=================
Required Proteins
=================

Nemo--

  *** Mekk1[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: 70Z3 in BMS "P100 fraction"
    *** times: 0 10+ 30 min
    *** reqs: Nemo [KO]
    *** source: 18635759-Fig-4b

Traf2--

  *** Mekk1[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS "P100 fraction"
    *** times: 0 10+ 30 min
    *** reqs: Traf2 [RNAi]
    *** source: 18635759-Fig-4c

not Traf3--

  *** Mekk1[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS "P100-fraction"
    *** times: 0 5+ 10+ 30 min
    *** does not req: Traf3 [RNAi]
    *** source: 18635759-Fig-S12

Ubc13--

  *** Mekk1[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: A20 in BMS "P100 fraction"
    *** times: 0 10+ 30 min
    *** reqs: Ubc13 [RNAi]
    *** source: 18635759-Fig-4c

==============
Mekk1 features
==============

  *** Mekk1[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS "P100 fraction"
    *** times: 0 10+ 30 min
    *** inhibited by: kMekk1(1-1188)"del-kinase-domain" [addition]
    *** source: 18635759-Fig-4a
  *** Mekk1[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS "P100-fraction"
    *** times: 0 10+ 30 min
    *** comment: coppt Mekk1 is not phosed on T1381 [phosAb]
    *** source: 18635759-Fig-6c
  *** Mekk1[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS "P100-fraction"
    *** times: 0 10+ 30 min
    *** comment: coppt Mekk1 is not ubiq [UbAb]
    *** source: 18635759-Fig-S10

================================================
Does Cd40 relocate to Cyto fraction with Mekk1?
================================================

  *** Cd40[Ab] copptby[WB] Mekk1[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 10+ 30+ min
    *** source: 18635759-Fig-2d
  *** Mekk1[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 10+ 30+ min
    *** source: 18635759-Fig-2c
  *** Mekk1[Ab] copptby[WB] Cd40[Ab] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS "P100 fraction"
    *** times: 0 10++ 30+ min
    *** source: 18635759-Fig-3a

=========================================
Fractionation Stuff does not always hold  or is Fig 5d a typo?
=========================================

  *** Mekk1[Ab] infraction(P100)[WB] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 10+ 30+ 60 min
    *** source: 18635759-Fig-S6
  *** Mekk1[Ab]IP infraction(P100)[WB] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 2 5+ 10+ min
    *** comment: coppt Mekk1 is not phosed on T1381 [phosAb]
    *** source: 18635759-Fig-6e
  *** Mekk1[Ab]IP infraction(P100)[WB] is increased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0 10+ 30 min
    *** source: 18635759-Fig-3b
  *** Mekk1[Ab]IP infraction(P100)[WB] is unchanged irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0+ 2+ 5+ 10+ 30+ min
    *** comment: directly contradicts Fig-3b
    *** source: 18635759-Fig-5d

  *** Mekk1[Ab] infraction(S100)[WB] is decreased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0+++ 5++ 10++ 15++ 30+ 60++ min
    *** source: 18635759-Fig-S7
  *** Mekk1[Ab] infraction(S100)[WB] is decreased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0++ 10+ 30+ 60++ min
    *** source: 18635759-Fig-S6
  *** Mekk1[Ab] infraction(S100)[WB] is decreased irt anti-Cd40 (times)
    *** cells: mSpBCs in BMS
    *** times: 0++ 10+ 30+ min
    *** source: 18635759-Fig-S10
  *** Mekk1[Ab]IP infraction(S100)[WB] is unchanged irt anti-Cd40 (times)
    *** cells: A20 in BMS
    *** times: 0+ 5+ 10+ 30+ min
    *** source: 18635759-Fig-S12

=========
no extras
=========

  *** Cd40[Ab] copptby[WB] Mekk1[Ab] is increased irt anti-Cd40 (10 min)
    *** cells: mSpBCs in BMS
    *** source: 17143273-Fig-4b
  *** Cd40[Ab] copptby[WB] Mekk1[Ab] is increased irt Cd40L (10 min)
    *** cells: mSpBCs in BMS
    *** source: 17143273-Fig-S7a











