===================
Location in Nucleus
===================

requires Y710 phos site

  *** xStat1[tAb] locatedin(nucleus)[IHC] is increased irt Ifna (30 min)
    *** cells: HUH7<xStat1> in BMS
    *** inhibited by: xStat1(Y710A) [substitution]
    *** source: 11150296-Fig-5
  *** xStat1[tAb] locatedin(nucleus)[IHC] is increased irt Ifna (45 min)
    *** cells: HUH7<xStat1> in BMS "primed with Ifng for 24 hr"
    *** inhibited by: xStat1(Y710A) [substitution]
    *** source: 11150296-Fig-8a

===================
Location in Nucleus
===================

requires K410 and/or K413 acetyl sites

  *** xStat1[XFP] locatedin(nucleus)[IHC] is increased irt Ifna (times)
    *** cells: U3A<Stat1~null> in BMS
    *** times: 0 10+ 20++ 60++ min
    *** inhibited by: xStat1(K410Q) [substitution]
    *** inhibited by: xStat1(K413Q) [substitution]
    *** source: 19171783-Fig-2e
  *** xStat1[XFP] locatedin(nucleus)[IHC] is increased irt Ifna (times)
    *** cells: U3A<Stat1~null> in BMS
    *** times: 0 10+ 20++ 60++ min
    *** inhibited by: xStat1(K410Q/K413Q) [substitution]
    *** unaffected by: xStat1(K410R/K413R) [substitution]
    *** source: 19171783-Fig-1h
  *** xStat1[Ab] locatedin(nucleus)[IHC] is increased irt Ifna (times)
    *** cells: HELA in BMS
    *** times: 0 20 40 60+ min
    *** inhibited by: xStat1(K410Q/K413Q) [substitution]
    *** unaffected by: xStat1(K410R/K413R) [substitution]
    *** inhibited by: xStat1(K410Q) [substitution]
    *** inhibited by: xStat1(K413Q) [substitution]
    *** source: 19171783-Fig-S1f
  *** xStat1[Ab] locatedin(nucleus)[IHC] is increased irt Ifna (times)
    *** cells: HELA in BMS "treated with LMB"
    *** times: 0 1+ 2+ 3+ hr
    *** inhibited by: xStat1(K410Q/K413Q) [substitution]
    *** source: 19171783-Fig-S1f
  *** xStat1[tAb] locatedin(nucleus)[IHC] is increased irt Ifna (30 min)
    *** cells: HUH7<xStat1> in BMS
    *** inhibited by: xStat1(R378A/K379A) [substitution]
    *** inhibited by: xStat1(K410A/K413A) [substitution]
    *** inhibited by: xStat1(K413A/R418A) [substitution]
    *** source: 11150296-Fig-5
  *** xStat1[tAb] locatedin(nucleus)[IHC] is increased irt Ifna (45 min)
    *** cells: HUH7<xStat1> in BMS "primed with Ifng for 24 hr"
    *** inhibited by: xStat1(K410A/K413A) [substitution]
    *** source: 11150296-Fig-8a

  *** xStat1(K410R/K413R)[Ab] locatedin(nucleus)[IHC] is unchanged irt Ifna (24 hr)
    *** cells: U3A<Stat1~null> in BMS
    *** unaffected by: Rela [RNAi]
    *** source: 19171783-Fig-S3a
  *** xStat1(K410R/K413R)[XFP] locatedin(nucleus)[IHC] is unchanged irt Ifna (times)
    *** cells: HEK293T in BMS
    *** times: 0 10 20 30 60 min
    *** comment: relocation does occur in the absence of Ptpn2 [RNAi]
    *** source: 19171783-Fig-S3c
  *** xStat1(K410R/K413R)[XFP] locatedin(nucleus)[IHC] is unchanged irt Ifna (times)
    *** cells: U3A<Stat1~null> in BMS
    *** times: 0 10 20 30 60 min
    *** comment: relocation does occur in the absence of Ptpn2 [RNAi]
    *** source: 19171783-Fig-S3c
  *** xStat1[Ab] locatedin(nucleus)[IHC] is increased irt Ifna (1 hr)
    *** cells: U3A<Stat1~null><xStat1><Ptpn2~null> in BMS
    *** unaffected by: xStat1(K410Q/K413Q) [substitution]
    *** source: 19171783-Fig-6d
  *** xStat1[XFP] locatedin(nucleus)[IHC] is increased irt Ifna2 (30 min)
    *** cells: U3A<Stat1~null><xStat1> in BMS
    *** partially inhibited by: xStat1(L407A) [substitution]
    *** source: 11927559-Fig-5a


requires something to exit from nucleus first?

