|
|
 | used in 10608883 with no explanation except that they are fibroblasts
|
|
|
|
| NIH3T3 expressing InsR 12606547(C)
|
|
|
|
| http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=88062428&collection=ecacc_gc
|
|
|
|
| TIB-208 mouse lymphoblast suspension B lymphocyte; reticulum cell sarcoma
|
|
|
|
| do not express IL12Rb1 mRNA 9768751-Fig-3
|
|
|
|
| do not express IL12Rb2 mRNA 9768751-Fig-3
|
|
|
|
 | The diploid mouse B cell line A20.2J (27) was the gift of Dr. David McKean (Mayo Clinic, Rochester, MN). 12958312(C)
|
|
|
|
| 27. Kim, K. J., Kanellopoulos-Langevin, C., Merwin, R. M., Sachs, D. H., and
Asofsky, R. (1979) J. Immunol. 122, 549–554
|
|
|
|
| A20T2-\-: A202J<Traf2~null> 12958312(D)
|
|
|
|
| A-375 CRL-1619 human skin; malignant melanoma adherent
|
|
|
|
| contains Braf(V600E) 19274086(C)
|
|
|
|
| super metastatic human melanoma cells 11278329(C)
|
|
|
|
| A427 HTB-53 human epithelial adherent lung; carcinoma
|
|
|
|
| contains a Kras-CA-mutation 16630891(C)
|
|
|
|
| ATCC HTB-44 kidney carcinoma adherent
|
|
|
|
| CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.0? c.1_522del522 Homozygous
SETD2 p.V2033fs*9 c.6098_6099delTG Homozygous
VHL p.G144fs*14 c.426_429delTGAC Homozygous
|
|
|
|
| breast carcinoma cell line
|
|
|
|
| A431 CRL-1555 human epithelial adherent epidermis; epidermoid carcinoma
|
|
|
|
| expresses moderate levels of Pten mRNA 9187108(D)
|
|
|
|
| Egf resistant clone of A431 17646046(C)
|
|
|
|
| number of EgfR: WT > 11a > 1a > 8a
|
|
|
|
| human alveolar type II (ATII) epithelial cell-derived cell line 17088343(C)
|
|
|
|
| A549 CCL-185 human lung; carcinoma epithelial adherent
|
|
|
|
| CDKN2A p.M1_*157del c.1_471del471 Homozygous
KRAS p.G12S c.34G>A Homozygous
STK11 p.Q37* c.109C>T Homozygous
|
|
|
|
| Samples from same individual: A549SF
|
|
|
|
| mRNA: Fzd1-low Fzd2-pos Fzd3-neg Fzd4-neg Fzd5-low Fzd6-neg Fzd7-low Fzd8-pos Fzd9-neg Fzd10-neg 18215320-Fig-2
|
|
|
|
| mRNA: Ror1-pos Ror2-neg 18215320-Fig-2
|
|
|
|
| mRNA: Lrp5-pos Lrp-6-pos 18215320-Fig-2
|
|
|
|
| A-673 CRL-1598 human fibroblast adherent muscle; rhabdomyosarcoma
|
|
|
|
| A7r5 CRL-1444 rat fibroblast adherent thoracic aorta; smooth muscle
|
|
|
|
| a human melanoma line unusually rich in NGF receptor (4) 6093111(C)
|
|
|
|
| 4. Fabricant, R.N., DeLarco, J.E. & Todaro,G.J. (1977) Proc. Nati. Acad. Sci. USA 74, 565-569.
|
|
|
|
| The murine H-2b-specific CTL clones 11 and AB.1 have been described previously (15). Cells were stimulated weekly with irradiated C57BL/6J spleen cells in media supplemented with interleukin-2, and experiments were performed 4–6 days after stimulation.
|
|
|
|
| 15. Kane, K. P., Sherman, L. A., and Mescher, M. F. (1989) J. Immunol. 142,
4153– 4160
|
|
|
|
| adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Organ: kidney
Disease: renal cell adenocarcinoma
Derived from metastatic site: pleural effusion
|
|
|
|
| Growth is inhibited by human interferon [Hogan, T. F., personal communication]. ACHN may be of use for antiproliferative studies using human interferons or interferon inducers.
|
|
|
|
| CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.? c.317_522del206 Homozygous
NF2 p.R57* c.169C>T Homozygous
|
|
|
|
| human B lymphoblastoid EBV-transformed cell line 2504877(C)
|
|
|
|
| Van De Griend, R .J.,B.A .Krimpen, C .P M .Ranfeltap, and R .H .Bolhuis.1984. Rapidlyexpanded activatedhuman killercloneshavestrongantitumorcellactivityand have surface phenotype of either T, nonT, or null cells.J . Immunol. 132:3185.
|
|
|
|
| used without explanation in 9697832
|
|
|
|
| Untransformed skin fibroblasts from a patient with AT
|
|
|
|
| http://ccr.coriell.org/Sections/Search/Sample_Detail.aspx?Ref=AG04405&PgId=166
|
|
|
|
| normal human fibroblasts 21149446(C)
|
|
|
|
| murine macrophage cell line 17277110
|
|
|
|
| myeloma cell line 9020138(C)
|
|
|
|
| a human B cell line 8995252(C)
|
|
|
|
| human thyroid cancer cells from GF Juillard (University of California at Los Angeles) 14729949(C)
|
|
|
|
| express high level of Rela protein but low levels of Pten 14729949(D)
|
|
|
|
| human thyroid tumor from anaplastic carcinoma 15231710(C)
|
|
|
|
| 20. Russo D, Arturi F, Bulotta S, Pellizzari L, Filetti S, Manzini G, Damante G, Tell G 2001 ApeI/Ref-I expression and cellular localization in human thyroid carcinoma cell lines. J Endocrinol Invest 24:C10–C12.
|
|
|
|
| ARPE-19 CRL-2302 human epithelial adherent eye (retina), pigmented epithelium
|
|
|
|
| AsPC-1 CRL-1682 human ascites (metastasis); adenocarcinoma adherent (pancreas primary)
|
|
|
|
| Lymphoblastoid cells established from a patient with AT 16858402(C).
|
|
|
|
| ATIABR cells contain near-full-length ATM, homozygous for 7636del 9. This is a kinase dead mutant that expresses a less stable form of ATM. 16858402(C)
|
|
|
|
| an immortalized A-T cell line, AT22IJE-T (10) 9733514(C)
|
|
|
|
| Y. Ziv et al., Oncogene 15, 159 (1997).
|
|
|
|
| Immortalized AT fibroblasts 19001589(C)
|
|
|
|
| express Brca1 protein 10550055-Fig-1c
|
|
|
|
| Lymphoblastoid cells established from a patient with AT 16858402(C)
|
|
|
|
| AT25ABR cells do not express Atm 16858402-Fig-3
|
|
|
|
| primary fibroblasts from a patient with AT 15788536(C)
|
|
|
|
| Atm deficient lymphoblasts 15073328(C)
|
|
|
|
| SV40-transformed A-T fibroblast cell line 15234984(C)
|
|
|
|
| ATM-deficient cell line 20026654(C)
|
|
|
|
| Primary fibroblasts from a patient with ataxia-telangiectasia 14695167(C)
|
|
|
|
| authors site Coriell as source but they do not list it
|
|
|
|
| Atm deficient lymphoblasts 15073328(C) 15788536(C)
|
|
|
|
| Chondrogenic cell line derivied by mouse teratocarcinoma
|
|
|
|
| In culture, these cells mimic the multistep process of chondrocyte differentiation. After plating, they proliferate until confluency, at which point they undergo growth arrest following contact inhibition. After 4 days, these cells undergo a phenotypic change characterized by the secretion of a variety of ECM molecules, such as type II collagen, aggrecan, and link protein, and by the formation of cartilaginous nodules on the culture plate (12, 18, 19).
|
|
|
|
| 12. Shukunami, C., Ohta, Y., Sakuda, M., and Hiraki, Y. (1998) Exp. Cell Res. 241, 1–11
|
|
|
|
| 18. Atsumi, T., Miwa, Y., Kimata, K., and Ikawa, Y. (1990) Cell Differ. Dev. 30, 109 –116
|
|
|
|
| 19. Shukunami, C., Shigeno, C., Atsumi, T., Ishizeki, K., Suzuki, F., and Hiraki, Y. (1996) J. Cell Biol. 133, 457– 468
|
|
|
|
| cells deficient in Mre11; hypomorphic for Mre11 mutations 21149446(C)
|
|
|
|
| expresses normal levels of Atm and Rad50 protein but greatly reduced levels of Mre11 Nbs1 21149446-Fig-6b
|
|
|
|
| Lymphoblastoid cell line from a patient with ATLD 16858402(C)
|
|
|
|
| expresses normal levels of Atm protein but greatly reduced levels of Mre11 Rad50 Nbs1 16858402-Fig-4b
|
|
|
|
| melanoma cells 15572665(C)
|
|
|
|
| B16-F1 CRL-6323* mouse adherent skin; melanoma
|
|
|
|
| contains WT Braf 19274086(C)
|
|
|
|
| B16-F10 CRL-6475 mouse adherent skin melanoma
|
|
|
|
| B5/589 human mammary epithelial cell line that is particularly sensitive to the mitogenic effects of Hgf 1846706(C)
|
|
|
|
| Rubin JS et al PNAS 88, 415 (1991)
|
|
|
|
| a cytotoxic T-cell line 11486019(C)
|
|
|
|
| 55. Sekaly, R. P., H. R. MacDonald, P. Zaech, and M. Nabholz. 1982. Cell cycle regulation of cloned cytolytic T cells by T cell growth factor: analysis by flow microfluorometry. J. Immunol. 129:1407–1414.
|
|
|
|
| used without explanation in 99950686(C)
|
|
|
|
| used without explanation in 15039778(C)
|
|
|
|
| a macrophage cell line 16537926(C)
|
|
|
|
| derived from SV40-transformed mature splenic macrophages 10799548(C)
|
|
|
|
| 31. Morgan, C., Pollard, J. W., and Stanley, E. R. (1987) J. Cell. Physiol. 130, 420 – 427
32. Buscher, D., Sbarba, P. D., Hipskind, R. A., Rapp, U. R., Stanley, E. R., and Baccarini, M. (1993) Oncogene 8, 3323–3332
|
|
|
|
| BAC1.2F5 is a simian virus 40 large-T-antigen-immortalized cell line that retains CSF-1 dependence and many other properties of primary murine BMM. 9632768(C)
|
|
|
|
| BA/F3 mouse pro B cells DSMZ#ACC-300
|
|
|
|
| IL-3 dependent murine pro B cell line established from peripheral blood; apparently derived from BALB/c mouse
|
|
|
|
| W4E9 = BAF3<xIL2Ra><xIL2Rb>
|
|
|
|
| a subclone of BAF3 1535827(C)
|
|
|
|
| a bone marrow derived muring IL3 dependent pro-B cell line 1535827(C)
|
|
|
|
| Palacios, R., and Steinmetz, M. (1985). IL-3dependent mouse clones that express B-220 surface antigen, contain lg genes in germ-line configuration, and generate B lymphocytes in vivo. Cell 47, 727-734.
|
|
|
|
| It exhibits the following profile of cell surface marker expression:
|
|
|
|
| Mac-l+ Bpl- B220- slgM- Lybl- Thyl- Ly1- L3T4(CD4)- Lyt2(CD8) IL-2Ra+
|
|
|
|
| [2590941] Hatakeyama, M., Mori, H., Doi, T.. and Taniguchi, T. (1989). A restricted cytoplasmic region of IL-2 receptor beta chain is essential for growth signal transduction but not for ligand binding and internalization. Cell 59, 837-845.
|
|
|
|
| a subline of the IL3-dependent murine hematopeoietic cell line BAF/3 which endogenously expressed the IL2Ra and IL2Rgc but not IL2Rbc chain (23) 7568005(C)
|
|
|
|
| Hatakeyama, M., Mori, H., Doi, T.. and Taniguchi, T. (1989). A restricted cytoplasmic region of IL-2 receptor beta chain is essential for growth signal transduction but not for ligand binding and internalization. Cell 59, 837-845.
|
|
|
|
| does not express IL6R 1662392(C)
|
|
|
|
| expresses Jak2 protein 8134389-Fig-3c
|
|
|
|
| F7: BAFB03<IL2Rb~null><xIL2Rb>
|
|
|
|
| 14B: BAFB03<IL2Rb~null><xIL2Rb>
|
|
|
|
| express Lyn Fyn not Src Lck Fgr Yes 8483935-Fig-3
|
|
|
|
| A15: BAFB03<IL2Rb~null><xIL2Rb(del(312-383))>
|
|
|
|
| PA2: BAFB03<IL2Rb~null><xIL2Rb(P257S/P259S)>
|
|
|
|
| S25: BAFB03<IL2Rb~null><xIL2Rb(del(266-323))>
|
|
|
|
| FJM13: BAFB03<IL2Rb~null><xIL2Rb><xJak3(mnr)"DN">
|
|
|
|
| FJ36: BAFB03<IL2Rb~null><xIL2Rb><xJak3>
|
|
|
|
| BAFh130: BAFB03<IL2Rb~null><xIL6st>
|
|
|
|
| FWT2: BAFB03<IL2Rb~null><xIL2Rb>
|
|
|
|
| Kawahara A Mol Cell Biol 14:5433 (1994)
|
|
|
|
| BALB/3T3 cloneA31 CCL-163 mouse fibroblast adherent skeletal muscle; lysyl oxidase deficiency
|
|
|
|
| murine keratinocytes from Harold L Moses [13] 14993291(C)
|
|
|
|
| 13. Coffey, R. J., Jr., C. C. Bascom, N. J. Sipes, R. Graves-Deal, B. E. Weissman and H. L. Moses. 1988. Selective inhibition of growth-related gene expression in murine keratinocytes by transforming growth factor beta. Mol. Cell Biol. 8:3088–3093.
|
|
|
|
| The BCC cell line originally named BCC-1/KMC was established from human BCC derived from the undi¡erentiated type of BCC tumor arising on a thermal traumatic scar. 12445202(C)
|
|
|
|
| EBV-transformed B cell line provided by ... 8609418(C)
|
|
|
|
| BCL1 clone 3 used in 2295790
|
|
|
|
| BCL1 clone 5B1b TIB-197 mouse B lymphocyte; leukemia; lymphoma lymphoblast adherent
|
|
|
|
| human thyroid tumor from papillary carcinoma 15231710(C)
|
|
|
|
| 20. Russo D, Arturi F, Bulotta S, Pellizzari L, Filetti S, Manzini G, Damante G, Tell G 2001 ApeI/Ref-I expression and cellular localization in human thyroid carcinoma cell lines. J Endocrinol Invest 24:C10–C12.
|
|
|
|
| BRAF p.V600E c.1799T>A Homozygous
TP53 p.D259Y c.775G>T Homozygous
|
|
|
|
| BEAS2B CRL-9609 human epithelial adherent lung (bronchus); virus transformed
|
|
|
|
| betaHC-9: a tissue culture model of pancreatic beta-cell 15192046(C)
|
|
|
|
| expressed EgfR and ErbB4 15192046(C)
|
|
|
|
| used without explanation in: 8653792 12972531
|
|
|
|
| BHK21 CCL-10 hamster fibroblast adherent hamster, Syrian golden kidney, clone-13
|
|
|
|
| from 19276070(M): MRE-beta-Geo reporter cell line, BHK3038, was kindly provided by Dr. R. D. Palmiter (University of Washington, Seattle, WA).
|
|
|
|
| normal human skin fibroblasts
|
|
|
|
| Burkitt lymphoma, human 10692572(C)
|
|
|
|
| B-cell lymphoma 12150925(C) 12023955(C)
|
|
|
|
| human EBV-negative Burkitt lymphoma B cell line 9133417(C)
|
|
|
|
| BJT cells were derived from normal human BJ fibroblasts that were immortalized by ectopic expression of the telomerase enzyme. 12654245(C)
|
|
|
|
| Epstein-Barr virus-negative, sIgM+ human B cell lines 14978013(C)
|
|
|
|
| a Burkitt's lymphoma cell line 3136546(C)
|
|
|
|
| express barely detectable mRNA for IL6R 3136546-Fig-3
|
|
|
|
| contain no detectable IL6 binding sites 3136546-Fig-3
|
|
|
|
| Epstein-Barr virus-negative, sIgM+ human B cell lines 14978013(C)
|
|
|
|
| a hepatoma line 2261637(C)
|
|
|
|
| expresses mRNA for IL6R and GP130 2261637-Fig-4
|
|
|
|
| human cell line derived from Hek293 [28] 9020886(C)
|
|
|
|
| 28 Pear, W. S., Nolan, G. P., Scott, M. L. and Baltimore, D. (1993) Proc. Natl. Acad.
Sci. U.S.A. 90, 8392–8396
|
|
|
|
| BPH-1 is a benign prostatic hyperplasia epithelial cell line which expresses PTK6 (23). BPH-1 cells also express high levels of EGF receptor, and EGF is able to induce strong tyrosine phosphorylation in this cell line (17). 20606012(C)
|
|
|
|
| 23. Hayward, S. W., R. Dahiya, G. R. Cunha, J. Bartek, N. Deshpande, and P. Narayan. 1995. Establishment and characterization of an immortalized but non-transformed human prostate epithelial cell line: BPH-1. In Vitro Cell. Dev. Biol. Anim. 31:14–24.
|
|
|
|
| 17. El Sheikh, S. S., J. Domin, P. Abel, G. Stamp, and E.-N. Lalani. 2004. Phosphorylation of both EGFR and ErbB2 is a reliable predictor of prostate cancer cell proliferation in response to EGF. Neoplasia 6:846–853.
|
|
|
|
| a murine hepatoma line that lacks ARNT/HIF-1b, and thus, functional HIF-1 complex (41, 42)
|
|
|
|
| 41. Miller, A. G., and Whitlock, J. P., Jr. (1981) J. Biol. Chem. 256, 2433–2437
|
|
|
|
| 42. Miller, A. G., Israel, D., and Whitlock, J. P., Jr. (1983) J. Biol. Chem. 258,
3523–3527
|
|
|
|
| "buffalo rat liver cells" 11598209(C)
|
|
|
|
| maybe BRL 3A CRL-1442 rat liver adherent
|
|
|
|
| derived from BHK cells and constitutively expresses the T7 polymerase
|
|
|
|
| provided by Karl-Klaus Conzelmann, Max Pettenkofer Institut
|
|
|
|
| expresses Huwe1 protein 15989956(D)
|
|
|
|
|
epithelial, mammary gland; breast, duct, ductal carcinoma
|
|
|
|
|
| adherent, patchy (The cells form adherent patches of epithelial-like cells The patches are compact multilayered colonies that rarely become confluent)
|
|
|
|
| The BT-474 line was isolated by E. Lasfargues and W.G. Coutinho from a solid, invasive ductal carcinoma of the breast.
|
|
|
|
| does not express Huwe1 protein 15989956(D)
|
|
|
|
| derived from ER-positive breast cancer tissues 16596714(C)
|
|
|
|
| overexpress EgfR and Erbb3 16596714(C)
|
|
|
|
| expresses low levels of Huwe1 protein 15989956(D)
|
|
|
|
| BT-549 HTB-122 human epithelial adherent mammary gland; ductal carcinoma
|
|
|
|
| does not express Huwe1 protein 15989956(D)
|
|
|
|
| BxPC-3 CRL-1687 human pancreas; adenocarcinoma adherent
|
|
|
|
| Smad4-null 17638924(D) [WB]
|
|
|
|
| CDKN2A p.M1_*157del c.1_471del471 Homozygous
MAP2K4 p.? c.1041_1200del160 Homozygous
SMAD4 p.? c.1_1659del1659 Homozygous
TP53 p.Y220C c.659A>G Homozygous
|
|
|
|
| mouse Ink4a/Arf null melanocyte C140 cells 19187764(C)
|
|
|
|
| murine myoblast 16806820(C)
|
|
|
|
| human Lymphoblasts from a normal donor 9168117(C)
|
|
|
|
| human Lymphoblasts from a normal donor 9168117(C) 14532133(C)
|
|
|
|
| lymphoblasts expressing WT Atm 15073328(C)
|
|
|
|
| C2C12 CRL-1772 mouse fibroblast adherent muscle
|
|
|
|
| used in 11265759 without explanation
|
|
|
|
| "mouse mygenic cells", "skeletal muscle cells" 14525954(C)
|
|
|
|
| can be differentiated into "myotubes" by culturing confluent cells for 4 days in DMEM in 2% horse serum
|
|
|
|
| endogenously expresses PpaRd(C)
|
|
|
|
| 16945942(C) mouse C2C12 satellite cells
|
|
|
|
| differentiated by switching cells from 10% FBS to 1% HS for 7 days
|
|
|
|
| C30.1: a cytotoxic T cell line provided by Dr F Denizot 1092780
|
|
|
|
| In the absence of cytokines, C30.1 cells become resting and can be subsequently activated either by IL-2 or by IL-4 (21).
|
|
|
|
| 21. Chastagner, P., J.-L. Moreau, Y. Jacques, T. Tanaka, M. Miyasaka, M. Kondo, K. Sugamura, and J. The`ze. 1996. Lack of intermediate-affinity interleukin-2 receptor in mice leads to independence on interleukin-2 receptor ��, �� and �� chain expression for T cell growth. Eur. J. Immunol. 26:201.
|
|
|
|
| The retinoblastoma protein (pRB) is present but abnormal in size 1648218(D)
|
|
|
|
| C33-A cervical carciinoma human adherent
|
|
|
|
| express high levels of a mutant p53 protein that is still sensitive to degradation by Mdm2 9153396(C)
|
|
|
|
| Wrede D et al Mol Carcinog 4,171=175 (1991)
|
|
|
|
| Rb1 negative 10499802(C) no data or refs provided
|
|
|
|
| C3H/10T1/2 CCL-226 mouse fibroblast adherent embryo Clone 8
|
|
|
|
| C3H10T12 cells transfected with Hras(G12V) are called 11A cells in 11358964
|
|
|
|
| C3H10T12 cells expresses Pkca, Pkcd, Pkce, Pkch but not Pkcb or Pkcg 11358964(D)
|
|
|
|
| a mammary epithelial cell line 10421629(C)
|
|
|
|
| Caco2 HTB-37 human epithelial adherent colon; colorectal adenocarcinoma
|
|
|
|
| Caki-1 HTB-46 human skin (metastasis); clear cell carcinoma (kidney primary) epithelial adherent
|
|
|
|
| Calu3 HTB-55 human pleural effusion (metastasis); adenocarcinoma (lung primary) epithelial adherent
|
|
|
|
| expresses high levels of Huwe1 protein 15989956(D)
|
|
|
|
| Capan-1 HTB-79 human liver (metastasis); adenocarcinoma (pancreas primary) epithelial adherent
|
|
|
|
| Smad4-null 17638924(D) [WB]
|
|
|
|
| BRCA2 p.S1982fs*22 c.5946delT Homozygous
CDKN2A p.M1_*157del c.1_471del471 Homozygous
KRAS p.G12V c.35G>T Homozygous
SMAD4 p.S343* c.1028C>G Homozygous
TP53 p.A159V c.476C>T Homozygous
|
|
|
|
| express Brca1 protein 10550055-Fig-1c
|
|
|
|
| a previously characterized DC line 9768751(C)
|
|
|
|
| Paglia, P., Girolomoni, G., Robbiati, F., Granucci, F., and Ricciardi- Castagnoli, P. (1993). Immortalized dendritic cell line fully competent in antigen presentation initiates primary T cell responses in vivo. J. Exp. Med. 178, 1893–1901.
