  *** Rad50[Ab] snaggedby[WB] sH2ax(124-143){phos(S140)}[biotin] is detectable
    *** cells: HELA in BMS
    *** inhibited by: sH2ax(124-143) [substitution]
    *** source: 15201865-Fig-5b
  *** Rad50[Ab] snaggedby[WB] sH2ax(124-143){phos(S140)}[biotin] is detectable
    *** cells: HELA in BMS
    *** reqs: Mdc1 [RNAi]
    *** source: 15201865-Fig-5b

  *** Rad50[Ab] snaggedby[WB] sH2ax(128-142){phos(S140)}[biotin] is detectable
    *** cells: HEK293T in BMS "nuclear-extracts" "treated with IR for tnr"
    *** inhibited by: sH2ax(128-142){phos(S140/Y143)} [substitution]
    *** source: 19234442-Fig-5b


  *** Rad50[Ab] copptby[WB] xAtm[tAb] is detectable-but unchanged irt IR (1 hr)
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS
    *** source: 20026654-Fig-4a
  *** Rad50[Ab] copptby[WB] xAtm[tAb] is detectable-but unchanged irt IR (1 hr)
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 20026654-Fig-4b

====================
Rad50 dsDNA binding
====================

does not req Nbs1--

  *** rRad50[Ab] boundby[WB] dsDNA[biotin] is detectable
    *** cells: none
    *** comment: reaction also contains rMre11 + rNbs1
    *** comment: binding does not req rNbs1 [omission]
    *** unaffected by: xAtm{dimer}[tAb]IP [addition]
    *** source: 15790808-Fig-2d

requires Mre11--

  *** rRad50[Ab] boundby[WB] dsDNA[biotin] is detectable
    *** cells: none
    *** comment: reaction also contains rMre11 + rNbs1
    *** comment: binding reqs rMre11 and/or rNbs1 [omission]
    *** unaffected by: xAtm{dimer}[tAb]IP [addition]
    *** source: 15790808-Fig-2c

does not req Rad50 phos site S1202

  *** rRad50[Ab] boundby[WB] dsDNA[biotin] is detectable
    *** cells: none
    *** comment: reaction also contains rMre11 + rNbs1 + xAtm{dimer}[tAb]IP
    *** unaffected by: rRad50(S1202R) [substitution]
    *** source: 15790808-Fig-4b

no extras

  *** rRad50[Ab] boundby[WB] dsDNA[biotin] is detectable
    *** cells: none
    *** comment: reaction also contains rMre11 + rNbs1 + xAtm{dimer}[tAb]IP
    *** source: 15790808-Fig-2e