  *** Stat1[Ab] infraction(nuclear)[WB] is unchanged irt Ifna (5 min)
    *** cells: HEK293 in BMS "treated with LMB"
    *** source: 19171783-Fig-S1d

no extras

  *** Stat1[Ab] locatedin(nucleus)[IHC] is increased irt Ifna (45 min)
    *** cells: HUH7 in BMS "primed with Ifng for 24 hr"
    *** source: 11150296-Fig-3
  *** Stat1[Ab] locatedin(nucleus)[IHC] is increased irt Ifna (times)
    *** cells: HUH7 in BMS "primed with Ifng for 24 hr"
    *** times: 0+ 10++ 30+++ 60+++ 120++ 180+ min
    *** source: 11150296-Fig-1
  *** Stat1[Ab] infraction(nuclear)[WB] is increased irt Ifna (24 hr)
    *** cells: SKMEL37 in BMS
    *** comment: increase correlates with increase in Stat1 prot-exp
    *** source: 16481475-Fig-5b
  *** Stat1[Ab] locatedin(nucleus)[IHC] is increased irt Ifna (48 hr)
    *** cells: SKMEL37 in BMS
    *** source: 16481475-Fig-6c
  *** Stat1[Ab] locatedin(nucleus)[IHC] is increased irt Ifna (30 min)
    *** cells: FS2 in BMS
    *** source: 1496401-Fig-2
  *** Stat1[Ab] locatedin(nucleus)[IHC] is increased irt Ifna (30 min)
    *** cells: HELA in BMS
    *** source: 1496401-Fig-2


=============
Oligo-binding
=============

  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifnb1 (45 min)
    *** cells: T47D in BMS
    *** source: 7523403-Fig-1a
  *** Stat1{phos}[phosAb] snaggedby[WB] oligo[biotin] is increased irt Ifna (1 hr)
    *** cells: HEK293T in BMS
    *** oligo: GAS sequence-not-reported
    *** inhibited by: ValproicAcid [addition]
    *** source: 19171783-Fig-3g
  *** Stat1{phos}[phosAb] snaggedby[WB] oligo[biotin] is increased irt Ifna (1 hr)
    *** cells: HEK293T in BMS
    *** oligo: GAS sequence-not-reported
    *** inhibited by: xStat1(K410Q/K413Q) [addition]
    *** source: 19171783-Fig-3f
  *** xStat1{phos}[phosAb] snaggedby[WB] oligo[biotin] is increased irt Ifna (1 hr)
    *** cells: HEK293T in BMS "treated with Vanadate"
    *** oligo: GAS sequence-not-reported
    *** unaffected by: xStat1(K410Q/K413Q) [substitution]
    *** source: 19171783-Fig-S2d
  *** xStat1{phos}[phosAb] snaggedby[WB] oligo[biotin] is increased irt Ifna (1 hr)
    *** cells: U3A<Stat1~null> in BMS
    *** oligo: GAS sequence-not-reported
    *** source: 19171783-Fig-5f
  *** Stat1{phos}[phosAb] snaggedby[WB] oligo[biotin] is increased irt Ifna (1 hr)
    *** cells: HEK293 in BMS "treated with Vanadate for 30 min"
    *** oligo: GAS sequence-not-reported
    *** unaffected by: ValproicAcid [addition]
    *** source: 19171783-Fig-5g
  *** Stat1[Ab] oligo-binding[EMSA] is increased irt Ifna (15 min)
    *** cells: 2FTGH in BMS
    *** reqs: Stat2 [null-mut]
    *** source: 8621447-Fig-1a


==================
Protein Expression
==================

  *** Stat1[Ab] prot-exp[WB] is increased irt Ifna (24 hr)
    *** cells: HEK293 in BMS
    *** does not req: Shp2 [RNAi]
    *** does not req: Ptpn2 [RNAi]
    *** source: 19171783-Fig-6a
  *** xStat1[Ab] snaggedby[WB] oligo[biotin] is increased irt Ifna (1 hr)
    *** cells: U3A<Stat1~null><xStat1> in BMS
    *** oligo: GAS sequence-not-reported
    *** inhibited by: xStat1(K410Q) [substitution]
    *** inhibited by: xStat1(K413Q) [substitution]
    *** source: 19171783-Fig-2i
  *** xStat1[Ab] snaggedby[WB] oligo[biotin] is increased irt Ifna (1 hr)
    *** cells: U3A<Stat1~null><xStat1> in BMS
    *** oligo: GAS sequence-not-reported
    *** unaffected by: xStat1(K410R/K413R) [substitution]
    *** inhibited by: xStat1(K410Q/K413Q) [substitution]
    *** source: 19171783-Fig-2d
  *** Stat1[Ab] snaggedby[WB] oligo[biotin] is increased irt Ifna (15 min)
    *** cells: hPHAblasts in BMS
    *** oligo: "FcgR-GAS" 5-GTATTTCCCAGAAAAGGAAC-3
    *** source: 7988557-Fig-9b
  *** xStat1[Ab] snaggedby[WB] rKpna1[GST] is increased irt Ifna (1 hr)
    *** cells: U3A<Stat1~null> in BMS
    *** inhibited by: xStat1(K410Q/K413Q) [substitution]
    *** unaffected by: xStat1(K410R/K413R) [substitution]
    *** source: 19171783-Fig-1i

