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Activation of Rac1 by ArhGef4 requires binding of Apc-Arm-domain
to overcome autoinhibition of ArhGef4 by its SH3 domain
see 17190834-Fig-S3 for cartoon of mechanism
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  *** xRac1[tAb] GTP-association[BDPD] is increased itpo xArhGef4
    *** cells: COS7 in BMS
    *** enhanced by: xArhGef4(R178A) [substitution]
    *** enhanced by: xArhGef4(E365A) [substitution]
    *** enhanced by: xArhGef4(W132L/D133A) [substitution]
    *** enhanced by: xArhGef4(D133A/E365A) [substitution]
    *** unaffected by: xArhGef4(66-540) [substitution]
    *** source: 17190834-Fig-2c


  *** rRac1 GDP-dissociation[3H-GDP] is increased by (rArhGef4 + rApc(mnr)"Arm-domain")
    *** cells: none
    *** reqs: both [omission]
    *** source: 10947987-Fig-3b
  *** rRac1 GDP-dissociation[3H-GDP] is increased by (rArhGef4 + rApc(mnr)"Arm-domain")
    *** cells: none
    *** reqs: rApc(mnr)"Arm-domain" [omission]
    *** source: 10947987-Fig-3c
  *** rRac1 GDP-dissociation[3H-GDP] is increased by (rArhGef4 + rApc(mnr)"Arm-domain")
    *** cells: none
    *** reqs: rApc(mnr)"Arm-domain" [omission]
    *** source: 10947987-Fig-3d
  *** rRac1 GDP-dissociation[3H-GDP] is increased by rArhGef4(mnr)"APC-binding-mutant"
    *** cells: none
    *** source: 10947987-Fig-3b
  *** rRac1 GDP-dissociation[3H-GDP] is increased by rArhGef4(mnr)"APC-binding-mutant"
    *** cells: none
    *** unaffected by: rApc(mnr)"Arm-domain" [addition]
    *** source: 10947987-Fig-3d

  *** rRac1[Ab] boundby[WB] rArhGef4(mnr)"APC-binding-mutant"[Resin] is detectable
    *** cells: none
    *** source: 10947987-Fig-3a



  *** rRac1 GTP-association[Mant-GTP] is unchanged by rArhGef4(271-632)"DH/PH"
    *** cells: none
    *** source: 17704816-Fig-1b
  *** rRac1 GTP-association[Mant-GTP] is unchanged by rArhGef4(271-632)"DH/PH"
    *** cells: none
    *** source: 17704816-Fig-S1
  *** rRac1 GDP-dissociation[Mant-GDP] is unchanged by rArhGef4(233-448)"DH"
    *** cells: none
    *** source: 17214551-Fig-2a

  *** rRac1 GTP-association[Mant-GTP] is increased by rArhGef4(271-632/L421I)"DH/PH"
    *** cells: none
    *** inhibited by: rArhGef4(271-632)"DH/PH" [substitution]
    *** source: 17704816-Fig-1b
