==========================
RagAB - RagCD heterodimers
==========================

  *** RagA[Ab] copptby[WB] RagC[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 20381137-Fig-2f
  *** xRagA[tAb] copptby[WB] xRagC[tAb] is detectable
    *** cells: BHK21<xRagA><xRagC> in BMS
    *** unaffected by: xRagA(T21L) [substitution]
    *** unaffected by: xRagA(Q66L) [substitution]
    *** source: 11073942-Fig-4b
  *** xRagA[tAb] copptby[WB] xRagD[tAb] is detectable
    *** cells: BHK21<xRagA><xRagD> in BMS
    *** inhibited by: xRagA(T21L) [substitution]
    *** unaffected by: xRagA(Q66L) [substitution]
    *** source: 11073942-Fig-4b
  *** xRagB[Ab] copptby[WB] xRagD[tAb] is detectable
    *** cells: HEK293T<xRagB><xRagD> in BMS
    *** source: 20381137-Fig-2e
  *** xRagC[tAb] copptby[WB] xRagA[tAb] is detectable
    *** cells: BHK21<xRagA><xRagC> in BMS
    *** source: 11073942-Fig-3
  *** xRagC[tAb] copptby[WB] xRagB[tAb] is detectable
    *** cells: BHK21<xRagB><xRagC> in BMS
    *** source: 11073942-Fig-3
  *** xRagD[tAb] copptby[WB] xRagA[tAb] is detectable
    *** cells: BHK21<xRagA><xRagD> in BMS
    *** source: 11073942-Fig-3
  *** xRagD[tAb] copptby[WB] xRagB[tAb] is detectable
    *** cells: BHK21<xRagB><xRagD> in BMS
    *** source: 11073942-Fig-3

=================================
RagAB - RagCD Direct interactions
=================================

  *** rRagA(161-208)[35S] boundby[WB] rRagC(230-399)[GST] is detectable
    *** cells: none
    *** source: 11073942-Fig-5d
  *** rRagC(230-399)[35S] boundby[WB] rRagA(161-208)[GST] is detectable
    *** cells: none
    *** source: 11073942-Fig-5d


==========================
Rag Atp6V1 coprecipitation
==========================

  *** RagA[Ab] copptby[WB] xAtp6V1b1[tAb] is decreased irt AminoAcids (15 min)
    *** cells: HEK293T in BMS
    *** source: 22053050-Fig-S4d


=========================================
Rag Lamtor coprecipitation/colocalization
=========================================

  *** RagAB[Ab] colocwith[IHC] xLamtor1[XFP] is detectable
    *** cells: HEK293T<xLamtor1> in BMS
    *** source: 20381137-Fig-2j

  *** RagA[tAb] copptby[WB] xLamtor2[tAb] is detectable
    *** cells: HEK293T<xLamtor2><xLamtor3><xLamtor1> in BMS
    *** source: 20381137-Fig-2d
  *** xRagB(Q99L)"GTP"[tAb] copptby[WB] xLamtor2[tAb] is detectable
    *** cells: HEK293T<xLamtor2><xLamtor3><xLamtor1><xMtor><xRaptor><xRagB(Q99L)"GTP"><xRagD(S77L)"GDP"> in BMS
    *** source: 20381137-Fig-2c
  *** RagB[tAb] copptby[WB] xLamtor2[tAb] is detectable
    *** cells: HEK293T<xLamtor2><xLamtor3><xLamtor1> in BMS
    *** source: 20381137-Fig-2d
  *** xRagB[tAb] copptby[WB] xLamtor2[tAb] is detectable
    *** cells: HEK293T<xLamtor2><xLamtor3><xLamtor1><xRagB><xRagD> in BMS
    *** source: 20381137-Fig-2b

  *** RagC[Ab] colocwith[IHC] xLamtor1[XFP] is detectable
    *** cells: HEK293T<xLamtor1> in BMS
    *** source: 20381137-Fig-2j
  *** RagC[tAb] copptby[WB] xLamtor2[tAb] is detectable
    *** cells: HEK293T<xLamtor2><xLamtor3><xLamtor1> in BMS
    *** source: 20381137-Fig-2d

  *** xRagD(S77L)"GDP"[tAb] copptby[WB] xLamtor2[tAb] is detectable
    *** cells: HEK293T<xLamtor2><xLamtor3><xLamtor1><xMtor><xRaptor><xRagB(Q99L)"GTP"><xRagD(S77L)"GDP"> in BMS
    *** source: 20381137-Fig-2c
  *** xRagD[tAb] copptby[WB] xLamtor2[tAb] is detectable
    *** cells: HEK293T<xLamtor2><xLamtor3><xLamtor1><xRagB><xRagD> in BMS
    *** source: 20381137-Fig-2b