========================
acetyl on K410/K413
========================

  *** xStat1[Ab]IP acetyl[KAcAb] is increased irt Ifna (3 hr)
    *** cells: U3A<Stat1~null> in BMS
    *** inhibited by: xStat1(K410R/K413R) [substitution]
    *** source: 19171783-Fig-1m
  *** Stat1[Ab]IP acetyl[KAcAb] is increased irt Ifna (8 hr)
    *** cells: HEK293 in BMS
    *** comment: acetyl was lost after removal of Ifna for 3 hr
    *** source: 19171783-Fig-7f
  *** Stat1[Ab]IP acetyl[KAcAb] is increased irt Ifna (times)
    *** cells: HEK293 in BMS
    *** times: 0 5 60+ min
    *** source: 19171783-Fig-S1d
  *** Stat1[Ab]IP acetyl[KAcAb] is increased irt Ifna (times)
    *** cells: HEK293T in BMS
    *** times: 0 60+ 120+ 180+ min
    *** source: 19171783-Fig-1a
  *** Stat1[Ab]IP acetyl[KAcAb] is increased irt Ifna (times)
    *** cells: HELA in BMS
    *** times: 0 30+ 60+ min
    *** source: 17923090-Fig-S4a


===========
acetyl
===========

  *** xStat1[Ab] acetyl[KAcAb-ppt] is increased irt Ifna (3 hr)
    *** cells: U3A<Stat1~null> in BMS
    *** inhibited by: xStat1(K410R/K413R) [substitution]
    *** source: 19171783-Fig-1m
  *** Stat1[Ab] acetyl[KAcAb-ppt] is increased irt Ifna (48 hr)
    *** cells: SKMEL37 in BMS
    *** source: 16481475-Fig-6a
  *** Stat1[Ab]IP acetyl[KAcAb] is increased irt Ifna (48 hr)
    *** cells: SKMEL37 in BMS
    *** source: 16481475-Fig-6a

================
Coprecipitations
================

Cbp--

  *** Stat1[Ab] copptby[WB] Cbp[Ab] is increased irt Ifna (24 hr)
    *** cells: SKMEL37 in BMS
    *** comment: increase correlates with increase in Stat1 prot-exp
    *** source: 16481475-Fig-6c


Hdac1--

  *** Stat1[Ab] copptby[WB] Hdac1[Ab] is decreased irt Ifna (24 hr)
    *** cells: SKMEL37 in BMS
    *** source: 16481475-Fig-5f

Hdac2--

  *** Stat1[Ab] copptby[WB] Hdac3[Ab] is decreased irt Ifna (24 hr)
    *** cells: SKMEL37 in BMS
    *** source: 16481475-Fig-5f

Ptpn2--

  *** Stat1[Ab] colocwith[IHC] Ptpn2[Ab] is increased irt Ifna (times)
    *** cells: HELA in BMS
    *** times: 0 1+ 3 hr
    *** comment: colocalization takes place in the nucleus
    *** source: 19171783-Fig-7b
  *** Stat1[Ab] copptby[WB] xPtpn2(C216S/D182A)[Ab] is increased irt Ifna (times)
    *** cells: HEK293 in BMS "cytoplasmic-extracts"
    *** times: 0 60+ 120++ 180+++ 240+++ min
    *** source: 19171783-Fig-7e
  *** Stat1[Ab] copptby[WB] xPtpn2(C216S/D182A)[Ab] is increased irt Ifna (times)
    *** cells: HEK293 in BMS "nuclear-extracts"
    *** times: 0 5 20 40+ 60++ min
    *** source: 19171783-Fig-7e
  *** Stat1{acetyl}[KAcAb] copptby[WB] xPtpn2(C216S/D182A)[Ab] is increased irt Ifna (times)
    *** cells: HEK293 in BMS "cytoplasmic-extracts"
    *** times: 0 60+ 120++ 180+++ 240+++ min
    *** source: 19171783-Fig-7e
  *** Stat1{acetyl}[KAcAb] copptby[WB] xPtpn2(C216S/D182A)[Ab] is increased irt Ifna (times)
    *** cells: HEK293 in BMS "nuclear-extracts"
    *** times: 0 5 20 40+ 60++ min
    *** source: 19171783-Fig-7e

Pias1--

  *** Stat1[Ab] copptby[WB] Pias1[Ab] is increased irt Ifna (15 min)
    *** cells: DAUDI in BMS
    *** source: 9724754-Fig-4a
  *** Stat1[Ab] copptby[WB] Pias1[Ab] is increased irt Ifna (times)
    *** cells: DAUDI in BMS
    *** times: 0 5++ 15++ 30+ 60+ min
    *** source: 9724754-Fig-4b

not Pias2--

  *** Stat1[Ab] copptby[WB] Pias2[Ab] is unchanged irt Ifna (15 min)
    *** cells: hNKCs in BMS
    *** source: 12716907-Fig-1c
  *** Stat1[Ab] copptby[WB] xPtpn2(C216S/D182A)[Ab] is increased irt Ifna (times)
    *** cells: HEK293 in BMS
    *** times: 0 1+ 3++ hr
    *** source: 19171783-Fig-7c

Stat2--

  *** Stat1[Ab] copptby[WB] Stat2[Ab] is increased irt Ifna (15 min)
    *** cells: U266 in BMS
    *** source: 8647845-Fig-4a

