|
|
 | hADCs: human (Mature) Activated Dendritic Cells
|
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|
|
 | hIDCs are cultured for 36 hr with c-irradiated L-cells expressing Tnfsf5 (Cd40L).
|
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|
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| hAECs: human Aortic Endothelial Cells
|
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| hAMCs: human Alveolar Macrophages
|
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|
|
| Cells are collected by bronochoalveolare lavage 17658277(M)
|
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|
| hASMCs: human Aortic Smooth Muscle Cells
|
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|
| hCAECs: human Coronary Artery Endothelial Cells
|
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|
|
| from Clonetics 11029289(M)
|
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|
|
| hBECs: human Bronchial Epithelial Cells
|
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|
| obtained from Clonetics (now Lonza)
|
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|
|
| hESKs: human ESophageal Keratinocytes
|
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| hFibros: human Fibroblasts
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| hIDC1: human Immature Dendritic Cells
|
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| adherent hPBMs are cultured for 6 days in BMS + Csf2 + IL4
|
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| hIDC2: human Immature Dendritic Cells
|
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|
|
| Cd14+ hPBMs are cultured for 6-8 days in BMS + Csf2 + IL4
|
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|
|
| non-adherent cells are used
|
|
|
|
| used in: 15939879 16895974
|
|
|
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| hIECs: human Intestinal Epithelial Cells
|
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| hMDM1: human Monocyte Derived Macrophages (elutriated)
|
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| Elutriated Monocyte Suspensions from: University of Nebraska Medical Center
|
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| "By balancing centrifugal force against the opposing buffer flow, lymphocytes, which are about 6-8 mm and monocytes, which are 8-10 µm, will be selectively removed from the mixture."
|
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| Elutriated Monocyte Suspensions from Advanced Biotechnologies Inc
|
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| "Elutriated monocyte suspensions are >90% pure when analyzed by a variety of techniques. ABI documents purity during the elutriation process by multisizer analysis of purified monocytes, using statistical data to establish that >90% of the cell population has a mean cell size that is greater than small monocytes (approximately 8.5 microns). Final purity is confirmed by CD14 cell surface antigen distribution using immunofluorescence (IFA), with CD15 antigen tested by IFA as a control marker for granulocytes, which typically account for the majority of contaminating white blood cells in monocyte fractions."
|
|
|
|
| used by: 15953028, 15953029, 16113308, 16996713, 16816147
|
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|
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| hMDM2: human Monocyte Derived Macrophages (incubated on Teflon)
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| same as human PBMs but incubated on Teflon dishes for 5-7 days before enriching for macrophages by adherence to plastic
|
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|
| no clues as to what the 5-7 days on Teflon are suppose to do.
|
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| Peripheral blood mononuclear cells were plated at a density of 5 106 cells/ml in Teflon six-well plate inserts in RPMI (supplemented with 10% fetal bovine serum, 2 mM L-glutamine, 1% [vol/vol] nonessential amino acids, 10 mM HEPES) and incubated at 37°C for 3 days. Cell monolayers were then scraped and washed in cold Hanks’ balanced salt solution before plating in a 24-well plate at a density of 5 x 106 cells/well. Monolayers were then incubated for 2 days in RPMI with 10% fetal bovine serum before nonadherent cells were removed by washing with cold Hanks’ balanced salt solution. The resulting MDMs were allowed to rest by incubating an additional 1 to 2 days in RPMI lacking serum before use. Viability was assessed by trypan blue dye exclusion and monocyte enrichment was established with the alpha-naphthyl acetate (nonspecific esterase) (Sigma). 4977957(M)
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| used in: 15953028 15953029 16926403
|
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| hMDM3: hPBMs + CD14 selection
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| using Miltenyi Biotec Isolation Kit I
|
|
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| human PBMs depleted of CD3, CD7, CD19, CD45RA, CD56, and IgE expressing cells
|
|
|
|
| using Miltenyi Biotec Isolation Kit II
|
|
|
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| human PBMs depleted of CD3, CD7, CD19, CD56, CD123,and CD235a expressing cells
|
|
|
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| using Miltenyi Cd14 Microbeads
|
|
|
|
| direct selection for Cd14 positive cells from human PBMs
|
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|
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| used in: 16373510 16895974 17372002
|
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|
|
| hMDM4: hPBMs + CD14 selection + Csf1 differentiation
|
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| Cd14+ hPBMs are cultured for 6-7 days in BMS + Csf1.
