========================
Phosphorylation on S1981
========================
May require Tip60 and pre-acetylation but evidence is weak
see ~/evidence/DSB-Evidence/Atm.phos2.irt.IR.1449.txt
for other phos sites

not Atr--

  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 15+ 30+ 45+ min
    *** does not req: Atr [RNAi]
    *** source: 16431910-Fig-1c

not Brca1--

  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: HCC1937<Brca1~fmut><xBrca1> in BMS
    *** does not req: xBrca1 [omission]
    *** source: 15175241-Fig-2b
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Brca1 [RNAi]
    *** source: 19001859-Fig-1a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: hFibros in BMS
    *** times: 0 15+ 60+ min
    *** does not req: Brca1 [RNAi]
    *** source: 19001859-Fig-1b
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (tnr)
    *** cells: hFibros in BMS
    *** does not req: Brca1 [RNAi]
    *** source: 19001859-Fig-1d

not Cop1--

  *** Atm[Ab] phos(S1981)[phosAb] is increased irt IR (2 hr)
    *** cells: U2OS in BMS
    *** unaffected by: xCop1 [addition]
    *** unaffected by: xCop1(S387A) [addition]
    *** source: 16931761-Fig-1d

Kdm6b-- Kdm4a-- Kdm4d--  ???

  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (tnr)
    *** cells: HEK293T in BMS
    *** unaffected by: xKdm6b [addition]
    *** inhibited by: xKdm4a [addition]
    *** inhibited by: xKdm4d [addition]
    *** source: 19783983-Fig-4e

not Mdc1--

  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: U2OS in BMS
    *** times: 0 5++ 15++ 30++ 60++ 120++ 180+ 240+ 300+ 360+ 420+ 480+ min
    *** does not req: Mdc1 [RNAi]
    *** source: 15201865-Fig-S5
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (tnr)
    *** cells: mEFs in BMS
    *** does not req: Mdc1 [KO]
    *** source: 16427009-Fig-4b
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (tnr)
    *** cells: mEFs in BMS
    *** does not req: Mdc1 [KO]
    *** source: 16427009-Fig-4d
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 19001859-Fig-1a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: hFibros in BMS
    *** times: 0 15+ 60+ min
    *** does not req: Mdc1 [RNAi]
    *** source: 19001859-Fig-1b
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (tnr)
    *** cells: hFibros in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 19001859-Fig-1d
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Mdc1 [RNAi]
    *** source: 16377563-Fig-S5a
  --------------- contradiction ------------------------------------
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (10 min)
    *** cells: HELA in BMS
    *** partially reqs: Mdc1 [RNAi]
    *** source: 14695167-Fig-4b
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (10 min)
    *** cells: XPCEN12<Xpc~fmut> in BMS
    *** partially reqs: Mdc1 [RNAi]
    *** source: 14695167-Fig-4c

Mre11--  ???

  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: D6809<Mre11~fmut><xMre11> in BMS
    *** bkg reqs: xMre11 [omission]
    *** unaffected by: xMre11(NLS) [substitution]
    *** source: 16478990-Fig-4c
  ------------------ contradiction --------------------------------
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: hFibros<Mre11~fmut><xMre11> in BMS
    *** does not req: xMre11 [omission]
    *** unaffected by: xMre11(mnr)"CIA" [substitution]
    *** source: 16327781-Fig-4a
  ---------------- Mre11 fmutants alone ---------------------------
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (times)
    *** cells: ATLD6<Mre11~fmut> in BMS
    *** times: 0 15+ 60+ min
    *** comment: the increase is significantly less than that seen in C3ABR
    *** source: 16858402-Fig-4b


Nbs1--  ???

