=====================
Relocation to Nucleus
=====================

not Ins--

  *** Stat3[Ab] locatedin(nucleus)[IHC] is increased irt IL6 (10 min)
    *** cells: HEPG2 in BMLS "pretreated with LY294002"
    *** unaffected by: Ins [cotreatment]
    *** source: 12456685-Fig-5
  *** Stat3[Ab]IP infraction(nuclear)[WB] is unchanged irt IL6 (tnr)
    *** cells: HEPG2 in BMLS "pretreated with LY294002"
    *** unaffected by: Ins [cotreatment]
    *** source: 12456685-Fig-6c

Chemicals

  *** Stat3[Ab] infraction(nuclear)[WB] is increased irt IL6 (times)
    *** cells: HEPG2 in BMS
    *** times: 0 2+ 5+ 10+ 20+ min
    *** unaffected by: Rottlerin [pretreatment]
    *** source: 11335711-Fig-3c
  *** Stat3[Ab] locatedin(nucleus)[IHC] is increased irt IL6 (15 min)
    *** cells: HEPG2 in BMS
    *** unaffected by: Rottlerin [pretreatment]
    *** source: 11335711-Fig-3d

Phosphorylation state in nucleus

  *** Stat3[Ab] infraction(nuclear)[WB] is increased irt IL6 (tnr)
    *** cells: HEPG2 in BMS
    *** comment: nuclear Stat3 is phosed on Y705 [phosAb]
    *** source: 10446219-Fig-3b
  *** Stat3[Ab] infraction(nuclear)[WB] is increased irt IL6 (times)
    *** cells: HEPG2 in BMS
    *** times: 0 2+ 5+ 10+ 15+ 30+ 60+ min
    *** comment: Stat3 in nuc-fraction is phosed on Y705 [phosAb]
    *** comment: Stat3 is phosed on S727 after relocaton to nuc
    *** source: 11335711-Fig-3c

No extras

  *** xStat3[tAb] locatedin(nucleus)[IHC] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: NIH3T3<xPias3><xMitf> in BMS
    *** source: 15572665-Fig-5a
  *** xStat3[tAb] locatedin(nucleus)[IHC] is increased irt (IL6 + IL6Rsol) (30 min)
    *** cells: B16<xPias3><xMitf> in BMS
    *** source: 15572665-Fig-7a

Contradictions

  *** Stat3[Ab] locatedin(nucleus)[IHC] is detectable-but unchanged irt IL6 (30 min)
    *** cells: hUVECs in BMLS
    *** source: 15919823-Fig-1a
  *** Stat3[Ab] locatedin(nucleus)[IHC] is detectable-but unchanged irt IL6 (30 min)
    *** cells: HELA in BMLS
    *** source: 15919823-Fig-1a
  *** Stat3[Ab] locatedin(nucleus)[IHC] is detectable-but unchanged irt IL6 (30 min)
    *** cells: NIH3T3 in BMLS
    *** source: 15919823-Fig-1a
  *** Stat3[Ab] locatedin(nucleus)[IHC] is detectable-but unchanged irt IL6 (30 min)
    *** cells: HEP3B in BMLS
    *** source: 15919823-Fig-1a
  *** Stat3[Ab] locatedin(nucleus)[IHC] is detectable-but unchanged irt IL6 (30 min)
    *** cells: HEP3B in BMLS
    *** source: 15919823-Fig-7



=====================================
Coprecipitation with nuclear proteins
=====================================

