==========================================
TgfbR1 can be bound by Tgfb1, Tgfb2, Tgfb3
==========================================

  *** TgfbR1[MW] boundby[Xlink] Tgfb1[125I] is detectable
    *** cells: DR27<TgfbR2~null><xTgfbR2> in BMLS
    *** inhibited by: Tgfb1 [addition]
    *** inhibited by: Tgfb2 [addition]
    *** inhibited by: Tgfb3 [addition]
    *** comment: Tgfb2 has a lower affinity for TgfbR1 than Tgfb1 or Tgfb3
    *** source: 1333888-Fig-6a
  *** TgfbR1[MW] boundby[Xlink] Tgfb1[125I] is detectable
    *** cells: MV1LU in BMLS
    *** inhibited by: Tgfb1 [addition]
    *** inhibited by: Tgfb2 [addition]
    *** inhibited by: Tgfb3 [addition]
    *** comment: Tgfb2 has a lower affinity for TgfbR1 than Tgfb1 or Tgfb3
    *** source: 1333888-Fig-6a
  *** TgfbR1[MW] boundby[Xlink] Tgfb2[125I] is detectable
    *** cells: RAT1 in BMLS
    *** source: 7693660-Fig-1b

=============================================
TgfbR2 can be bound by Tgfb1, Tgfb3 not Tgfb2
=============================================

  *** xTgfbR2[MW] boundby[Xlink] Tgfb1[125I] is detectable
    *** cells: DR27<TgfbR2~null><xTgfbR2> in BMLS
    *** inhibited by: Tgfb1 [addition]
    *** inhibited by: Tgfb3 [addition]
    *** unaffected by: Tgfb2 [addition]
    *** source: 1333888-Fig-6a
  *** TgfbR2[MW] boundby[Xlink] Tgfb1[125I] is detectable
    *** cells: MV1LU in BMLS
    *** inhibited by: Tgfb1 [addition]
    *** inhibited by: Tgfb3 [addition]
    *** unaffected by: Tgfb2 [addition]
    *** source: 1333888-Fig-6a
  *** TgfbR1[MW] boundby[Xlink] Tgfb1[125I] is detectable
    *** cells: RAT1 in BMLS
    *** source: 7693660-Fig-1a
  *** TgfbR2[MW] boundby[Xlink] Tgfb1[125I] is detectable
    *** cells: RAT1 in BMLS
    *** source: 7693660-Fig-1a

==============================================
Binding of TgfbR1 and TgfbR2 irt Tgfb1
==============================================

  *** xTgfbR2[125I-Tgfb1-XL] copptby[WB] xTgfbR1[tAb] is detectable
    *** cells: COS1<xSmad7> in BMS
    *** does not req: xSmad7 [omission]
    *** unaffected by: xTgfbR1(K232R)"KD" [substitution]
    *** unaffected by: xTgfbR2(K277R)"KD" [substitution]
    *** source: 9335507-Fig-3
  *** TgfbR1[Ab] copptby[WB] TgfbR2[Ab] is increased irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 5+ 15+ 30+ 60+ min
    *** source: 14612425-Fig-2d
  *** TgfbR2[Ab] copptby[WB] TgfbR1[Ab] is increased irt Tgfb1 (times)
    *** cells: AKR2B in BMLS
    *** times: 0 5++ 15+ 30+ 60++ min
    *** source: 14612425-Fig-2d