|
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|
|
| used in: 17163980 18496526
|
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|
|
| hMDM5: hPBMs + Csf1 differentiation
|
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|
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| hPBMs cultured for 2-3 days in Csf1
|
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|
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| express TLR4 [FACS] 14630816(D)
|
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|
|
| hMDM6: adherent hPBMs cultured on plastic for 7 days
|
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|
| hPBMs: human Peripheral Blood Mononuclear cells
|
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| isolated from fresh blood on Ficoll gradients
|
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|
|
| used by: 16574669 15925273 16816147
|
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|
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| hPBMonos: human Peripheral Blood Monocytes
|
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|
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| hPBMs further purified on 46% iso-osmotic Percoll to remove lymphocytes
|
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|
| hPBTC1s: human Peripheral Blood T-cells
|
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|
|
| hPBMs were depleted of adherent cells
|
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|
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| Non-adherent cells were passed over a nylon column in BMS
|
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|
|
| hPBTC2s: human Peripheral Blood T-cells
|
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| hPBMs sorted to remove monocytes, B, NK, gamma T-cells, delta-T-cells
|
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|
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| methods not given 10790433(M)
|
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|
|
| hPDCs: human Plasmacytoid Dendritic Cells
|
|
|
|
| human Cd304(BDCA4/Nrp1)-pos cells are cutured with IL3 for 7 days
|
|
|
|
| hPBMs are enriched with anti-Cd123(IL3Rax) or anti-BDCA4(Nrp1) Abs
|
|
|
|
| hPBMs are enriched with anti-Cd304(BDCA4/Nrp1) Ab
|
|
|
|
| MHCII-pos Cd123-pos > 95%
|
|
|
|
| see intro 15345224 on IPC (Interferon producing cells)
|
|
|
|
| hSNFs: human Synovial Fibroblasts
|
|
|
|
| do not express TLR4 [FACS] 14630816(D)
|
|
|
|
| May be the same as primary human Synoviocytes used in 16371247 without explanation
|
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|
|
| hSVKs: human SV40 transformed keratinocytes
|
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|
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| hTBCs: human tonsillar B cells
|
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|
|
| used in 9020128, described elsewhere:
|
|
|
|
| 11. Manie ́ , S., Astier, A., Wang, D., Phifer, J., Chen, J., Lazarovits, A., Morimoto,
C., and Freedman, A. (1996) Blood 87, 1855–1861
|
|
|
|
| hUVEC: human umbilical cord endothelial cells
|
|
|
|
| do not express TLR4 [FACS] 14630816(D)
|
|
|
|
| mADCs: mouse Alveolar Dendritic Cells
|
|
|
|
| Cells are collected by bronchoalveolar lavage
|
|
|
|
| Cells are selected to be Cd11b+ Ly75+ Cd11b-
|
|
|
|
| Resulting cell populations are: >95% CD11c+/DEC-205+/CD11b-/GR-1- 16272336(M)
|
|
|
|
| mAMCs: mouse Alveolar Macrophages
|
|
|
|
| Cells are collected by bronchoalveolar lavage
|
|
|
|
| mAstrocytes: mouse Astrocytes
|
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|
|
| mBMcDCs: mouse Bone Marrow derived Conventional Dendritic Cells
|
|
|
|
| mBMfDCs sorted for: Cd11c-high B220-low Cd11b-high
|
|
|
|
| mBMfDCs sorted for: Cd11c-pos B220-neg
|
|
|
|
| express [RT-PCR]: TLR4, TLR9, Myd88, Irak1, Irak2, Irak4, Traf6 15153500(D)
|
|
|
|
| mBMCs: mouse Bone Marrow Cells
|
|
|
|
| Total population from femurs and tibiae of mice, red blood cells lysed 15034168(M)
|
|
|
|
| Cd11c-pos B220-pos 1-2% 16612387-Fig-S1a
|
|
|
|
| Cd11c-neg B220-pos ~50% 16612387-Fig-S1a
|
|
|
|
| Cd11c-neg B220-neg ~50% 16612387-Fig-S1a
|
|
|
|
| B220-pos IgM-neg 12.4% 15665823-Fig-S1c
|
|
|
|
| B220-pos IgM-pos 9.2% 15665823-Fig-S1c
|
|
|
|
| mBMDCs: mouse Bone Marrow derived Dendritic Cells
|
|
|
|
| Incubate for 5-10 days in Csf2 (GM-CSF) containing medium
|
|
|
|
| sometimes conditioned medium from B78-Hi cells is used as a source of Csf2
|
|
|
|
| The non adherent cells are used
|
|
|
|
| 1161442(M) also used in: 15557183
|
|
|
|
| Bone marrow was obtained from BALB/c mice femurs and tibias.
|
|
|
|
| RBC were lysed with 0.17 M Tris-NH4Cl buffer and lymphocytes and MHC class-II-positive cells were eliminated by magnetic bead separation using a cocktail of antibodies.
