=====================================================================
Smad2 relocation to nucleus requires phosphorylation on S465 and S467
=====================================================================

  *** Smad2[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** comment: Smad2 phos(S465/S467) is located in nuclear fraction
    *** source: 16751101-Fig-4d
  *** Smad2[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** comment: Smad2 in nucfraction is phosed on (S465/S467)[phosAb]
    *** comment: Smad2 in nucfraction is phosed on (S245/S250/S255)[phosAb]
    *** source: 17085434-Fig-1d
  -------------------------------------------------------------------------------
  *** Smad2[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS "treated with CHX"
    *** times: 0 30++ 60++ 120++ 180++ 360+ 540 min
    *** comment: Smad2 is phosed on S465/S467 [phosAb] during the times it is in the nuc fraction
    *** source: 11074002-Fig-7a
  *** Smad2[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (times)
    *** cells: NIH3T3 in BMS "treated with CHX"
    *** times: 0 30++ 60++ 120++ 180++ 360 540 min
    *** comment: Smad2 is phosed on S465/S467 [phosAb] during the times it is in the nuc fraction
    *** source: 11074002-Fig-7b
  -------------------------------------------------------------------------------
  *** xSmad2[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: MV1LU in BMS
    *** inhibited by: xSmad2(S464A/S465A/S467A) [substitution]
    *** source: 9346966-Fig-6
  *** xSmad2[tAb] locatedin(nucleus)[IHC] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: HEPG2<xSmad2> in BMS
    *** inhibited by: xSmad2(S464A/S465A/S467A) [substitution]
    *** source: 8980228-Fig-7


==================================================================
Smad2 release from TgfbR1 complex requires Smad2 phosphorylation
on S465 and S467 by TgfbR1
==================================================================

  *** xTgfbR1(K233R)"KD"[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR2> in BMS
    *** inhibited by: xTgfbR1(WT) [substitution]
    *** source: 9346966-Fig-5
  *** xTgfbR2[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR1(K233R)"KD"> in BMS
    *** inhibited by: xTgfbR1(WT) [substitution]
    *** source: 9346966-Fig-5
  *** xTgfbR1(mnr)"KD"[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR1(mnr)"KD"><xTgfbR2> in BMS "treated with Tgfb1 (30 min)"
    *** inhibited by: xTgfbR1 [substitution]
    *** source: 8980228-Fig-1a
  *** xTgfbR2(mnr)"KD"[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR1(mnr)"KD"><xTgfbR2> in BMS "treated with Tgfb1 (30 min)"
    *** inhibited by: xTgfbR1 [substitution]
    *** source: 8980228-Fig-1a
  *** xTgfbR1(mnr)"KD"[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR1(mnr)"KD"><xTgfbR2> in BMS "treated with Tgfb1 (30 min)"
    *** inhibited by: xTgfbR2(mnr)"KD"[tAb]IP [substitution]
    *** source: 8980228-Fig-1b
  *** xTgfbR2(mnr)"KD"[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR1(mnr)"KD"><xTgfbR2> in BMS "treated with Tgfb1 (30 min)"
    *** inhibited by: xTgfbR2(mnr)"KD"[tAb]IP [substitution]
    *** source: 8980228-Fig-1b
  *** xTgfbR1(mnr)"KD"[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR1(mnr)"KD"><xTgfbR2> in BMS "treated with Tgfb1 (30 min)"
    *** inhibited by: xTgfbR1 [substitution]
    *** source: 8980228-Fig-3a
  *** xTgfbR1(mnr)"KD"[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR1(mnr)"KD"><xTgfbR2><xSmad2> in BMS "treated with Tgfb1 (30 min)"
    *** inhibited by: xTgfbR1 [substitution]
    *** source: 8980228-Fig-6b