|
|
|
|
| express IL12Rb1 mRNA 9768751-Fig-3
|
|
|
|
| express IL12Rb2 mRNA 9768751-Fig-3
|
|
|
|
| carbonyl cyanide 3-chlorophenylhydrazone
|
|
|
|
| mitochondrial uncoupler 14709557(C)
|
|
|
|
| used in 15258589 with out explanation
|
|
|
|
| CCD-18Co CRL-1459 human colon fibroblast adherent
|
|
|
|
| ChangLiver CCL-13 human epithelial adherent HeLa contaminant
|
|
|
|
| used without explanation in 9774335 9733512
|
|
|
|
| possibly: Dede ATCC CCL-39 hamster fibroblast adherent lung
|
|
|
|
| Chinese hamster lung fibroblast cell line 11864996 11154281
|
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|
|
| ATCC CCL-119 CCRF-CEM [CCRF CEM]
|
|
|
|
|
suspension Homo sapiens (human) lymphoblast
|
|
|
Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: T lymphoblast
|
|
|
|
|
| CDKN2A p.0? c.1_471del471 Homozygous Verified
CDKN2a(p14) p.? c.317_522del206 Homozygous Verified
FBXW7 p.R465C c.1393C>T Heterozygous
FLT3 p.A627T c.1879G>A Heterozygous
KRAS p.G12D c.35G>A Heterozygous Verified
MLH1 p.R100* c.298C>T Heterozygous Verified
MLH1 p.? c.790+1G>A Heterozygous Verified
NOTCH1 p.R1595>PRLPHNSSFHFLR c.4783_4784ins36 Heterozygous
PTEN p.? c.80_492del413 Homozygous Verified
TP53 p.R175H c.524G>A Heterozygous Verified
TP53 p.R248Q c.743G>A Heterozygous Verified
|
|
|
|
| expresses mRNA for Gp130 but not IL6R 2261637-Fig-4
|
|
|
|
| CESS TIB-190 human lymphoblast suspension lymphoblast; myelomonocytic leukemia
|
|
|
|
| expresses mRNA for IL6R 3136546-Fig-3
|
|
|
|
| contain IL6 binding sites 3136546-Fig-3
|
|
|
|
| The mouse B cell line CH12.LX expresses surface IgM specific for phosphatidylcholine, an Ag found on the surface of SRBC (11), and has been described previously (12) 12133933(C)
|
|
|
|
| 11. Mercolino, T. J., L. W. Arnold, and G. Haughton. 1986. Phosphatidyl choline is recognized by a series of Ly-1-pos murine B cell lymphomas specific for erythro- cyte membranes. J. Exp. Med. 163:155.
|
|
|
|
| 12. Bishop, G. A., and G. Haughton. 1986. Induced differentiation of a transformed clone of Ly-1-pos B cells by clonal T cells and antigen. Proc. Natl. Acad. Sci. USA 83:7410.
|
|
|
|
| CH12.LX is diploid or near-diploid (karyotype analysis performed by Dr. Baoli Yang, University of Iowa). 12958312(C)
|
|
|
|
| CH12T2-\-: CH12LX<Traf2-null> 12958312(D)
|
|
|
|
| ChangLiver CCL-13 human epithelial adherent HeLa contaminant
|
|
|
|
| This line was originally thought to be derived from normal liver tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination.
|
|
|
|
| Chinese Hamster Ovary Cells - see CHOK1
|
|
|
|
| used without explanation in 11598209
|
|
|
|
| Chinese hamster ovary (CHO)–derived A03.1 cells contain an integrated array of ~10,000 lac operator (lacO) sites at a single chromosome locus that was generated by coamplification with a dihydrofolate reductase (DHFR) expression cassette (46). 19417216(C)
|
|
|
|
| 46. Tumbar T, Sudlow G, Belmont AS. Large-scale chromatin unfolding and remodeling induced by VP16 acidic activation domain. J Cell Biol 1999;145:1341–1354. [PubMed: 10385516]
|
|
|
|
| used without explanation in 10567225
|
|
|
|
| CHO-K1 CCL-61 hamster epithelial adherent ovary
CHO-K1 CRL-9618 hamster epithelial adherent ovary
|
|
|
|
| A methotrexate-resistant CHO cell strain 8832394(C)
|
|
|
|
| Milbrandt, J. D., Heintz, N. H., White, W. C. Rothman, S. M. and Hamlin,
J. L. (1981). Methotrexate-resistant Chinese hamster ovary cells have amplified a 135-kilobase-pair region that includes the gene for dihydrofolate reductase. Proc. Nat. Acad. Sci. USA 78, 6042-6047.
|
|
|
|
| human monoclonal IgM-pos IgD-pos cell line 10228008(C)
|
|
|
|
| 13. Cerutti, A., H. Zan, A. Schaffer, L. Bergsagel, N. Harindranath, E. E. Max, and P. Casali. 1998. CD40 ligand and appropriate cytokines induce switching to IgG, IgA, and IgE and coordinated germinal center and plasmacytoid phenotypic dif- ferentiation in a human monoclonal IgM-pos IgD-pos B cell line. J. Immunol. 160: 2145.
|
|
|
|
| 14. Cerutti, A., A. Schaffer, S. Shah, H. Zan, H.-C. Liou, R. G. Goodwin, and P. Casali. 1998. CD30 is a CD40-inducible molecule that negatively regulates CD40-mediated immunoglobulin class switching in non antigen-selected human B cells. Immunity 9:247.
|
|
|
|
| 15. Zan, H., A. Cerutti, P. Dramitinos, A. Schaffer, and P. Casali. 1998. CD40 en- gagement triggers switching to IgA1 and IgA2 in human B cells through induc- tion of endogenous TGF-b1: evidence for TGF-b but not IL-10-dependent direct Smu-Salpha and sequential Smu-Sgamma, Sgamma-Salpha DNA recombination. J. Immunol. 161:5217.
|
|
|
|
| a model megakaryocytic cell line (24) 9099734(C)
|
|
|
|
| The CMK cell line, provided by T. Sato (Chiba Univer- sity, Japan), was maintained in RPMI 1640 medium with 10% fetal calf serum as described previously (31). The CMK cell line has properties of cells of the megakaryocytic lineage because it can proliferate in response to cytokines and differentiate upon induction with PMA (24, 32).
|
|
|
|
| 24. Sakaguchi, M., Sato, T., and Groopman, J. E. (1991) Blood 77, 481–485
|
|
|
|
| 31. Sato, T., Fuse, A., Eguchi, M., Hayashi, H., Ryo, R., Adachi, M., Kishimoto, Y., Teramura, M., Mizoguchi, H., Shima, Y., Komon, I., Sunami, S., Okiomoto, Y., and Nakajima, H. (1989) Br. J. Haematol. 72, 184–190
|
|
|
|
| 32. Cowley,S.A.,Groopman,J.E.,andAvraham,H.(1992)Int.J.CellCloning10, 223–231
|
|
|
|
| CMT-93 CCL-223 mouse epithelial adherent rectum; polyploid carcinoma
|
|
|
|
| COLO 205 CCL-222 human ascites (metastasis); colorectal adenocarcinoma epithelial mixed (colon primary)
|
|
|
|
| colon carcinoma 10197981(C)
|
|
|
|
| APC p.T1556fs*3 c.4666_4667insA Homozygous
BRAF p.V600E c.1799T>A Heterozygous
SMAD4 p.? c.1_904del904 Homozygous
TP53 p.Y103_L111>L c.308_333>TA Homozygous
|
|
|
|
| COLO 320DM CCL-220 human rounded, mixed refractile colon; colorectal adenocarcinoma
|
|
|
|
| COLO 320HSR CCL-220.1 human rounded, mixed refractile colon; colorectal adenocarcinoma
|
|
|
|
| does not expresses Stat6 mRNA [Northern] 7760829-Fig-2
|
|
|
|
| expresses WT myc (33) 14563837(C)
|
|
|
|
| 33. Rabbitts, T. H., Hamlyn, P. H., and Baer, R. (1983) Nature 306, 760–765
|
|
|
|
| human pancreatic cancer cell line 21573184(C)
|
|
|
|
| 16. Jaganathan S, Yue P, Turkson J (2010) Enhanced sensitivity of pancreatic cancercells to concurrent inhibition of aberrant signal transducer and activator oftranscription 3 and epidermal growth factor receptor or Src. J Pharmacol ExpTher 333: 373–381.
|
|
|
|
| 17. Turkson J, Ryan D, Kim JS, Zhang Y, Chen Z, et al. (2001) Phosphotyrosylpeptides block Stat3-mediated DNA-binding activity, gene regulation and celltransformation. J Biol Chem 276: 45443–45455.
|
|
|
|
| used in many refs without explanation - possibly Cos1 or Cos7
|
|
|
|
| COS-1 CRL-1650 African green moneky kidney fibroblast adherent
|
|
|
|
| also used when cells are only described as "COS" cells, as in 9416833(C)
|
|
|
|
| COS-1 cells do not contain sufficient amounts of endogenous isoprenoid precursors to farnesylate overexpressed Ras proteins, so medium was supplemented with mevalonolactone to augment available precursors. 10358073(C)
|
|
|
|
| 11686307(D) COS cells produce only MHC-B [Myh10] not MHC-A [Myh9]
|
|
|
|
| COS-7 CRL-1651 African green moneky kidney fibroblast adherent
|
|
|
|
| 10620507 uses Cos7-M6 without explanation
|
|
|
|
| express ArhGef7 but not ArhGef6 9659915-Fig-4
|
|
|
|
| used in 8272873 without explanation
|
|
|
|
| used in 10652228 without explanation
|
|
|
|
| a murine T cell line the requires either IL2 or IL4 for growth 7991937(C)
|
|
|
|
| 18. Hu-Li, J., J. Ohara, C. Watson, W. Tsang, and W. E. Paul. 1989. Derivation of a T cell line that is highly responsive to IL-4 and IL-2 (CT.4R) and of an IL-2 hyporesponsive mutant of that line (CT.4S). J . lmmunol. 142:800.
|
|
|
|
| 8069916(C): murine IL2 dependent cytotoxic T cell line
|
|
|
|
| 8692855(C): murine IL2 dependent cell line
|
|
|
|
| a murine IL3 dependent mast cell line (C15) 10072514(C)
|
|
|
|
| 7. Pierce, J. H., P. P. DiFiore, S. A. Aaronson, M. Potter, J. Pumphrey, A. Scott, and J. N. Ihle. 1985. Neoplastic transformation of mast cells by Abelson-MuLV:
abrogation of IL-3 dependence by a nonautocrine mechanism. Cell 41:685.
|
|
|
|
| murine cell line used without explanation in 10654938
|
|
|
|
| CTLL-2 TIB-214 mouse lymphoblast suspension T lymphocyte; cytotoxic
|
|
|
|
| IL2 dependent cytolytic T-cell line [1996324(C)][19]
|
|
|
|
| 19. Gillis, S. & Smith, K. (1977) Nature (London) 268, 154-156.
|
|
|
|
| expresses surface IL2Ra [FACS] 3110787-Fig-3
|
|
|
|
| expresses surface IL2Rg [FACS] 8266076-Fig-1
|
|
|
|
| a IL2 dependent T cell line 8294403(C) 8643566(C)
|
|
|
|
| a murine IL2 dependent cell line 7528328(C)
|
|
|
|
| hematopoietic cell line established from a patient with acute myeloblastic leukemia 10753828(C)
|
|
|
|
| A hematopoietic progenitor cell line, CTS, was established from a patient with acute myeloblastic leukemia27 and has been previously used as a model of SDF-1a–mediated migration and signaling.(22,23). The CTS cell line was grown at 37°C in 5% CO2 in RPMI-1640 medium containing 10% fetal calf serum, 50 ug/mL penicillin, and 50 ug/mL streptomycin, as described previously. (22,23,27) 11369622(C)
|
|
|
|
| 22. Dutt P, Wang JF, Groopman JE. Stromal cell- derived factor-1 alpha and stem cell factor/kit li- gand share signaling pathways in hemopoietic progenitors: a potential mechanism for coopera- tive induction of chemotaxis. J Immunol. 1998; 161:3652-3658.
|
|
|
|
| 23. Wang JF, Park IW, Groopman JE. Stromal cell- derived factor-1a stimulates tyrosine phosphorylation of multiple focal adhesion proteins and induces migration of hematopoietic progenitor cells: roles of phosphoinositide-3 kinase and protein kinase C. Blood. 2000;95:2505-2513.
|
|
|
|
| 27. Kakuda H, Sato T, Hayashi Y, Enomoto Y, et al. A novel human leukaemic cell line, CTS, has a t(6;11) chromosomal translocation and character- istics of pluripotent stem cells. Br J Haematol. 1996;95:306-318.
|
|
|
|
| CV-1 CCL-70 monkey fibroblast adherent African Green monkey kidney
|
|
|
|
| "nonmuscle cells" "African green monkey cells" 14525954(C)
|
|
|
|
| T cells used in 11073940(C)
|
|
|
|
| a primary fibroblast cell line established from an ATLD patient homozygous for the R633X nonsense mutation (38) 16478990(C)
|
|
|
|
| 38. Stewart, G. S., R. S. Maser, T. Stankovic, D. A. Bressan, M. I. Kaplan, N. G. Jaspers, A. Raams, P. J. Byrd, J. H. Petrini, and A. M. Taylor. 1999. The DNA double-strand break repair gene hMRE11 is mutated in individuals with an ataxia-telangiectasia-like disorder. Cell 99:577–587.
|
|
|
|
| Mre11 prot-exp[WB] is undetectable 16478990-Fig-4a
|
|
|
|
| murine lymphoma 9153395(C)
|
|
|
|
| Tp53-null mouse fibroblasts 10806474(C)
|
|
|
|
| an IL3 dependent myeloid line 7760829(C)
|
|
|
|
| Daudi CCL-213 human lymphoblast suspension peripheral blood, B lymphoblast; Burkitt’s lymphoma
|
|
|
|
| lymphoblastoid cell line 9374471(C)
|
|
|
|
| expresses mRNA for Gp130 but not IL6R 2261637-Fig-4
|
|
|
|
| expresses WT myc (33) 14563837(C)
|
|
|
|
| 33. Rabbitts, T. H., Hamlyn, P. H., and Baer, R. (1983) Nature 306, 760–765
|
|
|
|
| a NK-like cell line 17110450(C)
|
|
|
|
| Di Noto R, Pane F, Camera A, et al. Characterization of two novel cell lines, DERL-2 (CD5-pos/ CD3-pos/Tcry-pos) and DERL-7 (CD5-pos/CD3-neg/ TCRgd-neg), derived from a single patient with CD56-neg non-Hodgkin’s lymphoma. Leukemia. 2001;15:1641-1649.
|
|
|
|
| dermatofibrosarcoma protuberans cell line
|
|
|
|
| contains constitutively active PdgfR 12177062(C)
|
|
|
|
| Epstein-Barr virus-transformed B cell line 7721786(C)
|
|
|
|
| a lymphoblastoid cell line derived from a Seckel-ATR patient, in which very low levels of full-length ATR are present due to a mutation effecting splicing of the transcript 16087684(C)
|
|
|
|
| 16. O’Driscoll, M., Ruiz-Perez, V.L., Woods, C.G., Jeggo, P.A. and Goodship, J.A. (2003) A splicing mutation affecting expression of ataxia- telangiectasia and Rad3-related protein (ATR) results in Seckel syndrome. Nat. Genet., 33, 497 – 501.
|
|
|
|
| a lymphoblastoid cell line derived from an unaffected sibling of a Seckel-ATR patient 16087684(C)
|
|
|
|
| 16. O’Driscoll, M., Ruiz-Perez, V.L., Woods, C.G., Jeggo, P.A. and Goodship, J.A. (2003) A splicing mutation affecting expression of ataxia- telangiectasia and Rad3-related protein (ATR) results in Seckel syndrome. Nat. Genet., 33, 497 – 501.
|
|
|
|
| DLD-1 CCL-221 human colon; colorectal adenocarcinoma epithelial adherent
|
|
|
|
| mutations: Kras(G13D)"CA" Tp53-null Apc-null 10197981(C)
|
|
|
|
| 16. M. van de Wetering, M. Oosterwegel, K. van Norren, H. Clevers, EMBO J. 12, 3847 (1993); V. Korinek and N. Barker, unpublished data.
|
|
|
|
| Murphy, K. M., A. B. Heimberger, and D. Y. Loh. 1990. Induction by antigen on intrathymic apoptosis of CD4-pos CD8-pos TCR-low thymocytes in vivo. Science 250:
1720.
|
|
|
|
| The p53-negative murine erythroleukemia cellline DP16-1(16,41) was derived from the spleen cells of a DBA/2J mouse infected with the polycythemia strain of Friend murine erythroleukemia virus complex.
|
|
|
|
| 16. Hicks, G., and M. Mowat. 1988. Integration of Friend murine leukemia virus into both alleles of the p53 oncogene in an erythroleukemic cell line. J. Virol. 62:4752-4755.
|
|
|
|
| 49. Yewdell,J.W.,J.V.Gannon,andD.P.Lane.1986.Monoclonal antibodyanalysisofp53expressioninnormal andtransformed cells.J.Virol.59:444 452.
|
|
|
|
| A Tgfb1-resistant mutant of MV1LU which lacks TgfbR2 1333888(C)
|
|
|
|
| The mkT6R-II receptor expressed in DR-26 cells contains a single base mutation (C to T) that transforms the seventh codon of the transmembrane region into a stop codon predicting a truncated receptor II protein that, if stable, is likely to be released into the medium. 1333888(D)
|
|
|
|
| A Tgfb1-resistant mutant of MV1LU which lacks TgfbR2 1333888(C)
|
|
|
|
| In DR-27 cells, receptor II contains a single base mutation (G to A) that converts the third cysteine in the extracellular domain to tyrosine (Figure 3) a change that may prevent TGFf3
binding owing to incorrect folding of the binding site. 1333888(D)
|
|
|
|
| used without explanation in 12771962
|
|
|
|
| used without explanation in 15268862
|
|
|
|
| DT40 CRL-2111 chicken lymphoblast suspension bursa; lymphoma
|
|
|
|
| DU 145 HTB-81 human epithelial adherent brain (metastasis); carcinoma (prostate primary)
|
|
|
|
| CDKN2A p.D84Y c.250G>T Homozygous
MLH1 p.? c.117-2A>T Homozygous
RB1 p.K715* c.2143A>T Homozygous
STK11 p.K178fs*86 c.532_536delAAGCC Homozygous
TP53 p.V274F c.820G>T Heterozygous
TP53 p.P223L c.668C>T Heterozygous
|
|
|
|
| do not express Huwe1 protein 15989956(D)
|
|
|
|
| The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. [PubMed: 6407019] [PubMed: 2079463]
|
|
|
|
| EA.hy926 used in 15793306:
|
|
|
|
| the EA.hy926 cell line, formed by fusion of HUVECs with the human lung carcinoma cell line A549, which retains EC-like responses to TNF, and, as reported here, displays an extensive caveolar system.
|
|
|
|
| The human-derived EA.hy926 EC line [24] (provided by Dr. W. Sessa, Yale University, New Haven, CT)
|
|
|
|
| 24. Edgell CJ, McDonald CC, Graham JB: Permanent cell line expressing
human factor VIII-related antigen established by hybridization. Proc
Natl Acad Sci USA 1983, 80:3734 –3737
|
|
|
|
| human, used without more explanation 14657239(C)
|
|
|
|
| A derivative of T24 human bladder line
|
|
|
|
| T24 HTB-4 human epithelial adherent urinary bladder; transitional cell carcinoma
|
|
|
|
| http://www.cell-lines-service.de/content/e143/e1751/e1204/index_eng.html
|
|
|
|
| ED40515(-): a cell line, IL2Ra+ IL2Rb+ IL2Rg- 9512511(C)
|
|
|
|
| Arima, N., M. Kamio, K. Imada, T. Hori, T. Hattori, M. Tsudo, M. Okuma, and T. Uchiyama. 1992. Pseudo-high affinity interleukin-2(IL-2) receptor lacks the third component that is essential for functional IL-2 binding and signaling. J. Exp. Med. 176: 1265–1272.
|
|
|
|
| HTLV1 treansformed human Cd4+ Cd8- T cell line which expresses IL2Ra IL2Rb but not IL2Rg and proliferates independently of IL2
|
|
|
|
| Maeda, M., Shimizu, A., Ikuta, K., et al. (1985) Origin of humanT-lymphotrophic virus I-positive T cell lines in adult T cellleukemia: analysis of T cell receptor gene rearrangement.J. Exp. Med. 162, 2169–2174.
|
|
|
|
| Arima, N., Kamio, M., Imada, K., et al. (1992) Pseudo-highaffinity interleukin 2 (IL-2) receptor lacks the third compo-nent that is essential for functional IL-2 binding and signalling.J. Exp. Med. 176, 1265–1272.
|
|
|
|
| Asao, H., Tanaka, N., Ishii, N., et al. (1994) Interleukin 2-induced activation of JAK3: possible involvement in signaltransduction for c-myc induction and cell proliferation. FEBSLett. 351, 201–206.
|
|
|
|
| an IL2 dependent leukemic T cell line 1940342(C)
|
|
|
|
| 36. Maeda, M.. A. Shimiiu, K. Ikuta, H. Okamoto, M. Kashihara, T. Uchiyama, T. Honjo. and J. Yodoi. 1985. Origin of human T-lymphotropicvirus I-positive T cell lines in adult T cellleukemia: analysis of T cell receptorgene rearrangement. J . Exp. Med. 162:2169.
|
|
|
|
| TSC2 positive cells derived from Eker rat embryos (8) 12167664
|
|
|
|
| 8. Aicher, L. D., Campbell, J. S., and Yeung, R. S. (2001) J. Biol. Chem. 276,
21017–21021
|
|
|
|
| called EEF126-4 in 17277771(C)
|
|
|
|
| fibroblasts, used without more explanaation in 14651849
|
|
|
|
| also called EEF126-8 (EEF8, TSC2 -/-)
|
|
|
|
| TSC2 negative cells derived from Eker rat embryos (8) 12167664(C)
|
|
|
|
| 8. Aicher, L. D., Campbell, J. S., and Yeung, R. S. (2001) J. Biol. Chem. 276,
21017–21021
|
|
|
|
| called EEF126-8 in 17277771(C)
|
|
|
|
| human epithelial bladder derived cell line 9020886(C) 16260598(C)
|
|
|
|
| EJ bladder carcinoma cells were obtained from the European Collection of Cell Cultures (Porton Down, U.K.).
|
|
|
|
| EL4 TIB-39 mouse lymphoblast suspension T lymphocyte; lymphoma
|
|
|
|
| mouse mammary epithelial cell line 10197981(C)
|
|
|
|
| The parental cell line EpH4 is nontumorigenic and responds to TGFb1 with growth inhibition, whereas its v-Ha-Ras-transformed derivative, EpRas, responds with a transition into a fibroblastoid phenotype that is not growth inhibited and is highly invasive in vivo (Oft et al. 1996).
|
|
|
|
| Oft, M., J. Peli, C. Rudaz, H. Schwarz, H. Beug, and E. Reichmann. 1996. TGF-beta-1 and Ha-Ras collaborate in modulating the phenotypic plasticity and invasiveness of epithelial tu-
mor cells. Genes & Dev. 10: 2462–2477.
|
|
|
|
| CCL39 cells expressing EgfR 9733714
|
|
|
|
| 17. Sardet, C., Fafournoux, P., and Pouyssegur, J. (1991) J. Biol. Chem. 266,
19166 –19171
|
|
|
|
| cell line derived from Eker rats - lacks functional Tsc2 9809973(C)
|
|
|
|
| used without explanation in 8272873
|
|
|
|
| a Rad50 deficient cell line 21149446(C)
|
|
|
|
| expresses normal levels of Atm protein but greatly reduced levels of Mre11 Rad50 Nbs1 21149446-Fig-6c
|
|
|
|
| NF2(Merlin)-pos, p53-null osteosarcoma cells 16535381(C)
|
|
|
|
| 23. McClatchey, A. I., Saotome, I., Mercer, K., Crowley, D., Gusella, J. F., Bronson, R. T. & Jacks, T. (1998) Genes Dev. 12, 1121–1133.
|
|
|
|
| F9 CRL-1720 mouse epithelial adherent testis; embryonic carcinoma; testicular teratoma
|
|
|
|
| lack Jun and Fos [15,16] 9732876 (C)
|
|
|
|
| 5. Chiu, R. etal. The c-Fos protein interacts with c-Jun/AP-1to stimulate transcription of AP-1 responsive genes. Cell 54, 541–552(1988).
