===============
Kinase Activity
===============

requires Mek1--

  *** xRsk1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk1> in BMLS
    *** inhibited by: xMek1(S218A) [substitution]
    *** unaffected by: xMek1(S218E/S222E) [substitution]
    *** source: 8939914-Fig-2

not Msk1--

  *** Rsk1[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt Egf (times)
    *** cells: mESCs in BMLS
    *** times: 0 10+ 20 30 min
    *** does not req: Msk1 [KO]
    *** source: 11018520-Fig-4a

Rsk1 features

  *** xRsk1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** inhibited by: xRsk1(1-721) [substitution]
    *** inhibited by: xRsk1(L722A) [substitution]
    *** inhibited by: xRsk1(R725A) [substitution]
    *** inhibited by: xRsk1(R726A) [substitution]
    *** unaffected by: xRsk1(K728A) [substitution]
    *** unaffected by: xRsk1(K729A) [substitution]
    *** unaffected by: xRsk1(L730A) [substitution]
    *** unaffected by: xRsk1(S732A) [substitution]
    *** source: 12832467-Fig-2b
  *** xRsk1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** inhibited by: xRsk1(K94R) [substitution]
    *** partially inhibited by: xRsk1(K447R) [substitution]
    *** inhibited by: xRsk1(K94R/K447R) [substitution]
    *** source: 12832467-Fig-5a
  *** xRsk1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (times)
    *** cells: HEK293<xRsk1> in BMLS
    *** times: 0 10+++ 30++ 60+ 90+ 120+ min
    *** unaffected by: xRsk1(S732A) [substitution]
    *** source: 12832467-Fig-7b
  *** xRsk1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk1> in BMLS
    *** source: 10480933-Fig-5

Chemicals

  *** Rsk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt Egf (10 min)
    *** cells: RAT2 in BMS
    *** unaffected by: H89 [addition]
    *** source: 11297520-Fig-7b
  *** Rsk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt Egf (times)
    *** cells: RAT2 in BMS
    *** times: 0 2+ 5++ 10++ 20++ 40+ min
    *** inhibited by: PD184352 [addition]
    *** source: 11297520-Fig-5a
  *** xRsk1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293E<xRsk1> in BMLS
    *** inhibited by: U0126 [pretreatment]
    *** source: 12906785-Fig-1c
  *** xRsk1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk1> in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** source: 8939914-Fig-3b

No extras

  *** xRsk1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** source: 12832467-Fig-6c
  *** xRsk1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (times)
    *** cells: HEK293<xRsk1> in BMLS
    *** times: 0 10+++ 30++ 60+ 90+ 120+ min
    *** source: 12832467-Fig-7a
  *** xRsk1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt Egf (15 min)
    *** cells: COS7<xRsk1> in BMLS
    *** source: 8939914-Fig-1b

===================
Rsk1(phosT359/S363)
===================

  *** Rsk1[phosAb] phos(T359/S363)[phosAb] is increased irt Egf (5 min)
    *** cells: 3731T in BMLS
    *** source: 19274086-Fig-2a
  *** Rsk1[phosAb] phos(T359/S363)[phosAb] is increased irt Egf (5 min)
    *** cells: MEWO in BMLS
    *** source: 19274086-Fig-2a
  *** Rsk1[phosAb] phos(T359/S363)[phosAb] is unchanged irt Egf (5 min)
    *** cells: B16F1 in BMLS
    *** source: 19274086-Fig-2a

==============
Rsk1(phosS380)
==============

No extras

  *** Rsk1[phosAb] phos(S380)[phosAb] is increased irt Egf (times)
    *** cells: MDAMB361 in BMLS
    *** times: 0 5++ 10++ 20++ 30++ 45++ 60+ min
    *** source: 11297548(D)

==============
Rsk1(phosT573)
==============

Erk2--

  *** xRsk1[phosAb] phos(T573)[phosAb] is increased irt Egf (5 min)
    *** cells: HELA<Erk1~null><Erk2~null><xErk2><xRsk1> in BMLS
    *** reqs: xErk2 [omission]
    *** partially inhibited by: xErk2(L232A) [substitution]
    *** partially inhibited by: xErk2(Y261A) [substitution]
    *** inhibited by: xErk2(D319N) [substitution]
    *** comment: xRsk1 is actually "avian Rsk"
    *** source: 16051177-Fig-3a

  *** xRsk1[tAb]IP phos(T573)[phosAb] is increased irt Egf (10 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** reversed by: U0126 [pretreatment]
    *** source: 12832467-Fig-1b
  *** xRsk1[tAb]IP phos(T573)[phosAb] is increased irt Egf (times)
    *** cells: HEK293<xRsk1> in BMLS
    *** times: 0 2++ 5++ 10++ 30++ 60+ min
    *** source: 12832467-Fig-1a
  *** xRsk1[phosAb] phos(T573)[phosAb] is increased irt Egf (5 min)
    *** cells: HELA<xRsk1> in BMLS
    *** comment: xRsk1 is actually "avian Rsk"
    *** source: 16051177-Fig-3a

==========================
Coprecipitation with Erks
==========================

  *** Erks[Ab] copptby[WB] xRsk1[tAb] is decreased irt Egf (10 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** inhibited by: xRsk1(mnr)"KD" [substitution]
    *** source: 12832467-Fig-5c
  *** Erks[Ab] copptby[WB] xRsk1[tAb] is decreased irt Egf (10 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** reversed by: U0126 [pretreatment]
    *** source: 12832467-Fig-1b
  *** Erks[Ab] copptby[WB] xRsk1[tAb] is decreased irt Egf (10 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** reversed by: xRsk1(S732A) [substitution]
    *** source: 12832467-Fig-2b
  *** Erks[Ab] copptby[WB] xRsk1[tAb] is decreased irt Egf (10 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** source: 12832467-Fig-1c
  *** Erks[Ab] copptby[WB] xRsk1[tAb] is decreased irt Egf (10 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** source: 12832467-Fig-6c
  *** Erks[Ab] copptby[WB] xRsk1[tAb] is decreased irt Egf (10 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** unaffected by: xRsk1(K94R) [substitution]
    *** unaffected by: xRsk1(K447R) [substitution]
    *** reversed by: xRsk1(K94R/K447R) [substitution]
    *** source: 12832467-Fig-5a
  *** Erks[Ab] copptby[WB] xRsk1[tAb] is decreased irt Egf (times)
    *** cells: HEK293<xRsk1> in BMLS
    *** times: 0++ 2 5 10 30+ 60++ min
    *** source: 12832467-Fig-1a


  *** xRsk1[tAb]IP IVKA(Rps6)[32P-ATP] is increased irt Egf (10 min)
    *** cells: HEK293E<xRsk1> in BMLS
    *** unaffected by: xRheb [addition]
    *** source: 12906785-Fig-1c
