===================
dephos of Smad2(T8)
===================

  *** xSmad2[tAb]IP phos(T8)[phosAb] is decreased by rCtdsp1
    *** cells: none
    *** IPfrom: HEK293T<xSmad3> in BMS
    *** inhibited by: rCtdsp1(D96E/D98N)"CIA" [substitution]
    *** source: 17035229-Fig-4b
  ------------------------------------------------------------------------
  *** xSmad2[tAb]IP phos(T8)[phosAb] is decreased by xCtdsp1[tAb]IP
    *** cells: none
    *** IP1from: HEK293T<xSmad3> in BMS
    *** IP2from: HEK293T in BMS
    *** inhibited by: xCtdsp1(D96E/D98N)"CIA"[tAb]IP [substitution]
    *** source: 17035229-Fig-4a
  *** xSmad2[tAb]IP phos(T8)[phosAb] is decreased by xCtdsp1[tAb]IP
    *** cells: none
    *** IP1from: HEK293T<xSmad3> in BMS
    *** IP2from: HEK293T in BMS
    *** unaffected by: Egf (90 min) [stim]
    *** unaffected by: Tgfb1 (90 min) [stim]
    *** inhibited by: xCtdsp1(D96E/D98N)"CIA"[tAb]IP [substitution]
    *** source: 17035229-Fig-7c
  ------------------------------------------------------------------------
  *** xSmad2[tAb]IP phos(T8)[phosAb] is decreased itpo xCtdsp1
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: MG132 [addition]
    *** source: 17035229-Fig-2d
  *** xSmad2[tAb]IP phos(T8)[phosAb] is decreased itpo xCtdsp1
    *** cells: HACAT in BMS
    *** source: 17035229-Fig-7d
  ------------------------------------------------------------------------
  *** Smad2[Ab]IP phos(T8)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** partially inhibited by: xCtdsp1 [addition]
    *** unaffected by: xCtdsp1(D96E/D98N)"CIA" [addition]
    *** source: 17035229-Fig-5c
  *** xSmad2[tAb]IP phos(T8)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xCtdsp1 [addition]
    *** source: 17035229-Fig-5b
  *** xSmad2[tAb]IP phos(T8)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xCtdsp1 [addition]
    *** unaffected by: xCtdsp1(D96E/D98N)"CIA" [addition]
    *** source: 17035229-Fig-5a
  *** xSmad2[tAb]IP phos(T8)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xCtdsp1 [addition]
    *** source: 17035229-Fig-2a
  *** xSmad2[tAb]IP phos(T8)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xCtdsp1 [addition]
    *** source: 17035229-Fig-1c

===============================
dephos of Smad2(S245/S250/S255)
===============================

  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is decreased by rCtdsp1
    *** cells: none
    *** IPfrom: HEK293 in BMS "treated with Egf for 30 min"
    *** source: 17085434-Fig-4b
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is decreased by rCtdsp1
    *** cells: none
    *** IPfrom: HEK293T<xSmad3> in BMS
    *** inhibited by: rCtdsp1(D96E/D98N)"CIA" [substitution]
    *** source: 17035229-Fig-4b
  ------------------------------------------------------------------------
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is decreased by xCtdsp1[tAb]IP
    *** cells: none
    *** IP1from: HEK293T<xSmad3> in BMS
    *** IP2from: HEK293T in BMS
    *** enhanced by: Egf (90 min) [stim]
    *** unaffected by: Tgfb1 (90 min) [stim]
    *** inhibited by: xCtdsp1(D96E/D98N)"CIA"[tAb]IP [substitution]
    *** source: 17035229-Fig-7c
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is decreased by xCtdsp1[tAb]IP
    *** cells: none
    *** IP1from: HEK293T<xSmad3> in BMS
    *** IP2from: HEK293T in BMS
    *** inhibited by: xCtdsp1(D96E/D98N)"CIA"[tAb]IP [substitution]
    *** source: 17035229-Fig-4a
  ------------------------------------------------------------------------
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is decreased itpo xCtdsp1
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xCtdsp1(D96E/D98N)"CIA" [substitution]
    *** source: 17035229-Fig-2b
  *** xSmad2[tAb]IP phos(T8)[phosAb] is decreased itpo xCtdsp1
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xCtdsp1(D96E/D98N)"CIA" [substitution]
    *** source: 17035229-Fig-2b
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is decreased itpo xCtdsp1
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: MG132 [addition]
    *** source: 17035229-Fig-2d
  *** xSmad2[phosAb] phos(S245/S250/S255)[phosAb] is decreased itpo xCtdsp1
    *** cells: HEK293 in BMS
    *** inhibited by: xCtdsp1(D97E)"CIA" [substitution]
    *** source: 17085434-Fig-2c
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is decreased itpo xCtdsp1
    *** cells: HACAT in BMS
    *** source: 17035229-Fig-7d
  ------------------------------------------------------------------------
  *** xSmad2[phosAb] phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293 in BMS "treated with Tgfb1 for 1 hr"
    *** inhibited by: xCtdsp1 [addition]
    *** source: 17085434-Fig-2c
  *** Smad2[Ab]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** partially inhibited by: xCtdsp1 [addition]
    *** unaffected by: xCtdsp1(D96E/D98N)"CIA" [addition]
    *** source: 17035229-Fig-5c
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xCtdsp1 [addition]
    *** source: 17035229-Fig-5b
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xCtdsp1 [addition]
    *** unaffected by: xCtdsp1(D96E/D98N)"CIA" [addition]
    *** source: 17035229-Fig-5a
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xCtdsp1 [addition]
    *** source: 17035229-Fig-2a
  *** xSmad2[tAb]IP phos(S245/S250/S255)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** inhibited by: xCtdsp1 [addition]
    *** source: 17035229-Fig-1c

