========================================
Smad2 Smad4 coppt requires active TgfbR1
=========================================

  *** xSmad4[tAb] copptby[WB] xSmad2[tAb] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: HEPG2 in BMS
    *** inhibited by: xTgfbR1(WT) [substitution]
    *** source: 9215638-Fig-3a
  *** xSmad4[tAb] copptby[WB] xSmad2[tAb] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: HEPG2 in BMS
    *** inhibited by: xSmad7 [addition]
    *** source: 9215638(D)
  *** xSmad4[tAb] copptby[WB] xSmad2[tAb] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: NIH3T3 in BMS
    *** inhibited by: xCav1 [addition]
    *** source: 11102446(D)

=======================================
Smad2(phos(S250/S255) coppts with Smad4  but is it required?
=======================================

  *** Smad4[Ab] copptby[WB] Smad2{phos(S250/S255)}[phosAb] is increased irt Tgfb1 (40 min)
    *** cells: RGM1 in BMLS
    *** source: 15326485-Fig-5a
  *** Smad4[Ab] copptby[WB] Smad2{phos(S250/S255)}[phosAb] is increased irt Tgfb1 (40 min)
    *** cells: RGM1 in BMLS "treated with SP600125"
    *** source: 15326485-Fig-5b

=======================================
Smad2(phos(S465/S467) coppts with Smad4
=======================================

  *** rSmad4[tAb] boundby[WB] rSmad2(452-467)[GST] is detectable
    *** cells: none
    *** comment: binding was enhanced by substitution with rSmad2(452-467) phosed on S465 and S467
    *** source: 9346966-Fig-7
  *** rSmad4[tAb] boundby[WB] rSmad2(464-467)[GST] is detectable
    *** cells: none
    *** comment: binding was enhanced by substitution with rSmad2(464-467) phosed on S465 and S467
    *** source: 9346966-Fig-7
  *** Smad4[Ab] copptby[WB] Smad2{phos(S465/S467)}[phosAb] is increased irt Tgfb1 (40 min)
    *** cells: RGM1 in BMLS
    *** source: 15326485-Fig-5a
  *** Smad4[Ab] copptby[WB] Smad2{phos(S465/S467)}[phosAb] is increased irt Tgfb1 (40 min)
    *** cells: RGM1 in BMLS "treated with SP600125"
    *** source: 15326485-Fig-5b
  *** xSmad2[tAb]IP boundby[WB] rSmad4(mnr)"LMH2"[GST] is detectable
    *** cells: none
    *** enhanced by: xSmad2{phos(S465/S467)} [substitution]
    *** comment: experimental details were not available so these are a guess
    *** source: 12191473-Fig-7a

=================================================
Smad2 Smad4 coppt does not req Smad2 sumoylation
=================================================

  *** Smad2[Ab] copptby[WB] Smad4[Ab] is increased irt Tgfb1 (tnr)
    *** cells: RI14 in BMS
    *** unaffected by: xSumo1 [addition]
    *** source: 12621041-Fig-4a
  *** xSmad2[Ab] copptby[WB] xSmad4[Ab] is increased itpo xTgfbR1(T202D)"CA"
    *** cells: HEK293 in BMS
    *** unaffected by: xSmad4(K159R/K113R) [substitution]
    *** source: 12621041-Fig-4b

=================================================
Smad2 Smad4 coppt does not req protein synthesis
=================================================

  *** Smad4[Ab] copptby[WB] Smad2[Ab] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS "treated with CHX"
    *** source: 12191474-Fig-4

=======================================
Smad2 Smad4 coppt is located in nucleus
=======================================

  *** Smad4[Ab] copptby[WB] Smad2[Ab] is increased irt Tgfb1 (1 hr)
    *** cells: HACAT in BMS
    *** comment: Smad4 ppt is located in nuclear fraction
    *** does not req: Ppm1a [RNAi]
    *** source: 16751101-Fig-4d

==========================================================
Smad2 Smad4 coppt does not require Smad2 acetyl sites
==========================================================

  *** xSmad4[tAb] copptby[WB] xSmad2[tAb] is increased itpo xTgfbR1(mnr)"CA"
    *** cells: HEK293T in BMS
    *** unaffected by: xSmad2(K19R/K20R/K39R) [substitution]
    *** unaffected by: xEp300 [addition]
    *** source: 17478422-Fig-6b

