=======================
Phosphorylation on T412
=======================

inhibited by xPras40-- but reqs Pras40 [RNAi]  ????

  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E<xS6k1> in BMLS
    *** inhibited by: xPras40 [addition]
    *** inhibited by: xPras40(T246A) [addition]
    *** comment: inhibition by xPras40(T246A) is greater than xPras40(WT)
    *** source: 17386266-Fig-7c
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E<xS6k1> in BMLS
    *** inhibited by: xPras40 [addition]
    *** source: 17386266-Fig-5a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (10 min)
    *** cells: 3T3L1 in BMLS
    *** partially reqs: Pras40 [RNAi]
    *** source: 17277771-Fig-3d
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (10 min)
    *** cells: 3T3L1<xPras40> in BMLS
    *** partially inhibited by: xPras40(T246A) [substitution]
    *** source: 17277771-Fig-3d
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (20 min)
    *** cells: 3T3L1 in BMLS
    *** partially reqs: Pras40 [RNAi]
    *** source: 17277771-Fig-3a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (20 min)
    *** cells: HEPG2 in BMS
    *** partially reqs: Pras40 [RNAi]
    *** source: 17277771-Fig-3a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (times)
    *** cells: 3T3L1 in BMLS
    *** times: 0 10++ 120+ min
    *** comment: partially reqs Pras40 [RNAi] at 10 min only
    *** source: 17277771-Fig-3b

not Ddit4--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (2 hr)
    *** cells: U2OS in BMLS
    *** inhibited by: xDdit4 [addition]
    *** unaffected by: xDdit4(mnr)"RPAA" [addition]
    *** source: 18198340-Fig-4c

not Dkk1--


Mlst8--  ???

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMS
    *** partially reqs: Mlst8 [RNAi] no-bkg-control
    *** source: 17043309-Fig-S3

Pdpk1--  ???

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMS
    *** partially reqs: Pdpk1 [RNAi] no-bkg-control
    *** source: 17043309-Fig-S3

Pik3ca--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA in BMLS "Serum deprived"
    *** partially reqs: Pik3ca [RNAi]
    *** source: 16176982-Fig-2e

not Sin1--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293 in BMS
    *** does not req: Sin1 [RNAi]
    *** source: 17043309-Fig-2b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMS
    *** does not req: Sin1 [RNAi]
    *** source: 17043309-Fig-2a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMS
    *** does not req: Sin1 [RNAi] no-bkg-control
    *** source: 17043309-Fig-S3
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: mEFs in BMLS
    *** does not req: Sin1 [KO]
    *** source: 16962653-Fig-5a

Rab5a--  ???

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293E in BMLS
    *** partially inhibited by: xRab5a(Q79L)"CA" [addition]
    *** source: 20053679-Fig-1g
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293E<xS6k1> in BMLS
    *** partially inhibited by: xRab5a [addition]
    *** partially inhibited by: xRab5a(S34N)"DN" [addition]
    *** inhibited by: xRab5a(Q79L)"CA" [addition]
    *** source: 20053679-Fig-1a
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293E<xS6k1> in BMLS
    *** inhibited by: xRab5a(Q79L)"CA" [addition]
    *** source: 20053679-Fig-2a

Rheb--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA in BMLS "Serum deprived"
    *** reqs: Rheb [RNAi]
    *** source: 16176982-Fig-2a
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA<xS6k1> in BMLS
    *** enhanced by: xRheb [addition]
    *** source: 12820960-Fig-2c

not Rictor--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293 in BMS
    *** does not req: Rictor [RNAi]
    *** source: 17043309-Fig-2b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMS
    *** does not req: Rictor [RNAi]
    *** source: 17043309-Fig-2a

Rrags-- ???

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (10 min)
    *** cells: HELA in BMS
    *** partially reqs: RagC and/or RagD [RNAi]
    *** source: 18497260-Fig-S6b
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Ins (10 min)
    *** cells: HEK293E<xS6k1><xRagB(Q99L)"GTP"><xRagC(S75L)"GDP"> in BMLS
    *** inhibited by: xRagB(T54L)"GDP" + xRagC(Q120L)"GTP" [substitution]
    *** source: 18497260-Fig-2c

Tbk1--  ???