6. Yang-Yen, H.F., Chiu, R. &Karin,M. Elevation of AP-1activity during F9 cell differentiation is due to increased c-Jun transcription. NewBiol. 2, 351–361(1990).
|
|
|
|
| lack detectable levels of Jun 16511568
|
|
|
|
| Yang-Yen HF, Chiu R, Karin M (1990) Elevation of AP1 activity during F9 cell differentiation is due to increased c-jun transcription. New Biologist 2: 351–361
|
|
|
|
| van Dam H, Wilhelm D, Herr I, Steffen A, Herrlich P, Angel P (1995) ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents. EMBO J 14: 1798–1811
|
|
|
|
| RGS mRNA[RTPCR] expression 17855383(D)
|
|
|
|
| Rgs1-neg
Rgs2-neg
Rgs3-pos
Rgs4-neg
Rgs5-low
Rgs13-neg
Rgs16-pos
Rgs18-neg
Rgs10-pos
Rgs12-pos
Rgs14-pos
Rgs20-pos
Rgs17-pos
Rgs19-pos
Axin1-pos
Axin2-pos
|
|
|
|
| rat hepatoma 10688912(C) 15069087(C)
|
|
|
|
| murine myeloid cell line 8995447(C)
|
|
|
|
| IL-4-dependent myeloid cell
|
|
|
|
| 14. Welham, M. J., Learmonth, L., Bone, H., and Schrader, J. W. (1995) J. Biol. Chem. 270, 12286 –12296
|
|
|
|
| FDC-P1 CRL-12103 mouse lymphoblast suspension bone marrow
|
|
|
|
| 10910062(C): murine IL-3-dependent myelomonocytic progenitor cell line
|
|
|
|
| 8124718(C): an IL3 dependent cell line
|
|
|
|
| 10654938(C): express Csf3 receptors
|
|
|
|
| a subclone of FDCP1 produced by continuous culturing in Csf1 which expresses the endogenous Csf1R and can grow in Csf1 as the sole hematopeietic growth factor 1710692(D)
|
|
|
|
| A multipotent factor-dependent cell line, FDCP-mix. These cells possess a nonleukemic phenotype, have a normal karyotype, are factor dependent, and can be directed to undergo multilineage development in response to cytokines (77). By changing growth conditions (serum and cytokines), it is possible to grow this cell line under self-renewing conditions, such that they retain their multipotent state; alternatively, they may be directed to differentiate to form erythroid or myeloid cells. 16648489(C)
|
|
|
|
| mouse mammary tumor cell line 9003781(C)
|
|
|
|
| FRTL CRL-1468 rat thyroid adherent
|
|
|
|
| human fibroblasts 1496401(C)
|
|
|
|
| FS-4 cells derived from human foreskin fibroblasts were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal calf serum 11352916(C)
|
|
|
|
| ATCC CRL-1424 Disease: malignant melanoma Organ: skin epithelial
|
|
|
|
| Continuous culture, grown as monolayer
epithelial morphology
SPECIES: human, Caucasian (male, 31 years old) Karyology: subtetraploid
TISSUE/ORGAN: skin
TUMOR: malignant melanoma
tumorigenic in nude mice
PROPERTIES: melanin prod.; cell biology; tumorigenicity; hormone studies
|
|
|
|
| BRAF p.V600E c.1799T>A Heterozygous
CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.? c.317_522del206 Homozygous
STK11 p.P281fs*6 c.842delC Homozygous
|
|
|
|
| a somatic mutant derivative of the HT1080 human fibrosarcoma cell line selected after treatment with ICR191 for non-response to Ifng 7901766(M)
|
|
|
|
| a mutant derived from 2C4 cells 7537214(C)
|
|
|
|
| a mutant derived from 2C4 cells that lacks Jak2 mRNA and protein 7537214(C)
|
|
|
|
| does not express Jak2 protein 7537214-Fig-2a
|
|
|
|
| expresses Tyk2 protein 7537214-Fig-2a
|
|
|
|
| expresses Jak1 protein 7537214-Fig-2b
|
|
|
|
| Normal Epstein-Barr immortalized human lymphoblasts 9733515(C)
|
|
|
|
| SV40-transformed human skin fibroblasts from a healthy patient
|
|
|
|
| Epstein-Barr immortalized human lymphoblasts from a patient with AT
|
|
|
|
| Epstein-Barr immortalized human lymphoblasts from a patient with AT
|
|
|
|
| Untransformed fibroblasts from a patient with AT
|
|
|
|
| Untransformed fibroblasts from a patient with AT
|
|
|
|
| SV40-immortalized human fibroblast cell line, normal human 18990686(C)
|
|
|
|
| Epstein-Barr immortalized human lymphoblasts from a healthy patient
|
|
|
|
| Epstein-Barr immortalized human lymphoblasts from a patient with AT
|
|
|
|
| Epstein-Barr immortalized human lymphoblasts from a patient with AT
|
|
|
|
| Epstein-Barr immortalized human lymphoblasts from a patient with AT
|
|
|
|
| Untransformed fibroblasts from a clinically unaffected sister of an AT patient
|
|
|
|
| SV40-immortalized human skin fibroblast cell line from a patient with AT
|
|
|
|
| Epstein-Barr immortalized human lymphoblasts from a patient with Nijmegen breakage syndrome
|
|
|
|
| http://ccr.coriell.org/Sections/Search/Sample_Detail.aspx?Ref=GM07078&PgId=166
|
|
|
|
| Untransformed fibroblasts from a patien with Nijmegen breakage syndrome
|
|
|
|
| Untransformed fibroblasts from a patient with AT
|
|
|
|
| GM8523 fibroblasts immortalized with hTERT
|
|
|
|
| SV40-immortalized human skin fibroblast cell line from a patient with AT
|
|
|
|
| Chinese hamster/human hybrid retaining human chromosome #12
|
|
|
|
| Untransformed fibroblasts from a patient with Seckel Syndrome
|
|
|
|
| insulin responsive Chinese hamster ovary-derived cell line 16049009(C)
|
|
|
|
| used in heterkaryon assay in 12191473 without explanation
|
|
|
|
| The GTL-16 cell line is a clonal cell line derived from a poorly differentiated gastric carcinoma cell line(40). 2005882(C)
|
|
|
|
| 40. Motoyama, T., H. Hojo, T. Suzuki, and S. Oboshi. 1984. Evaluationoftheregrowthassay methodas an invitrodrug sensitivity test and its application to cultured human gastric cancer cellines.ActaMed.Biol.(NiigataUniversity,Niigata, Japan)27:49-52.
|
|
|
|
| the gastric carcinoma cell line GTL-16, which expresses approximately 100,000 receptors/cell (Giordano et al., 1989). In this cell line, overexpression causes constitutive phos- phorylation of the receptor (Ponzetto et al., 1991). 7513258(C)
|
|
|
|
| Giordano, S., Ponzetto, C., Di Renzo, M. F., Cooper, C. S., and Comog-
lio, P. M. (1969). Tyrosine kinase receptor indistinguishable from the c-Met protein. Nature 339, 155-l56.
|
|
|
|
| Ponzetto, C., Giordano, S., Peverali, F., Della Valle, G., Abate, M., Vaula, G., and Comoglio, P. M. (1991). c-met is amplified but not mu- tated in a cell line with an activated Met tyrosine kinase. Oncogene 6,553-559.
|
|
|
|
| human non small cell carcinoma aka NCI-H1299, NCIH1299
|
|
|
|
| NRAS p.Q61K c.181C>A Heterozygous
|
|
|
|
| p53-negative [RTPCR] 16676006-Fig-3i
|
|
|
|
| lung cancer cell line 16676006(C)
|
|
|
|
| p53-null human lung carcinoma 9153395(C)
|
|
|
|
| pRb-pos p53-neg 9724636(C)
|
|
|
|
| cell line deprived from lung cancer 11459832(C)
|
|
|
|
| H125, carries normal Rb. 11459832(C)
|
|
|
|
| H125 carries mutated p53. 11459832(C)
|
|
|
|
| immortalized H19-7 cells were generated from embryonic rat hippocampal cells as previously described (10). 10330161(C)
|
|
|
|
| 10. Eves, E. M., M. S. Tucker, J. D. Roback, M. Downen, M. R. Rosner, and
B. H. Wainer. 1992. Immortal rat hippocampal cell lines exhibit neuronal and glial lineages and neurotrophin gene expression. Proc. Natl. Acad. Sci. USA 89:4373–4377.
|
|
|
|
| human lung cancer cells from John Yannelli (Department of Microbiology, Immunology and Molecular Genetics, University of Kentucky, Lexington, KY). 17974977(C)
|
|
|
|
| mutant Kras status 17974977(C)
|
|
|
|
| Rat hepatoma cells (clone T-7-18 [4]) 10409724(C)
|
|
|
|
| 4. Baumann, H., K. R. Prowse, S. Marinokovic, K.-A. Won, and G. P. Jahreis. 1989. Stimulation of hepatic acute phase response by cytokines and glucocorticoids. Ann. N. Y. Acad. Sci. 557:280–295.
|
|
|
|
| human lung adenocarcinoma aka NCI-H358, NCIH358
|
|
|
|
| KRAS p.G12C c.34G>T Heterozygous
|
|
|
|
| H358 is P53-null. 11459832(C)
|
|
|
|
| H358 carries normal Rb. 11459832(C)
|
|
|
|
| human lung adenocarcinoma aka NCI-H460, NCIH460
|
|
|
|
| CDKN2A p.? c.1_457del457 Homozygous
|
|
|
|
| CDKN2a(p14) p.0? c.1_522del522 Homozygous
|
|
|
|
| KRAS p.Q61H c.183A>T Homozygous
|
|
|
|
| PIK3CA p.E545K c.1633G>A Heterozygous
|
|
|
|
| STK11 p.Q37* c.109C>T Homozygous
|
|
|
|
| H460 carries wild-type p53. 11459832(C)
|
|
|
|
| H460 carries normal Rb. 11459832(C)
|
|
|
|
| lung carcinoma cell line 18215320(C)
|
|
|
|
| mRNA: Fzd1-low Fzd2-pos Fzd3-neg Fzd4-neg Fzd5-low Fzd6-neg Fzd7-neg Fzd8-neg Fzd9-neg Fzd10-neg 18215320-Fig-2
|
|
|
|
| mRNA: Ror1-pos Ror2-neg 18215320-Fig-2
|
|
|
|
| mRNA: Lrp5-pos Lrp-6-pos 18215320-Fig-2
|
|
|
|
| rat hepatoma cell line 12524439(C)
|
|
|
|
| possibly ATCC CRL1600 H4-II-E-C3
|
|
|
|
| rat liver hepatoma adherent epithelial
|
|
|
|
| cell line deprived from lung cancer 11459832(C)
|
|
|
|
| H520 carries mutated p53. 11459832(C)
|
|
|
|
| human lung adenosquamous carcinoma aka NCI-H596 NCIH596
|
|
|
|
| H596 carries mutated p53 11459832(C)
|
|
|
|
| H596 has no Rb protein expression. 11459832(C)
|
|
|
|
| Bornschein et al. J. Cancer Mol. 4(2): 47-54, 2008
|
|
|
|
| atypical lung carcinoid cell line cultured as floating aggregates
|
|
|
|
| Bornschein et al. J. Cancer Mol. 4(2): 47-54, 2008
|
|
|
|
| typical bronchopulmonary carcinoid
|
|
|
|
| NCI-H838 CRL-5844 human lymph node (metastasis); adenocarcinoma; non-adherent small cell lung cancer (lung primary)
|
|
|
|
| CDKN2A p.M1_*157del c.1_471del471 Homozygous
STK11 p.T212fs*75 c.633delG Homozygous
TP53 p.E62* c.184G>T Homozygous
|
|
|
|
| H9 HTB-176 human lymphoblast suspension peripheral blood,T lymphocyte; cutaneous; lymphoma
|
|
|
|
| does not bind IL13 7721786-Table-1
|
|
|
|
| does not express Stat6 [Northern] 7760829-Fig-2
|
|
|
|
| Lymphoblastoid cell line from a Rad50 deficient patient 16858402(C)
|
|
|
|
| HaCat cells are a spontaneously immortalized, nontumorigenic keratinocyte cell line widely utilized as a model system for the study of structural and regulatory aspects of epithelial cell physiology and pathology (12, 13). 10969083(C)
|
|
|
|
| 12. Boukamp, P., Petrussevska, R. T., Breitkreutz, D., Hornung, J., Markham, A., and Fusenig, N. E. (1988) J. Cell Biol. 106, 761–771
13. Breitkreutz, D., Schoop, V. M., Mirancea, N., Baur, M., Stark, H. J., and Fusenig, N. E. (1998) Eur. J. Cell Biol. 75, 273–286
|
|
|
|
| expresses high levels of Pten mRNA 9187108(D)
|
|
|
|
| used without explanation in 16630891
|
|
|
|
| mouse mammary epithelial cells
|
|
|
|
| HC11 mouse mammary epithelial cells have been widely used as an in vitro model of mammary gland epithelial cell differentiation. The HC11 cell line preserves important features of mammary epithelial cell lactogenic differentia- tion; it was clonally derived from the COMMA-1D cells, a line immortalized from mammary tissue of a pregnant BALB/c mouse [1,2]. The HC11 cells are non-tumorigenic, display a normal epithelial phenotype, and the injection of HC11 cells into the cleared fat pad of BALB/c mice exhibited normal ductal and alveolar-like structures [1,3]. HC11 mammary epithelial cell lactogenic differentiation can be initiated in culture following the growth to conflu- ence and deposition of extracellular matrix in the presence of epidermal growth factor (EGF), subsequent removal of EGF from the culture and the addition of lactogenic hormone mix, DIP (dexamethasone, insulin, and prolactin); upon differentiation HC11 cells express specific milk pro- teins including β-casein [1]. Moreover, during lactogenic differentiation in culture the HC11 cells undergo pheno- typic transformation to "mammospheres", enlarged domed structures with a lumen [4-6].
|
|
|
|
| 74. Taverna D, Groner B, Hynes NE: Epidermal growth factor receptor, platelet-derived growth factor receptor, and c-erbB-2 receptor activation all promote growth but have distinctive effects upon mouse mammary epithelial cell differentiation. Cell Growth Differ 1991, 2(3):145-154.
|
|
|
|
| 9. Hynes NE, Taverna D, Harwerth IM, Ciardiello F, Salomon DS, Yamamoto T, Groner B: Epidermal growth factor receptor, but not c-erbB-2, activation prevents lactogenic hormone induction of the beta-casein gene in mouse mammary epithelial cells. Mol Cell Biol 1990, 10(8):4027-4034.
|
|
|
|
| colon cancer cell line expressing WT Apc 10092233(C)
|
|
|
|
| expresses Huwe1 protein 15989956(D)
|
|
|
|
| expresses Huwe1 protein 15989956(D)
|
|
|
|
| expresses Huwe1 protein 15989956(D)
|
|
|
|
| expresses Huwe1 protein 15989956(D)
|
|
|
|
| expresses Huwe1 protein 15989956(D)
|
|
|
|
| expresses Huwe1 protein 15989956(D)
|
|
|
|
| expresses Huwe1 protein 15989956(D)
|
|
|
|
| BRCA1 p.Q1756fs*74 (Insertion - Frameshift) c.5266_5267insC Homozygous
|
|
|
|
| TP53 p.R306* (Substitution - Nonsense) c.916C>T Homozygous
|
|
|
|
| express high levels of Pin1 11432833-Fig-2d
|
|
|
|
| do not express Brca1 protein 10550055-Fig-1c
|
|
|
|
| The HCE cell line was kindly provided by Roger Beuerman (LSU Eye Center, New Orleans, LA) and maintained as previously described (6). 15452053 (C)
|
|
|
|
| 6. Sharma GD, He J, Bazan HEP. p38 and ERK1/2 coordinate cellular migration and proliferation in epithelial wound healing: evidence of cross-talk activation between MAP kinase cascade. J Biol Chem. 2003;278:21989–21997.
|
|
|
|
| Human proximal tubular epithelial HKC cells (37) were obtained from Dr. L. Racusen of Johns Hopkins University and were cultured in DMEM-F-12 medium supplemented with 5% FBS. 10516287(C)
|
|
|
|
| 37. Racusen, L. C., C. Monteil, A. Sgrignoli, M. Lucskay, S. Marouillat, J. G. Rhim, and J. P. Morin. Cell lines with extended in vitro growth potential from human renal proximal tubule: characterization, response to inducers, and comparison with established cell lines. J. Lab. Clin. Med. 129: 318–329, 1997.
|
|
|
|
| human colorectal adenocarcinoma aka HCT-15
|
|
|
|
| APC p.I1417fs*2 c.4248delC Homozygous
APC p.R2166* c.6496C>T Heterozygous
|
|
|
|
| BRCA2 p.C1200fs*1 c.3599_3600delGT Homozygous
BRCA2 p.N1784fs*7 c.5351delA Heterozygous
|
|
|
|
| FAM123B p.E582* c.1744G>T Homozygous
|
|
|
|
| KRAS p.G13D c.38G>A Heterozygous
|
|
|
|
| MSH6 p.L290fs*1 c.868delC Heterozygous
MSH6 p.D1171fs*4 c.3511_3516>T Heterozygous
|
|
|
|
| PIK3CA p.E545K c.1633G>A Heterozygous
PIK3CA p.D549N c.1645G>A Heterozygous
|
|
|
|
| TP53 p.S241F c.722C>T Heterozygous
TP53 p.? c.1101-2A>C Heterozygous
|
|
|
|
| Chek2-deficient 15723659(C)
|
|
|
|
| HCT 116 CCL-247 human colon; colorectal carcinoma epithelial adherent on feeder cells
|
|
|
|
| express Ctnnb1 in nuclear fraction [WB] 16007074-Fig-S3
|
|
|
|
| CDKN2A p.R24fs*20 c.68_69insG Homozygous
|
|
|
|
| CTNNB1 p.S45del c.133_135delTCT Heterozygous
|
|
|
|
| KRAS p.G13D c.38G>A Heterozygous
|
|
|
|
| MLH1 p.S252* c.755C>A Homozygous
|
|
|
|
| PIK3CA p.H1047R c.3140A>G Heterozygous
|
|
|
|
| a KLH specific mouse Th1-cell clone 11046021(C)
|
|
|
|
| Normal human diploid fibroblasts 21149446(C)
|
|
|
|
| HEI 193 is a human schwannoma cell line established from a patient bearing a loss-of-function splice-site mutation in NF2 (20, 21). 16537831(C)
|
|
|
|
| 20. Fraenzer, J. T., Pan, H., Minimo, L., Jr., Smith, G. M., Knauer, D. & Hung, G.
(2003) Int . J. Oncol . 23, 1493–1500.
21. Hung, G., Li, X., Faudoa, R., Xeu, Z., Kluwe, L., Rhim, J. S., Slatter y, W. &
Lim, D. (2002) Int . J. Oncol . 20, 475– 482.
|
|
|
|
| 293 CRL-1573 human epithelial adherent kidney, fetal
|
|
|
|
| contains adenovirus EIA gene 9187108(C)
|
|
|
|
| does not express Pten mRNA 9187108(D) ???
|
|
|
|
| expresses Pten protein 14561749(C) with SCAb
|
|
|
|
| transfection of HEK293 with Pten does not inhibit growth 10698713(D) indicating that it already expresses Pten
|
|
|
|
| It was shown earlier that these cells contain a nonfunctional STAT6 molecule but otherwise an intact IL-4 signal transduction cascade. Therefore, activation of STAT6-dependent genes in these cells can be restored by ectopic expression of functional STAT6 (30, 32, 37).
|
|
|
|
| 30. Hoeck, J., and M. Woisetschla ̈ger. 2001. STAT6 mediates eotaxin-1 expression in IL-4 or TNFa induced fibroblasts. J. Immunol. 166:4507.
|
|
|
|
| 32. Stuetz, A. M., and M. Woisetschla ̈ger. 1999. Functional synergism of STAT6 with either NF-kB or PU.1 to mediate IL-4-induced activation of IgE germline gene transcription. J. Immunol. 163:4383.
|
|
|
|
| 37. Lu, B., M. Reichel, D. A. Fisher, J. F. Smith, and P. Rothman. 1997. Identifi- cation of a STAT6 domain required for IL-4-induced activation of transcription. J. Immunol. 159:1255.
|
|
|
|
| mRNA: Fzd1-low Fzd4-neg Fzd7-pos Fzd10-neg 15923619-Fig-3c
|
|
|
|
| mRNA: Fzd1-low Fzd2-pos Fzd3-pos Fzd4-neg Fzd5-low Fzd6-pos Fzd7-neg Fzd8-neg Fzd9-low Fzd10-neg 18187455-Fig-S2c
|
|
|
|
| used in 9927421(C) with reference
|
|
|
|
| Graham,F.L., Smiley,J., Russell,W.C. and Nairn,R. (1977) Characteristics
of a human cell line transformed by DNA from human adenovirus
type 5. J. Gen. Virol., 36, 59–72.
|
|
|
|
| used without explanation in 16286006 17360704 12906785
|
|
|
|
| HEK293E express E1A 17386226(C)
|
|
|
|
| In HEK293E but not HEK293T cells, the mTORC1 pathway is strongly regulated by serum and insulin 17386226(C)
|
|
|
|
| Compared with HEK293 and HEK293T cells, there are low basal levels of phosphorylation of mTOR targets in HEK293E cells, and thus HEK293E cells were used for insulin signal study. 18372248(C)
|
|
|
|
| Express normal levels of Deptor 19446321(D)
|
|
|
|
| obtained from Invitrogen but Invitrogen doesn't carry them 11116146(C)
|
|
|
|
| 293-H Cells, SFM Adapted Invitrogen SKU# 11631-017
|
|
|
|
| Adapted to CD 293 Medium (Cat. No. 11913-019).293-F and 293-H Cells are clonal isolates, derived from transformed embryonal human kidney cells (1,2). The 293-F strain is a fast-growing variant of the 293 cell line. The 293-H strain is a variant of the 293 cell line that demonstrates better adherence in monolayer culture and ease of use for plaque assays and other anchorage dependent applications. The 293 parental cell line was selected for growth and transfection efficiency (2). Both 293-F and 293-H cell lines can beadapted to serum-free suspension culture in 293 SFM II or CD 293 Medium supplemented with L-glutamine for recombinant protein production and adenovirus propagation.
|
|
|
|
| 293-TK/Zeo cells were expanded to pools of 107 cells each, subjected to five rounds of mutagenesis with ICR191 (an intercalating agent that induces frameshift mutations), and selected in gancyclovir plus IL-1.