  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** does not req: xNbs1 [omission]
    *** comment: xNbs1 is req at lower (0.3 Gy) doses
    *** source: 15175241-Fig-2a
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** times: 0 15+ 30+ 60+ 120+ min
    *** does not req: xNbs1 [omission]
    *** comment: xNbs1 is partially req at lower (0.5 Gy) doses and earlier (15 min) times
    *** source: 15175241-Fig-2b
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: hFibros<Nbs1~fmut><xNbs1> in BMS
    *** does not req: xNbs1 [omission]
    *** unaffected by: xNbs1(S278A/S343A/S397A) [substitution]
    *** source: 16327781-Fig-2c
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (60 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** does not req: xNbs1 [omission]
    *** unaffected by: xNbs1(1-652) [substitution]
    *** enhanced by: xNbs1(401-754) [substitution]
    *** enhanced by: xNbs1(S278A/S343A) [substitution]
    *** source: 15234984-Fig-2b
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** partially reqs: xNbs1 [omission]
    *** inhibited by: xNbs1(1-652) [substitution]
    *** enhanced by: xNbs1(401-754) [substitution]
    *** enhanced by: xNbs1(S278A/S343A) [substitution]
    *** source: 15234984-Fig-2a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** times: 0 15+ 30+ 60+ min
    *** partially reqs: xNbs1 [omission]
    *** partially inhibited by: xNbs1(1-734)"delC" [substitution]
    *** source: 15758953-Fig-3a
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** partially reqs: xNbs1 [omission]
    *** inhibited by: xNbs1(1-652)"Nb652" [substitution]
    *** unaffected by: xNbs1(454-754)"NbFR5" [substitution]
    *** source: 16478990-Fig-3a
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** partially reqs: xNbs1 [omission]
    *** inhibited by: xNbs1(1-735)"dAtm" [substitution]
    *** unaffected by: xNbs1(454-754)"NbFR5" [substitution]
    *** inhibited by: xNbs1(454-735)"NbFR5sAtm" [substitution]
    *** source: 16478990-Fig-6b
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt DSB-Induction (16 hr)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** bkg reqs: xNbs1 [omission]
    *** source: 17486112-Fig-S3a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (10 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** partially reqs: xNbs1 [omission]
    *** unaffected by: xNbs1(S343A) [substitution]
    *** source: 14695167-Fig-3c
  --------------- Nbs1 fmutants alone ----------------------------
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (times)
    *** cells: NBS03LA<Nbs1~fmut> in BMS
    *** times: 0 15+ 60+ min
    *** comment: the increase is significantly less than that seen in C3ABR
    *** source: 16858402-Fig-4a

Rad50--  ???

  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: HCT116 in BMS
    *** times: 0 3++ 10++ 30++ 90+ min
    *** partially reqs: Rad50 [RNAi]
    *** source: 19783983-Fig-2b
  --------------- Rad50 fmutants alone ----------------------------
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (60 min)
    *** cells: HA239<Rad50~fmut> in BMS
    *** comment: the increase is significantly less than that seen in C3ABR
    *** source: 16858402-Fig-4c
  *** Atm[Ab]IP phos(S1891)[phosAb] is unchanged irt IR (times)
    *** cells: F239HTERT<Rad50~fmut> in BMS
    *** times: 0+ 15++ 30+ min
    *** source: 21149446-Fig-6c

not Rnf8--

  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** does not req: Rnf8 [RNAi]
    *** source: 18006705-Fig-S14

not Smc1a--

  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: mEFs in BMS
    *** unaffected by: Smc1a(S957A/S966A) [knockin]
    *** source: 15175241-Fig-5b

Suv39h1-- and/or Suv39h2--

  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: mEFs in BMS
    *** times: 0 1+ 2+ 4+ hr
    *** reqs: Suv39h1 and/or Suv39h2 [KO]
    *** source: 19783983-Fig-S7
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (tnr)
    *** cells: mEFs in BMS
    *** reqs: Suv39h1 and/or Suv39h2 [KO]
    *** source: 19783983-Fig-4b

Tip60--

  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: HELAS3<xTip60> in BMS
    *** times: 0 30+ 120+ 240+ min
    *** inhibited by: xTip60(Y47A)"CD" [substitution]
    *** source: 19783983-Fig-S2b
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (tnr)
    *** cells: HELAS3 in BMS
    *** unaffected by: xTip60 [addition]
    *** source: 19783983-Fig-1b
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (tnr)
    *** cells: HELAS3<xTip60> in BMS
    *** unaffected by: xTip60(W26A) [substitution]
    *** unaffected by: xTip60(F50A) [substitution]
    *** inhibited by: xTip60(F43A) [substitution]
    *** inhibited by: xTip60(Y47A)"CD" [substitution]
    *** source: 19783983-Fig-1b

