: HEK293<xMyc><xMax> in BMS
    *** reversed by: MG132 [addition]
    *** source: 15150404-Fig-2a

requires Fbxw7 F-box domain

  *** xMyc[Ab] prot-exp[WB] is decreased itpo xFbxw7
    *** cells: HEK293<xMyc> in BMS
    *** reversed by: xFbxw7(mnr)"delFbox" [substitution]
    *** source: 15150404-Fig-1a
  *** xMyc[Ab] prot-exp[WB] is decreased itpo xFbxw7b
    *** cells: HEK293<xMyc> in BMS
    *** reversed by: xFbxw7b(mnr)"delFbox" [substitution]
    *** source: 15150404-Fig-1a

is downstream of Gsk3a

  *** xMyc[Ab] prot-exp[WB] is decreased itpo xFbxw7g
    *** cells: HEK293<xMyc> in BMS
    *** comment: reversed by addition of the axin GSK-interacting domain
    *** reversed by: LiCl [addition]
    *** source: 15150404-Fig-3e
  *** xMyc[Ab] prot-exp[WB] is decreased itpo xFbxw7g
    *** cells: HEK293<xMyc> in BMS
    *** reversed by: xFbxw7g(mnr)"delFbox" [substitution]
    *** source: 15150404-Fig-1a

requires Myc T58 and T62 sites

  *** xMyc[Ab] prot-exp[WB] is decreased itpo xFbxw7g
    *** cells: HEK293<xMyc> in BMS
    *** reversed by: xMyc(T58A) [substitution]
    *** reversed by: xMyc(S62A) [substitution]
    *** reversed by: xMyc(T58A/S62A) [substitution]
    *** source: 15150404-Fig-3b

other

  *** xMyc[Ab] prot-exp[WB] is decreased itpo xFbxw7g
    *** cells: HEK293<xMyc><xMax> in BMS
    *** reversed by: xFbxw7g(R298H) [substitution]
    *** source: 15150404-Fig-1b
  *** xMyc[Ab] prot-exp[WB] is detectable
    *** cells: HEK293<xMyc> in BMS
    *** enhanced by: xFbxw7g(mnr)"WD" [addition]
    *** source: 15150404-Fig-3c
  *** Myc[Ab] prot-exp[WB] is detectable
    *** cells: HELA in BMS
    *** enhanced by: xFbxw7g(mnr)"WD" [addition]
    *** source: 15150404-Fig-4c
  *** Myc[Ab] prot-exp[WB] is increased itao Fbxw7 [RNAi]
    *** cells: HELA in BMS
    *** source: 15150404-Fig-4a
  *** Myc[Ab] prot-exp[WB] is increased itao Fbxw7 [RNAi]
    *** cells: hFibros in BMS
    *** source: 15150404-Fig-4a
  *** Myc[Ab] prot-exp[WB] is increased itao Fbxw7 [RNAi]
    *** cells: U2OS in BMS
    *** source: 15150404-Fig-4a
  *** Myc[Ab] prot-exp[WB] is increased itao Fbxw7 [KO]
    *** cells: mESCs in BMS
    *** source: 15103331-Fig-5a
  *** Myc[Ab] prot-exp[WB] is increased itao Fbxw7 [RNAi]
    *** cells: HELA in BMS
    *** source: 15103331-Fig-4b

===========================================
Myc protein stability is decreased by Fbxw7
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  *** xMyc[Ab] prot-stability[35S-pulse-chase] is decreased itpo xFbxw7g
    *** cells: HEK293<xMyc> in BMS
    *** source: 15150404-Fig-1d
  *** Myc[Ab] prot-stability[35S-pulse-chase] is increased itao Fbxw7 [RNAi]
    *** cells: HELA in BMS
    *** source: 15150404-Fig-4b
  *** Myc[Ab] prot-stability[CHX-WB] is detectable
    *** cells: HELA in BMS
    *** enhanced by: xFbxw7g(mnr)"WD" [addition]
    *** source: 15103331-Fig-4d
  *** xMyc[tAb]IP prot-stability[35S-pulse-chase] is decreased itpo xFbxw7
    *** cells: HEK293T in BMS
    *** reversed by: xFbxw7(mnr)"delFbox" [substitution]
    *** source: 15103331-Fig-2b
  *** xMyc[tAb]IP prot-stability[35S-pulse-chase] is decreased itpo xFbxw7
    *** cells: HEK293T in BMS
    *** reversed by: xMyc(T58A/S62A) [substitution]
    *** source: 15103331-Fig-3d
  *** xMyc[tAb]IP prot-stability[35S-pulse-chase] is increased itao Fbxw7 [KO]
    *** cells: HEK293T in BMS
    *** source: 15103331-Fig-5b
  *** xMyc[tAb]IP prot-stability[35S-pulse-chase] is increased itao Fbxw7 [RNAi]
    *** cells: HEK293T in BMS
    *** reversed by: xMyc(T58A/S62A) [substitution]
    *** source: 15103331-Fig-4c