  *** xTgfbR1(K233R)[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR2> in BMS
    *** enhanced by: xSmad2(S464A) [substitution]
    *** enhanced by: xSmad2(S465A) [substitution]
    *** enhanced by: xSmad2(S465D) [substitution]
    *** enhanced by: xSmad2(S467A) [substitution]
    *** inhibited by: xSmad2(S467D) [substitution]
    *** enhanced by: xSmad2(S465A/S467A) [substitution]
    *** enhanced by: xSmad2(S464A/S465A/S467A) [substitution]
    *** unaffected by: xSmad2(S465A/S467D) [substitution]
    *** source: 9346966-Fig-5
  *** xTgfbR1[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR2> in BMS
    *** enhanced by: xSmad2(S464A) [substitution]
    *** enhanced by: xSmad2(S465A) [substitution]
    *** enhanced by: xSmad2(S465D) [substitution]
    *** enhanced by: xSmad2(S467A) [substitution]
    *** enhanced by: xSmad2(S467D) [substitution]
    *** enhanced by: xSmad2(S465A/S467A) [substitution]
    *** enhanced by: xSmad2(S464A/S465A/S467A) [substitution]
    *** enhanced by: xSmad2(S465A/S467D) [substitution]
    *** source: 9346966-Fig-5
  *** xTgfbR2[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR1(K233R)> in BMS
    *** enhanced by: xSmad2(S464A) [substitution]
    *** enhanced by: xSmad2(S465A) [substitution]
    *** enhanced by: xSmad2(S465D) [substitution]
    *** enhanced by: xSmad2(S467A) [substitution]
    *** inhibited by: xSmad2(S467D) [substitution]
    *** enhanced by: xSmad2(S465A/S467A) [substitution]
    *** enhanced by: xSmad2(S464A/S465A/S467A) [substitution]
    *** unaffected by: xSmad2(S465A/S467D) [substitution]
    *** source: 9346966-Fig-5
  *** xTgfbR2[125I-Tgfb1-XL] copptby[WB] xSmad2[tAb] is detectable
    *** cells: COS1<xTgfbR1> in BMS
    *** enhanced by: xSmad2(S464A) [substitution]
    *** enhanced by: xSmad2(S465A) [substitution]
    *** enhanced by: xSmad2(S465D) [substitution]
    *** enhanced by: xSmad2(S467A) [substitution]
    *** enhanced by: xSmad2(S467D) [substitution]
    *** enhanced by: xSmad2(S465A/S467A) [substitution]
    *** enhanced by: xSmad2(S464A/S465A/S467A) [substitution]
    *** enhanced by: xSmad2(S465A/S467D) [substitution]
    *** source: 9346966-Fig-5
  *** xTgfbR1[125I-Tgfb1-XL] copptby[WB] xSmad2(S464A/S465A/S467A)[tAb] is detectable
    *** cells: COS1<xTgfbR1(mnr)"KD"><xTgfbR2><xSmad2(S464A/S465A/S467A)> in BMS "treated with Tgfb1 (30 min)"
    *** inhibited by: xSmad2 [substitution]
    *** source: 8980228-Fig-6b

==================================================
Smad2 relocation to nucleus does not require Smad4
==================================================

  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: BXPC3<Smad4~null> in BMS "treated with CHX"
    *** times: 0 60++ 90++ 120++ 180++ 240+ min
    *** source: 12191474-Fig-5a
  *** Smad2[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (24 hr)
    *** cells: BXPC3<Smad4~null> in BMS
    *** source: 17638924-Fig-1
  *** Smad2[Ab] infraction(nuclear)[WB] is unchanged irt Tgfb1 (24 hr)
    *** cells: CAPAN1<Smad4~null> in BMS
    *** source: 17638924-Fig-1

==================================================================
Smad2 relocation to nucleus does not require new protein synthesis
==================================================================

  *** Smad2[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS "nuclear-extracts"
    *** times: 0 1 5 10+ 15+ 20++ 30++ 45++ 60++ 120++ 180+ 240+ 300+ 360 420 480 min
    *** comment: decrease in signal after 120 min is faster itpo of CHX
    *** source: 12191474-Fig-1a
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (60 min)
    *** cells: HACAT in BMS "treated with CHX"
    *** source: 12191474-Fig-5c
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (60 min)
    *** cells: HACAT in BMS "treated with CHX"
    *** unaffected by: NaN3 + 2DOG [addition]
    *** source: 12191474-Fig-6
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS "treated with CHX"
    *** times: 0 60++ 90++ 120++ 180++ 240+ min
    *** source: 12191474-Fig-3b
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS "treated with CHX"
    *** times: 0 1++ 4++ 9+ hr
    *** source: 11074002-Fig-6d

=========================
inhibition itpo of Smad7
=========================

  *** xSmad2[tAb] locatedin(nucleus)[IHC] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: HEPG2 in BMS
    *** inhibited by: xSmad7 [addition]
    *** source: 9215638-Fig-3b

===========================
inhibition itpo of Hras-GTP
===========================

