===============
Kinase Activity
===============

  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 15+ 30++ 45+++ min
    *** source: 15778223-Fig-1b
  *** xTpl2[tAb]IP IVKA(Mek1)[32P-ATP] is increased irt Lps (times)
    *** cells: RAW2647 in BMS
    *** times: 0 15 30+ 45+ min
    *** source: 15778223-Fig-1a
  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 2 5+ 10++ 15++ 30++ min
    *** source: 15199157-Fig-4
  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 5 10+ 25++ 50++ min
    *** source: 15199157-Fig-7a
  *** Tpl2[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is increased irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** source: 15169888-Fig-9c
  *** Tpl2[Ab]IP IVKA(Mek1,Erks,MBP)[32P-ATP] is increased irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** source: 15485931-Fig-1e
  *** Tpl2[Ab]IP IVKA(Mek1,Erks,MBP)[32P-ATP] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 7+ 15++ 30+ min
    *** source: 15485931-Fig-1a
  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 10+ 20++ 30++ 45+ min
    *** source: 12667451-Fig-6c
  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 15+ 30+ min
    *** source: 12667451-Fig-2b

Nfkb1--

  *** Tpl2[Ab]IP IVKA(Mek1,Erks,MBP)[32P-ATP] is increased irt Lps (15 min)
    *** cells: RAW2647 in BMS
    *** partially inhibited by: xNfkb1(S927A/S932A) [substitution]
    *** source: 15485931-Fig-4d

Tpl2 phos site S400

  *** xTpl2[tAb]IP IVKA(Mek1)(S217/S221)[phosAb] is increased irt Lps (15 min)
    *** cells: RAW2647<xTpl2> in BMS
    *** bkg inhibited by: xTpl2(S400A) [substitution]
    *** source: 17709378-Fig-6d

requires proteasome activity

  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** inhibited by: MG132 [pretreatment]
    *** inhibited by: Lactacystin [pretreatment]
    *** source: 15199157-Fig-8a
  *** Tpl2[Ab]IP IVKA(Mek1,Erks,MBP)[32P-ATP] is increased irt Lps (15 min)
    *** cells: RAW2647 in BMS
    *** partially inhibited by: MG132 [pretreatment]
    *** source: 15485931-Fig-3d
  *** Tpl2[Ab]IP IVKA(Mek1,Erks,MBP)[32P-ATP] is increased irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** partially inhibited by: MG132 [pretreatment]
    *** source: 15485931-Fig-2f

chemicals

  *** Tpl2[Ab]IP IVKA(Mek1)[32P-ATP] is increased irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** inhibited by: PS1145 [pretreatment]
    *** source: 15199157-Fig-3b
  *** Tpl2[Ab]IP IVKA(Mek1,Erks,MBP)[32P-ATP] is increased irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** partially inhibited by: Bay117082 [pretreatment]
    *** source: 15485931-Fig-5e

=======================
Phosphorylation on T290
=======================

Ikk2--

  *** xTpl2[tAb]IP phos(T290)[phosAb] is increased irt Lps (30 min)
    *** cells: RAW2647 in BMS
    *** reqs: Ikk2 [RNAi]
    *** source: 15778223-Fig-6a

chemicals--

  *** xTpl2[tAb]IP phos(T290)[phosAb] is increased irt Lps (30 min)
    *** cells: RAW2647 in BMS
    *** inhibited by: PS1145 [addition]
    *** source: 15778223-Fig-6a

no extras

  *** Tpl2[phosAb] phos(T290)[phosAb] is detectable-but unchanged irt Lps (30 min)
    *** cells: hPBMCs in BMS
    *** source: 15466476-Fig-6a

=======================
Phosphorylation on S400
=======================

  *** xTpl2[tAb]IP phos(S400)[phosAb] is increased irt Lps (times)
    *** cells: RAW2647<xTpl2> in BMS
    *** times: 0 7+ 15+ min
    *** unaffected by: xTpl2(D270A)"KD" [substitution]
    *** source: 17709378-Fig-3a
  *** xTpl2[tAb]IP phos(S400)[phosAb] is increased irt Lps (times)
    *** cells: RAW2647<xTpl2> in BMS
    *** times: 0 7+ 15+ min
    *** unaffected by: xTpl2(T290A) [substitution]
    *** source: 17709378-Fig-3c

chemicals

  *** xTpl2[tAb]IP phos(S400)[phosAb] is increased irt Lps (7 min)
    *** cells: RAW2647<xTpl2> in BMS
    *** unaffected by: BMS345541 [pretreatment]
    *** unaffected by: IKK2InhibitorIV [pretreatment]
    *** source: 17709378-Fig-5c

=====================
Total Phosphorylation
=====================

  *** Tpl2[Ab]IP phos[WBMS-PPase] is increased irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** source: 15485931-Fig-1b

