========
Activity
========

  *** xSmad2(1-467)[Gal4-DBD] Gal4-reporter[Luc] is detectable
    *** cells: HEPG2<xSmad2> in BMS
    *** inhibited by: xSmad2(S464A) [substitution]
    *** inhibited by: xSmad2(S465A) [substitution]
    *** inhibited by: xSmad2(S467A) [substitution]
    *** inhibited by: xSmad2(S464A/S465A/S467A) [substitution]
    *** source: 9346908-Fig-7
  *** rSmad2(mnr)"MH1-domain" oligo-binding[EMSA] is undetectable
    *** cells: none
    *** oligo: "Myc-TIE" 5-TTCTCAGAGGCTTGGCGGGAAAAAGAACGG-3
    *** source: 14993291-Fig-4a
  *** rSmad2(mnr)"MH1-domain" oligo-binding[EMSA] is undetectable
    *** cells: none
    *** oligo: SBE 5-AGTATGTCTAGAC TGA-3
    *** source: 14993291-Fig-4a
  *** rSmad3 oligo-binding[EMSA] is detectable
    *** cells: none
    *** oligo: AP1 5-CGCTTGATGAGTCAGCCGGAA-3
    *** inhibited by: rSmad3(1-144)"N-term" [substitution]
    *** enhanced by: rSmad3(1-210)"LN-term" [substitution]
    *** inhibited by: rSmad3(210-425)"C-term" [substitution]
    *** inhibited by: rSmad3(144-425)"LC-term" [substitution]
    *** source: 9732876-Fig-3a
  *** rSmad3 oligo-binding[EMSA] is detectable
    *** cells: none
    *** oligo: AP1 5-CGCTTGATGAGTCAGCCGGAA-3
    *** unaffected by: rJun [addition]
    *** source: 9732876-Fig-3c
  *** rSmad3 oligo-binding[EMSA] is detectable
    *** cells: none
    *** oligo: PAI1-PE21 5-CCTAGACAGACAAAACCTAGACAATCACGTG-3
    *** comment: details of oligo sequence requirements not curated
    *** comment: rSmad3 can form a complex with rSmad4 and rTfe3 at this site
    *** source: 12551947-Fig-1a,1b,1c

===============
Phosphorylation
===============

phos(T8)

  *** Smad2[Ab]IP phos(T8)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdspL2 [addition]
    *** source: 17035229-Fig-5c
  *** xSmad2[tAb]IP phos(T8)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xPpm1a [addition]
    *** source: 17035229-Fig-2a
  *** xSmad2[tAb]IP phos(T8)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdp1a [addition]
    *** source: 17035229-Fig-5b
  *** xSmad2[tAb]IP phos(T8)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdspL2 [addition]
    *** source: 17035229-Fig-5a
  *** xSmad2[tAb]IP phos(T8)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xPpp1ca [addition]
    *** unaffected by: xPpp1cc [addition]
    *** unaffected by: xPpp2ca [addition]
    *** unaffected by: xPpp2cb [addition]
    *** unaffected by: xPpp3ca [addition]
    *** source: 17035229-Fig-1c

phos(T179)

  *** Smad2[Ab]IP phos(T179)[phosAb] is detectable
    *** cells: HACAT in BMS
    *** does not req: Ctdsp1 and/or Ctdsp2 and/or CtdspL [RNAi]
    *** source: 17035229-Fig-5d

phos(S208)

  *** Smad2[Ab]IP phos(S208)[phosAb] is detectable
    *** cells: HACAT in BMS
    *** does not req: Ctdsp1 and/or Ctdsp2 and/or CtdspL [RNAi]
    *** source: 17035229-Fig-5d

phos(T220)

  *** Smad2[Ab]IP phos(T220)[phosAb] is detectable
    *** cells: HACAT in BMS
    *** does not req: Ctdsp1 and/or Ctdsp2 and/or CtdspL [RNAi]
    *** source: 17035229-Fig-5d
  *** xSmad2[tAb]IP phos(T220)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xPpm1a [addition]
    *** source: 17035229-Fig-2a
  *** xSmad2[tAb]IP phos(T220)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdp1a [addition]
    *** source: 17035229-Fig-5b
  *** xSmad2[tAb]IP phos(T220)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdspL2 [addition]
    *** source: 17035229-Fig-5a
  *** xSmad2[tAb]IP phos(T220)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xPpp1ca [addition]
    *** unaffected by: xPpp1cc [addition]
    *** unaffected by: xPpp2ca [addition]
    *** unaffected by: xPpp2cb [addition]
    *** unaffected by: xPpp3ca [addition]
    *** source: 17035229-Fig-1c

phos(S245/S250/S255)