  *** Stat3[Ab] copptby[WB] Pias3[Ab] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: B16 in BMS
    *** source: 15572665-Fig-7b
  *** Stat3[Ab] copptby[WB] Pias3[Ab] is increased irt (IL6 + IL6Rsol) (tnr)
    *** cells: mBMMastCs in BMS "IL3-deprived"
    *** source: 15572665-Fig-6b
  *** Stat3[Ab] copptby[WB] Pias3[Ab] is increased irt IL6 (10 min)
    *** cells: M1 in BMS
    *** source: 9388184-Fig-2a
  *** Stat3[Ab] copptby[WB] Pias3[Ab] is increased irt IL6 (15 min)
    *** cells: HEPG2 in BMS
    *** source: 9724754-Fig-4e
  *** Stat3[Ab] copptby[WB] Pias3[Ab] is unchanged irt IL6 (10 min)
    *** cells: M1 in BMS
    *** source: 9388184-Fig-2a
  *** xStat3[Ab] copptby[WB] xPias3[Ab] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: NIH3T3 in BMS
    *** source: 15572665-Fig-3a
  *** xStat3[Ab] copptby[WB] xPias3[Ab] is increased irt (IL6 + IL6Rsol) (15 min)
    *** cells: NIH3T3<xMitf> in BMS
    *** inhibited by: xMitf(S409A) [substitution]
    *** source: 15572665-Fig-3a

Contradiction

  *** Stat3[Ab] copptby[WB] Pias1[Ab] is unchanged irt IL6 (15 min)
    *** cells: HEPG2 in BMS
    *** source: 9724754-Fig-4d

====================
DNA binding activity
====================

  *** Stat3[Ab] oligo-binding[EMSA] is increased irt IL6 (15 min)
    *** cells: A431 in BMLS "nuclear-extracts"
    *** source: 8140422-Fig-3b
  *** Stat3[Ab] oligo-binding[EMSA] is increased irt IL6 (15 min)
    *** cells: HEPG2 in BMS "nuclear-extracts"
    *** source: 8621622-Fig-6
  *** Stat3[Ab] oligo-binding[EMSA] is increased irt IL6 (15 min)
    *** cells: HEPG2 in BMS "nuclear-extracts"
    *** source: 8621622-Fig-7
  *** Stat3[Ab] oligo-binding[EMSA] is increased irt IL6 (times)
    *** cells: HEP3B in BMS "nuclear-extracts"
    *** times: 0 15+ 60+ 240+ 720+ 1080+ min
    *** source: 8621622-Fig-7
  *** Stat3[Ab] oligo-binding[EMSA] is increased irt IL6 (10 min)
    *** cells: ANBL6 in BMS "IL6 deprived for 24 hr, nuclear-extract"
    *** inhibited by: 15dPGJ2 [pretreatment]
    *** inhibited by: Troglitazone [pretreatment]
    *** unaffected by: WY14643 [pretreatment]
    *** source: 14975242-Fig-4b
  *** Stat3[Ab] oligo-binding[EMSA] is increased irt IL6 (10 min)
    *** cells: KAS61 in BMS "IL6 deprived for 24 hr, nuclear-extract"
    *** inhibited by: 15dPGJ2 [pretreatment]
    *** inhibited by: Troglitazone [pretreatment]
    *** unaffected by: WY14643 [pretreatment]
    *** source: 14975242-Fig-4a

===================
Reporter Activation
===================

inhibited by Pias3--

  *** Stat3-reporter[Luc] is increased irt (IL6 + IL6Rsol) (6 hr) 
    *** cells: NIH3T3 in BMS
    *** inhibited by: xPias3 [addition]    
    *** source: 15572665-Fig-3b

Rac1--

  *** Stat3-reporter[Luc] is increased irt IL6 (24 hr)
    *** cells: HEPG2 in BMS
    *** inhibited by: xRac1(T17N) [addition]
    *** source: 11335711-Fig-5b
  *** Stat3-reporter[Luc] is increased irt IL6 (24 hr)
    *** cells: HEPG2 in BMS
    *** partially inhibited by: xRac1(T17N) [addition]
    *** partially inhibited by: xMekk?(mnr)"DN" [addition]
    *** source: 10727406-Fig-4c

