Correlations between phos on S1981 and kinase activity:
 ~/evidence/IRad-Evidence/Atm.irt.IR.1449.txt

===============
Kinase Activity
===============

requires Tip60 activity and thus acetylation on K3016?

  *** Atm[Ab]IP IVKA(Tp53)[32P-ATP] is detectable
    *** cells: HELA in BMS
    *** inhibited by: xTip60(Q377E/G380E)"CIA" [addition]
    *** source: 16141325-Fig-1b
  *** Atm[Ab]IP IVKA(Tp53)[32P-ATP] is detectable
    *** cells: HELA<xTip60> in BMS
    *** inhibited by: xTip60(Q377E/G380E)"CIA" [substitution]
    *** source: 16141325-Fig-1b

requires Atm D2870 and N2875

  *** xAtm[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293T in BMS
    *** inhibited by: xAtm(D2870A/N2875K)"KD" [substitution]
    *** source: 9733515-Fig-1a
  *** xAtm[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293T<xAtm> in BMS
    *** inhibited by: xAtm(D2870A) [substitution]
    *** source: 10839544-Fig-2f

does not req phos on S1981

  *** xAtm[tAb]IP IVKA(Tp53)[32P-ATP] is detectable
    *** cells: GM9607<Atm~fmut><xAtm> in BMS
    *** inhibited by: xAtm(D2870A/N2875K)"KD" [substitution]
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 12556884-Fig-3a
  *** xAtm[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: HEK293T<xAtm> in BMS
    *** inhibited by: xAtm(D2870A/N2875K)"KD" [substitution]
    *** source: 12556884-Fig-1c
  *** xAtm[tAb]IP IVKA(peptide)[32P-ATP] is detectable
    *** cells: HEK293T<xAtm> in BMS
    *** inhibited by: xAtm(D2870A/N2875K)"KD" [substitution]
    *** source: 12556884-Fig-2e

===========================
Decrease in oligomerization
===========================

requires Mre11--

  *** xAtm{dimer}[tAb]IP oligomerization[2TagPpt] is decreased by (rRad50 + rMre11 + rNbs1)
    *** cells: none
    *** IPfrom: HEK293T<xAtm{dimer}> in BMS
    *** reqs: rMre11 [omission]
    *** source: 15790808-Fig-S2c

not Rad50--

  *** xAtm{dimer}[tAb]IP oligomerization[2TagPpt] is decreased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: none
    *** IPfrom: HEK293T<xAtm{dimer}> in BMS
    *** unaffected by: rRad50(S1202R) [substitution]
    *** source: 15790808-Fig-S2c

not dsDNA--

  *** xAtm{dimer}[tAb]IP oligomerization[2TagPpt] is decreased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: none
    *** IPfrom: HEK293T<xAtm{dimer}> in BMS
    *** does not req: dsDNA [omission]
    *** source: 15790808-Fig-3c

not phos on S1981

  *** xAtm{dimer}[tAb]IP oligomerization[2TagPpt] is decreased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: none
    *** IPfrom: HEK293T<xAtm{dimer}> in BMS
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 15790808-Fig-3d

============================
Kinase Activity of ATM dimer
============================

does not require dsDNA for autophos

  *** xAtm{dimer}[tAb]IP IVKA(auto)(S1981)[32P-ATP] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** does not req: dsDNA [omission]
    *** source: 15790808-Fig-3a

reqs dsDNA for phos of non-auto substrates

  *** xAtm{dimer}[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** comment: dsDNA must be linear - cut with DraIII BamHi EcoRV OK
    *** source: 15790808-Fig-2a
  *** xAtm{dimer}[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** comment: phos increases with dsDNA length but not concentration
    *** source: 15790808-Fig-2b
  *** xAtm{dimer}[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** reqs: dsDNA [omission]
    *** source: 15790808-Fig-1e
  *** xAtm{dimer}[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** reqs: dsDNA [omission]
    *** source: 15790808-Fig-3b
  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** comment: dsDNA must be linear - cut with DraIII BamHi EcoRV OK
    *** source: 15790808-Fig-2a
  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** comment: inhibited by substitution of linear dsDNA with dsDNA with hairpin ends
    *** source: 15790808-Fig-4c
  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** comment: phos increases with dsDNA length but not concentration
    *** source: 15790808-Fig-2b
  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** comment: unaffected by substitution of dsDNA (paired ends) with dsDNA (unpaired ends)
    *** comment: rRad50(S1202R) can substitute for RRad50 only if dsDNA with unpaired ends is used
    *** source: 15790808-Fig-4d
  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** reqs: dsDNA [omission]
    *** source: 15790808-Fig-1d
  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** reqs: dsDNA [omission]
    *** source: 15790808-Fig-1f
  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** reqs: dsDNA [omission]
    *** source: 15790808-Fig-3a
  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** reqs: dsDNA [omission]
    *** source: 15790808-Fig-4a