========================================================
Smad2 Smad4 coppt requires Smad3 phos(S423/S245)
because it is inhibited by addition of phosphatase PPm1a
which has been shown to dephos Smad3 at those sites
========================================================

  *** Smad4[Ab] copptby[WB] Smad2[Ab] is increased itpo xTgfbR1
    *** cells: HACAT in BMS
    *** inhibited by: xPpm1a [addition]
    *** unaffected by: rPpm1a(D239M)"CIA" [substitution]
    *** source: 16751101-Fig-4f

============================================
Smad2 Smad4 coppt does not require Smad4 NES
============================================

  *** xSmad2[tAb] copptby[WB] xSmad4[tAb] is increased irt Tgfb1 (1 hr)
    *** cells: C2C12 in BMS
    *** unaffected by: xSmad4(L146A/L148A)"NES-mutant" [substitution]
    *** source: 11265759-Fig-3a
  *** xSmad2[tAb] copptby[WB] xSmad4[tAb] is increased itpo xTgfbR1(T304D)"CA"
    *** cells: NIH3T3 in BMS
    *** unaffected by: xSmad4(del(142-153)) [substitution]
    *** unaffected by: xSmad4(del(153-223)) [substitution]
    *** unaffected by: xSmad4(del(263-302)) [substitution]
    *** unaffected by: xSmad4(del(223-302)) [substitution]
    *** unaffected by: xSmad4(del(153-302)) [substitution]
    *** unaffected by: xSmad4(del(153-320)) [substitution]
    *** source: 11074002-Fig-3a

==================
Required proteins
==================

Zfyve16--

  *** Smad4[Ab] copptby[WB] Smad2[Ab] is increased irt Tgfb1 (1 hr)
    *** cells: HEP3B in BMS
    *** reqs: Zfyve16 [RNAi]
    *** source: 17272273-Fig-5a

=====
other
=====

  *** xSmad4[tAb] copptby[WB] xSmad2[tAb] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS in BMS
    *** source: 9311995-Fig-5b
  *** xSmad4[tAb] copptby[WB] xSmad2[tAb] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: HEPG2 in BMS
    *** inhibited by: xSmad7 [addition]
    *** source: 9215638-Fig-3a
  *** xSmad2[tAb] copptby[WB] xSmad4[tAb] is increased itpo xTgfbR1(mnr)"CA"
    *** cells: COS1 in BMLS
    *** unaffected by: xVps39 [addition]
    *** source: 12941698-Fig-8a
  *** xSmad2[tAb] copptby[WB] xSmad4[tAb] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS1 in BMS
    *** unaffected by: xTgfbRap1 [addition]
    *** source: 11278302(D)
  *** xSmad2[tAb] copptby[WB] xSmad4[tAb] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: HEK293 in BMS
    *** source: 11027280(D)
  *** Smad4[Ab] copptby[WB] Smad23s[Ab] is increased irt Tgfb1 (40 min)
    *** cells: RGM1 in BMLS
    *** source: 15326485-Fig-5a
  *** Smad4[Ab] copptby[WB] Smad23s[Ab] is increased irt Tgfb1 (40 min)
    *** cells: RGM1 in BMLS "treated with SP600125"
    *** source: 15326485-Fig-5b
  *** xSmad4[35S] copptby[WB] xSmad2[35S] is increased irt Tgfb1 (tnr)
    *** cells: COS<xTgfbR1><xTgfbR2> in BMS
    *** source: 9311995-Fig-6d
  *** Smad2[Ab] copptby[WB] Smad4[Ab] is increased irt Tgfb1 (1 hr)
    *** cells: HEPG2 in BMS
    *** source: 12543979-Fig-2
  *** Smad4[Ab] copptby[WB] Smad2[Ab] is increased irt Tgfb1 (1 hr)
    *** cells: HEPG2 in BMS
    *** source: 12543979-Fig-2
  *** xSmad4[tAb] copptby[WB] xSmad2[tAb] is increased itpo xTgfbR1(mnr)"CA"
    *** cells: bAECs in BMS
    *** source: 10085121-Fig-5b
  *** xSmad4[tAb] copptby[WB] xSmad2[tAb] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS1 in BMS
    *** inhibited by: xTgfbR1(WT) [substitution]
    *** source: 9865696-Fig-3c
  *** xSmad4[tAb] copptby[WB] xSmad2[tAb] is increased itpo xTgfbR1(T204D)"CA"
    *** cells: COS1 in BMS
    *** unaffected by: xSara [addition]
    *** source: 9865696-Fig-3c

