=================
Direct Activation
=================

  *** rErk1 IVKA(MBP)[32P-ATP] is increased by rMek1
    *** cells: none
    *** source: 8226933-Fig-1a
  *** rErk2 IVKA(MBP)[32P-ATP] is increased by rMek1
    *** cells: none
    *** source: 8226933-Fig-1b
  *** rErk2 IVKA(MBP)[32P-ATP] is increased by rMek1(mnr)"CA"
    *** cells: none
    *** source: 9166961-Fig-2

======================
Direct Phosphorylation
======================

  *** rErk2[32P] phos[32P-ATP] is increased by rMek1(S218D/S222D)"CA"
    *** cells: none
    *** source: 12167697-Fig-3a
  *** rErk2(mnr)"KD"[32P] phos[32P-ATP] is increased by rMek1(mnr)"CA"
    *** cells: none
    *** source: 11134045(D)
  *** rErk2(K52R)[32P] phos[32P-ATP] is unchanged by rMek1
    *** cells: none
    *** unaffected by: rTaok2(1-320)"CA" [addition]
    *** source: 10497253-Fig-2a
  *** rErk2[phosAb] phos(TEY)[phosAb] is increased by rMek1(mnr)"CA"
    *** cells: none
    *** source: 9166961-Fig-3
  *** rErk2[phosAb] phos(TEY)[phosAb] is increased by rMek1(mnr)"CA"
    *** cells: none
    *** source: 9166961-Fig-4
  *** rErk2[32P] phos[32P-ATP] is increased by rMek1(mnr)"CA"
    *** cells: none
    *** inhibited by: rPea15 [addition]
    *** source: 14707138-Fig-2

==================
Direct Interaction
==================

  *** rErk1[35S] boundby[WB] rMek1[GST] is detectable
    *** cells: none
    *** source: 11134045(D)
  *** rErk2[35S] boundby[WB] rMek1[GST] is detectable
    *** cells: none
    *** source: 11134045(D)

==================================
Activation in the presence of Mek1
==================================

reqs Pkcz--

  *** xErk1[tAb]IP IVKA(Elk1)[32P-ATP] is increased itpo xMek1(S218E/S222E/del(32-51))"CA"
    *** cells: HC11 in BMS
    *** inhibited by: xPkcz(K275W)"DN" [addition]
    *** source: 11551901-Fig-4a

not Pkce--

  *** xErk1[tAb]IP IVKA(Elk1)[32P-ATP] is increased itpo xMek1(S218E/S222E/del(32-51))"CA"
    *** cells: HC11 in BMS
    *** unaffected by: xPkce(K436R)"DN" [addition]
    *** source: 11551901-Fig-4a

not Shoc2--

  *** xErk1[tAb]IP IVKA(Elk1)[32P-ATP] is increased itpo xMek1(mnr)"CA"
    *** cells: HEK293 in BMLS
    *** unaffected by: xShoc2 [addition]
    *** source: 10783161-Fig-2a

Mek1 required features

  *** Erk2[Ab]IP IVKA(MBP)[32P-ATP] is increased itpo xMek1(mnr)"CA"
    *** cells: HEK293 in BMS
    *** inhibited by: xMek1(mnr)"CA-del(265-301)" [substitution]
    *** source: 9677429-Fig-2e
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xMek1(mnr)"CA"
    *** cells: HEK293 in BMS
    *** inhibited by: xMek1(mnr)"CA-del(265-301)" [substitution]
    *** source: 9677429-Fig-3a

contradictions

  *** xErk1[tAb]IP IVKA(MBP)[32P-ATP] is unchanged itpo xMek1
    *** cells: HEK293 in BMS
    *** unaffected by: xMek1(K97M) [substitution]
    *** source: 10232608-Fig-4c

chemicals

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xMek1(mnr)"CA"
    *** cells: H197 in BMS
    *** unaffected by: Rottlerin [pretreatment]
    *** source: 10330161-Fig-9d

