===============
Phosphorylation
===============

S15

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt Adriamycin (24 hr)
    *** cells: U2OS in BMS
    *** source: 12397362-Fig-1a

S15/S20/S26

  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: MCF7 in BMS
    *** times: 0 1+ 2+ hr
    *** comment: new protein is phosed on S15 and S20 [phosAb]
    *** comment: both prot-exp and phos is unaffected by pretreatment with Rottlerin
    *** source: 16377624-Fig-1a
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: MCF7 in BMS
    *** times: 0 7 15 30 60 120+ 240++ 480++ 720++ 1440++ min
    *** comment: new Tp53 protein is phosed on S15 [phosAb]
    *** comment: new Tp53 protein is phosed on S20 [phosAb]
    *** comment: new Tp53 protein is phosed on S26 [phosAb]
    *** source: 11030628-Fig-5b

S46

  *** xTp53[phosAb] phos(S46)[phosAb] is increased irt Adriamycin (times)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** times: 0 6+ 12+ 18+ 24+ hr
    *** enhanced by: xTp53(P72R) [substitution]
    *** source: 17906639-Fig-6d

===========
Acetylation
===========

K373/K382

  *** xTp53[Ab] acetyl(K373/K382)[AcAb] is increased irt Adriamycin (times)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** times: 0 3 6 12 18+ 24+ hr
    *** enhanced by: xTp53(P72R) [substitution]
    *** source: 17906639-Fig-S6a

===========
Methylation
===========

  *** Tp53[Ab]IP methyl(K372)[MeAb] is increased irt Adriamycin (3 hr)
    *** cells: U2OS in BMS "nuclear-extracts"
    *** reqs: Setd7 [RNAi]
    *** comment: normalized to total Tp53 
    *** source: 15525938-Fig-3e
  *** Tp53[Ab]IP methyl(K372)[MeAb] is increased irt Adriamycin (tnr)
    *** cells: 293F in BMS "nuclear-extracts"
    *** source: 15525938-Fig-3d

===============================
Association with other proteins
===============================

Pin1--

  *** Tp53[Ab] snaggedby[WB] rPin1[GST] is increased irt Adriamycin (24 hr)
    *** cells: U2OS in BMS
    *** comment: snagged Tp53 is phosed on S15 [phosAb]
    *** source: 12397362-Fig-1a

Pkcd--

  *** Pkcd[Ab] copptby[WB] Tp53[Ab] is increased irt Adriamycin (times)
    *** cells: MOLT4 in BMS
    *** times: 0 8 24+ hr
    *** source: 16377624-Fig-3b
  *** Tp53[Ab] copptby[WB] Pkcd[Ab] is increased irt Adriamycin (times)
    *** cells: MOLT4 in BMS
    *** times: 0+ 8+ 24++ hr
    *** source: 16377624-Fig-3a

==================
Protein Expression
==================

Atm--

  *** Tp53[Ab] prot-exp[WB] is unchanged irt Adriamycin (times)
    *** cells: GM719<Atm~fmut> in BMS
    *** times: 0 1 4 6 21 hr
    *** source: 11459832-Fig-3a
  *** Tp53[Ab] prot-exp[WB] is unchanged irt Adriamycin (times)
    *** cells: GM3332<Atm~fmut> in BMS
    *** times: 0 1 4 6 21 hr
    *** source: 11459832-Fig-3a
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: GM1526<Atm~fmut> in BMS
    *** times: 0 1 4 6 21+ hr
    *** source: 11459832-Fig-3a

Pkcd--

  *** xTp53[tAb]IP prot-exp[WB] is increased irt Adriamycin (24 hr)
    *** cells: HEK293T<xTp53> in BMS
    *** comment: new protein is phosed on S46 [phosAb]
    *** comment: both prot-exp and phos is inhibited by addition of xPkcd(mnr)"KD"
    *** source: 16377624-Fig-2b
  *** Tp53[Ab]IP prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: U2OS in BMS
    *** times: 0 8+ 24+ hr
    *** comment: new protein is phosed on S46 [phosAb]
    *** comment: both prot-exp and phos partially req Pkcd [RNAi]
    *** source: 16377624-Fig-2c
  *** Tp53[Ab]IP prot-exp[WB] is detectable-but unchanged irt Adriamycin (tnr)
    *** cells: 293F in BMS "nuclear-extracts"
    *** source: 15525938-Fig-3d

Pin1--  ???

