======================================================
direct phosphorylation of Mek1(S218/S222) by Mekk1-act
======================================================

  *** rMek1[32P] phos[32P-ATP] is increased by rMekk1(CatDom)"CA"
    *** cells: none
    *** inhibited by: rMek1(S218A) [substitution]
    *** inhibited by: rMek1(S222A) [substitution]
    *** unaffected by: rMek1(S212A) [substitution]
    *** source: 7624324-Fig-3a
  *** rMek1[32P] phos[32P-ATP] is increased by rMekk1(CatDom)"CA"
    *** cells: none
    *** source: 7624324-Fig-2[t]

contradiction

  *** rMek1(265-301)[32P] phos[32P-ATP] is unchanged by rMekk1(mnr)"C"
    *** cells: none
    *** source: 9677429-Fig-4


=================
direct activation
=================

  *** rErk2 IVKA(MBP)[32P-ATP] is increased by (rMekk1(CatDom)"CA" + rMek1)
    *** cells: none
    *** reqs: rMekk1(CatDom)"CA" [omission]
    *** bkg inhibited by: rMek1(S218A) [substitution]
    *** bkg inhibited by: rMek1(S222A) [substitution]
    *** unaffected by: rMek1(S212A) [substitution]
    *** source: 7624324-Fig-3c
  *** rErk2(K52R)[32P] phos[32P-ATP] is increased by (rMekk1(CatDom)"CA" + rMek1)
    *** cells: none
    *** reqs: rMekk1(CatDom)"CA" [omission]
    *** source: 7624324-Fig-2
  *** rErk2[CB] phos[WBMS] is increased by (rMekk1(CatDom)"CA" + rMek1)
    *** cells: none
    *** reqs: rMekk1(CatDom)"CA" [omission]
    *** inhibited by: rMek1(S218A) [substitution]
    *** inhibited by: rMek1(S222A) [substitution]
    *** unaffected by: rMek1(S212A) [substitution]
    *** source: 7624324-Fig-3b


=====================================
Mek1 activity is increased itpo Mekk1
=====================================

  *** xMek1[tAb]IP IVKA(Erk1)[32P-ATP] is increased itpo xMekk1
    *** cells: NIH3T3 in BMLS
    *** source: 9006902-Fig-3b
  *** xMek1[tAb]IP IVKA(Erk?)[32P-ATP] is increased itpo xMekk1(CatDom)"CA"
    *** cells: COS1 in BMS
    *** source: 9808624-Fig-2a
  *** xMek2[tAb]IP IVKA(Erk?)[32P-ATP] is increased itpo xMekk1(CatDom)"CA"
    *** cells: COS1 in BMS
    *** source: 9808624-Fig-2a
  *** Mek1[Ab]IP IVKA(Erk2)[32P-ATP] is increased itpo xMekk1(CatDom)
    *** cells: HEK293 in BMS
    *** source: 7624324-Fig-5a
  *** xMek1[tAb]IP IVKA(Erk2,MBP)[32P-ATP] is increased itpo xMekk1(CatDom)
    *** cells: HEK293 in BMS
    *** source: 7624324-Fig-5b
  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased itpo xMekk1(CatDom)
    *** cells: HEK293 in BMS
    *** source: 7624324-Fig-4[tr]
  *** xMek1[tAb]IP IVKA(Erk2)[32P-ATP] is increased itpo xMekk1(CatDom)
    *** cells: HEK293 in BMS
    *** source: 7624324-Fig-5b

=================================================
Mek1 total phosphorylation is increased itpo Mekk1
=================================================

  *** xMek1[tAb]IP phos[32Pi] is increased itpo xMekk1(CatDom)
    *** cells: HEK293 in BMS
    *** source: 7624324-Fig-4[tl]

====================
physical interaction
====================

  *** Mek1[Ab] copptby[WB] Mekk1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** unaffected by: Nocodazole [addition]
    *** unaffected by: Sorbitol (30 min) [stim]
    *** source: 10969079-Fig-8a
  *** xMekk1[tAb] copptby[WB] Mek1[Ab] is detectable
    *** cells: HEK293 in BMS
    *** source: 10969079-Fig-7a

