======================================
Proteins required for Ikba degradation
======================================

Erc1--

  *** Ikba[Ab] prot-exp[WB] is decreased irt Etoposide (2 hr)
    *** cells: HEK293 in BMS
    *** reqs: Erc1 [RNAi]
    *** source: 16497931-Fig-5a

Nemo--

  *** Ikba[Ab] prot-exp[WB] is decreased irt Etoposide (2 hr)
    *** cells: HEK293 in BMS
    *** reqs: Nemo [RNAi]
    *** source: 16497931-Fig-5a
  ------ requires phos site S85 or ubiquitination ------
  *** Ikba[Ab] prot-exp[WB] is decreased irt Etoposide (2 hr)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** reversed by: xNemo(S85A) [substitution]
    *** source: 16497931-Fig-1b
  *** Ikba[Ab] prot-exp[WB] is decreased irt Etoposide (2 hr)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** unaffected by: xNemo(ubiq/S85A) [substitution]
    *** source: 16497931-Fig-3b
  ------ requires Nemo sumo sites K277/K309 ------
  *** Ikba[Ab] prot-exp[WB] is decreased irt Etoposide (2 hr)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** inhibited by: xNemo(K277A/K309A) [substitution]
    *** source: 14651848-Fig-2e
  *** Ikba[Ab] prot-exp[WB] is decreased irt Etoposide (2 hr)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** partially reversed by: xNemo(K277A) [substitution]
    *** partially reversed by: xNemo(K309A) [substitution]
    *** source: 14651848-Fig-2d

not Ripk1--

  *** Ikba[Ab] prot-exp[WB] is decreased irt Etoposide (2 hr)
    *** cells: HEK293 in BMS
    *** does not req: Ripk1 [RNAi]
    *** source: 16497931-Fig-5a

not Rsk1--

  *** Ikba[Ab] prot-exp[WB] is decreased irt Etoposide (2 hr)
    *** cells: HEK293 in BMS
    *** does not req: Rsk1 [RNAi]
    *** source: 16497931-Fig-5a

Question: Ikba degradation is transient (5-30 min) irt Tnf.
Why is Ikba still gone 2 hrs after treatment with Etoposide?

  *** Ikba[Ab] prot-exp[WB] is unchanged irt Etoposide (2 hr)
    *** cells: HEK293 in BMS
    *** does not req: Atm [RNAi]
    *** source: 14651848-Fig-6a
  *** Ikba[Ab] prot-exp[WB] is unchanged irt Etoposide (2 hr)
    *** cells: HEK293 in BMS
    *** does not req: Atr [RNAi]
    *** source: 14651848-Fig-6a


=========================
Upregulation of Ikba-gene
=========================

  *** Ikba-gene mRNA[RTPCR] is increased irt Etoposide (2 hr)
    *** cells: 70Z3<Nemo~null><xNemo> in BMS
    *** inhibited by: xNemo(S85A) [substitution]
    *** source: 16497931-Fig-1e

