======================
Direct phosphorylation
======================

  *** rS6k1(1-420)[32P] phos[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** comment: phos does not req PIP3 in kinse buffer
    *** source: 9427642-Fig-4a
  *** rS6k1(1-420)[32P] phos[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** comment: S6k1 phos-site was determined to be T252 by HPLC
    *** source: 9427642-Fig-5
  *** xS6k1(1-364)[tAb]IP phos(T252)[phosAb] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-364)> in BMS
    *** unaffected by: rS6k1(1-364/R121A) [substitution]
    *** source: 12374740-Fig-6b
  *** rS6k1(1-421/T412E)[GST] phos[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** inhibited by: rS6k1(1-421) [substitution]
    *** source: 12374740-Fig-4
  *** rS6k1(1-421/T412E)[GST] phos[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** partially inhibited by: rPdpk1(R131A) [substitution]
    *** partially inhibited by: rPdpk1(R131D) [substitution]
    *** source: 12374740-Fig-4

===========================
Semi-Direct Phosphorylation
===========================

  *** xS6k1(1-421)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-421)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2a
  *** xS6k1(1-421)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-421)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3a

=================
Direct Activation
=================

  *** rS6k1[GST] IVKA(auto)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** source: 9427642-Fig-6
  *** rS6k1[GST] IVKA(peptide)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** source: 9427642-Fig-6
  *** xS6k1[tAb]IP IVKA(Rps6)[32P-ATP] is increased itpo xPdpk1
    *** cells: CHO<xInsR> in BMLS
    *** source: 9427642-Fig-1b
  *** xS6k1(1-364)[tAb]IP IVKA(S6peptide)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-364)> in BMS
    *** enhanced by: pHMS6K [addition]
    *** unaffected by: HMS6K [addition]
    *** source: 12374740-Fig-6b
  *** xS6k1(1-364)[tAb]IP IVKA(S6peptide)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-364)> in BMS
    *** unaffected by: rS6k1(1-364/R121A) [substitution]
    *** source: 12374740-Fig-6b

=================
Knockout evidence
=================

  *** S6k1[Ab] phos[WBMS] is detectable
    *** cells: mESCs in BMLS
    *** partially reqs: Pdpk1 [KO]
    *** source: 11500364-Fig-S1c
  *** S6k1[phosAb] phos(T412)[phosAb] is detectable
    *** cells: mESCs in BMLS
    *** reqs: Pdpk1 [KO]
    *** source: 11500364-Fig-S1d
  *** S6k1[phosAb] phos(T444/T424)[phosAb] is detectable
    *** cells: mESCs in BMLS
    *** reqs: Pdpk1 [KO]
    *** source: 11500364-Fig-S1d
  *** S6k1[phosAb] phos(T412)[phosAb] is detectable
    *** cells: mESCs in BMLS
    *** inhibited by: kPdpk1(L155E)"PIF" [knockin]
    *** source: 16763566-Fig-3b
  *** S6k1[phosAb] phos(T412)[phosAb] is detectable
    *** cells: mESCs in BMLS
    *** reqs: Pdpk1 [KO]
    *** source: 16763566-Fig-3a

========================================================
S6k1 is not phosed on T252 by Pdpk1 until T412 is phosed
========================================================

  *** xS6k1(1-421)[GST]IP phos(T252)[phosAb] is unchanged by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-421)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3a
  *** xS6k1(1-421)[GST]IP phos(T252)[phosAb] is unchanged by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-421)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** unaffected by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2a

  *** xS6k1(1-421/T412E)[GST]IP phos(T252)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-421/T412E)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2a
  *** xS6k1(1-421/T412E)[GST]IP phos(T252)[phosAb] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-421/T412E)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3a
  *** xS6k1(1-421/T412E)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-421/T412E)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-2a
  *** xS6k1(1-421/T412E)[GST]IP phos[32P-ATP] is increased by xPdpk1[GST]IP
    *** cells: none
    *** IPfrom: HEK293<xS6k1(1-421/T412E)> in BMS
    *** IPfrom: HEK293<xPdpk1> in BMS
    *** inhibited by: PIFtide [addition]
    *** source: 11500365-Fig-3a

===================
Protein Association
===================

  *** xS6k1(1-421)[tAb] copptby[WB] xPdpk1[GST] is detectable
    *** cells: HEK293<xPdpk1><xS6k1(1-421)> in BMS
    *** enhanced by: xS6k1(1-421/S412E) [substitution]
    *** inhibited by: xS6k1(S412E) [substitution]
    *** inhibited by: xS6k1(WT) [substitution]
    *** source: 11500365-Fig-5a
  *** xS6k1(1-421)[tAb] copptby[WB] xPdpk1[GST] is detectable
    *** cells: HEK293<xPdpk1><xS6k1(1-421)> in BMS
    *** inhibited by: xPdpk1(L155E) [substitution]
    *** source: 11500365-Fig-5a










