===========
Sumoylation
===========

  *** rStat1(697-711)[Ab] upshift[WBMS] is increased by (rSumo1 + rSae1 + rUba2 + rUbe2i)
    *** cells: none
    *** inhibited by: rUbe2i(C93S) [substitution]
    *** inhibited by: rStat1((697-711)/K703R) [substitution]
    *** inhibited by: rStat1(697-711){phos} [substitution]
    *** source: 16857984-Fig-2b
  *** rStat1[Ab] upshift[WBMS] is increased by (rSumo1 + rSae1 + rUba2 + rUbe2i)
    *** cells: none
    *** unaffected by: rPias1 [addition]
    *** unaffected by: rRanbp2 [addition]
    *** source: 16857984-Fig-2a
  *** Stat1[Ab]IP upshift[WBMS] is detectable
    *** cells: HEK293<xSumo1><xUbe2i> in BMS
    *** unaffected by: Ifng (30 min) [stim]
    *** source: 16857984-Fig-1b
  *** xStat1[Ab]IP upshift[WBMS] is detectable
    *** cells: HEK293T<xSumo1><xUbe2i> in BMS
    *** source: 16857984-Fig-3a
  *** xStat1[Ab]IP upshift[WBMS] is detectable
    *** cells: mEFs<Stat1~null><xSumo1><xUbe2i> in BMS
    *** inhibited by: xStat1(K703R) [substitution]
    *** inhibited by: xStat1(E705A) [substitution]
    *** source: 16857984-Fig-3a
  *** xStat1[tAb] upshift[WBMS] is detectable
    *** cells: COS7<xSumo1> in BMS
    *** enhanced by: xStat1(S727E) [substitution]
    *** enhanced by: xStat1(S727D) [substitution]
    *** unaffected by: xStat1(S727A) [substitution]
    *** source: 17897103-Fig-5a
  *** xStat1[tAb] upshift[WBMS] is detectable
    *** cells: COS7<xSumo1><xPias1> in BMS
    *** enhanced by: xStat1(S727D) [substitution]
    *** source: 17897103-Fig-5b

=============
Oligo-binding
=============

  *** xStat1[Ab] snaggedby[WB] oligo[biotin] is detectable
    *** cells: HEK293T in BMS
    *** oligo: GAS sequence-not-reported
    *** inhibited by: xStat1(K410Q/K413Q) [substitution]
    *** unaffected by: xStat1(K410R/K413R) [substitution]
    *** source: 19171783-Fig-S1h

==================
Protein Expression
==================

  *** Stat1[Ab] prot-exp[WB] is detectable
    *** cells: mEFs in BMS
    *** partially reqs: Irf1 [KO]
    *** source: 17293456-Fig-3g

================
Phosphorylation
================

  *** xStat1[phosAb] phos(S727)[phosAb] is detectable
    *** cells: COS1 in BMS
    *** source: 11278353-Fig-3b
  *** xStat1[phosAb] phos(Y701)[phosAb] is detectable
    *** cells: U3A<Stat1~null> in BMS "treated with Ifna for 1 hr"
    *** inhibited by: xStat1(K410Q) [substitution]
    *** inhibited by: xStat1(K413Q) [substitution]
    *** source: 19171783-Fig-2f
  *** xStat1[tAb]IP phos(Y701)[phosAb] is detectable
    *** cells: COS1 in BMS "treated with Egf (30 min)"
    *** inhibited by: xGrb2 [addition]
    *** source: 14498832-Fig-8

===============
Oligomerization
===============

  *** xStat1[tAb] oligomerization[2TagPpt] is detectable
    *** cells: HEK293T in BMS
    *** unaffected by: xStat1(K410R/K413R) [substitution]
    *** unaffected by: xStat1(K410Q/K413Q) [substitution]
    *** source: 19171783-Fig-3i
  *** xStat1[tAb] oligomerization[2TagPpt] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** unaffected by: xStat1(K410Q/K413Q) [substitution]
    *** source: 19171783-Fig-3k

=================
DNA-oligo binding
=================

  *** xStat1{phos}[phosAb] snaggedby[WB] oligo[biotin] is detectable
    *** cells: HEK293T in BMS
    *** oligo: GAS sequence-not-reported
    *** inhibited by: xStat1(K410Q/K413Q) [substitution]
    *** unaffected by: xStat1(K410R/K413R) [substitution]
    *** source: 19171783-Fig-S1h
  *** Stat1{phos}[phosAb] snaggedby[WB] oligo[biotin] is detectable
    *** cells: HEK293T<Ptpn2~null> in BMS "treated with Ifna (20 min)"
    *** oligo: GAS sequence-not-reported
    *** unaffected by: ValproicAcid [addition]
    *** unaffected by: TrichostatinA [addition]
    *** source: 19171783-Fig-6c
  *** xStat1{phos}[phosAb] snaggedby[WB] oligo[biotin] is detectable
    *** cells: U3A<Stat1~null> in BMS "treated with (Ifna (1 hr) + Vanadate (30 min))"
    *** oligo: GAS sequence-not-reported
    *** unaffected by: xStat1(K410Q) [substitution]
    *** unaffected by: xStat1(K413Q) [substitution]
    *** source: 19171783-Fig-5h
  *** xStat1{phos}[phosAb] snaggedby[WB] oligo[biotin] is detectable
    *** cells: U3A<Stat1~null><Ptpn2~null> in BMS "treated with Ifna (1 hr)"
    *** oligo: GAS sequence-not-reported
    *** unaffected by: xStat1(K410Q) [substitution]
    *** unaffected by: xStat1(K413Q) [substitution]
    *** source: 19171783-Fig-6f