========================
no dephos of Smad2(T220)
========================

  *** xSmad2[tAb]IP phos(T220)[phosAb] is unchanged by rCtdsp1
    *** cells: none
    *** IPfrom: HEK293T<xSmad3> in BMS
    *** unaffected by: rCtdsp1(D96E/D98N)"CIA" [substitution]
    *** source: 17035229-Fig-4b
  --------------------------------------------------------------
  *** xSmad2[tAb]IP phos(T220)[phosAb] is unchanged by xCtdsp1[tAb]IP
    *** cells: none
    *** IP1from: HEK293T<xSmad3> in BMS
    *** IP2from: HEK293T in BMS
    *** unaffected by: Egf (90 min) [stim]
    *** unaffected by: Tgfb1 (90 min) [stim]
    *** unaffected by: xCtdsp1(D96E/D98N)"CIA"[tAb]IP [substitution]
    *** source: 17035229-Fig-7c
  *** xSmad2[tAb]IP phos(T220)[phosAb] is unchanged by xCtdsp1[tAb]IP
    *** cells: none
    *** IP1from: HEK293T<xSmad3> in BMS
    *** IP2from: HEK293T in BMS
    *** unaffected by: xCtdsp1(D96E/D98N)"CIA"[tAb]IP [substitution]
    *** source: 17035229-Fig-4a
  --------------------------------------------------------------
  *** xSmad2[tAb]IP phos(T220)[phosAb] is unchanged itpo xCtdsp1
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdsp1(D96E/D98N)"CIA" [substitution]
    *** source: 17035229-Fig-2b
  --------------------------------------------------------------
  *** xSmad2[tAb]IP phos(T220)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdsp1 [addition]
    *** source: 17035229-Fig-2a
  *** xSmad2[tAb]IP phos(T220)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdsp1 [addition]
    *** source: 17035229-Fig-5b
  *** xSmad2[tAb]IP phos(T220)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** unaffected by: xCtdsp1 [addition]
    *** source: 17035229-Fig-1c

=============================
no dephos of Smad2(S463/S425)
=============================

  *** xSmad2[tAb]IP phos(S465/S467)[phosAb] is unchanged itpo xCtdsp1
    *** cells: HACAT in BMS
    *** source: 17035229-Fig-7d
  *** xSmad2[phosAb] phos(S465/S467)[phosAb] is detectable
    *** cells: HEK293 in BMS "treated with Tgfb1 for 1 hr"
    *** unaffected by: xCtdsp1 [addition]
    *** source: 17085434-Fig-2c
  *** xSmad2[tAb]IP phos(S465/S467)[phosAb] is detectable
    *** cells: HEK293T<xSmad3> in BMS
    *** partially inhibited by: xCtdsp1 [addition]
    *** source: 17035229-Fig-2a

=============================
coppt of endogenous proteins
=============================

  *** Smad2[Ab] copptby[WB] Ctdsp1[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 17035229-Fig-3c
  *** Smad2[Ab] snaggedby[WB] rCtdsp1[GST] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: rCtdsp1(D96E/D98N)"CIA" [substitution]
    *** source: 17035229-Fig-3a