does not req ATM phos site S1981

  *** xAtm{dimer}[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 15790808-Fig-3b
  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** unaffected by: xAtm(S1981A) [substitution]
    *** source: 15790808-Fig-3a

requires Rad50-- and/or Mre11-- and/or Nbs1--

  *** xAtm{dimer}[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** reqs: rRad50 and/or rMre11 and/or rNbs1 [omission]
    *** source: 15790808-Fig-1e
  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** reqs: rRad50 and/or rMre11 and/or rNbs1 [omission]
    *** source: 15790808-Fig-1d

Rad50-- phos site S1202

  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** inhibited by: rRad50(S1202R) [substitution]
    *** source: 15790808-Fig-4a

requires Nbs1--

  *** xAtm{dimer}[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** reqs: rNbs1 [omission]
    *** reqs: rRad50 and/or rMre11 and/or rNbs1 [omission]
    *** source: 15790808-Fig-1f

no extras

  *** xAtm{dimer}[tAb]IP IVKA(auto)(S1981)[32P-ATP] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm{dimer}> in BMS
    *** source: 15790808-Fig-3a

==============================
Kinase Activity of ATM monomer
==============================

requires Mre11--

  *** xAtm[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rMre11 + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** inhibited by: rMre11(N117S)"ATLD3/4" [substitution]
    *** source: 15064416-Fig-1c
  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rMre11 + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** inhibited by: rMre11(N117S)"ATLD3/4" [substitution]
    *** source: 15064416-Fig-2d
  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rMre11 + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** reqs: rMre11 [omission]
    *** source: 15064416-Fig-2c

requires Nbs1--

  *** xAtm[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rMre11 + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** inhibited by: rNbs1(S343A) [substitution]
    *** source: 15064416-Fig-1d
  *** xAtm[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rMre11 + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** partially reqs: rNbs1 [omission]
    *** source: 15064416-Fig-1c
  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rMre11 + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** reqs: rNbs1 [omission]
    *** source: 15064416-Fig-2d
  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rMre11 + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** reqs: rNbs1 [omission]
    *** source: 15064416-Fig-2c

requires rRad50-- and/or rMre11-- and/or rNbs1--

  *** xAtm[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rMre11 + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** reqs: rRad50 and/or rMre11 and/or rNbs1 [omission]
    *** source: 15064416-Fig-1a

does not req dsDNA--

  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm> in BMS
    *** does not req: dsDNA [omission]
    *** source: 15790808-Fig-1a

requires Atm kinase activity

  *** xAtm[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rMre11 + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** inhibited by: xAtm(mnr)"kd"[tAb]IP [addition]
    *** source: 15064416-Fig-1h

nothing to compare to

  *** xAtm[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rRad50 + rMre11 + rNbs1(mnr)"p70")
    *** cells: HEK293T<xAtm> in BMS
    *** source: 15064416-Fig-1g
  *** xAtm[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rRad50 + rMre11(mnr)"delC" + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** source: 15064416-Fig-1e
  *** xAtm[tAb]IP IVKA(Chek2)(T68)[phosAb] is increased by (rRad50 + rMre11(N117S)"ATLD3/4" + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** source: 15064416-Fig-1f
  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rMre11 + rRad50 + rNbs1(mnr)"p70")
    *** cells: HEK293T<xAtm> in BMS
    *** source: 15064416-Fig-2h
  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rMre11 + rRad50 + rNbs1(S343A))
    *** cells: HEK293T<xAtm> in BMS
    *** source: 15064416-Fig-2e
  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rMre11(mnr)"ATLD1/2" + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** source: 15064416-Fig-2f
  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rMre11(N117S)"ATLD3/4" + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** source: 15064416-Fig-2g

no extras

  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rMre11 + rRad50 + rNbs1)
    *** cells: HEK293T<xAtm> in BMS
    *** source: 15064416-Fig-2a

  *** xAtm[tAb]IP IVKA(Tp53)(S15)[phosAb] is increased by (rRad50 + rMre11 + rNbs1 + dsDNA)
    *** cells: HEK293T<xAtm> in BMS
    *** does not req: rNbs1 [omission]
    *** reqs: rRad50 and/or rMre11 and/or rNbs1 [omission]
    *** source: 15790808-Fig-1a








