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Things that are required for increase in Erk phosphorylation irt IL1
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not Ikk1--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30 min
    *** does not req: Ikk1 [KO]
    *** comment: decrease in phos at 30 min is not seen in Ikk-null cells
    *** source: 11733537-Fig-2

not Ikk2--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30 min
    *** does not req: Ikk2 [KO]
    *** comment: decrease in phos at 30 min is not seen in Ikk-null cells
    *** source: 11733537-Fig-2

Irak1--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 5++ 15+++ 30++ 45+ 60+ min
    *** reqs: Irak1 [KO]
    *** source: 19754427-Fig-1a

Irak4--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 5+ 10+ 15+ 30 min
    *** unaffected by: kIrak4(K213A/K214A)"KD" [substitution]
    *** source: 17337443-Fig-3b

not Mekk1--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (times)
    *** cells: mESCs in BMS
    *** times: 0 10++ 30+ 60 min
    *** repressed by: Mekk1 [KO]
    *** source: 10805784-Fig-2

not Mekk3--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 10+ 20+ 40+ 60+ min
    *** does not req: Mekk3 [KO]
    *** source: 14661019(D)
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1a (times)
    *** cells: mEFs in BMLS
    *** times: 0 10++ 15++ 30+ min
    *** does not req: Mekk3 [KO]
    *** source: 18021073-Fig-8b

not Mnk1-- or Mnk2--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (15 min)
    *** cells: mEFs in BMLS
    *** does not req: Mnk1 or Mnk2 [DKO]
    *** source: 15254222-Fig-3a

not Nfkbiz--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 15+ 30 60 min
    *** does not req: Nfkbiz [KO]
    *** source: 15241416-Fig-S3b

not Ripk1--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 10++ 15+ min
    *** does not req: Ripk1 [KO]
    *** source: 12776182-Fig-1c

Tak1--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs in BMLS
    *** times: 0 2 5 10+ 30 min
    *** inhibited by: xTak1(mnr)"KD" [addition]
    *** source: 18180283-Fig-6a

not Tollip--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 15+++ 30++ 60+ 180++ min
    *** does not req: Tollip [KO]
    *** source: 16428431(D)

not Traf2--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (times)
    *** cells: mEFs in BMS
    *** times: 0 5++ 10++ 15+ min
    *** does not req: Traf2 [KO]
    *** source: 12776182-Fig-1c

Traf6--

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (times)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** times: 0 5 15+ 30+ min
    *** inhibited by: xTraf6(110-522)"T6dR" [substitution]
    *** unaffected by: xTraf6(C242A/C249A)"T6mZ5" [substitution]
    *** source: 19843958-Fig-S12e
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (times)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** times: 0 30+ 60 360 720 1440 min
    *** unaffected by: xTraf6(C242A/C249A)"T6mZ5" [substitution]
    *** source: 19843958-Fig-S12e
  *** xErk2[phosAb] phos(TEY)[phosAb] is increased irt IL1 (20 min)
    *** cells: HELA<xTraf6> in BMS
    *** source: 16371247-Fig-3d
  *** xErk2[phosAb] phos(TEY)[phosAb] is unchanged irt IL1 (20 min)
    *** cells: HELA<xTraf6(del(289-522))> in BMS
    *** source: 16371247-Fig-3d


Tpl2--  can Tpl2 substitute for Irak1???

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (20 min)
    *** cells: HELA in BMS
    *** reqs: Tpl2 [RNAi]
    *** source: 16371247-Fig-2c
  *** xErk2[phosAb] phos(TEY)[phosAb] is increased irt IL1 (20 min)
    *** cells: HELA in BMS
    *** reqs: Tpl2 [RNAi]
    *** source: 16371247-Fig-1c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: mEFs in BMS
    *** times: 0 10+ 20+ 30+ 45 60 min
    *** partially reqs: Tpl2 [KO]
    *** source: 15833743-Fig-1c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (15 min)
    *** cells: HEK293<xIL1R1><Irak1~null><xTpl2> in BMLS
    *** inhibited by: xTpl2(D270A) [substitution]
    *** inhibited by: xTpl2(K167R) [substitution]
    *** inhibited by: xTpl2((30-397)/D270A) [substitution]
    *** inhibited by: xTpl2((30-397)/K167R) [substitution]
    *** source: 19754427-Fig-6
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (15 min)
    *** cells: HEK293<xIL1R1><Irak1~null><xTpl2> in BMLS
    *** unaffected by: xTpl2(30-467) [substitution]
    *** unaffected by: xTpl2(30-397) [substitution]
    *** source: 19754427-Fig-4b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (15 min)
    *** cells: HEK293<xIL1R1><Irak1~null><xTpl2> in BMLS
    *** unaffected by: xTpl2(30-467) [substitution]
    *** unaffected by: xTpl2(30-397) [substitution]
    *** source: 19754427-Fig-5
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (15 min)
    *** cells: HEK293<xIL1R1><Irak1~null><xTpl2> in BMLS
    *** unaffected by: xTpl2(S62A) [substitution]
    *** bkg inhibited by: xTpl2(T290A) [substitution]
    *** unaffected by: xTpl2(S400A) [substitution]
    *** inhibited by: xTpl2(S62A/S400A) [substitution]
    *** source: 19754427-Fig-8b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (15 min)
    *** cells: HEK293<xIL1R1><Irak1~null><xTpl2><xTnip2> in BMLS
    *** inhibited by: xTpl2(D270A) [substitution]
    *** inhibited by: xTpl2(T290A) [substitution]
    *** source: 19754427-Fig-9a