|
|
|
|
| BioMag goat anti-rat IgG-coated beads (Poly-sciences, Inc., Warrington, PA) were used to eliminate cells labeled with anti-CD4 Gk1.5 hybridoma (ATCC) supernatant, anti-CD8 2.43 hybridoma (ATCC) supernatant, anti-I-Ad/I-Ed (2G9: PharMingen, San Diego, CA), and anti-CD45R/B220 (RA3-6B2: PharMingen) following the protocol suggested by the manufacturer.
|
|
|
|
| The purified precursors were plated in RPMI medium containing 5% FCS (or 1% syngenic NMS), gentamicin (50 g/ml), 500 U/ml rGM-CSF (PreproTech EC Ltd.), and 20 ng/ml IL-4 (Boehringer Mannheim).
|
|
|
|
| One day prior to use the cells were replated to eliminate contaminating macrophages adherent to the plate.
|
|
|
|
| >80% Cd11c+ 16895974(M) 16622218(M)
|
|
|
|
| 70-80% Cd11c-pos Cd11b-pos 16039576(D)
|
|
|
|
| mBMfDCs: mouse Bone Marrow derived Dendritic Cells
|
|
|
|
| Incubate for 6-11 days in Flt3lg containing medium
|
|
|
|
| The non adherent cells are used
|
|
|
|
| used in 15800576, 14976261, 16306936
|
|
|
|
| 40-50% "pDCs" 16210631(C)
50-60% "myeloid" DCs 16210631(C)
|
|
|
|
| 14% 'pDCs' Cd11c-pos B220-pos 16239576-Fig-S1a
35-38% 'cDCs' Cd11c-pos B220-neg 16239576-Fig-S1b
|
|
|
|
| 9-10% 'pDCs' Cd11c-pos B220-pos 16039576-Fig-6c
25-27% 'cDCs' Cd11c-pos B220-neg 16039576-Fig-6c
|
|
|
|
| 17-22% 'pDCs' Cd11c-pos B220-pos 16039576-Fig-6d
47-45% 'cDCs' Cd11c-pos B220-neg 16039576-Fig-6d
|
|
|
|
| mBMMs: mouse Bone Marrow derived Macrophages
|
|
|
|
| Cells are flushed from mouse bones
|
|
|
|
| Incubated for 5-8 days in Csf1 containing medium
|
|
|
|
| sometimes conditioned medium from L929 cells is used as a source of Csf1
|
|
|
|
| The adherent cells are used
|
|
|
|
| >99% Mac1-pos 16848641(M) 12686550(M)
|
|
|
|
| 94-99% Cd11b-pos 12354379(M)
|
|
|
|
| mBMNs: mouse Bone Marrow derived Neutrophils
|
|
|
|
| Murine bone marrow neutrophils were prepared by centrifugation
through Percoll gradients. Purified neutrophils were suspended in
Hanks’ buffer (0.14 M NaCl, 5.4 mM KCl, 1 mM Tris, 1.1 mM CaCl2,
0.4 mM MgSO4 , and 1 mM HEPES [pH 7.2]) containing 5 mg/ml
BSA.
|
|
|
|
| mBMpDCs: mouse Bone Marrow derived Plasmacytoid-like Dendritic Cells
|
|
|
|
| mBMfDCs sorted for: Cd11c-high B220-high Cd11b-low 14634101(M)
|
|
|
|
| mBMfDCs sorted for: Cd11c-high Cd11b-low 15345224(M)
|
|
|
|
| mBMfDCs sorted for: Cd11c-pos B220-pos 16612387(M)
|
|
|
|
| mBMfDCs sorted for: B220-pos 15153500(M)
|
|
|
|
| express [RT-PCR]: (TLR4), TLR9, Myd88, Irak1, Irak2, Irak4, Traf6 15153500(D)
|
|
|
|
| mBMfDCs sorted with: MoAb-120G8 and anti-B220(Cd45)
|
|
|
|
| Antibody-120G8, stains a small subset of CD11c-low spleen cells with high specificity. This population produces high amounts of Ifna upon in vitro viral stimulation. Both ex vivo- and in vitro-derived 120G8-pos cells display a phenotype identical with that of mouse pDCs: B220-high Ly6C-high Gr1-low CD11b-neg CD11c-low. MAb 120G8 stains all and only B220-high Ly6C-high CD11c-low pDC in all lymphoid organs. [Immunex catalog]
|
|
|
|
| comment: IRF-8 transduction (into Irf8-null BM progenitors)led to the generation of both pDCs and non-pDCs, while control vector transduction produced only non-pDCs.