|
|
|
|
| turns on Nfkb-reporter or CBS binding irt Tnf but not IL1b
|
|
|
|
| secretes IL8 irt Tnf but not IL1b
|
|
|
|
| lacks Irak1 protein expression
|
|
|
|
| 293-TK/Zeo cells were expanded to pools of 107 cells each, subjected to five rounds of mutagenesis with ICR191 (an intercalating agent that induces frameshift mutations), and selected in gancyclovir plus IL-1.
|
|
|
|
| turns on Nfkb-reporter or CBS binding irt Tnf but not IL1b
|
|
|
|
| secretes IL8 irt Tnf but not IL1b
|
|
|
|
| 293-TK/Zeo cells were expanded to pools of 107 cells each, subjected to five rounds of mutagenesis with ICR191 (an intercalating agent that induces frameshift mutations), and selected in gancyclovir plus IL-1.
|
|
|
|
| turns on Nfkb-reporter or CBS binding irt Tnf but not IL1b
|
|
|
|
| secretes IL8 irt IL1b but not Tnf
|
|
|
|
| lacks Myd88 protein expression
|
|
|
|
| synonyms: 293T Hek 293T HEK293T+
|
|
|
|
| used without explanation in 14690596 11533489 16135789 10755617 14560022
|
|
|
|
| possibly: 293T/17 CRL-11268 human epithelial adherent kidney; highly transfective
|
|
|
|
| lacks endogenous Stat4 10961885(C)
|
|
|
|
| express Ctnnb1 in nuclear fraction [WB] 16007074-Fig-S3
|
|
|
|
| Express lower than average levels of Deptor 19446321(D)
|
|
|
|
| HeLa CCL-2 human cervix; adenocarcinoma epithelial adherent
|
|
|
|
| STK11 p.? c.1_464del464 Homozygous
|
|
|
|
| D98-AH2-CloneB, GLC-82, KB, MR1-3, AKI, J-111, MR10-1, Hep2, Hep-2C
|
|
|
|
| expresses moderate levels of Pten mRNA 9187108(D)
|
|
|
|
| does not express IL2Rg mRNA 1631559(D)
|
|
|
|
| express ArhGef7 but not ArhGef6 9659915-Fig-4
|
|
|
|
| Express normal levels of Deptor 19446321(D)
|
|
|
|
| Reichel, M., B. H. Nelson, P. D. Greenberg, and P. B. Rothman. 1997. The IL-4 receptor alpha-chain cytoplasmic domain is sufficient for activation of JAK-1 and STAT6 and the induction of IL-4-specific gene expression. J. Immunol. 158:
5860.
|
|
|
|
| HeLa S3 CCL-2.2 human cervix; adenocarcinoma epithelial adherent
|
|
|
|
| mRNA: Fzd1-neg Fzd2-pos Fzd3-low Fzd4-neg Fzd5-pos Fzd6-pos Fzd7-neg Fzd8-neg Fzd9-neg Fzd10-low 19910923-Fig-S1
|
|
|
|
| immortalized mouse PECs 16895974
|
|
|
|
| human hepatocellular carcinoma aka Hep 3B2.1-7, Hep-3B, Hep3B
|
|
|
|
| Hep3B is p53-null. 11459832(C)
|
|
|
|
| Hep3B has no Rb protein expression. 11459832(C)
|
|
|
|
| might be: HEPA1C1C7 or HEPA16
|
|
|
|
| Hepa 1-6 CRL-1830 mouse epithelial adherent liver; hepatoma
|
|
|
|
| Hepa-1c1c7 CRL-2026 mouse epithelial adherent liver; hepatoma
|
|
|
|
| human hepatocellular carcinoma aka HEP-G2 Hep G2
|
|
|
|
| does not express IL2Rg mRNA 1631559(D)
|
|
|
|
| expresses mRNA for IL6R and GP130 2261637-Fig-4
|
|
|
|
| expresses Gp130 and LifR 16774741(C)
|
|
|
|
| K.J. Kallen, J. Grotzinger, E. Lelievre, P. Vollmer, D. Aasland, C. Renne, J. Mullberg, K.H. Myer zum Buschenfelde, H. Gascan, S. Rose-John, Receptor recognition sites of cytokines are organized as exchangeable modules. Transfer of the leukemia inhibitory factor receptor-binding site from ciliary neurotrophic factor to interleukin-6, J. Biol. Chem. 274 (1999) 11859–11867.
|
|
|
|
| expresses WT Axin1 19131971(C)
|
|
|
|
| HepG2 carries normal Rb. 11459832(C)
|
|
|
|
| used without explanation in 8524316
|
|
|
|
| High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate derived from the parental Trichopulsia ni cell line (cabbage looper ovary) and are commonly used for the expression of recombinant proteins using the Baculovirus Expression Vector System
|
|
|
|
| Cell line from Trichoplusia ni egg cells. Adherent
|
|
|
|
| HoxB8-immortalized myeloid progenitors 163606937(C)
|
|
|
|
| Fetal liver myeloid progenitors were immortalized using an oestrogen-responsive ER-HoxB8 oncogene in the presence of 1mM oestrogen and GM-CSF [21].
|
|
|
|
| 21. Knloepfler, P. S., Sykes, D. B., Pasillas, M. &Kamps, M. P. HoxB8 requires its Pbx-interaction motif to block differentiation of primary myeloid progenitors and of most cell line models of myeloid differentiation. Oncogene20, 5440-5448(2001).
|
|
|
|
| HK-2 CRL-2190 human epithelial adherent kidney (cortex, proximal tubule); HPV-16 transformed
|
|
|
|
| human renal proximal tubular epithelial cells immortalized by transduction with human papilloma virus 16 E6/E7 genes (21) 15084590(C)
|
|
|
|
| 21. Ryan, M. J., Johnson, G., Kirk, J., Fuerstenberg, S. M., Zager, R. A., and
Torok-Storb, B. (1994) Kidney Int. 45, 48 –57
|
|
|
|
| HL-60 CCL-240 human peripheral blood; promyeloblast; acute myeloblastic suspension promyelocytic leukemia
|
|
|
|
| TP53 p.M1_*394del c.1_1182del1182 Confirmed somatic variant Homozygous
|
|
|
|
| CDKN2A p.R80* c.238C>T Confirmed somatic variant Homozygous
|
|
|
|
| NRAS p.Q61L c.182A>T Confirmed somatic variant Heterozygous
|
|
|
|
| http://www.sanger.ac.uk/perl/genetics/CGP/core_line_viewer?action=sample&id=905938
|
|
|
|
| human hepatocellular carcinoma cells 12872134(C)
|
|
|
|
| human Renal Cell Carcinoma cell line 7721786(C)
|
|
|
|
| Obiri N etal (1993) J Clin Invet 91, 88-93
|
|
|
|
| used without explanation in 12621041
|
|
|
|
| mastocytoma aka mast cell leukemia cell line 12177062(C)
|
|
|
|
| HMC1.1 expresseds Kit V560C mutation
|
|
|
|
| HMC1.2 expresses Kit V560C and D816V mutation
|
|
|
|
| Human microvascular endothelial cell-1
|
|
|
|
| First published by PMID:1361507
|
|
|
|
| Human dermal microvascular endothelial cells (HMEC) immortalized through transfection with SV40 T antigen
|
|
|
|
| human squamous cell carcinoma 11716761(C)
|
|
|
|
| used without explanation in 16630891
|
|
|
|
| possibly: HPAF-II CRL-1997 human epithelial adherent pancreas; adenocarcinoma
|
|
|
|
| 11278283(C) human thymoma derived
|
|
|
|
| HPB-ALL a human T lymphoblastoid cell line 7561682(C)
|
|
|
|
| immortalized human pancreatic duct epithelial cell (HPDEC) line was obtained from Dr. M. S. Tsao (Ontario Cancer Institute, University Health Net- work/Princess Margaret Hospital, Toronto, ON, Canada) 20100905(C)
|
|
|
|
| Ouyang H, Mou Lj, Luk C, Liu N, Karaskova J, Squire J, and Tsao MS (2000) Immortal human pancreatic duct epithelial cell lines with near normal genotype and phenotype. Am J Pathol 157:1623–1631.
|
|
|
|
| HS-24, a tumor cell line with properties of human bronchial EC (1,32), was generously provided by Dr. E. Spiess (Heidelberg, Germany). 15557085(C)
|
|
|
|
| 1. Abe T, Kobayashi N, Yoshimura K, Trapnell BC, Kim H, Hubbard
RC, Brewer MT, Thompson RC, and Crystal RG. Expression of the
secretory leukoprotease inhibitor gene in epithelial cells. J Clin Invest 87:
2207–2215, 1991.
|
|
|
|
| 32. Maruyama MJ, Hay G, Yoshimura K, Chu CS, and Crystal RG.
Modulation of secretory leukoprotease inhibitor gene expression in human
bronchial epithelial cells by phorbol ester. J Clin Invest 94: 368 –375,
1994.
|
|
|
|
| Hs24Fs CRL-7016 human fibroblast adherent skin (foreskin)
|
|
|
|
| human oral squamous cell carcinoma cells 9311995(C)
|
|
|
|
|
Growth Properties:
|
adherent
|
Organism:
|
Homo sapiens
|
Morphology:
|
epithelial
|
|
|
|
|
| Source:
Organ: mammary gland; breast
Disease: carcinoma
|
|
|
|
| expresses low levels of Huwe1 protein 15989956(D)
|
|
|
|
| HT-1080 CCL-121 human epithelial adherent connective tissue; fibrosarcoma
|
|
|
|
| CDKN2A p.M1_*157del c.1_471del471 Homozygous
NRAS p.Q61K c.181C>A Heterozygous
|
|
|
|
| does not express Egr1 11781575(C)
|
|
|
|
| expresses Egr1 14744395(D)
|
|
|
|
| HT-2 clone A5E CRL-1841 mouse lymphoblast suspension spleen; T lymphocyte
|
|
|
|
| IL2 dependent T cell line 11331873(C)
|
|
|
|
| HT-29 HTB-38 human epithelial adherent colon; colorectal adenocarcinoma
|
|
|
|
| colon carcinoma 10197981(C)
|
|
|
|
| APC p.T1556fs*3 c.4666_4667insA Heterozygous
APC p.E853* c.2557G>T Heterozygous
BRAF p.V600E c.1799T>A Heterozygous
PIK3CA p.P449T c.1345C>A Heterozygous
SMAD4 p.Q311* c.931C>T Homozygous
TP53 p.R273H c.818G>A Homozygous
|
|
|
|
| does not express Lef1 or Tcf7 9065401(D)
|
|
|
|
| Express normal levels of Deptor 19446321(D)
|
|
|
|
| The subclone 16E of the human colonic epithelial cell line HT29 undergoes differentiation to the goblet cell-like phenotype after reaching confluence (28). 15004182(C)
|
|
|
|
| 28. Augeron, C., and C. L. Laboisse. 1984. Emergence of permanently differentiated cell clones in a human colonic cancer cell line in culture after treatment with sodium butyrate. Cancer Res. 44:3961.
|
|
|
|
| a HT29 clone selected for high expression of Pigr 11034397(C)
|
|
|
|
| 41. Kvale, D., J. Bartek, L. M. Sollid, and P. Brandtzaeg. 1988. Rapid selection of cultured cells with increased expression of a membrane marker (secretory com- ponent). Int. J. Cancer 42:638.
|
|
|
|
| ECACC catalog code: 93120108
|
|
|
|
| Derived from a rat ascites tumour, which in turn was derived from a solid hepatoma induced in male Buffalo rats by feeding them 0.04% N,N'-2,7fluorenyle-bis-2,2,2-trifluoroacetamide for 12.4 months.
|
|
|
|
| Monoclonal continuous culture, grown as suspension, epithelial-like morphology, Growth also as monolayer
|
|
|
|
| ICLC catalog code: ATL95006
|
|
|
|
| Continuous culture, grown as monolayer, epithelial-like morphology
|
|
|
|
| cervical cancer cells 16005186(C) [15]
|
|
|
|
| [15] S.L. Huang, R.Y. Shyu, M.Y. Yeh, S.Y. Jiang, Anticancer Res. 22
(2002) 799.
|
|
|
|
| a human hepatoma cell line 16301520(C)
|
|
|
|
| a human hepatoma cell line 1150296(C)
|
|
|
|
| 26. Nakabayashi, H., Taketa, K., Miyano, K., Yamane, T., and Sato, J. (1982)
Cancer Res. 42, 3858–3863
|
|
|
|
| a hepatoma cell line 16177806(C)
|
|
|
|
| Huh7 cells expressing the HCV-replicon 16731946(C)
|
|
|
|
| 5. Blight, K. J., A. A. Kolykhalov, and C. M. Rice. 2000. Eficient initiation of HCV RNA replication in cell culture. Science 290:1972–1974.
|
|
|
|
| HuT 78 TIB-161 human lymphoblast suspension T lymphocyte, cutaneous; lymphoma
|
|
|
|
| do not express Cd40 12818164(C)
|
|
|
|
| IEC18 CRL-1589 rat epithelial adherent ileum
|
|
|
|
| a previously characterized, clonal intestinal epithelial cell line which is hypersensitive to TGFP, relative to the parental cells from which it was derived. 4-1 cells were derived by chemical mutagenesis of untransformed rat intestinal epithelial (IEC-18) cells, selection in the presence of TGFP, and limiting dilution cloning, as described previously. 1544886(C)
|
|
|
|
| K. M. Mulder, P. R. Segarini, S. L. Morris, J. M. Ziman, and H. G. Choi (1993) J Cell Physiol 154,162-174.
|
|
|
|
| a clone of IEC18 that is resistant to Tgfb effects 7706248(C)
|
|
|
|
| K. M. Mulder, P. R. Segarini, S. L. Morris, J. M. Ziman, and H. G. Choi (1993) J Cell Physiol 154,162-174.
|
|
|
|
| IIA1.6 a murine B cell line that does not express any of the Tcf genes 9065401(C)
|
|
|
|
| M. Molenaar et al., Cell 86, 396 (1996)
|
|
|
|
| J. Behrens et al., Nature 382, 638 (1996)
|
|
|
|
| O. Huber et al., Mech. Dev. 59, 3 (1996).
|
|
|
|
| a human IL2-dependent T-cell line 9133424(C)
|
|
|
|
| used without explanation in 9363897
|
|
|
|
| Sekine, N., Ullrich, S., Regazzi, R., Pralong, W. F. & Wollheim, C. B. Postreceptor signaling of growth hormone and prolactin and their effects in the differentiated insulin-secreting cell line, INS-1. Endocrinology 137, 1841–1850 (1996).
|
|
|
|
| human lung fibroblasts aka IMR-90
|
|
|
|
| a macrophage cell line 16537926(C)
|
|
|
|
| a mouse BALB/c monocyte/macrophage cell line 15820149(C)
|
|
|
|
| J774A.1 TIB-67 mouse macrophage adherent macrophage; monocyte; reticulum cell sarcoma
|
|
|
|
| mouse macrophage-like cell line
|
|
|
|
| used without explanation in 16763563
|
|
|
|
| JAR HTB-144 human epithelial adherent placenta; choriocarcinoma
|
|
|
|
| ATCC CRL-2010 JB6 CL 41-5a
|
|
|
|
| Normal epithelial cells from BaLB/c mouse skin sensitive to transformation by phorbol esters and other tumor promoters
|
|
|
|
| a Jurkat clone that does not express Lck or Syk 15240695(D) 9488700(C)
|
|
|
|
| rat hepatoma cell line 16952352(C)
|
|
|
|
| This cell line has low endogenous expression of β-catenin and does not have any mutations in its exon-3, a site critical for its phosphorylation and degradation.
|
|
|
|
| [31] D.L. Novicki, R.L. Jirtle, G. Michalopoulos, Establishment of two rat hepatoma cell strains produced by a carcinogen initiation, phenobarbital promotion protocol, In Vitro 19 (1983) 191–202.
|
|
|
|
| Nemo-null, no other info 14695475(C)
|
|
|
|
| JM4.5.2 cells, a T cell line that lacks expression of NEMO (46). 12867425(C)
|
|
|
|
| 46. Harhaj, E. W., Good, L., Xiao, G., Uhlik, M., Cvijic, M. E., Rivera-Walsh, I., and Sun, S. C. (2000) Oncogene 19, 1448 –1456
|
|
|
|
| human T lymphocyte; acute T cell leukemia lymphoblast suspension
|
|
|
|
| does not express Pten 12133897(D) 16603397-Fig-S3
|
|
|
|
| 28. Shan, X., Czar, M. J., Bunnell, S. C., Liu, P., Liu, Y., Schwartzberg, P. L., and Wange, R. L. Deficiency of PTEN in Jurkat T cells causes constitutive localization of Itk to the plasma membrane and hyperresponsiveness to CD3 stimulation. Mol. Cell. Biol. 20: 6945–6957, 2000.
|
|
|
|
| expresses IL2Rg mRNA 1631559(D)
|
|
|
|
| expresses mRNA for Gp130 2261637-Fig-4
|
|
|
|
| does not express mRNA for IL6R 2261637-Fig-4 3136546-Fig-3
|
|
|
|
| contain no detectable IL6 binding sites 3136546-Fig-3
|
|
|
|
| does not express IL6R protein 2788034-Fig-2
|
|
|
|
| Jurkat, Clone E6-1 TIB-152 human lymphoblast suspension T lymphocyte; acute T cell leukemia
|
|
|
|
| lacks expression of Syk as a result of a single nucleotide insertion in the Syk open reading frame, leading to premature translational termination (40).
|
|
|
|
| 40. Fargnoli, J., Burkhardt, A. L., Laverty, M., Kut, S. A., van Oers, N. S., Weiss, A., and
Bolen, J. B. (1995) J. Biol. Chem. 270, 26533–26537
|
|
|
|
| do not express Cd40 12818164(C)
|
|
|
|
| European Collection of Animal Cell Culture (HPACC)
|
|
|
|
| General Cell Collection: H33HJ-JA1
Catalogue No.: 90112119
Cell Line Name: H33HJ-JA1
Keywords: Human lymphoma
Cell Line Description: Derivative of the Jurkat cell line and secretes Interleukin 2. The cells should be handled under laboratory containment level 2.
Species: Human
Tissue: blood
Morphology: Lymphoblast
Growth Mode: Suspension
Subculture Routine: Maintain cultures between 2-9x10,000 cells/ml; 5% CO2; 37°C.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Karyotype: Not specified
Products: Interleukin 2 (IL-2)
Depositor: Obtained from ATCC
Originator: No
Country: USA
References: None specified by depositor
Additional Bibliography: Not specified
Patents: This material is cited in a US and/or other Patent and may not be used to infringe patent claims.
Research Council Deposit: No
Release Conditions: No
|
|
|
|
| Fadd "deficient" Jurkat cells 12107169(C)[23]
|
|
|
|
| 23. Juo, P., Kuo, C. J., Yuan, J., and Blenis, J. (1998) Curr. Biol. 8, 1001–1008
|
|
|
|
| used without description in 10725328
|
|
|
|
| a Jurkat cell line that allows the episomal amplification of the transfected plasmids 10669745(C)
|
|
|
|
| a T-antigen-expressing Jurkat cell line 10669745(C)
|
|
|
|
| Jurkat transfected with SV40 T-antigen
|
|
|
|
| JY (obtained from Dr. Riccardo Dalla-Favera) is an EBV-transformed lymphoblastoid B cell line. 10601358(C)
|
|
|
|
| Huh7 cells expressing the HCV-replicon (gift from Michael Gale) 16301520(C)
|
|
|
|
| 19. Sumpter, R., Jr., Wang, C., Foy, E., Loo, Y. M. & Gale, M., Jr. (2004) J. Virol. 78, 11591–11604.
|
|
|
|
| CCL-243, human, lymphoblast, suspension
pleural effusion (metastatic); chronic myelogenous leukemia (bone marrow primary)
|
|
|
|
| K562 lack the entire locus for type I IFN genes and results in the absence of autocrine IFN expression 15208624(C)
|
|
|
|
| Philadelphia chromosome-positive human cell line 7493940(C)
|
|
|
|
| CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.0? c.1_522del522 Homozygous
TP53 p.Q136fs*13 c.406_407insC Homozygous
|
|
|
|
| "express a mutant EgfR lacking intrinsic protein tryosine kinase activity" 8524316(C)
|
|
|
|
| human oral epithelial carcinoma
|
|
|
|
| KB CCL-17 human epithelial adherent HeLa contaminant
|
|
|
|
| human myeloid cell 15240695(C)
|
|
|
|
| human myelogenous leukemia cell line
|
|
|
|
| TP53 p.? c.672+1G>A Confirmed somatic variant Homozygous
|
|
|
|
| KG1 grown in RPMI/HI-FCS + Csf1 + IL4 for 5 days
|
|
|
|
| KGI grown in RPMI + Csf2 + Tnf + Ionomycin for 1-3 days
|
|
|
|
| IL2 dependent NK cells 15271800(C)
|
|
|
|
| 37. Yagita M, Huang CL, Umehara H, et al. A novel natural killer cell line (KHYG-1) from a patient with aggressive natural killer cell leukemia carrying a p53 point mutation. Leukemia. 2000;14:922- 930.
|
|
|
|
| an IL2 dependent T-cell line 10373548(C)
|
|
|
|
| an IL2 dependent human chronic T lymphocytic leukemic cell line 1940342(C)
|
|
|
|
| 37. Hori. T., T. Uchiyama. M. Tsudo, H. Umadome. H. Ohno, S. Fukuhara,K.Kita,andH. Uchino. 1987.Establishmentof an interleukin2-dependent human T cell line from a patient with T cell chroniclymphocytic leukemia whois not infected with human T cell leukemia/lymphomavirus. Blood 70:1069.
|
|
|
|
| Rb-pos Tp53-neg 9724636(C,D)
|
|
|
|
| KIT225/K6, an IL-2 dependent subclone derived from the human T cell line KIT225 (18), was obtained from Dr. T. Uchiyama (Kyoto, Japan) and previously had been demonstrated to express IL-12Rs (19). 7527811(C)
|
|
|
|
| 19. Desai, B. B., T. Truitt, S. Honasoge, R. Warrier, R. Chizzonite, and
M. Gately. 1993. Expression of functional IL-12 receptor on a hu-
man IL-2-dependent T cell line. J. Immunol. 150t207A.
