======================
Direct Phosphorylation
======================

  *** rBad[32P] phos[32P-ATP] is increased by rAkt1
    *** cells: none
    *** source: 11500364-Fig-3a
  *** rBad[32P] phos[32P-ATP] is increased by rAkt1
    *** cells: none
    *** source: 11297520-Fig-1a

===========================
Semi-Direct Phosphorylation
===========================

  *** rBad[32P] phos[32P-ATP] is increased by xAkt1[tAb]IP
    *** cells: none
    *** IPfrom: HEK293E<xAkt1> in BMLS "treated with Insulin for 20 min"
    *** inhibited by: Wortmannin [pretreatment] no-bkg-control
    *** source: 15024089-Fig-1c
  *** rBad[32P] phos[32P-ATP] is increased by xAkt1(myr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: BALB3T3<xAkt1(myr)"CA"> in BMLS
    *** inhibited by: xAkt1 [substitution]
    *** inhibited by: xAkt1(K179M)"KD" [substitution]
    *** source: 9346240-Fig-2a
  *** rBad[32P] phos[32P-ATP] is increased by xAkt1(myr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: BALB3T3<xAkt1(myr)"CA"> in BMLS
    *** unaffected by: rBad(S75A) [substitution]
    *** inhibited by: rBad(S99A) [substitution]
    *** inhibited by: rBad(S75A/S99A) [substitution]
    *** source: 9346240-Fig-2a
  *** rBad[32P] phos[32P-ATP] is increased by xAkt1(myr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: BALB3T3<xAkt1(myr)"CA"> in BMS
    *** inhibited by: xAkt1 [substitution]
    *** inhibited by: xAkt1(K179M)"KD" [substitution]
    *** source: 9346240-Fig-1a
  *** rBad[32P] phos[32P-ATP] is increased by xAkt1(myr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: COS7<xAkt1(myr)"CA"> in BMS
    *** inhibited by: xAkt1 [substitution]
    *** inhibited by: xAkt1(K179M)"KD" [substitution]
    *** source: 9346240-Fig-1a
  *** rBad[32P] phos[32P-ATP] is increased by xAkt1(myr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: HEK293<xAkt1(myr)"CA"> in BMS
    *** inhibited by: xAkt1 [substitution]
    *** inhibited by: xAkt1(K179M)"KD" [substitution]
    *** source: 9346240-Fig-1a

======================
Phosphorylation on S99
======================

  *** rBad[phosAb] phos(S99)[phosAb] is increased by xAkt1(myr)"CA"[tAb]IP
    *** cells: none
    *** IPfrom: BALB3T3<xAkt1(myr)"CA"> in BMLS
    *** unaffected by: rBad(S75A) [substitution]
    *** inhibited by: rBad(S99A) [substitution]
    *** inhibited by: rBad(S75A/S99A) [substitution]
    *** source: 9346240-Fig-3b
  *** xBad[tAb] phos(S99)[phosAb-ppt] is increased itpo xAkt1(myr)"CA"
    *** cells: HEK293<xBad> in BMS
    *** inhibited by: rBad(S99A) [substitution]
    *** inhibited by: rBad(S75A/S99A) [substitution]
    *** source: 9346240-Fig-4c
  *** xBad[tAb] phos(S99)[phosAb-ppt] is increased itpo xAkt1(myr)"CA"
    *** cells: HEK293<xBad> in BMS
    *** inhibited by: xAkt1(K179M)"KD" [substitution]
    *** source: 9346240-Fig-4c

===================
Protein Association
===================

  *** xAkt1[tAb] copptby[WB] xBad[Ab] is detectable
    *** cells: HEK293<xAkt1><xBad> in BMS
    *** unaffected by: rBad(S99A) [substitution]
    *** unaffected by: rBad(S75A/S99A) [substitution]
    *** source: 9346240-Fig-5b
  *** xAkt1[tAb] copptby[WB] xBad[Ab] is detectable
    *** cells: HEK293<xAkt1><xBad> in BMS
    *** unaffected by: xAkt1(K179M)"KD" [substitution]
    *** unaffected by: xAkt1(myr)"CA" [substitution]
    *** source: 9346240-Fig-5a

