===============================
direct phosphorylation by Erks
===============================

Total

  *** rFos[32P] phos[32P-ATP] is increased by Erks{act}[Ab]IP
    *** cells: none
    *** IPfrom: NIH3T3 in BMLS "treated with serum (5 min)"
    *** comment: phos site was determined to be S374 by tryptic peptide mapping
    *** source: 8248197-Fig-1a
  *** rFos(209-380)[Ab] phos[WBMS] is increased by rErks{act}
    *** cells: none
    *** source: 12972619-Fig-7c
  *** rFos(209-380)[pSTAb] STphos[pSTAb] is increased by rErks{act}
    *** cells: none
    *** source: 12972619-Fig-7c
  *** rFos[32P] phos[32P-ATP] is increased by rErk2
    *** cells: none
    *** source: 16051177-Fig-1a

on S362 S374

  *** rFos(S362E/S374E)[32P] phos[32P-ATP] is increased by rErk2
    *** cells: none
    *** inhibited by: rFos(S362E/S374E/F343A) [substitution]
    *** inhibited by: rFos(S362E/S374E/Y345A) [substitution]
    *** source: 12134156-Fig-3a
  *** rFos(S362E/S374E)[32P] phos[32P-ATP] is increased by rErk2
    *** cells: none
    *** inhibited by: rFos(S362E/S374E/T325A) [substitution]
    *** inhibited by: rFos(S362E/S374E/T325A/T331A) [substitution]
    *** source: 12134156-Fig-4a
  *** rFos[32P] phos[32P-ATP] is increased by Erk1[Ab]IP
    *** cells: none
    *** comment: Erk1[Ab]IP is from NIH3T3 treated with Egf for 5 min
    *** partially inhibited by: rFos(S362E/S374E) [substitution]
    *** partially inhibited by: rFos(S362A/S374A) [substitution]
    *** source: 12134156-Fig-2d

on T232 T325 T331 S374

  *** rFos(209-380)[32P] phos[32P-ATP] is increased by xErk2{act}[tAb]IP
    *** cells: none
    *** IPfrom: HEK293T<xMek1(mnr)"CA"> in BMS
    *** inhibited by: rFos((209-380)/T232A/T325A/T331A/S374A) [substitution]
    *** source: 12972619-Fig-7a
  *** rFos(209-380)[32P] phos[32P-ATP] is increased by rErks{act}
    *** cells: none
    *** inhibited by: rFos((209-380)/T232A/T325A/T331A/S374A) [substitution]
    *** inhibited by: rFos((209-380)/T232A) [substitution]
    *** partially inhibited by: rFos((209-380)/T325A) [substitution]
    *** partially inhibited by: rFos((209-380)/T331A) [substitution]
    *** partially inhibited by: rFos((209-380)/T374A) [substitution]
    *** source: 12972619-Fig-8a
  *** rFos(209-380)[32P] phos[32P-ATP] is increased by rErks{act}
    *** cells: none
    *** inhibited by: rFos((209-380)/T232A/T325A/T331A/S374A) [substitution]
    *** source: 12972619-Fig-7b
  *** rFos(210-308)[32P] phos[32P-ATP] is increased by pErk2
    *** cells: none
    *** inhibited by: xFos((210-308)/T232A) [substitution]
    *** source: 7816602-Fig-4

======================================================
phosphorylation in the presence of overexpressed Erks
======================================================

  *** Fos[phosAb] phos(T325)[phosAb] is increased itpo xErk2
    *** cells: HELA in BMS
    *** partially inhibited by: xErk2(L232A) [substitution]
    *** inhibited by: xErk2(Y261A) [substitution]
    *** unaffected by: xErk2(D319N) [substitution]
    *** source: 16051177-Fig-S1c

===============================================
activation requires phos on T232 T325 T331 S374
===============================================

  *** xFos(TAD)[Gal4-DBD] Gal4-reporter[Luc] is increased itpo (xErk2 + xMek1(mnr)"CA")
    *** cells: NIH3T3 in BMLS
    *** inhibited by: xFos((209-380)/T232A/T325A/T331A/S374A) [substitution]
    *** partially inhibited by: xFos((209-380)/T232A) [substitution]
    *** partially inhibited by: xFos((209-380)/T325A) [substitution]
    *** partially inhibited by: xFos((209-380)/T331A) [substitution]
    *** partially inhibited by: xFos((209-380)/T374A) [substitution]
    *** source: 12972619-Fig-5b
  *** xFos(TAD)[Gal4-DBD] Gal4-reporter[Luc] is increased itpo (xErk2 + xMek1(mnr)"CA")
    *** cells: NIH3T3 in BMLS
    *** inhibited by: xFos((209-380)/T232A/T325A/T331A/S374A) [substitution]
    *** source: 12972619-Fig-5b
  *** xFos[Ab] phos[WBMS-PPase] is increased itpo (xErk2 + xMek1(mnr)"CA")
    *** cells: NIH3T3 in BMLS
    *** inhibited by: xFos(T232A/T325A/T331A/S374A) [substitution]
    *** source: 12972619-Fig-6b
  *** xFos(210-308)[Gal4-DBD] Gal4-reporter[CAT] is increased itpo xHras(G12V)
    *** cells: 1BR in BMS
    *** inhibited by: xFos((210-308)/T232A) [substitution]
    *** source: 7816602-Fig-3

  *** xFos(TAD)[Gal4-DBD] Gal4-reporter[Luc] is increased itpo (xErk2 + xMek1(mnr)"CA")
    *** cells: NIH3T3 in BMLS
    *** source: 12972619-Fig-4a
  *** xFos[Ab] phos[WBMS] is increased itpo (xErk2 + xMek1(mnr)"CA")
    *** cells: HEK293T in BMLS
    *** source: 12972619-Fig-7d
  *** Fos[Ab] phos[WBMS] is increased itpo (xErk2 + xMek1(mnr)"CA")
    *** cells: NIH3T3 in BMLS
    *** source: 12972619-Fig-4c
  *** xFos[Ab]IP STphos[pSTAb] is increased itpo (xErk2 + xMek1(mnr)"CA")
    *** cells: HEK293T in BMLS
    *** source: 12972619-Fig-7d
  *** xFos[Gal4-DBD] Gal4-reporter[Luc] is increased itpo (xErk2 + xMek1(mnr)"CA")
    *** cells: NIH3T3 in BMLS
    *** source: 12972619-Fig-4a
  *** xFos(TAD)[Gal4-DBD] Gal4-reporter[Luc] is increased itpo (xErk2 + xMek1(mnr)"CA")
    *** cells: NIH3T3 in BMLS
    *** source: 16123044-Fig-4a