|
|
|
|
| one of NCI60 - not in ATCC
|
|
|
|
| GNAS p.R201H c.602G>A Heterozygous
KDM6A p.S40fs*2 c.117delC Homozygous
PTEN p.K267fs*9 c.800delA Heterozygous
PTEN p.G129* c.385G>T Heterozygous
TP53 p.R72fs*51 c.215delG Heterozygous
|
|
|
|
| KMS-28BM (WT) and KMS-28PE (c-IAP-/-) multiple myeloma cells, were
a generous gift from Rafael Fonseca, Mayo Clinic (3) 18635759(C)
|
|
|
|
| J. J. Keats et al., Cancer Cell 12, 131 (2007). 17692805
|
|
|
|
| Bornschein et al. J. Cancer Mol. 4(2): 47-54, 2008
|
|
|
|
| typical small intestinal carcinoid
|
|
|
|
| a mouse NK cell clone 10320373(C)
|
|
|
|
| F. M. Karlhofer, M. M. Orihuela, W. M. Yokoyama, J. Exp. Med. 181, 1785 (1995)
|
|
|
|
| L1.2, a murine pre-B lymphoma cell line 9722546(C)
|
|
|
|
| Growth Properties: suspension
Organism: Mus musculus (mouse)
Morphology: lymphoblast
Source: Disease: lymphocytic leukemia
Strain: DBA subline 212
|
|
|
|
| used without explanation in 12034739
|
|
|
|
| "a derivative of MV1LU [24] obtained from J Massague" 12193595(C)
|
|
|
|
| 24. Attisano, L., Carcamo, J., Ventura, F., Weis, F. M. B., Massague´ , J., and Wrana, J. L. (1993) Cell 75, 671– 680
|
|
|
|
| human lymphoblasts from a patient with AT 15788536(C)
|
|
|
|
| lacks the ATM protein 15788536(D)
|
|
|
|
| human Hodgkin's disease-derived cell line strongly expressing IL18R 9325300(C)
|
|
|
|
| a normal human lymphoblastoid cell line 10611322(C)
|
|
|
|
| human Lymphoblasts from a normal donor 9168117(C) 9733514(C) 14532133(C)
|
|
|
|
| lymphoblasts expressing WT Atm 15073328(C)
|
|
|
|
| L6 CRL-1458 rat adherent skeletal muscle myoblast
|
|
|
|
| human lymphoblasts from a patient with AT 9733514(C)
|
|
|
|
| lacks the ATM protein 9733514(D)
|
|
|
|
| NCTC clone 929 CCL-1 mouse fibroblast adherent subcutaneous connective tissue (areolar and adipose)
|
|
|
|
| ATCC supplied synonyms: L-cell, L-929, L929, derivative of Strain L
|
|
|
|
| LMAMb7: L929<xIL4Ra><xIL2Rb>
|
|
|
|
| LMAmb7mg1: L929<xIL4Ra><xIL2Rb><xIL2Rg>
|
|
|
|
| used in 12628924 with no explanation about the "SA"
|
|
|
|
| a cell line of neuronal origin; human neuroblastoma 1850549(C)
|
|
|
|
| L Cells CRL-2648 mouse fibroblast adherent subcutaneous connective tissue, areolar and adipose; control for L Wnt-3A cells
|
|
|
|
| The cell line named L Cells is closely related or identical to L-M(TK-) cells (ATCCCCL-1.3).
|
|
|
|
| expresses IL4R 8266078(C)
|
|
|
|
| does not express IL2g 8266078(C)
|
|
|
|
| PCNA express NgfR (16) 8614802(C)
|
|
|
|
| M.J. Radeke, T. P. Misko,C. Hsu, L.A. Herzenberg, E. M. Shooter, Nature 325, 593 (1987).
|
|
|
|
| Tsc2-null LExF2 cells (LEF) were maintained in DMEM/F12 (Invitrogen)
containing 10% FBS and 50 mg/mL P/S (Inoki et al., 2003). 16959574(C)
|
|
|
|
| Inoki, K., Zhu, T., and Guan, K.L. (2003). TSC2 mediates cellular energy
response to control cell growth and survival. Cell 115, 577–590.
|
|
|
|
| The LFS 041 fibroblast strain MDAH041 (p53-null, harboring a codon 184 frameshift mutation that results in premature termination of translation of the p53 protein) was kindly provided by Dr. Michael Tainsky (M.D. Anderson Cancer Center, Hous- ton, TX). 11340074(C)
|
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|
| LLC-MK2 CCL-7.1 monkey, Rhesus kidney epithelial adherent
|
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|
|
| LLC-PK1 CL-101 pig kidney epithelial adherent
|
|
|
|
| a human osteosarcoma cell line with high potential in lung metastases as described previously (19). 16380506(C)
|
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|
|
| 19. Jia S-F, Worth LL, Kleinerman ES. A nude mouse model of human osteosarcoma lung metastases for evaluating new therapeutic strategies. Clin Exp Metastasis 1999;17:501–6.
|
|
|
|
| LN-229 CRL-2611 human epithelial adherent brain; glioblastoma; apoptosis studies; p53+, p16–, p14ARF–
|
|
|
|
| LNCaP cl.FGC CRL-1740 human lymph node (metastasis); carcinoma (prostate epithelial loosely adherent primary) clusters
|
|
|
|
| colon carcinoma 10197981(C)
|
|
|
|
| Kras-G12D Tp53-null Apc-null 10197981(C)
|
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|
|
| LoVo CCL-229 human lymph node, left supraclavicular region (metastasis); colorectal adenocarcinoma (colon primary) epithelial adherent
|
|
|
|
| LS 174T CL-188 human epithelial adherent colon; colorectal adenocarcinoma
|
|
|
|
| M059J CRL-2366 human fibroblast adherent brain, glial cell; malignant glioblastoma; glioma
|
|
|
|
| null for DNA-PKcs 15718470(C) 15723659(C)
|
|
|
|
| M1 TIB-192 mouse myeloblast; myeloid leukemia suspension
|
|
|
|
| expresses very low levels of IL6R 2788034-Table-1
|
|
|
|
| M12.4.1 a B cell line 15084608(C)[31]
|
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|
| 31. Hamano, T., Kim, K. J., Leiserson, W. M., and Asofsky, R. (1982) J. Immunol.
129, 1403–1406
|
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|
| a transformed B cell line 10072514(C)
|
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|
|
| 8. Rothman, P., S. Li, B. Gorham, L. Glimcher, F. Alt, and M. Boothby. 1991. Identification of a conserved LPS/IL-4 responsive element located at the pro-
moter of germline epsilon transcripts. Mol. Cell. Biol. 11:5551.
|
|
|
|
| expressed low levels of endogenous Fes 11160325(C)
|
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|
|
| used without explanation in 11714803 12354380
|
|
|
|
| 16537510(C): B lymphoma cell line
|
|
|
|
| does not express IL6R 2788034-Table-1
|
|
|
|
| contains Lkb1 protein 19187764-Fig-6d
|
|
|
|
| Cosmic id 905975 same donor as MDAMB435
|
|
|
|
| BRAF p.V600E c.1799T>A Heterozygous
CDKN2A p.? c.150+2T>C Heterozygous
CDKN2A p.? c.456_457+25AGGTGAGGACTGATGATC TGAGAATTT>C Heterozygous
TP53 p.G266E c.797G>A Heterozygous
|
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|
|
| testiculare cell line derived from a mouse leydig cell tumor 10854429(C)
|
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|
| 21. Ascoli, M. (1981) Endocrinology 108, 88 –95
|
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|
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| a murine bone marrow macrophage cell line established by infection with retroviruses carying v-raf and v-myc oncogenes 17618271(C)
|
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|
|
| Blasi, E. et al. Selective immortalization of murine macrophages from fresh bone
marrow by a raf/myc recombinant murine retrovirus. Nature 318, 667-670 (1985).
|
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|
|
| Malme-3M HTB-64 human skin; malignant melanoma mixed adherent
|
|
|
|
| contains Lkb1 protein 19187764-Fig-6d
|
|
|
|
| human Renal Cell Carcinoma cell line 7721786(C)
|
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|
|
| Obiri N etal (1993) J Clin Invet 91, 88-93
|
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|
|
| MC3T3-E1 CRL-2594 mouse bone (calvaria); osteoblast differentiation model fibroblast adherent Subclone 14
MC3T3-E1 CRL-2595 mouse bone (calvaria); osteoblast differentiation model fibroblast adherent Subclone 24
MC3T3-E1 CRL-2596 mouse bone (calvaria); osteoblast differentiation model fibroblast adherent Subclone 30
MC3T3-E1 CRL-2593 mouse bone (calvaria); osteoblast differentiation model fibroblast adherent Subclone 4
|
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|
|
| mouse calvarial cell line
|
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|
|
| the only major BMP7 type I receptor expressed on this cell line is ALK2 - 9748228(C)
|
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|
|
| ten Dijke, P., Yamashita, H., Sampath, T. K., Reddi, A. H., Estevez, M., Riddle, D. L., Ichijo, H., Heldin, C.-H., and Miyazono, K. (1994) J. Biol. Chem. 269, 16985–6988
|
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|
|
| derived by Sudo et al. (44) - 9748228(C)
|
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|
| 44. Sudo, H., Kodama, H., Amagi, Y., Yamamoto, S., and Kasai, S. (1983) J. Cell Biol. 96, 191–198
|
|
|
|
| MC/9 CRL-8306 mouse lymphoblast suspension liver, mast cell
|
|
|
|
| Mast cells line 9525930(C)
|
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|
|
| MCF 10A CRL-10317 human epithelial adherent mammary gland; fibrocystic disease
|
|
|
|
| do not express Huwe1 protein 15989956(D)
|
|
|
|
| express normal levels of Pin1 11432833-Fig-2d
|
|
|
|
| MCF-12A CRL-10782 human epithelial adherent mammary gland
|
|
|
|
| ATCC HTB-22 human pleural effusion (metastasis); adenocarcinoma epithelial adherent
|
|
|
|
| Published comments and data
|
|
|
|
| does not express Stat6 [Northern] 7760829-Fig-2
|
|
|
|
| express endogenous Pin1 11533658-Fig-1c
|
|
|
|
| wild-type for both pRb and p53 function 9724636(C)
|
|
|
|
| express Huwe1 protein 15989956(D)
|
|
|
|
| express high levels of Pin1 11432833-Fig-2d
|
|
|
|
| p53-positive [RTPCR] 16676006(D)
|
|
|
|
| CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.? c.317_522del206 Homozygous
PIK3CA p.E545K c.1633G>A Heterozygous
|
|
|
|
| synonyms: MCF-7S1 MCF7-S1
|
|
|
|
| used without explanation in 17244528 12058026
|
|
|
|
| used without explanation in 11894095
|
|
|
|
| human fibroblast line 14560022(C)
|
|
|
|
| ATCC HTB-26 human adenocarcinoma adherent pleural effusion (metastasis)
|
|
|
|
| Published comments and data
|
|
|
|
| derived from ER-negative breast cancer tissue 16596714(C)
|
|
|
|
| overexpress Epha2 16596714(C)
|
|
|
|
| BRAF p.G464V c.1391G>T Heterozygous
CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.0? c.1_522del522 Homozygous
KRAS p.G13D c.38G>A Heterozygous
NF2 p.E231* c.691G>T Homozygous
TP53 p.R280K c.839G>A Homozygous
|
|
|
|
| MDA-MB-361 HTB-27 human brain (metastasis); adenocarcinoma (mammary epithelial loosely adherent gland primary)
|
|
|
|
| MDA-MB-361 HTB-27 human brain (metastasis); adenocarcinoma (mammary epithelial loosely adherent gland primary)
|
|
|
|
| CDKN2A p.M52I c.156G>C Homozygous
PIK3CA p.E545K c.1633G> Heterozygous
|
|
|
|
| previously described as: mammary gland; breast
Disease: previously described as ductal carcinoma
Derived from metastatic site: pleural effusion
|
|
|
|
| This cell line was originally described as a spindle shaped variant of the parental MDA-MB-435 strain isolated in 1976 by R. Cailleau, et al. from the pleural effusion of a 31 year old female with metastatic, ductal adenocarcinoma of the breast. However, recent studies have generated questions about the origin of the parent cell line, MDA-MB-435, and by extension HTB-129. Gene expression analysis of the cells produced microarrays in which MDA-MB-435 clustered with cell lines of melanoma origin instead of breast [PubMed ID: 10700174, PubMed ID: 15150101, PubMed ID: 15679052]. Additional studies have since corroborated a melanocyte origin of MDA-MB-435, to which ATCC has responded by pursuing its own investigation into the identity of this cell line. The cell line to which MDA-MB-435 is reported to have been cross-contaminated with is the M14 melanoma line [PubMed ID: 12354931 and PubMed ID: 17004106].
|
|
|
|
| Cosmic id 905975 same donor as M14
|
|
|
|
| BRAF p.V600E c.1799T>A Heterozygous
CDKN2A p.? c.150+2T>C Heterozygous
CDKN2A p.? c.456_457+25AGGTGAGGACTGATGATC TGAGAATTT>C Heterozygous
TP53 p.G266E c.797G>A Heterozygous
|
|
|
|
| expresses Huwe1 protein 15989956(D)
|
|
|
|
| express high levels of Pin1 11432833-Fig-2d
|
|
|
|
| Express normal levels of Deptor 19446321(D)
|
|
|
|
| MDA-MB-453 HTB-131 human epithelial adherent pleural effusion (metastasis); carcinoma (mammary gland primary)
|
|
|
|
| CDH1 p.W638* c.1913G>A Homozygous
PIK3CA p.H1047R c.3140A>G Heterozygous
|
|
|
|
| known to express HER2, HER3 and HER4, but not HER1 (Plowman et al., 1993a). 9135143(C)
|
|
|
|
| Plowman,G.D., Culouscou,J.-M., Whitney,G.S., Green,J.M., Carlton,G.W., Foy,L., Neubauer,M.G. and Shoyab,M. (1993a) Ligand- specific activation of HER4/p180erbB4, a fourth member of the epidermal growth factor receptor family. Proc. Natl Acad. Sci. USA, 90, 1746–1750.
|
|
|
|
| express high levels of Huwe1 protein 15989956(D)
|
|
|
|
| MDA-MB-468 HTB-132 human epithelial adherent pleural effusion (metastasis); adenocarcinoma (mammary gland primary)
|
|
|
|
| PTEN p.? c.253+1G>T Homozygous
RB1 p.? c.265_2787del2523 Homozygous
SMAD4 p.? c.1_1659del1659 Homozygous
TP53 p.R273H c.818G>A Homozygous
|
|
|
|
| expresses Huwe1 protein 15989956(D)
|
|
|
|
| MDCK (NBL-2) CCL-34 dog epithelial adherent kidney
|
|
|
|
| ATCC: MDCK.2 CRL-2936 Canis familiaris epithelial-like
|
|
|
|
| Cell line was derived by cloning (limited dilution) the parental cell line MDCK (CCL-34. This cell line is susceptible to a wide range of influenza virus and is sensitive to epsilon toxin of C. perfringens.
|
|
|
|
| Tp53-null, Mdm-null mEfs 11331603(C)
|
|
|
|
| Tp53-null mEFs 11331603(C)
|
|
|
|
| MEG-01 CRL-2021 human lymphoblast mixed bone marrow; megakaryoblast; chronic myelogenous leukemia
|
|
|
|
| MeWo HTB-65 human fibroblast adherent lymph node (metastasis); malignant melanoma (skin primary)
|
|
|
|
| contains WT Braf 19274086(C)
|
|
|
|
| contains Lkb1 protein 19187764-Fig-1a
|
|
|
|
| ATCC CRL-1427 human osteosarcoma adherent
|
|
|
|
| used without explanation in 11312266
|
|
|
|
| MIA PaCa-2 CRL-1420 human epithelial adherent pancreas; carcinoma
|
|
|
|
| mIMCD-3 CRL-2123 mouse epithelial adherent (transgenic) kidney (medulla, collecting duct)
|
|
|
|
| immortalized mIMCD-3 cells of ureteric bud origin (10) that are known to express the c-Met receptor and undergo striking tubulogenesis in response to HGF (4). 11784715(C)
|
|
|
|
| 10. Rauchman, M., Nigam, S., Delpire, E., and Gullans, S. (1993) Am. J. Physiol.
F416 –F424
|
|
|
|
| 4. Cantley, L. G., Barros, E. J., Gandhi, M., Rauchman, M., and Nigam, S. K. (1994) Am. J. Physiol. 267, F271–F280
|
|
|
|
| used without explanation in 17287212
|
|
|
|
| Human gastric cancer 19910923(C)
|
|
|
|
| Human gastric adenocarcinoma cell line MKN74 was kindly provided by Dr. E. Tahara (Hiroshima University School of Medicine). MKN74 cells were cultured to subconfluence in RPMI 1640 supplemented with 10% fetal calf serum. 11352916(C)
|
|
|
|
| human myeloblastic leukemia 9187108(C)
|
|
|
|
| expresses high levels of Pten mRNA 9187108(D)
|
|
|
|
| myeloid leukaemia cell line 9153395(C)
|
|
|
|
| MLA 144 cells T-lymphocytes 7721786(C)
|
|
|
|
| does not bind IL13 7721786-Table-1
|
|
|
|
| mouse ATII cell-derived cell line
|
|
|
|
| Dex-sensitive MM.1S and Dex-resistant MM.1R human MM cell lines were kindly provided by... 15010462(C)
|
|
|
|
| Primary fibroblast cells from a classical Menkes disease patient were immortalized by the SV40 gene transfer to derive Me32aT22/2L (43) and designated MNK(Del). The 4.6-kb cDNA encoding the human MNK protein was cloned into a mammalian expression vector and transfected into Me32aT22/2L, and MNK-expressing clone A12-H9 was designated MNK(++) (43). The empty mammalian expression vector, pCMB77, was transfected alone into Me32aT22/2L to derive Me32aT22/2L(pCMB77) and designated MNK(v/o) (43).
|
|
|
|
| 43. La Fontaine, S. L., Firth, S. D., Camakaris, J., Englezou, A., Theophilos, M. B., Petris, M. J., Howie, M., Lockhart, P. J., Greenough, M., Brooks, H., Reddel, R. R., and Mercer, J. F. (1998) J. Biol. Chem. 273, 31375–31380
|
|
|
|
| Mono Mac 6 is a useful tool, which represents the only mature human monocytic cell line available to date www.monocytes.de site is maintained by Loems Ziegler-Heitbrock
|
|
|
|
| suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Disease: acute lymphoblastic leukemia
Cell Type: T lymphoblast
|
|
|
|
| acute T-cell lymphoblastic leukemia 9374471(C)
|
|
|
|
| MOLT-4 CRL-1582 human T lymphoblast; acute lymphoblastic leukemia lymphoblast, suspension
|
|
|
|
| expresses IL2Rg mRNA 1631559(D)
|
|
|
|
| does not bind IL13 7721786(D)
|
|
|
|
| CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.0? c.1_522del522 Homozygous
NOTCH1 p.P2515fs*4 c.7544_7545delCT Heterozygous
NRAS p.G12C c.34G>T Heterozygous
PIK3R1 p.Y452_Q455>SGGSRIK c.1355_1363>CTGGAGGGAGTAGGATTA Heterozygous PTEN p.K267fs*9 c.800delA Homozygous
TP53 p.R306* c.916C>T Heterozygous
|
|
|
|
| MOLTb12, MOLTb: Molt4<xIL2Rb>
|
|
|
|
| expresses endogenous IL2Rg resulting in 6100 sites/cell of intermediate affinity IL2R 1618313(C)
|
|
|
|
| [6] Takeshita, T., Ohtani, K., Asao, H., Kumaki, S., Nakamura, Mand Sugamura, K. (1992) J. lmmunol. 148, 2157
|
|
|
|
| expresses IL2Rg mRNA 1631559(D)
|
|
|
|
| MRC5 CCL-171 human fibroblast adherent lung
|
|
|
|
| human thyroid cancer cells from GF Juillard (University of California at Los Angeles) 14729949(C)
|
|
|
|
| expresses high level of Rela protein but low levels of Pten 14729949(D)
|
|
|
|
| A murine spleen endothelial cell line 16620780(C)
|
|
|
|
| [18] N. Yanai, T. Satoh, M. Obinata, Endothelial cells create a hemato- poietic inductive microenvironment preferential to erythropoiesis in the mouse spleen, Cell Struct. Funct. 16 (1991) 87–93.
|
|
|
|
| an HTLV1 transformed human T-cell line 1631559(C)
|
|
|
|
| expresses IL2Rg mRNA 1631559(D)
|
|
|
|
| expresses IL2Ra mRNA but not IL2Rb 1940342(C)
|
|
|
|
| IL2 independent 1940342(C)
|
|
|
|
| an HTLV1 transformed human T-cell line 1618313(C)
|
|
|
|
| expresses IL2Rg mRNA 1631559(D)
|
|
|
|
| does not express Lck 2047859-Fig-1
|
|
|
|
| MTLn3 rat mammary adenocarcinoma cell line 15337778(C)
|
|
|
|
| Mv1Lu (NBL-7) CCL-64 mink lung epithelial adherent
|
|
|
|
| mink lung epithelial cells 9311995(C)
|
|
|
|
| express: Smad2 Smad3 Smad4 (Smad1 and/or Smad5)
|
|
|
|
| used without explanation in 14750902 12904571 15241417
|
|
|
|
| MV-4-11 CRL-9591 human lymphoblast suspension peripheral blood; biphenotypic B myelomonocytic leukemia
|
|
|
|
| a tumor T cell line established from a plaque biopsy specimen of a petients with mycosis fungoides 12543077(C)
|
|
|
|
| 9. Nielsen M, Kaltoft K, Nordahl M, Ropke C, Geisler C,Mustelin T, Dobson P, Svejgaard A, Odum N (1997) Constitutiveactivation of a slowly migrating isoform of Stat3 in mycosisfungoides: tyrphostin AG490 inhibits Stat3 activation and growthof mycosis fungoides tumor cell lines. Proc Natl Acad Sci USA94:6764–6769.
|
|
|
|
| 42. Kaltoft K, Bisballe S, Dyrberg T, Boel E, Rasmussen PB,Thestrup-Pedersen K (1992) Establishment of two continuous T-cell strains from a single plaque of a patient with mycosis fungoides. InVitro Cell Dev Biol 28A:161–167.
|
|
|
|
| pre-B cell line 9020138(C)
|
|
|
|
| a human B cell line 8995252(C)
|
|
|
|
| Rat T lymphocytes 7479881(C)
|
|
|
|
| human leukemia cells 10022926(C)
|
|
|
|
| NB-4 (human, bone marrow, leukemia,acute promyelocytic)
|
|
|
|
| DSMZ catalog code: ACC 207
|
|
|
|
| Lymphoblastoid cell line established from a patient with NBS 16858402(C)
|
|
|
|
| does not express Nbs1 16858402-Fig-4A
|
|
|
|
| a Nbs1-deficient cell line 11877376(C)
|
|
|
|
| NBS-ILB1 is an SV40-transformed fibroblast cell line established from an NBS patient homozygous for the common founder mutation 657del5 (28) 15234984(C)
|
|
|
|
| 28. Kraakman-van der Zwet, M., Overkamp, W. J. I., Friedl, A. A., Klein, B., Verhaugh, G. W. C. T., Jaspers, N. G. J., Midro, A. T., Eckardt-Schupp, F.,
Lohman, P. H. M., and Zdzienicka, M. Z. (1999) Mutat. Res. 434, 17–27
|
|
|
|
| a Nbs1-deficient cell line 18678890(C)
|
|
|
|
| NBT-II CRL-1655 rat epithelial adherent lymphoma urinary bladder; tumor
|
|
|
|
| ATCC CRL-5800 adenocarcinoma; non-small cell lung cancer
|
|
|
|
| Comments:
This line was derived from a lung cancer obtained from a patient prior to therapy.
The cells carry the K-ras 12 mutation, and there is a mutation in codon 246 (ATC -> ATG, isoleucine -> methionine) of the p53 gene.
There is expression of C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras and N-ras RNAs.
The cells express heterogeneous mRNA expression for PDGF A and B chain, transforming growth factor alpha and beta and the epidermal growth factor receptor (EGFR).
NCI-H23 exhibits a high degree of c-myc DNA amplification (20-fold) but no detectable amplification of c-myc RNA.
The cells stain positive for keratins 5+8 and 18 and vimentin but are negative for neurofilament.
NCI-H23 cells are L-dopa decarboxylase-negative.
They have a reported colony forming efficiency of 9.7% in soft agarose.
|
|
|
|
| ATCC HTB-175 carcinoma; small cell lung cance
|
|
|
|
| ATCC CRL-5810 adenocarcinoma; non-small cell lung cancer
|
|
|
|
|
Growth Properties:
|
adherent, single cells and loosely attached clusters
|
|
|
|
|
| This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lung cancer obtained from a patient prior to therapy.The patient was a smoker (60-pack-year).
