===============
Kinase Activity
===============

Gab1-- or Gab2--

  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: MCF7 in BMLS
    *** reqs: Gab1 [RNAi]
    *** reqs: Gab2 [RNAi]
    *** reqs: Gab1 and/or Gab2 [DRNAi]
    *** source: 15952937-Fig-4d
  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: T47D in BMLS
    *** partially reqs: Gab1 [RNAi]
    *** partially reqs: Gab2 [RNAi]
    *** reqs: Gab1 and/or Gab2 [DRNAi]
    *** source: 15952937-Fig-4a
  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: T47D in BMLS
    *** reqs: Gab1 [RNAi]
    *** source: 15952937-Fig-4b
  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: T47D in BMLS
    *** reqs: Gab1 [RNAi]
    *** source: 15952937-Fig-4c
  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: T47D in BMLS
    *** reqs: Gab1 [RNAi]
    *** comment: req for Gab1 can be overcome with xGab2
    *** source: 15952937-Fig-5a
  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: T47D in BMLS
    *** reqs: Gab2 [RNAi]
    *** comment: req for Gab2 can be overcome with xGab1
    *** source: 15952937-Fig-5b
  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: T47D in BMLS
    *** reqs: Gab1 [RNAi]
    *** comment: req for Gab1 can not be overcome with xIrs1
    *** source: 15952937-Fig-6
  *** Mek1[Ab]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: T47D in BMLS
    *** partially reqs: Gab1 [RNAi]
    *** partially reqs: Gab2 [RNAi]
    *** reqs: Gab1 and/or Gab2 [DRNAi]
    *** source: 15952937-Fig-7b

Rac2--  ???

  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 5+++ 10++ 15+ 30 60 120 min
    *** enhanced by: xRac2(G12V)"CA" [addition]
    *** source: 9351825-Fig-8a

Mek1 features--

  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (times)
    *** cells: HEK293 in BMS
    *** times: 0 2++ 5++ 10+ min
    *** unaffected by: xMek1(mnr)"CA-del(265-301)" [substitution]
    *** source: 9677429-Fig-5d

no extras

  *** Mek1[Ab]IP IVKA(Erks)[32P-ATP] is increased irt Egf (tnr)
    *** cells: REF52 in BMS
    *** source: 10480934-Fig-5d
  *** Mek1[Ab]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293 in BMS
    *** source: 7624324-Fig-5a
  *** xMek1[tAb]IP IVKA(Erk2,MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293 in BMS
    *** source: 7624324-Fig-5b
  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HEK293 in BMS
    *** source: 7624324-Fig-5b
  *** xMek1[tAb]IP IVKA(Erk?)[32P-ATP] is increased irt Egf (5 min)
    *** cells: mEFs<Ksr1~null><xKsr1><xMek1> in BMLS
    *** source: 19541618-Fig-S2

=============================
Phosphorylation at SMANS site
=============================

Braf--  ???

  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Ksr1~null><xKsr1><xBraf> in BMLS
    *** times: 0 5++ 20+ min
    *** unaffected by: xBraf(S151A) [substitution]
    *** unaffected by: xBraf(S750A/T753A) [substitution]
    *** comment: enhanced at 20 min by xBraf(S151A/T401A/S750A/T753A) [substitution]
    *** source: 19541618-Fig-S3b

not Brap--

  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMS
    *** times: 0 10+++ 20++ 30+ min
    *** does not req: Brap [RNAi]
    *** source: 14724641-Fig-2f
  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 10++ 30+ min
    *** does not req: Brap [KO]
    *** source: 14724641-Fig-2g

Gab1--

  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 5++ 15++ 30 min
    *** partially reqs: Gab1 [KO]
    *** source: 12629518-Fig-2a
  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1+ 3+++ 4+++ 5+++ 10++ 20+ 30+ min
    *** partially reqs: Gab1 [RNAi]
    *** source: 16687399-Fig-8c

Git1-- ???

  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 2+ 5++ 10+ min
    *** enhanced by: xGit1 [addition]
    *** source: 14701758-Fig-6b

IqGap1--  ???

  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (10 min)
    *** cells: MCF7 in BMLS
    *** unaffected by: xIqGap1 [addition]
    *** bkg reqs: IqGap1 [RNAi]
    *** source: 16135787-Fig-3a

not Ikk1--

  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 15++ 30++ 60+ min
    *** does not req: Ikk1 [KO]
    *** source: 15155743-Fig-3a

Ksr1-- ???

