==========================================
No evidence for Ikba:Rela coppt with IL1R1
==========================================
Coprecipitation with other proteins in IL1R1C requires:

not Ikk1--

  *** Ikba[Ab] copptby[WB] Ikk2[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** does not req: Ikk1 [KO]
    *** source: 14636585-Fig-2d
  *** Ikba[Ab] copptby[WB] Mekk3[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** does not req: Ikk1 [KO]
    *** source: 14636585-Fig-2c

Ikk2--

  protein expression

  *** Ikba[Ab] copptby[WB] Ikk1[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Ikk2 [KO]
    *** source: 14636585-Fig-2f
  *** Ikba[Ab] copptby[WB] Mekk3[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Ikk2 [KO]
    *** source: 14636585-Fig-2c
  *** Rela[Ab] copptby[WB] Ikk1[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Ikk2 [KO]
    *** source: 14636585-Fig-2f

  phos sites but not kinase activity

  *** Ikba[Ab] copptby[WB] xIkk2[tAb] is increased irt IL1a (5 min)
    *** cells: mEFs<Ikk2~null><xIkk2> in BMS "on gelatin"
    *** unaffected by: xIkk2(K44M)"KD" [substitution]
    *** source: 14636585-Fig-4b
  *** Ikba[Ab] copptby[WB] xIkk2[tAb] is increased irt IL1a (5 min)
    *** cells: mEFs<Ikk2~null><xIkk2> in BMS "on gelatin"
    *** unaffected by: xIkk2(K44M)"KD" [substitution]
    *** inhibited by: xIkk2(K44M/S177A/S181A) [substitution]
    *** unaffected by: xIkk2(S177E/S181E) [substitution]
    *** source: 14636585-Fig-4c
  *** Rela[Ab] copptby[WB] xIkk2[tAb] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** inhibited by: xIkk2(K44M/S177A/S181A) [substitution]
    *** unaffected by: xIkk2(K44M)"KD" [substitution]
    *** source: 14636585-Fig-4c

Mekk3--  need evidence that Mekk3 must be ubiq

  protein expression

  *** Ikba[Ab] copptby[WB] Ikk1[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Mekk3 [KO]
    *** source: 14636585-Fig-2f
  *** Ikba[Ab] copptby[WB] Ikk2[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Mekk3 [KO]
    *** source: 14636585-Fig-2d
  *** Ikba[Ab] copptby[WB] Ikk2[Ab] is increased irt IL1a (times)
    *** cells: mEFs in BMS "on gelatin"
    *** times: 0 5+ 60 min
    *** reqs: Mekk3 [KO]
    *** source: 14636585-Fig-3b
  *** Ikk2[Ab] copptby[WB] Ikba[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Mekk3 [KO]
    *** source: 14636585-Fig-2a
  *** Ikba[Ab] copptby[WB] Ikk2[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Nemo [KO]
    *** source: 14636585-Fig-2d

  kinase activity

  *** Ikba[Ab] copptby[WB] xMekk3[tAb] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** source: 14636585-Fig-4a
  *** Rela[Ab] copptby[WB] xMekk3[tAb] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** source: 14636585-Fig-4a
  *** Ikk2[Ab] copptby[WB] xMekk3[tAb] is increased irt IL1a (5 min)
    *** cells: mEFs<Ikk2~null><xIkk2> in BMS "on gelatin"
    *** inhibited by: xMekk3(K391M)"KD" [substitution]
    *** source: 14636585-Fig-4a

Nemo-

  protein expression

  *** Ikba[Ab] copptby[WB] Mekk3[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Nemo [KO]
    *** source: 14636585-Fig-2c

no extras

  *** Mekk3[Ab] copptby[WB] Ikba[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** source: 14636585-Fig-2a
  *** Mekk3[Ab] copptby[WB] Rela[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** reqs: Ikba [KO]
    *** source: 14636585-Fig-2b

===================================================
Rela is missing some of Ikba's requirements because
it can use Ikbb and Mekk2 instead
===================================================

not Ikba--

  *** Ikk2[Ab] copptby[WB] Rela[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** does not req: Ikba [KO]
    *** source: 14636585-Fig-2b
  *** Rela[Ab] copptby[WB] Ikk1[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** does not req: Ikba [KO]
    *** source: 14636585-Fig-2f
  *** Rela[Ab] copptby[WB] Ikk2[Ab] is increased irt IL1a (times)
    *** cells: mEFs in BMS "on gelatin"
    *** times: 0 5+ 60+ min
    *** does not req: Ikba [KO]
    *** source: 14636585-Fig-2e

not Mekk3--

  *** Ikk2[Ab] copptby[WB] Rela[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** does not req: Mekk3 [KO]
    *** source: 14636585-Fig-2b
  *** Rela[Ab] copptby[WB] Ikk1[Ab] is increased irt IL1a (5 min)
    *** cells: mEFs in BMS "on gelatin"
    *** does not req: Mekk3 [KO]
    *** source: 14636585-Fig-2f
  *** Rela[Ab] copptby[WB] Ikk2[Ab] is increased irt IL1a (times)
    *** cells: mEFs in BMS "on gelatin"
    *** times: 0 5+ 60+ min
    *** does not req: Mekk3 [KO]
    *** source: 14636585-Fig-2e