  *** S6ks[phosAb] phos(FTYV)[phosAb] is increased irt Ins (30 min)
    *** cells: mEFs<Rictor~null><xTbk1> in BMLS
    *** partially reqs: xTbk1 [omission]
    *** source: 21464307-Fig-5a

not Tsc1-- and/or Tsc2--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA in BMLS "Serum deprived"
    *** does not req: Tsc2 [RNAi]
    *** source: 16176982-Fig-1c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: mEFs in BMLS "Serum deprived"
    *** does not req: Tsc2 [KO]
    *** source: 16176982-Fig-1d
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293T<xS6k1> in BMLS
    *** bkg inhibited by: xTsc1 + xTsc2 [addition]
    *** bkg inhibited by: xTsc1 + xTsc2(S1254A) [addition]
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 12468542-Fig-4b
  *** S6k1[phosAb] phos(T412)[phosAb] is detectable-but unchanged irt Ins (30 min)
    *** cells: mEFs<Tsc2~null> in BMLS
    *** source: 15772076-Fig-3f

Vps34--

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA in BMLS "Serum deprived"
    *** reqs: Vps34 [RNAi]
    *** source: 16176982-Fig-4c

chemicals

  *** S6k1[phosAb] phos(T412)[phosAb] is detectable
    *** cells: HC11 in BMLS
    *** inhibited by: LY294002 [addition]
    *** source: 16984645-Fig-5
  *** S6k1[phosAb] phos(T412)[phosAb] is detectable
    *** cells: HC11 in BMLS
    *** inhibited by: Rapamycin [addition]
    *** source: 16984645-Fig-5
  *** S6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Ins (times)
    *** cells: HC11 in BMLS
    *** times: 0 7+ 15+ min
    *** partially inhibited by: LY294002 [addition]
    *** partially inhibited by: Rapamycin [addition]
    *** source: 16984645-Fig-5
  *** S6ks[phosAb] phos(FTYV)[phosAb] is increased irt Ins (30 min)
    *** cells: mEFs<Rictor~null><xTbk1> in BMLS
    *** inhibited by: LY294002 [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 21464307-Fig-5a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (90 min)
    *** cells: mEFs in BMLS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 18198340-Fig-2b
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA<xS6k1> in BMLS
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** bkg inhibited by: Wortmannin [pretreatment]
    *** source: 12820960-Fig-4a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: NIH3T3<xInsR> in BMLS
    *** bkg inhibited by: Wortmannin [addition]
    *** bkg inhibited by: Rapamycin [addition]
    *** source: 12820960-Fig-2b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: KB in BMLS
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** unaffected by: U0126 [pretreatment]
    *** source: 15024086-Fig-2b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: KB in BMLS
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** source: 15024086-Fig-6b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (4 hr)
    *** cells: ARPE19 in BMLS
    *** inhibited by: Aicar [cotreatment]
    *** source: 16516166-Fig-3
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA in BMLS
    *** inhibited by: Rapamycin [pretreatment]
    *** unaffected by: U0126 [pretreatment]
    *** inhibited by: Rapamycin + U0126 [pretreatment]
    *** source: 16763566-Fig-1a



S6k1 features

  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(1-421)"delCT" [substitution]
    *** inhibited by: xS6k1(47-525)"delNT" [substitution]
    *** bkg inhibited by: xS6k1(47-421) [substitution]
    *** source: 9632736-Fig-4a
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(T412A) [substitution]
    *** bkg inhibited by: xS6k1(T412E) [substitution]
    *** source: 9632736-Fig-5a
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** unaffected by: xS6k1(K123M) [substitution]
    *** bkg inhibited by: xS6k1(T256A) [substitution]
    *** bkg inhibited by: xS6k1(S394A) [substitution]
    *** bkg inhibited by: xS6k1(S394D) [substitution]
    *** source: 9632736-Fig-5a

other stim

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (10 min)
    *** cells: MC3T3E1 in BMS
    *** unaffected by: Dkk1 [pretreatment]
    *** source: 16959574-Fig-S4a

reqs Glucose

  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA<xS6k1> in BMLS
    *** bkg inhibited by: 2DOG [pretreatment]
    *** source: 12820960-Fig-4a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: mEFs in BMLS
    *** inhibited by: 2DOG [posttreatment]
    *** inhibited by: 5ThioGlucose [posttreatment]
    *** source: 16027121-Fig-6a