|
|
|
|
| 32. Tsujimura, H., T. Tamura, and K. Ozato. 2003. Cutting edge: IFN consensus
sequence binding protein/IFN regulatory factor 8 drives the development of type
I IFN-producing plasmacytoid dendritic cells. J. Immunol. 170:1131.
|
|
|
|
| mBMPOs: mouse Bone Marrow derived PreOsteoclasts
|
|
|
|
| Primary BMMs were prepared as described previously (Faccio et al., 2003b) with slight modification. Marrow was extracted from femora and tibiae of 6- to 8-week-old mice with a-MEM and cultured in a-MEM containing 10% inactivated fetal bovine serum, 100 IU/ml penicillin, and 100 mg/ml streptomycin (a-10 medium) with 1:10 CMG condition media (Takeshita et al., 2000) in bacterial plastic. Cells were incubated at 37oC in 6% CO2 for 3 days and then washed with PBS and lifted with 13 trypsin/EDTA (Invitrogen, Carlsbad, CA) in PBS. A total of 5 x 103 cells were cultured in 200 ml a-MEM containing 10% heat-inactivated FBS with 100 ng/ml GST-RANKL and 40 ng/ml of mouse recombinant M-CSF in 96-well tissue culture plates, some containing sterile bone slices. Cells were fixed and stained for TRAP activity after 5 days in culture, using a commercial kit (Sigma 387-A, St. Louis, MO). For actin ring stain- ing, cells were fixed in 4% paraformaldehyde, permeabilized in 0.1% Triton X-100, rinsed in PBS, and immunostained with Alexa 488 phalloidin (Molecular Probes). To quantitate resorption lacunae, cells were removed from bone sli- ces with mechanical agitation. Bone slices were incubated with peroxidase- conjugated wheat germ agglutinin (Sigma) for 1 hr and stained with 3, 3'-diaminobenzidine (Sigma). For preosteoclast generation, 1.5 x 106 BMMs were plated per 10 cm tissue culture dish and cultured in 40 ng/ml M-CSF and 100 ng/ml GST-RANKL for 2-3 days. 18691974(M)
|
|
|
|
| Pre-fusion osteoclasts were prepared as described previously (26). Briefly, pre-fusion osteoclasts were obtained from co-cultures of osteoblastic MB1.8 cells and murine bone marrow cells in alpha-MEM containing 10% FBS and 10 nM 1-alpha,25-dihydroxyvitamin D3. After 5 days in co-culture, pOCs were released from dishes with enzyme-free cell dissociation buffer after removing MB1.8 cells with 0.1% (w/v) collagenase and dispase in PBS. 12514172
|
|
|
|
| 26. Wesolowski, G., Duong, L. T., Lakkakorpi, P. T., Nagy, R. M., Tezuka, K.,
Tanaka, H., Rodan, G. A., and Rodan, S. B. (1995) Exp. Cell Res. 219,
679-686
|
|
|
|
| mCECs: mouse Colon Epithelial Cells
|
|
|
|
| used in 17197697 without explanation
|
|
|
|
| mEFs: mouse Embyo Fibroblasts
|
|
|
|
| mEHNs: mouse Embryonic Hippocampal-Cortical Neurons
|
|
|
|
| used in 12194869(M) refers to
|
|
|
|
| Rameau, G.A., et al. (2000). Role of NMDA receptor functional domains in excitatory cell death. Neuropharmacology 39, 2255–2266
|
|
|
|
| mELCs: mouse Embryonic Liver cells
|
|
|
|
| mESCs: mouse Embryonic Stem cells
|
|
|
|
| mHepatocytes: mouse Hepatocytes
|
|
|
|
| Primary hepatocytes were isolated from the livers of ICR male (5- to 8-week-old) mice by a
two-step perfusion procedure using 0.025% collagenase buffered with 0.1 M HEPES (pH
7.5), as described by Klaunig et al. [1981]. They were plated at a density of 104 cells per cm2 in 12-well plates or 60-mm diameter dishes in a mixture of 75% minimal essential medium and 25% medium 199, supplemented with fetal bovine serum (10%) and insulin (10 -7 M). After cell attachment, which occurred 4 h after plating, the medium was renewed with the same medium lacking FBS and supplemented with dexamethasone (10-9 M), sodium pyruvate (10 mM), and bovine serum albumin (0.1%). It was renewed every day thereafter. 16619271(M)
|
|
|
|
| Klaunig JE, Goldblatt PJ, Hinton DE, Lipsky MM, Chacko J, Trump BF. 1981. Mouse liver cell culture. I. Hepatocyte isolation. In Vitr 17:913 – 925.