=======================================================
TgfbR1 is phosphorylated by TgfbR2 in response to Tgfb1
=======================================================

requirement for TgfbR2 kinase activity

  *** xTgfbR1[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null><xTgfbR1><xTgfbR2> in BMLS "containing Tgfb1 (10 min)"
    *** inhibited by: xTgfbR2(K277R)"KD" [substitution]
    *** comment: xTgfbR1 is phosed on Serine and Threonine [PAA]
    *** source: 7774578-Fig-3a
  *** xTgfbR1(T204D)[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null><xTgfbR2> in BMLS "containing Tgfb1 (10 min)"
    *** inhibited by: xTgfbR2(K277R)"KD" [substitution]
    *** source: 7774578-Fig-6b
  *** xTgfbR1[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null><xTgfbR1><xTgfbR2> in BMLS "containing Tgfb1 (10 min)"
    *** inhibited by: xTgfbR2(K277R)"KD" [substitution]
    *** source: 7774578-Fig-4c

major TgfbR1 phos-sites are T200/T204

  *** xTgfbR1[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null><xTgfbR1><xTgfbR2> in BMLS "containing Tgfb1 (10 min)"
    *** inhibited by: xTgfbR1(T200V) [substitution]
    *** partially inhibited by: xTgfbR1(T204V) [substitution]
    *** source: 7774578-Fig-4c
  *** xTgfbR1[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null><xTgfbR1><xTgfbR2> in BMLS "containing Tgfb1 (10 min)"
    *** inhibited by: xTgfbR1(T185V/T186V/S187A/S189A/S191A) [substitution]
    *** partially inhibited by: xTgfbR1(T185V/T186V) [substitution]
    *** partially inhibited by: xTgfbR1(T185V/T186V/S187A) [substitution]
    *** partially inhibited by: xTgfbR1(S187A/S189A/S191A) [substitution]
    *** partially inhibited by: xTgfbR1(S189A/S191A) [substitution]
    *** source: 7774578-Fig-3a

TgfbR1 is not autophosphoryated on T204

  *** xTgfbR1[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xTgfbR1(K232R) [substitution]
    *** unaffected by: xTgfbR1(T204D) [substitution]
    *** source: 7774578-Fig-6c

=============================================
TgfbR2 is phosphorylated in response to Tgfb1
=============================================

does not require TgfbR2 kinase activity

  *** xTgfbR2[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null><xTgfbR1> in BMLS "containing Tgfb1 (10 min)"
    *** unaffected by: xTgfbR2(K277R)"KD" [substitution]
    *** source: 7774578-Fig-4c
  *** xTgfbR2[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null><xTgfbR1(T204D)> in BMLS "containing Tgfb1 (10 min)"
    *** unaffected by: xTgfbR2(K277R)"KD" [substitution]
    *** source: 7774578-Fig-6b
  *** xTgfbR2[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null><xTgfbR1><xTgfbR2> in BMLS "containing Tgfb1 (10 min)"
    *** partially inhibited by: xTgfbR2(K277R)"KD" [substitution]
    *** source: 7774578-Fig-3a

does not require TgfbR1 phos sites

  *** xTgfbR2[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null><xTgfbR1> in BMLS "containing Tgfb1 (10 min)"
    *** unaffected by: xTgfbR1(T200V) [substitution]
    *** unaffected by: xTgfbR1(T204V) [substitution]
    *** source: 7774578-Fig-4c
  *** xTgfbR2[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null><xTgfbR1><xTgfbR2> in BMLS "containing Tgfb1 (10 min)"
    *** partially inhibited by: xTgfbR1(T185V/T186V/S187A/S189A/S191A) [substitution]
    *** partially inhibited by: xTgfbR1(T185V/T186V) [substitution]
    *** partially inhibited by: xTgfbR1(T185V/T186V/S187A) [substitution]
    *** partially inhibited by: xTgfbR1(S187A/S189A/S191A) [substitution]
    *** partially inhibited by: xTgfbR1(S189A/S191A) [substitution]
    *** source: 7774578-Fig-3a


==================================================================
TgfbR1 associated kinase activity is increased in reponse to Tgfb1
==================================================================

  *** TgfbR1[Ab]IP IVKA(Smad2)[32P-ATP] is increased irt Tgfb1 (5 min)
    *** cells: AKR2B in BMLS
    *** source: 19289088-Fig-1c

==================================================================
TgfbR2 associated kinase activity is increased in reponse to Tgfb1
==================================================================

