======================
Direct Phosphorylation
======================

phos(S32A/S36A)

  *** rIkba[32P] phos[32P-ATP] is increased by rIkk2
    *** cells: none
    *** inhibited by: rIkba(S32A/S36A)"phos-sites" [substitution]
    *** source: 14679297-Fig-1a
  *** rIkba(1-54)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: COS in BMS
    *** inhibited by: rIkba((1-54)/S32A/S36A) [substitution]
    *** source: 10779355-Fig-7c
  *** rIkba[32P] phos[32P-ATP] is increased by rIkk2
    *** cells: none
    *** unaffected by: rIkba(D31A) [substitution]
    *** partially inhibited by: rIkba(D31A/S32A) [substitution]
    *** partially inhibited by: rIkba(D31A/S36A) [substitution]
    *** inhibited by: rIkba(S32A/S36A) [substitution]
    *** source: 10514433-Fig-2b
  *** rIkba[32P] phos[32P-ATP] is increased by rIkk2
    *** cells: none
    *** unaffected by: rIkba(D31A) [substitution]
    *** unaffected by: rIkba(D31E) [substitution]
    *** unaffected by: rIkba(G33A) [substitution]
    *** inhibited by: rIkba(S32A/S36A) [substitution]
    *** source: 10514433-Fig-2a
  *** rIkba[32P] phos[32P-ATP] is increased by rIkk2
    *** cells: none
    *** inhibited by: rIkba(S32A/S36A) [substitution]
    *** source: 9689078-Fig-1f
  *** rIkba[32P] phos[32P-ATP] is increased by rIkk2
    *** cells: none
    *** inhibited by: rIkba(S32A/S36A) [substitution]
    *** source: 11158290-Fig-1b

Total

  *** rIkba[32P] phos[32P-ATP] is increased by rIkk2
    *** cells: none
    *** source: 11520989-Fig-4b
  *** rIkba[32P] phos[32P-ATP] is increased by rIkk2
    *** cells: none
    *** unaffected by: rNkiras1 [addition]
    *** source: 15024091-Fig-2a
  *** rIkba(1-54)[32P] phos[32P-ATP] is increased by rIkk2(mnr)"CA"
    *** cells: none
    *** source: 21765415-Fig-1f

==========================
SemiDirect Phosphorylation
==========================

  *** rIkba[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** source: 17693255-Fig-S7a
  *** rIkba(1-54)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: COS in BMS
    *** unaffected by: xIkk2(S177E/S181E)[tAb]IP [substitution]
    *** inhibited by: xIkk2(del(560-744)/K44M)[tAb]IP [substitution]
    *** inhibited by: xIkk2(S177A/S181A)[tAb]IP [substitution]
    *** inhibited by: xIkk2(K44M)[tAb]IP [substitution]
    *** source: 10779355-Fig-7c
  *** rIkba(1-54)[32P] phos[32P-ATP] is increased by Nemo[Ab]IP
    *** cells: none
    *** IPfrom: mEFs in BMS "treated with Tnf for 20 min"
    *** does not req: Ikk1 [KO]
    *** source: 11733537-Fig-4b
  *** rIkba(1-54)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HELA<xIkk2> in BMS "treated with Tnf for 10 min"
    *** inhibited by: rIkba((1-54)/S32A/S36A) [substitution]
    *** source: 9346241-Fig-2a
  *** rIkba[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HELA<xIkk2> in BMS "treated with Tnf for 10 min"
    *** inhibited by: rIkba(S32A/S36A) [substitution]
    *** unaffected by: rIkba(S32A) [substitution]
    *** unaffected by: rIkba(S36A) [substitution]
    *** source: 9346241-Fig-2a
  *** rIkba(1-100)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** inhibited by: rIkba(1-100/S32A/S36A) [substitution]
    *** source: 9520401-Fig-3a
  *** rIkba(1-100)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** inhibited by: xIkk2(K44A)"KD" [substitution]
    *** source: 9520401-Fig-3a
  *** rIkba(1-100)[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xIkk2> in BMS
    *** source: 9520401-Fig-3b
  *** rIkba[32P] phos[32P-ATP] is increased by xIkk2[tAb]IP
    *** cells: none
    *** IPfrom: HELA<xIkk2> in BMS
    *** comment: enhanced by treatment of cells with Tnf for 5 min
    *** source: 10469655-Fig-4b

=================================
Coprecipitation with active Ikk2
=================================

  *** Ikba[Ab] copptby[WB] xIkk2(S177E/S181E)[tAb] is detectable
    *** cells: mEFs<Ikk2~null> in BMS "on gelatin"
    *** inhibited by: xIkk2(WT) [substitution]
    *** inhibited by: xIkk2(K44M) [substitution]
    *** inhibited by: xIkk2(K44M/S177A/S181E) [substitution]
    *** source: 14636585-Fig-4c

