=================
Kinase Activation
=================

not Msk1-- or Msk2--

  *** Rsk3[Ab]IP IVKA(peptide)[32P-ATP] is increased irt PMA (10 min)
    *** cells: mEFs in BMLS
    *** does not req: Msk2 [KO]
    *** does not req: Msk1 [KO]
    *** does not req: Msk1 or Msk2 [DKO]
    *** source: 11909979-Fig-4b
  *** Rsk1[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt PMA (times)
    *** cells: mESCs in BMLS
    *** times: 0 10+ 20+ 30+ min
    *** does not req: Msk1 [KO]
    *** source: 11018520-Fig-4a
  *** Rsk2[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt PMA (times)
    *** cells: mESCs in BMLS
    *** times: 0 10+ 20+ 30+ min
    *** does not req: Msk1 [KO]
    *** source: 11018520-Fig-4b
  *** Rsk2[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt PMA (times)
    *** cells: mESCs in BMLS
    *** times: 0 10+ 20+ 30+ min
    *** does not req: Msk1 [KO]
    *** source: 11018520-Fig-4c

Pdpk1--

  *** Rsks[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt PMA (15 min)
    *** cells: mESCs in BMLS
    *** reqs: Pdpk1 [KO]
    *** comment: addition of rPdpk1 to reaction rescues IVKA in Pdpk1-KO cells
    *** source: 10801415-Fig-5a

required features

  *** xRsk1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt PMA (15 min)
    *** cells: COS1<xRsk1> in BMLS
    *** bkg inhibited by: xRsk1(S221A) [substitution]
    *** unaffected by: xRsk1(S359A) [substitution]
    *** bkg inhibited by: xRsk1(S363A) [substitution]
    *** bkg inhibited by: xRsk1(S363E) [substitution]
    *** bkg inhibited by: xRsk1(S380A) [substitution]
    *** partially inhibited by: xRsk1(S380E) [substitution]
    *** bkg inhibited by: xRsk1(T573A) [substitution]
    *** partially inhibited by: xRsk1(T573E) [substitution]
    *** unaffected by: xRsk1(T612A/T621A) [substitution]
    *** unaffected by: xRsk1(S732A) [substitution]
    *** source: 9430688-Fig-6
  *** xRsk1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt PMA (15 min)
    *** cells: COS1<xRsk1> in BMLS
    *** comment: phos sites were shown to be S221 S354 S359 S380 T573 S732 by HPLC
    *** comment: all but phos sites S221 S732 were inhibited by PD98059
    *** source: 9430688-Fig-2

chemicals

  *** Rsks[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt PMA (10 min)
    *** cells: HEK293 in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** unaffected by: SB203580 [pretreatment]
    *** source: 9687510-Fig-5b
  *** Rsk2[Ab]IP IVKA(Rps6)[32P-ATP] is increased irt PMA (15 min)
    *** cells: HEK293E in BMLS
    *** partially inhibited by: FMK [pretreatment]
    *** source: 17360704-Fig-4b
  *** Rsks[Ab]IP IVKA(Crosstide)[32P-ATP] is increased irt PMA (10 min)
    *** cells: mESCs in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** source: 11018520-Fig-2a

no extras

  *** xRsk1[tAb]IP IVKA(peptide)[32P-ATP] is increased irt PMA (times)
    *** cells: COS1<xRsk1> in BMLS
    *** times: 0 2 5+ 10++ 15++ 20++ 25++ 30++ 40++ min
    *** source: 9430688-Fig-1b
  *** xRsk1[GST]IP IVKA(Crosstide)[32P-ATP] is increased irt PMA (15 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** source: 9687510-Fig-3a
  *** Rsk1[Ab]IP IVKA(auto)[32P-ATP] is increased irt PMA (tnr)
    *** cells: SAOS2 in BMS
    *** source: 15073170-Fig-5a
  *** Rsk1[Ab]IP IVKA(peptide)[32P-ATP] is increased irt PMA (times)
    *** cells: COS1 in BMLS
    *** times: 0 2 5+ 10++ 15++ 20++ 25++ 30++ 40++ min
    *** source: 9430688-Fig-1a
  *** Rsk1[Ab]IP IVKA(Rela)[32P-ATP] is increased irt PMA (tnr)
    *** cells: SAOS2 in BMS
    *** source: 15073170-Fig-5a
  *** Rsk3[Ab]IP IVKA(peptide)[32P-ATP] is increased irt PMA (10 min)
    *** cells: mEFs in BMLS
    *** inhibited by: PD184352 [addition] no-bkg-control
    *** unaffected by: SB203580 [addition] no-bkg-control
    *** source: 11909979-Fig-3b

===============
Phosphorylation
===============

not Pdpk1--

  *** Rsks[Ab] phos[WBMS] is increased irt PMA (15 min)
    *** cells: mESCs in BMLS
    *** does not req: Pdpk1 [KO]
    *** source: 10801415-Fig-5a

  *** Rsk1[phosAb] phos(S380)[phosAb] is increased irt PMA (15 min)
    *** cells: HEK293 in BMS
    *** inhibited by: U0126 [pretreatment]
    *** inhibited by: Bisindolylmaleimide-I [pretreatment]
    *** unaffected by: Wortmannin [pretreatment]
    *** unaffected by: Rapamycin [pretreatment]
    *** source: 15342917-Fig-1b
  *** Rsk1[phosAb] phos(sitenr)[phosAb] is increased irt PMA (times)
    *** cells: SAOS2 in BMS
    *** times: 0 10+ 20+ min
    *** source: 15073170-Fig-3c
  *** xRsk1[tAb]IP phos(S363)[phosAb] is increased irt PMA (15 min)
    *** cells: COS1<xRsk1> in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** source: 9430688-Fig-5a
  *** xRsk1[tAb]IP phos(T359)[phosAb] is increased irt PMA (15 min)
    *** cells: COS1<xRsk1> in BMLS
    *** inhibited by: PD98059 [pretreatment]
    *** source: 9430688-Fig-5a
  *** xRsk1[tAb]IP phos(T380)[phosAb] is increased irt PMA (15 min)
    *** cells: COS1<xRsk1> in BMLS
    *** bkg inhibited by: PD98059 [pretreatment]
    *** source: 9430688-Fig-5a

  *** xRsk1[GST]IP phos[MS] is increased irt PMA (15 min)
    *** cells: HEK293<xRsk1> in BMLS
    *** source: 9687510-Fig-3a


========
Location
========

  *** xRsk1[tAb] locatedin(cytoplasm)[EM] is detectable-but unchanged irt PMA (10 min)
    *** cells: HEK293<xMsk1> in BMLS
    *** source: 9687510-Fig-7b