The cells carry the K-ras 12 mutation, and there is a mutation in codon 191 of the p53 gene.
The cells are not able to synthesize the peptide neuromedin B (NMB) or the gastrin releasing peptide (GRP).
The cells stain positive for keratin and vimentin but are negative for neurofilament triplet protein.
|
|
|
|
| CRL-5844 human lymph node (metastasis); adenocarcinoma; non-adherent small cell lung cancer (lung primary)
|
|
|
|
| expresses high levels of endogenous Rela protein 14729949(D)
|
|
|
|
| Neuro-2a CCL-131 mouse brain, neuroblast; neuroblastoma neuronal and adherent amoeboid stem cells
|
|
|
|
| a control for F239HTERT 21149446(C)
|
|
|
|
| a neuronal/glioma hybridoma cell line, NG108-15, that stably expresses EphB2 receptor 1150334(C)
|
|
|
|
| Normal hyman fibroblast cell line derived from neonatal skin purchased from Cllonetics 11030628(C)
|
|
|
|
| NIH3T3 CRL-1658 mouse fibroblast adherent embryo
|
|
|
|
| express ArhGef7 but not ArhGef6 9659915-Fig-4
|
|
|
|
| NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3-null><Lck-null>
|
|
|
|
| expresses Jak1 but not Jak3 9512511(C)
|
|
|
|
| Miyazaki, T., A. Kawahara, H. Fujii, Y. Nakagawa, Y. Minami, Z.J. Liu,I. Oishi, O. Silvennoinen, B.A. Witthuhn, J.N. Ihle, and T. Taniguchi. 1994. Functional activation of Jak1 and Jak3 by selective associationwith IL-2 receptor subunits. Science 266: 1045–1047.
|
|
|
|
| fibroblasts which express all three IL-2R subunits and Jak1 but which lack Jak3 and Lck (3, 29). 10825200(C)
|
|
|
|
| 3. Chen, M., A. Cheng, Y. Q. Chen, A. Hymel, E. P. Hanson, L. Kimmel, Y.Minami, T. Taniguchi, P. S. Changelian, and J. J. O’Shea. 1997. The aminoterminus of JAK3 is necessary and sufficient for binding to the commongamma chain and confers the ability to transmit interleukin 2-mediatedsignals. Proc. Natl. Acad. Sci. USA 94:6910–6915.
|
|
|
|
| 29. Minami, Y., I. Oishi, Z. J. Liu, S. Nakagawa, T. Miyazaki, and T. Taniguchi.1994. Signal transduction mediated by the reconstituted IL-2 receptor. Ev-idence for a cell type-specific function of IL-2 receptor beta-chain. J. Immu-nol. 152:5680–5690.
|
|
|
|
| NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3-null><Lck-null><xFak2>
|
|
|
|
| NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3-null><Lck-null><xJak3>
|
|
|
|
| a NIH3T3 derived cell line J3 which expresses low levels of endogenous Fak2 (data not shown) and proliferates in reponse to IL2 stimulation 9512511(C)
|
|
|
|
| Miyazaki, T., A. Kawahara, H. Fujii, Y. Nakagawa, Y. Minami, Z.J. Liu,I. Oishi, O. Silvennoinen, B.A. Witthuhn, J.N. Ihle, and T. Taniguchi. 1994. Functional activation of Jak1 and Jak3 by selective associationwith IL-2 receptor subunits. Science 266: 1045–1047.
|
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|
|
| NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3-null><Lck-null><xJak3><xFak2>
|
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|
|
| NIH3T3<xIL2Ra><xIL2Rb><xIL2Rg><Jak3-null><Lck-null><xJak3><xFak2(mnr)"DN">
|
|
|
|
| differentiated into adipocytes: 7739560(M):
|
|
|
|
| 3T3-L1 fibroblasts were maintained in DMEM containing penicillin-streptomycin plus 10% calf serum and incubated at 37oC in an 8% CO2/air gas mixture.
3T3-L1 fibroblasts (2 days postconfluent) were differentiated into adipocytes by replacing the medium with DMEM containing penicillin-streptomycin, 10% fetal bovine serum, 0.25 uM dexamethasone, 500 uM isobutylmethylxanthine, and 1 ug/ml insulin for 3 days. The 3T3-L1 cells were then incubated with DMEM containing 100 U of penicillin per ml, 100 ug of streptomycin per ml, and 10% fetal bovine serum for 8 to 40 days to maintain the adipocyte phenotype.
|
|
|
|
| NK 3.3 used in 7499365 without explaination
|
|
|
|
| a human NK cell line provided by... 10849428(C)
|
|
|
|
| 18. Bacon, C. M., McVicar, D. W., Ortaldo, J. R., Rees, R. C., O’Shea, J. J., and
Johnston, J. A. (1995) J. Exp. Med. 181, 399–404
|
|
|
|
| an IL2 dependent NK cell line 12323431(C)
|
|
|
|
| NK-92 CRL-2407 human natural killer cell; IL-2 dependent, cytotoxic to lymphoblast clusters in a wide range of malignant cells suspension
|
|
|
|
| NKL is an IL-2-dependent, neoplastic NK cell line derived from a patient with CD3- CD16+ CD56+ large granular lymphocyte leukemia [7544001(M)](37).
|
|
|
|
| 37. Robertson, M. J., Cochran, K. J., Cameron, C., Le, J.-M., Tantravahi, R. & Ritz, J. (1995) Exp. Hematol., in press.
|
|
|
|
| NMuMG CRL-1636 mouse epithelial adherent mammary gland
|
|
|
|
| was previously shown to recapitulate the properties of primary alveolar macrophages. 14525767(C)
|
|
|
|
| 25. Sevilla L, Zaldumbide A, Carlotti F, Dayem MA, Pognonec P, Boulukos KE. Bcl-XL expression correlates with primary macrophage differentia- tion, activation of functional competence, and sur- vival and results from synergistic transcriptional activation by Ets2 and PU.1. J Biol Chem. 2001; 276:17800-17807.
|
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|
|
| murine fibroblast cell line 8621573(C)
|
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|
|
| used without explanation in 14747473(C)
|
|
|
|
| rat kidney cell line 9809973(C)
|
|
|
|
| NTERA-2 cl.D1 CRL-1973 human fibroblast adherent lung (metastasis); malignant; pluripotent embryonal carcinoma (testis primary)
|
|
|
|
| Do not express Nfkb or Ikba 7878466(C)
|
|
|
|
| HDAC-inhibitor resistant melanoma cell line 16481475(C)
|
|
|
|
| HDAC-inhibitor resistant melanoma cell line 16481475(C)
|
|
|
|
| a multiple myeloma cell line 19446321(C)
|
|
|
|
| express normal levels of Mtor Pten Akt1 S6k1 19946321(D)
|
|
|
|
| express higher than normal levels of Deptor 19946321(D)
|
|
|
|
| a MM cell line with a translocation involving the c-MAF gene (Chesi et al., 1998). 19446321(C)
|
|
|
|
| Chesi, M., Bergsagel, P.L., Shonukan, O.O., Martelli, M.L., Brents, L.A., Chen, T., Schrock, E., Ried, T., and Kuehl, W.M. (1998). Frequent dysregulation of the c- maf proto-oncogene at 16q23 by translocation to an Ig locus in multiple myeloma. Blood 91, 4457–4463.
|
|
|
|
| Olf 4.4.2 mouse olfactory epithelial cell line 15023529(C)
|
|
|
|
| O. Bernard, Immortalization of neural cells by the c-myc, N-myc proto-oncogenes, NeuroProtocols 3 (1993) 200–213.
|
|
|
|
| OsA-CL osteosarcoma cells 17085434(C)
|
|
|
|
| 10. Knockaert, M., Sapkota, G., Alarcon, C., Massague´, J., and Brivanlou, A. H. (2006) Proc. Natl. Acad. Sci. U. S. A. 103, 11940 –11945
|
|
|
|
| used in 11231577 without explanation
|
|
|
|
| one of NCI60 - not in ATCC
|
|
|
|
| ERBB2 p.G776V c.2327G>T Heterozygous
TP53 p.? c.376-1G>A Homozygous
|
|
|
|
| P19 CRL-1825 mouse epithelial adherent embryo; teratocarcinoma; embryonic carcinoma
|
|
|
|
| ATCC CCL46 mouse lymphoblast lymphoma
|
|
|
|
| Burkitt’s lymphoma cell line 14563837(C)
|
|
|
|
| contains a Myc T58 mutation 14563837(C)
|
|
|
|
| 34. Niklinski, J., Claassen, G., Meyers, C., Gregory, M. A., Allegra, C. J., Kaye,
F. J., Hann, S. R., and Zajac-Kaye, M. (2000) Mol. Cell. Biol. 20, 5276–5284
|
|
|
|
| cell line derived from rat pulmonary vascular smooth muscle 15923619(C)
|
|
|
|
| mRNA: Fzd1-low Fzd4-pos Fzd7-pos Fzd10-low 15923619-Fig-3c
|
|
|
|
| used in 16630891 without explanation
|
|
|
|
| porcine aortic endothelial cells 9637919(C)
|
|
|
|
| PANC-1 CRL-1469 human epithelial adherent pancreas (duct); epithelioid carcinoma
|
|
|
|
| human pancreatic cancer 17909010(C)
|
|
|
|
| PC-12 CRL-1721 rat adrenal gland; pheochromocytoma polygonal loosely adherent clusters
|
|
|
|
| expresses mRNA for Mlk1 Mlk2 Mlk3 Dlk Lzk GAPDH cyclophilin 11494869(D)
|
|
|
|
| expresses high levels of Pten mRNA 9187108(D)
|
|
|
|
| does not express Stat6 [Northern] 7760829-Fig-2
|
|
|
|
| express mRNA for Fzd2, Fzd5, Fzd7 not Fzd1, Fzd3, Fzd4, Fzd6, Fzd8, Fzd9, Fzd10 12857724-Fig-3a
|
|
|
|
| express mRNA for Lrp6 not Lrp5 12857724-Fig-5a
|
|
|
|
| express Kras and Nras but no detectable Hras protein 16481401-Fig3c
|
|
|
|
| Rat pheochromocytoma (PC12) cells express both high and low affinity NGF binding sites and express both trkA and p75NGFR. 1280261-Fig-2
|
|
|
|
| NGF binding sites per cell: 1849459-Table-1
|
|
|
|
| at 4oC 3.11 x 10*3 high affinity 3.6 x 10*4 low affinity
|
|
|
|
| at 37oC 4.6 x 10*3 high affinity 4.4 x 10*4 low affinity
|
|
|
|
| Ngf-nonresponsive PC12 mutant cell line[11] 7510697(C)
|
|
|
|
| 3005338 11. Green, S.,Rydel, R. E., Connolly, J. L., and Greene. L. A. (1986) J. Cell Biol. 102, 830-843
|
|
|
|
| a PC12 cells variant only expressing p75 receptor but not TrkA 11953455(C)
|
|
|
|
| PC-3 CRL-1435 human epithelial adherent bone (metastasis); adenocarcinoma (prostate primary)
|
|
|
|
| Express abnormally low levels of Deptor 19446321(D)
|
|
|
|
| Sample
Gene
AA Mutation
CDS Mutation
Somatic Status
Zygosity
Vaildated
905934
PTEN
p.R55fs*1
c.165_1212del1048
Variant of unknown origin
Homozygous
Verified
905934
TP53
p.K139fs*31
c.414delC
Variant of unknown origin
Homozygous
Verified
|
|
|
|
| a subline originating from PC3 cells [4] 10801443(C) 22069318(C)
|
|
|
|
| 4. Franzén P, Ichijo H, Miyazono K: Different signals mediate transforming growth factor-β1-induced growth inhibition and extracellular matrix production in prostatic carcinoma cells. Exp Cell Res1993, 207:1-7.
|
|
|
|
| PC6 is a subline of PC12 cells that produces neurites in response to NGF but also grows isolated cells, rather than clumps of cells (42) (generous gift of T. Vanaman, University of Kentucky,
Lexington). 15632082(C)
|
|
|
|
| 42. Pittman, R. N., S. Wang, A. J. DiBenedetto, and J. C. Mills. 1993. A system
for characterizing cellular and molecular events in programmed neuronal
cell death. J. Neurosci. 13:3669–3680.
|
|
|
|
| a subline of neuronal PC12 pheochromocytoma cells 16129692(C)
|
|
|
|
| HNC cell line PCI-15B was established at the University of Pittsburgh School of Medicine [24]. 20426488(C)
|
|
|
|
| (24) Heo, D. S.; Snyderman, C.; Gollin, S. M.; Pan, S.; Walker, E.; Deka, R.; Barnes, E. L.; Johnson, J. T.; Herberman, R. B.; Whiteside, T. L. Biology, cytogenetics, and sensitivity to immunological effector cells of new head and neck squamous cell carcinoma lines. Cancer Res. 1989, 49, 5167–5175.
|
|
|
|
| human Renal Cell Carcinoma cell line 7721786(C)
|
|
|
|
| Obiri N etal (1993) J Clin Invet 91, 88-93
|
|
|
|
| rabbit kidney cells 19176525(C)
|
|
|
|
| BLM-deficient fibroblasts 16199871(C)
|
|
|
|
| Gaymes, T. J., P. S. North, N. Brady, I. D. Hickson, G. J. Mufti, and F. V.
Rassool. 2002. Increased error-prone non-homologous DNA end-joining—a proposed mechanism of chromosomal instability in Bloom’s syndrome. Oncogene 21:2525–2533. PMID:11971187
|
|
|
|
| PT67 CRL-12284 mouse fibroblast adherent embryo; amphotropic retroviral packaging line
|
|
|
|
| Py-4-1 mouse endothelial cells (a generous gift of Victoria Bautch, University of
North Carolina, Chapel Hill, NC) 11029289(C)
|
|
|
|
| A Tgfb1-resistant mutant of MV1LU which lacks TgfbR1 1333888(C)
|
|
|
|
| Mv1Lu / R1B-L17 is a derivative cell line defective in the TgfbR1 receptor that has a high
transfection efficiency and responds to TGFb on transfection of TgfbR1. 10197981(C)
|
|
|
|
| Wrana, J.L., L. Attisano, R. Wieser, F. Ventura, and J. Massague´. 1994. Mechanism of activation of the TGF-beta receptor. Nature 370: 341–347.
|
|
|
|
| derived from Mv1Lu - defective in TgfbR1 10197981(C)
|
|
|
|
| mink lung epithelial cells 7791754(C)
|
|
|
|
| R-l B cell line was derived from Mvl Lu by chemical mutagenesis and is resistant to TGFB owing to a deficiency in the type I receptor (Boyd and Massagub, 1989; Wrana et al., 1992).
|
|
|
|
| a mutant of Mv1Lu cells used in 11094085 without explanation
|
|
|
|
| Raji CCL-86 human lymphoblast suspension B lymphocyte; Burkitt’s lymphoma
|
|
|
|
| an Epstein-Barr virus-infected B-cell line 1631559(C)
|
|
|
|
| expresses IL2Rg mRNA 1631559(D)
|
|
|
|
| does not bind IL13 7721786(D)
|
|
|
|
| Ramos (RA 1) CRL-1596 human lymphoblast suspension B lymphocyte; Burkitt’s lymphoma (American)
|
|
|
|
| Burkitt lymphoma cell line
|
|
|
|
| Katz, P., G. Whalen, and J. H. Kehrl. 1994. Differential expression of a novel
protein kinase in human B lymphocytes: preferential localization in the
germinal center. J. Biol. Chem. 269:16802–16809.
|
|
|
|
| Rat1-R12 CRL-2210 rat epithelial adherent connective tissue
|
|
|
|
| Rat1-5R: defective in Nemo
|
|
|
|
| Yamaoka, S., Courtois, G., Bessia, C., Whiteside, S. T., Weil, R., Agou, F., Kirk, H. E., Kay, R. J., and Israel, A. (1998) Cell 93, 1231–1240
|
|
|
|
| used without explanation in 10835426 (as Rat-1a) and 16027121 (as Rat1A)
|
|
|
|
| Rat2 CRL-1764 rat fibroblast adherent fetus
|
|
|
|
| RAW264.7 TIB-71 mouse adherent monocyte; macrophage; AMLV-induced tumor
|
|
|
|
| Phd1-gene mRNA 19762779(D)
|
|
|
|
| Phd2-gene mRNA 19762779(D)
|
|
|
|
| not Phd3-gene mRNA 19762779(D)
|
|
|
|
| mouse macrophage line 11323673(C)
|
|
|
|
| RBL-2H3 CRL-2256 rat fibroblast adherent peripheral blood; basophil; leukemia
|
|
|
|
| 11686307(D): RBL-2H3 cells produce only MHC-A (Choi et al., 1996)
|
|
|
|
| Choi, O. H., Park, C.-S., Itoh, K., Adelstein, R. S. and Beaven, M. A. (1996). Cloning of the cDNA encoding rat myosin heavy chain-A and evidence for the absence of myosin heavy chain-B in cultured rat mast (RBL-2H3) cells. J. Muscle Res. Cell Motil. 17, 69-77.
|
|
|
|
| rabbit normal cornea cells
|
|
|
|
| used in 16630891 as a "neuroblastoma" no other info given
|
|
|
|
| rhabdomyosarcoma-derived line 6434688(C)
|
|
|
|
| rat embryo fibroblast line 8657134(C)
|
|
|
|
| "normal rat fibroblasts" 10480934(C)
|
|
|
|
| a non-T non-B line 2261637(C)
|
|
|
|
| expresses mRNA for Gp130 but not IL6R 2261637-Fig-4
|
|
|
|
| A rat cell line (RGM-1) was established from normal gastric mucosa 15326485(C)
|
|
|
|
| Kobayashi I, Kawano S, Tsuji S, Matsui H, Nakama A, Sawaoka H, Masuda E, Takei Y, Nagano K, Fusamoto H, Ohno T, Fukutomi H and Kamada T. (1996). In Vitro Cell
Dev. Biol. Anim., 32, 259–261.
|
|
|
|
| a TGF-beta type I receptor expressing clone of highly transfectable MV1LU-L17 cells 12621041
|
|
|
|
| "epithelial cells" 16959574(C)
|
|
|
|
| "rabbit kidney cells" 7512734(C)
|
|
|
|
| RK13 CCL-37 rabbit kidney epithelial adherent
|
|
|
|
| CRL-2577 human epithelial adherent colon; carcinoma; control for RKO-E6 and RKO-AS45-1 cells
|
|
|
|
| 909698 BRAF p.V600E c.1799T>A Heterozygous
|
|
|
|
| 909698 NF1 p.N2341fs*5 c.7022delA Heterozygous
|
|
|
|
| 909698 PIK3CA p.H1047R c.3140A>G Heterozygous
|
|
|
|
| In RKO cells, b-catenin does not localize to the plasma membrane, whereas in other cell types, such as HEK293T cells, it resides with a relatively long half-life at the inner surface of the plasma membrane. Thus, in the absence of membrane-associated b-catenin, total cellular levels of b-catenin in RKO cells are very low, which allows study of cytoplasmic and nuclear b-catenin stability in re- sponse to experimental perturbation. 17510365(C)
|
|
|
|
| an IL6-independent human myeloma cell line which harbors a Ras mutation 10023775(C)
|
|
|
|
| express TLR1, TLR6, TLR7, TLR9, TLR10, Cd180 but not TLR2, TLR3, TLR4, TLR5, TLR8 mRNA [RTPCR] 15294971-Fig-1
|
|
|
|
| a MM cell line with a translocation involving the c-MAF gene (Chesi et al., 1998). 19446321(C)
|
|
|
|
| Chesi, M., Bergsagel, P.L., Shonukan, O.O., Martelli, M.L., Brents, L.A., Chen, T., Schrock, E., Ried, T., and Kuehl, W.M. (1998). Frequent dysregulation of the c- maf proto-oncogene at 16q23 by translocation to an Ig locus in multiple myeloma. Blood 91, 4457–4463.
|
|
|
|
| express normal levels of Mtor Pten Akt1 S6k1 19946321(D)
|
|
|
|
| express higher than normal levels of Deptor 19946321(D)
|
|
|
|
| CDKN2C p.0? c.1_507del507 Homozygous
EGFR p.T751I c.2252C>T Heterozygous
KRAS p.G12A c.35G>C Heterozygous
TP53 p.E285K c.853G>A Homozygous
|
|
|
|
| human B lymphoblastoid EBV-transformed cell line 2504877(C)
|
|
|
|
| 16.Jensen, J.R .,T T.Sand, and H .L.Spiegelberg. 1984. Generation of IgE-binding and IgG-binding factors from human lymphoblastoid cell lines. Immunology.53:1.
|
|
|
|
| human B-cell follicular lymphoma cell line 15670770(C)
|
|
|
|
| RT4-D6P2T CRL-2768 rat neuronal adherent Schwann cell; schwannoma
|
|
|
|
| a rat schwannoma cell line in which merlin can be expressed from a stably transfected ectopic, doxycycline-inducible (TetON) construct (22). 16537381(C)
|
|
|
|
| 24. Shaw, R. J., McClatchey, A. I. & Jacks, T. (1998) J. Biol . Chem. 273, 7757–7764.
|
|
|
|
| used in 12640114 without explanation
|
|
|
|
| Human Renal Hypernephroma Adherent
|
|
|
|
| CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.0? c.1_522del522 Homozygous
PTEN p.? c.1_164del164 Homozygous
TP53 p.R175H c.524G>A Homozygous
|
|
|
|
| SAM-19 cells were derived from the autonomously growing mouse CTL/rat thymoma hybrid PC60 (25) that cannot be activated by IL-2 on its own to express perforin (26). We noticed a weak IL-2 response of the perforin gene in a subline designated SA. Further analysis indicated that the SA line, but not the other lines, contained cells constitutively expressing the mouse IL-2Rb chain. Constitutive expression of mouse IL-2Rg was equivalent in all lines. After cloning these cells at the single-cell level, one clone, SAM-19, was obtained that expressed IL-2Rb mRNA at levels equivalent to those in the CTL effector–like CTLL-2 line. Neither resting nor IL-2–stimulated cells expressed detectable levels of mouse IL-2Ra protein or mRNA.
|
|
|
|
| 25. Conzelmann, A., P. Corthesy, M. Cianfriglia, A. Silva, and M. Nabholz. 1982. Hybrids between rat lymphoma and mouse T cells with inducible cytolytic activity. Nature. 298: 170–172.
|
|
|
|
| 26. Garcia-Sanz, J.A., and E.R. Podack. 1993. Regulation of perforin gene expression in a T cell hybrid with inducible cy- tolytic activity. Eur. J. Immunol. 23:1877–1883.
|
|
|
|
| "have a deletion of the type I IFN locus" 9660935(C)
|
|
|
|
| Miyakoshi, J., Dobler, K.D., Allalunis-Turner, J., McKean, J.D., Petruk, K., Allen, P.B., Aronyk, K.N., Weir, B., Huyser-Wierenga, D., Fulton, D., Urtasum, R.C., and Day, R.S., III (1990). Absence of IFNA and IFNB genes from human malignant glioma cell lines and lack of correlation with cellular sensitivity to interferons. Cancer Res. 50, 278–283.
|
|
|
|
| Saos-2 HTB-85 human epithelial adherent bone; osteosarcoma
|
|
|
|
| does not express endogenous p53 15073170(C)
|
|
|
|
| p53-negative [RTPCR] 16676006(D)
|
|
|
|
| does not express Pten mRNA 9187108(D)
|
|
|
|
| pRb-neg p53-neg 9724636(C)
|
|
|
|
| a human B cell line 8995252(C)
|
|
|
|
| a non-invasive human melanoma 11278329(C)
|
|
|
|
| human keratinocytes, epithelial cell line 11716761(C)
|
|
|
|
| Sf21 (officially called IPLB-Sf21AE) is a continuous cell line developed from ovaries of the Fall Army worm, Spodoptera frugiperda, a moth species that is an agricultural pest on corn and other grass species. It was originally developed in the United States at the Henry A. Wallace Beltsville Agricultural Research Center. Sf9 is a substrain (clone) of these cells that was isolated from Sf21 by researchers at Texas A&M University. [Wikipedia]
|
|
|
|
| part of NCI60 not in ATCC
|
|
|
|
| info from data sheet provided by NCI-DTP
|
|
|
|
| human CNS gloioblastoma multiform adherent
|
|
|
|
| source: Dr. M. L. Rosenblum
|
|
|
|
| reference: Rutka, J., Giblin. J., Dougherty, D., Liu, H., McCulloch, J., Bell, C., Stern, R., Wilson, B., Rosenblum, M.; Establishment and Characterization of Five Cell Lines Derived from Human Malignant Gliomas; Acta Neuropathologica 75:92-103, 1987. CNS Lines acquired fro Dr. Rosenblum at UCSF, SF-268 and SF-295 were called a high grade astrocytoma and glioblastoma - which shows that sometimes it can be difficult to describe a cell line with one label or another - sometimes patient tumors can exhibit evidence of mixed grades.
|
|
|
|
| CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.0? c.1_522del522 Homozygous
PTEN p.R233* c.697C>T Homozygous
TP53 p.R248Q c.743G>A Homozygous
|
|
|
|
| Sf9 CRL-1711 armyworm, epithelial adherent fall ovary
|
|
|
|
| used without explanation in 16919458(C)
|
|
|
|
| SH-SY5Y CRL-2266 human bone marrow (metastasis); (brain primary) neuroblastoma epithelial adherent
|
|
|
|
| do not express Ntrk2 mRNA [Northern] 1710174-Fig-1b
|
|
|
|
| do not express Ctnnb1 in nuclear fraction [WB] 16007074-Fig-S3
|
|
|
|
| used without explanation in 15242646(C)
|
|
|
|
| used without explanation in 16163388(C)
|
|
|
|
| osteosarcoma; multipotential sarcoma
|
|
|
|
| There is amplification of the gene which encodes the p53 associated protein, MDM2.