Tp53bp1--

  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: U2OS in BMS
    *** reqs: Tp53bp1 [RNAi]
    *** source: 19001859-Fig-1a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: hFibros in BMS
    *** times: 0 15+ 60+ min
    *** partially reqs: Tp53bp1 [RNAi]
    *** source: 19001859-Fig-1b
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (tnr)
    *** cells: hFibros in BMS
    *** partially reqs: Tp53bp1 [RNAi]
    *** source: 19001859-Fig-1d
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (10 min)
    *** cells: NBS1LBI<Nbs1~fmut> in BMS
    *** partially reqs: Tp53bp1 [RNAi]
    *** source: 14695167-Fig-5a
  ---------------- contradiction ---------------------------------
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: NBS1LBI<Nbs1~fmut><xNbs1> in BMS
    *** does not req: Tp53bp1 [RNAi]
    *** source: 14695167-Fig-5b

not Trf1--

  *** xAtm[phosAb] phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: HEK293<xAtm> in BMS
    *** unaffected by: xTrf1 [addition]
    *** source: 15314656-Fig-3a

inhibited by Trf2--

  *** xAtm[phosAb] phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: IMR90<xAtm> in BMS
    *** partially inhibited by: xTrf2 [addition]
    *** source: 15314656-Fig-3a
  *** xAtm[phosAb] phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: HEK293<xAtm> in BMS
    *** inhibited by: xTrf2 [addition]
    *** source: 15314656-Fig-3a

requires Atm kinase activity

  *** xAtm[tAb]IP phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: HEK293T<xAtm> in BMS
    *** inhibited by: xAtm(D2870A/N2875K)"KD" [substitution]
    *** source: 12556884-Fig-2c
  *** xAtm[tAb]IP phos[32Pi] is increased irt IR (30 min)
    *** cells: HEK293T<xAtm> in BMS
    *** inhibited by: xAtm(D2870A/N2875K)"KD" [substitution]
    *** source: 12556884-Fig-1a
  ------------ AT1ABR contains a kinase dead mutant of Atm --------
  *** Atm[Ab]IP phos(S1891)[phosAb] is unchanged irt IR (1 hr)
    *** cells: AT1ABR<Atm~fmut> in BMS
    *** source: 16858402-Fig-3c

chemicals

  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (45 min)
    *** cells: RAJI in BMS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 16461339-Fig-1a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (45 min)
    *** cells: RAJI in BMS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 16461339-Fig-S1
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** partially inhibited by: KU55933 [pretreatment]
    *** source: 16858402-Fig-3e
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** partially inhibited by: Wortmannin [pretreatment]
    *** source: 16858402-Fig-3d
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: U2OS in BMS
    *** unaffected by: Roscovitine [pretreatment]
    *** source: 16327781-Fig-7a
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** inhibited by: KU55933 [pretreatment]
    *** source: 21149446-Fig-4a
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** partially inhibited by: KU55933 [pretreatment]
    *** source: 21149446-Fig-5a
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** unaffected by: Wortmannin [pretreatment]
    *** source: 21149446-Fig-4b
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt DSB-Induction (times)
    *** cells: MCF7 in BMS
    *** times: 0 4+ 8++ 12+ 16+ 20++ hr
    *** inhibited by: KU55933 [cotreatment]
    *** source: 17486112-Fig-2a
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: 1070SK in BMS
    *** inhibited by: Wortmannin [pretreatment]
    *** source: 12556884-Fig-2b

