================================
Expressed Mekk2 is kinase active
================================

  *** xMekk2[tAb]IP IVKA(auto)[32P-ATP] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xMekk2(S277A) [substitution]
    *** inhibited by: xMekk2(S522A/S524A) [substitution]
    *** source: 16362041(D)

=================================
Expressed Mekk2 is phosphorylated
=================================

  *** xMekk2[tAb] phos[WBMS-PPase] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xMekk2(K385M) [substitution]
    *** source: 15695508-Fig-2a

===========================================
Features required for Mekk2 oligomerization
===========================================

  *** xMekk2(1-341)[tAb] copptby[WB] xMekk2(342-619)[GST] is undetectable
    *** cells: COS1 in BMS
    *** source: 15695508-Fig-3b
  *** xMekk2(1-341)[tAb] copptby[WB] xMekk2[GST] is undetectable
    *** cells: COS1 in BMS
    *** source: 15695508-Fig-3a
  *** xMekk2(342-424)[tAb] copptby[WB] xMekk2(342-619)[GST] is detectable
    *** cells: COS1 in BMS
    *** source: 15695508-Fig-3b
  *** xMekk2(342-619)[tAb] copptby[WB] xMekk2(342-619)[GST] is detectable
    *** cells: COS1 in BMS
    *** source: 15695508-Fig-3b
  *** xMekk2(342-619)[tAb] copptby[WB] xMekk2[GST] is detectable
    *** cells: COS1 in BMS
    *** source: 15695508-Fig-3a
  *** xMekk2(342-619)[tAb] oligomerization[2TagPpt] is detectable
    *** cells: COS1 in BMS
    *** comment: unaffected by PPase treatment of lysates
    *** comment: unaffected by substitution of one xMekk2(342-619) with xMekk2((342-619)/K385M)
    *** source: 15695508-Fig-4a
  *** xMekk2(342-619)[tAb] oligomerization[2TagPpt] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xMekk2(342-424) [addition]
    *** source: 15695508-Fig-3c
  *** xMekk2(342-619)[tAb] phos[WBMS-PPase] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xMekk2((342-619)/K385M) [substitution]
    *** source: 15695508-Fig-2b
  *** xMekk2(342-619)[tAb]IP IVKA(Mkk7)(sitenr)[phosAb] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xMekk2((342-619)/K385M) [addition]
    *** source: 15695508-Fig-4b
  *** xMekk2(342-619)[tAb]IP IVKA(Mkk7)(sitenr)[phosAb] is detectable
    *** cells: COS1 in BMS
    *** inhibited by: xMekk2((342-619)/K385M) [substitution]
    *** source: 15695508-Fig-2b
  *** xMekk2[tAb] copptby[WB] xMekk2(342-619)[GST] is detectable
    *** cells: COS1 in BMS
    *** source: 15695508-Fig-3b

==========================
Coprecipitations and Snags
==========================

Cdc42--

  *** xMekk2(CatDom)[Ab] snaggedby[WB] rCdc42{GDP}[GST] is undetectable
    *** cells: COS in BMS
    *** source: 9305638-Fig-6a
  *** xMekk2(CatDom)[Ab] snaggedby[WB] rCdc42{GTPgS}[GST] is undetectable
    *** cells: COS in BMS
    *** source: 9305638-Fig-6a

Ikk2--

  *** xMekk2[tAb] copptby[WB] xIkk2[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMekk2(K395M)"KD" [substitution]
    *** source: 15572679-Fig-8a

Sin1--

  *** xMekk2[tAb] copptby[WB] xSin1[tAb] is detectable
    *** cells: COS1 in BMS
    *** source: 15988011-Fig-2a

Nemo--

  *** xMekk2[tAb] copptby[WB] xNemo[tAb] is undetectable
    *** cells: HEK293 in BMS
    *** unaffected by: xMekk2(K395M)"KD" [substitution]
    *** source: 15572679-Fig-8a

Rac1--

  *** xMekk2(CatDom)[Ab] snaggedby[WB] rRac1{GDP}[GST] is undetectable
    *** cells: COS in BMS
    *** source: 9305638-Fig-6a
  *** xMekk2(CatDom)[Ab] snaggedby[WB] rRac1{GTPgS}[GST] is undetectable
    *** cells: COS in BMS
    *** source: 9305638-Fig-6a

Rhoa--

  *** xMekk2(CatDom)[Ab] snaggedby[WB] rRhoa{GDP}[GST] is undetectable
    *** cells: COS in BMS
    *** source: 9305638-Fig-6a
  *** xMekk2(CatDom)[Ab] snaggedby[WB] rRhoa{GTPgS}[GST] is undetectable
    *** cells: COS in BMS
    *** source: 9305638-Fig-6a

Ripk1--

  *** xMekk2[tAb] copptby[WB] Ripk1[Ab] is undetectable
    *** cells: HEK293T in BMS
    *** source: 14585994-Fig-4a

