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for correlation between Kinase Activity and phosphorylation see
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~/evidence/Egf-Evidence/Rsk3.irt.Egf.663.txt

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Basal Kinase activity
=====================

requires Nterm kinase domain

  *** xRsk3[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: COS7<xRsk3> in BMLS
    *** inhibited by: xRsk3(1-360) [substitution]
    *** inhibited by: xRsk3(1-373) [substitution]
    *** inhibited by: xRsk3(1-380) [substitution]
    *** enhanced by: xRsk3(1-389) [substitution]
    *** enhanced by: xRsk3(1-417) [substitution]
    *** unaffected by: xRsk3(1-422) [substitution]
    *** source: 10856237-Fig-2

act of Nterm kinase reqs phos(S386)

  *** xRsk3(1-389)[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: COS7<xRsk3(1-389)> in BMLS
    *** enhanced by: xRsk3(1-389/T365E) [substitution]
    *** enhanced by: xRsk3(1-389/S369E) [substitution]
    *** enhanced by: xRsk3(1-389/T365E/S369E) [substitution]
    *** source: 10856237-Fig-11a
  *** xRsk3(1-389)[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: COS7<xRsk3(1-389)> in BMLS
    *** inhibited by: xRsk3(1-389/S386A) [substitution]
    *** source: 10856237-Fig-3a
  *** xRsk3(1-389)[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: COS7<xRsk3(1-389)> in BMLS
    *** unaffected by: Egf (20 min) [stim]
    *** source: 10856237-Fig-3a

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Activation by overexpression of Pdpk1
=====================================

reqs phos(S227)

  *** xRsk3[tAb]IP IVKA(Rps6)[32P-ATP] is increased itpo xPdpk1
    *** cells: COS7<xRsk3> in BMLS
    *** inhibited by: xRsk3(S227E) [substitution]
    *** source: 10480933-Fig-8a
  *** xRsk3(1-360)[tAb]IP IVKA(Rps6)[32P-ATP] is increased itpo xPdpk1
    *** cells: COS7<xRsk3(1-360)> in BMLS
    *** inhibited by: xRsk3(1-360/S227E) [substitution]
    *** source: 10480933-Fig-3

partially reqs phos(S386)

  *** xRsk3[tAb]IP IVKA(Rps6)[32P-ATP] is increased itpo xPdpk1
    *** cells: COS7<xRsk3> in BMLS
    *** partially inhibited by: xRsk3(S386A) [substitution]
    *** source: 10480933-Fig-8a

does not req phos(S369) or phos(T577)

  *** xRsk3[tAb]IP IVKA(Rps6)[32P-ATP] is increased itpo xPdpk1
    *** cells: COS7<xRsk3> in BMLS
    *** unaffected by: xRsk3(S369A) [substitution]
    *** unaffected by: xRsk3(S369A/T577A) [substitution]
    *** source: 10480933-Fig-9a

==========================================================
Activation by rPdpk1 which phosphorylates Rsk3 on S227 
can be enhanced by rErk2 which phosphorylates Rsk3 on S386
==========================================================

  *** rRsk3[GST]IP IVKA(Rps6)[32P-ATP] is increased by rPdpk1(51-556)
    *** cells: none
    *** enhanced by: rErk2{act} [addition]
    *** source: 10480933-Fig-10a

===================================
Activation of Nterm kinase by Pdpk1
===================================

  *** xRsk3(1-373)[tAb]IP IVKA(S6peptide)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xRsk3(1-373)> in BMS
    *** enhanced by: pHMRSK [addition]
    *** inhibited by: HMRSK [addition]
    *** source: 12374740-Fig-6a
  *** xRsk3(1-373)[tAb]IP IVKA(S6peptide)[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xRsk3(1-373)> in BMS
    *** unaffected by: rRsk3(1-373/R119A) [substitution]
    *** source: 12374740-Fig-6a
  *** xRsk3(1-373)[tAb]IP phos[32P-ATP] is increased by rPdpk1
    *** cells: none
    *** IPfrom: HEK293<xRsk3(1-373)> in BMS
    *** unaffected by: rRsk3(1-373/R119A) [substitution]
    *** source: 12374740-Fig-6a
