===============
Kinase Activity
===============

not Hras--

  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (times)
    *** cells: CHO<xInsR> in BMS
    *** times: 0 5+ 30++ min
    *** unaffected by: xHras(S17N)"DN" [addition]
    *** source: 8626428-Fig-6c

not Irak1--

  *** xJnk1[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (15 min)
    *** cells: EL4 in BMS
    *** unaffected by: xIrak1 [addition]
    *** unaffected by: xIrak1(K239S)"KD" [addition]
    *** source: 10217414-Fig-3a

not Mekk3--

  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (tnr)
    *** cells: mEFs in BMS
    *** does not req: Mekk3 [KO]
    *** source: 14661019(D)

not Mekk4--

  *** xJnk1[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (20 min)
    *** cells: COS7 in BMLS
    *** unaffected by: xMekk4(K1371R)"DN" [addition]
    *** source: 9305639-Fig-10b

not Mkk7--

  *** xJnk1[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (15 min)
    *** cells: KB in BMS
    *** unaffected by: xMkk7(mnr)"KD" [addition]
    *** source: 9384583-Fig-6b

Mkk4--

  *** xJnk1[tAb]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (30 min)
    *** cells: mEFs<xJnk1> in BMS
    *** source: 17187786-Fig-4a
  *** xJnk1[tAb]IP IVKA(Jun)[32P-ATP] is unchanged irt Sorbitol (30 min)
    *** cells: mEFs<Mkk4~null><Mkk7~null><xJnk1> in BMS
    *** source: 17187786-Fig-4c
  *** xJnk1[tAb]IP IVKA(Jun)[32P-ATP] is unchanged irt Sorbitol (30 min)
    *** cells: mEFs<Mkk4~null><xJnk1> in BMS
    *** source: 17187786-Fig-4b

not Mlk3--

  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Sorbitol (times)
    *** cells: mEFs in BMS
    *** times: 0 5 15+ 30++ 45++ 60++ 90++ 120++ min
    *** does not req: Mlk3 [KO]
    *** source: 15831472-Fig-8b


chemicals

  *** Jnk1[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (5 min)
    *** cells: M1241<xCd40> in BMS
    *** unaffected by: BaptaAM [pretreatment]
    *** unaffected by: TPEN [pretreatment]
    *** source: 10748139-Fig-5c
  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (times)
    *** cells: CHO<xInsR> in BMS
    *** times: 0 5+ 30++ min
    *** unaffected by: PD98059 [addition]
    *** source: 8626428-Fig-5b

no extras

  *** Jnk1[Ab]IP IVKA(Atf2)[32P-ATP] is increased irt Sorbitol (times)
    *** cells: HELA in BMS
    *** times: 0+ 15++ 30+++ 60+ 120+ min
    *** source: 7535770-Fig-5
  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (times)
    *** cells: CHO<xInsR> in BMS
    *** times: 0 5+ 30++ min
    *** source: 8626428-Fig-3b
  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (times)
    *** cells: NIH3T3 in BMLS
    *** times: 0 10+ 30++ min
    *** source: 8653792-Fig-4a
  *** Jnks[Ab]IP IVKA(Jun)[32P-ATP] is increased irt Sorbitol (times)
    *** cells: NIH3T3L in BMS "differentiated into adipocytes"
    *** times: 0 5+ 30++ min
    *** source: 8626428-Fig-3b

===========================
Phosphorylation on TPY site
===========================


  *** Jnks[phosAb] phos(TPY)[phosAb] is increased irt Sorbitol (30 min)
    *** cells: KB in BMS
    *** enhanced by: SB203580 [addition] no-bkg-control
    *** unaffected by: PD184352 [addition] no-bkg-control
    *** source: 14592977-Fig-10

