  *** xTsc2[tAb] copptby[WB] xTsc1[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2> in BMS
    *** comment: IP is disrupted by addition of rErk2{act] and kinase buffer
    *** comment: substitution with xTsc2(S540A/S664A) prevents disruption by rErk2{act}
    *** source: 15851026-Fig-4d

  *** xTsc1[tAb] copptby[WB] xTsc2[tAb] is decreased itpo xAkt1(mnr)"CA"
    *** cells: DROSOPHILAS2<xTsc1><xTsc2> in BMLS
    *** reversed by: xTsc2(T437A/S924A/T1054A/T1518A)"dAktP" [substitution]
    *** comment: aa numbers refer to drosophila Tsc2 Q9U9B0
    *** comment: drome S924 corresponds to human S981
    *** comment: drome T1518 corresponds to human T1492
    *** source: 12172554-Fig-2c

============================================
Tsc1 phos(S487) phos(S511) disrupts complex
============================================

  *** xTsc1[tAb] copptby[WB] Tsc2[Ab] is detectable
    *** cells: MCF7<xTsc1> in BMS
    *** unaffected by: xTsc1(S487A/S511A) [substitution]
    *** partially inhibited by: xTsc1(S487D/S511D) [substitution]
    *** source: 17693255-Fig-S10c

============================================
Tsc2 phos(S540) phos(S664) disrupts complex
============================================

  *** xTsc2[tAb] copptby[WB] xTsc1[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2> in BMS
    *** enhanced by: xTsc2(S540A/S664A) [substitution]
    *** inhibited by: xTsc2(S540D/S664D) [substitution]
    *** source: 15851026-Fig-4a
  *** xTsc2[tAb] copptby[WB] xTsc1[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2> in BMS
    *** enhanced by: xTsc2(S540A/S664A) [substitution]
    *** source: 15851026-Fig-4b
  *** Tsc1[Ab] copptby[WB] xTsc2(S939A/S981A/S993A/S1130A/S1132A/S1462A/S1798A)[tAb] is detectable
    *** cells: HEK293T<xTsc2(S939A/S981A/S993A/S1130A/S1132A/S1462A/S1798A)> in BMS
    *** unaffected by: xTsc2(S939D/S981D/S993D/S1130D/S1132D/S1462D/S1798D) [substitution]
    *** source: 12167664-Fig-2d
  *** Tsc1[Ab] copptby[WB] xTsc2[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xTsc2(400-1745) [substitution]
    *** inhibited by: xTsc2(600-1745) [substitution]
    *** source: 15340059-Fig-4c
  *** xTsc2[tAb] copptby[WB] xTsc1[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xTsc2(R261P) [substitution]
    *** inhibited by: xTsc2(R611Q) [substitution]
    *** inhibited by: xTsc2(E814K) [substitution]
    *** inhibited by: xTsc2(R905Q) [substitution]
    *** inhibited by: xTsc2(R1200W) [substitution]
    *** inhibited by: xTsc2(N1645K) [substitution]
    *** inhibited by: xTsc2(N1653S) [substitution]
    *** inhibited by: xTsc2(N1683K) [substitution]
    *** inhibited by: xTsc2(R1745P) [substitution]
    *** inhibited by: xTsc2(R1747Q) [substitution]
    *** inhibited by: xTsc2(R1751Q/R1753Q) [substitution]
    *** source: 12172553-Fig-2c
  *** xTsc2[tAb] copptby[WB] xTsc1[tAb] is detectable
    *** cells: HEK293 in BMS
    *** inhibited by: xTsc2(S939D/S1086D/S10688E/T1422E) [substitution]
    *** source: 12172553-Fig-5c

Tsc2 phos(S1379/S1383/S1387) does not disrupt complex

  *** xTsc2[tAb] copptby[WB] xTsc1[tAb] is detectable
    *** cells: HEK293<xTsc1><xTsc2> in BMS
    *** unaffected by: xTsc2(S1379A/S1383A/S1387A) [substitution]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 14651849-Fig-6b