  *** TgfbR2[Ab]IP IVKA(Smad2)[32P-ATP] is increased irt Tgfb1 (30 min)
    *** cells: MV1LU in BMS
    *** unaffected by: rSmad2(S464A) [substitution]
    *** unaffected by: rSmad2(S465A) [substitution]
    *** unaffected by: rSmad2(S467A) [substitution]
    *** partially inhibited by: rSmad2(S464A/S465A) [substitution]
    *** partially inhibited by: rSmad2(S464A/S467A) [substitution]
    *** partially inhibited by: rSmad2(S465A/S467A) [substitution]
    *** source: 9346908-Fig-3a

===========================================================
Activation of the TgfbR1/TgfbR2 complex irt Tgfb1 requires:
TgfbR1 kinase activity
TgfbR1 phos-sites
===========================================================

  *** TRE-reporter[Luc] is increased irt Tgfb1 (20 hr)
    *** cells: DR26<TgfbR2~null><xTgfbR2> in BMLS
    *** source: 7774578-Fig-5c
  *** TRE-reporter[Luc] is increased irt Tgfb1 (20 hr)
    *** cells: R1B<TgfbR1~null><xTgfbR1><xTgfbR2> in BMLS
    *** inhibited by: xTgfbR1(K232R) [substitution]
    *** inhibited by: xTgfbR1(T185D/T186D/S187D/S189D/S191D) [substitution]
    *** inhibited by: xTgfbR1(T200D) [substitution]
    *** unaffected by: xTgfbR1(T204D) [substitution]
    *** source: 7774578-Fig-5a
  *** TRE-reporter[Luc] is increased irt Tgfb1 (20 hr)
    *** cells: R1B<TgfbR1~null><xTgfbR1><xTgfbR2> in BMLS
    *** inhibited by: xTgfbR1(K232R) [substitution]
    *** inhibited by: xTgfbR1(T185V/T186V/S187A/S189A/S191A/T200V/T204V) [substitution]
    *** inhibited by: xTgfbR1(T185V/T186V/S187A/S189A/S191A) [substitution]
    *** inhibited by: xTgfbR1(T186V/S187A/S189A/S191A) [substitution]
    *** partially inhibited by: xTgfbR1(T185V/T186V/S187A) [substitution]
    *** partially inhibited by: xTgfbR1(S187A/S189A/S191A) [substitution]
    *** partially inhibited by: xTgfbR1(T185V/T186V) [substitution]
    *** partially inhibited by: xTgfbR1(S189A/S191A) [substitution]
    *** unaffected by: xTgfbR1(T185V) [substitution]
    *** unaffected by: xTgfbR1(T186V) [substitution]
    *** unaffected by: xTgfbR1(S187A) [substitution]
    *** unaffected by: xTgfbR1(S189A) [substitution]
    *** unaffected by: xTgfbR1(S191A) [substitution]
    *** source: 7774578-Fig-2a
  *** TRE-reporter[Luc] is increased irt Tgfb1 (20 hr)
    *** cells: R1B<TgfbR1~null><xTgfbR1><xTgfbR2> in BMLS
    *** inhibited by: xTgfbR1(K232R) [substitution]
    *** inhibited by: xTgfbR1(T200V) [substitution]
    *** partially inhibited by: xTgfbR1(T204V) [substitution]
    *** source: 7774578-Fig-4a

======================================
TgfbR1 T204 is an activating phos-site
======================================

  *** TRE-reporter[Luc] is increased itpo xTgfbR1(T204D)
    *** cells: DR26<TgfbR2~null> in BMLS
    *** inhibited by: xTgfbR1(WT) [substitution]
    *** source: 7774578-Fig-5c
  *** xTgfbR1[tAb]IP phos[32Pi] is detectable
    *** cells: R1B<TgfbR1~null> in BMLS
    *** enhanced by: xTgfbR1(T204D) [substitution]
    *** source: 7774578-Fig-6a










