=================
Required Proteins
=================

Nemo--

  *** Ikba[Ab] prot-exp[WB] is decreased irt anti-Cd40 (times)
    *** cells: 70Z3 in BMS
    *** times: 0+ 5+ 10+ 30 60 min
    *** reqs: Nemo [KO]
    *** source: 18635759-Fig-2b

not Traf1--

  *** Ikba[Ab] prot-exp[WB] is decreased irt Cd40L (20 min)
    *** cells: mBMDCs in BMLS
    *** does not req: Traf1 [KO]
    *** source: 12370254-Fig-4b
  *** Ikba[Ab] prot-exp[WB] is decreased irt Cd40L (times)
    *** cells: mBMDCs in BMLS "matured with Cd40L for 18 hr"
    *** times: 0+ 10 30 120+ min
    *** does not req: Traf1 [KO]
    *** source: 12370254-Fig-4b

Traf2--

  *** Ikba[Ab] prot-exp[WB] is decreased irt Cd40L (10 min)
    *** cells: EJ in BMS
    *** reqs: Traf2 [RNAi]
    *** source: 16260598-Fig-3b
  *** Ikba[Ab] prot-exp[WB] is decreased irt Cd40L (times)
    *** cells: mEFs in BMS
    *** times: 0++ 20+ 30+ min
    *** partially reqs: Traf2 [KO]
    *** source: 16260598-Fig-4d
  *** Ikba[Ab] prot-exp[WB] is decreased irt Cd40L (30 min)
    *** cells: HELA<xCd40> in BMS
    *** partially inhibited by: xCd40(T254A)"mT2/T3" [substitution]
    *** inhibited by: xCd40(Q234A/E235A/Q263A/T254A)"mT2/T3/T6" [substitution]
    *** source: 16260598-Fig-2c
  *** Ikba[Ab] prot-exp[WB] is decreased irt anti-Cd40 (times)
    *** cells: A202J in BMS
    *** times: 0++ 5++ 10+ 15 30 min
    *** does not req: Traf2 [null-mut]
    *** source: 12958312-Fig-7a

Traf6--

  *** Ikba[Ab] prot-exp[WB] is decreased irt Cd40L (20 min)
    *** cells: mEFs<Traf6~null><xTraf6> in BMS
    *** reqs: xTraf6 [omission]
    *** source: 16260598-Fig-7b
  *** Ikba[Ab] prot-exp[WB] is decreased irt Cd40L (tnr)
    *** cells: HELA<xCd40(Q234A/E235A)"mT6"> in BMS
    *** reqs: Traf6 [RNAi]
    *** source: 16260598-Fig-8f
  *** Ikba[Ab] prot-exp[WB] is decreased irt Cd40L (30 min)
    *** cells: HELA<xCd40> in BMS
    *** partially inhibited by: xCd40(Q234A/E235A)"mT6" [substitution]
    *** inhibited by: xCd40(Q234A/E235A/Q263A/T254A)"mT2/T3/T6" [substitution]
    *** partially inhibited by: xCd40(Q234A/E235A/Q263A)"mT3/T6" [substitution]
    *** source: 16260598-Fig-2c

Ubc13--

  *** Ikba[Ab] prot-exp[WB] is decreased irt anti-Cd40 (times)
    *** cells: A20 in BMS
    *** times: 0+ 5+ 10+ 30+ 60 min
    *** reqs: Ubc13 [RNAi]
    *** source: 18635759-Fig-2a

Contradictions

  *** Ikba[Ab] prot-exp[WB] is unchanged irt Cd40L (times)
    *** cells: 9HTEO in BMS
    *** times: 0+ 5+ 15+ 30+ min
    *** source: 12122011-Fig-4a

Other

  *** Ikba[Ab] prot-exp[WB] is decreased irt Cd40L (20 min)
    *** cells: mBMDCs in BMLS
    *** unaffected by: Cd40L [pretreatment]
    *** unaffected by: Lps [preconditioning]
    *** source: 12370254-Fig-4b