  *** Smad2[Ab]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HACAT in BMS
    *** does not req: Ctdsp1 and/or Ctdsp2 and/or CtdspL [RNAi]
    *** source: 17035229-Fig-5d
  *** Smad2[Ab]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdspL2 [addition]
    *** source: 17035229-Fig-5c
  *** xSmad2[phosAb] phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293 in BMS
    *** source: 17085434-Fig-2c
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xPpm1a [addition]
    *** source: 17035229-Fig-2a
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdp1a [addition]
    *** source: 17035229-Fig-5b
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdspL2 [addition]
    *** source: 17035229-Fig-5a
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xPpp1ca [addition]
    *** unaffected by: xPpp1cc [addition]
    *** unaffected by: xPpp2ca [addition]
    *** unaffected by: xPpp2cb [addition]
    *** unaffected by: xPpp3ca [addition]
    *** source: 17035229-Fig-1c

phos(S465/S467)

  *** xSmad2[tAb]IP phos(S465/S467)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xPpm1a [addition]
    *** source: 17035229-Fig-2a

phos(sitenr)

  *** Smad2[phosAb] phos(sitenr)[phosAb] is detectable
    *** cells: BXPC3<Smad4~null> in BMS "treated with Tgfb1 (tnr)" "cytoplasmic-extracts"
    *** enhanced by: PD98059 [addition]
    *** source: 17638924-Fig-2b
  *** Smad2[phosAb] phos(sitenr)[phosAb] is detectable
    *** cells: BXPC3<Smad4~null> in BMS "treated with Tgfb1 (tnr)" "nuclear-extracts"
    *** enhanced by: PD98059 [addition]
    *** source: 17638924-Fig-2b
  *** Smad2[phosAb] phos(sitenr)[phosAb] is detectable
    *** cells: CAPAN1<Smad4~null> in BMS "treated with Tgfb1 (tnr)" "cytoplasmic-extracts"
    *** enhanced by: xKras(mnr)"DN" [addition]
    *** enhanced by: PD98059 [addition]
    *** source: 17638924-Fig-2a
  *** Smad2[phosAb] phos(sitenr)[phosAb] is detectable
    *** cells: CAPAN1<Smad4~null> in BMS "treated with Tgfb1 (tnr)" "nuclear-extracts"
    *** enhanced by: xKras(mnr)"DN" [addition]
    *** enhanced by: PD98059 [addition]
    *** source: 17638924-Fig-2a

total phos

  *** xSmad2[tAb]IP phos[32Pi] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xSmad2(S464A) [substitution]
    *** enhanced by: xSmad2(S465A) [substitution]
    *** enhanced by: xSmad2(S467A) [substitution]
    *** enhanced by: xSmad2(S464A/S465A/S467A) [substitution]
    *** source: 9346908-Fig-4
  *** xSmad2[tAb]IP phos[32Pi] is detectable
    *** cells: NIH3T3 in BMS
    *** inhibited by: xCav1 [addition]
    *** source: 11102446(D)
  *** xSmad2[tAb]IP phos[32Pi] is detectable
    *** cells: R1BL17<TgfbR1~null> in BMS
    *** inhibited by: xSmad2(S464A/S465A/S467A) [substitution]
    *** source: 10197981-Fig-6a
  *** xSmad2[tAb]IP phos[32Pi] is detectable
    *** cells: R1BL17<TgfbR1~null> in BMS
    *** inhibited by: xSmad2(T220V/S245A/S250A/S255A) [substitution]
    *** source: 10197981-Fig-5b

===================
Ubiquitination ????
===================

  *** xSmad2(mnr)"MH2"[tAb]IP upshift[WBMS] is detectable
    *** cells: COS1 in BMS
    *** comment: enhanced by addition of an NLS to xSmad2 C-term
    *** source: 10587642-Fig-5d