|
|
|
|
| 3-month-old mice were anesthetized with Somnitol, and the liver was perfused first with EGTA and then with collagenase (type IV; Sigma). The liver was carefully dissected out, and hepatocytes were carefully released into a Petri dish containing Williams Media E (Invitrogen) supplemented with L-Glutamine (Gibco) by gentle agitation with fine forceps. The hepatocytes were then filtered through a nitex membrane and centrifuged at 250 X g for 5 min. The cell pellet was resuspended in 30 ml of Williams Media E, carefully triturated, and centrifuged again at 250 X g for 5 min. The cell pellet was then resuspended in 20 ml of Complete Media (Williams Media E supplemented with 10% FCS, penicillin, and streptomycin), counted on a hemocytometer, and 2.5 x 105 viable cells were seeded onto 35-mm plates coated with fibronectin. Two hours later the cells were washed, and 2 ml of fresh complete media was added. The cells were used for experiments the following day. 18697935(M)
|
|
|
|
| mHMCs: mouse Hepatic Mononuclear Cells
|
|
|
|
| Liver homogenates purified on a Percoll gradient
|
|
|
|
| This is a mixed population of liver macs (Kupfer cells) and T- cells [Cur Prots Immunol]
|
|
|
|
| Fetal liver-derived macrophages generated from E14.5 embryo liver were plated and cultured in RPMI with 10% fetal bovine serum plus L cell media for 7 days as described (Ogawa et al., 2004).
|
|
|
|
| Ogawa, S., Lozach, J., Jepsen, K., Sawka-Verhelle, D., Perissi, V., Sasik, R., Rose, D.W., Johnson, R.S., Rosenfeld, M.G., and Glass, C.K. (2004). An NCoR transcriptional checkpoint controlling AP-1-dependent gene networks required for macrophage activation. Proc. Natl. Acad. Sci. USA 101, 14461–14466.
|
|
|
|
| mKidneyCells: mouse Kidney Cells
|
|
|
|
| mKupfferCells: mouse Kupffer Cells
|
|
|
|
| Kupffer cells were isolated from mice with various genetic backgrounds according to a method
previously described (Seki et al. 2001).
|
|
|
|
| Seki, E., Tsutsui, H., Nakano, H., et al. (2001) LPS-induced IL-18 secretion from murine Kupffer cells independently of MyD88 that is critically involved in induction of production
of IL-12 and IL-1β. J. Immunol. 166, 2651–2657.
|
|
|
|
| mLECs: mouse Lung Epithelial Cells
|
|
|
|
| MLEC were isolated by modifications to established protocols using rat anti-mouse CD31 (i.e. PECAM) monoclonal antibody-coated magnetic beads and characterized by panels of endothelial cell-specific markers (Tang et al., 2002). MLEC were grown in low-glucose DMEM, 20% FBS, 20 mM HEPES, 2 mM glutamine, 100 U/mL penicillin, 100 Ag/mL streptomycin and 200 Ag mL Endo Gro (VEC Technologies, Inc) at 37 -C in an atmosphere with 5% CO2.
|
|
|
|
| Tang, Z.L., Wasserloos, K.J., Liu, X.H., Reynolds, I.J., Pitt, B.R., St. Croix C.M., 2002. Nitric oxide decreases the sensitivity of pulmonary endothelia cells to LPS-induced apoptosis in a zinc-dependent fashion. Mol. Cell Biochem. 234/235, 211 – 217.
|
|
|
|
| mLFs: mouse Lung Fibroblasts
|
|
|
|
| used in 14556004(M) 12855817(NMR)
|
|
|
|
| mLNBCs: mouse Lymph Node derived B Cells
|
|
|
|
| mLNCs: mouse Lymph Node cells
|
|
|
|
| Cd4-pos Cd8-neg 44.9% 15665823-Fig-S1c
|
|
|
|
| Cd4-neg Cd8-pos 24.2% 15665823-Fig-S1c
|
|
|
|
| Cd4-pos Cd8-pos 0.4% 15665823-Fig-S1c
|
|
|
|
| mMECs: mouse Mammary Epithelial Cells
|
|
|
|
| mOLCs: mouse Osteoclast-Like Cells
|
|
|
|
| Prefusion osteoclast-like cells (pOCs) and multinucleated osteoclast-like cells (OCLs) were prepared as previously described (Duong et al., 1998). Briefly, bone marrow cells isolated from 6-week-old mice were co-cultured with osteoblastic MB1.7 cells for 5 days in the presence of 10 nM 1-alpha,25[OH]2D3. Similar to MB1.8 cells, MB1.7 cell line was derived from mouse calvarial bone marrow and selected by their potential in supporting osteoclastogenesis, in the presence of 10 nM vitamin D3 (Wesolowski et al., 1995). Prefusion osteoclast-like cells (with purity of 85–95%) were released from dishes with 10 mM EDTA, after removing MB1.7 cells with collagenase-dispase. Alternatively, the co-cultures were kept for 6 days to achieve OCLs and purified as previously described (Duong et al., 1998). OCLs (95% purity) were left to recover for 1 h in ing 10% fetal calf serum prior to preparation of OCL lysate. 16600665(M)
|
|
|
|
| mPBCs: mouse Peripheral Blood Cells
|
|
|
|
| Lymphocytes 69.1% 15665823-Fig-S1c
|
|
|
|
| Polymorphonuclear cells 17.9% 15665823-Fig-S1c
|
|
|
|
| NK (Dx5-pos IL2Rb-pos) 4.0% 15665823-Fig-S1c
|
|
|
|
| NK(IL2Rb-pos TCRb-neg) 4.3% 15665823-Fig-S1c
|
|
|
|
| mPECs: mouse Elicited Peritoneal Macrophages
|
|
|
|
| Mice are injected intraperitoneally with 4% thioglycollate or 10% proteose peptone
|
|
|
|
| Macrophages are collected by peritoneal lavage 3-4 days later
|
|
|
|
| Cells incubated for 2 hrs.