===================================
Coprecipitations, Snags, and Colocs
===================================

Cbp--

  *** xStat1(K410R/K413R)[Ab] snaggedby[WB] rCbp(450-720)[GST] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** inhibited by: rCbp(1-450) [substitution]
    *** partially inhibited by: rCbp(1460-1890) [substitution]
    *** source: 19171783-Fig-1n
  *** xStat1[Ab] snaggedby[WB] rCbp(450-720)[GST] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** inhibited by: rCbp(1-450) [substitution]
    *** partially inhibited by: rCbp(1460-1890) [substitution]
    *** source: 19171783-Fig-1n

IfnaR1--

  *** xStat1[Ab] colocwith[IHC] IfnaR1[Ab] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** unaffected by: xStat1(K410Q/K413Q) [substitution]
    *** comment: colocalization is in the cytoplasm
    *** source: 19171783-Fig-1j

IfnaR2--

  *** xStat1[Ab] colocwith[IHC] IfnaR2[Ab] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** unaffected by: xStat1(K410Q/K413Q) [substitution]
    *** comment: colocalization is in the cytoplasm
    *** source: 19171783-Fig-1j
  *** xStat1[Ab] copptby[WB] IfnaR2[Ab] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** unaffected by: xStat1(K410Q/K413Q) [substitution]
    *** source: 19171783-Fig-1k

Kpna1--

  *** xStat1[Ab] snaggedby[WB] rKpna1[GST] is detectable
    *** cells: U3A<Stat1~null> in BMS "treated with Ifna for 1 hr"
    *** inhibited by: xStat1(K410Q) [substitution]
    *** inhibited by: xStat1(K413Q) [substitution]
    *** source: 19171783-Fig-2f

Pias1--

  *** xStat1[tAb] copptby[WB] xPias1[tAb] is detectable
    *** cells: COS7<xSumo1> in BMS
    *** enhanced by: xStat1(S727D) [substitution]
    *** source: 17897103-Fig-5b

Ptpn2--

  *** xStat1(K410Q)[Ab] copptby[WB] xPtpn2(C216S/D182A)[Ab] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** inhibited by: xStat1 [substitution]
    *** source: 19171783-Fig-7a
  *** xStat1(K410Q/K413Q)[Ab] copptby[WB] xPtpn2(C216S/D182A)[Ab] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** inhibited by: xStat1 [substitution]
    *** source: 19171783-Fig-7a
  *** xStat1(K413Q)[Ab] copptby[WB] xPtpn2(C216S/D182A)[Ab] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** inhibited by: xStat1 [substitution]
    *** source: 19171783-Fig-7a

Stat2--

  *** xStat1[Ab] colocwith[IHC] Stat2[Ab] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** unaffected by: xStat1(K410Q/K413Q) [substitution]
    *** comment: colocalization is in the cytoplasm
    *** source: 19171783-Fig-3l
  *** xStat1[Ab] colocwith[IHC] Tyk2[Ab] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** unaffected by: xStat1(K410Q/K413Q) [substitution]
    *** comment: colocalization is in the cytoplasm
    *** source: 19171783-Fig-1j

Fer--

  *** Stat1[Ab] copptby[WB] Fer[Ab] is undetectable
    *** cells: NIH3T3 in BMS
    *** source: 10878010-Fig-9

Hdac1--

  *** Stat1[Ab] copptby[WB] Hdac1[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 19171783-Fig-4g

Hdac2--

  *** Stat1[Ab] copptby[WB] Hdac2[Ab] is undetectable
    *** cells: HEK293T in BMS
    *** source: 19171783-Fig-4g

Hdac3--

  *** Stat1[Ab] copptby[WB] Hdac3[Ab] is detectable
    *** cells: HEK293T in BMS
    *** source: 19171783-Fig-4g

Hdac8--

  *** Stat1[Ab] copptby[WB] Hdac8[Ab] is undetectable
    *** cells: HEK293T in BMS
    *** source: 19171783-Fig-4g

Ripk1--

  *** Stat1[Ab] copptby[WB] Ripk1[Ab] is undetectable
    *** cells: HELA in BMS
    *** unaffected by: Tnf (30 min) [stim]
    *** source: 10848577-Fig-2a

Tradd--

  *** Stat1[Ab] copptby[WB] Tradd[Ab] is detectable
    *** cells: 2FTGH in BMS
    *** source: 10848577-Fig-3e
  *** xStat1[Ab] copptby[WB] Tradd[Ab] is detectable
    *** cells: U3A<Stat1~null> in BMS
    *** inhibited by: xStat1(Y701F) [substitution]
    *** source: 10848577-Fig-3e

Traf2--

  *** Stat1[Ab] copptby[WB] Traf2[Ab] is undetectable
    *** cells: HELA in BMS
    *** unaffected by: Tnf (30 min) [stim]
    *** source: 10848577-Fig-2a

Sumo1--

  *** Stat1[Ab] copptby[WB] xSumo1[His] is detectable
    *** cells: HEK293<xUbe2i> in BMS
    *** unaffected by: Ifng (30 min) [stim]
    *** comment: coppt Stat1 is not phosed on Y701 [phosAb]
    *** source: 16857984-Fig-1b


