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Coprecipitation with other proteins in IL1R1C
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coppt with Tak1

  *** Tak1[Ab] copptby[WB] xNemo[GST] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 2+ 5+ 10+ 30 min
    *** inhibited by: xNemo(D311N)"unable-to-bind-to-K63-pUbiq chains" [substitution]
    *** source: 18180283-Fig-1a

  ubiq sites

  *** Ikk2[Ab] copptby[WB] xTak1[His] is increased irt IL1b (times)
    *** cells: HEK293T<xTak1> in BMS
    *** times: 0 5+ 10++ 15++ min
    *** inhibited by: xTak1(K158R) [substitution]
    *** source: 20038579-Fig-S3b

coppt with Traf6

  *** Traf6[Ab] copptby[WB] Ikk1[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMS
    *** times: 0 3+ 10 30 min
    *** source: 15836773-Fig-5b

  *** Traf6[Ab] copptby[WB] xNemo[GST] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 2+ 5+ 10+ 30 min
    *** inhibited by: xNemo(D311N)"unable-to-bind-to-K63-pUbiq chains" [substitution]
    *** source: 18180283-Fig-1a

  --- Traf6 does not bind Nemo directly

  *** Traf6[Ab] copptby[WB] Nemo[Ab] is unchanged irt IL1b (5 min)
    *** cells: HEK293<xIL1R1><xTLR4> in BMS
    *** comment: lysates were heated in SDS to dissociate Traf6-Irak1
    *** source: 18347055-Fig-1c
  *** Traf6[Ab] snaggedby[WB] rNemo[GST] is unchanged irt IL1b (5 min)
    *** cells: HEK293<xIL1R1><xTLR4> in BMS
    *** comment: lysates were heated in SDS to dissociate Traf6-Irak1
    *** source: 18347055-Fig-1c

coppt with Irak1

  *** Ikk1[Ab] copptby[WB] Irak1[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 5+ 10+ min
    *** source: 18180283-Fig-1c
  *** Ikk2[Ab] copptby[WB] Irak1[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 5+ 10+ min
    *** source: 18180283-Fig-1c
  *** Irak1[Ab] snaggedby[WB] rNemo[GST] is increased irt IL1b (5 min)
    *** cells: HEK293<xIL1R1><xTLR4> in BMS
    *** source: 18347055-Fig-1b
  *** Irak1[Ab] copptby[WB] Nemo[Ab] is increased irt IL1b (5 min)
    *** cells: HEK293<xIL1R1><xTLR4> in BMS
    *** source: 18347055-Fig-1c
  *** Irak1[Ab] copptby[WB] Nemo[Ab] is increased irt Lps (times)
    *** cells: HEK293<xIL1R1><xTLR4> in BMS
    *** times: 0 10+ 20+ 40+ 80+ min
    *** source: 18347055-Fig-1c
  *** Nemo[Ab] copptby[WB] Irak1[Ab] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 2+ 5+ 10+ 20++ 30++ 40++ 50++ 60++ min
    *** source: 18180283-Fig-1b

  --- requires Traf6 E3 activity

  *** Irak1[Ab] snaggedby[WB] rNemo[GST] is increased irt IL1b (5 min)
    *** cells: mEFs in BMS
    *** reqs: Traf6 [KO]
    *** source: 18347055-Fig-3b
  *** Irak1[Ab] snaggedby[WB] rNemo[GST] is increased irt IL1b (5 min)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** reqs: xTraf6 [omission]
    *** inhibited by: xTraf6(C70A)"E3-mut" [substitution]
    *** source: 18347055-Fig-3e
  *** Irak1[Ab] copptby[WB] Nemo[Ab] is increased irt IL1b (5 min)
    *** cells: mEFs in BMS
    *** reqs: Traf6 [KO]
    *** source: 18347055-Fig-3b

  --- requires Irak1 K63-Ubiq-attachment-site

  *** xIrak1[Ab] snaggedby[WB] rNemo[GST] is increased irt IL1b (5 min)
    *** cells: mEFs<Irak1~null> in BMS
    *** unaffected by: xIrak1(K134R)"Ubiq-binding-site-mut" [substitution]
    *** unaffected by: xIrak1(K180R)"Ubiq-binding-site-mut" [substitution]
    *** inhibited by: xIrak1(K134R/K180R)"Ubiq-binding-site-mut" [substitution]
    *** source: 18347055-Fig-4b

  --- requires Nemo K63-Ubiq-binding-site

  *** Irak1[Ab] snaggedby[WB] rNemo[GST] is increased irt IL1b (5 min)
    *** cells: HEK293<xIL1R1><xTLR4> in BMS
    *** inhibited by: rNemo(L329P)"impaired K63-Ubiq-binding mut" [substitution]
    *** source: 18347055-Fig-2a
  *** Irak1[Ab] copptby[WB] xNemo[GST] is increased irt IL1b (times)
    *** cells: HEK293<xIL1R1> in BMLS
    *** times: 0 2 5+ 10++ 30+ min
    *** comment: only Ubiq-Irak1[MW] is coppt
    *** inhibited by: xNemo(D311N)"unable-to-bind-to-K63-pUbiq chains" [substitution]
    *** source: 18180283-Fig-1a
  *** Irak1[Ab] copptby[WB] xNemo[Ab] is increased irt IL1b (5 min)
    *** cells: mEFs<Nemo~null><xNemo> in BMS
    *** inhibited by: xNemo(L329P)"impaired-K63-Ubiq-binding-mut" [substitution]
    *** source: 18347055-Fig-2b


  --- requires Ubc13--

  *** Irak1[Ab] copptby[WB] Nemo[Ab] is increased irt IL1b (times)
    *** cells: U2OS in BMS
    *** times: 0 10+ 20+ min
    *** reqs: Ubc13 [RNAi]
    *** comment: coppt Irak1 is ubiquitinated [WBMS]
    *** source: 19854138-Fig-5e

  not Ube2ds--

  *** Irak1[Ab] copptby[WB] Nemo[Ab] is increased irt IL1b (times)
    *** cells: U2OS in BMS
    *** times: 0 10+ 20+ min
    *** does not req: Ube2ds [RNAi]
    *** comment: coppt Irak1 is ubiquitinated [WBMS]
    *** source: 19854138-Fig-5b
  *** Irak1[Ab] copptby[WB] Nemo[Ab] is increased irt IL1b (times)
    *** cells: HEK293T in BMS
    *** times: 0 5 10+ min
    *** does not req: Ube2ds [RNAi]
    *** source: 19854138-Fig-S4d

  reqs K63 linked Ubiq--

  *** Irak1[Ab] copptby[WB] Nemo[Ab] is increased irt IL1b (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 10+ 15+ min
    *** reqs: Ubiq [RNAi]
    *** comment: coppt Irak1 is ubiquitinated [WBMS]
    *** source: 19854138-Fig-6b,6d
  *** Irak1[Ab] copptby[WB] Nemo[Ab] is unchanged irt IL1b (times)
    *** cells: U2OS<Ubiq~null><xUbiq(K63R)> in BMS
    *** times: 0 5+ 10+ min
    *** source: 19854138-Fig-6d