==================
Protein Expression
==================

  *** Tpl2[Ab] prot-exp[WB] is detectable-but unchanged irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0+ 15+ 30+ min
    *** source: 17709378-Fig-1b
  *** xTpl2[Ab] prot-exp[WB] is detectable-but unchanged irt Lps (times)
    *** cells: mBMMs<Nfkb1~null><xTpl2> in BMS
    *** times: 0+ 15+ 30+ min
    *** inhibited by: xTpl2(D270A)"KD" [substitution]
    *** source: 17709378-Fig-1b
  *** xTpl2[Ab] prot-exp[WB] is detectable-but unchanged irt Lps (times)
    *** cells: mBMMs<Nfkb1~null><xTpl2> in BMS
    *** times: 0+ 15+ 30+ min
    *** unaffected by: xTpl2(S400A) [substitution]
    *** unaffected by: xTpl2(S413A) [substitution]
    *** source: 17709378-Fig-2b
  *** Tpl2[Ab] prot-exp[WB] is decreased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0+ 7+ 15+ 30 min
    *** unaffected by: LY294002 [pretreatment]
    *** unaffected by: Wortmannin [pretreatment]
    *** source: 17709378-Fig-5a
  *** xTpl2[tAb]IP prot-exp[WB] is decreased irt Lps (times)
    *** cells: RAW2647 in BMS
    *** times: 0+ 15+ 30 45 min
    *** comment: both xTpl2 splice variants are expressed
    *** comment: decrease is in the p58 band only
    *** source: 15778223-Fig-1a
  *** xTpl2[tAb]IP prot-exp[WB] is decreased irt Lps (times)
    *** cells: RAW2647<xTpl2> in BMS
    *** times: 0++ 15++ 30+ 45 60 min
    *** comment: decrease is in the p58 band only
    *** reversed by: xTpl2(T290A) [substitution]
    *** reversed by: xTpl2(T290D) [substitution]
    *** source: 15699325-Fig-4,5
  *** xTpl2[tAb]IP prot-exp[WB] is decreased irt Lps (times)
    *** cells: RAW2647<xTpl2> in BMS
    *** times: 0++ 15++ 30+ 45 60 min
    *** comment: decrease is in the p58 band only
    *** source: 15699325-Fig-3
  *** Tpl2[Ab] prot-exp[WB] is decreased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0++ 10++ 30++ 60+ min
    *** source: 15169888-Fig-9a
  *** Tpl2[Ab] prot-exp[WB] is decreased irt Lps (60 min)
    *** cells: mBMMs in BMS
    *** reversed by: MG132 [pretreatment]
    *** source: 15169888-Fig-9b
  *** Tpl2[Ab] prot-exp[WB] is detectable-but unchanged irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** source: 15169888-Fig-9d
  *** Tpl2[Ab]IP prot-exp[WB] is decreased irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** source: 15169888-Fig-9c
  *** Tpl2[Ab] prot-exp[WB] is decreased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0++ 7+ 15+ 30 min
    *** reversed by: MG132 [pretreatment]
    *** source: 15485931-Fig-2b
  *** Tpl2[Ab] prot-exp[WB] is decreased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0++ 7++ 15++ 30+ min
    *** source: 15485931-Fig-1c
  *** Tpl2[Ab]IP prot-exp[WB] is decreased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0++ 7++ 15++ 30+ min
    *** source: 15485931-Fig-1a
  *** Tpl2p52[Ab] prot-exp[WB] is decreased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0++ 10++ 20++ 30++ 45++ 60+ min
    *** source: 12667451-Fig-6a
  *** Tpl2p58[Ab] prot-exp[WB] is decreased irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0++ 10++ 20++ 30++ 45+ 60 min
    *** source: 12667451-Fig-6a

======================
Association with Nfkb1
======================

  *** Tpl2[Ab] copptby[WB] Nfkb1[Ab] is detectable
    *** cells: mBMMs in BMS "treated with MG132"
    *** comment: coppt Tpl2 phos(S400)[phosAb] is increased irt Lps (7 min)
    *** source: 17709378-Fig-3b
  *** Tpl2[Ab] copptby[WB] Nfkb1[Ab] is detectable
    *** cells: mBMMs in BMS "treated with MG132"
    *** comment: coppt Tpl2 phos(S400)[phosAb] is increased irt Lps (7 min)
    *** source: 17709378-Fig-5b

=======================================================
Tpl2 is not active when in complex with Nfkb1 and Tnip2
=======================================================

  *** Tnip2[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is unchanged irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** source: 15169888-Fig-9c
  *** Nfkb1[Ab]IP IVKA(Mek1,Erk2,MBP)[32P-ATP] is unchanged irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** source: 15169888-Fig-9c
  *** Nfkb1[Ab]IP IVKA(Mek1,Erks,MBP)[32P-ATP] is unchanged irt Lps (15 min)
    *** cells: mBMMs in BMS
    *** source: 15485931-Fig-1e
  *** Nfkb1[Ab]IP IVKA(Mek1)[32P-ATP] is unchanged irt Lps (times)
    *** cells: mBMMs in BMS
    *** times: 0 10 20 30 45 min
    *** source: 12667451-Fig-6c