=====================
Location requirements
=====================

nuclear export--

  *** xSmad2(185-467)"NLS-fused-LMH2"[tAb] nuc-export[HeterokaryonAssay] is detectable
    *** cells: GSN2 in BMS
    *** source: 12191473-Fig-4b

nuclear import--

  *** rSmad2(186-467)"LMH2"[Ab] nuc-import[IVnucImport] is detectable
    *** cells: none
    *** inhibited by: rSmad2(1-185)"MH1" [substitution]
    *** comment: does not require cytosol
    *** source: 12917407-Fig-2a
  *** rSmad2(186-467)"LMH2"[tAb] nuc-import[IVnucImport] is detectable
    *** cells: none
    *** unaffected by: rRan(Q69L){GTP} [addition]
    *** comment: does not require cytosol
    *** source: 12917407-Fig-4

cytoplasm--

  *** Smad2{phos(S250/S255)}[phosAb] locatedin(cytoplasm)[IHC] is detectable
    *** cells: RGM1 in BMLS "treated with Hgf for 60 min"
    *** comment: phos signal is inhibited by SP600125"
    *** source: 15326485-Fig-6a,6b
  *** Smad2{phos(S250/S255)}[phosAb] locatedin(cytoplasm)[IHC] is detectable
    *** cells: RGM1 in BMLS "treated with Tgfb1 for 60 min"
    *** comment: phos signal is inhibited by SP600125"
    *** source: 15326485-Fig-6a,6b
  *** xSmad2[tAb] infraction(cytoplasmic)[WB] is detectable
    *** cells: COS1 in BMS
    *** comment: xSmad2 in nuclear fraction increases when fused to an NLS at the N-term
    *** source: 10587642-Fig-5c

nucleus-cytoplasm--

  *** Smad2[Ab] locatedin(nucleus-cytoplasm)[IHC] is detectable
    *** cells: MV1LU in BMS
    *** source: 9865696-Fig-4b
  *** xSmad2[tAb] locatedin(nucleus-cytoplasm)[IHC] is detectable
    *** cells: MV1LU in BMS
    *** source: 9865696-Fig-4b

nucleus--

  *** xSmad2(185-467)"LMH2"[tAb] locatedin(nucleus)[IHC] is detectable
    *** cells: HELA in BMS "treated with Tgfb1 for 30 min"
    *** comment: substitution with xSmad2((185-467)/C380R/L382R) causes a perinuclear pattern
    *** source: 12191473-Fig-2f
  *** xSmad2(185-467)"LMH2"[tAb] locatedin(nucleus)[IHC] is detectable
    *** cells: HELA in BMS "treated with Tgfb1 for 30 min"
    *** inhibited by: xSmad2((185-467)/V398R) [substitution]
    *** unaffected by: xSmad2((185-467)/P445H) [substitution]
    *** inhibited by: xSmad2((185-467)/W448A) [substitution]
    *** inhibited by: xSmad2((185-467)/L382R/K383A) [substitution]
    *** source: 12191473-Fig-2f
  *** Smad2[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: HELA in BMS "treated with Tgfb1 for 30 min"
    *** inhibited by: xNup214(1864-2091)"DN" [addition]
    *** source: 12191473-Fig-2a
  *** Smad2[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: HELA in BMS "treated with Tgfb1 for 30 min"
    *** partially inhibited by: xNup214(1805-2091)"DN" [addition]
    *** inhibited by: rKpnb1 [addition]
    *** source: 12191473-Fig-2b
  *** Smad2{phos(S465/S467)}[phosAb] locatedin(nucleus)[IHC] is detectable
    *** cells: RGM1 in BMLS "treated with Hgf for 60 min"
    *** comment: phos signal is inhibited by SP600125"
    *** source: 15326485-Fig-6a,6b
  *** Smad2{phos(S465/S467)}[phosAb] locatedin(nucleus)[IHC] is detectable
    *** cells: RGM1 in BMLS "treated with Tgfb1 for 60 min"
    *** comment: phos signal is unaffected by SP600125"
    *** source: 15326485-Fig-6a,6b
  *** xSmad2(mnr)"MH2"[tAb] infraction(nuclear)[WB] is detectable
    *** cells: COS1 in BMS
    *** comment: xSmad2(mnr)"MH2" in nuclear fraction increases when fused to an NLS at the N-term
    *** source: 10587642-Fig-5c

================================
Interactions with other Proteins
================================