  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Ksr1~null><xKsr1><xBraf> in BMS
    *** times: 0 10++ 20+ min
    *** comment: enhanced at 20 min by xKsr1(T260A/T274A/S320A/S443A)"hT272A/T286A/S197A/S455A [substitution]
    *** source: 19541618-Fig-4d
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Egf (5 min)
    *** cells: A431 in BMLS
    *** inhibited by: xKsr1(D683A/D700A)"KD" [addition]
    *** source: 19766101-Fig-2d
  *** xMek1[phosAb] phos(SMANS)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs<Ksr1~null><xMek1> in BMLS
    *** enhanced by: xKsr1 [addition]
    *** source: 19541618-Fig-2d
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs<Ksr1~null> in BMLS
    *** enhanced by: xKsr1 [addition]
    *** source: 19541618-Fig-2c
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Egf (times)
    *** cells: mEFs<Ksr1~null><xKsr1> in BMLS
    *** times: 0 5+ 10+ 15+ 30 min
    *** does not req: xKsr1 [omission]
    *** comment: Mek12s phos is slightly less at 15 min itao xKsr1
    *** source: 18332145-Fig-4a

Mlk3--

  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (10 min)
    *** cells: CCD18CO in BMLS
    *** reqs: Mlk3 [RNAi]
    *** source: 15258589-Fig-2a
  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (10 min)
    *** cells: WI38 in BMLS
    *** reqs: Mlk3 [RNAi]
    *** source: 15258589-Fig-2c

not Pak1-- or Pak2--

  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (5 min)
    *** cells: HELA in BMS
    *** does not req: Pak1 or Pak2 [DRNAi]
    *** source: 16129686-Fig-3a
  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (5 min)
    *** cells: NIH3T3 in BMLS
    *** unaffected by: xPak1(83-149)"DN" [addition]
    *** source: 16129686-Fig-3a
  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 5+++ 10++ 15+ 30 60 120 min
    *** enhanced by: xPak1(165-544)"CA" [addition]
    *** source: 9351825-Fig-8a

not Raf1--

  *** Mek1[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (5 min)
    *** cells: rEFs in BMLS
    *** does not req: Raf1 [RNAi]
    *** source: 17314031-Fig-3
  *** Mek12s[Ab]IP IVKA(Erk?,MBP)[32P-ATP] is increased irt Egf (5 min)
    *** cells: mEFs in BMLS
    *** partially reqs: Raf1 [KO]
    *** source: 11296228-Fig-6a
  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (10 min)
    *** cells: mEFs in BMLS
    *** does not req: Raf1 [KO]
    *** source: 11296228-Fig-6d
  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0 2+++ 5+++ 15++ 45+ min
    *** does not req: Raf1 [KO]
    *** source: 11296228-Fig-6e





Mek1 features

  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased irt Egf (10 min)
    *** cells: rEFs in BMLS
    *** unaffected by: xMek1(K97A)"KD" [substitution]
    *** unaffected by: xMek1(S298A) [substitution]
    *** source: 17314031-Fig-2b
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased irt Egf (20 min)
    *** cells: REF52 in BMS "in suspension"
    *** unaffected by: xMek1(S298A) [substitution]
    *** source: 12876277-Fig-10a
  *** Mek12s[phosAb] phos(sitenr)[phosAb] is increased irt Egf (5 min)
    *** cells: PC6 in BMLS
    *** source: 15632082-Fig-1c
  *** xMek1[phosAb] phos(SMANS)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs<Ksr1~null><xKsr1><xMek1> in BMLS
    *** inhibited by: xMek1(M308A/I310A) [substitution]
    *** source: 19541618-Fig-2d
  *** xMek1[phosAb] phos(SMANS)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs<Ksr1~null><xMek1> in BMLS
    *** inhibited by: xMek1(M308A/I310A) [substitution]
    *** source: 19541618-Fig-2d

chemicals

  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (times)
    *** cells: HELA in BMS
    *** times: 0 2+ 5+ 10+ min
    *** unaffected by: BaptaAM [addition]
    *** source: 16858395-Fig-5e
  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (times)
    *** cells: HEK293 in BMLS
    *** times: 0 1+ 3+++ 4+++ 5+++ 10++ 20+ 30+ min
    *** partially inhibited by: Wortmannin [addition]
    *** source: 16687399-Fig-8c
  *** Mek1[Ab]IP IVKA(Erk2)[32P-ATP] is increased irt Egf (5 min)
    *** cells: HELA in BMLS
    *** unaffected by: Forskolin + IBMX [addition]
    *** source: 16581779-Fig-3b
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Egf (10 min)
    *** cells: H197 in BMS
    *** unaffected by: Rottlerin [pretreatment]
    *** unaffected by: Chelerythrine [pretreatment]
    *** unaffected by: Go6976 [pretreatment]
    *** unaffected by: PD98059 [pretreatment]
    *** source: 10330161-Fig-9a
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Egf (10 min)
    *** cells: PC12 in BMS
    *** unaffected by: Rottlerin [pretreatment]
    *** unaffected by: Go6976 [pretreatment]
    *** unaffected by: Chelerythrine [pretreatment]
    *** unaffected by: PD98059 [pretreatment]
    *** source: 10330161-Fig-9c
  *** Mek12s[phosAb] phos(sitenr)[phosAb] is increased irt Egf (30 min)
    *** cells: 3Y1<xEgfR> in BMS
    *** unaffected by: 1Butanol [pretreatment]
    *** unaffected by: isoButanol [pretreatment]
    *** source: 11134345-Fig-8c