SJSA-1 cells also exhibit a 15 fold amplification of the gli proto-oncogene.
|
|
|
|
| 22379: Oliner JD, et al. Amplification of a gene encoding a p53-associated protein in human sarcomas. Nature 358: 80-83, 1992. PubMed: 1614537
23076: Roberts WM, et al. Amplification of the gli gene in childhood sarcomas. Cancer Res. 49: 5407-5413, 1989. PubMed: 2766305
|
|
|
|
| SK-BR-3 HTB-30 human epithelial adherent pleural effusion (metastasis); adenocarcinoma (colon primary)
|
|
|
|
| derived from ER-negative breast cancer tissue 16596714(C)
|
|
|
|
| does not express Huwe1 protein 15989956(D)
|
|
|
|
| SK-HEP-1 HTB-52 human epithelial adherent ascites (metastasis); adenocarcinoma (liver primary)
|
|
|
|
| a liver cell line that contains Ca2+ signaling machinery in both the cytoplasm and the nucleus (20). 18073207(C)
|
|
|
|
| 20. Echevarria,W.,Leite,M.F.,Guerra,M.T.,Zipfel,W.R.,and Nathanson, M. H. (2003) Nat. Cell Biol. 5, 440 – 446
|
|
|
|
| SK-LMS-1 HTB-88 human fibroblast adherent vulva; leiomyosarcoma
|
|
|
|
| SK-LMS-1 is a human leiomyosar- coma cell line that overexpresses Met 1134526(C)
|
|
|
|
| SK-MEL-2 HTB-68 human skin (metastasis); malignant melanoma (skin polygonal adherent primary)
|
|
|
|
| NRAS p.Q61R c.182A>G Homozygous
TP53 p.G245S c.733G>A Heterozygous
|
|
|
|
| human adherent stellate from skin malignant melanoma
|
|
|
|
| BRAF p.V600E c.1799T>A Heterozygous
CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.0? c.1_522del522 Homozygous
STK11 p.? c.1_290del290 Homozygous
|
|
|
|
| SK-MEL-28 HTB-72 human skin; malignant melanoma polygonal adherent
|
|
|
|
| BRAF p.V600E c.1799T>A Homozygous
EGFR p.P753S c.2257C>T Homozygous
TP53 p.L145R c.434_435TG>GT Homozygous
|
|
|
|
| contains Braf(V600E) 19274086(C)
|
|
|
|
| contains Lkb1 protein 19187764-Fig-1a,3b
|
|
|
|
| SK-MEL-31 HTB-73 human epithelial adherent skin; malignant melanoma
|
|
|
|
| not in Casmic database but listed as being from the same individual as SKMEL2
|
|
|
|
| contains LKb1 protein 19187764(C)
|
|
|
|
| contains Lkb1 protein 19187764-Fig-1a
|
|
|
|
| a human melanoma line with far fewer NGF receptors 6093111(C)
|
|
|
|
| SK-MES used in 8272873 without explanation
|
|
|
|
| SK-MG-4 an NK-like cell line 2261637(C)
|
|
|
|
| expresses mRNA for IL6R and GP130 2261637-Fig-4
|
|
|
|
| SK-N-DZ CRL-2149 human epithelial adherent bone marrow (metastasis); embryonal neuro-blastoma (brain/neuroblast primary)
|
|
|
|
| SK-N-MC HTB-10 human epithelial adherent supraorbital area (metastasis); neuroepithelioma (brain primary)
|
|
|
|
| express Gp130 and LifR 16774741(C)
|
|
|
|
| K.J. Kallen, J. Grotzinger, E. Lelievre, P. Vollmer, D. Aasland, C. Renne, J. Mullberg, K.H. Myer zum Buschenfelde, H. Gascan, S. Rose-John, Receptor recognition sites of cytokines are organized as exchangeable modules. Transfer of the leukemia inhibitory factor receptor-binding site from ciliary neurotrophic factor to interleukin-6, J. Biol. Chem. 274 (1999) 11859–11867.
|
|
|
|
| adherent human neuroblastoma derived from bone marrow metastasis
|
|
|
|
| express endogenous OpRd1 and Arrb1 16325578(C)
|
|
|
|
| Yu, V.C., Richards, M.L., and Sadee, W. (1986). A human neuroblastomcell line expresses mu and delta opioid receptor sites. J. Biol. Chem. 261065–1070. 3003051
|
|
|
|
| SK-OV-3 HTB-77 human epithelial adherent ascites (metastasis); adenocarcinoma (ovaryprimary)
|
|
|
|
| CDKN2A p.? c.1_457del457 Homozygous
PIK3CA p.H1047R c.3140A>G Heterozygous
TP53 p.S90fs*33 c.267delC Homozygous
|
|
|
|
| SKOV3.ip1 used in 17693255 without explanation
|
|
|
|
| human Lymphoma or Leukemia cell line 16603397(C)
|
|
|
|
| SKW-3 (human, peripheral blood, leukemia, T cell)
SPECIES: human
TISSUE/ORGAN: peripheral blood
TUMOR: leukemia, T cell
DSMZ catalog code: ACC 53
Morphology: single, round cells in suspension
Description: human T cell leukemia established from the peripheral blood of a 61-year-old man with T cell chronic lymphocytic leukemia (CLL) in 1977 confirmed as human with IEF of MDH, NP
Viruses: ELISA: reverse transcriptase negative; PCR: EBV-, HBV-, HCV-, HHV-8-, HIV-, HTLV-I/II-
Depositor: Dr. J. Minowada, Fujisaki Cell Center, Okayama, Japan
General Restrictions
J Immunol 1979;123:1133-1140 - PMID: 313946
|
|
|
|
| SL29 CRL-1590 chicken fibroblast adherent embryo
|
|
|
|
| Trypsinization of a decapitated 11-day leghorn embryo. The cells have a doubling potential of 35 population doublings.
|
|
|
|
| testicular cell line derived from immature Sertoli cells by targeted oncogenesis (20) 10854429(C)
|
|
|
|
| 20. Dutertre, M., Rey, R., Porteu, A., Josso, N., and Picard, J. Y. (1997) Mol. Cell.
Endocrinol. 136, 57– 65
|
|
|
|
| a hepatoma cell line 12000790(C)
|
|
|
|
| Satoh, S., Daigo, Y., Furukawa, Y., Kato, T., Miwa, N., Nishi- waki, T., Kawasoe, T., Ishiguro, H., Fujita, M., Tokino, T., et al. 2000. AXIN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1. Nat. Genet. 24: 245–250.
|
|
|
|
| Cdkn2a p.0? c.1_471del471 homozygous
|
|
|
|
| Cdkn2a(p14) p.0? c.1_471del471 homozygous
|
|
|
|
| Tp53 p.A161T c.481G>A homozygous
|
|
|
|
| a hepatocellular carcinoma 19131971(C)
|
|
|
|
| Satoh S, Daigo Y, Furukawa Y, Kato T, Miwa N, Nishiwaki T, Kawasoe T, Ishiguro H, Fujita M, Tokino T, Sasaki Y, Imaoka S, Murata M, Shimano T, Yamaoka Y, Nakamura Y (2000) AXIN1 mutations in hepatocellular carcinomas, and growth suppression in cancer cells by virus-mediated transfer of AXIN1. Nat Genet 24: 245–250
|
|
|
|
| 10202139(C): 3Y1 Rat Fibroblasts transformed with vSrc
|
|
|
|
| from Japanese Cancer Research Resources Bank
|
|
|
|
| 16959574(C): bone marrow stroma cells
|
|
|
|
| a pre-B acute lymphoblastic leukaemia cell line 19131971(C)
|
|
|
|
| contains an Axin1 inframe deletion that removes aa 373-418 19131971 (data not shown)
|
|
|
|
| human keratinocyte line which expresses IL4 receptors and produce IL6 in response to IL4 stimulation 8621475(C)
|
|
|
|
| 13. Taylor-Papadimitriou, J., Purkis, P., Lane, E. B., McKay, I. A., and Chang, S.
E. (1982) Cell Differ. 11, 169–180
|
|
|
|
| human anaplastic thyroid carcinoma cells 9335507(C)
|
|
|
|
| SW48 CCL-231 human colon; colorectal adenocarcinoma epithelial adherent
|
|
|
|
| Ctnnb1 p.S33Y c.98C>A heterozygous
|
|
|
|
| EgfR p.G719S c.2155G>A heterozygous
|
|
|
|
| Fbxw7 p.S668fs*39 c.2001gelG heterozygous
|
|
|
|
| SW48 cells contained stabilized beta-catenin with a mutation in the NH2-terminus (11)
|
|
|
|
| 11. V. Korinek et al., Science 275, 1784 (1997); P. J. Morin et al., ibid., p. 1787; B. Rubinfeld et al., ibid., p. 1790.
|
|
|
|
| SW480 CCL-228 human colon; colorectal adenocarcinoma epithelial adherent
|
|
|
|
| In SW480 cells only a COOH-terminally truncated APC protein is produced that lacks all SAMP and six 20–amino acid repeats. As a consequence, beta-catenin accumulates in the cytoplasm and nucleus; introduction of wild-type APC causes beta-catenin degradation (9, 11). 9554852(C)
|
|
|
|
| 9. S. Munemitsu, I. Albert, B. Souza, B. Rubinfeld, P. Polakis, Proc. Natl. Acad. Sci. U.S.A. 92, 3046 (1995); B. Rubinfeld et al., Science 272, 1023 (1996).
|
|
|
|
| 11. V. Korinek et al., Science 275, 1784 (1997); P. J. Morin et al., ibid., p. 1787; B. Rubinfeld et al., ibid., p. 1790.
|
|
|
|
| a colorectal adenocarcinoma cell line lacking the wild-type p53 gene 11030628(C)
|
|
|
|
| ATCC: There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution and a C -> T mutation in codon 309 resulting in a Pro -> Ser substitution. [22870] [23322]
|
|
|
|
| 22870: Weiss J, et al. Mutation and expression of the p53 gene in malignant melanoma cell lines. Int. J. Cancer 54: 693-699, 1993. PubMed: 8514460
|
|
|
|
| 23322: Rodrigues NR, et al. p53 mutations in colorectal cancer. Proc. Natl. Acad. Sci. USA 87: 7555-7559, 1990. PubMed: 1699228
|
|
|
|
| express Ctnnb1 in nuclear fraction [WB] 16007074-Fig-S3
|
|
|
|
| a Smad4 deficient cell line 11265759(C)
|
|
|
|
| human B cell line SKW 6.4 16046471(C)
|
|
|
|
| colon carcinoma 10197981(C)
|
|
|
|
| 16. M. van de Wetering, M. Oosterwegel, K. van Norren, H. Clevers, EMBO J. 12, 3847 (1993); V. Korinek and N. Barker, unpublished data.
|
|
|
|
| APC p.Q1338* c.4012C>T Homozygous
KRAS p.G12V c.35G>T Homozygous
MAP2K4 p.? c.219_891del673 Homozygous
SMAD4 p.? c.955+5G>C Homozygous
TP53 p.P309S c.925C>T Homozygous
TP53 p.R273H c.818G>A Homozygous
|
|
|
|
| 3T3-Swiss-albino CCL-92 mouse fibroblast adherent embryo; feeder layer cell
|
|
|
|
| SYF<Src~null><Yes~null><Fyn~null>
|
|
|
|
| mouse embryo fibroblast deficient for Src, Yes, and Fyn immortalized with SV40 large T antigen
|
|
|
|
| The Tax-immortalized T cell line, T1, was derived from infection of primary human thymocytes with a Herpes- virus saimiri vector encoding the HTLV-1 pX-region, as previously described (Grassmann et al., 1989). Analysis of the growth characteristics of T1 cells in the absence of IL-2 demonstrated that these cells maintain good viability throughout the first 5 days of culture without IL-2; it is not until later that the cell numbers rapidly decrease. 15735688(C)
|
|
|
|
| Grassmann R, Dengler C, Muller-Fleckenstein I, Fleckenstein B, McGuire K, Dokhelar MC, Sodroski JG and Haseltine WA. (1989). Proc. Natl. Acad. Sci. USA, 86, 3351–3355.
|
|
|
|
| T24 HTB-4 human epithelial adherent urinary bladder; transitional cell carcinoma
|
|
|
|
| HRAS p.G12V c.35G>T Homozygous
TP53 p.Y126* c.378C>G Homozygous
|
|
|
|
| expresses mRNA for Gp130 but not IL6R 2261637-Fig-4
|
|
|
|
| T24 and T24T human bladder cancer cell lines with nonmetastatic and metastatic phenotypes, respectively, and their growth conditions and doubling time of 30 h have been described previously (3, 4). 11861368(C)
|
|
|
|
| 3. Seraj, M. J., Harding, M. A., Gildea, J. J., Gulding, K. M., and Theodorescu, D. The
relationship of BRMS1 and RhoGDI2 gene expression to metastatic potential in
lineage related human bladder cancer cell lines. Clin. Exp. Metastasis, 18: 519 –525,
2000.
4. Gildea, J. J., Golden, W. L., Harding, M. A., and Theodorescu, D. Genetic and
phenotypic changes associated with the acquisition of tumorigenicity in human
bladder cancer. Genes Chromosomes Cancer, 27: 252–263, 2000.
|
|
|
|
| T-47D HTB-133 human epithelial adherent pleural effusion (metastasis); ductal carcinoma (mammary gland primary)
|
|
|
|
| PIK3CA p.H1047R c.3140A>G Heterozygous
TP53 p.L194F c.580C>T Homozygous
|
|
|
|
| expresses high levels of Huwe1 protein 15989956(D)
|
|
|
|
| express high levels of Pin1 11432833-Fig-2d
|
|
|
|
| ATCC CRL-1690 human glioblastoma multiforme adherent
|
|
|
|
| a T-cell line from WKA/Hok rats 8266076(C)
|
|
|
|
| M. Tateno etal J Exp Med 159, 1105 (1984)
|
|
|
|
| does not express IL2Rg [FACS] 8266076-Fig-1
|
|
|
|
| TARTMG: TART1<IL2Rg-null><xIL2Rg>
|
|
|
|
| human Ewing’s sarcoma 16380506(C)
|
|
|
|
| "TE671 is a human rhabdomyosarcoma cell line from American Type Culture Collection" 9020886(C)
|
|
|
|
| possibly: RD CCL-136 human spindle and large adherent muscle; rhabdomyosarcoma
|
|
|
|
| megakaryocytic cell line 11331873(C)
|
|
|
|
| TF-1 CRL-2003 human lymphoblast suspension bone marrow, erythroblast; model for cytokine(IL-3) signaling pathways and myeloid progenitor cell differentiation
|
|
|
|
| TF1.J61 premyeloid 7721786(C)
|
|
|
|
| used without explanation in 7781605
|
|
|
|
| THLE-2 CRL-2706 human epithelial adherent liver, left lobe; SV40 transformed
|
|
|
|
| human macrophage-like cell line
|
|
|
|
| does not express IL2Rg mRNA 1631559(D)
|
|
|
|
| THP-1 TIB-202 human monocyte; acute monocytic leukemia monocyte suspension
|
|
|
|
| CR3; complement (C3) receptor
|
|
|
|
| HLA A2, A9, B5, DRw1, DRw2 antigens
|
|
|
|
| Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
vWA: 16
|
|
|
|
| CAMR Centre for Applied Microbiology & Research (ECACC, Salisbury, Wiltshire)
|
|
|
|
| Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for alpha-naphthyl butyrate esterase produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Con A, show increased CO2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.
|
|
|
|
| Refs: Int J Cancer 1980;26:171; Cancer Res 1982;42:1530; J Immunol 1983;131:1882
|
|
|
|
| Availability in cell line catalogues: ATCC TIB 202; DSMZ ACC 16; ICLC HTL97014
|
|
|
|
| Genomic Mutations: [COSMIC/Cosmic]
|
|
|
|
| CDKN2A p.M1_*157del c.1_471del471 Confirmed somatic variant Homozygous
|
|
|
|
| Cdkn2a (p16-Ink4): OFF mutation AAa 1-21 deletion (inhibitor of proliferation)
|
|
|
|
| NRAS p.G12D c.35G>A Confirmed somatic variant Heterozygous
|
|
|
|
| Nras: G12D Activiating mutation (activator of proliferation)
|
|
|
|
| TP53 p.R174fs*3 c.520_545del26 Confirmed somatic variant Homozygous
|
|
|
|
| OFF mutation R174 deletion causing frameshift (inhibitor of proliferation)
|
|
|
|
| Genes with no mutations detected so far:
|
|
|
|
| APC, BRAF, CBFB, CDH1, CTNNB1, CYLD, EGFR, FBXW7, FGFR3, FLCN, FLT3, GNAS, HRAS, JAK2, KIT, KRAS, MAP2K4, MLH1, MSH2, MSH6, MYC, NF1, NF2, NOTCH1, NPM1, NTRK3, PALB2, PIK3C2A, PIK3CA, PTCH, PTEN, RB1, RET, RUNX1, SMAD4, SMO, SOCS1, STK11, SUFU, TSC1, TSC2, VHL, WT1
|
|
|
|
| http://www.functionalglycomics.org/glycomics/publicdata/glycoprofiling.jsp?species=cellLines&cellType=Human%20THP-1
|
|
|
|
| Other characteristics [10223614]
|
|
|
|
| Positive for nonspecific esterase (alpha-naphtyl-butyrate) 10223614(C)
|
|
|
|
| Normal human LDH isozyme patterns 10223614(C)
|
|
|
|
| does not form rosettes with neuraminidase SRBC (assay for T cells) 10223614(C)
|
|
|
|
| FcgR1a (Cd64) positive 10223614(C)
|
|
|
|
| FcgR2b (Cd32) positive 10223614(C)
|
|
|
|
| FcgR3a and FcgR3b (Cd16) negative 10223614(C)
|
|
|
|
| Positive for HLA-DR surface expression 10223614(C)
|
|
|
|
| Negative for Cd1a 10223614(C)
|
|
|
|
| Positive for CR3 (Mac1) surface expression 10223614(C)
|
|
|
|
| Positive for CR4 (Cd11c) surface expression 10223614(C)
|
|
|
|
| Capable of reducing Nitroblue terazolium 10223614(C)
|
|
|
|
| Phagocytizes Latex but not SRBC in HI-FBS 10223614(C)
|
|
|
|
| TnfR1-positive [RTPCR] 19854138(D)
|
|
|
|
| TnfR2-negative [RTPCR] 19854138(D)
|
|
|
|
| THP1 grown in RPMI/HI-FCS + Csf2 + IL4 for 5 days
|
|
|
|
| THP1 grown in RPMI + IL4 + Csf2 + Tnf + Ionomycin for 1-3 days
|
|
|
|
| express TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR9, TLR10, Cd180 but not TLR8 mRNA [RTPCR] 15294971-Fig-1
|
|
|
|
| used in 16007074 as "fibroblasts"
|
|
|
|
| do not express Ctnnb1 in nuclear fraction [WB] 16007074-Fig-S3
|
|
|
|
| The TIG3 strain of HDFs (primary human diploid fibroblasts) that had been rendered sensitive to ecotropic murine retroviruses by expression of the mouse basic amino acid transporter (McConnell et al., 1998) 9724636(C)
|
|
|
|
| McConnell,B.B., Starborg,M., Brookes,S. and Peters,G. (1998) Inhibitors of cyclin-dependent kinases induce features of replicative senescence in early passage human diploid fibroblasts. Curr. Biol., 8, 351–354.
|
|
|
|
| used in 12438239 TRKE-2 rat kidney epithelial cell; Ref. 37
|
|
|
|
| 37. Walker, C., and Ginsler, J. Development of a quantitative in vitro transformation
assay for kidney epithelial cells. Carcinogenesis (Lond.), 13: 25–32, 1992.
|
|
|
|
| dog kidney epithelial cells 8264620(C)
|
|
|
|
| TS1 proliferates in response to IL4 and IL9
|
|
|
|
| TS1αβ is a murine T-cell line stably transfected with the α- and β-chains of the human IL-2 receptor (Pitton et al., 1993), and can be maintained independently in the presence of IL-2, IL-4 or IL-9. Cells were cultured in RPMI-1640 (BioWhittaker, Walkersville, MD) supplemented
with 5% heat-inactivated fetal calf serum (FCS; Gibco-BRL, Gaithersburg, MD), 2 mM glutamine, 10 mM HEPES, 0.55 mM arginine, 0.24 mM asparagine, 50 µM 2-ME and 5 ng/ml of recombinant IL-2 (rIL-2) or 60 U/ ml of IL-4.
|
|
|
|
| Pitton,C., Rebollo,A., Van Snick,J., Theze,J. and Garcıa,A. (1993) High affinity and intermediate affinity forms of the human IL-2R, expressed in an IL-9-dependent murine T cell line deliver proliferative signals via differences in their transduction pathways. Cytokine, 5, 362–371.
|
|
|
|
| a clone of HEK293 cells stably expressing SV40 TAg 11022047(C)
|
|
|
|
| The cell line used in this study is the prostate carcinoma cell line TSU-pr1 (Iizumi et al., 1987). This cell line was derived from human prostatic adenocarcinoma metastatic to lymph node (kindly provided by A. Passaniti, University of Maryland National Institute of Health, Baltimore).