|
|
|
|
| mPodocytes: mouse Podocytes
|
|
|
|
| Immortalized mouse podocytes (a gift from P. Mundel) were cultured in RPMI 1640 supplemented with 10% FCS and 10 ng/ml interferon-gamma (Life Technologies, Inc.) at 33 °C.
|
|
|
|
| mRPECs: mouse Resident Peritoneal Macrophages
|
|
|
|
| Macrophages are collected by peritoneal lavage
|
|
|
|
| Cells incubated for 2 hrs.
|
|
|
|
| mSACs: mouse Spleenic Adherent Cells
|
|
|
|
| cells from spleens were allowed to adhere in RPMI/FBS
|
|
|
|
| mSBCs: mouse Spleen derived B-Cells
|
|
|
|
| mSCs negatively selected for: Cd43 Cd4 Ter-119
|
|
|
|
| purifed from spleens by negative selection with Cd43 mAb 12055629(M)
|
|
|
|
| purified from spleens by positive selection with Cd45R Ab 12855817(M)
|
|
|
|
| purified from spleens by positive selection with Cd19 Ab 15345224(M)
|
|
|
|
| positive for TLR9-mRNA [RT-PCR]
|
|
|
|
| purified from spleens by negative selection with anti-Thy1-Ab + complement
|
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|
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| mScDCs: mouse Spleen derived Conventional Dendritic Cells
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| mSCs sorted for: Cd11c-pos B220-neg
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|
| pos for TLRs 1,2,3,4,5,6,7,8,9 mRNA [RT-PCR] 12672047(D)
|
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|
|
| pos for TLRs 1,2,3,4,(5),6,8,9 mRNA [RT-PCR] 12672047(D)
|
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|
|
| neg for TLR7 mRNA [RT-PCR] 12672047(D) 15711573(D)
|
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| pos for TLR3 mRNA[RT-PCR] 15711573(D)
|
|
|
|
| pos for TLRs 1,2,3,4,5,6,7,8,9 mRNA [RT-PCR] 12672047(D)
|
|
|
|
| mSCs sorted for: Cd11c-high Ly6c-neg B220-neg
|
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| 15800576(M) variation 15492225(M)
|
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|
|
| mSCs negatively selected for: Cd5 and Cd19 (removes T and B cells)
|
|
|
|
| sorted for: Cd11c-pos B220-neg
|
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|
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| mSCs enriched for Cd11c-pos cells
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|
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| sorted for: CD11c-pos B220-neg CD3e-neg CD19-neg NK1.1-neg
|
|
|
|
| spleens are digested with collagenase-A or liberase-CI, EDTA or DNaseI
|
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|
|
| Cd4-pos Cd8-neg 15.2% 15665823-Fig-S1c
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|
| Cd4-neg Cd8-pos 11.9% 15665823-Fig-S1c
|
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|
|
| B220-pos Cd3-neg 47.7% 15665823-Fig-S1c
|
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|
|
| B220-neg Cd3-pos 43.9% 15665823-Fig-S1c
|
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|
|
| B220-pos Cd11b-neg 45.9% 15665823-Fig-S1c
|
|
|
|
| B220-pos Cd11b-pos 11.0% 15665823-Fig-S1c
|
|
|
|
| 1-2% mPDCA1-pos Cd11c-int (mSpDCs) 16030576-Fig-S1c
|
|
|
|
| 1-2% mPDCA1-neg Cd11c-pos (mScDCs) 16030576-Fig-S1c
|
|
|
|
| mSDCs: mouse Spleen derived Dendritic Cells
|
|
|
|
| mSCs sorted for: Cd11c-pos
|
|
|
|
| approx 20% pDCs: Cd11c-pos B220-pos 16612387-Fig-S1c
|
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|
|
| approx 60% cDCs: Cd11c-pos B220-neg 16612387-Fig-S1c
|
|
|
|
| mSECs: mouse Skin Epithelial Cells
|
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|
|
| mSFs: mouse Skin Fibroblasts
|
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|
|
| To prepare skin fibroblasts (SFs), mouse body skin was shaved, cut into small pieces, and then subjected to trypsin treatment. SFs in cell suspensions were cultured in DMEM with 10% FCS.
