===============================
Increase in Protein Expression
===============================

  *** xTp53[Ab] prot-exp[WB] is increased irt Etoposide (6 hr)
    *** cells: H1299<xCop1><xTp53> in BMS
    *** inhibited by: xCop1(S387A) [substitution]
    *** source: 16931761-Fig-4f

============================
Acetylation on K373 and K382
============================

Pin1--

  *** Tp53[AcAb] acetyl(K373)[AcAb] is increased irt Etoposide (12 hr)
    *** cells: HCT116 in BMS
    *** reqs: Pin1 [RNAi]
    *** source: 17906639-Fig-4d
  *** Tp53[AcAb] acetyl(K382)[AcAb] is increased irt Etoposide (12 hr)
    *** cells: HCT116 in BMS
    *** reqs: Pin1 [RNAi]
    *** source: 17906639-Fig-4d

not Ppp1r13l--

  *** xTp53[Ab]IP acetyl[KAcAb] is increased irt Etoposide (12 hr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** does not req: Ppp1r13l [RNAi]
    *** source: 17906639-Fig-S8c

no extras--

  *** Tp53[AcAb] acetyl(K382)[AcAb] is increased irt Etoposide (12 hr)
    *** cells: U2OS in BMS
    *** comment: increase partially correlates with the increase in Tp53 prot-exp
    *** source: 17906639-Fig-S1a

======================
Phosphorylation on S15
======================

  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt Etoposide (12 hr)
    *** cells: U2OS in BMS
    *** comment: increase partially correlates with the increase in Tp53 prot-exp
    *** source: 17906639-Fig-S1a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt Etoposide (times)
    *** cells: C3ABR in BMS
    *** times: 0 90+ 150+ 210+ min
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-1a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt Etoposide (times)
    *** cells: L3AT<Atm~fmut> in BMS
    *** times: 0 90+ 150++ 210++ min
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-1a
  *** Tp53[phosAb] phos(S15)[phosAb] is increased irt Etoposide (times)
    *** cells: MCF7<xMdm4> in BMS
    *** times: 0 5++ 7+ hr
    *** comment: no control run for total Tp53 protein
    *** source: 15788536-Fig-1b


==========
ChIP assay
==========

  *** Tp53[Ab] boundto(Bax-gene)[ChIP] is increased irt Etoposide (12 hr)
    *** cells: HCT116 in BMS "treated with Nutlin3a"
    *** partially reqs: Pin1 [RNAi]
    *** source: 17906639-Fig-1c
  *** Tp53[Ab] boundto(Cdkn1a-gene)[ChIP] is increased irt Etoposide (12 hr)
    *** cells: HCT116 in BMS
    *** comment: both total and bound Tp53 is decreased itao Pin1 [RNAi]
    *** source: 17906639-Fig-1b
  *** Tp53[Ab] boundto(Cdkn1a-gene)[ChIP] is increased irt Etoposide (12 hr)
    *** cells: HCT116 in BMS "treated with Nutlin3a"
    *** reqs: Pin1 [RNAi]
    *** source: 17906639-Fig-1c
  *** Tp53[Ab] boundto(Cdkn1a-gene)[ChIP] is increased irt Etoposide (12 hr)
    *** cells: U2OS in BMS
    *** comment: increase correlates with the increase in Tp53 prot-exp
    *** source: 17906639-Fig-S1a
  *** Tp53[Ab] boundto(Cdkn1a-gene)[ChIP] is increased irt Etoposide (times)
    *** cells: HCT116 in BMS
    *** times: 0 5+ 8++ 12++ hr
    *** comment: not normalized for increased Tp53 prot-exp
    *** source: 17906639-Fig-2
  *** xTp53{acetyl(K373/K382)}[AcAb] boundto(Cdkn1a-gene)[ChIP] is increased irt Etoposide (12 hr)
    *** cells: H1299<Tp53~null><xTp53> in BMS
    *** comment: normalized for Tp53 prot but not acetyl
    *** reqs: Pin1 [RNAi]
    *** inhibited by: PIB [pretreatment]
    *** source: 17906639-Fig-5c
  *** xTp53{acetyl(K373/K382)}[AcAb] boundto(Cdkn1a-gene)[ChIP] is increased irt Etoposide (12 hr)
    *** cells: SAOS2<Tp53~null><xTp53> in BMS
    *** comment: not normalized for Tp53 prot or acetyl
    *** partially inhibited by: xTp53(T81A) [substitution]
    *** source: 17906639-Fig-6b