=========
chemicals
=========

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1a (20 min)
    *** cells: KB in BMS
    *** unaffected by: SB203580 [addition] no-bkg-control
    *** inhibited by: PD184352 [addition] no-bkg-control
    *** source: 14592977-Fig-10
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (15 min)
    *** cells: HEK293<xIL1R1> in BMLS
    *** bkg inhibited by: PP2 [addition]
    *** source: 19754427-Fig-3b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (15 min)
    *** cells: HEK293<xIL1R1> in BMLS
    *** partially inhibited by: PS1145 [addition]
    *** source: 19754427-Fig-1c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (15 min)
    *** cells: HEK293<xIL1R1><Irak1~null><xTpl2> in BMLS
    *** unaffected by: PS1145 [addition]
    *** source: 19754427-Fig-2b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (15 min)
    *** cells: HEK293<xIL1R1><Irak1~null><xTpl2><xTnip2> in BMLS
    *** inhibited by: Compound-1 [addition]
    *** source: 19754427-Fig-9a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (20 min)
    *** cells: HELA in BMS
    *** inhibited by: HerbimycinA [addition]
    *** inhibited by: PP2 [addition]
    *** bkg inhibited by: PP1 [addition]
    *** source: 16371247-Fig-4a
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (20 min)
    *** cells: HELA in BMS
    *** inhibited by: SC514 [addition]
    *** source: 16371247-Fig-3c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (20 min)
    *** cells: HELA in BMS
    *** partially inhibited by: MG132 [addition]
    *** source: 16371247-Fig-3b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (20 min)
    *** cells: HMEC1 in BMS
    *** bkg inhibited by: PP1 [addition]
    *** source: 16371247-Fig-4e
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (20 min)
    *** cells: hSNFs in BMS
    *** bkg inhibited by: PP1 [addition]
    *** source: 16371247-Fig-4d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1a (30 min)
    *** cells: HEPG2 in BMLS "plated on collagen"
    *** unaffected by: Gefitinib [cotreatment]
    *** partially inhibited by: BMS345541 [cotreatment]
    *** inhibited by: PD325901 [cotreatment]
    *** unaffected by: PHA818637 [cotreatment]
    *** unaffected by: ZSTK474 [cotreatment]
    *** source: 19953085-Fig-S1

=========
no extras
=========

  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: mKidneyCells in BMS
    *** times: 0 15+ 30+ min
    *** source: 16737960-Fig-1d
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 2 5+ 10+++ 20++ 30+ 40 50 60 min
    *** source: 18180283-Fig-5c
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 10++ 30+ min
    *** source: 18180283-Fig-6b
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1><xUbiq> in BMLS
    *** times: 0 5+ 10++ 30+ min
    *** source: 18180283-Fig-3
  *** Erks[phosAb] phos(TEY)[phosAb] is increased irt IL1 (times)
    *** cells: HELA in BMS
    *** times: 0 5+ 15++ 30++ 45+ 60 90 min
    *** source: 16371247-Fig-3a


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Contradictions
==============

  *** Erks[Ab]IP IVKA(Elk1)[32P-ATP] is unchanged irt IL1 (times)
    *** cells: CHO in BMLS
    *** times: 0 15 30 60 min
    *** source: 9111305-Fig-5c
  *** Erks[Ab]IP IVKA(Elk1)[32P-ATP] is unchanged irt IL1 (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 15 30 60 min
    *** source: 9111305-Fig-6c