  *** Lamtor1[Ab] copptby[WB] xRagD[tAb] is detectable
    *** cells: HEK293T<xRagB><xRagD> in BMS
    *** source: 20381137-Fig-2e
  *** Lamtor2[Ab] copptby[WB] RagC[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 20381137-Fig-2f
  *** Lamtor2[Ab] copptby[WB] xRagD[tAb] is detectable
    *** cells: HEK293T<xRagB><xRagD> in BMS
    *** source: 20381137-Fig-2e
  *** Lamtor3[Ab] copptby[WB] RagC[tAb] is detectable
    *** cells: HEK293T in BMS
    *** source: 20381137-Fig-2f
  *** Lamtor3[Ab] copptby[WB] xRagD[tAb] is detectable
    *** cells: HEK293T<xRagB><xRagD> in BMS
    *** source: 20381137-Fig-2e

=======================================================
Rag Lamtor coprecipitation is unchanged irt Amino Acids
=======================================================

  *** Lamtor1[Ab] copptby[WB] RagAB[Ab] is detectable-but unchanged irt AminoAcids (10 min)
    *** cells: HEK293T in BMS "AminoAcid-deprived"
    *** source: 20381137-Fig-2h
  *** Lamtor1[Ab] copptby[WB] xRagB[tAb] is detectable-but unchanged irt AminoAcids (10 min)
    *** cells: HEK293T<xRagB> in BMS "AminoAcid-deprived"
    *** partially inhibited by: xRagB(Q99L)"GTP" [substitution]
    *** source: 20381137-Fig-2i
  *** Lamtor2[Ab] copptby[WB] RagAB[Ab] is detectable-but unchanged irt AminoAcids (10 min)
    *** cells: HEK293T in BMS "AminoAcid-deprived"
    *** source: 20381137-Fig-2h
  *** Lamtor2[Ab] copptby[WB] xRagB[tAb] is detectable-but unchanged irt AminoAcids (10 min)
    *** cells: HEK293T<xRagB> in BMS "AminoAcid-deprived"
    *** partially inhibited by: xRagB(Q99L)"GTP" [substitution]
    *** source: 20381137-Fig-2i
  *** Lamtor3[Ab] copptby[WB] xRagB[tAb] is detectable-but unchanged irt AminoAcids (10 min)
    *** cells: HEK293T<xRagB> in BMS "AminoAcid-deprived"
    *** partially inhibited by: xRagB(Q99L)"GTP" [substitution]
    *** source: 20381137-Fig-2i

========================
Colocalizaton with Lamp2
========================

  *** xRagB(Q99L)"GTP"[Ab] colocwith[IHC] Lamp2[Ab] is detectable-but unchanged irt AminoAcids (10 min)
    *** cells: HEK293T<xRagB(Q99L)"GTP"> in BMS "AminoAcid-deprived"
    *** unaffected by: SalicylihalamideA [pretreatment]
    *** source: 22053050-Fig-2d
  *** RagC[Ab] colocwith[IHC] Lamp2[Ab] is detectable-but unchanged irt AminoAcids (10 min)
    *** cells: HEK293T in BMS "AminoAcid-deprived"
    *** unaffected by: SalicylihalamideA [cotreatment]
    *** source: 22053050-Fig-2c

===============================
RagB does not coppt with Atp6V0
===============================

  *** Atp6V0c[Ab] copptby[WB] xRagB[tAb] is undetectable
    *** cells: HEK293T<xRagB> in BMS
    *** source: 22053050-Fig-S4b
  *** Atp6V0d1[Ab] copptby[WB] xRagB[tAb] is undetectable
    *** cells: HEK293T<xRagB> in BMS
    *** source: 22053050-Fig-S4b

==========================
Rag Atp6V1 coprecipitation
==========================

  *** Atp6V1b2[Ab] copptby[WB] xRagB[tAb] is detectable
    *** cells: HEK293T<xRagB> in BMS
    *** source: 22053050-Fig-S4a
  *** Atp6V1d[Ab] copptby[WB] xRagB[tAb] is detectable
    *** cells: HEK293T<xRagB> in BMS
    *** source: 22053050-Fig-S4a

====================================
Rag Atp6V1 interaction is not direct
====================================

  *** xRagA[tAb]IP boundby[WB] xAtp6V1d[GST]IP is undetectable
    *** cells: none
    *** IPfrom: HEK293T<xRagC> in BMS
    *** IPfrom: HEK293T<xAtp6V1d> in BMS
    *** source: 22053050-Fig-S4c
  *** xRagC[tAb]IP boundby[WB] xAtp6V1d[GST]IP is undetectable
    *** cells: none
    *** IPfrom: HEK293T<xRagC> in BMS
    *** IPfrom: HEK293T<xAtp6V1d> in BMS
    *** source: 22053050-Fig-S4c

======================================================
Rag Atp6V1 coprecipitation is decreased irt AminoAcids
======================================================

  *** RagC[Ab] copptby[WB] xAtp6V1b1[tAb] is decreased irt AminoAcids (15 min)
    *** cells: HEK293T in BMS
    *** source: 22053050-Fig-S4d



