no extras

  *** Mek12s[phosAb] phos(S218/S222)[phosAb] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** source: 16093354-Fig-7a
  *** xMek1[tAb]IP phos(S218/S222)[phosAb] is increased irt Egf (15 min)
    *** cells: COS7 in BMLS
    *** source: 16093354-Fig-7a
  *** xMek1[tAb]IP phos(SMANS)[phosAb] is increased irt Egf (5 min)
    *** cells: mEFs<Ksr1~null><xKsr1><xMek1> in BMLS
    *** source: 19541618-Fig-S2
  *** Mek12s[phosAb] phos(SMANS)[phosAb] is increased irt Egf (times)
    *** cells: A431 in BMLS
    *** times: 0 1 3++ 5++ 7++ 10++ 15+ 20++ 30+ min
    *** source: 19766101-Fig-2b


========
Location
========

  *** xMek?[XFP] locatedin(nucleus)[IHC] is unchanged irt Egf (10 min)
    *** cells: HELA<xMek?> in BMS
    *** source: 15239952-Fig-5b

=====================
Physical interactions
=====================

Braf--

  *** Mek12s[Ab] copptby[WB] Braf[tAb] is increased irt Egf (5 min)
    *** cells: mEFs<Ksr1~null><xKsr1> in BMLS
    *** reqs: xKsr1 [omission]
    *** source: 19541618-Fig-2b

Hras--

  *** Mek1[Ab] snaggedby[WB] rHras{GMPPNP}[GST] is increased irt Egf (20 min)
    *** cells: PC12 in BMS
    *** inhibited by: rHras{GDP} [substitution]
    *** source: 9001246-Fig-3b

IqGap1--

  *** xMek1[Ab] copptby[WB] IqGap1[Ab] is increased irt Egf (10 min)
    *** cells: MCF7 in BMLS
    *** source: 16135787-Fig-2a

Ksr1--

  *** Mek12s[Ab] copptby[WB] xKsr1[tAb] is detectable-but unchanged irt Egf (5 min)
    *** cells: mEFs<Ksr1~null><xKsr1><xMek1> in BMLS
    *** source: 19541618-Fig-3a
  *** Mek12s[Ab] copptby[WB] Ksr1[Ab] is detectable-but unchanged irt Egf (times)
    *** cells: mEFs in BMLS
    *** times: 0+ 5+ 20+ min
    *** source: 19541618-Fig-4c

Raf1--

  *** Mek12s[Ab] copptby[WB] Raf1[tAb] is unchanged irt Egf (5 min)
    *** cells: mEFs<Ksr1~null><xKsr1> in BMLS
    *** unaffected by: xKsr1 [omission]
    *** source: 19541618-Fig-2b

Rheb--

  *** Mek1[Ab] snaggedby[WB] rRheb{GMPPNP}[GST] is unchanged irt Egf (20 min)
    *** cells: PC12 in BMS
    *** unaffected by: rRheb{GDP} [substitution]
    *** source: 9001246-Fig-3b

=============
Other Effects
=============

  *** Mek1[phosAb] phos(S298)[phosAb] is unchanged irt Egf (5 min)
    *** cells: rEFs in BMLS
    *** source: 17314031-Fig-3
  *** xMek1[tAb]IP phos(S298)[phosAb] is unchanged irt Egf (10 min)
    *** cells: rEFs in BMLS
    *** source: 17314031-Fig-2b
  *** xMek1[tAb]IP phos(S298)[phosAb] is unchanged irt Egf (20 min)
    *** cells: REF52 in BMS "in suspension"
    *** unaffected by: xMek1(S298A) [substitution]
    *** source: 12876277-Fig-10a

  *** xMek1[tAb]IP phos(sitenr)[phosAb] is increased irt Egf (10 min)
    *** cells: COS7 in BMLS
    *** source: 12738796-Fig-2d
  *** xMek1[tAb]IP phos(sitenr)[phosAb] is increased irt Egf (10 min)
    *** cells: COS7 in BMLS
    *** source: 12738796-Fig-4c