reqs AminoAcids except in 16541103 using adipocytes

  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Ins (10 min)
    *** cells: HEK293E<xS6k1> in BMLS
    *** inhibited by: AA-deprivation [pretreatment]
    *** source: 18497260-Fig-2d
  *** S6k1[phosAb] phos(T412)[phosAb] is unchanged irt Ins (30 min)
    *** cells: HEK293 in BMLS "Serum and AminoAcid deprived"
    *** source: 16176982-Fig-1b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (5 hr)
    *** cells: HEK293T in BMLS
    *** inhibited by: Hypoxia [pretreatment]
    *** source: 18198340-Fig-2d
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA<xS6k1> in BMLS
    *** bkg inhibited by: AA-deprivation [pretreatment]
    *** source: 12820960-Fig-4a
  *** S6k1[Ab] phos[WBMS] is increased irt Ins (30 min)
    *** cells: CHOK1 in BMLS
    *** comment: unaffected by replacing BMLS with BSS+Glucose + AminoAcids for 30 min before Ins
    *** comment: bkg inhibited by replacing BMLS with BSS+Glucose - AminoAcids for 30 min before Ins
    *** comment: unaffected by replacing BMLS with BSS+Glucose + CHX for 30 min before Ins
    *** source: 15024086-Fig-4a
  *** S6k1[Ab] phos[WBMS] is increased irt Ins (30 min)
    *** cells: KB in BMLS
    *** comment: bkg inhibited by replacing BMLS with BSS+Glucose - AminoAcids for 4 h4 before Ins
    *** comment: unaffected by replacing BMLS with BSS+Glucose + AminoAcids for 4 hr before Ins
    *** source: 15024086-Fig-4b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: mEFs in BMLS
    *** inhibited by: AA-deprivation (90 min) [pretreatment]
    *** source: 15772076-Fig-3f

no extras

  *** S6k1[Ab] phos[WBMS] is increased irt Ins (tnr)
    *** cells: CHOK1 in BMS
    *** source: 12194824(D)
  *** xS6k1[phosAb] phos(T252)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293 in BMLS
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 11914378-Fig-1b
  *** xS6k1[phosAb] phos(T252)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293 in BMLS
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 11914378-Fig-3
  *** xS6k1[phosAb] phos(S394)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293 in BMLS
    *** comment: aa numbers converted to Uniprot human canonical sequence
    *** source: 11914378-Fig-2a
  ----------------- phos(T412) -----------------------------------------
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293 in BMS
    *** source: 17043309-Fig-2b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMS
    *** source: 17043309-Fig-2a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293 in BMS
    *** source: 17043309-Fig-2b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMS
    *** source: 17043309-Fig-2a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (45 min)
    *** cells: HEK293 in BMLS
    *** source: 12777372-Fig-1c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA in BMLS
    *** source: 16049009-Fig-5a
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293 in BMS
    *** source: 12172553-Fig-7a
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293 in BMS
    *** source: 12172553-Fig-7b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (5 min)
    *** cells: 3T3L1 in BMLS "AminoAcid deprived" "differentiated into adipocytes"
    *** source: 16541103-Fig-8c
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293 in BMS
    *** source: 12172553-Fig-1b
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293 in BMS
    *** source: 12172553-Fig-2a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293 in BMS
    *** source: 12172553-Fig-2b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (times)
    *** cells: 3T3L1 in BMLS "AminoAcid deprived" "differentiated into adipocytes"
    *** times: 0 1 3+ 5++ 15++ 30++ 60++ min
    *** source: 16541103-Fig-7a
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293E in BMLS
    *** source: 16286006-Fig-2b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (times)
    *** cells: HEK293E in BMLS
    *** times: 0 2 5+ 10+ 20+ 30+ min
    *** source: 16286006-Fig-5a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (times)
    *** cells: HC11 in BMLS
    *** times: 0 7+ 15+ min
    *** source: 16984645-Fig-5
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** source: 12150925-Fig-S3
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** source: 17386266-Fig-1a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E in BMLS
    *** source: 17386266-Fig-1c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HEK293E<xRaptor> in BMLS
    *** source: 17386266-Fig-1d
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: HELA in BMLS
    *** source: 17386266-Fig-1c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: mEFs in BMLS
    *** source: 17386266-Fig-1c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: mEFs in BMLS
    *** source: 17386266-Fig-1e
  *** xS6k1[tAb]IP phos(T412)[phosAb] is increased irt Ins (10 min)
    *** cells: HEK293E<xS6k1><xRap2a> in BMLS
    *** source: 18497260-Fig-2c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** source: 17510057-Fig-3b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** source: 17510057-Fig-3c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (times)
    *** cells: 3T3L1 in BMLS "differentiated into adipocytes"
    *** times: 0 5+ 10++ 30++ min
    *** source: 17510057-Fig-3a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: mEFs in BMS
    *** source: 16959574-Fig-S5d
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: RIE in BMS
    *** source: 16959574-Fig-S5d
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (times)
    *** cells: mEFs in BMLS
    *** times: 0 5+ 15++ 30++ 60++ 120++ min
    *** source: 16959574-Fig-1b
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA in BMS
    *** source: 18604198-Fig-S3
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA in BMS "AminoAcid-deprived"
    *** source: 18604198-Fig-S3
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: HELA<xS6k1> in BMLS
    *** source: 18604198-Fig-3c
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (times)
    *** cells: CHO<xInsR> in BMLS
    *** times: 0 1 2 5+ 10++ 20+ 30+ min
    *** source: 9632736-Fig-3a
  *** xS6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (times)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** times: 0 2+ 5+ 10++ 15++ 20++ 30++ min
    *** source: 9632736-Fig-2a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (times)
    *** cells: HELA in BMLS
    *** times: 0 3 5 10+ 15++ 30++ min
    *** source: 16763566-Fig-1b