  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (tnr)
    *** cells: U2OS in BMS
    *** unaffected by: xPin1 [addition]
    *** source: 12397361-Fig-3a
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: mEFs<Pin1~null> in BMS
    *** times: 0 4 8+ 24++ hr
    *** comment: prot-exp is increased at 4 hr and decreased at 24 hr itpo xPin1
    *** source: 12397361-Fig-3c
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: mEFs<Pin1~null> in BMS
    *** times: 0 2+ 4+ 8+ 16++ 24++ hr
    *** source: 12397361-Fig-3b

not Setd7--

  *** Tp53[Ab]IP prot-exp[WB] is increased irt Adriamycin (3 hr)
    *** cells: U2OS in BMS "nuclear-extracts"
    *** does not req: Setd7 [RNAi]
    *** source: 15525938-Fig-3e

Tp53 features

  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: mEFs in BMS
    *** times: 0 6+ 12+ 24 hr
    *** enhanced by: kTp53(S18A/S23A) [knockin]
    *** source: 16757976-Fig-2a

Chemicals

  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: NIH3T3 in BMS
    *** times: 0 1+ 2+ 3+ 4++ hr
    *** partially inhibited by: Tunicamycin [pretreatment]
    *** source: 14744935-Fig-S1b
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: WI38 in BMS
    *** times: 0+ 3++ 6+++ hr
    *** partially inhibited by: Tunicamycin [cotreatment]
    *** partially inhibited by: Thapsigargin [cotreatment]
    *** source: 14744935-Fig-2a
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: MCF7 in BMS
    *** times: 0 8+ 24++ hr
    *** inhibited by: Rottlerin [pretreatment]
    *** source: 16377624-Fig-4c
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: MCF7 in BMS
    *** times: 0 3 6+ 9+ hr
    *** unaffected by: MG132 [cotreatment]
    *** source: 12963717-Fig-1g
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: MCF7 in BMS
    *** times: 0 3+ 6+ 9+ hr
    *** unaffected by: MG132 [cotreatment]
    *** source: 12963717-Fig-1g
  *** Tp53[Ab]IP prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: MCF7 in BMS
    *** times: 0 8+ 24++ hr
    *** comment: new protein is phosed on S46 at 24 hr only [phosAb]
    *** comment: both prot-exp and phos is inhibited by pretreatment with Rottlerin
    *** source: 16377624-Fig-1b
  *** xTp53[tAb]IP prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: HEK293T<xTp53> in BMS
    *** times: 0 8+ 24++ hr
    *** comment: new protein is phosed on S46 at 24 hr only [phosAb]
    *** comment: both prot-exp and phos is inhibited by pretreatment with Rottlerin
    *** source: 16377624-Fig-1c

no extras

  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (24 hr)
    *** cells: U2OS in BMS
    *** source: 12397362-Fig-1a
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: mEFs in BMS
    *** times: 0 2+ 4++ 8++ 16++ 24+ hr
    *** source: 12397361-Fig-3b
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: mEFs in BMS
    *** times: 0 4+ 8++ 24+ hr
    *** source: 12397361-Fig-3c
  *** Tp53[Ab]IP prot-exp[WB] is unchanged irt Adriamycin (times)
    *** cells: MOLT4 in BMS
    *** times: 0+ 8+ 24+ hr
    *** source: 16377624-Fig-3a
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: GM2184 in BMS
    *** times: 0 1 4+ 6++ 21+++ hr
    *** source: 11459832-Fig-3a
  *** Tp53[Ab] prot-exp[WB] is increased irt Adriamycin (times)
    *** cells: GM3382 in BMS
    *** times: 0 1 4+ 6++ 21+++ hr
    *** source: 11459832-Fig-3a