|
|
|
|
| mSIPCs: mouse Spleen derived Interferon Producing Cells
|
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|
|
| mSDCs are sorted for: Cd11c-pos Ly6c-pos Cd11b-neg
|
|
|
|
| mSkMFibros: mouse Skeletal Muscle Fibroblasts
|
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| from 4-week-old mice muscle samples 18697935(M)
|
|
|
|
| mSkMs: mouse Skeletal Muscle cells
|
|
|
|
| 4-week-old mice were killed by cervical dislocation, and lower limb muscles were carefully dissected away from the bone. Muscle samples were minced with scissors and digested with c ollagenase-Dispase solution (500 mg Collagenase B [Roche]; 50 ml Dispase II (Roche); 250 ul 0.5 M CaCl2). Myoblasts were filtered through a 0.22-uM membrane, spun at 300 X g for 5 min, resuspended in Hams Complete Media (HCM; Hams F-10 Media supplemented with 20% FCS, 2.5 ng/ml bovine FGF, penicillin, and streptomycin) and enriched by "preplating" onto a standard 100-mm culture plate for 1 h. Nonadherent myoblasts were transferred to a 60-mm collagen-coated plate in HCM. After approximately 48 h in culture, the myoblasts were preplated again for 20 min. It took approximately 10-14 days to generate enough myoblasts for experimentation. 18697935(M)
|
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|
|
| mSMacs: mouse Spleen derived Macrophages
|
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|
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| mSCs are positively selected for Cd11b 15665823(M)
|
|
|
|
| express[RT-PCR]: TLR3 TLR4 TLR5 TLR7 TLR9 15665823-Fig-S4a
|
|
|
|
| mSCs are cultured for 6 days in Csf1, adherent cells are used 15322147(M)
|
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|
|
| mSNKCs: mouse Spleen derived NK cells
|
|
|
|
| purified from spleens by positive selection with anti-Cd49b Ab 15345224(M)
|
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|
| slightly pos for TLR9-mRNA [RT-PCR]
|
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|
|
| mSpDCs: mouse Spleen derived Plasmacytoid Dendritic Cells
|
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|
|
| mSCs sorted for: Cd11c-pos B220-pos
|
|
|
|
| used in 12672047(M) 14976261(M)
|
|
|
|
| pos for TLRs 1,2,4,5,6,7,8,9 mRNA [RT-PCR] 12672047(D)
|
|
|
|
| neg for TLR3 mRNA [RT-PCR] 12672047(D) 15711573(D)
|
|
|
|
| pos for TLR7 mRNA[RT-PCR] 15711573(D)
|
|
|
|
| mSCs sorted for: Cd11c-low Ly6c-high B220-pos
|
|
|
|
| 15800576(M) cited by 15492225(M)
|
|
|
|
| mSCs negatively selected for: Cd5 and Cd19 (removes T and B cells)
|
|
|
|
| sorted to be: Cd11c-med B220-pos 15800576(M)
|
|
|
|
| mSCs enriched for Cd11c-pos cells
|
|
|
|
| sorted for: CD11c-pos B220-pos CD3e-neg CD19-neg NK1.1-neg
|
|
|
|
| mSTCs: mouse Spleen derived T-Cells
|
|
|
|
| mSCs sorted for: Cd3-pos NK1.1-neg 15345224(M)
|
|
|
|
| negative for TLR9-mRNA [RT-PCR]
|
|
|
|
| markers: Cd8-pos TCR-Vb5-pos
|
|
|
|
| cells are collected from spleens and/or lymph nodes
|
|
|
|
| negative selection for: CD19, B220, Mac-1, Gr-1, DX5 and CD4
|
|
|
|
| mThyCs: from mouse Thymus
|
|
|
|
| Cd4-pos Cd8-neg 10.1% 15665823-Fig-S1c
|
|
|
|
| Cd4-neg Cd8-pos 3.8% 15665823-Fig-S1c
|
|
|
|
| Cd4-pos Cd8-pos 83.1% 15665823-Fig-S1c
|
|
|
|
| rCMCs: rat Cardiac Myocytes
|
|
|
|
| rEFs: rat Embryo Fibroblasts
|
|
|
|
| rEHNs: rat Embryonic Hippocampal Neurons
|
|
|
|
| used in 12194869(M) refers to
|
|
|
|
| Rameau, G.A., et al. (2000). Role of NMDA receptor functional domains in excitatory cell death. Neuropharmacology 39, 2255–2266
|
|
|
|
| rECNs: rat Embryonic Cortical Neurons
|
|
|
|
| used in 12194869(M) refers to
|
|
|
|
| Rameau, G.A., et al. (2000). Role of NMDA receptor functional domains in excitatory cell death. Neuropharmacology 39, 2255–2266
|
|
|
|
| rFPECs: rat Fat Pad Endothelial Cells
|
|
|
|
| used in 11029289(M) without explanation
|
|
|
|
| rHepatocytes: rat Hepatocytes
|
|
|
|
| Rat livers were digested with collagenase and seeded on type I collagen coated dishes. Hepatocytes were culutred overnight in hapatocyte basal medium supplemented with growth factors.