===========================
Other Phosphorylation Sites
===========================

  ----------------- phos(T252) -----------------------------------------
  *** xS6k1[phosAb] phos(T252)[phosAb] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(1-421)"delCT" [substitution]
    *** bkg inhibited by: xS6k1(47-525)"delNT" [substitution]
    *** inhibited by: xS6k1(47-421) [substitution]
    *** source: 9632736-Fig-4a
  *** xS6k1[phosAb] phos(T252)[phosAb] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(K123M) [substitution]
    *** unaffected by: xS6k1(T256A) [substitution]
    *** bkg inhibited by: xS6k1(S394A) [substitution]
    *** bkg inhibited by: xS6k1(S394D) [substitution]
    *** source: 9632736-Fig-5a
  *** xS6k1[phosAb] phos(T252)[phosAb] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(T412A) [substitution]
    *** inhibited by: xS6k1(T412E) [substitution]
    *** source: 9632736-Fig-5a
  *** xS6k1[phosAb] phos(T252)[phosAb] is increased irt Ins (times)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** times: 0+ 2++ 5++ 10++ 15++ 20++ 30++ min
    *** source: 9632736-Fig-2a
  ----------------- phos(T434) -----------------------------------------
  *** xS6k1[phosAb] phos(T434)[phosAb] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(1-421)"delCT" [substitution]
    *** unaffected by: xS6k1(47-525)"delNT" [substitution]
    *** bkg inhibited by: xS6k1(47-421) [substitution]
    *** source: 9632736-Fig-4a
  *** xS6k1[phosAb] phos(T434)[phosAb] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** unaffected by: xS6k1(K123M) [substitution]
    *** unaffected by: xS6k1(T256A) [substitution]
    *** unaffected by: xS6k1(S394A) [substitution]
    *** unaffected by: xS6k1(S394D) [substitution]
    *** source: 9632736-Fig-5a
  *** S6k1[phosAb] phos(T434)[phosAb] is increased irt Ins (times)
    *** cells: CHO<xInsR> in BMLS
    *** times: 0+ 1+ 2+ 5+ 10++ 20++ 30++ min
    *** source: 9632736-Fig-3a
  *** xS6k1[phosAb] phos(T434)[phosAb] is increased irt Ins (times)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** times: 0+ 2++ 5++ 10++ 15++ 20+++ 30+++ min
    *** source: 9632736-Fig-2a
  ----------------- phos(T444/S447) -----------------------------------------
  *** S6k1[phosAb] phos(T444/S447)[phosAb] is unchanged irt Ins (45 min)
    *** cells: HEK293 in BMLS
    *** source: 12777372-Fig-1c
  *** xS6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293 in BMLS
    *** source: 11914378-Fig-2a
  *** S6k1[phosAb] phos(T444/S447)[phosAb] is unchanged irt Ins (30 min)
    *** cells: HELA in BMLS
    *** source: 16049009-Fig-5a
  *** xS6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Ins (30 min)
    *** cells: HEK293 in BMS
    *** source: 12172553-Fig-1b
  *** xS6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(1-421)"delCT" [substitution]
    *** unaffected by: xS6k1(47-525)"delNT" [substitution]
    *** bkg inhibited by: xS6k1(47-421) [substitution]
    *** source: 9632736-Fig-4a
  *** xS6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(K123M) [substitution]
    *** unaffected by: xS6k1(T256A) [substitution]
    *** bkg inhibited by: xS6k1(S394A) [substitution]
    *** bkg inhibited by: xS6k1(S394D) [substitution]
    *** source: 9632736-Fig-5a
  *** S6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Ins (times)
    *** cells: CHO<xInsR> in BMLS
    *** times: 0 1 2 5+ 10+ 20+ 30+ min
    *** source: 9632736-Fig-3a
  *** xS6k1[phosAb] phos(T444/S447)[phosAb] is increased irt Ins (times)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** times: 0 2+ 5+ 10++ 15++ 20++ 30++ min
    *** source: 9632736-Fig-2a