no extras

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xMek1(del(32-51)/S218E/S222D)
    *** cells: COS1 in BMS
    *** source: 8622669-Fig-2b
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xMek1(S218E/S222E)"CA"
    *** cells: NIH3T3 in BMS
    *** source: 10669751-Fig-5a
  *** xErk2[Ab]IP IVKA(MBP)[32P-ATP] is increased itpo xMek1(S218E/S222E)"CA"
    *** cells: COS7 in BMS
    *** source: 8805275-Fig-5a
  *** xErk2[Ab]IP IVKA(MBP)[32P-ATP] is increased itpo xMek1(S218D/S222D)"CA"
    *** cells: REF52 in BMS
    *** source: 10480934-Fig-3d
  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is increased itpo xMek1
    *** cells: HEK293 in BMS
    *** source: 10187783-Fig-7a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased itpo xMek1(S218D/S222D)"CA"
    *** cells: HEK293E in BMLS
    *** source: 17360704-Fig-2b


==============
Erks phos(TEY)
==============

Brap--  ???

  *** Erks[phosAb] phos(TEY)[phosAb] is increased itpo xMek1(mnr)"CA"
    *** cells: HELA in BMS
    *** unaffected by: xBrap [addition]
    *** source: 14724641-Fig-2a

does not req Hras--

  *** xErk2[tAb]IP phos(TEY)[phosAb] is increased itpo xMek1(S218D/S222D)"CA"
    *** cells: COS1 in BMLS
    *** unaffected by: xHras(S17N)"DN" [addition]
    *** source: 12167697-Fig-2b,4a

does not req Pkce--

  *** xErk1[tAb]IP phos(TEY)[phosAb] is increased itpo xMek1(S218E/S222E/del(32-51))"CA"
    *** cells: HC11 in BMS
    *** unaffected by: xPkce(A159E)"CA" [addition]
    *** source: 11551901-Fig-4b

reqs Pkcz--

  *** xErk1[tAb]IP phos(TEY)[phosAb] is increased itpo xMek1(S218E/S222E/del(32-51))"CA"
    *** cells: HC11 in BMS
    *** inhibited by: xPkcz(K275W)"DN" [addition]
    *** source: 11551901-Fig-4b

Mek1 required features

  *** Erks[phosAb] phos(TEY)[phosAb] is increased itpo xMek1(mnr)"CA"
    *** cells: HEK293 in BMS
    *** inhibited by: xMek1(mnr)"CA-del(265-301)" [substitution]
    *** source: 9677429-Fig-2d

No extras

  *** Erks[phosAb] phos(TEY)[phosAb] is increased itpo xMek1
    *** cells: mEFs in BMS
    *** source: 16698790-Fig-6b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased itpo xMek1(mnr)"CA"
    *** cells: HEK293T<xEgfR> in BMLS
    *** source: 12446727-Fig-7

===============
Coprecipitation
===============

  *** xErk2[tAb] copptby[WB] xMek1[tAb] is detectable
    *** cells: COS1 in BMLS
    *** enhanced by: xRac1(Q61L)"CA" [addition]
    *** source: 12167697-Fig-1b,5b
  *** xMek1[tAb] copptby[WB] xErk2[tAb] is detectable
    *** cells: COS1 in BMLS
    *** enhanced by: xRac1(Q61L)"CA" [addition]
    *** enhanced by: xCdc42(Q61L)"CA" [addition]
    *** unaffected by: xRhoa(Q63L)"CA" [addition]
    *** unaffected by: xMek1(S218D/S222D)"KD" [substitution]
    *** source: 12167697-Fig-5a
  *** xMek1[tAb] copptby[WB] xErk1[tAb] is detectable
    *** cells: COS1<xMek1><xErk1> in BMLS "soluble-cytoplasmic fraction"
    *** enhanced by: xLamtor3 [addition]
    *** source: 9733512-Fig-6

==============
Basal Activity
==============

  *** xErk2[tAb]IP IVKA(MBP)[32P-ATP] is detectable
    *** cells: PC12 in BMLS
    *** inhibited by: xMek1(K97R)"DN" [addition]
    *** source: 9094716-Fig-2d