|
|
|
|
| rNCMs: rat Neonatal Cardiac Myocytes
|
|
|
|
| rSCGNs: rat Superior Cervical Ganglion Neurons
|
|
|
|
| Primary cultures of rat sympathetic neurons were generated from dissociated SCG of postnatal-day-1 Sprague-Dawley rats as described previously (39). The cells were plated onto collagen-coated 24-well dishes at a density of around one ganglion per well and maintained in RPMI 1640 medium supplemented with 10% heat-inactivated donor serum and 60 ng of mouse NGF per ml. A mixture of uridine and 5-fluorodeoxyuridine (10 M each) was added on the following day to eliminate nonneuronal cells.
|
|
|
|
| 39. Lee, V. M., M. L. Shelanski, and L. A. Greene. 1980. Characterization of antisera raised against cultured rat sympathetic neurons. Neuroscience 5:2239–2245.
|
|
|
|
| express mRNA for Mlk1 Mlk2 Mlk3 Dlk Lzk GAPDH 11494869(D)
|
|
|
|
| do not express mRNA for cyclophilin 11494869(D)
|
|
|
|
| rThyrocytes: rat thyroid cells
|
|
|
|
| used in 9038168 with ref to
|
|
|
|
| 27. Meinkoth, J. L., Goldsmith, P. K., Spiegel, A. M., Feramisco, J. R., and Burrow,
G. N. (1992) J. Biol. Chem. 267, 13239 –13245
|
|
|
|
| rVSMCs: rat Vascular Smooth Muscle Cells
|
|
|
|
| from rat aorta 14523024(C)
|
|
|
|
| 36. Melaragno, M. G., Wuthrich, D. A., Poppa, V., Gill, D., Lindner, V., Berk, B. C.,
and Corson, M. A. (1998) Circ. Res. 83, 697–704
|
|
|
|
| As described (3), adipocytes were prepared by collagenase digestion of rat epididymal fat
pads, suspended in glucose-free Krebs-Ringer phosphate (KRP) buffer. 10464253(M)
|
|
|
|
| 3. Standaert, M. L., Galloway, L., Karnam, P., Bandyopadhyay, G., Moscat, J.,
and Farese, R. V. (1997) J. Biol. Chem. 272, 30075–30082
|
|
|
|
| bAECs: bovine aortic endothelial cells
|
|
|
|
| are these primaries or a cell line???
|
|
|
|
| "from donor veal calf descending aortae"
|
|
|
|
| bLUCs: bovine luteal cells
|
|
|
|
| cEFs: chicken embryo cells
|
|
|
|
| 36. Reynolds, A. B., Roesel, D. J., Kanner, S. B., and Parsons, J. T. (1989) Mol.
Cell. Biol. 9, 629 – 638 PMID:2469003
|
|
|
|
| bPAECs: calf (bovine) Pulmonary Artery Endothelial cells
|
|
|
|
| used in 11029289(M) without explanation
|
|
|
|
| sPAECs: sheep Pulmonary Artery Endothelial Cells
|
|
|
|
| SPAEC were cultured from sheep pulmonary arteries obtained from a nearby slaughterhouse as previously described (Hoyt et al., 1995). The SPAECs were grown in OptiMEM (GIBCO) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 Ag/mL streptomycin at 37 -C in an atmosphere with 5% CO2. 16423564(M)
|
|
|
|
| Hoyt, D.G., Mannix, R.J., Rusnak, J.M., Pitt, B.R., Lazo, J.S., 1995. Collagen is a survival factor against LPS-induced apoptosis in cultured sheep pulmonary artery endothelial cells. Am. J. Physiol. 13, L171 – L177.
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|
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