=================
Required Proteins
=================

not Mekk1--

  *** Traf2[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS "P100 fraction"
    *** times: 0 10+ 30 min
    *** unaffected by: kMekk1(1-1188)"del-kinase-domain" [addition]
    *** source: 18635759-Fig-4a

not Nemo--

  *** Traf2[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: 70Z3 in BMS "P100 fraction"
    *** times: 0 10+ 30 min
    *** does not req: Nemo [KO]
    *** source: 18635759-Fig-4b

not Ubc13--

  *** Traf2[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS "P100 fraction"
    *** times: 0 10+ 30 min
    *** does not req: Ubc13 [RNAi]
    *** source: 18635759-Fig-4c

not Traf3--

  *** Traf2[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS "P100-fraction"
    *** times: 0 5+ 10+ 30 min
    *** does not req: Traf3 [RNAi]
    *** source: 18635759-Fig-S12

No extras

  *** Traf2[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0+ 10++ 30+ min
    *** source: 18635759-Fig-2c
  *** Traf2[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS "P100 fraction"
    *** times: 0 10++ 30+ min
    *** source: 18635759-Fig-3a

======================
Fractionation evidence
======================

  *** Traf2[Ab] infraction(P100)[WB] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 10+ 30+ 60 min
    *** source: 18635759-Fig-S6
  *** Traf2[Ab] infraction(DRM)[WB] is increased irt anti-Cd40 (10 min)
    *** cells: M1241<xCd40> in BMS
    *** inhibited by: xCd40(mnr)"del-55" [substitution]
    *** source: 10748139-Fig-2a
  *** Traf2[Ab] infraction(DRM)[WB] is increased irt anti-Cd40 (10 min)
    *** cells: M1241<xCd40> in BMS
    *** source: 10748139-Fig-6
  *** Traf2[Ab] infraction(DRM)[WB] is increased irt anti-Cd40 (times)
    *** cells: M1241 in BMS
    *** times: 0 1+ 2+ 5+ 10+ 30+ 60+ min
    *** source: 10748139-Fig-2c

====================
Immunohistochemistry
====================

  *** xTraf2[XFP] locatedin(cellmembrane)[IHC] is increased irt anti-Cd40 (10 min)
    *** cells: M1241 in BMS
    *** comment: if treatment is performed at 370C Traf7 also appears in endosomes
    *** source: 10748139-Fig-1