===============
Kinase Activity
===============

  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (15 min)
    *** cells: HEK293 in BMS
    *** source: 15342917-Fig-5c
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (15 min)
    *** cells: HEK293<xTsc2> in BMS
    *** partially inhibited by: xTsc2(S1798A) [substitution]
    *** partially inhibited by: xTsc2(S939A/T1462A) [substitution]
    *** partially  inhibited by: xTsc2(S939A/T1462A/S1798A) [substitution]
    *** source: 15342917-Fig-5c
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (15 min)
    *** cells: HEK293<xS6k1> in BMLS
    *** bkg inhibited by: Wortmannin [pretreatment]
    *** source: 15342917-Fig-1a
  *** xS6k1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Ins (30 min)
    *** cells: GRCLR73 in BMLS
    *** source: 16049009-Fig-1d
  *** xS6k1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293T in BMLS
    *** source: 16049009-Fig-1a
  *** xS6k1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEPG2 in BMLS
    *** source: 16049009-Fig-1h
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293 in BMLS
    *** source: 11914378-Fig-1b
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293 in BMLS
    *** source: 11914378-Fig-3
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (20 min)
    *** cells: CHO<xInsR> in BMLS
    *** source: 9427642-Fig-1b
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293 in BMS
    *** source: 12172553-Fig-1a
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293 in BMS
    *** source: 12172553-Fig-7c
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293E in BMLS
    *** source: 16286006-Fig-2b
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293E<xS6k1> in BMLS
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 12906785-Fig-3
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293E<xS6k1> in BMLS
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 12906785-Fig-4
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293E<xS6k1> in BMLS
    *** inhibited by: Rapamycin [pretreatment]
    *** source: 12906785-Fig-1a
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293E<xS6k1> in BMLS
    *** partially inhibited by: xS6k1(mnr)"F5adCT" [substitution]
    *** source: 12906785-Fig-4
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293E<xS6k1> in BMLS
    *** source: 12906785-Fig-5a
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293E<xS6k1> in BSS "containing Glucose"
    *** inhibited by: Rapamycin [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 12906785-Fig-3
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(K123M) [substitution]
    *** bkg inhibited by: xS6k1(T256A) [substitution]
    *** bkg inhibited by: xS6k1(S394A) [substitution]
    *** bkg inhibited by: xS6k1(S394D) [substitution]
    *** source: 9632736-Fig-5a
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** bkg inhibited by: xS6k1(T412A) [substitution]
    *** unaffected by: xS6k1(T412E) [substitution]
    *** source: 9632736-Fig-5a
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (10 min)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** unaffected by: xS6k1(1-421)"delCT" [substitution]
    *** bkg inhibited by: xS6k1(47-525)"delNT" [substitution]
    *** unaffected by: xS6k1(47-421) [substitution]
    *** source: 9632736-Fig-4a
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (times)
    *** cells: CHO<xInsR><xS6k1> in BMLS
    *** times: 0+ 2++ 5++ 10++ 15++ 20++ 30++ min
    *** source: 9632736-Fig-2a
  *** S6k1[Ab]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (times)
    *** cells: CHO<xInsR> in BMLS
    *** times: 0 1 2 5+ 10++ 20+ 30+ min
    *** source: 9632736-Fig-3a


====================
Other initial states
====================

  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (30 min)
    *** cells: mHepatocytes in BMLS "AminoAcid and Glucose deprived"
    *** bkg inhibited by: Rapamycin [pretreatment]
    *** source: 16763566-Fig-2a
  *** S6k1[phosAb] phos(T412)[phosAb] is increased irt Ins (15 min)
    *** cells: 3T3L1 in BMLS "AminoAcid deprived" "differentiated into adipocytes"
    *** inhibited by: Rapamycin [addition]
    *** unaffected by: FK506 [addition]
    *** unaffected by: Rapamycin + FK506 [addition]
    *** source: 16541103-Fig-3e
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Ins (30 min)
    *** cells: HEK293E<xS6k1> in BSS "containing Glucose and AminoAcids"
    *** inhibited by: Rapamycin [pretreatment]
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 12906785-Fig-3