  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (30 min)
    *** cells: EPH4 in BMS
    *** inhibited by: xHras(mnr)"vRas" [addition]
    *** source: 10197981-Fig-2d
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (30 min)
    *** cells: MV1LU in BMS
    *** inhibited by: Egf (30 min) [pretreatment]
    *** source: 10197981-Fig-6a
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: EPH4 in BMS
    *** times: 0 30++ 60++ 180++ 240+ min
    *** inhibited by: xHras(mnr)"vRas" [addition]
    *** source: 10197981-Fig-2e

=============
requires Dnm1
=============

  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (30 min)
    *** cells: HELA in BMS
    *** inhibited by: xDnm1(K44E)"DN" [addition]
    *** source: 12356868-Fig-5a

=====
other
=====

  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS
    *** times: 0 2 5 10+ 20+ 30+ 50+ min
    *** source: 12191474-Fig-3a
  *** Smad2[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: MV1LU in BMLS
    *** times: 0 2+ 5++ 15+++ min
    *** source: 17420258-Fig-2
  *** Smad2[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: MV1LU in BMLS
    *** times: 0 2+ 5++ 15+++ min
    *** source: 17420258-Fig-S1
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: COS1 in BMS
    *** times: 0+ 60++ 210+ min
    *** comment: decrease at 210 min is inhibited by Lactacystin [addition]
    *** source: 10587642-Fig-3a
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS
    *** times: 0+ 60++ 210+ min
    *** comment: decrease at 210 min is inhibited by Lactacystin [addition]
    *** source: 10587642-Fig-3a
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: HACAT in BMS
    *** times: 0 10+ 60+ min
    *** source: 11074002-Fig-6a
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (times)
    *** cells: MV1LU in BMS
    *** times: 0 15+ 30+ min
    *** source: 12356868-Fig-8a
  *** Smad2[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** source: 12150994-Fig-5a
  *** Smad2[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: MV1LU in BMS
    *** source: 9311995-Fig-7
  *** Smad2[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (30 min)
    *** cells: HEPG2 in BMS
    *** unaffected by: Wortmannin [addition]
    *** source: 12034739(D)
  *** Smad2[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (30 min)
    *** cells: MV1LU in BMS
    *** unaffected by: Nystatin [addition]
    *** source: 12717440-Fig-7c
  *** Smad2[Ab] infraction(nuclear)[WB] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** does not req: Ppm1a [RNAi]
    *** source: 16751101-Fig-4d
  *** Smad23s[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (30 min)
    *** cells: HELA in BMS
    *** source: 10934479-Fig-3a
  *** Smad2[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb1 (tnr)
    *** cells: AKR2B in BMLS
    *** unaffected by: LY294002 [pretreatment]
    *** source: 16288034-Fig-2b
  *** Smad2[Ab] locatedin(nucleus)[IHC] is increased irt Tgfb2 (30 min)
    *** cells: ATDC5 in BMLS
    *** unaffected by: U0126 [addition]
    *** unaffected by: SB203580 [addition]
    *** source: 11278290-Fig-5a
  -----------------------------------------------------------------------------
  *** xSmad2[tAb] locatedin(nucleus)[IHC] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS1 in BMS
    *** source: 9786930-Fig-5
  *** xSmad2[tAb] locatedin(nucleus)[IHC] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: MV1LU in BMS
    *** source: 9865696-Fig-4c

==============
unenlightening
==============

  *** xSmad2[tAb]IP infraction(nuclear)[WB] is increased irt Tgfb1 (tnr)
    *** cells: HEP3B in BMLS "treated with TrichostatinA"
    *** source: 17478422-Fig-1e
  *** xSmad2[MS2] nuc-export[NEreporter] is decreased irt Tgfb1 (24 hr)
    *** cells: HEK293T in BMS
    *** source: 12191473-Fig-7c


======================================================
Effect of acetyl on Smad2 location:
acetyl may prevent Smad2 from exiting the nucleus
======================================================

  *** xSmad2[tAb] locatedin(nucleus)[IHC] is increased irt Tgfb1 (1 hr)
    *** cells: HEP3B in BMLS "treated with TrichostatinA"
    *** inhibited by: xSmad2(K19R) [substitution]
    *** inhibited by: xSmad2(K20R) [substitution]
    *** inhibited by: xSmad2(K39R) [substitution]
    *** inhibited by: xSmad2(K19R/K20R) [substitution]
    *** inhibited by: xSmad2(K19R/K39R) [substitution]
    *** inhibited by: xSmad2(K20R/K39R) [substitution]
    *** inhibited by: xSmad2(K19R/K20R/K39R) [substitution]
    *** comment: xSmad2(K19Q/K20Q/K39Q) is located in the nucleus in untreated cells
    *** source: 17478422-Fig-7
