no extras

  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** source: 21149446-Fig-3a
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (times)
    *** cells: C3ABR in BMS
    *** times: 0 6+ 15+ 30+ 60+ 120+ 240+ 480+ 1440 min
    *** source: 21149446-Fig-3b
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (times)
    *** cells: C3ABR in BMS
    *** times: 0 6+ 15+ 30+ 60+ 120+ 240+ 480+ 1440+ min
    *** source: 21149446-Fig-2b
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (times)
    *** cells: C3ABR in BMS
    *** times: 0+ 15++ 30+ min
    *** source: 21149446-Fig-6c
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (times)
    *** cells: C3ABR in BMS
    *** times: 0+ 15++ 30++ min
    *** source: 21149446-Fig-6a
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (times)
    *** cells: C3ABR in BMS
    *** times: 0+ 15++ 30++ min
    *** source: 21149446-Fig-6b
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: GM637 in BMS
    *** source: 15234984-Fig-2a
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (60 min)
    *** cells: GM637 in BMS
    *** source: 15234984-Fig-2b
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: 1070SK in BMS
    *** comment: intensities increased at 0.1 Gy and 0.3 Gy
    *** source: 12556884-Fig-5c
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: 1070SK in BMS
    *** comment: equal intensities were seen for 1-9 Gy
    *** source: 12556884-Fig-5c
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: 1070SK in BMS
    *** times: 0 1+ 5+ hr
    *** source: 12556884-Fig-2d
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: 1070SK in BMS
    *** times: 0 5+ 10+ 15+ min
    *** comment: 2 Gy of IR was used
    *** source: 12556884-Fig-5b
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: 1070SK in BMS
    *** times: 0 15+ 30+ 60+ 120+ 180+ 240+ 300+ 360+ min
    *** comment: 2 Gy of IR was used
    *** source: 12556884-Fig-5a
  *** Atm[Ab]IP phos[32Pi] is increased irt IR (30 min)
    *** cells: GM536 in BMS
    *** source: 12556884-Fig-1b
  *** Atm[phosAb] phos(S1981)[IHC] is increased irt IR (times)
    *** cells: 1070SK in BMS
    *** times: 0 5++ 60+ min
    *** comment: 1 Gy IR was used
    *** comment: pattern is nuclear, bright, diffuse at 5 min
    *** comment: pattern is nuclear and speckled at 60 min
    *** source: 12556884-Fig-6a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: 1070SK in BMS
    *** source: 12556884-Fig-2b
  *** xAtm[tAb]IP phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: GM9607<Atm~fmut><xAtm> in BMS
    *** source: 12556884-Fig-2g
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: 1070SK in BMS
    *** source: 15175241-Fig-1a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: GM637 in BMS
    *** source: 15175241-Fig-S1a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (45 min)
    *** cells: GM12072 in BMS
    *** source: 16461339-Fig-1c
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (45 min)
    *** cells: GM18366<Atr~fmut> in BMS
    *** source: 16461339-Fig-1c
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 10+ 30+ 60+ 120++ 240+ 480+ 720 min
    *** source: 16474843-Fig-1c
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (15 min)
    *** cells: GM637 in BMS
    *** source: 16478990-Fig-4c
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (times)
    *** cells: C3ABR in BMS
    *** times: 0 15+ 60+ min
    *** source: 16858402-Fig-4a
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (times)
    *** cells: C3ABR in BMS
    *** times: 0 15+ 60+ min
    *** source: 16858402-Fig-4b
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (times)
    *** cells: C3ABR in BMS
    *** times: 0 15++ 30++ 60++ 120++ 240++ 480++ 1440+ min
    *** source: 16858402-Fig-3a
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** source: 16858402-Fig-3b
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** source: 16858402-Fig-3c
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (60 min)
    *** cells: C3ABR in BMS
    *** source: 16858402-Fig-4c
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: HEK293T in BMS
    *** source: 19001859-Fig-1e
  *** xAtm[phosAb] phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: HEK293T<xAtm> in BMS
    *** source: 19001859-Fig-1e
  *** xAtm[tAb]IP phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: AT22IJET<Atm~fmut><xAtm> in BMS
    *** source: 19001859-Fig-1e
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: U2OS in BMS
    *** times: 0 5+ 10+ 15+ 30+ 60+ min
    *** source: 16327781-Fig-5b
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: HELA in BMS
    *** source: 16141325-Fig-1a
  *** Atm[Ab]IP phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: HEK293T in BMS
    *** times: 0 20+ 40+ 120+ min
    *** source: 17923702-Fig-2c
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt DSB-Induction (8 hr)
    *** cells: MCF7 in BMS
    *** source: 17486112-Fig-1b
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt DSB-Induction (12 hr)
    *** cells: MCF7 in BMS "arrested in G1 by serum-depletion for 24 hr"
    *** source: 17486112-Fig-3a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt DSB-Induction (24 hr)
    *** cells: HEK293T in BMS
    *** times: 0 30 60+ 120+ 240+ 480+ 960++ 1440++ min
    *** source: 17486112-Fig-S1a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt DSB-Induction (times)
    *** cells: MCF7 in BMS
    *** times: 0 30 60+ 120+ 240+ 480+ 960++ 1440++ min
    *** source: 17486112-Fig-1a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: HEK293T in BMS
    *** source: 17486112-Fig-S1a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: MCF7 in BMS
    *** source: 17486112-Fig-1a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (tnr)
    *** cells: MCF7 in BMS
    *** source: 17486112-Fig-1b
  *** xAtm[phosAb] phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS
    *** times: 0 10+ 60+ min
    *** inhibited by: xAtm(S1981A) [substitution]
    *** source: 20026654-Fig-3
  *** xAtm[tAb]IP phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS
    *** inhibited by: xAtm(S1981A) [substitution]
    *** source: 20026654-Fig-4b
  *** xAtm[tAb]IP phos(S1981)[phosAb] is increased irt IR (1 hr)
    *** cells: AT5BIVA<Atm~fmut><xAtm> in BMS
    *** source: 20026654-Fig-4a
  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (times)
    *** cells: HELA in BMS
    *** times: 0 10+ 60+ min
    *** source: 14695167-Fig-2b