Mkk4--

  *** Stat3-reporter[Luc] is increased irt IL6 (24 hr)
    *** cells: HEPG2 in BMS
    *** partially inhibited by: xMkk4(mnr)"DN" [addition]
    *** source: 10727406-Fig-3b
  *** Stat3-reporter[Luc] is increased irt IL6 (24 hr)
    *** cells: HEPG2 in BMS
    *** partially inhibited by: xMkk4(mnr)"DN" [addition]
    *** source: 11335711-Fig-5c

Pckd--

  *** Stat3-reporter[Luc] is increased irt IL6 (24 hr)
    *** cells: HEPG2 in BMS
    *** partially inhibited by: xPkcd(1-298)"DN" [addition]
    *** source: 11335711-Fig-3f

Stat3 phos(S727)

  *** Stat3-reporter[Luc] is increased irt IL6 (24 hr)
    *** cells: HEPG2 in BMS
    *** partially inhibited by: xStat3(S727A) [addition]
    *** source: 10727406-Fig-1b

Vavs--

  *** Stat3-reporter[Luc] is increased irt IL6 (24 hr)
    *** cells: HEPG2 in BMS
    *** partially inhibited by: xVav?(mnr)"DN" [addition]
    *** enhanced by: xVav?(mnr)"CA" [addition]
    *** source: 10727406-Fig-4b

chemicals

  *** Stat3-reporter[Luc] is increased irt IL6 (16 hr)
    *** cells: KAS61 in BMS "IL6 deprived for 24 hr"
    *** inhibited by: 15dPGJ2 [pretreatment]
    *** inhibited by: Troglitazone [pretreatment]
    *** unaffected by: Troglitazone + GW9662 [pretreatment]
    *** source: 14975242-Fig-4c
  *** Stat3-reporter[Luc] is increased irt IL6 (16 hr)
    *** cells: KAS61 in BMS "IL6 deprived for 24 hr"
    *** inhibited by: 15dPGJ2 [pretreatment]
    *** inhibited by: Troglitazone [pretreatment]
    *** unaffected by: WY14643 [pretreatment]
    *** source: 14975242-Fig-4d
  *** Stat3-reporter[Luc] is increased irt IL6 (16 hr)
    *** cells: KAS61<xPparg> in BMS "IL6 deprived for 24 hr"
    *** inhibited by: 15dPGJ2 [cotreatment]
    *** inhibited by: Troglitazone [cotreatment]
    *** partially inhibited by: Troglitazone + GW9662 [cotreatment]
    *** unaffected by: WY14643 [cotreatment]
    *** source: 14975242-Fig-6d
  *** Stat3-reporter[Luc] is increased irt IL6 (16 hr)
    *** cells: KAS61<xPparg><xNcor2> in BMS "IL6 deprived for 24 hr"
    *** inhibited by: 15dPGJ2 [cotreatment]
    *** inhibited by: Troglitazone [cotreatment]
    *** partially inhibited by: Troglitazone + GW9662 [cotreatment]
    *** unaffected by: WY14643 [cotreatment]
    *** source: 14975242-Fig-6d
  *** Stat3-reporter[Luc] is increased irt IL6 (16 hr)
    *** cells: OCIMY5<Pparg~null> in BMS "IL6 deprived for 24 hr"
    *** unaffected by: 15dPGJ2 [pretreatment]
    *** unaffected by: Troglitazone [pretreatment]
    *** source: 14975242-Fig-5a
  *** Stat3-reporter[Luc] is increased irt IL6 (16 hr)
    *** cells: OCIMY5<Pparg~null><xPparg(mnr)"DNAbindingmut"> in BMS "IL6 deprived for 24 hr"
    *** unaffected by: 15dPGJ2 [pretreatment]
    *** unaffected by: Troglitazone [pretreatment]
    *** source: 14975242-Fig-5a
  *** Stat3-reporter[Luc] is increased irt IL6 (16 hr)
    *** cells: OCIMY5<Pparg~null><xPparg> in BMS "IL6 deprived for 24 hr"
    *** inhibited by: 15dPGJ2 [pretreatment]
    *** partially inhibited by: Troglitazone [pretreatment]
    *** source: 14975242-Fig-5a
