=====================================================================
endogenous Atf2 location depends on the amount of Jun in the cell
=====================================================================

high Jun conc

  *** Atf2[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-3c
  *** Atf2[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: MCF7 in BMS
    *** source: 16511568-Fig-3c

Jun knock-down

  *** Atf2[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: MCF7 in BMS
    *** partially reqs: Jun [RNAi]
    *** source: 16511568-Fig-3d
  *** Atf2[Ab] locatedin(nucleus)[IHC] is detectable
    *** cells: MCF7 in BMS
    *** partially reqs: Jun [RNAi]
    *** source: 16511568-Fig-S4

low Jun conc

  *** Atf2[Ab] locatedin(cytoplasm)[IHC] is detectable
    *** cells: F9 in BMS
    *** source: 16511568-Fig-3c

===============================================
over-expressed Atf2 is located in the cytoplasm
===============================================

  *** xAtf2[XFP] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS1 in BMS
    *** comment: xAtf2 is a dimer [BiFC]
    *** source: 16511568-Fig-1a
  *** xAtf2[XFP] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS1 in BMS
    *** enhanced by: xAtf2(400-415)"NES" [substitution]
    *** source: 16511568-Fig-2a
  *** xAtf2[XFP] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-1a
  *** xAtf2[XFP] locatedin(cytoplasm)[IHC] is unchanged itpo xE1a!Ade05
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-4d
  *** xAtf2[XFP] locatedin(cytoplasm)[IHC] is unchanged itpo xEp300
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-4d
  *** xAtf2[XFP] locatedin(cytoplasm)[IHC] is unchanged itpo xFos
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-4d
  *** xAtf2[XFP] locatedin(cytoplasm)[IHC] is unchanged itpo xRela
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-4d
  *** xAtf2[tAb] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-S2
  *** xAtf2[tAb] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS1 in BMS
    *** unaffected by: xJnk? [addition]
    *** unaffected by: xP38? [addition]
    *** unaffected by: SP600125 [addition]
    *** unaffected by: SB203580 [addition]
    *** source: 16511568-Fig-S5A,S5B

=====================================================================
over-expressed Atf2 is located in the nucleus itpo
over-expressed Jun, Junb, or Jund
=====================================================================

  *** xAtf2[tAb] locatedin(nucleus)[IHC] is increased itpo xJun
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-S2
  *** xAtf2[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: COS1<xJun> in BMS
    *** comment: xAtf2 is dimerized with xJun [BiFC]
    *** source: 16511568-Fig-1a
  *** xAtf2[XFP] infraction(nuclear)[WB] is increased itpo xJun
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-S3
  *** xAtf2[XFP] locatedin(nucleus)[IHC] is increased itpo xJun
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-4d
  *** xAtf2[XFP] locatedin(nucleus)[IHC] is increased itpo xJunb
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-4d
  *** xAtf2[XFP] locatedin(nucleus)[IHC] is increased itpo xJund
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-4d

Jun must be capable of dimerizing with Atf2 but Jun phos state does not matter

  *** xAtf2[XFP] locatedin(nucleus)[IHC] is increased itpo xJun
    *** cells: COS1 in BMS
    *** unaffected by: xJun(S63A/S73A) [substitution]
    *** inhibited by: xJun(mnr)"dimerization-mutant" [substitution]
    *** unaffected by: xJun(124-331) [substitution]
    *** source: 16511568-Fig-3a
  *** xAtf2(T69A/T71A)[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: COS1<xJun> in BMS
    *** unaffected by: xJun(S63A/S73A) [substitution]
    *** source: 16511568-Fig-S5C

Atf2 phos state does not matter

  *** xAtf2[XFP] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS1 in BMS
    *** unaffected by: xAtf2(T69A/T71A) [substitution]
    *** unaffected by: xAtf2(T69D/T71D) [substitution]
    *** source: 16511568-Fig-3a
  *** xAtf2(T69A/T71A)[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: COS1<xJun> in BMS
    *** unaffected by: xAtf2(T69D/T71D) [substitution]
    *** comment: xAtf2(T69A/T71A) is dimerized with xJun [BiFC]
    *** source: 16511568-Fig-S5C

=================
Molecular Details
=================

  *** xAtf2(342-505)[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: COS1<xCrm1(566-720)> in BMS
    *** comment: xAtf2(342-505) is dimerized with xCrm1(566-720) [BiFC]
    *** inhibited by: xJun [addition]
    *** inhibited by: xAtf2((342-505)/V405A/L408A/L411A/L413A) [substitution]
    *** source: 16511568-Fig-4e
  *** xAtf2(del(356-372))[XFP] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS1 in BMS
    *** unaffected by: xJun [addition]
    *** source: 16511568-Fig-4a
  *** xAtf2(del(356-372))[XFP] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS1<xJun> in BMS
    *** comment: xAtf2 is dimerized with xJun [BiFC]
    *** source: 16511568-Fig-4a
  *** xAtf2(del(342-358))[XFP] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS1 in BMS
    *** partially inhibited by: xAtf2(del(342-372)) [substitution]
    *** source: 16511568-Fig-2d
  *** xAtf2(del(356-372))[XFP] locatedin(cytoplasm)[IHC] is detectable
    *** cells: COS1 in BMS
    *** partially inhibited by: xAtf2(del(342-372)) [substitution]
    *** source: 16511568-Fig-2d



  *** xAtf2(V405A/L408A/L411A/L413A)"NES-mut"[XFP] locatedin(nucleus)[IHC] is detectable
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-2a

  *** xAtf2[XFP] locatedin(nucleus)[IHC] is increased irt LMB (12 hr)
    *** cells: COS1 in BMS
    *** source: 16511568-Fig-2a


rule 540 implies that Atf2 is recruited into the nucleus by Jun.
It is more likely that Atf2 (which contains both NES and NLS)
is shuttling in and out of the nucleus and is prevented from leaving
nucleus by dimerization with Jun.