other information

  *** Atm[phosAb] phos(S1981)[phosAb] is increased irt IR (30 min)
    *** cells: T24 in BMS
    *** comment: cells were synchronized at G0 by density arrest
    *** comment: unaffected by time after replating (12-32 hr)
    *** source: 16327781-Fig-6e
  *** Atm[Ab]IP phos(S1891)[phosAb] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** comment: this site/CL/Ab responds to 20X less Gy than S367 and S1893
    *** source: 21149446-Fig-2a

reqs acetylation before phos

  *** xAtm[Ab]IP phos(S1981)[phosAb] is increased irt IR (40 min)
    *** cells: GM5849<Atm~fmut><xAtm> in BMS
    *** inhibited by: xAtm(K3016R) [substitution]
    *** source: 17923702-Fig-3b

===============
Kinase Activity
===============

  *** Atm[Ab]IP IVKA(Tp53)(S15)[phosAb] is increased irt IR (times)
    *** cells: GM536 in BMS
    *** times: 0+ 20++ 60++ min
    *** source: 9733515-Fig-3b
  *** Atm[Ab]IP IVKA(Tp53)(S15)[phosAb] is increased irt IR (times)
    *** cells: GM2184 in BMS
    *** times: 0 20++ 60++ min
    *** source: 9733515-Fig-3b

  *** Atm[Ab]IP IVKA(auto)[32P-ATP] is detectable-but unchanged irt IR (times)
    *** cells: GM536 in BMS
    *** times: 0+ 20+ 60+ min
    *** source: 9733515-Fig-3a
  *** Atm[Ab]IP IVKA(auto)[32P-ATP] is detectable-but unchanged irt IR (times)
    *** cells: GM2184 in BMS
    *** times: 0+ 20+ 60+ min
    *** source: 9733515-Fig-3a

  *** Atm[Ab]IP IVKA(peptide)[32P-ATP] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** comment: peptide is rAtm(1887-1898)
    *** source: 16858402-Fig-2c
  *** Atm[Ab]IP IVKA(peptide)[32P-ATP] is increased irt IR (1 hr)
    *** cells: C3ABR in BMS
    *** comment: peptide is rAtm(1887-1898)
    *** source: 16858402-Fig-2d
  *** Atm[Ab]IP IVKA(peptide)[32P-ATP] is unchanged irt IR (1 hr)
    *** cells: AT1ABR<Atm~fmut> in BMS
    *** comment: peptide is rAtm(1887-1898)
    *** source: 16858402-Fig-2d