|
|
|
|
| Iizumi, T., Yazaki, T., Kanoh, S., Kondo, I. and Koiso, K. (1987). Establishment of a new prostatic carcinoma cell line (TSU-pr1). J. Urol. 137, 1304-1306.
|
|
|
|
| fibrosarcoma cells lacking Tyk2 9794795(C)
|
|
|
|
| a mutant derived from 2C4 cells that lack Tyk2 mRNA and protein 7537214(C)
|
|
|
|
| does not express Tyk22 protein 7537214-Fig-2a
|
|
|
|
| expresses Jak2 protein 7537214-Fig-2a
|
|
|
|
| expresses Jak1 protein 7537214-Fig-2b
|
|
|
|
| human glioblastoma astrocytoma
|
|
|
|
| CDKN2A p.0? c.1_471del471 Homozygous
CDKN2a(p14) p.0? c.1_522del522 Homozygous
PTEN p.? c.1026+1G>T Homozygous
TP53 p.R213Q c.638G>A Homozygous
|
|
|
|
| "U-251 human glioblastoma cells were obtained from the National Cancer Institute
Frederick Cancer DCT Tumor Repository" 16390869(C)
|
|
|
|
| multiple myeloma 9374471(C)
|
|
|
|
| U266B1 TIB-196 human B lymphoblast; plasmacytoma; myeloma lymphoblast, suspension
|
|
|
|
| expresses mRNA for Gp130 2261637-Fig-4
|
|
|
|
| expresses mRNA for IL6R 2261637-Fig-4 3136546-Fig-3
|
|
|
|
| expresses IL6R protein 2788034-Fig-2
|
|
|
|
| contain IL6 binding sites 3136546-Fig-3
|
|
|
|
| Irf9-null cells cells received from G Stark 17923090(C)
|
|
|
|
| U2OS HTB-96 human epithelial adherent bone; osteosarcoma
|
|
|
|
| p53-positive [RTPCR] 16676006(D)
|
|
|
|
| TnfR1-positive [RTPCR] 19854138(D)
|
|
|
|
| TnfR2-negative [RTPCR] 19854138(D)
|
|
|
|
| wild-type for both p53 and pRb (Diller et al., 1990) 9724636(C)
|
|
|
|
| Diller,L. et al. (1990) p53 functions as a cell cycle control point in osteosarcoma. Mol. Cell. Biol., 10, 5772–5781.
|
|
|
|
| Cosmic mutations: none found
|
|
|
|
| "human astrocytoma" 11179315(C)
|
|
|
|
| a Stat1-deficient human cell line 1048577(C) derived from 2fTGH cells
|
|
|
|
| a somatic mutant human fibrosarcoma cell line lacking both Jak1 and Jak3 10825200(C)
|
|
|
|
| 9392798: Liu, K. D., S. L. Gaffen, M. A. Goldsmith, and W. C. Greene. 1997. Janus kinases in interleukin-2 mediated signaling: JAK1 and JAK3 are differentially regulated by tyrosine phosphorylation. Curr. Biol. 7:817–826.
|
|
|
|
| a somatic mutant derivative of the 2FTGH human fibrosarcoma cell line that lacks endogenous JAK1 [41] and apparently fails to express JAK3 9382798(C)
|
|
|
|
| 41. Müller M, Briscoe J, Laxton C, Guschin D, Ziemiecki A, SilvennoinenO, et al.: The protein tyrosine kinase JAK1 complements defects in interferon-alpha/beta and -gamma signal transduction. Nature 1993, 366:129-166.
|
|
|
|
| does not express Jak1 protein 7537214-Fig-2b
|
|
|
|
| expresses Jak2 protein 7537214-Fig-2a
|
|
|
|
| expresses Tyk2 protein 7537214-Fig-2a
|
|
|
|
| stable derivative U4A/Jak1: U4A<Jak1-null><xJak1>
|
|
|
|
| used in 11163210 without description
|
|
|
|
| we used U4A/Jak1 cells, which express endogenous Smurf2 and in which SnoN concentration decreases in a proteasome-dependent manner in response to TGF-β signalling.
|
|
|
|
| IfnaR2-null cells received from G Stark 17923090(C)
|
|
|
|
| Stat2-null cells received from G Stark 17923090(C)
|
|
|
|
| Synonyms: U87 U-87 U-87 MG
|
|
|
|
| U87MG HTB-14 human epithelial adherent brain; glioblastoma; astrocytoma
|
|
|
|
| 89081402 U-87 MG Human glioblastoma astrocytoma
|
|
|
|
| http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=89081402&collection=ecacc_gc
|
|
|
|
| human astrocytoma 11179315(C)
|
|
|
|
| 687590 CDKN2A p.M1_*157del c.1_471del471 Homozygous
|
|
|
|
| 687590 PTEN p.? c.209+1G>T Homozygous
|
|
|
|
| Express abnormally low levels of Deptor 19446321(D)
|
|
|
|
| U937 CRL-1593.2 human monocyte suspension macrophage; histiocytic lymphoma
|
|
|
|
| macrophage-like 15953029(C)
|
|
|
|
| does not bind IL13 7721786(D)
|
|
|
|
| expresses mRNA for IL6R 3136546-Fig-3
|
|
|
|
| contain IL6 binding sites 3136546-Fig-3
|
|
|
|
| MSH6 p.P1082P c.3246G>T Heterozygous 9510473
|
|
|
|
| PTEN p.G129fs*51 c.387_388insCGCC 10966170
|
|
|
|
| BRAF p.V600E c.1799T>A Heterozygous
|
|
|
|
| contains Lkb1 protein 19187764-Fig-1a,6d
|
|
|
|
| BRAF p.V600E c.1799T>A Homozygous
CDKN2A p.M1_*157del c.1_471del471 Homozygous
PTEN p.P248fs*5 c.741_742insA Homozygous
|
|
|
|
| contains Lkb1 protein 19187764-Fig-1a,6d
|
|
|
|
| contains Braf(V600E) 19274086(C)
|
|
|
|
| a stromal/osteoblastic cell line that supports osteoclast formation 10383440(C)
|
|
|
|
| African green monkey kidney cell line
|
|
|
|
| Vero CCL-81 African green monkey kidney epithelial adherent
|
|
|
|
| comment: negative for Ifna2 and Ifnb1 by Southern blot 3134658(D)
|
|
|
|
| "Primary human fibroblasts" 12110590(C)
|
|
|
|
| primary fibroblasts from a normal patient 15788536(C)
|
|
|
|
| a LPA1/LPA3 receptor antagonist 16199135(C)
|
|
|
|
| WEHI-231 CRL-1702 mouse lymphoblast suspension B lymphocyte; lymphoma
|
|
|
|
| B-cell lymphoma cell line 9535930(C)
|
|
|
|
| WI 38 CCL-75 human fibroblast adherent lung
|
|
|
|
| WiDr CCL-218 human epithelial adherent colon; colorectal adenocarcinoma
|
|
|
|
| human colon carcinoma 8609418(C)
|
|
|
|
| WIL2-NS CRL-8155 human lymphoblast suspension hybridoma fusion partner
|
|
|
|
| a B-cell line that upregulates Cd23 in response to IL4 9110977(C)
|
|
|
|
| Burkitt’s lymphoma cell line 14563837(C)
|
|
|
|
| contains a Myc T58 mutation 14563837(C)
|
|
|
|
| 34. Niklinski, J., Claassen, G., Meyers, C., Gregory, M. A., Allegra, C. J., Kaye,
F. J., Hann, S. R., and Zajac-Kaye, M. (2000) Mol. Cell. Biol. 20, 5276–5284
|
|
|
|
| WM35 CRL-2807 human spindle-shaped adherent skin, melanocyte; primary superficial spreading melanoma in radial growth phase/vertical growth phase
|
|
|
|
| contains Lkb1 protein 19187764-Fig-6d
|
|
|
|
| contains Lkb1 protein 19187764-Fig-6d
|
|
|
|
| contains Lkb1 protein 19187764-Fig-6d
|
|
|
|
| human Renal Cell Carcinoma cell line 7721786(C)
|
|
|
|
| Obiri N etal (1993) J Clin Invet 91, 88-93
|
|
|
|
| nomal human skin fibroblasts 15029243(C)
|
|
|
|
| xeroderma pigmentosum complementation group C (XPC) cells 14695167(C)
|
|
|
|
| an XPC human fibroblast cell line immortalized with SV40 and EBNA 14695167(C)
|
|
|
|
| Ku80 deficient hamster cells 15758953(C)
|
|
|
|
| a fully differentiated Tc1 line. a Cd8-pos memory T cell clone specific for malaria epitope SYVPSAEQI. These cells produce IFNg but no IL-4. 17200144(C)
|
|
|
|
| a NK-like cell line 10373548(C)
|
|
|
|
| used without explanation in 10825200(C)
|
|
|
|
| a NK-like cell line 2047859(C)
|
|
|
|
| J. Yodoi et al., J. Immunol.134, 1623 (1985).
|
|
|
|
| expresses Lck 2047859-Fig-1
|
|
|
|
| human natural killer lymphocytes that constitutively synthesize and secrete IFN-g 7479881(C)
|
|
|
|
| 38. Yodoi, J., Teshigawasa, K., Nikaido, T., Fukui, K., Noma, T.Honjo, T., Takigawa, M., Sasaki, M., Minato, N., Tsudo, M.Uchiyama,T.& Maeda,M. (1985)J.Immunol. 134,1623-1630.
|
|
|
|
| expresses mRNA for Gp130 2261637-Fig-4
|
|
|
|
| expresses mRNA for IL6R 2261637-Fig-4 3136546-Fig-3
|
|
|
|
| contain IL6 binding sites 3136546-Fig-3
|
|
|
|
| a subclone of YT 1940342(C)
|
|
|
|
| IL2 independent 1940342(C)
|
|
|
|
| expresses IL2Rb not IL2Ra 1940342(C)
|
|
|
|
| breast cancer 18037881(C)
|
|
|
|
| ZR-75-1 CRL-1500 human ascites (metastasis); ductal carcinoma epithelial adherent (mammary gland epithelium primary)
|
|
|
|
| used in 7816602 without explanation
|
|
|
|
| http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=86030402&collection=ecacc_gc
|
|
|
|
| Human brain astrocytoma HPA General Cell Collection
|
|
|
|
| 1321N1 is a human astrocytoma cell line isolated in 1972 as a sub clone of the cell line 1181N1 which in turn was isolated from the parent line U-118 MG (one of a number of cell lines derived from malignant gliomas by J Ponten (Pontén, J., Macintyre, E. H. (1968) PMID: 4313504).1321N1 has been shown to have very similar STR profile data to U-118 MG.
|
|
|
|
| in Cosmic database only as U118MG
|
|
|
|
| a clone derived from HT1080 cells stably transfected with pDW9-27CD2 and pTKNeo 7901766(M)
|
|
|
|
| parent to mutant cell lines: GAMMA1A and GAMMA2A
|
|
|
|
| A mouse T-cell clone initially established as an IL12-responsive clone (18) and later found to also respond to IL18 (11). Maintained in IL2. 11801649(C)
|
|
|
|
| 11. Ahn, H. J., S. Maruo, M. Tomura, J. Mu, T. Hamaoka, K. Nakanishi, S. Clark, M. Kurimoto, H. Okamura, and H. Fujiwara. 1997. A mechanism underlying synergy between IL-12 and IFNg-inducing factor in enhanced production of IFN-g. J. Immunol. 159:2125.
|
|
|
|
| 18. Maruo, S., H. J. Ahn, W. G. Yu, M. Tomura, M. Wysocka, N. Yamamoto, M. Kobayashi, T. Hamaoka, G. Trinchieri, and H. Fuijiwara. 1997. Establishment of an IL-12-responsive T cell clone: its characterization and utilization in the quantitation of IL-12 activity. J. Leukocyte Biol. 61:346.
|
|
|
|
| used without explanation in 8524316 (C)
|
|
|
|
| a human cell line used without more info in 10848577
|
|
|
|
| a human fibrosarcoma 8798467(C)
|
|
|
|
| parent to mutant cell lines: U1A,U2A,U3A,U4A,U5A,U6A 8798467(22,27-30)
|
|
|
|
| parent to mutant cell lines: U1A,U2A,U3A,U4A,U5A,U6A 8798467(29, 35, 42, 44, 45, 48)
|
|
|
|
| 29. John, J., R. McKendry, S. Pellegrini, D. Flavell, I. M. Kerr, and G. R. Stark.
1991. Isolation and characterization of a new mutant human cell line unre-
sponsive to alpha and beta interferons. Mol. Cell. Biol. 11:4189–4195.
|
|
|
|
| 35. Leung, S., S. A. Qureshi, I. M. Kerr, J. E. Darnell, Jr., and G. R. Stark. 1995.
Role of STAT2 in the alpha interferon signaling pathway. Mol. Cell. Biol. 15:
1312–1317.
|
|
|
|
| 42. McKendry, R., J. John, D. Flavell, M. Mu¨ller, I. M. Kerr, and G. R. Stark.
1991. High-frequency mutagenesis of human cells and characterization of a
mutant unresponsive to both alpha- and beta-interferons. Proc. Natl. Acad. Sci. USA
88:11455–11459.
|
|
|
|
| 44. Muller, M., J. Briscoe, C. Laxton, D. Guschin, A. Ziemiecki, O. Silvennoinen, A. G. Harpur, G. Barbieri, B. A. Witthuhn, C. Schindler, S. Pellegrini, A. F. Wilks, J. N. Ihle, G. R. Stark, and I. M. Kerr. 1993. The protein tyrosine kinase JAK1 complements defects in interferon-alpha/beta and -gamma signal transduction. Nature (London) 366:129–135. 8232552
|
|
|
|
| 48. Pellegrini, S., J. John, M. Shearer, I. M. Kerr, and G. R. Stark. 1989. Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway. Mol. Cell. Biol. 9:4605–4612. 2513475
|
|
|
|
| human fibrosarcoma derived from HT1080 7537214(C)
|
|
|
|
| The Th1 clone 3F6 was derived by OVA stimulation from DO11.10 transgenic splenocytes in the presence of anti-IL-4 followed by limiting dilution cloning (33, 34), and it was maintained by 3-d stimulation of 107 T cells with 3 x 10s Balb/c splenocytes, 500 nM OVA, and 40 U/ml IL-2 in supplemented IMDM with 10% FCS, followedby a 7-d period of expansion in media with 1L2 alone.
|
|
|
|
| 33. Murphy, K.M., A.B. Heimberger, and D.Y. Loh. 1990. Induction by antigen of intrathymic apoptosis of CD4-pos CD8-pos TCR-lo thymocytes in vivo. Science(Wash. DC). 250:1720-1723.
34. Hsieh, C.-S., A.B. Heimberger, J.S. Gold, A. O'Garra, and K.M. Murphy. 1992. Differential regulation of T helper phenotype development by interleukins 4 and 10 in an alpha-beta-T-cell-receptor transgenic system. Proc Natl. Acad. Sci. USA. 89:6065-6069.
|
|
|
|
| 3T3 subclone transformed with a temperature-sensitive mutant v-Abl (24). The v-Abl kinase in D5 cells is inactive at 39°C, but kinase activity is restored upon shifting cells to 32°C. 11003655(C)
|
|
|
|
| 24. Renshaw, M. W., E. T. Kipreos, M. R. Albrecht, and J. Y. Wang. 1992. Oncogenic v-Abl tyrosine kinase can inhibit or stimulate growth, depending on the cell context. EMBO J. 11:3941–3951.
|
|
|
|
| amplified for Mdm2 9529248(C)
|
|
|
|
| 3T3DM cells express several alternatively processed species of Mdm2 protein (Figure 2D, lane 20–21, p90/p85, p76/p74, and in very low amounts, p57) (Olson et al., 1993; Barak et al., 1994). 9529248(C)
|
|
|
|
| Olson, D.C., Marechal, V., Momand, J., Chen, J., Romocki, C., and Levine, A.J. (1993). Identification and characterization of multiple mdm-2 proteins and mdm-2-p53 protein complexes. Oncogene 8, 2353–2360.
|
|
|
|
| Barak, Y., Gottlieb, E., Juven-Gershon, T., and Oren, M. (1994). Regu- lation of mdm2 expression by p53: alternative promoters produce transcripts with nonidentical translation potential. Genes Dev. 8, 1739–1749.
|
|
|
|
| express endogenous EgfR 9363897(C)
|
|
|
|
| 11. Argetsinger, L. S. et al. Growth hormone, interferon-g, and leukemia inhibitory factor promoted tyrosyl phosphorylation of insulin receptor substrate-1. J. Biol. Chem. 270, 14685–14692 (1995).
|
|
|
|
| differentiation: 2173694(M)
|
|
|
|
| to adipocytes was induced by incubating the cells for 2 days in the
same volumes of high glucose DMEM containing 10% fetal bovine
serum, 5 fig/ml insulin, 0.25 pM dexamethasone, 0.5 mM 3-isobutyl-
l-methylxanthine, 50 units/ml penicillin, and 50 fig/ml streptomycin
sulfate. The cells were then incubated for an additional 2 days in the
same media without 0.25 ELM dexamethasone and 0.5 mM 3-isobutyl-
1-methylxanthine. The cells were then maintained for an additional
6 to 10 days in high glucose DMEM containing 10% fetal bovine
serum prior to measuring sugar uptake or harvesting cell membranes.
At this time, greater than 95% of the cells appeared to express the
|
|
|
|
| Rat fibroblast 12049732(C) 9038154(C)(calls it 3YI)
|
|
|
|
| used without explanation in 16129686
|
|
|
|
| 0734, Japan Cell Resource Bank (JCRB) rat fibroblast 10318861(M)
|
|
|
|
| from 11907583: 4E3 cells were generated by stable transfection of Jurkat cells with human TNF-RII cDNA16, provided by M. Lenardo. [16]
|
|
|
|
| 16. Chan, F. K. & Lenardo, M. J. A crucial role for p80 TNF-R2 in amplifying p60 TNF-R1 apoptosis signals in T lymphocytes. Eur. J. Immunol. 30, 652 – 660 (2000).
|
|
|
|
| 9HTEo- human airway epithelial cells (tracheal; a gift from Dr. Dieter Gruenert, University of California, San Francisco) (23) 12122011(C)
|
|
|
|
| 23. Gruenert, D. C., Basbaum, C. B., Welsh, M. J., Li, M., Finkbeiner, W. E., and
Nadel, J. A. (1988) Proc. Natl. Acad. Sci. U. S. A. 85, 5951–5955
|
|
|
|
| an A-T cell line homozygous for the truncation mutation R3047X 9733514(C)
|
|
|
|
| 1.3E2, a Nemo-deficient murine pre-B cell line 19675659(C)
|
|
|
|
| derived from 70Z3 cells 18635759(C)
|
|
|
|
| G. Courtois, S. T. Whiteside, C. H. Sibley, A. Israel, Mol Cell Biol 17, 1441 (1997).
|
|
|
|
| human mammary epithelial cell strain immortalized with human appilloma virus E6
|
|
|
|
| 22. Band, V., De Caprio, J. A., Delmolino, L., Kulesa, V., and Sager, R. (1991)
J. Virol. 65, 6671– 6676
|
|
|
|
| 16HBE14o- human airway epithelial cells (bronchial; a gift from Dr. Gruenert; 24) 12122011
|
|
|
|
| 24. Cozens, A. L., Yezzi, M. J., Kunzelmann, K., Ohrui, T., Chin, L., Eng, K., Finkbeiner, W. E., Widdicombe, J. H., and Gruenert, D. C. (1994) Am. J.
Respir. Cell Mol. Biol. 10, 38–47
|
|
|
|
| 32D cells lack IL2Rb 8700888(C)
|
|
|
|
| 32. Otani,H.,Siegel,J.P.,Erdos,M.,Gnarra,J.R.,Toledano,M. B.,
Sharon, M., Mostowski, H., Feinberg, M. B., Pierce, J.H. &Leonard, W. J.(1992)Proc. Natl.Acad. Sci. USA 89,2789-2793.
|
|
|
|
| 33. Otani,H.,Erdos,M.&Leonard,W.J.(1993)J.Biol.Chem.268,22733-22736.
|
|
|
|
| IL3 dependent mouse lymphoblast from bone marrow
|
|
|
|
| 32D Clone 3 CRL-11346† mouse lymphoblast mixed bone marrow
|
|
|
|
| 12417588(C) 32D cells are murine myeloid progenitors that express few endogenous insulin receptors and no Irs proteins and require interleukin-3 for growth (48)
|
|
|
|
| 48. Myers, M. G., Jr., Zhang, Y., Aldaz, G. A. I., Grammer, T. C., Glasheen, E. M., Yenush, L., Wang, L. M., Sun, X. J., Blenis, J., Pierce, J. H., and White, M. F. (1996) Mol. Cell. Biol. 16, 4147– 4155
|
|
|
|
| IL-3-dependent myeloid cells 10654938(C)
|
|
|
|
| might be: ATCC CRL-11346 32D Clone 3 mouse lymphoblast from bone marrow
|
|
|
|
| an A-T cell line homozygous for the missense mutation E2904G 9733514(C)
|
|
|
|
| 11460167(C) used without explanation
|
|
|
|
| 12566447(C) mouse pre-B cells
|
|
|
|
| 70Z/3 TIB-158 mouse lymphoblast suspension
pre-B lymphoblast; methylnitrosourea-induced lymphoma
|
|
|
|
| parental cells of 13E2 Nemo-null cells 18635759(C)
|
|
|
|
| mammoplasty-derivied normal human mammary epithelial cell
|
|
|
|
| 21. Band, V., and Sager, R. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 1249-1253
|
|
|
|
| express normal levels of Pin1 11432833-Fig-2d
|
|
|
|
| used without explanation in 15525938(C)
|
|
|
|
| p53 is already stabilized by adenoviral proteins expressed endogenously in 293F cells (18).
|
|
|
|
| 18. Grand, R. J. et al. The high levels of p53 present in adenovirus early region 1-transformed human cells do not cause up-regulation of MDM2 expression. Virology 210, 323–334 (1995).
|
|
|
|
| 786-O CRL-1932 human epithelial adherent kidney; renal cell adenocarcinoma
|
|
|
|
| CDKN2A p.? c.1_150del150 Homozygous
PTEN p.Q149* c.445C>T Homozygous
TP53 p.P278A c.832C>G Heterozygous
TP53 p.? c.560-2A>G Heterozygous
VHL p.G104fs*55 c.311delG Homozygous
|
|
|
|
| mouse melanoma cell line isolated from neoplasitic lesions raised in HGF-transgenic-UV irradiated mice 19274086(C)
|
|
|
|
| 33: Leshem Y, Gitelman I, Ponzetto C, Halevy O. Preferential binding of Grb2 or
phosphatidylinositol 3-kinase to the met receptor has opposite effects on
HGF-induced myoblast proliferation. Exp Cell Res. 2002 Apr 1;274(2):288-98.
PubMed PMID: 11900489.
|
|
|
|
| contains WT Braf 19274086(C)
|
|
|
|
| used without explaination in 19274086
|
|
|
|
| 33: Leshem Y, Gitelman I, Ponzetto C, Halevy O. Preferential binding of Grb2 or
phosphatidylinositol 3-kinase to the met receptor has opposite effects on
HGF-induced myoblast proliferation. Exp Cell Res. 2002 Apr 1;274(2):288-98.
PubMed PMID: 11900489.
|
|
|
|
| contains WT Braf 19274086(C)
|
|
|
|
| CCD-1070Sk ATCC CRL-2091 human skin fibroblast
|
|
|
|
|
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