Nup98--

  *** Smad2[Ab] copptby[WB] xNup98[tAb] is undetectable
    *** cells: COS1 in BMS
    *** source: 12191473-Fig-1b

Nup153--

  *** xSmad2[tAb]IP boundby[WB] rNup153(1156-1476)[MalBP] is detectable
    *** cells: none
    *** unaffected by: xSmad2{phos(S465/S467)} [substitution]
    *** comment: experimental details were not available so these are a guess
    *** source: 12191473-Fig-7a

Nup214--

  *** Smad2[Ab] copptby[WB] xNup214[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 12191473-Fig-1b
  *** rSmad2[tAb] boundby[WB] rNup214(1805-2091)[tAb] is detectable
    *** cells: none
    *** inhibited by: rKpnb1 [addition]
    *** source: 12191473-Fig-2c
  *** xSmad2[tAb] snaggedby[WB] rNup214(1805-2091)[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 12917407-Fig-6b
  *** xSmad2[tAb]IP boundby[WB] rNup214(1805-2091)[MalBP] is detectable
    *** cells: none
    *** unaffected by: xSmad2{phos(S465/S467)} [substitution]
    *** comment: experimental details were not available so these are a guess
    *** source: 12191473-Fig-7a

Pdpk1--

  *** Smad2[Ab] copptby[WB] Pdpk1[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17327236-Fig-1c
  *** xSmad2[tAb] copptby[WB] Pdpk1[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17327236-Fig-1b
  *** xSmad2[tAb] copptby[WB] xPdpk1[GST] is unchanged irt Ins (20 min)
    *** cells: HEK293T in BMS
    *** unaffected by: Wortmannin [addition] no-bkg-control
    *** source: 17327236-Fig-3b
  *** xSmad2[tAb] copptby[WB] xPdpk1[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 17327236-Fig-1a

Pmlc--

  *** xSmad2[tAb] copptby[WB] xPmlc[tAb] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: Tgfb1 (1 hr) [stim]
    *** source: 15356634-Fig-2b

Pias4--

  *** xSmad2[tAb] copptby[WB] xPias4[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 12904571-Fig-1b

Sara--

  *** Smad2[Ab] copptby[WB] Sara[Ab] is detectable
    *** cells: HEPG2 in BMS
    *** source: 9865696-Fig-2c
  *** xSmad2[tAb] copptby[WB] xSara[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 17272273-Fig-4a
  *** xSmad2[tAb]IP boundby[WB] rSara(mnr)"SBD"[GST] is detectable
    *** cells: none
    *** inhibited by: xSmad2{phos(S465/S467)} [substitution]
    *** comment: experimental details were not available so these are a guess
    *** source: 12191473-Fig-7a
  *** xSmad2[tAb] snaggedby[WB] xSara(665-750)"SBD"[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 9865696-Fig-6b
  *** xSmad2[tAb] snaggedby[WB] xSara(665-750)"SBD"[tAb] is detectable
    *** cells: COS1 in BMS
    *** unaffected by: xTgfbR1 [addition]
    *** inhibited by: xTgfbR1(T204D)"CA" [addition]
    *** source: 9865696-Fig-6c

Smad4--

  *** rSmad2[35S] boundby[WB] rSmad4[GST] is detectable
    *** cells: none
    *** partially inhibited by: rSmad2(T8D/T220D/S245D/S250D/S255D) [substitution]
    *** enhanced by: rSmad2(S465E/S467E) [substitution]
    *** enhanced by: rSmad2(T8D/T220D/S245D/S250D/S255D/S465E/S467E) [substitution]
    *** source: 12193595-Fig-6b

Smurf1--

  *** xSmad2[tAb] copptby[WB] xSmurf1(C710A)"CIA"[tAb] is undetectable
    *** cells: COS7 in BMS
    *** source: 11278251-Fig-1b

Sptbn1--

  *** Smad2[Ab] copptby[WB] Sptbn1[Ab] is undetectable
    *** cells: HEPG2 in BMS
    *** unaffected by: TgfbR1 (1 hr) [stim]
    *** source: 12543979-Fig-2

Strap1--

  *** xSmad2[tAb] copptby[WB] xStrap[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 10757800(D)

Zfyve16--

  *** xSmad2[tAb] copptby[WB] xZfyve16[tAb] is undetectable
    *** cells: HEK293T in BMS
    *** source: 17272273-Fig-4a
