Using KAcAb:

		Using KAcAb-ppt:

		Using 3H-acetate:

		Using 3H-acetate/CHX:

		Using 14C-acetyl-CoA:

		Using AcAb:

		Using WB:

		Using ELISA:

		Using FarWestern:

		Using MamTwoHybrid:

		Using SPR:

		Using Xlink:

		Cells are treated with formaldehyde to cross-link proteins to gene-promoters.

		Cells are lysed and chromatin is sheared by sonication or enzymic digestion.

		Subject is immunoprecipitated from sheared lysates.

		Immunoprecipitates are heated to reverse cross-links.

		Protein is removed from samples with Proteinase-K.

		Amount of Gene in the sample is determined by PCR using primers to Gene-promoter.

		A western blot of a cell lysate, immunoprecipitate, or recombinant protein is stained with an antibody that recognizes the cleavage product of subject.

		A western blot of a cell lysate, immunoprecipitate, or recombinant protein is stained with an antibody that recognizes the cleavage product of subject cleaved at a specific site.

		Using IHC:

		Using FRET:

		Using EM: 

		Using WB:

		Using Xlink:

		Lysates or immunoprecipitates are run on non-denaturing PAGE gels, blotted, and stained for Subject.

		Note: If the dectected Protein complex is approximately twice the molecular weight of the uncomplexed Protein, then the assay is called "dimerization".  Otherwise, "oligomerization" is used.

		Limitations: Does not distinquish between a complexes of Subject:Subject and Subject:OtherProtein.

		Cells are transfected with a plasmid expressing the Subject fused to the DNA binding domain of Gal4 and another plasmid expressing Luc or CAT driven by a promoter containing a Gal4 response element.

		The amount of Luc or CAT expressed is measured by activity assays.

		Subject is preloaded with labeled GDP.

		Subject is incubated with a putative guanine nucleotide exchange factor.

		The amount of GDP released is detected by:

		Subject is incubated with labeled GTP and a putative guanine nucleotide exchange factor.

		Subject is bound to nitrocellulose and washed to removed unbound GTP

		The amount of GTP bound is detected by:

		Using WB:

		Using FRET:

		Using 32P-release:

		Using Pi-release:

		Using TLC:

		Cells are preincubated with radioactive inorganic phosphate (32Pi).

		Subject is immunoprecipitated from cell lysates.

		Nucleotides are eluted from the immunoprecipitates and separated by TLC.

		The amount of radioactivity in the GTP vs GDP spots is calculated.

		Cells are lysed and fractionated (see Fractionation section for methods and putative locations)

		The amount of Subject in each fraction is determined by Western blot.

		Using IHC:

		Using Surface-strip:

		An immunoprecipitate is incubated with a recombinant GTP-binding Protein preloaded with labeled GDP

		The amount of GDP released is detected by:

		More than one Protein (separated by commas) can be used as substrate to represent coupled reactions.

		Using 32P-ATP:

		Using phosAb:

		Using pYAb:

		Using InGelKinase:

		More than one lipid (separated by slashes) can be used as substrates

		An immunoprecipitate is incubated with lipid-substrate in a kinase buffer containing required co-factors and [32P]-ATP.

		Reaction components are separated by Thin Layer Chromatography (TLC).

		Production of phosphorylated lipid substrate is measured by autoradiography.

		Cells are transfected with a plasmid expressing the Subject fused to the DNA binding domain of LexA and another plasmid expressing Luc or CAT driven by a promoter containing a LexA response element.

		The amount of Luc or CAT expressed is measured by activity assays.

		Cells are stained for Subject.

		Position of Subject in respect to cell is determine by microscopy

		Total RNA is prepared from the cells.

		Relative amounts of mRNA are detected using one of the possible DetectionMethods.

		Using NEreporter:

		Using HeterokaryonAssay:

		Using IVnucImport:

		the name and sequence of the oligo are supplied in the oligo field if available

		Using EMSA:

		Using ELISA:

		Using 2TagPpt:

		Using IHC:

		Using density-gradient:

		Using 32P-ATP:

		Using 32Pi:

		Using WBMS:

		Using WBMS/PPase:

		Using phosAb:

		Using phosAb-ppt:

		Using 2D-PPMap:

		Using HPLC-PAA-ED:

		Subject is detected by Western Blot.

		A long smear rather than a single band is indicative of polymerization

		Cells are transfected with a plasmid expressing Luc or CAT driven by the promoter of the Subject.

		The amount of Luc or CAT expressed is measured by activity assays.

		Using WB:

		Using FACS:

		Using 35S:

		Using ELISA:

		using 35S-pulse-chase:

		using CHX-WB:

		note: the subject of a reporter assay of this type is inferred from the sequence of the reporter plasmid itself

		future work will include hunting down the actual sequence of the reporters used and finding evidence that the reporters report what the authors say they do.

		Cells are transfected with a plasmid expressing Luc or CAT driven by the promoter.

		The amount of Luc or CAT expressed is measured by activity assays.

		The concentration of Subject in the supernatant is analyzed by one of the possible DetectionMethods.

		Using WB: